WORLD AQUACULTURE 2015 Aquaculture For Healthy People, Planet and Profit
May 26-30, 2015 Jeju Exhibition & Convention Center Jeju, Korea
THE ANNUAL INTERNATIONAL CONFERENCE & EXPOSITION OF WORLD AQUACULTURE SOCIETY ASIAN-PACIFIC CHAPTER, WAS KOREAN CHAPTER, WAS HOSTED BY Korean Society of Fisheries and Aquatic Science Jeju Special Self-governing Province Jeju Special Self-governing Provincial Council Jeju Fish-Culture Fisheries Cooperatives
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ASSOCIATE SPONSORS Aquaculture Engineering Society International Association of Aquaculture Economics & Management
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Next generation sequencing uncovers unexpected pathogens associated with early mortality syndrome (EMS) in shrimp Kallaya Sritunyalucksana1, Anuphap Prachumwat1, Sissades Tongsima2, Siripong Thitamadee3, Timothy W. Flegel4
Shrimp-virus Interaction Laboratory (ASVI), National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Bangkok 10400, Thailand 2 Biostatistics and Informatics Laboratory, Genome Institute National Center for GeneticEngineering and Biotechnology, Pathum Thani 12120, Thailand 3 Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand 4 National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand E-mail: kallaya@biotec.or.th 1
Presented in "Next Generation Sequencing for Genetic and Genomic Studies (NGS2014)" organized by Mahidol University, 29-30 July, 2014 Winser Hotel, Bangkok, Thailand
pathogens, we also found the concurrent problems in shrimp associated with EMS including the occurrence of aggregated transformed microvilli (ATM). Preliminary study using the next generation sequencing and bioinformatic analysis suggested that there might be the pathogen involved. Further investigations on these pathogens are urgently required in order to design the appropriate strategy to control EMS in shrimp industry.
ACKNOWLEDGEMENTS:
We thank National Research Council of Thailand (NRCT), National Center for Genetic Engineering and Biotechnology (BIOTEC) and Agricultural Research Development Agency (ARDA) for grant support for the study on shrimp EMS.
NEXT GENERATION GENOMIC STUDIES
The most threatening new problem in Asian shrimp aquaculture since 2009 has been serious mortality within approximately the first 35 days after stocking shrimp ponds with post larvae. The problem is commonly called early mortality syndrome (EMS) and started at the early of 2012 in Thailand and has caused Thai shrimp production to reduce 54% in 2013. The cause of EMS was originally unknown. We carried out a metagenomic analysis to determine whether any unique bacteria (culturable or unculturable) might be present in EMS ponds when compared to normal ponds. DNA was extracted from pooled HP tissue samples from normal and EMS shrimp ponds and subjected to PCR amplification of small subunit ribosomal RNA (ssu rRNA) gene fragments using universal bacterial primers. After primer and multiplex adapter sequence trimming, the remaining 59.7 million bases from 163,102 reads with lengths of at least 100 bp were used to construct unique sequences (operational taxonomic units, OTUs). Since no OTU were found to be exclusive to EMS ponds, we compared the proportion of reads for OTU between EMS ponds and normal ponds. This comparison revealed 32 OTUs of six genera from four algorithms that had a significantly higher proportion of reads in EMS ponds than in normal ponds. This comparison revealed OTU with high identity to expected Vibrio species (e.g., V. parahaemolyticus, V. harveyi and V. campbellii) and with high identity to other bacteria known from aquatic environments, but never previously reported as shrimp pathogens. The latter included the genera Delftia and Ralstonia from the order Berkholderiales and Leifsonia and Rhodococcus from the Order Actinomycetales. Preliminary studies with bioassay confirmed that these bacteria could be direct shrimp pathogens. Besides the bacterial
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Case 11-041. Normal hepatopancreas (HP)
Uneaten Feed Bacteria No2 Nitrite
Mineralization
Bacteria
Algae bloom
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5th - 7th NOVEMBER
PRELIMINARY PROGRAM
FIACUI-LACQUA 2014
Hotel Presidente Intercontinental Guadalajara. Guadalajara, Jalisco, Mexico.
Schedule subject to change without notice
LYNX HALL
AQUARIUM HALL
ANTARES HALL
PERSEUS HALL
SCORPIO HALL
ARIES HALL
Wednesday, November 5th 07:00 - 09:00 08:00 - 16:00 09:00 - 09:40 09:40 - 10:20 10:20 - 13:00
13:00 - 15:00 15:00 - 17:00
17:00 - 17:20 17:20 - 19:00
19:30 - 22:00 17:00 - 20:00
Registration & Inscriptions + Posters Assembly. Tradeshow Assembly. MASTER CONFERENCE: "Effects of Climate Change in Aquaculture", Adalberto Val - Director of the National Institute for Amazonian Research, INPA, Brazil. Recess & Coffee break, Lobby. Aquaculture of Indigenous Sustainable Aquaculture. Crustaceans Aquaculture. Mollusks Aquaculture. General Contributions to Ecology & Limnology. - Macrobrachium and other Species. - For a changing World. -Reproduction. Aquaculture. crustaceans. - Financial Integration of - Genetics. - Moderator: Patricia Valenti, sustainable models. CAUNESP, Brazil; Eduardo Ballester, UFRG, Brazil; Fernando Villasante, - Moderator: Sergio Guevara, - Moderator: Carlos Martinez, - Moderator: Adalberto Val, - Moderator: Helcio Marques, CESAIBC, Mexico. UdeG, Mexico. INPA, Brazil. UMSNH, Mexico. APTA, Brazil. Lunch. Aquaculture Economics. Education, Training & Technology Mollusks Aquaculture. Micro-Algae. Aquaculture of Indigenous Sustainable Aquaculture. Transfer. - Nutrition. -Multi-trophic integrated Species. - Moderator: Newton Rodrigues, - Moderator: Pilar Sanchez, - Moderator: Fco. Javier - Grow-out. aquaculture. Martinez Cordero, CIAD, Mexico. Brazil. CICESE, Mexico. - Moderator: Luiz Poersch, Brazil. Recess & Coffee break, Lobby. Sustainable Aquaculture. Mollusks Aquaculture. Fish Larviculture. Aquaculture Economics. Aquaculture in LA&C. Macro-Algae. - Aquaponics. - Environmental & Health - Moderator: Wilfrido Contreras, - Organic Aquaculture. Managment. UJAT, Mexico; Antonio Garza, - Moderator: Ma. Celia Portella, - Moderator: Carlos Leon, BoFish, Mexico; LACC-WAS. Mexico; Felipe - Moderator: Pilar Sanchez, Manuel Segovia, CICESE, Mexico. CAUNESP, Brazil. Matias, FAO, Brazil. CICESE, Mexico. Welcome Cocktail. TRADESHOW SCHEDULE.
Thursday, November 6th 08:00 - 10:00 10:00 - 11:00 11:00 - 11:40 11:40 - 12:00 12:00 - 13:00
Registration & Inscriptions + Posters Assembly. OFFICIAL INAUGURATION BY FEDERAL AND LOCAL AUTHORITIES. MASTER CONFERENCE: "Strategies for the Development of Aquaculture in Mexico", TBA. Recess & Coffee break, Tradeshow. Tilapia Aquaculture. Crustaceans Aquaculture. Feeds & Additives. Marine Finfish Aquaculture. - Reproduction & Genetics. - Nutrition. - Reproduction & Genetics. - Moderator: Lorenzo Juarez, - Moderator: Wilfrido Contreras, ANCPN, Mexico; Luis Andre UJAT, Mexico; Kevin Fitzsimmons, - Moderator: Teresa Viana, Sampaio, UFRC, Brazil; Juan U. of Arizona, USA. UABC, Mexico. Pablo Lazo, CICESE, Mexico. 13:00 - 15:00 Lunch. Feeds & Additives. Marine Finfish Aquaculture. 15:00 - 16:40 Tilapia Aquaculture. Crustaceans Aquaculture. - Larviculture. - Integrated Multi-trophic Aquaculture. 16:40 - 17:00 Recess & Coffee break, Tradeshow. Crustaceans Aquaculture. 17:00 - 18:40 Tilapia Aquaculture. - Health.
Feeds & Additives.
Marine Finfish Aquaculture. - Nutrition.
- Moderator: Scott Horton, Nutrimar, Mexico.
Fish Morphology & Physiology.
Production Systems. - Biofloc.
- Moderator; Wilson Wasielesky, UFRC, Brazil; Sergio Zimmermann, - Moderator: Roberto Mendoza, Zimmermann AquaSolutions, Brazil. UANL, Mexico. Production Systems. - Biofloc.
Fish Morphology & Physiology.
Production Systems. - Recirculation. - Moderator: Marcelo Shei, PREAMAR, Brazil; Michael Schwarz, Virginia Tech, USA.
Gender Inclusion in Aquaculture. - Moderator: Felipe Matias, FAO, Brazil.
10:00 - 20:00 TRADESHOW SCHEDULE.
Friday, November 7th 09:00 - 11:00 Fish Feeding & Nutrition.
Crustaceans Aquaculture. Health.
- Moderator: Juan Pablo Lazo, - Moderator: Scott Horton, CICESE, Mexico. Nutrimar, Mexico. 11:00 - 11:20 Recess & Coffee Break, Tradeshow. Crustaceans Aquaculture. 11:20 - 13:00 Fish Feeding & Nutrition. - Nutrition.
13:00 - 15:00 Lunch. Crustaceans Aquaculture. 15:00 - 17:40 Use of Fish Meal & Fish Oil. - Moderator: Juan Pablo Lazo, - Nutrition. CICESE, Mexico; Albert Tacon, Aquatic Farms, USA. 17:40 - 19:00 CLOSING EVENT, TRADESHOW. 10.00 - 20:00 TRADESHOW SCHEDULE.
@FIACUI
Fish Health.
Production Systems. - Recirculation. - Moderator: Marcelo Shei, - Moderator: Francisco Arregui, PREAMAR, Brazil; Michael S.P.-Bagre, Mexico. Schwarz, Virginia Tech, USA. Catfish, Carp & other Freshwater Species.
Reproduction & Genetics.
Fish Health.
Catfish, Carp & other Freshwater Species.
Ornamental Species. Reproduction & Genetics. - Moderator: Marcelo Shei, PREAMAR, Brazil; Nuno Simões, UNAM, Mexico; Serafin Almenara, AZOO Mexico, Mexico.
Fish Health.
Salmon & Trout Aquaculture.
Ornamental Species.
- Moderator: Camilo Pohlenz, BIOMAR, Costa Rica.
Career Seminary for Students. - Moderator: Juan Pablo Lazo, CICESE, Mexico; Ma. Celia Portella, CAUNESP, Brazil.
- Moderator: Luis Hector Hernandez, UNAM, Mexico.
Panorama Acuicola Magazine Aquaculture Magazine
@PanoramAcuicola @AquacultureMag
- Moderator: Ricardo Ribeiro, UEM, Brazil; Roberto Solis, Acuaplan, Mexico.
Panorama Acuicola Magazine Aquaculture Magazine
BOOTH INFORMATION: Carolina Márquez, servicioaclientes@globaldp.es | International Sales: Steve Reynolds, marketing@dpinternationalinc.com and Mario Stael at mario@marevent.com | General Information: Mario Stael, mario@marevent.com
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BRAZILIAN SHRIMP NEWS
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Reduction in farm density was element in over outbreak in Chile (A. Alvial).
A regional professional network in aquaculture established in 2009, the AAN’s mission is to help aquaculturists and farmers operate a profitable and environmentally sound business, and in doing so, provide the world affordable products at high quality in a sustainable manner.
WORLD AQUACULTURE 2015 WILL FOLLOW THE VERY SUCCESSFUL FIRST KOREAN MEETING - WORLD AQUACULTURE 2008 IN BUSAN World Aquaculture 2015 will be held in Jeju Island – one of the leading flatfish production centers in the world. The rest of the growing aquaculture industry in Korea and the Asian Pacific region will also be represented. A major international trade show at WORLD AQUACULTURE 2015 is the place to learn about the latest in aquaculture, see the newest technology presented by exhibitors from around the world.
Stay in touch with the program developments for WORLD AQUACULTURE 2015 through our Web Page at: www.was.org
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