ageLOC Science in review
18 Total Patents 3 Awards Received 8 Published Scientific Articles
AGELOC SCIENCE IS A GLOBAL PHENOMENON 14 Oral Presentations 26 Scientific Posters
“Nu Skin, with ageLOC science, stands apart from the competition because our approach is based upon 30 years of breakthrough anti-aging research. Our research continues to gain recognition and is validated through presentations at global scientific conferences.”—Joe Chang, Ph.D.
Helsinki, Finland
Corvallis, OR USA
New York, NY USA
Santa Barbara, CA Washington D.C, Phoenix, USA AZ USA Ventura, Atlanta, GA, CA Anaheim, USA CA USA New Orleans, LA USA
Berlin, Brussels, Germany Belgium Paris, Les Diablerets, France Switzerland Barcelona, Spain
Siena, Italy
Sapporo, Tokyo, Japan Hangzhou, Japan China
Wuhan, China Hong Kong, China
Taipei, Taiwan
Bangkok, Thailand Singapore Buenos Aires, Argentina
ageLOC Science 2011 Timeline
Exploratory Study to Assess Association of Skin Aging with Plasma Isoprostane Levels in Healthy Middle-aged Japanese Women Anne Lynn S. Chang, M.D., Tiffany C. Chang1, Bharathi Lingala1, Ph.D., Dale G. Kern, MS2, Steve M. Wood, Ph.D.2, Hidekazu Toyoda, M.S.2, Helen E. Knaggs, Ph.D.2 1Stanford University School of Medicine,Department of Dermatology, 2Nu Skin International
RESULTS
RESULTS
BACKGROUND
RESULTS (continued) Sk in Age vs 8-ISO-PGF2a
aging is hypothesized to be oxidative stress.
To date, quantification of oxidative stress in human organ systems has been difficult.
Characteristic
SkinAge > CA (n=26)
SkinAge <= CA (n=20) p-value
Skin Age
57.5 (5.35)
46.5 (5.66)
<0.001
Chronological Age
51.04 (4.93)
50.9 (4.93)
0.925
100
Plasma 8-Iso-PGF2a levels
One of the central mechanisms of
OR Estimate/ 10 unit change in Isoprostane
80
64.27 (11.46)
47.7 (8.76)
<0.001 36
38
40
42
44
46
48
50
52
54
56
58
60
62
64
66
68
Ski n Age in years
PGF2a
136.18 (64.1)
65.19 (19.28)
<0.001
BMI
20.92 (3.38)
22.81 (2.56)
0.0365
Education Level
2.81 (0.69)
2.95 (0.76)
0.52
UV Exposure
0.38 (0.85)
0.05 (0.22)
0.095
Stress level at work
2.15 (1.95)
2.55 (2.06)
0.51
Coffee intake per day
1.04 (0.66)
1 (0.9)
0.87
Tea intake per day
1.23 (0.76)
1.25 (1.02)
0.94
p
0.001 0.001
8-iso-PGF2a
0.10
(0.01 , 0.75)
0.025
PGF2a
0.39
(0.17 , 0.89)
0.026
8-iso-PGF2a PGF2a
20
OBJECTIVE
95 % CI
(0.09 , 0.52) (0.34, 0.77)
Un-adjusted: 40
Iso_PGF2a
0.22 0.51
60
Adjusted:
Chronological Age vs 8-ISO-PGF2a
Plasma 8-Iso- PG F2a levels
LIMITATIONS This study is limited by sample size.
80
60
40
CONCLUSIONS
20 44
46
48
50
52
54
56
58
60
Plasma isoprostanes merit further study as markers of oxidative stress in the the skin and possible biomarkers of skin aging
Chronological Age in year s
Skin Age vs PGF 2a 40 0
“Yes” to medication usage
35 0
7.70%
Oral supplement use
0.72 ( 1.37)
Fruits/vegetable intake per day
1.69(0.74)
15%
1.10
( 1.55)
0.64
0.39
Plasma PGF2a levels
METHODS IRB approved Inclusion criteria: • women aged 45-60 years old •in general good health as assessed by the investigator Exclusion criteria: •prior use of prescription medications to improve the appearance of photo damaged skin •facial cosmetic procedures •smoking •dermatologic conditions on the face •use of self-tanner two days prior to enrollment
100
30 0 25 0
FUNDING
20 0 15 0 10 0 50
1.80(1.06)
0.69
0 36
38
40
42
44
46
48
50
52
54
56
58
60
62
64
66
68
This study was sponsored by NuSkin International.
Skin Age in year s
“Yes” for meat intake
88.50%
80%
ACKNOWLEDGEMENTS
0.38
Skin aging severity ratings performed by 4 dermatologists using facial photographs and replicated , with Pearson correlation R from ranging 0.82- 0.90
Chronological Age vs PGF2a
We would like to thank Yuko Naritomi for technical assistance and Alan Taylor at Oregon State University for isoprostane analysis.
370 320
Plasm a PG F2a levels
Exploratory study determine if human skin aging severity associates with plasma isoprostane levels, specifically PGF2a, 8-isoPGF2a while controlling for covariates
270 220 170 120 70 20 44
46
48
50
52
54
56
58
60
Chronological Age in years
FEBRUAR Y 2011 Published Article in Cosmetics and Toiletries Presented at American Academy of Dermatology’s 69th Annual Meeting
2 Presented at Fifth International Symposium on Health and the Environment
“This research identifying groups of genes and genetic pathways that change in expression as we age is essential to building our proprietary ageLOC science that targets the sources of aging.”— Joseph Chang, Ph.D., Nu Skin Chief Scientific Officer and Executive VP of Product Development
MARCH Presented at Gordon Research Conference
Presented at Society for Investigative Dermatology Awarded Utah Innovation Award for ageLOC Vitality: Natural Products Category
APRIL
MAY
JUNE
2011 4 Presented at Federation of American Societies of Experimental Biology
Released The Aging Myth, a book by Joseph Chang, Ph.D. Presented Gene Expression Research in Paris, France
Presented at Tenth Symposium of Mycology Societies Published Article in Central China Agriculture University Journal
JULY
Presented at Linus Pauling Institute Diet and Optimum Health Conference 2 Presented at European Society for Dermatological Research Filed Patent on R2
SEPTEMBER
OCTOBER
2011 The Aging Myth Became a New York Times Best Seller
Presented R2 Research at Second International Conference on Environmental Stressors in Biology and Medicine Launched Nutritional Product ageLOC R2
Presented at Fourth International Conference and Exhibition for Nutraceuticals and Functional Foods Presented at 2011 International Conference on Food Factors
NOVEMBER
Figu re 6. Avera ge o f err or in age assessmen t [app aren t age estim atio n â&#x20AC;&#x201C; r eal age] in years + stan dard de viations vs PGF2 Îą concen tration , pg/ml.
Nu Skin Acquired LifeGen Technologies
DECEMBER
2011 Presented at New TCM Product Development and Industrial Development Strategy Summit Published Article in Zhejiang University Journal
Presented at Society of Cosmetic Chemists Annual Scientific Seminar
Published Online article in Dermatological Surgery Presented at International Federation of Societies of Cosmetic Chemists
ageLOC Science Publications and Presentations
FULL LIST Patents
Awards
Multiple Patents in the following categories:
Angela Mastaloudis, Senior Scientist, Received the DSM Nutraceutical Research Award
Licensed Patents-Pending on Formulation or Ingredients Nu Skin-Developed Patents-Pending on Formulation or Ingredients Licensed Patent on Method Nu Skin-Developed Patent-Pending on Method
Remona Gopaul, Associate Senior Scientist, Named Young Scientist of the Year by the Society of Cosmetic Chemists Nu Skin Awarded Utah Innovation Award for ageLOC Vitality: Natural Products category, 2011
FULL LIST 2011 Published Articles February 2011 Gopaul, R., Knaggs, H., Wickett, R. “A Review of Genomic Techniques in Cosmetic Testing.” Cosmetics & Toiletries. February 2011, pp. 112-117. July 2011 Tan, N., Barger, J., Zhang, Y., Prolla, T., Weindruch, R., Zhao, C., Bartlett, M., Zhu, J. “CordyMax Cs-4 restores aging-associated changes in gene expression and extends lifespan in normal aged mice.” Proceeding of the 10th Symposium of Mycology Societies Across Taiwan Strait, Central China Agriculture University Publishing. 2011, pp 255-256.
November 2011 Li, C., Tan, N., Barger, J., Zhang, Y., Ferguson, S., Prolla, T., Zhu, J. “Combined use of ageLOC whole-gene expression profiling technology and mouse lifespan test in anti-aging herbal product study.” Proceeding of New TCM Products Innovation and Industrial Development Summit, Zhejiang University Publishing, Hangzhou. 27 Nov. 2011. (in press) December 2011 Chang, A., Lingala, B., Chang, T., Kern, D., Wood, S., Toyoda, H., Knaggs, H. “An Exploratory Study to Determine the Association Between Assessed Facial Skin Aging and Plasma Isoprostane Levels in Middle-Aged Japanese Women.” Article first published online: 5 Dec. 2011 Dermatological Surgery. http://onlinelibrary.wiley.com/doi/10.1111/j.1524-4725.2011.02235.x/abstract
FULL LIST 2011 Oral Presentations June 2011 “Transcriptional biomarkers of calorie restriction and their modulation by dietary interventions.” Invited Speaker: Free Radicals, Nutrition and Aging: From Fundamental Aspects to Clinical Applications. Paris, France, June 15-17, 2011. Barger, J. July 2011 “CordyMax Cs-4 restores aging-associated changes in gene expression and extends lifespan in normal aged mice.” The 10th Symposium of Mycology Societies Across Taiwan Strait, Wuhan, China, July 17, 2011. Tan, N., Barger, J., Ferguson, S., Zhang, Y., Prolla, T., Zhu, J. September 2011 “Transcriptional biomarkers of calorie restriction in adipose tissue and modulation by dietary interventions.” Invited Speaker: Linus Pauling Institute Diet and Optimum Health Conference. Corvallis, OR, September 13-16, 2011. Barger, J.
October 2011 “A unique nutritional formulation to promote cellular protection and purification via induction of the Nrf2-mediated ARE/Phase II detoxification pathways.” Invited Speaker: Second International Conference on Environmental Stressors in Biology and Medicine. Centro Didattico, Policlinico Le Scotte Siena, Italy. October 5-7, 2011. Mastaloudis, A. November 2011 “Restructuring of the ECM in Human Skin by Equol: A Plant and Soy-Derived Isoflavonoid.” Gene Expression and Protein Evidence presented at the 4th International Conference and Exhibition for Nutraceuticals and Functional Foods, Sapporo, Japan, November 2011. Gopaul, R., Knaggs, H., Lephart, E. “Whole-gene transcriptional profiling to detect aging-associated mitochondria dysfunction and to develop an anti-aging product.” Invited Speaker: The 2011 International Conference on Food Factors, Taiwan, November 20-23, 2011. Zhu, J., Dong, Y., Barger, J., Lu, J., Tan, N., Prolla, T., Weindruch, R., Bartlett, M. “Combination of ageLOC biochip whole-gene expression profiling and mouse lifespan test in CM anti-aging study.” Invited Speaker: Summit
“Combination of ageLOC biochip whole-gene expression profiling and mouse lifespan test in CM antiaging study.” Invited Speaker: Summit of New TCM product Development and Industrial Development Strategy, Hangzhou, China, November 25-27, 2011. Li, C., Tan, N., Barger, J., Ferguson, S., Zhang, Y., Prolla, T., Zhu, J.
FULL LIST Posters 2011 February 2011 “Exploratory Study to Assess Association of Skin Aging with Plasma Isoprostane Levels in Healthy Middle-Aged Japanese Women.” The American Academy of Dermatology’s 69th Annual Meeting, February 2011, New Orleans, LA. Chang, A., Chang, T., Lingala, B., Kern, D., Wood, S., Toyoda, H., Knaggs, H. March 2011 “A PCR-based screen to identify natural compounds with the ability to influence Nrf2-mediated transcriptional induction of detoxification/antioxidant genes.” Gordon Research Conference: Oxidative Stress & Disease. Emerging Research Areas in the Study of Oxidative Stress and Disease. March 13-18, 2011, Ventura, CA. Mastaloudis, A., Barger, J., Prolla, T., Weindruch, R., Ferguson, S., Wood, S. “Cordyceps sinensis Cs-4 restores aging-associated changes in gene expression and extends lifespan in normal aged mice.” Progress in Nutrition 2011. The 5th International Symposium, “Promoting Good Health and Developing a Sustainable Environment,” Hong Kong, March 10-11, 2011. Zhu, J., Tan, N., Barger, J., Zhang, Y., Prolla, T., Weindruch, R., Zhao, C., Bartlett, M. “ageLOC Vitality improves exercise endurance, glucose metabolism and antioxidant capacity in an aging model.” Progress in Nutrition 2011. The 5th International Symposium, “Promoting Good Health and Developing a Sustainable Environment,” Hong Kong, March 10-11, 2011. Zhu, J., Yang, J., Dong, Y., Zhao, C., Wu, Z., Zhang, Y., Tan, N., Lu, J., Bartlett, M.
April 2011 “The lifespan-prolonging effect of Cordyceps sinensis Cs4 in normal mice and its molecular mechanisms.” FASEB J 2011 25(1): 599.1. FASEB EB2011, Washington, DC, Apr. 10, 2011. Tan, N., Barger, J., Zhang, Y., Prolla, T., Weindruch, R., Zhao, C., Bartlett, M., Zhu, J. “ageLOC Vitality improves endurance exercise, exercise glucose metabolism and antioxidant capacity in an aging model.” FASEB J 2011 25(1): 1049.2. FASEB EB2011, Washington, DC, Apr. 12, 2011. Yang, J., Dong, Y., Zhao, C., Wu, Z., Zhang, Y., Tan, N., Lu, J., Bartlett, M., Zhu, J. “Cordyceps sinensis Cs-4 restores aging-associated changes in gene expression and extends lifespan in normal aged mice.” FASEB J 2011 25(1): 1090.13. FASEB EB2011, Washington, DC, Apr. 13, 2011. Tan, N., Barger, J., Zhang, Y., Prolla, T., Weindruch, R., Zhao, C., Bartlett, M., Zhu, J. “An ageLOC Vitality formula improves endurance exercise in aged mice and energy metabolism in muscle mitochondria.” FASEB J 2011 25(1): 1114.9. FASEB EB2011, Washington, DC, Apr. 13, 2011. Dong, Y., Lu, J., Yang, J., Zhao, C., Tan, N., Wu, Z., Zhang, Y., Gao, L., Bartlett, M., Zhu, J. May 2011 “Association of skin aging severity with blood isoprostane levels in healthy middle-aged Japanese women.” Society for Investigative Dermatology (SID) May 2011, J. Invest Dermatol 131. S. 85 (2011). Chang, T., Lingala, B., Kern, D., Wood, S., Toyoda, H., Knaggs, H., Chang, A.
July 2011 “CordyMax Cs-4 restores aging-associated changes in gene expression and extends lifespan in normal aged mice.” Proceeding of the 10th Symposium of Mycology Societies cross Taiwan Strait, Central China Agriculture University. 2011, pp 255-256. Oral presentation at: The 10th Symposium of Mycology Societies Cross Taiwan Strait, Wuhan, China, July 17, 2011. Tan, N., Barger, J., Ferguson, S., Zhang, Y., Prolla, T., Zhu, J. September 2011 “A Multifactorial Approach to Addressing Body Appearance Aging.” European Society for Dermatological Research (ESDR) September 2011, J. Invest Dermatol 131. S. 54 (2011). Knaggs, H., Kern, D., Gopaul, R., Langerveld, A. “A Multifactorial Gene Expression Approach to Understanding Skin Aging.” J. Invest Dermatol 131. S. 57, September 2011. Knaggs, H., Kern, D., Bartlett, M., Wood, S., Mastaloudis, A., Ferguson, S., Langerveld, A. “A Systematic Approach to Evaluate and Rank Natural Ingredients Based on Their Ability to Oppose Age-Related Changes in Gene Expression.” Linus Pauling Institute Diet and Optimum Health Conference. Corvallis, OR. September 13-16, 2011. Wood, S., Mastaloudis, A., Barger, J., Prolla, T., Weindruch, R.
FULL LIST Posters 2011 October 2011 “A unique blend of natural compounds with the ability to oppose age-related changes in gene expression related to dysregulation of removal of cellular debris, cellular detoxification, antioxidant protection and inflammatory balance.” Second International Conference on Environmental Stressors in Biology and Medicine. October 5-7, 2011, Centro Didattico, Policlinico Le Scotte Siena, Italy. Mastaloudis, A., Barger, J., Prolla, T., Weindruch, R., Bartlett, M., Wood, S. November 2011 “Combination of ageLOC biochip whole-gene expression profiling and mouse lifespan test in CM anti-aging study.” Proceeding of Summit of New TCM product Development and Industrial Development Strategy, China J Chinese Materia Medica Publishing. 2011. Li, C.L., Tan, N., Barger, J., Ferguson, S., Zhang, Y., Prolla, T., Zhu, J. Invited Speaker at: Summit of New TCM product Development and Industrial Development Strategy, Hangzhou, China, November 25-27, 2011. “Whole-gene transcriptional profiling to detect agingassociated mitochondria dysfunction and to develop an anti-aging product.” Proceeding of 2011 International Conference on Food Factors. Zhu, J.S., Dong, Y.Z., Barger, J.L., Lu, J.H., Tan, N.Z., Prolla, T.A., Weindruch, R., Bartlett, M. Invited Speaker at: The 2011 International Conference on Food Factors, Taiwan, November 20-23, 2011.
December 2011 “Influencing the Expression of Genes Associated with Skin Inflammation by Combining Narcissus Tazetta Bulb Extract and Schizandra Chinensis Fruit Extract—an in vitro Analysis.” Society of Cosmetic Chemists Annual Scientific Seminar, New York, NY, December 2011. Gopaul, R., Knaggs, H., Kern, D. “So Why Don’t I Look Younger?” International Federation of Societies of Cosmetic Chemists, Bangkok, Thailand, December 14-15, 2011. Kern, D., Chang, A., Wood, S., Gopaul, R., Lephart, J., Knaggs, H.
summary We believe Nu Skin’s ongoing research, combined with the acquisition of LifeGen Technologies, will deepen the company’s understanding of gene expression and its impact on reversing the effects of aging even later in life. With our exclusive focus on identifying, targeting, and resetting Youth Gene Clusters, we believe ageLOC science is the answer to anti-aging. It’s a scientific breakthrough that goes beyond the signs to target the ultimate sources of aging. For more information about ageLOC science, please visit ageloc.com.