DNA Polymerases for PCR success by AllScience LLC

PCR Polymerases High yield. High fidelity. Lyophilized. Long PCR. GC-Rich. Direct PCR. Fast PCR. Standard PCR.

1842

1925

1950

1979

Predecessor to Takara Bio begins as a small sake brewery business

Formally incorporates as Takara Shuzo Co., Ltd

Takara Bio founds a biotechnology R&D center

Commences sales of the first domestically produced restriction enzymes in Japan

DNA Polymerases for PCR success

Powerful enzymes deliver powerful results. Best-in-class enzymes backed by expert scientific support We create polymerases that simply work harder, better, and faster— so you can focus on pushing your experiments to their full potential.

Whether you’re performing next-generation sequencing, cloning, genotyping, or using other technologies, the successful amplification of DNA is key. We understand how critical it is for your PCR polymerases to perform reliably for every single reaction, and that your time and resources are limiting. Each sample and every decision matters—because every result counts. Your success is what drives our scientists to develop the highestperforming enzymes. Takara Bio has over 90 years of experience in pursuing excellence in biochemistry tools, beginning with the improvement of fermentation techniques for cultivating microbes. In 1950, we launched a biotech R&D center that has yielded cuttingedge advances. This established practice of quality and progress continues today—our extensive portfolio of PCR polymerases supports a wide variety of applications, from basic research to routine laboratory testing, for laboratories around the world. The technical support you receive from Takara Bio comes from decades of experience in fine-tuning enzyme performance. High performance, value, and expert technical support are the reasons that scientists worldwide have published tens of thousands of peer-reviewed studies using PCR polymerases from Takara Bio. We create polymerases that simply work harder, better, and faster— so you can focus on obtaining the results you need.

1984

1988

1993

2005

Clontech Laboratories, Inc. is established

Acquires an exclusive distribution right in Japan for a gene amplification system using PCR technology

Commences sales of Takara branded PCR products

Acquires Clontech Laboratories, Inc. of the United States

2017 Acquires Rubicon Genomics

...whatever your needs. High Yield for low-copy targets

Standard PCR

page 4

for cloning, mutagenesis

for PCR assays page 11

Fast PCR

page 5

PCR

for rapid screening page 10

Lyophilized for field testing page 6

Long PCR

Direct PCR

for HLA Typing

from tissue page 9

High Fidelity

GC-Rich

page 7

for NGS library prep page 8

Need commercial or custom products? We offer royalty-free DNA polymerase formulations, glycerol-free enzymes for lyophilization, OEM and customization services, such as custom packaging and product configurations. Please inquire atÂ bd_oem@takarabio.com.

takarabio.com

High-Yield Polymerases Some PCR applications require amplification of targets present at low copy, or even a single copy. In other situations, samples may be so limiting or precious that PCR failure is simply not an option. When yield is critical, we recommend the following polymerases.

Mammalian Genotyping

Library Construction

Routine PCR

Low Copy Number and/or Dirty Samples

Titanium® Taq

Advantage® 2

EmeraldAmp® MAX HS PCR Master Mix

TaKaRa Ex Taq® DNA Polymerase

Sensitive and robust

Long PCR and enhanced fidelity

Streamlined workflow

Higher fidelity and yield

gDNA

<2 kb

<6 kb

<15 kb

<20 kb

Plasmid/lambda

<2 kb

<18.5 kb

<30 kb

cDNA

<4 kb

<8.5 kb

Yes

T/A

Yes

Available

Yes

Available

Yes

Available

Application

Recommended product Choose for: Amplicon size

Enzyme properties 5'–3' exonuclease activity 3'–5' exonuclease activity T/A overhangs or blunt

T/A

Format/features Premix Eco-friendly lyophilized format PCR kit with control template and primers available

Available

Yes

Dye added Hot start

Yes

P R O D U C T S Cat. #

Product

Size

639208

Titanium Taq DNA Polymerase

100 Rxns

639201

Advantage 2 Polymerase Mix

100 Rxns

RR330A

EmeraldAmp MAX HS PCR Master Mix

160 Rxns

RR001A

TaKaRa Ex Taq DNA Polymerase

250 Units

Additional sizes available. Check product page at takarabio.com for more information.

High-Fidelity Polymerases Cloning and expression, sequencing, structure-function studies, and genome variation are a few reasons to reach for a high-fidelity PCR polymerase. But not all high-fidelity enzymes are created equal. Many offered by other manufacturers lack the speed or processivity needed for demanding high-fidelity long PCR, fast PCR, or amplification of GC-rich or repetitive targets. Choose from these polymerases for versatile performance whenever accuracy is required. Application

In-Fusion® Cloning

Long PCR, Sequencing, Cloning

Cloning and Expression, DNA Sequencing