GLOBAL LIFE SCIENCE SUPPLY
Short Form Catalogue and Price List 2010
CONTENTS Page No
1
Electrophoresis
5
Electrophoresis Agaroses
6
Histology
7
Scintillation Counting
10
Electro-Optical Cleaning Agents
11
Microbiology Media
18
Peptide/Organic Synthesis Reagents
19
Transfection
20
Plasmid Purification
22
Dithiothreitol
24
Terms & Conditions
Contact Us: AGTC Bioproducts Ltd t/a National Diagnostics Tel: 01482 646020 Fax: 01482 646013 Email: office@agtcbioproducts.com www.agtcbioproducts.com Unit 4 Fleet Business Park, Itlings Lane, Hessle, Hull, HU13 9LX
NATIONAL DIAGNOSTICS
Electrophoresis
1
Featured Products ProtoGel ® Quick-Cast 12%
· · ·
Cast a Gel in One Fourth the Time Ideal for Western Blotting Applications Precast Convenience without the Precast Price
With the introduction of ProtoGel Quick-Cast, the time required for casting gels for protein separation has been reduced from 2 hours to under 25 minutes, and the number of steps involved has been cut from 10 to only 3. With ProtoGel Quick-Cast, simply measure out the amount needed, add initiators and cast the gel. No mixing, no multiple measurements, no need for a second casting to produce a stacking gel. Just initiate, cast, and be ready to run in 20 minutes. With the reduced casting time, western blots can be finished within a regular working day.(NDH) Product Name
Description
Code
Pack Size
Diogenes
Enhances superoxide detection.
CL-202
1 Kit
£178.20
Cl-204
1 Kit
£86.77
CL-205
1 Kit 240 ml
£428.27
CL-205
1 Kit 900 ml
£140.90
CL-252
1 system
£30.89
EC-201
100 grams
£21.88
EC-201
500 grams
£70.76
EC-201
1 kilogram
£124.17
Hydrogen Peroxide Assay Kit HRPL Kit
For enhanced non-isotopic detection of proteins and nucleic acids in Western and Southern blot techniques.
ProtoBlock System
To enhance signal to noise ratio during chemiluminescent membrane bound visualization of Proteins and DNA.
Acrylamide
National Diagnostics' Acrylamide has been specially purified to produce consistent gel matrices for the most demanding requirements.
£
Bis (Methylene BisAcrylamide)
BIS is a crosslinking agent for general acrylamide electrophoretic use. Specially purified.
EC-301
25 grams
£13.79
DATD
DATD is an allyl type crosslinker containing a 1,2-diol structure which may be oxidized by periodic acid.
EC-303
25 grams
£31.55
Glycine
Purified by novel chelation chromatography to remove all reactive metals.
EC-405
250 grams
£9.65
1 kilogram
£25.73
Tris
Base component for PAGE buffer formulation. Purified to remove ammonia and amine contaminants. Results in greater resolution during isoelectric focusing.
EC-406
250 grams
£12.99
1 kilogram
£34.74
Tricine
Ultra pure molecular biology grade reagent used as a base component for protein PAGE buffer formulation.
EC-407
100 grams
£34.74
Ion Exchange Resin
A mixture of anionic and cationic resins specially prepared for the deionization of electrophoretic gel monomer solutions prior to initiation.
EC-408
100 grams
£19.31
Riboflavin
For the photo-polymerization of polayacrylamide electrophoresis gels. Single crystalline species ensures greater reproducibility.
EC-501
25 grams
£19.31
TEMED
Fractionally distilled to remove all trace metals and amine impurities, which inhibit gel formation.
EC-503
25 ml
£12.99
Ammonium Persulfate
Exceeds ACS Standards. Low absorbed water results in consistent initiating capabilities.
EC-504
25 grams
£19.31
100 grams
£19.31
1 gram
£11.57
5 grams
£71.85
25 grams
£85.50
50 grams
£101.18
100grams
£118.97
50 ml
£21.88
100 grams
£19.31
1 kilogram
£85.57
250 grams
£14.80
1 kilogram
£31.54
Dithiothreitol (DTT)
Cleland's Reagent, a reducing agent used in protein chemistry. Specially purified to remove trace metals and other impurities. Low odour formulation. Kilo price on request.
EC-601
2-Mercaptoethanol
Triple distilled to remove impurities and stored under nitrogen to prevent their reformation.
EC-603
SDS
Purified to remove colored contaminants that interfere with spectrophotometric analysis.. Zero free sulfates eliminate non-specific binding.
EC-604
Urea
Recrystallized to remove ammonia. Exclusive purification process has been shown to inhibit ammonia regeneration.
www.agtcbioproducts.com
EC-605
NATIONAL DIAGNOSTICS
2
Electrophoresis ND Protein Precipitation Kit NEW
Recovery of All Proteins from Complex Mixtures Precipitates as Little as 100ng of BSA at 0.25µg/ml Milder than TCA More Reliable than Acetonitrile or Ammonium Sulfate The ND Protein Precipitation Kit is mild on samples and easy to use. It casts the finest net of any procedure, allowing the high yield collection of all proteins in solution, precipitating even the most dilute proteins. The ND Protein Precipitation Kit allows collection of proteins that would be missed by other methods .Ideal for both routine and difficult work, the ND Protein Precipitation Kit stands alone among all methods. It offers the best combination of mildness, simplicity and effectiveness in protein precipitation. Product Name
Description
Code
Pack Size
Glycerol
Electrophoresis grade, for use in loading buffers and to alter the mechanical properties of gels.
EC-606
450 ml
£28.59
Specially purified to remove epoxidized components. Nonionic surfactant used in Western blotting and other protein studies.
1 ltr
£37.95
Tween 20
EC-607 200ml
£25.99
Formamide
Redistilled for absolute purity. DNA denaturing agent.
EC-608
200 ml
£33.45
EC-615-25ML
Formamide ULTRA CE, Specialy purified for caplillary electrophoresis
EC-615
25 ml
18.73
EDTA
Component of electrophoresis buffers. Ultra pure, molecular biology grade reagent.
EC-610
100 grams
£50.18
Glass Bond
National Diagnostics' Glass Bond allows polyacrylamide gels to be temporarily affixed to one of the glass plates used for casting electrophoresis gels.
EC-620
25 ml
£71.29
Glass Free
Coats glass plates to prevent binding of polyacrylamide gels, allowing Convenient disassembly of the cassette after electrophoresis.
EC-621
450 ml
£35.64
GelDry Film 11 X 12 cm
GelDry Film is a specially designed drying film to be used in air-drying procedures for polyacrylamide gels.
EC-612
50 sheets
£23.79
Sterling Rapid Silver Stain
The fastest and most sensitive silver stain to date, the Sterling Rapid Silver Stain Kit produces superb results in one hour.
EC-720
1 kit
£99.72
ProtoBlue Safe
The Best Performing Colloidal Stain - Detects as little as 5ng protein
£
1 liter
£59.35
4 liter
£193.66 £26.17
EC-722
Nuclistain
Nuclistain visualizes in the visible spectrum and is less toxic than ethidium bromide. Detects as little as 50ng of DNA.
EC-730
25 ml
Accugel PAGE
DNA Extraction Kit (50 PREPS)
EC-745
KIT
£60.12
Protprep KIT II
Meltable acylamide matrix
EC-755
KIT
£102.94
Insite System
In-gel fluorescent stain for proteins. The fastest high sensitivity detection of proteins following SDS-PAGE available.
EC-759
1 Kit
£115.81
450 ml
£23.16
AcrylaGel
30% ultra-pure stock solution of acrylamide.
EC-810 1 liter
£30.24
450 ml
£17.38
Bis-AcrylaGel
2% ultra-pure stock solution of bisacrylamide.
EC-820 1 liter
23.16
EC-830
450 ml
£23.80
SequaGel Concentrate
Monomer concentrate component of the SequaGel Sequencing System. EC-830
1 liter
£45.03
SequaGel Sequencing System
System to conveniently formulate 19:1 acrylamide/bisacrylamide sequencing gels up to 20%.
EC-833
1 liter kit
£74.62
equaGel Buffer
Buffer component of the SequaGel Sequencing System.
EC-835
100 ml
£12.87
450 ml
£22.53
SequaGel-6
Convenient systems with 19:1 monomer, urea, TBE, and TEMED for casting 6 percent denaturing gels.
1 liter
£34.74
EC-836
Convenient systems with 19:1 monomer, urea, TBE, and TEMED for casting 8 percent denaturing gels.
EC-838
450 ml
£21.23
EC-838
1 liter
£45.03
SequaGel TTE Complete Buffer
Order separately to run glycerol tolerant gels with SequaGel 6 or 8.
EC-839
200 ml
£23.14
450 ml
£11.57
SequaGel Diluent
Diluent component of the SequaGel Sequencing System.
EC-840 1 liter
£23.16
SequaGel-8
Phone: 01482 646020
Fax: 01482 646013
Email: office@agtcbioproducts.com
NATIONAL DIAGNOSTICS
Electrophoresis
3
ProtoBlue Safe Ÿ
Ÿ
The Best Performing Colloidal Stain - Detects as little as 5ng protein ProtoBlue Safe improves both the sensitivity and the universality of staining. ProtoBlue Safe is less prone to high background caused by trace residual SDS in the gel. Costs Less than Regular Coomassie Laboratories typically spend twice as much per gel in the methanol and acetic acid for staining and destaining than for ProtoBlue Safe (including ethanol cost). In addition, ProtoBlue Safe is much faster, more sensitive, and you can pour the used stain solution down the drain. Protein visualization is faster, safer, more sensitive and less-expensive with ProtoBlue Safe. Non hazardous Disposal.
Product Name
Description
Code
Pack Size
SequaGel Complete Buffer
Component of SequaGel 4, 4.25, 4.75, 6 or 8. No need to order separately.
EC-841
200 ml
£11.57
450 ml
£25.73
1 liter
£45.03
SequaGel XR
Proprietary formulation for extended reads in both manual sequence gels and automated sequencers.
SequaGel XR Concentrate
Proprietary formulation for extended reads in both manual sequence gels and automated sequencers.
EC-843
SequaGel MD Monomer Solution
Proprietary solution for the resolution of minor mutational differences in conformational analysis.
SequaGel MD SSCP Kit
Proprietary solution for the resolution of minor mutational differences in conformational analysis. Contains matrix concentrate and SSCP Stop Solution.
SequaGel MD Heteroduplex Kit SequaGel MD SSCP Stop Solution AccuGel 19:1 (40%)
AccuGel 29:1 (30%)
AccuGel 29:1 (40%)
EC-842
£
100 ml
£41.81
450 ml
£115.80
EC-845
200 ml
£86.20
EC-846
1 kit
£89.41
Proprietary solution for the resolution of minor mutational differences in conformational analysis. Contains matrix concentrate and Triple Dye Loading Buffer.
EC-847
1 kit
£89.41
Formamide loading buffer.
EC-848
1.2 ml
£10.30
450 ml
£30.24
1 1iter
£46.32
450 ml
£30.24
1 1iter
£46.32
450 ml
£30.24
EC-852
1 liter
£46.32
1.2 ml
£16.09
1 liter
£21.23
4 liters
£47.61
40% stock molecular biology grade solution of acrylamide/ bisacrylamide, 19:1 ratio. 30% stock molecular biology grade solution of acrylamide/ bisacrylamide, 29:1 ratio. 40% stock molecular biology grade solution of acrylamide/ bisacrylamide, 29:1 ratio.
EC-850
EC-851
Triple Dye Loading Buffer (6X)
Three dyes (Xylene Cyanol, Bromophenol Blue, and Orange G) allow continuous monitoring of electrophoretic runs.
EC-855
TBE 10X
Commonly used gel and tank buffer for DNA electrophoresis.
EC-860
TBE 5X
Commonly used gel and tank buffer for DNA electrophoresis.
EC-861
4 liter
£25.73
TE BUFFER 100X
Concentrated solution of 1 M Tris-HCl, pH 8, with 100 mM EDTA, the standard for DNA and RNA purification and storage.
EC-862
25 ml
£33.45
Tris-Tricine-SDS PAGE Buffer (10X)
SDS-PAGE tank buffer specifically designed to improve the resolution of small proteins (5 - 20 kD)
EC-869
1 liter
£82.36
Tris-Glycine-SDS PAGE Buffer (10X)
Ultra-pure, standardized Laemmli running buffer for SDS PAGE of proteins.
EC-870
1 liter
£20.58
4 liter
£38.60
TTE Glycerol Tolerant Buffer 20X
For making glycerol tolerant PAGE gels, this buffer must be used with ProtoPrep for DNA electrophoresis.
EC-871
1 liter
£34.74
TAE Buffer 50X
Ultra-pure preformulated buffer for electrophoresis when sample recovery is important.
EC-872
1 liter
£36.03
SSC Buffer 20X
National Diagnostics SSC Buffer (20X) is optimized for Northern or Southern Capillary Blotting.
EC-873
1 liter
£19.40
4 liters
£48.26
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NATIONAL DIAGNOSTICS
4
Electrophoresis
Buffer Solutions In the gel and in the tank, the purity and strength of buffers determine not only the electrical parameters of electrophoresis but also the structure and solubility of the bio-molecules under study. Buffer quality is as important for electrophoresis results as the gel matrix itself. Produced to the most exacting standards in the industry, National Diagnostics’ buffers are stable and pure. They eliminate weighing and mixing, save time, and help ensure reproducible results. Stringent Quality Control - 18 Mega ohm Water - 0.2 Micron Filtration - Save Money - Save Time - Improve Results
Ÿ Ÿ Ÿ Ÿ Ÿ Ÿ
Ÿ
Tris-Glycine Electroblotting Buffer (10X) TBE Buffer (10X or 5X) Triple Dye Loading Buffer (6X) Capillary Electrophoresis Buffer (10X) Tris-Glycine-SDS PAGE Buffer (10X) Tris-Trycine-SDS PAGE Buffer (10X) ProtoGel Buffers
Ÿ Ÿ Ÿ Ÿ Ÿ Ÿ
Ÿ
TAE Buffer (50X) TTE Glycerol Tolerant Buffer (20X) Protein Loading Buffer Blue (2X) TE Buffer (100X) Neutralization Solution Denaturation Solution SSC Buffer (20X)
Product Name
Description
SDS Solution 20%
National Diagnostics SDS Solution 20% saves time, is more stable and provides more reproducibility than making up this solution in the lab.
Code
Pack Size
£
100 ml
£16.09
EC-874
Denaturation Solution
Ready-to-use, ultra-pure solution of NaCl and NaOH for Southern and Northern Blotting, and in situ hybridization procedures.
EC-875
450 ml
£33.45
1 liter
Neutralization Solution
Returns gels to pH 7 after denaturation, prior to Southern capillary blotting.
£15.44
EC-876
1 liter
Dextran Sulfate
Aids in formation of networks (high localized concentrations of probes) during hybridization, thus expediting the annealing process.
£15.44
Tris-Glycine Electroblotting Buffer 10X
Optimized for electrophoretic transfer of proteins to PVDF, Nitrocellulose or Nylon membranes in Western Blotting.
EC-877
50 grams
£75.27
1 liter
EC-880
£16.72
4 liter
Protein Loading Buffer Blue 2X
Ready to use buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE.
£51.47
EC-886
10x1 ml
£18.66
EC-888
1 kit (50 ml)
£73.63
ND Protein Precipitation Kit
Protein Precipitation Kit
ProtoGel
· · · ·
Recovery of All Proteins from Complex Mixtures Precipitates as Little as 100ng of BSA at 0.25µg/ml Milder than TCA More Reliable than Acetonitrile or Ammonium Sulfate
Stabilized, 30% acrylamide/bis solution (37.5:1 ratio) optimized for SDSPAGE of proteins.
EC-890
450 ml
£36.66
1 liter
£61.12
450 ml
£46.40
1 liter
£77.33
450 ml
£19.31
ProtoGel 40%
Stabilized, 40% acrylamide/bis solution (37.5:1 ratio) optimized for SDSPAGE of proteins.
EC-891
ProtoGel Resolving Buffer
Ready-to-use buffer to formulate standard Laemmli resolving gels of .375 M Tris-HCl and 0.1% SDS, pH 8.8.
EC-892
ProtoGel Stacking Buffer
Ready-to-use buffer to formulate standard Laemmli stacking gels of .125 M Tris-HCl and 0.1% SDS, pH 6.8.
EC-893
ProtoGel QC 12%
Ready-to-use gel solution for SDS-PAGE that combines the convenience of pre-cast gels with the economy of casting your own.
EC-895
Insite Markers
Prestained markers combined with unstained, engineered standards. Ideal markers for fluorescent detection methods.
EC-897
0.5 ml
ProtoMarkers
Prestained protein markers for Western Blot and SDS-PAGE combining highcontrast visualization and easy orientation.
EC-898
0.5 ml
£94.56
ProtoMetrics
Non-stained, engineered protein markers
EC-899
1 Tube
£115.80
Phone: 01482 646020
Fax: 01482 646013
1 liter
28.94
200 ml
£15.44
100 ml 450 ml
£16.53 £57.49 £77.20
Email: office@agtcbioproducts.com
5
AGTC Bioproducts STANDARD (ROUTINE) AGAROSE NEW AND IMPROVED Hi-Res Standard (EEO 0.13%)-D1
Our new production method has produced a superb medium for separating nucleic acid fragments < 23 Kb. This agarose is also suitable for Blotting Assays, Presence of DNAses and RNAses not detected. There is no better value agarose than this. Hi-Res Standard GQT (Genetic Quality Tested)-D1-GQT Highly purified agarose, with low levels of EEO, GQT (Genetic Quality Tested). Recommended for DNA preparatory gels. Presence of DNAses and RNAses not detected.
Electrophoresis Agarose Product Code
Size
AG-D1-25
25 gm
£17.63
Price
AG-D1-100
100 gm
£60.31
AG-D1-500
500gm
£261.72
AG-D1-1k
2 x 500gm
£380.69
AG-D1-25-GQT
25 gm
£18.50
AG-D1-100-GQT
100 gm
£63.00
AG-D1-500-GQT
500gm
£270.00
AG-D1-1K-GQT
2 x 500gm
£410.00
Hi-Gel Standard (D2)
Mega-Base Rapid (High Strength Gel)-(D5)
Recommended agarose for Agarose Beads preparation, it can also be used for protein electrophoresis and counter immunoelectrophoresis. Presence of DNAses and RNAses not detected.
Specific agarose for pulsed fields electrophoresis, recommended for DNA > 1000 bp separation, by conventional electrophoresis and for DNA with a size of several megabases by ”Pulse Field Gel Electrophoresis”. Presence of DNAses and RNAses not detected.
Product Code
Size
Price
AG-D2-25
25 gm
£17.63
Product Code
Size
AG-D2-100
100 gm
£60.31
AG-D5-25
25 gm
£52.29
AG-D2-500
500 gm
£261.72
AG-D5-100
100 gm
179.51
AG-D2-1k
2 x 500 gm
£395.00
Price
LOW MELTING AND GELLING POINTS
MS (MOLECULAR SCREEN) HIGH RESOLUTION
Hi-RES Low Melt-LM2
Hi-Res Super + -MS4 Recommended for analytical separation of DNA <500 bp. Presence of DNAses or RNAses not detected, no DNA binding detected.
Recommended agarose for <1000 bp fragment separation in analytical and preparatory electrophoresis. The low melting point of this agarose (65oC) allows the gel to melt without damaging the DNA. Presence of DNAses and RNAses not detected.
Low Melt Mega Clone-LMGQT GQT (Genetic Quality Tested) Especially recommended for preparatory electrophoresis of nucleic acids >1.000 bp and In-Gel applications.
Low Melt Clone-LMSVE Very low melting point agarose has a higher resolution capability for fragments <1.000 bp, especially for PCR products, between 200-800 bp.
Hi-Res Super-MS8 Recommended for analytical separation of DNA < 1000 bp particularly useful for PCR products. Presence of DNAses or RNAses not detected, no DNA binding detected.
Hi-Strength Analytical - MS12 Recommended for analytical separation of DNA. At 2% concentration it can separate fragments of 100-1500 bp. Presence of DNAses or RNAses, no DNA binding detected. Product Code Size Price AG-MS4-50
50gm
£90.00
AG-MS4-100
100 gm
£174.88
Product Code
Size
Price
AG-MS4-250
250 gm
£451.00
AG-LM2-25
25 gm
£76.56
AG-MS8-25
25 gm
£51.41
AG-LM2-100
100 gm
£242.19
AG-MS8-100
100 gm
£174.88 £639.56
AG-LM2-25-GQT
25 gm
£86.85
AG-MS8-500
500gm
AG-LM2-100-GQT
100 gm
£346.19
AG-MS12-25
25 gm
£90.00
AG-LMSVE-25
25 gm
£59.49
AG-MS12-100
100 gm
£239.83
AG-LMSVE-100
100 gm
£196.30
AG-MS12-500
500gm
£639.56
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6
Histology
Histo-Clear®
NATIONAL DIAGNOSTICS
Ÿ Rapid Clearing without the Toxic Hazards of Xylene Ÿ Biodegradable Ÿ Greatly Reduces Disposal Costs Ÿ Superior Results with Lower Citrus Odour Ÿ Natural Product with Food Grade Rating World-Wide
The use of Histo-Clear in the laboratory means no longer having to breathe xylene when preparing histological sections. Distilled from oranges, carefully purified, and stabilized, Histo-Clear is one of the safest clearing agents available. Histo-Clear not only improves safety but also results. Histo-Clear leaves tissue less hard and brittle than xylene, facilitating the cutting of thin sections and pro‐ longing microtome blade life. Nuclear morphology is rendered in fine detail. Histo‐Clear enhances the clarity and vibrancy of acidophilic stains and improves staining of Harris’ Hematoxylin with a brighter Eosin background. Histo-Clear can directly substitute for xylene and yields excellent results in automated tissue processing. Product Name
Description
Code
Histosol
High Flash point solvent replacement for xylene as a histological clearing agent. Completely miscible with all commonly used histology reagents including Ethanol, Isopropanol, t-butanol and all embedding and mounting media.
HS-100
Mirsky's Fixative
Mirsky's fixative enhances tissue penetration for Immunohistochemistry and Immunocytology. Produces high definition rendering of tissue using microwave fixation.
Histomount
Permanent UV stabilized mounting medium for preparation of permanent mount museum quality slides. Neutral pH. Dispensing device included.
Calci-Clear
For high quality bone and tissue decalcification, utilizing chelating and sequestering agents to efficiently remove solubilized calcium. Ideal where maintenance of structural integrity is paramount.
HS-102
HS-103
HS-104
Pack Size 1 gallon
£ £14.28
5 gallon
£73.76
200 ml system
£12.87
2 liter system
£32.16
100 ml
£9.78
450 ml
£21.88
1 quart
£11.19
1 gallon
£34.36
1 quart
£9.78
1 gallon
£34.36
HS-106
100 ml
£24.71
HS-108
5 gallon pail
£24.96
Calci-Clear Rapid
For very rapid, high quality bone and tissue decalcification. Tissue distortion and structure loss is minimized without loss of speed.
HS-105
Hydromount
High quality water based mounting medium ideal for temporary mounts, immunofluorescence, staining procedures and frozen sections.
Neutralin
Neutralin converts hazardous aldehyde waste into a nonhazardous polymer and water.
Histo-Clear
A nontoxic (food grade), low flammability, biodegradable solvent, Histo-Clear substitutes for xylene as a histological clearing agent. Histo-Clear improves safety and results.
HS-200
Histo-Clear II
With reduced citrus odor compared to citrus based clearing agents, HistoClear II is a low toxicity, reduced flammability, biodegradable solvent, substituting for xylene as a histological clearing agent.
HS-202
Alcian Blue
Histological stain used to differentiate acid and neutral mucopolysaccharides and as a differential stain for amyloid tissue.
Biebrich Scarlet
Biebrich Scarlet can be used as a plasma stain instead of Acid Fuchsin in Masson s trichrome.
Fast Green FCF
Recommended as a substitute for Light Green SF yellowish in Masson s trichrome because it is less likely to fade. It may be substituted for Light Green in many other procedures as well.
Basic Fuchsin
Basic Fuchsin is the main ingredient of Schiff s reagent and can be used as stain for glycoproteins and mucopolysaccharide proteins in acidic pH systems.
HS-518
25 grams
£16.72
Methylene Blue
Sensitive stain specific for DNA and RNA.
HS-525
25 grams
£23.16
1 gallon
£32.12
5 gallon
£153.46
1 gallon
£21.42
5 gallon
£96.36
HS-504
25 grams
£17.38
HS-506
25 grams
£11.33
HS-516
25 grams
£19.31
Amido Black 10B
Stain for proteins in gels and on Western Blots.
HS-601
25 grams
£21.23
Bromocresol Green
Tracking dye for DNA electrophoresis in agarose.
HS-602
5 grams
£22.25
Bromophenol Blue
Tracking dye for DNA or protein electrophoresis.
HS-603
10 grams
£17.24
Coomassie Blue R-250
Sensitive stain for proteins on polyacrylamide gels.
HS-604
10 grams
£11.57
Coomassie Blue G-250
Stain for proteins on polyacrylamide gels. Assay for proteins in solution.
HS-605
10 grams
£11.57
Methyl Green
Methyl Green in conjunction with Pyronin will differentiate RNA & DNA.
HS-606
10 grams
£21.23
Pyronin Y
Pyronin in conjunction with Methyl Green will differentiate RNA & DNA.
HS-607
5 grams
£39.50
Xylene Cyanol FF
Tracking dye for DNA electrophoresis.
HS-608
25 grams
£35.64
Phone: 01482 646020
Fax: 01482 646013
Email: office@agtcbioproducts.com
Scintillation Counting
7
NATIONAL DIAGNOSTICS
For nearly 40 years National Diagnostics liquid scintillation cocktails have provided a stable basis for all your counting needs. Our high quality modern formulated cocktails and secondary reagents such as tissue solubilizers and specialty chemicals meet the very highest standards of performance; enhancing user safety and convenience. In addition our unique and detailed manual of techniques can help you with even the most challenging liquid scintillation counting requirements.
Choosing a Scintillation Fluid for Your Sample or Application Sa m pl e s a n d Applications
b e s t www.agtcbioproducts.com
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Scintillation Counting Ecoscint Ultra Ÿ Ÿ Ÿ Ÿ Ÿ
8
NATIONAL DIAGNOSTICS
TM
When National Diagnostics laboratories set out to
The Best Performing Cocktail on the Market Biodegradable Ultra-High Sample Holding (1 to 1.2 Cocktail to Sample) Ultra-Low Background Unsurpassed Counting Efficiency
create Ecoscint Ultra, our aim was to create the best cocktail
available
environmental counting
and
for
sample urine
bioassay. We are proud to announce that not only have we succeeded in this goal, we have surpassed it, creating the best all-around performing cocktail on the market for both large and small aqueous samples. Ecoscint Ultra enables ultra-low level counts to be discriminated from background not only for large but also for small samples. With large samples, Ecoscint Ultra delivers up to 30% 3H counting efficiency at maximum sample hold with very low background levels. With small samples, Ecoscint Ultra yields 3H efficiency greater than 60%.10ml Ecoscint Ultra holds 12ml H2O in a clear emulsion at 18C o. 10ml Ecoscint Ultra holds 10ml 0.5M NaCl at that temperature. Product Name
Description
Code
Pack Size
Hydrofluor
Hydrofluor is a high efficiency, nonflammable liquid scintillation solution designed to count large volumes of aqueous samples. Hydrofluor offers a gel phase option for counting particulate samples.
LS-111
4 Gal
Liquiscint
Liquiscint is an economical, ready-to-use liquid scintillation solution designed for aqueous and organic samples, a good all around performer.
LS-121
Betafluor
Betafluor provides high efficiency counting of non-aqueous samples.
LS-151
Monofluor
Monofluor provides extremely high sample holding capacity, accomplishing a clear continuous liquid phase up to 50% sample. Monofluor utilizes a nonflammable solvent base.
Filtron-X
£ £39.12
1 Gal
£31.00
5 Gal
£149.14
1 Gal
£23.68
5 Gal
£88.14
LS-191
4 liter
£57.64
A scintillation fluid which solubilizes cellulose acetate, cellulose nitrate and mixed ester filter disks assuring homogeneous counting.
LS-201
4 liter
£54.18
Oxosol C14 Oxidizer
Oxosol C14 is a complete scintillator designed to absorb and count 14CO2 produced by sample combustion.
LS-211
1 gallon
£88.13
Oxosol 306
Oxosol 306 is a complete scintillation solution specifically formulated to count 14CO2 samples trapped in Carbamate.
LS-231
1 liter
£88.14
Carbamate-1 CO2 Absorber
Carbamate-1 is a high capacity 14CO2 absorber intended to be used in conjunction with Oxosol 306.
LS-241
450 ml
£200.00
LS-270
4 liter
£68.87
Ecoscint Ultra An excellent all around performer, Ecoscint is biodegradable and nonflammable, with comparable efficiency and greater sample holding capacity than conventional scintillators.
LS-271
Ecoscint XR
Ecoscint XR is the best performing ultra-high sample hold fluid available. Ideal for environmental testing or any application requiring very high sample volume.
LS-272
Ecoscint A
Readily biodegradable with high flash point, low odor and low toxicity, Ecoscint A combines high sample hold with high efficiency.
LS-273
Ecoscint O
For nonaqueous samples, Ecoscint O exhibits lower backgrounds and improved quench resistance. Ecoscint O is readily biodegradable with high flash point, low odor and low toxicity.
LS-274
Ecoscint H
Ecoscint H provides the maximum counting efficiency for aqueous sample volumes under 10%. Ecoscint H is readily biodegradable with high flash point, low odor and low toxicity.
LS-275
Uniscint BD
Uniscint BD is a biodegradable scintillation solution especially formulated to accommodate high salt and buffer samples.
LS-276
Ecoscint
Phone: 01482 646020
Fax: 01482 646013
4 liter bottle
£64.34
20 liter drum
£194.17
4 liter bottle
£82.45
4x4 liter case
£320.00
4 liter bottle
£55.97
20 liter drum
£264.13
4 liter bottle
£34.22
20 liter drum
£170.89
4 liter bottle 20 liter drum 4 liter bottle
£57.51 £271.87 £65.22
Email: office@agtcbioproducts.com
NATIONAL DIAGNOSTICS TM
Ecoscint Flow
Scintillation Counting
9
Ÿ Non hazardous and Biodegradable
Formulated
Ÿ Low Viscosity
applications, Ecoscint Flow represents the
Ÿ Non-Gelling
utilization of National Diagnostics’ new
Ÿ Ultra-High Sample Hold (1:1
advances in scintillation fluid chemistry to
Cocktail to Sample) Low Toxicity Ÿ High Counting Efficiency
for
multipurpose
flow
create a low viscosity cocktail. Ecoscint Flow accepts a wide range of HPLC gradients at a 1:1 ratio, providing high counting efficiency (25% H3 efficiency at
1:1 cocktail/sample). Even difficult samples such as 0.1N NaOH mix rapidly to yield a clear, non-viscous emulsion.
Product Name
Description
Code
Monoflow 2
Flow scintillation cocktail for routine, low-salt, aqueous effluents.
LS-282
Monoflow 3
Monoflow 4
Ecoscint Flow
Bioscint
Flow scintillation cocktail providing high sample holding capacity for routine, low salt effluents. Flow scintillation cocktail for high salt aqueous amples. Can accomodate 2M salt gradients. Ecoscint Flow is a low viscosity, ultra-high sample hold, biodegradable scintillation cocktail ideal for HPLC flow counting.
Biodegradable Tissue Solubilizer/ Scintillator Combination Non hazardous Waste Clear Emulsions for Excellent Counting
Biosol
Pack Size
£
4 liter bottle
£57.64
20 liter drum
£269.16
4 liter bottle
£57.64
20 liter drum
£261.05
4 liter bottle
£64.34
20 liter drum
£287.95
1x4 liter bottle
£82.45
20 liter drum
£194.87
LS-309
1x4 liter bottle
£93.28
LS-310
400 ml
£64.34
LS-283
LS-284
LS-288
Solusol
A superior solubilizing agent which may be used with a wide variety of samples.
LS-311
450 ml
£94.83
Soluscint XR
Companion Cocktail for Solusol Ideal for High Salt and Alkaline Samples
LS-314
4 liter
£57.64
Autofluor
Water soluble scintillation phosphor for autoradiographic image intensification. Safer and more effective than PPO-DMSO.
LS-315
1 liter
£92.64
Bottle-top Dispenser
LS-900
1 Dispenser
P.O.A.
Extendable Delivery Jet
LS-904
1 Jet
P.O.A.
1 quart
Nuclean
Concentrated, effective, economical solution for removing radioactivity from laboratory glassware, equipment, and surfaces.
NC-200
Nuc-Wipes
Unique pads for environmental wipe tests which dissolve in scintillation fluid, ensuring accurate, reproducible results.
Scintillation Vials 6 mL Scintillation Vials 8 ml
(HDPE) scintillation vials manufactured as a one-piece moulding with no seams, proving excellent UV light transmission for high counting efficiency.
Scintillation Vials 20 mL
www.agtcbioproducts.com
£8.10
1 gallon
£29.21
NW-300
1 box (100/box)
£13.52
SVC-06
1 case (2500)
SVC-08
1 case (2000)
SVC-20
1 case (1000)
£81.58
£62.15
Electro-Optical Cleaning Agents
10
NATIONAL DIAGNOSTICS
To reduce the environmental impact and occupational health and safety hazards associated with toxic organic clearants in the laboratory and in industry, National Diagnostics developed its original, award-winning OptiClearTM cleaning solvent. As a result of ever growing demand for safe products for cleaning optical and electronic components, National Diagnostics expanded the OptiClear product line to include an array of lower hazard solvents.
Product Name
Description
Code
16 oz. spray bottle
Award winning, nontoxic, nonflammable, biodegradable solvent, intended for the removal of wax, pitch, flux, grease, and resin from optical and electronic components of glass, ceramic, or metal.
OE-101
OptiClear R
Water based solvent intended as an acetone replacement for cleaning optical and electronic components.
OE-102
OptiClear E
Our fastest evaporating OptiClear formulation, for the removal of wax and pitch.
OptiClear
OptiClear E OptiClear S
OptiClear W Opticlear S2
OptiClear S has a higher flash point and evaporates more slowly than OptiClear, for the removal of wax and pitch.
Pack Size
£ £24.96
1 gallon
£40.52
5 gallon drum
£195.94
2 oz. squeeze bottle
£8.49
16 oz. spray bottle
£21.23
1 gallon
£38.20
OE-104
16 oz. spray bottle
£25.47
OE-104
1 gallon
£42.46
16 oz. spray bottle
£25.47
OE-105
OptiClear W is a nontoxic, nonflammable, biodegradable solvent intended to remove grease and oil from optical and electronic components. OptiClear W is miscible in all proportions in water.
OE-106
Our highest flash point solvent, for the removal of wax and pitch.
OE-107
1 gallon
£42.46
5 gallon drum
£172.92
16 oz. spray bottle
£29.72
1 gallon
£53.02
5 gallon drum
£193.03
16 oz. spray bottle
£29.72
1 gallon
£54.30
Opticlear Sample Kit I
1 liter each of OptiClear, OptiClear R, OptiClear S, OptiClear W.
OE-108
1 liter each
£22.91
Opticlear Sample Kit II
1 liter each of OptiClear, OptiClear E, OptiClear R, OptiClear S, OptiClear W.
OE-109
1 liter each
£31.70
Phone: 01482 646020
Fax: 01482 646013
Email: office@agtcbioproducts.com
11
AGTC Bioproducts
Microbiology Media
Ÿ C o m p r e h e n s i v e M i c r o b i o l o g y Protocol C a t a l o g u e Ÿ Large selection of Pre-Formulated Growth Media Ÿ Extensive range of Peptones and Supplements AGTC Bioproducts are pleased to be able to bring you some of the best quality products for bacterial growth, identification and manipulation We are one of the world leaders in the distribution of high quality culture media, we are able currently, to offer more than 400 different products, among which you will find chromogenic media, ISO-formulated media and custom-made media for many different industrial applications. From hygiene control, through food and beverage comtamination prevention, to microbiologial examination of cosmetic and pharmaceutical products, We supply a wide variety of different media for each field so that customer are able to find the most suitable product for their needs. We comply with ISO 9001 , and our products comply with various international standards such as ISO, the European Pharmacopoeia, FDA, APHA, USP and AOAC standards. Strict quality control procedures are adopted prior to, during and after
the manufacturing process to ensure quality products and batch-to-batch consistency. We also exert tight control over selection and treatment of all raw materials and components (peptones, carbohydrates, minerals, chemicals, agar and other additives) used in the manufacturing process. Physical-chemical characteristics are tested, and media also undergo additional microbiological tests that guarantee growth, differentiation, biochemical performance, recovery of small binocular, selectivity, etc. A quality control certificate is supplied with each product. Our reputation and success throughout the world are the result of a combination of uncompromising quality systems, technical expertise and innovative thinking.
CLINICAL AND INDUSTRIAL MICROBIOLOGY Code
Product
AG-1100
BAIRD PARKER AG-AR BASE
AG-1108
BLOOD AG-AR BASE
AG-1048
Price (500 g)
Code
Product
£65.66
AG-1062
MANNITOL SALT AG-AR
£25.09
£43.05
AG-1058
MUELLER HINTON AG-AR
£44.33
BRAIN HEART INFUSION AG-AR (BHI AG-AR)
£69.64
AG-1240
RAPPAPORT SOY BROTH
£39.18
AG-1010
BRILLIANT GREEN AG-AR
£90.14
AG-1081
ROSE BENGAL AG-AR
£44.41
AG-1402
BUFFERED PEPTONE WATER
£31.29
AG-1220
SELENITE CYSTINE BROTH
£50.57
AG-1104
COLUMBIA AG-AR BASE
£36.92
AG-1056
STANDARD METHODS AG-AR (P.C.A)
£37.57
AG-1027
CONFIRMATORY K.A.A. AG-AR
£67.41
AG-1070
STREPTOCOCCUS SELECTIVE AG-AR
£40.76
AG-1028
DNAse TEST AG-AR
£67.08
AG-1068
T.S.A.
£35.19
AG-1508
FLUID THYOGLICOLLATE MEDIUM (FTM)
£36.42
AG-1224
T.S.B.
£29.07
AG-1203
GLUCOSE BROTH
£39.07
AG-1092
V.R.B.G.
£29.07
AG-1052
MAcCONKEY AG-AR
£35.24
AG-1093
V.R.B.L.
£36.87
PEPTONES + AGAR Code
Product
AG-1616
BACTERIOLOGICAL PEPTONE
£38.02
AG-1800
EUROPEAN BACTERIOLOGICAL AGAR
£51.48
AG-1600
MEAT PEPTONE
£46.49
AG-1612
TRYPTONE
£48.76
www.agtcbioproducts.com
Price (500 grams)
Price (500 g)
12
Microbiology Media
AGTC Bioproducts
Most important for this industry is the pathogen Listeria monocytogenes. This gram-positive bacteria has been the cause of numerous outbreaks of foodborne diseases in the last decade. Listeriosis is usually found in pregnant women, their fetuses and in people with immune problems; symptoms are commonly abortion, Meat and fish products are highly perishable. The neonatal death, septicemia and meningitis, with type of microorganisms present is variable and a mortality rate exceeding 20%. very much depends on the product. Although the muscle itself is virtually sterile, meat-based Listeria is usually found in most food, but the processed foods are very susceptible to highest rates are in meat, poultry and seafood. It contamination providing favorable conditions for survives periods of cold storage and it grows at growth of microorganisms that can cause temperatures as low as 0°C. It also survives at high salt concentrations for long periods of time and is poisoning and food-borne illness. relatively resistant to dehydration.
Meat and Fish Industries
The increase in international trade in recent years, has made necessary the use of faster, more efficient and more specific microbiological diagnostic culture media for each individual industry and each pathogen.
We can offer a wide range of specific culture media for the meat and fish industry.
Some specific media for Listeria: Code
Description
Size
Price
AG-1182
LISTERIA FRASER BROTH BASE (ISO 11290-1)
500 grams
£58.15
AG-1133
LISTERIA AGAR BASE OXFORD: Detection .
500 grams
£79.30
AG-1141
LISTERIA AGAR BASE PALCAM: Selective differential medium for detection .
500 grams
£70.48
AG-1345
LISTERIA CHROMOGENIC AGAR BASE : Selective medium for detection and enumeration.
500 grams
£110.13
AG-1120
LISTERIA ENRICHMENT BROTH BASE FRASER (ISO 11290-1): Detection and isolation.
500 grams
£52.86
AG-1398
TRYPTONE SOY YEAST EXTRACT AGAR (TSYEA) (ISO 11290-1): Growth of Listeria spp.
500 grams
£37.22
AG-1339
TRYPTONE SOY YEAST EXTRACT BROTH (TSYEB) (ISO 11290-1): Confirmation of Listeria monocytogenes . 500 grams
£29.85
Some media for the identification of other pathogens important to meat industry safety: Size
Price
AG-1400 BRAIN HEART INFUSION BROTH (B.H.I. BROTH): Confirmation of positive-coagulase Staphylococcus . AG-1219 BRILLIANT GREEN SELENITE BROTH II : Selective enrichment of Salmonella species .
Code
Description
500 grams 500 grams
£47.41 £73.67
AG-1044 LYSINE IRON AGAR : Studies of decarboxylation of lysine for rapid differentiation of Salmonella arizonae.
500 grams
£56.52
AG-1356 PSEUDOMONAS AGAR BASE (ISO 13720) : Isolation, detection and enumeration of Pseudomonas .
500 grams
£44.90
AG-1096 ROGOSA SL AGAR: Selective media for cultivation of Lactobacilli.
500 grams
£50.13
AG-1234 ROGOSA SL BROTHS: Elective media for cultivation of Lactobacilli.
500 grams
£38.66
AG-1064
SALMONELLA SHIGELLA AGAR (S.S.): Selective medium for isolation of Salmonella and Shigella .
500 grams
£47.06
AG-1082
S. P.S AGAR: Isolation of Clostridium perfringens .
500 grams
£61.74
AG-1029
TSC AGAR BASE (ISO 7937): Isolation of Clostridium perfringens .
500 grams
£46.26
AG-1075
TSN AGAR: Isolation of Clostridium perfringens .
500 grams
£51.03
Phone: 01482 646020
Fax: 01482 646013
Email: office@agtcbioproducts.com
AGTC Bioproducts
13
Water & Beverages Natural water supplies such as rivers and lakes and streams contain sufficient nutrients to support growth of various organisms. Microorganisms enter the water supply in many different ways. In congested centers water supplies are polluted by domestic and industrial waste. As a potential carrier of pathogenic microorganisms, water can endanger health and life. Drinking water is obtained from different sources. Various impurities may occur in these waters that have to be removed before the water is supplied to the public. To render the water safe for drinking it has to be treated property. Proper sampling is essential to provide representative aliquots to the laboratory conducting the tests. Microorganisms are living organisms. In addition, when found in water, they are not a perfect solution, but a suspension with a degree of variability that is inherent to it. The presence and level of fecal contamination is an important factor in assessing the quality of water bodies and the risk of infection posed to human health. The analysis of water samples to
Microbiology Media
detect Escherichia coli, which normally inhabits the intestinal tract of humans and other warm-blooded animals, provides an indication of this type of contamination. Other indicators of fecal contamination are Enterococci. Such microorganisms are particularly important when the contamination occurred some time ago and the less resistant coliform bacteria, including Escherichia coli, may have died by the time the analysis is performed. Pseudomonas aeruginosa is an opportunistic pathogen for humans that is capable of growing in water with very low nutrient concentrations. It is desirable that natural mineral waters, spring waters, bottled waters and waters for human consumption are free of Pseudomonas aeruginosa. Sometimes, for reasons of public health the presence of Pseudomonas in other types of water may be investigated, including water in swimming pools. For the identification of all these key pathogens in the analysis of water we suggest the following media: Water in general:
Code
Description
AG-1071
R2A AGAR (Eur. Pharma.) Recommended by the European Pharmacopoeia for total aerobe count in treated water.
Code
Description
AG-1005 BILE ESCULIN AZIDE AGAR (ISO 7899-2): Provides rapid growth and efficient recovery of Enterococci MACCONKEY AGAR WITHOUT CRYSTAL VIOLET: Investigation of enteric microorganisms, especially Enterococci, from AG-1037 water. AG-1109 SLANETZ-BARTLEY MEDIUM (ISO 7899-2): Enumeration of Enterococcus faecalis in water systems.
Size
Price
500 grams
£49.46
Size
Price
500 grams
£91.10
500 grams
£35.19
500 grams
£60.99
AG-1412 M-EI AGAR BASE CHROMOGENIC: detection and enumeration of Enterococci in water membrane-filtration technique . 500 grams
£155.95
Code
Price
Description
Size
AG-1127 FECAL COLIFORMS AGAR BASE (m-FC); Membrane-filter medium for the detection and enumeration of fecal Coliforms . 500 grams £50.77 AG-1357 PEPTONE WATER WITH LACTOSE (ISO 9308-1): confirmation of Coliforms in water . TRYPTONE SOY AGAR (ISO 9308-1): Detection and enumeration of E.coli and other Coliform bacteria by membraneAG-1138 filtration . AGTTC CHAPMAN AGAR (SO 9308-1: Recommended for the enumeration of Coliforms in drinking water by membrane A51076 filtration technique .
Code
Description
KING B MEDIUM (PSEUDOMONAS F AGAR) (USP): Identification and enumeration of Pseudomonas aeruginosa by membrane-filtration . AG-1060 NUTRIENT AGAR: Identification and enumeration of Pseudomonas aeruginosa by membrane-filtration PSEUDOMONAS CN AGAR BASE (UNE-EN 12780): Identification and enumeration of Pseudomonas aeruginosa by AG-1153 membrane-filtration .
AG-1532
Code
Description
500 grams £37.04 500 grams £35.40 500 grams £34.67
Size
Price
500 grams
£47.28
500 grams
£40.70
500 grams
£73.07
Size
Price
CLOSTRIDIUM PERFRINGES AGAR BASE (m-CP) : Used in the two-step membrane-filtration method for detection of 500 grams £71.22 Clostridium perfringens in environmental water and all other types of water . LEGIONELLA CYE AGAR BASE : Optimum for the cultivation of Legionella with shorter incubation periods from AG-1311 500 grams £60.00 environmental and clinical samples. YEAST EXTRACT AGAR (ISO 6222):these are nutrient medium for enumeration of a wide spectrum of bacteria, yeast and AG-1049 500 grams £38.24 molds . AG-1132
www.agtcbioproducts.com
Microbiology Media Dairy products
Chemical analysis carried out on dairy products are of considerable importance in the quality control of finished products, for example to determine the freshness period of a product, or its expiration date. Food safety is essential in milk and dairy products industry. The healthy image that these products have, particularly the perception of their safety and quality, is always under close scrutiny due to outbreaks of human illnesses and product recalls implicating milk, cheese, ice creams, etc.
Code
AGTC Bioproducts
14
Therefore, thorough testing with the correct culture media is essential to ensure safety for consumption. For the identification of these pathogens we have selected several typically specific culture media for this industry:
Description
Size
Price
Isolation and identification of Group D Streptococci. Foods of animal or vegetable origin obtained by fermentation may have a very high content of group D streptococci, which are present as natural flora. These AG-1005 BILE ESCULIN AZIDE AGAR (ISO 7899-2): Streptococci can be used as indicators of highly resistant enteric pathogens. They are also useful in the analysis of thermally processed foods and in determining the effectiveness of disinfection and cleaning systems.
500 grams
£91.10
BRYANT & BURKEY BASE BROTH AG-1247 (modified with resarzurine):
Detection of lactate fermenting clostridial species, in milk and dairy products, particularly Clostridium tyrobutyricum. This bacterium is responsible for the "late spoilage" in certain types of cheese and it gives them an unpleasant taste and odor, which comes to crack and even pop cheese.
500 grams
£58.65
AG-1301 CHLORAMPHENICOL AGAR (ISO 7954):
Selective medium for isolation and enumeration of molds in milk and dairy products. Recommended by ISO.
500 grams
£39.55
AG-1020 DESOXYCHOLATE AGAR :
Isolation and enumeration of Coliforms in dairy products.
500 grams
£38.77
AG-1254 E.S.T.Y. BROTH:
Selective media for cultivation of Streptococcus thermophilus in yogurt. Presence of this microorganism is the main difference between traditional yogurts and the yogurts "pasterized after fermentation" (that don't need cold temperature), which don't contain it.
500 grams
£44.76
500 grams
£48.87
500 grams
£42.48
Selective media for isolation and identification of yeasts and molds in dairy products.
500grams
£26.22
Isolation and confirmation of Lancefield Group D Streptococci in foods. (see Bile Esculin Agar).
500grams
£67.41
500 grams
£87.85
500 grams
£28.86
AG-1555 E.S.T.Y. Medium: s AG-1094 GLUCOSE CHLORAMPHENICOL AGAR AG-1258
GLUCOSE CHLORAMPHENICOL BROTH
AG-1027 K.A.A. CONFIRMATORY AGAR AG-1209 K.A.A. PRESUMPTIVE BROTH AG-1445
LAURYL SULPHATE TRYPTHOSE BROTH MODIFIED (m LST) (ISO 22964)
AG-1318 M 17 AGAR
Selective enrichment medium for Enterobacter sakazakii (see Enterobacter sakazakii Isolation Chromogenic Agar). cultivation and enumeration of lactic Streptococci in milk and dairy products. Recommended for isolation of Streptococcus thermophilus in yogurts. Also for cultivation and maintenance of cheese and yogurt fermentations.
AG-1238
ROTHE BROTH (Glucose Broth with Azide)
For the quantitative determination of fecal Streptococci.
AG-1033
STANDARD METHODS AGAR WITH POWDERED MILK (ISO 4833)
For bacterial plate counts of microorganisms from milk and dairy derivatives (APHA* Formula).
Phone: 01482 646020
Fax: 01482 646013
500 grams
£61.05
500 grams
£39.46
500 grams
£44.71
Email: office@agtcbioproducts.com
AGTC Bioproducts
Microbiology Media
15
contamination is more likely to happen in spring and early summer and in places of high dust or flour concentration. Bread fungi are aerobic and vegetative microorganisms, meaning they need oxygen to reproduce themselves, so it is common for fungi to proliferate first in the crust, which is the area most exposed to air inside the package.
Bakery industry Within the broad spectrum of the food industry, one of the most commonly consumed daily article is bread. Today, both industrial as well as traditional bakeries use additives to control the growth of bacteria and molds in their products, but to meet legal requirements and adhere to hygiene standards during preparation they must also test for microorganisms. The prior control of raw materials and their control during the preparation process is vital to obtaining a final product free of microorganisms.
For the identification of all these pathogens of importance in the safety of the bakery industry, there are several important culture media:
After baking, the environment makes an ideal culture medium for the development airborne spores, so it is vitally important to treat this type of product in its final format. This kind of Code
Description
Size
AG-1022 POTATO DEXTROSE AGAR (Eur. Pharma.)
identification, cultivation and enumeration of yeasts and molds. The content of potato (a source of starch) and the low pH favor the growth 500 grams of those yeasts and molds selective medium for the enumeration of yeasts and molds in food. Bengal rose is a selective agent that inhibits the growth of bacteria and limits the size and height of faster-growing molds, allowing for the 500 grams development of other slower-growing fungi.
AG-1160
ROSE BENGAL AGAR + DICHLORAN + CLORAMPHENICOL (DRBC AGAR)
AG-1527
O.G.A. MEDIUM (Oxytetracycline Glucose Agar)
AG-1124 BACILLUS CEREUS SELECTIVE AGAR BASE
enumeration and selection of yeasts and molds in food samples. enumeration and isolation of Bacillus cereus in food, according to MOSSEL.
Price £34.99
£59.31
500 grams
£29.46
500 grams
£38.02
Why you should use our media for ISO 6579:2002 ISO 6579:2002 specifies a horizontal method for the detection of Salmonella, including Salmonella Typhi and Salmonella Paratyphi. It is applicable to products intended for human consumption and the feeding of animals, and to environmental samples in the area of food production and food handling. Everybody involved in these areas should conform to this standard when testing for microbial contamination. P RE - ENRICHMENT
S ELECTIVE
Buffered peptone water Cat No. 1402 RVS Broth Cat No. 1174
MKTTn Broth Cat No. 1173
ENRICHMENT
PLATE
C ONFIRMATION
XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122 XLD Agar Cat.1274 & Salmonella Chromogenic Agar Cat.1122 Biochemical Tests
Salmonella
Urea Indol TSI Agar Cat 1172. β Galactosidase Lisine Decarboxilase Broth Cat No 1176 Voges-Proskauer
A fully detailed poster schematic for this standard is available free for download from our website.
www.agtcbioproducts.com
Microbiology Media
AGTC Bioproducts
16
surfaces that are contaminated.
Processed Food Microorganisms can cause a variety of effects in food products including spoilage which primarily affects product quality, and food poisoning, which is generally caused by pathogens, . Bacteria can enter food products through different routes. In food processing, foodborne microbes can be introduced from infected humans who handle the food, or by cross contamination from some other raw material and/or the in-plant environment. For example, bacteria and viruses can be introduced by the unwashed hands of food handlers who are themselves infected.
In the kitchen, microbes can be transferred from one food to another by using the same knife, cutting board or other utensil to prepare both without washing the surface or utensil between. The way that food is handled after it is contaminated can also make a difference in whether or not, an outbreak occurs.
Product that is fully cooked can become cross contaminated if it comes into contact with raw material containing pathogens or with food contact
Culture media for Beer industry Beer is not a very appropriate medium for the development of bacteria due to its characteristics, such as the low quantity of available nutrients, the presence of alcohol, carbon dioxide and sulphur dioxide, as well as low conservation temperatures. Beer filtration and pasteurization phases also contribute to the stabilization of the product against microorganisms. The number of genera and species which usually contaminate it is limited. As is the case with wild yeasts, the contaminating bacteria cause turbidity and generate anomalous smells and bouquets. Lactic acid bacteria are the only Gram-positive ones that cause serious problems within beer-producing industries. The frequently found ones only belong to two genera: Lactobacillus and Pediococcus. Wild yeasts are also frequent contaminants in beer production.
In order to guarantee a process free of microorganisms, equipment and facilities should be kept in a perfect state of hygiene since contaminating elements almost always locate themselves in tanks, pipes, hoses, etc. It is also necessary to subject all equipment and raw materials including water, malt, triturates, hops, etc., to these controls.
Culture media specific for beer: Code
Description
AG-1438 NBB AGAR, MODIFIED AG-1440 NBB BROTH, MODIFIED
Selective medium used for the detection of Contaminating/spoilage micro-organisms in brewery
Size
Price
500 grams
£34.99
500 grams
£59.31
AG-1061 RAKA - RAY AGAR BASE
Selective medium to isolate lactic-acid bacteria in beer and beer fermentation processes
500 grams
£44.76
AG-1086 W.L. NUTRIENT AGAR
For the determination of microbial flora Cat : 1086 in beer fermentation processes and manufacturing
500 grams
£37.68
Phone: 01482 646020
Fax: 01482 646013
Email: office@agtcbioproducts.com
AGTC Bioproducts
17
Microbiology Media
We also supply bacteriological media for the following industrial sectors: Waste water. Cosmetics. Pharmaceutical products. Full details of all our 500 or more different products for bacteriological detection can be found on our web site, via our online catalogue at www.agtcbioproducts.com
www.agtcbioproducts.com
Synthesis Reagents
AGTC Bioproducts
18
Peptide Synthesis reagents and solvents These peptide synthesis reagents are manufactured in well equipped modern production facilities. All products are fully QC'd by thorough assay methodologies to check for chemical and chiral purity. All Solvents are fully QC’d for low levels of water, free amine and residue/particulates. Solvents For Synthesis Name
Cat. No
Acetonitrile 190 UV (HPLC) CAS Number: 75-05-8 EC Number: 200-835-2
AGMHP1008 -2.5lt
Dichloromethane EC Number: 200-838-9 CAS Number: 75-09-2 assay ≥99.8% (GC)
AGMBC7002 -2.5lt
Di-isopropylethylamine CAS Number: 108-18-9 assay ≥99.5%
AGBC7012 -1lt
Dimethylformamide CAS Number: 68-12-2 assay ≥98.0% (GC)
AGMHP1044 -2.5lt
DMF80%/Piperidine20% Premix
AGMPIPDMF -4lt
N-Methylpyrollidone AGMBC7006 -2.5lt
EC Number: 212-828-1 assay ≥99.5% (GC) PIPERIDINE CAS Number: 110-89-4 assay ≥99.5%
AGMPIP450 -450ml
size
Price
2.5 Ltr
P.O.A.
2.5 Ltr
£12.60
4 Ltr
£20.16
25 Ltr
£50.32
200 Ltr
£360.00
1 Ltr
£55.65
2.5 Ltr
£17.00
4 Ltr
£26.25
25 Ltr
£100.00
200 Ltr
£339.00
4 Ltr
£56.70
25 Ltr
P.O.A.
2.5 Ltr
£27.30
4 Ltr
£44.10
25 Ltr
£123.00
200 Ltr
£811.00
450ml
£22.05
Reagents & Linkers
Fmoc Amino Acids
(much more inside catalogue)
(Fmoc-D-Amino Acids & Boc (L & D) Amino Acids also available)
Cat. No
Name
5g
25g
100g
AGM01
Fmoc-L-Ala-OH
£6
£15
£43
Cat. No
Product
AG330645 HCTU
AGM02
Fmoc-L-Arg(Pbf)-OH
£40
£140
£423
AG94790
AGM03
Fmoc-L-Asn(Trt)-OH
£25
£59
£176
AG123333 HOBt . H2O
HBTU
AGM04
Fmoc-L-Asp(tBu)-OH
£25
£56
£166
AGX1060
AGM05
Fmoc-L-Cys(Trt)-OH
£25
£59
£176
AG148893 HATU
TBTU
100g
500g
1 Kg
£110
£331
£579
£91
£171
£228
£32
£107
£214
£60/50g
£72/100g
£317
AGM06
Fmoc-L-Gln(Trt)-OH
£25
£59
£176
AG39968
AGM07
Fmoc-L-Glu(tBu)-OH
£25
£56
£166
AGBC7012 DIPEA
AGM08
Fmoc-Gly-OH
£6
£15
£43
AGM09
Fmoc-L-His(Trt)-OH
£22
£43
£130
AGM10
Fmoc-L-Ile-OH
£6
£15
£41
AGM11
Fmoc-L-Leu-OH
£6
£15
£41
Cat.No
5g
HOAt
£165/25g £55.65/Litre
Phospho Fmoc Amino Acids Product
Size Price
AGM12
Fmoc-L-Lys(Boc)-OH
£20
£43
£130
AGM16.3 Fmoc-L-Ser(PO(OBzl)OH)-OH
AGM13
Fmoc-L-Met-OH
£6
£15
£41
AGM16.3 Fmoc-L-Ser(PO(OBzl)OH)-OH
10g
£391 £743
£41
AGM17.2 Fmoc-L-Thr(PO(OBzl)OH)-OH
5g
£430
AGM14
Fmoc-L-Phe-OH
£6
£15
AGM15
Fmoc-L-Pro-OH
£6
£15
£41
AGM17.2 Fmoc-L-Thr(PO(OBzl)OH)-OH
10g
£818
AGM16
Fmoc-L-Ser(tBu)-OH
£25
£46
£140
AGM19.4 Fmoc-L-Tyr(PO(OBzl)OH)-OH
5g
£484
AGM17
Fmoc-L-Thr(tBu)-OH
£25
£46
£140
AGM19.4 Fmoc-L-Tyr(PO(OBzl)OH)-OH
10g
£920
AGM18
Fmoc-L-Trp(Boc)-OH
£31
£94
£283
AGM19
Fmoc-L-Tyr(tBu)-OH
£25
£43
£130
AGM20
Fmoc-L-Val-OH
£6
£15
£41
Phone: 01482 646020
Fax: 01482 646013
Email: office@agtcbioproducts.com
Transfection
AGTC Bioproducts
19
Ÿ High-Efficiency-Transfection Reagent Low Toxicity Simple Procedure Ÿ Low Cost HiTranTM is a Polylipid-based reagent designed and formulated with proprietary technology for transfecting DNA or siRNA into a variety of eukaryotic cell lines, including HEK293, CHO, COS, NIH3T3, MCF-7, PC12, RKO, C2C12 myoblasts, mouse embryonic stem cells (ES) and fibroblasts (MEF), mesenchymal stem cells, NCCIT, S2
(Schneider) Drosophila cells as well as primary cultures of human carcinoma, rat adrenal chromaffin cells, mouse and rat cortical neurons, hepatocytes, etc. HiTranTM also offers extremely high transfection efficiencies and minimal cytotoxicity for suspension cultures including HEK 293, mouse lymphocytes, and insect cells (sf9, sf21, High-Five). The reagent works very well in cultures with serum concentration range from 0 to 20% FBS. GFP expression in different cell lines:
CO
PC1
H E K
C2C1
Transfection of HEK293 cells with triple lentivirus vectors using HiTran and R2000: HiTran 60µl/30µg vectors/per 15 cm plate DAPI
GFP
combined
R2000 60µl/30µg vectors/per 15 cm plate DAPI
Transfection Reagents
GFP
combined
Description
Size
AGTC-HT-1211-01 AGTC-HT-1211-02 AGTC-HT-1211-03
Price
0.5mL £93.75 1.0mL £175.00
High Efficiency Transfection Reagent
1.5mL £242.50
AGTC-HT-1211-04
6.0mL £867.50
AGTC-HT-1212-01
0.5mL £100.00
AGTC-HT-1212-02 AGTC-HT-1212-03
Transfection Reagent for Transient Protein Expression
AGTC-HT-1212-04
Phone: 01482 646020
1.0mL £187.50 1.5mL £262.50 6.0mL £937.50
Fax: 01482 646013
Email: office@agtcbioproducts.com
Plasmid Purification Ÿ Ÿ Ÿ Ÿ
20
Superior Purity Ultra High Yield Time Saving Eco-Friendly and Low Cost
"A Breakthrough Plasmid DNA Purification System" Superior Purity: This patented Plasmid Purification system uses no chaptropic salts in the buffer, and the resulting purified DNA is free of guanidine salts, caesium chloride, ethidium bromide and ion exchange resin residues, which enable the highest performance of downstream applications including gene therapy and genetic vaccination.
our methodology produces higher DNA yield while requiring fewer bacterial cultures. Time Saving: Using our unique Megaprep Kit, 10 mg Plasmid DNA can be purified from 1,000 mL culture within 1 hour! Environment benefit: The Quickgene System is the only method available that does not utilize Guanidine Salt, a hazardous protein denaturant, in the silica based purification system, which will ensure the safety and health of scientists and the environment!
Ultra High Yield: When comparing our' Plasmid Purification System to other purification methods, KIt
Description
Plasmid Miniprep
Easy and reliable methods to purify plasmid DNA from 1 mL to 12 mL bacterial cultures in less than 20 minutes. The DNA is ready for downstream applications such as restriction digestions, PCR, and automated sequencing. Ÿ Kit I: 10‐20 μg of high copy number plasmid DNA can be isolated from 1‐2 mL culture. Kit II: 30‐80 μg from 5-12 mL culture.
Plasmid Midiprep
This kit provides an easy and reliable method to purify plasmid DNA from 50-100 mL bacterial cultures in less than 60 minutes. The DNA is ready for downstream applications such as transfection, RFLP, DNA amplification, and automated sequencing. Ÿ Kit I: 100‐200 μg of high copy number plasmid DNA can be isolated from 50 mL culture. Kit II: 200‐400 μg from 100 mL culture. Ÿ Choice of 2 types of columns for centrifugation or vacuum manifold. Endofree Midiprep: Endotoxin < 0.2 EU / μg DNA.
Plasmid Maxiprep
This kit provides an easy and reliable method to purify plasmid DNA from 150 to 250 mL bacterial cultures in less than 60 minutes. The DNA is ready for downstream applications such as transfection, RFLP, DNA amplification, and automated sequencing. Ÿ 1000‐1500 μg of high copy number plasmid DNA can be isolated from 200 mL culture.per column Ÿ Choice of 2 types of columns for centrifugation or vacuum manifold. Ÿ Endofree Maxiprep: Endotoxin < 0.2 EU / μg DNA.
Plasmid Megaprep
Based on our proprietary technology and unique design, milligrams of high quality DNA can be purified within an hour. The purified DNA is ready for downstream applications such as transfection, RFLP, DNA amplification, and automated sequencing. Ÿ 2-layer filter unit. Ÿ No centrifugation in the entire procedure. Ÿ Megaprep 3/6: 3 - 6 mg of high copy plasmid DNA can be purified from 1000 mL culture in 60 minutes. Ÿ Megaprep12: up to 12 mg of high copy plasmid DNA can purify from 2000 mL culture in 60 minutes. Ÿ Endofree Megaprep: Endotoxin < 0.2 EU / μg DNA. Ÿ The endofree Mega kits are especially suitable for DNA vaccination and gene therapy applications.
96-well Plasmid Miniprep
This high-through-put system is designed for the parallel preparation of 96 plasmid minipreps within 1 hour. The purified DNA is ready for downstream applications such as DNA amplification, automated sequence, RFLP, transfection, and viral packaging. Ÿ Fast and reliable. Ÿ 96 DNA preps can be done in less than 40 minutes. Ÿ One-step filter-binding, which omits lysate transferring from plate to plate. Ÿ 8‐12 μg DNA per well for high copy plasmid from 1.5 mL culture. Ÿ Can be adapted to many automated platforms
Phone: 01482 646020
Fax: 01482 646013
Email: office@agtcbioproducts.com
21 Code
Plasmid Purification
Description
AG-PD1211
Plasmid Miniprep Kit I : 10‐20 μg of high copy number plasmid DNA can be isolated from 1‐2 mL culture
AG-A26PD1213
Plasmid Miniprep Kit II:Kit II: 30‐80 μg of high copy number plasmid DNA can be isolated from from 5‐12 mL culture.
AG-PD1411
Plasmid Midi Kit: 100‐200 μg of high copy number plasmid DNA can be isolated from 50 mL culture
AG-PD1412 AG-PD1413
Plasmid Midi Kit II 200‐400 μg of high copy number plasmid DNA can be isolated from 100 mL culture. Plasmid ezFilter Midi Kit I
AG-PD1414
Plasmid ezFilter Midi Kit II
AG-PD1511
Plasmid Maxi Kit
AG-PD1512
Plasmid ezFilter Maxi Kit
AG-PD1611 AG-PD1612
Plasmid ezFilter Mega3 Kit Plasmid ezFilter Mega6 Kit
AG-PD1614
Plasmid ezFilter Mega10 Kit
AG-PD1811
96-well Plasmid Mini Kit
AG-PD1812
96-well Plasmid ezFilter Mini Kit
AG-PD1212
AG-PD1214 AG-PD1415
EndoFree Plasmid Mini Kit I EndoFree Plasmid Mini Kit II EndoFree Plasmid Midi Kit
AG-PD1416
EndoFree Plasmid ezFilter Midi Kit
AG-PD1514
EndoFree Plasmid Maxi Kit
AG-PD1515
EndoFree Plasmid ezFilter Maxi Kit
AG-PD1615 AG-PD1616
EndoFree Plasmid ezFilter Mega3 Kit EndoFree Plasmid ezFilter Mega6 Kit
AG-PD1617
EndoFree Plasmid ezFilter Mega10 Kit
AG-D1711
HP Plasmid Mini Kit
AG-D1712
HP Plasmid Midi Kit
AG-D1713 AG-D1714
HP Plasmid Maxi Kit HP Plasmid ezFilter Mega3 Kit
www.agtcbioproducts.com
No :
Price
50
£28.1
250
£137.5
50
£37.5
250
£156.3
10
£32.5
25
£78.1
10
£49.4
25
£109.4
10
£49.4
25
£109.4
10
£55.6
25
£121.9
10
£75.0
25
£156.3
10
£81.3
25
£175.0
2
£50.0
10
£237.5
2
£62.5
10
£287.5
2
£87.5
10
£425.0
4x96
£250.0
20x96
£1,250.0
4x96
£312.5
20x96
£1,437.5
50
£36.3
250
£156.3
50
£42.5
250
£181.3
10
£49.4
25
£109.4
10
£56.3
25
£125.0
10
£87.5
25
£175.0
10
£93.8
25
£187.5
2
£55.0
10
£250.0
2
£68.8
10
£312.5
2
£93.8
10
£450.0
50
£37.5
250
£162.5
10
£43.1
25
£100.0
10
£100.0
25
£237.5
2
£56.3
10
£250.0
Dithiothreitol
AGTC Bioproducts
22
Dithiothreitol (DTT) Cleland's Reagent DTT is an unusually strong reducing agent, used extensively in organic reactions. Our DTT is produced to the highest standards possible (see attached technical specification) and with additionally extremely low levels of Bromine and Lead. The synthesis we use produces a particularly odour reduced product making it highly suitable for commercial kit manufacture. Some applications are: As a reducing or "deprotecting" agent for thiolated DNA. Reduction of disulfide bonds in proteins. Prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. DTT can also be used as an oxidizing agent. For larger sizes 100g-to kilo’s call us today to receive a free evaluation sample and a competitive quote:
CAS number
other names (2S,3S)-1,4-dimercaptobutane-2,3-diol
3483-12-3
Properties
Threo-1,4-dimercaptobutane-2,3-diol
Molecular formula
C4H10O2S2
DL-Threo-1,4-dimercapto-2,3-butanediol
Molar mass Appearance Melting point Boiling point Solubility in water
154.25 g mol White solid 42-43 °C 125-130 °C at 2 mmHg Soluble
1,4-Dithio-DL-threitol ±-Threo-1,4-dimercapto-2,3-butanediol Cleland's reagent Reductacryl
A common use of DTT is as a reducing or "deprotecting" agent for thiolated DNA. The terminal sulphur atoms of thiolated DNA have a tendency to form dimers in solution, especially in the presence of oxygen. Dimerization greatly lowers the efficiency of subsequent coupling reactions such as DNA immobilization on gold in biosensors. Typically DTT is mixed with a DNA solution and allowed to react, and then is removed by filtration (for the solid catalyst) or by chromatography (for the liquid form). The DTT removal procedure is often called "desalting." DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. However, even DTT cannot reduce buried (solvent-inaccessible) disulfide bonds, so Cat No
reduction of disulfide bonds is sometimes carried out under denaturing conditions (e.g., at high temperatures, or in the presence of a strong denaturant such as 6 M guanidinium hydrochloride, 8 M urea, or 1% sodium dodecylsulfate). Conversely, the solvent exposure of different disulfide bonds can be assayed by their rate of reduction in the presence of DTT. DTT can also be used as an oxidizing agent. Its principal advantage is that effectively no mixeddisulfide species are populated, in contrast to other agents such as glutathione. In very rare cases, a DTT adduct may be formed, i.e., the two sulfur atoms of DTT may form disulfide bonds to different sulfur atoms; in such cases, DTT cannot cyclize since it has no remaining free thiols.
Name
EC-601-25gm Dithiothreitol DTT, >98% purity, low odour EC-601-50g Dithiothreitol DTT, >98% purity, low odour
Price £49 £89
23 Terms & Conditions (2010) In these Conditions the 'Seller' means AGTC Bio products Limited, 'the Buyer' means the person, firm, institute or Company purchasing the goods. 'the Goods' means the goods or materials which shall be the subject of the contract between the Seller and the Buyer 'the Price' means the price specified in the Sellers Quotation, Acknowledgment or otherwise communicated to the Buyer and agreed. SPECIFICATIONS Specifications and detail in catalogues and leaflets are subject to amendment without notification. The right is reserved by the Seller to supply goods to the specification and/or design current at the date of dispatch. CATALOGUES, DRAWINGS, SKETCHES All statements (whether written or oral, descriptions, drawings sketches, photographs, illustrations, diagrams or specifications concerning the goods made or given by or for the Seller before contract, whether in catalogues, brochures, leaflets, price lists or otherwise, are for the purpose of information and guidance only. Where goods are sold by reference to descriptions in a catalogue, the goods are sold subject to the tolerances and variations expressed or implied in the catalogue. In that design and sources of supply may change from time to time the Seller shall have the right to supply goods of altered design or from the new source of supply. PRICES All goods will be supplied at the price and the Purchase Tax rate ruling on the date of dispatch. All prices shown in catalogues, leaflets or brochures, are net ex works prices exclusive of (Purchase Tax) and delivery charges, and are for guidance only. The Seller reserves the right to vary such prices at any time in its discretion. Quotations expressed to be for a fixed price remains open for acceptance by the Purchaser within the period stated in the quotation or if no period stated, within 30 days of the date of the quotation irrespective of when the quotation is received by the Purchaser. ACCEPTANCE OF ORDER All orders are accepted by the Seller subject to these Conditions of Sale unless otherwise varied by agreement in writing. Contracts for delivery by installments shall be deemed to be indivisible. THE PRICE 1. The Seller may at any time before delivery increase the price of the undelivered balance of the goods by notice in writing to the Buyer. In such event, the Buyer may, within seven days of receipt of such notice by notice in writing, cancel the order so far as the undelivered balance of the Goods concerned. 2. Unless otherwise stated on acceptance, a nominal charge of £13 will be added to the price of the Goods to cover shipment and insurance within the UK for consignments up to 20 kilo's and 50p per kilo there after (contract pricing is available for bulk consignments). Outside of the UK the following shipment charges will apply Europe £30 will be added USA and rest of world 40 will be added all include the Sellers costs of standard packing, normal insurance and delivery of the Goods to any one address in the United Kingdom specified in writing by the Buyer to and agreed by the Seller prior to delivery. 3. Where an order is placed for goods having a total invoiced value (excluding Value Added Tax and any additional transport, insurance, packing and/or additional costs) of less than £50 the Seller reserves the right to make an additional charge of £20. 4. Containers and/or packages are non-refundable unless stated in the Sellers Quotation, Acknowledgment, or other documentation. Returnable containers and/or packages shall be returned empty by the Buyer to the Sellers address stated on the delivery note in good condition, carriage paid, within three months of delivery of the Goods. If not, such containers and/or packages will be chargeable at replacement value and no credit will be due on containers and/or packages for which a charge shall have been made by the Seller. PAYMENT 1. Payment shall be made at time of ordering by bank credit card or bank debit card, unless account payment has been agreed in advance of ordering. 2. Where account payment has been agreed and unless otherwise stated on the Sellers invoice or otherwise agreed in writing, payment for the goods shall be made not later than thirty days after the end of the month of invoicing but so that the Seller may at any time on or after acceptance by notice in writing to the Buyer vary the terms of payment by demanding immediate payment or (at the Sellers option) adequate security for sums which will be due hereunder 3. Time of payment shall be of the essence and failure by the Buyer to pay the price or any installment thereof in due time shall entitle the Seller to treat such failure as a repudiation of the whole Contract by the buyer and to require the Buyer to make immediate payment of all monies due or to become due and to recover from the Buyer damages for such breach of contract and/or (at the Sellers option) to charge interest at either five percent per month or two percent above the Base Rate of LloydsTSB Bank Ltd. whichever shall be the greater, from the due date until payment.. DELIVERY Unless otherwise agreed in writing between the Seller and the Buyer the following provisions shall apply. I. Delivery of the Goods shall take place when the Goods shall be delivered at the address specified on the Sellers Quotation, Acknowledgment or other documentation. Were ever possible delivery will be next day or within two days of receipt of the order. If such delivery is not possible the consignment will be delivered at the earliest practicable moment. II. The delivery date or dates specified on the Sellers acceptance of order are estimates only The Seller shall not be liable for failure to deliver by such date or dates or for any damage or loss arising directly or indirectly out of delay in delivery, nor shall the Buyer be entitled to refuse to accept the Goods because of late delivery. iii. The risk of any loss or damage to or deterioration of the Goods shall be borne by the Buyer from the time delivery has taken place as in QI above. III. In respect of Sea Transit the Seller shall not be required to give the Buyer the notice relating to Insurance of the Goods referred to in Section 32(3) of the Sale of Goods Act 1979. v. The Seller reserves the right to designate minimum order quantities and/or minimum handling charges for any products. PROPERTY I. Notwithstanding delivery and the passing of risk and solely for the purposes of securing payment of all monies due or to become due to the Seller by the Buyer on any account in the event of the Buyer entering liquidation or having a winding up order made against it or a receiver or receiver and manager being appointed of its assets or income or any part thereof or in the event of the Buyer being an individual or individuals he or either of them committing any act of bankruptcy or having any bankruptcy petition presented against him or either of them: I I. The property in the Goods shall remain in the Seller until payment in full has been made to the Seller by the Buyer for the Goods and all other sums due to the Seller at the date of delivery of the Goods. III. Where full payment has not been made to the Seller and the Buyer uses the Goods in his manufacturing process or incorporates the goods with other products the property in the Goods shall be retained by the Seller in so tar as such Goods are identifiable. IV. Until such payment is made the Buyer shall hold all Goods and materials the property in which is vested in the Seller on a fiduciary basis only and in any of the events specified above the Buyer shall store such Goods and materials so as to be clearly identifiable as the property of the Seller. LIEN The Seller shall in respect of all unpaid debts due from the Buyer under the same or any other contract have a general lien on all goods and property of the Buyer in its possession (although such goods or some of them may have been paid for) and shall after the expiration of fourteen days written notice to the Buyer be entitled to dispose of such Goods and property as it deems fit and apply the proceeds towards such debts. SHORTAGES AND DAMAGES and/or LOSS IN TRANSIT 1. Unless otherwise agreed in writing between the Buyer and Seller the Seller may deliver against any order an excess and/or deficiency up to ten per cent of weight or volume ordered without any liability whatsoever to the Buyer save that the price shall be adjusted accordingly.
24 2. The Buyer shall inspect the goods immediately on delivery and shall within three days of such delivery (time being of the essence) give notice in writing to the seller and the carrier of all claims on account of damage to or total or partial loss of Goods in transit Claims for non-delivery must be submitted in writing to the seller within fourteen days after notification of dispatch. Quality claims must be made in writing 3. Immediately after the Buyer learns of the defect and in any event not later than sixty days after the Buyer's receipts of the Goods. Any claim not made in writing and received by the Seller within the aforesaid time limits shall be deemed waived If the Seller fails to make delivery or makes defective delivery of any one installment such failure or defective delivery shall not vitiate the contract as regards other installments. 4. The right of the Buyer to set off the value of any shortage, defective Goods or Goods not otherwise conforming to contract shall be restricted to the specific invoice for the Goods in question and shall not apply to previous or future accounts. 5. Subject to the criteria above all Refunds and credits for damaged and defective goods will be issued within 60 days after formal acceptance by the seller in writing of the buyers complaint. WARRANTY AND LIMITATION OF LIABILITY The Seller warrants that the Goods are produced within the accepted tolerance level in accordance with the standard specifications stated in the Sellers official literature on the Goods current at the relevant time. Save as aforesaid, all other conditions, guarantees or warranties whether expressed or implied by statute, common-law or otherwise including (0ut without prejudice to the generality of the foregoing) conditions, guarantees or warranties as to quality, fitness for purpose or description of the Goods or their life or wear or use under any conditions whether known or made known to the Seller or not are hereby excluded. The Sellers liability for any and all direct loss or damage resulting to the Buyer from defects in the Goods or any other cause shall be limited to the purchase price of the quantity of the Goods in respect of or in relation to which such loss or damage is claimed. Subject as aforesaid the Seller shall be under no liability in contract or in tort fur any loss or damage or personal injury resulting directly or indirectly out of the supply or use of the Goods or containers other than death or personal injury resulting from the negligence of the Seller within the meaning of Section u of the Unfair Contract Terms Act 1977. FORCE MAJEURE Deliveries may be partially or totally suspended by either party during any period in which it is prevented from manufacturing, delivering or taking delivery of the Goods through any circumstances outside its control if, because of such circumstances, the Seller is unable to supply the total requirement of the Goods the Seller may allocate its available supply (after satisfaction of its own requirements) among all of its customers, including those not under contract as the Seller thinks fit Deliveries so suspended shall be cancelled without liability, but the contract between the parties shall otherwise remain unaffected. INDEMNITY The Buyer shall indemnity the Seller in respect of all damage or injury occurring to any person, firm company or property and against all actions, suits, claims and demands, charges or expenses in connection therewith for which the Seller may become liable in respect of the Goods sold under the contract in the event that the damage or injury shall have been occasioned otherwise than by the negligence of the Seller. DEFAULT The Seller reserves the right (without prejudice to its other rights and remedies) either to terminate the contract between the parties or to suspend further deliveries under it or require payment in advance in the event that the Buyer fails to pay for any one delivery when the same becomes due or the Buyers financial responsibility becomes unsatisfactory to the Seller or if the Buyer, being a company goes into liquidation or has a receiver appointed or not being a company has a receiving order made against him or enters into any arrangement or composition with creditors. DUTY All import duties, VAT and other imposts will be for the Buyer's account. PATENTS AND TRADEMARKS No representation, warranty or indemnity is given by the Seller that the Goods do not infringe any letters Patent, trade marks, registered designs or other industrial rights. ASSIGNABILITY The contract of which these Conditions form part is personal to the Buyer who shall not assign the benefit thereof without the Sellers consent PROPER LAW These conditions and the contract between the parties shall be construed and applied in accordance with the Law of England and the English Courts shall have sole jurisdiction in any dispute relating thereto. Copyright Š AGTC Bioproducts ltd 2009
GLOBAL LIFE SCIENCE SUPPLY
Contact Us: AGTC Bioproducts t/a National Diagnostics Tel: 01482 646020 Fax: 01482 646013 Email: office@agtcbioproducts.com www.agtcbioproducts.com Unit 4 Fleet Business Park, Itlings Lane, Hessle, Hull, HU13 9LX