Catarina Duarte Directora Unidade de Nutracêuticos
Fórum Estratégico de Oeiras Valley 25 de Outubro - 9h30
Universidade Nova de Lisboa (UNL) Instituto de Tecnologia Química e Biológica (ITQB)
Faculdade de Ciências e Tecnologia (FCT)
Instituto Gulbenkian de Ciência (IGC)
16 Portuguese Companies as Partners:
Pharmaceutical/ Health Sciences Agro-forestry, pulp and paper Agroindustry Oeiras
Campus of ITQB, IGC, IBET (Lab. Associado) 30.000m2, 800 scientists and researchers of which 200 Ph.D.’s. Over
70 personnel directly paid by IBET
16 x 75m2 fully equiped laboratories
2.400 m2 Pilot Plant plus cGMP GenIBET (45%IBET)
Human resources: 20 PhD 42 graduate researchers 5 Technicians 8 administratives
• Contract Research Projects • “Umbrella Research Contracts” plus per project based contracts
• Joint Research for Advanced Training (e.g. PhD Students, technicians publications submitted after both parties approval)
FLEXIBILITY & CONFIDENTIALITY SPECIFIC NEEDS
iBET’s Mission
To foster the competitiveness of our customers and partners, by creating wealth out of knowledge of Chemistry, Biochemistry and Biology
Range of Services @ iBET
Animal Cell Technology Bioprocess Development for: Recombinant proteins DNA, VLP’s, retroviral, lentiviral and adenoviral vectors
Diagnostics and vaccine candidates for AIDS, Rotavirus, Leishmania, Peste des petits ruminants
Alternative model systems as tools for biochemical and pharmacological studies, Cell handling based on surface controlled differentiation, 3D culture systems, primary cultures and stem cells – hESC, adult stem cells (e.g. MSC), iPS Bottom up systems biotechnology, Scaled down tools for DSP.
Animal Cell Technology • Cell Bioprocesses Lab headed by Paula Alves
• Engineering Cellular Applications Lab headed by Manuel Carrondo
• Cell Line Development and Molecular Biology Lab, headed by Ana Sofia Coroadinha.
ACT TEAM 10 senior researchers and post-docs 4 technicians (Bioreaction, dowstream and analytics, lab manager 18 PhD students plus 4 MSc Students 4 research assistants
Pilot Plant Track-record Biopharmaceutical Products Supercritical Fluid Extraction
Vaccine Process Development Diagnostics Supply of purified proteins for structure function (Chrystallographic and Proteomic)
Centrifugation
Micro / Ultra filtration
Fermenters up to 400L 32 port MS for off gas analyses Centrifuges (disc and solid bowl) High pressure homogenizers (1200 bars) Micro/ultra filtration unit Freezedryer (1,5 m²) Thin-film evaporator (40L/h flow rate) Rotavapor (50L) Crystallizer, settler and single-layer process filter (120L) Liquid-liquid extractor (2 stage counter current cross flow) Preparative and pilot scale chromatography UBICON supervisor and process control system Supercritical fluid extraction units (for solid and liquid extraction)
Cell disruption
Main Equipment
Purification
Pilot Plant
Process development
CLIENT
Process scale-up
Quality Control
Viral clearance
GMP Manufacturing
Cell line development and optimization Cell banking and storage
Assay development and validation
Facilities A cGMP Bridge to Personalized Medicine
Virus Unit Bacterial Unit QC Labs
Fill and Finish
Cell Culture
www.genibet.com
Proteomics
3D protein structure determination (X-Ray, NMR) Limited Proteolysis for protein construct design
Differential Fluorimetry Assays (Thermofluor) for protein stability studies Molecular modeling of protein structures, protein-ligand and protein-protein interactions
Proteomics
Current Activities Collaboration with Bayer Pharmaceuticals Collaboration with Merck Serono
Track record Protein Chemistry
Molecular Biology
Structural Biology
Molecular Modelling/ Structural Analysis
Bioinorganic Chemistry characterization
Thermodynamics and kinetics of Redox and metalloproteins
Analytical Services Unit Joint department of IBET and ITQB under the executive management of IBET Analytical development, validation and testing of chemicals and biologicals as well as studies on candidate pharmaceutical products according to OECD Good Laboratory Practices Principles The Unit is certified by the INFARMED and IPQ as compliant with GLP.
Analytical Services Unit Analytical Laboratory Chromatographic (HPLC and GC with several detectors) and electrophoretic methods for pharmaceutical, agro and chemical industry and academia.
Cell Based Assays and Microbiology Laboratory in vitro potency assays, protein quantification, molecular biology analysis (GMOs in food and feed and other) and detection and quantification of impurities or contaminants in pharmaceutical. Development of Cell Based Assays.
Mass Spectrometry Laboratory Development and validation of analytical methods as well as routine analysis for a broad range of chemical compounds, from small organic and organometalic compounds to peptides, oligosaccharides, nucleotides and proteins.
Nutraceuticals and Delivery main activities : •Green Processes for developing health promoting products •Supercritical Fluid Technology – application to extraction and precipitation processes •Materials Processing – particle formation, hybrid structures and microbubbles, nano/micro encapsulation and impregnation
projects include: •Development of Drug Delivery Systems •Functional Products and Nutraceuticals Formulation •Health claims assessment (in-vitro bioactivity evaluation
Production of natural extracts/Isolation of bioactive ingredients
Nutraceuticals and Delivery
High pressure and supercritical fluid technology
Conventional Extraction (with biocompatible solvents)
Green sustainable processes (that reduce or eliminate the use or generation of hazardous substances
Membrane Filtration Bioactive rich fractions (e.g. polyphenols, carotenoids, phytosterols, terpenes,...)
Adsorption process (with macroporous resins)
Natural colours Natural flavours
Natural preservatives
Nutraceuticals and Controlled Delivery Supercritical Fluid Technologies
Increase Shelf
“Conventional� Precipitation Techniques
life and stability of the
bioactive Increase the
Increase
bioactive
Formulation/ Encapsulation
efficacy
solubility
Protect
Increase
against GIT
bioavailaibity
23
Biomathematics Glicobiology
Supercritical Fluids Chiral Synthesys Ionic Liquids
Contract, project, resources are time limited
Interface
Projects With Foreign Companies
Discovery
Preclinical
Biologicals & ATMP’s Mab’s Bayer Köln (Sat. Lab) Micromet Munich
StructuralBiology/ Proteomics Bayer Berlin MS Darmstadt
Primary & Stem Cells CEVEC Köln Merck & Co., Rahway, NJ Astrazeneca, Göteburg Centocor, J&J NJ Cellon, Luxembourg
Bayer, Wuppertal MS Darmstadt, Geneve Merck & Co., West Point, PA Merck & Co., Rome (IRBM) Genethon, Paris Apceth, München Alloksys, Bunnik (NL) Sanofi Pasteur, Lyon
Engineering Merck & Co., West Point, PA GlicoFi, New Hampshire Lonza, UK Novartis, Switzerland Leti, Spain Sartorius-Stedim, Göttingen
Plant Biotech – BASF, Bayer Crop Sciences