Query Query
Results
Date
12 substances in Reaxys
2018-04-14 18h:18m:53s (UTC)
O N
O N
1. Query
N F O
Search as: As drawn
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Reaxys ID 26138911 View in Reaxys
1/12
H 11 3 C
CAS Registry Number: 74143-28-5 Linear Structure Formula: C15 (11)CH12FN3O3 Molecular Formula: C16H12FN3O3 Molecular Weight: 312.277 InChI Key: PPTYJKAXVCCBDU-BJUDXGSMSA-N Note:
O
N O
N
N F
O
Druglikeness (1) 1 of 1
LogP
2.349
H Bond Donors
0
H Bond Acceptors
3
Rotatable Bonds
2
TPSA
78.49
Lipinski Number
4
Veber Number
2
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dissociation constant of the compound (11C labeled) towards gamma-aminobutyric acid A-Benzodiazepine receptor measured at 0 degree C
Substance RN
26138911View in Reaxys
Measurement Parameter
Kd
Unit
nM
Qualitative value
=
Quantitative value
1
Measurement pX
9
Medchem (1) 1 of 1
Reaxys ID 702691 View in Reaxys
2/12 CAS Registry Number: 1622-62-4 Chemical Name: flunitrazepam; 1-methyl-5-(2'-fluoro-phenyl)-7-nitro-1,3-dihydro-2H-1,4-benzodiazepin-2-one; Flunitrazepam; 5-(2fluorophenyl)-1,3-dihydro-1-methyl-7-nitro-2H-1,4-benzodiazepin-2one; (+/-)-flunitrazepam; [3H]-flunitrazepam; 3H-flunitrazepam Linear Structure Formula: C16H12FN3O3 Molecular Formula: C16H12FN3O3 Molecular Weight: 313.288 Type of Substance: heterocyclic InChI Key: PPTYJKAXVCCBDU-UHFFFAOYSA-N Note:
O N O
N
N F
O
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Substance Label (19) Label References 235
Oda, Shingo; Fujiwara, Ryoichi; Kutsuno, Yuki; Fukami, Tatsuki; Itoh, Tomoo; Yokoi, Tsuyoshi; Nakajima, Miki; Drug Metabolism and Disposition; vol. 43; nb. 6; (2015); p. 812 - 818, View in Reaxys
G55
Sobaska, Anna W.; Zydek, Grazyna; Wlodno, Piotr; Brzeziska, Elzbieta; European Journal of Medicinal Chemistry; vol. 89; (2014); p. 147 - 155, View in Reaxys
FMN
Megarbane, Bruno; Pirnay, Stephane; Borron, Stephen W.; Trout, Herve; Monier, Claire; Risede, Patricia; Boschi, Gabrielle; Baud, Frederic J.; Toxicology Letters; vol. 157; nb. 3; (2005); p. 211 - 219, View in Reaxys
1
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys
Merck 4181
Giorgi; Lecca; Cancedda; Serra; Corda; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 290; nb. 1; (1995); p. 37 - 47, View in Reaxys; Klein; Mascia; Harkness; Hadingham; Whiting; Harris; Journal of Pharmacology and Experimental Therapeutics; vol. 274; nb. 3; (1995); p. 1484 - 1492, View in Reaxys; Duncalfe; Dunn; European Journal of Pharmacology; vol. 298; nb. 3; (1996); p. 313 - 319, View in Reaxys; Zezula, Juergen; Slany, Astrid; Sieghart, Werner; European Journal of Pharmacology; vol. 301; nb. 1-3; (1996); p. 207 - 214, View in Reaxys; Banerjee, Pradeep K.; Olsen, Richard W.; Snead III, O. Carter; Journal of Pharmacology and Experimental Therapeutics; vol. 291; nb. 1; (1999); p. 361 - 366, View in Reaxys; Huh, Kyung-Hye; Endo, Shuichi; Olsen, Richard W.; European Journal of Pharmacology; vol. 310; nb. 2-3; (1996); p. 225 - 233, View in Reaxys; Zhang, Zhang-Jin; Trivedi, Bakula L.; De Paulis, Tomas; Schmidt, Dennis E.; Hewlett, William A.; Naunyn-Schmiedeberg's Archives of Pharmacology; vol. 365; nb. 2; (2002); p. 123 - 132, View in Reaxys
FTZ
Moaddel; Cloix; Ertem; Wainer; Pharmaceutical Research; vol. 19; nb. 1; (2002); p. 104 - 107, View in Reaxys
FNM
Borron; Monier; Risede; Baud; Human and Experimental Toxicology; vol. 21; nb. 11; (2002); p. 599 - 605, View in Reaxys
Tab. IV
Moore; Pierfitte; Pehourcq; Lagnaoui; Begaud; Clinical Pharmacology and Therapeutics; vol. 69; nb. 6; (2001); p. 445 - 450, View in Reaxys
2
Sigel, Erwin; Schaerer, Martin T.; Buhr, Andreas; Baur, Roland; Molecular Pharmacology; vol. 54; nb. 6; (1998); p. 1097 - 1105, View in Reaxys
Merck 4172
Johnston, Jonathan D.; Price, Sally A.; Bristow, David R.; British Journal of Pharmacology; vol. 124; nb. 7; (1998); p. 1338 - 1340, View in Reaxys
FNZ
Bennett; Simmonds; British Journal of Pharmacology; vol. 117; nb. 1; (1996); p. 87 - 92, View in Reaxys; Uchida, Sunao; Okudaira, Nobuyuki; Nishihara, Kyoko; Iguchi, Yoshinobu; Tan, Xin; Life Sciences; vol. 59; nb. 9; (1996); p. PL117-PL120, View in Reaxys
M 4181
Slany; Zezula; Fuchs; Sieghart; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 2; (1995); p. 99 - 105, View in Reaxys; Hosie, Alastair M.; Sattelle, David B.; British Journal of Pharmacology; vol. 117; nb. 6; (1996); p. 1229 - 1237, View in Reaxys
flunitrazepam
Suzuki, Toshihito; Ito, Takehiko; Wellman, Susan E.; Ho, Ing Kang; European Journal of Pharmacology; vol. 295; nb. 2-3; (1996); p. 169 - 179, View in Reaxys
Fln
Masonis, A. E. Tory; McCarthy, Michael P.; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys
Fig. 1, comp. B1
Maguire; Davies; Loew; European Journal of Pharmacology; vol. 280; nb. 2; (1995); p. 167 - 173, View in Reaxys
FLU
Feigenspan, Andreas; Bormann, Joachim; European Journal of Pharmacology, Molecular Pharmacology Section; vol. 288; nb. 1; (1995); p. 97 - 104, View in Reaxys
FNZ, Negwer 4957
Suzuki, Toshihito; Ito, Takehiko; Wellman, Susan E.; Ho, Ing Kang; Life Sciences; vol. 57; nb. 5; (1995); p. P63 P70, View in Reaxys
Merck 12,4181
Hadingham; Wafford; Thompson; Palmer; Whiting; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 3; (1995); p. 301 - 309, View in Reaxys
(3H)flunitrazepam
Yu; Ticku; Journal of Pharmacology and Experimental Therapeutics; vol. 275; nb. 3; (1995); p. 1442 - 1446, View in Reaxys
Patent-Specific Data (1) Prophetic ComReferences pound prophetic product
Patent; Sumitomo Chemical Company, Limited; US3991048; (1976); (A) English, View in Reaxys; Patent; Schollmayer, Erwin; Sachse, Richard; Braun, Marina; US2008/274061; (2008); (A1) English, View in Reaxys
Druglikeness (1)
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1 of 1
LogP
2.349
H Bond Donors
0
H Bond Acceptors
3
Rotatable Bonds
2
TPSA
78.49
Lipinski Number
4
Veber Number
2
Highest Clinical Phase (1) Highest Clinical Phase Marketed Melting Point (3) 1 of 3
Melting Point [°C]
168 - 170
Patent; Hoffmann-LaRoche; AT334376; (1977); DE2233482; Chem.Abstr.; vol. 78; nb. 111385, View in Reaxys 2 of 3
Melting Point [°C]
170 - 171.5
de Silva et al.; Analytical Chemistry; vol. 48; (1976); p. 10,11, View in Reaxys 3 of 3
Melting Point [°C]
170 - 172
Solvent (Melting Point)
CH2Cl2; hexane
Sternbach,L.H. et al.; Journal of Medicinal Chemistry; vol. 6; (1963); p. 261 - 265, View in Reaxys Adsorption (MCS) (1) 1 of 1
Description (Adsorption (MCS))
Adsorption
Solvent (Adsorption (MCS))
aq. phosphate buffer
Temperature (Adsorption (MCS)) [°C]
20
Comment (Adsorption (MCS))
pH dependence
Partner (Adsorption (MCS))
poly(acrylic acid)-poly(vinylidene fluoride) membrane
Akerman, Satu; Akerman, Kari; Karppi, Jouni; Koivu, Paeivi; Sundell, Annika; Paronen, Petteri; Jaervinen, Kristiina; European Journal of Pharmaceutical Sciences; vol. 9; nb. 2; (1999); p. 137 - 143, View in Reaxys Association (MCS) (2) 1 of 2
Description (Association (MCS))
Stability constant of the complex with ...
Solvent (Association (MCS))
various solvent(s)
Partner (Association (MCS))
immobilized phospholipid membrane
Hollosy, Ferenc; Valko, Klara; Hersey, Anne; Nunhuck, Shenaz; Keri, Gyoergy; Bevan, Chris; Journal of Medicinal Chemistry; vol. 49; nb. 24; (2006); p. 6958 - 6971, View in Reaxys 2 of 2
Description (Association (MCS))
Stability constant of the complex with ...
Solvent (Association (MCS))
various solvent(s)
Partner (Association (MCS))
human serum albumin
Hollosy, Ferenc; Valko, Klara; Hersey, Anne; Nunhuck, Shenaz; Keri, Gyoergy; Bevan, Chris; Journal of Medicinal Chemistry; vol. 49; nb. 24; (2006); p. 6958 - 6971, View in Reaxys Conformation (1)
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Object of Investiga- References tion Conformer equilibrium
Sarrazin et al.; Organic Magnetic Resonance; vol. 7; (1975); p. 89,92, View in Reaxys
Dissociation Exponent (1) 1 of 1
Comment (Dissociation Exponent)
(k')pK
Su; Carstensen; Journal of Pharmaceutical Sciences; vol. 61; (1972); p. 420,421, View in Reaxys Electrical Moment (1) 1 of 1
Description (Electrical Moment)
Dipole moment
Su; Carstensen; Journal of Pharmaceutical Sciences; vol. 61; (1972); p. 420,421, View in Reaxys Liquid/Liquid Systems (MCS) (4) 1 of 4
Description (Liquid/Liquid Distribution between solvent 1 + 2 Systems (MCS)) Temperature (Liquid/ Liquid Systems (MCS)) [°C]
37
Partner (Liquid/Liquid Systems (MCS))
human milk lipid-ultrafiltrate
Atkinson; Begg; Journal of Pharmaceutical Sciences; vol. 77; nb. 9; (1988); p. 796 - 798, View in Reaxys 2 of 4
Description (Liquid/Liquid Distribution between solvent 1 + 2 Systems (MCS)) Temperature (Liquid/ Liquid Systems (MCS)) [°C]
37
Partner (Liquid/Liquid Systems (MCS))
octanol; H2O
Atkinson; Begg; Journal of Pharmaceutical Sciences; vol. 77; nb. 9; (1988); p. 796 - 798, View in Reaxys 3 of 4
Description (Liquid/Liquid Distribution between solvent 1 + 2 Systems (MCS)) Partner (Liquid/Liquid Systems (MCS))
octanol/phosphate buffer (pH = 7.5)
Lacko; Wittke; Arzneimittel-Forschung/Drug Research; vol. 34; nb. 4; (1984); p. 403 - 407, View in Reaxys 4 of 4
Description (Liquid/Liquid Distribution between solvent 1 + 2 Systems (MCS)) Temperature (Liquid/ Liquid Systems (MCS)) [°C]
25
Partner (Liquid/Liquid Systems (MCS))
n-octanol, aq. phosphate buffer
Seiler; Zimmermann; Arzneimittel-Forschung/Drug Research; vol. 33; nb. 11; (1983); p. 1519 - 1522, View in Reaxys Partition octan-1-ol/water (MCS) (5) 1 of 5
log POW
2.56
Location
supporting information
Rankovic, Zoran; Journal of Medicinal Chemistry; vol. 60; nb. 14; (2017); p. 5943 - 5954, View in Reaxys 2 of 5
log POW
2.56
pH
7.4
Location
supporting information
Comment (Partition octan-1-ol/water (MCS))
aq.buffer
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Rankovic, Zoran; Journal of Medicinal Chemistry; vol. 60; nb. 14; (2017); p. 5943 - 5954, View in Reaxys 3 of 5
Partition Constant POW
134
Gatta, Elena; Cupello, Aroldo; Di Braccio, Mario; Grossi, Giancarlo; Robello, Mauro; Scicchitano, Francesca; Russo, Emilio; De Sarro, Giovambattista; Journal of Molecular Neuroscience; vol. 60; nb. 4; (2016); p. 539 - 547, View in Reaxys 4 of 5
log POW
-1.28
Location
supporting information
Sobaska, Anna W.; Zydek, Grazyna; Wlodno, Piotr; Brzeziska, Elzbieta; European Journal of Medicinal Chemistry; vol. 89; (2014); p. 147 - 155, View in Reaxys 5 of 5
log POW
2.14
Hollosy, Ferenc; Valko, Klara; Hersey, Anne; Nunhuck, Shenaz; Keri, Gyoergy; Bevan, Chris; Journal of Medicinal Chemistry; vol. 49; nb. 24; (2006); p. 6958 - 6971, View in Reaxys Solubility (MCS) (1) 1 of 1
Solubility [g·l-1]
0.0169176
Saturation
in solution
Temperature (Solubility (MCS)) [°C]
25
Solvent (Solubility (MCS))
phosphate buffer
Alelyunas, Yun W.; Empfield, James R.; McCarthy, Dennis; Spreen, Russell C.; Bui, Khanh; Pelosi-Kilby, Luciana; Shen, Cindy; Bioorganic and Medicinal Chemistry Letters; vol. 20; nb. 24; (2010); p. 7312 - 7316, View in Reaxys Solution Behaviour (MCS) (1) 1 of 1
Description (Solution Behaviour (MCS))
Solubilizing
Solvent (Solution Behaviour (MCS))
H2O
Temperature (Solution Be- 37 haviour (MCS)) [°C] Comment (Solution Behaviour (MCS))
concentration dependence. Object(s) of Study: time dependence. Object(s) of Study: diagram
Partner (Solution Behaviour (MCS))
oxazepam
Liu, Julie; Journal of Pharmaceutical Sciences; vol. 87; nb. 12; (1998); p. 1632 - 1638, View in Reaxys NMR Spectroscopy (12) 1 of 12
Description (NMR Spectroscopy)
Spectrum
Nucleus (NMR Spectroscopy)
1H
Solvents (NMR Spectroscopy)
CD2Cl2; various solvent(s)
Temperature (NMR Spectroscopy) [°C]
-20
Finner; Zeugner; Milkowski; Archiv der Pharmazie; vol. 317; nb. 12; (1984); p. 1050 - 1053, View in Reaxys 2 of 12
Description (NMR Spectroscopy)
Chemical shifts
Nucleus (NMR Spectroscopy)
13C
Solvents (NMR Spectroscopy)
dimethylsulfoxide-d6
Haran; Tuchagues; Journal of Heterocyclic Chemistry; vol. 17; nb. 7; (1980); p. 1483 - 1488, View in Reaxys; Finner; Zeugner; Milkowski; Archiv der Pharmazie; vol. 317; nb. 12; (1984); p. 1050 - 1053, View in Reaxys
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3 of 12
Description (NMR Spectroscopy)
Chemical shifts
Nucleus (NMR Spectroscopy)
13C
Solvents (NMR Spectroscopy)
CDCl3
Finner; Zeugner; Milkowski; Archiv der Pharmazie; vol. 317; nb. 12; (1984); p. 1050 - 1053, View in Reaxys 4 of 12
Description (NMR Spectroscopy)
Spin-spin coupling constants
Solvents (NMR Spectroscopy)
CD2Cl2
Temperature (NMR Spectroscopy) [°C]
-20
Comment (NMR Spectroscopy)
19F-1H. Solvent(s): further solvent(s)
Finner; Zeugner; Milkowski; Archiv der Pharmazie; vol. 317; nb. 12; (1984); p. 1050 - 1053, View in Reaxys 5 of 12
Description (NMR Spectroscopy)
Spin-spin coupling constants
Solvents (NMR Spectroscopy)
CDCl3
Comment (NMR Spectroscopy)
19F-13C.
Finner; Zeugner; Milkowski; Archiv der Pharmazie; vol. 317; nb. 12; (1984); p. 1050 - 1053, View in Reaxys 6 of 12
Description (NMR Spectroscopy)
Spin-spin coupling constants
Solvents (NMR Spectroscopy)
dimethylsulfoxide-d6
Comment (NMR Spectroscopy)
19F-13C.
Finner; Zeugner; Milkowski; Archiv der Pharmazie; vol. 317; nb. 12; (1984); p. 1050 - 1053, View in Reaxys 7 of 12
Description (NMR Spectroscopy)
Chemical shifts
Nucleus (NMR Spectroscopy)
1H
Solvents (NMR Spectroscopy)
dimethylsulfoxide-d6
Haran; Tuchagues; Journal of Heterocyclic Chemistry; vol. 17; nb. 7; (1980); p. 1483 - 1488, View in Reaxys 8 of 12
Description (NMR Spectroscopy)
Chemical shifts
Nucleus (NMR Spectroscopy)
13C
Solvents (NMR Spectroscopy)
dimethylsulfoxide-d6
Temperature (NMR Spectroscopy) [°C]
28
Sarrazin, Marcel; Faure, Robert; Aubert, Claude; Vincent, Emile-Jean; Journal de Chimie Physique et de Physico-Chimie Biologique; vol. 77; nb. 1; (1980); p. 91 - 94, View in Reaxys 9 of 12
Description (NMR Spectroscopy)
Spin-spin coupling constants
Solvents (NMR Spectroscopy)
CDCl3
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Comment (NMR Spectroscopy)
1H-1H, 19F-1H.
Haran; Tuchagues; Journal of Heterocyclic Chemistry; vol. 17; nb. 7; (1980); p. 1483 - 1488, View in Reaxys 10 of 12
Description (NMR Spectroscopy)
Spin-spin coupling constants
Solvents (NMR Spectroscopy)
CDCl3
Comment (NMR Spectroscopy)
1H-13C, 19F-13C.
Haran; Tuchagues; Journal of Heterocyclic Chemistry; vol. 17; nb. 7; (1980); p. 1483 - 1488, View in Reaxys 11 of 12
Description (NMR Spectroscopy)
Spin-spin coupling constants
Solvents (NMR Spectroscopy)
dimethylsulfoxide-d6
Temperature (NMR Spectroscopy) [°C]
28
Comment (NMR Spectroscopy)
19F-13C.
Sarrazin, Marcel; Faure, Robert; Aubert, Claude; Vincent, Emile-Jean; Journal de Chimie Physique et de Physico-Chimie Biologique; vol. 77; nb. 1; (1980); p. 91 - 94, View in Reaxys 12 of 12
Description (NMR Spectroscopy)
NMR
Ficarra et al.; Bollettino Chimico Farmaceutico; vol. 116; (1977); p. 720,721, 724, 727-729, View in Reaxys; Sarrazin et al.; Organic Magnetic Resonance; vol. 7; (1975); p. 89,92, View in Reaxys IR Spectroscopy (2) 1 of 2
Description (IR Spectroscopy)
Spectrum
Solvent (IR Spectroscopy)
KBr
Comment (IR Spectroscopy)
4000 - 800 cm**(-1)
Huvenne, J. P.; Lacroix, B.; Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy; vol. 44; nb. 1; (1988); p. 109 - 114, View in Reaxys 2 of 2
Description (IR Spectroscopy)
IR
Ficarra et al.; Bollettino Chimico Farmaceutico; vol. 116; (1977); p. 720,721, 724, 727-729, View in Reaxys Mass Spectrometry (5) Description (Mass References Spectrometry) electrospray ionisa- Treu, Axel; Rittner, Miriam; Kemken, Dorit; Schiebel, Hans-Martin; Spiteller, Peter; Dülcks, Thomas; Journal of tion (ESI); liquid Mass Spectrometry; vol. 50; nb. 8; (2015); p. 978 - 986, View in Reaxys chromatography mass spectrometry (LCMS); IT (ion trap); tandem mass spectrometry; spectrum electron impact (EI); fragmentation pattern; spectrum
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys
DESI (desorption Liu, Pengyuan; Lanekoff, Ingela T.; Laskin, Julia; Dewald, Howard D.; Chen, Hao; Analytical Chemistry; vol. 84; electrospray ioniza- nb. 13; (2012); p. 5737 - 5743, View in Reaxys tion); Spectrum LCMS (Liquid chromatography mass spectrome-
Remane, Daniela; Wissenbach, Dirk K.; Meyer, Markus R.; Maurer, Hans H.; Rapid Communications in Mass Spectrometry; vol. 24; nb. 7; (2010); p. 859 - 867, View in Reaxys
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try); Tandem mass spectrometry; Spectrum spectrum; chemical ionization (CI)
Kratzsch, Carsten; Tenberken, Oliver; Peters, Frank T.; Weber, Armin A.; Kraemer, Thomas; Maurer, Hans H.; Journal of Mass Spectrometry; vol. 39; nb. 8; (2004); p. 856 - 872, View in Reaxys
UV/VIS Spectroscopy (10) 1 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
nitric acid
Absorption Maxima (UV/ VIS) [nm]
247; 305
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys 2 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
ethanol
Absorption Maxima (UV/ VIS) [nm]
253; 304
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys 3 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
ethanol
Absorption Maxima (UV/ VIS) [nm]
253; 309
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys 4 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
hydrogenchloride
Absorption Maxima (UV/ VIS) [nm]
280; 360
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys 5 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
perchloric acid
Absorption Maxima (UV/ VIS) [nm]
280; 360
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys 6 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
sulfuric acid
Absorption Maxima (UV/ VIS) [nm]
280; 360
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Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys 7 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
acetic acid
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys 8 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
various solvent(s)
Comment (UV/VIS Spectroscopy)
200 - 400 nm; Remark: T = 25 deg C
Moro; Novillo-Fertrell; Velazquez; Rodriguez; Journal of Pharmaceutical Sciences; vol. 80; nb. 5; (1991); p. 459 - 468, View in Reaxys 9 of 10
Description (UV/VIS Spectroscopy)
Spectrum
Solvent (UV/VIS Spectroscopy)
aq. HCl
Comment (UV/VIS Spectroscopy)
200 - 400 nm; Remark: T = 25 deg C
Moro; Novillo-Fertrell; Velazquez; Rodriguez; Journal of Pharmaceutical Sciences; vol. 80; nb. 5; (1991); p. 459 - 468, View in Reaxys 10 of 10
Description (UV/VIS Spectroscopy)
UV/VIS
Ficarra et al.; Bollettino Chimico Farmaceutico; vol. 116; (1977); p. 720,721, 724, 727-729, View in Reaxys; Levillain et al.; European Journal of Medicinal Chemistry; vol. 10; (1975); p. 433,436, View in Reaxys Fluorescence Spectroscopy (1) Description (Fluo- References rescence Spectroscopy) Spectrum; Maxima
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys
Ecotoxicology (1) 1 of 1
Effect (Ecotoxicology)
chloride channel modulator
Species or Test-System (Ecotoxicology)
Xenopus laevis oocytes
Concentration (Ecotoxicology)
0.1 - 100 μmol/l
Method (Ecotoxicology)
cells injected with cRNA of wild-type Drosophila melanogaster GABA-receptor RDL subunit; perfused with saline; membrane currents (I) induced by 10μM GABA; title comp. added to perfusate
Further Details (Ecotoxicology)
I recorded with two-electrode voltage-clamp (-60 mV; 20 deg C); title comp. effect on GABA-induced I studied
Comment (Ecotoxicology) No effect Hosie, Alastair M.; Sattelle, David B.; British Journal of Pharmacology; vol. 117; nb. 6; (1996); p. 1229 - 1237, View in Reaxys Use (23) Use Pattern Benzodiazepine of a pharmaceutical composition for treating alcoholism
Location
References Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
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Benzodiazepine of a pharmaceutical composition for treating substance addiction
Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
Benzodiazepine of a pharmaceutical composition for treating cocaine abuse
Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
Benzodiazepine of a pharmaceutical composition for treating symptom of alcohol withdrawal
Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
Benzodiazepine of a pharmaceutical composition to cause alcohol aversion
Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
Benzodiazepine of a pharmaceutical composition for treating drug addiction
Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
Benzodiazepine of a pharmaceutical composition for treating alcohol use disorders
Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
Benzodiazepine of a pharmaceutical composition to improve the patient's rehabilitation of chronic alcoholism
Patent; THE UNIVERSITY OF CHICAGO; WO2008/45641; (2008); (A2) English, View in Reaxys
Anxiolytics
Patent; ASTRAZENECA AB; WO2007/86799; (2007); (A1) English, View in Reaxys
muscle spasm
Patent; Lundeen, James E.; US2006/148841; (2006); (A1) English, View in Reaxys
skeletal muscle relaxant
Patent; Lundeen, James E.; US2006/148841; (2006); (A1) English, View in Reaxys
insomnia
Patent; JAGOTEC AG; WO2006/45618; (2006); (A1) English, View in Reaxys
AKR1C2 inhibitor; AKR1C3 inhibitor
Page/Page column 80; 111
Patent; Ghosal, Anima; Kishnani, Narendra S.; Alton, Kevin B.; White, Ronald E.; US2006/276404; (2006); (A1) English, View in Reaxys
Hepatitis C virus (HCV)
Page/Page column 80; 111
Patent; Ghosal, Anima; Kishnani, Narendra S.; Alton, Kevin B.; White, Ronald E.; US2006/276404; (2006); (A1) English, View in Reaxys
Disorder associated Page/Page column with HCV 80; 111
Patent; Ghosal, Anima; Kishnani, Narendra S.; Alton, Kevin B.; White, Ronald E.; US2006/276404; (2006); (A1) English, View in Reaxys
sleep disturbance
Patent; Vela Pharmaceuticals Inc.; EP1202722; (2005); (B1) English, View in Reaxys
pharmaceutical composition with improved safety
Patent; Lotus Pharmaceutical Co., Ltd.; US2005/220715; (2005); (A1) English, View in Reaxys
anxiolytic-hypnotic therapeutic agent
Patent; Lotus Pharmaceutical Co., Ltd.; US2005/220715; (2005); (A1) English, View in Reaxys
Suitable antipsychotic agent
Patent; Yamanouchi Pharma Technologies, Inc.; US2005/208133; (2005); (A1) English, View in Reaxys
hypnotic agent(fastacting benzodiaze-
Patent; SANTARUS, INC.; WO2005/44199; (2005); (A2) English, View in Reaxys
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pine) of pharmaceutical compositions comprising a proton pump inhibitor, a buffering agent, and a sleep aid for the subject is awakened by distress associated with the gastric acid related disorder sleep onset insomnia
Patent; SANTARUS, INC.; WO2005/44199; (2005); (A2) English, View in Reaxys
sleep maintenance insomnia
Patent; SANTARUS, INC.; WO2005/44199; (2005); (A2) English, View in Reaxys
sleep offset insomnia
Patent; SANTARUS, INC.; WO2005/44199; (2005); (A2) English, View in Reaxys
Quantum Chemical Calculations (2) Calculated Proper- Method (Quantum ties Chemical Calculations) Atom distances, an- further quantum gles; Dipole mochemical calcns. ment, dipole moment derivative; Polarizability, polarizability derivatives; Electronic energy levels UV/VIS wave lengths
Location
References
supporting informa- Sobaska, Anna W.; Zydek, Grazyna; Wlodno, Piotr; Brzeziska, Elzbietion ta; European Journal of Medicinal Chemistry; vol. 89; (2014); p. 147 - 155, View in Reaxys
Ab initio calcns. (LCAO, GO SCF, DIM, SAMO, X-à, Hartree-Fock)
Leesakul, Nararak; Pongampai, Sirintip; Kanatharana, Proespichaya; Sudkeaw, Pravit; Tantirungrotechai, Yuthana; Buranachai, Chittanon; Luminescence; vol. 28; nb. 1; (2013); p. 76 - 83, View in Reaxys
Medchem (976) 1 of 976
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
forebrain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flu
Measurement Parameter
Kd
Unit
nM
Quantitative value
5.8
Measurement pX
8.24
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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2 of 976
3 of 976
4 of 976
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
forebrain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flu
Measurement Parameter
Bmax
Unit
pmol/g tissue
Quantitative value
103
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
forebrain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flu
Measurement Parameter
Kd
Unit
nM
Quantitative value
2.3
Measurement pX
8.64
Concomitants: Compound RN
754371; 906818
Concomitants: Compound name
Diazepam; GABA
Concomitants: Compound role
NSB; COM
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
forebrain
Cell Fraction
Membrane
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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5 of 976
6 of 976
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flu
Measurement Parameter
Bmax
Unit
pmol/g tissue
Quantitative value
104
Concomitants: Compound RN
754371; 906818
Concomitants: Compound name
Diazepam; GABA
Concomitants: Compound role
NSB; COM
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
forebrain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flu
Measurement Parameter
IC50
Unit
µM
Quantitative value
5
Measurement pX
5.3
Concomitants: Compound RN
8141905; 702691
Concomitants: Compound name
[35S]-TBPS; [3H]-Flunitrazepam
Concomitants: Compound role
RAD; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
forebrain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flu
Measurement Parameter
IC50
Unit
µM
Quantitative value
2.5
Measurement pX
5.6
Concomitants: Compound RN
702691; 906818
Concomitants: Compound name
[3H]-Flunitrazepam; GABA
Concomitants: Compound role
RAD; COM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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7 of 976
8 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 nM
Measurement Parameter
% Inhibition
Unit
%
Measurement Object
ALL
Quantitative value
18
Measurement pX
1
Concomitants: Compound RN
24140787; 6182863; 3885115
Concomitants: Compound name
Glucose-6-phosphate; Buprenorphine; NADP
Concomitants: Compound role
COM; SUB; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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9 of 976
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 nM
Measurement Parameter
% Inhibition
Unit
%
Measurement Object
ALL
Quantitative value
14
Measurement pX
1
Concomitants: Compound RN
19279336; 24140787; 6182863; 3885115
Concomitants: Compound name
NADPH regenerating System; Glucose-6-phosphate; Buprenorphine; NADP
Concomitants: Compound role
VPR; COM; SUB; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 nM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
14
Measurement pX
1
Metabolite RN
9596605
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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11 of 976
Metabolite name
Norbuprenorphine
Concomitants: Compound RN
24140787; 6182863; 3885115
Concomitants: Compound name
Glucose-6-phosphate; Buprenorphine; NADP
Concomitants: Compound role
COM; SUB; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 nM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
9
Measurement pX
1
Metabolite RN
9596605
Metabolite name
Norbuprenorphine
Concomitants: Compound RN
19279336; 24140787; 6182863; 3885115
Concomitants: Compound name
NADPH regenerating System; Glucose-6-phosphate; Buprenorphine; NADP
Concomitants: Compound role
VPR; COM; SUB; COE
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
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12 of 976
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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14 of 976
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
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15 of 976
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
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Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
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Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Thyrotropin-releasing hormone receptor [human]
Target Synonyms
thyroliberin receptor; thyrotropin-releasing hormone receptor; trh-r; trhr
Target Uniprot ID
p34981
Target, Subunit, Species
Thyrotropin-releasing hormone receptor [human]
Target Mutant/Chimera Details
Thyrotropin-releasing hormone receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
CHO-DXB11 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
-0.5
Measurement pX
1
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Concomitants: Compound RN
24142632; 24142632
Concomitants: Compound name
Thyrotropin releasing hormone; [3H]-TRH; BSA
Concomitants: Compound role
NSB; RAD; COM
Target Name
Thyrotropin-releasing hormone receptor [human]
Target Synonyms
thyroliberin receptor; thyrotropin-releasing hormone receptor; trh-r; trhr
Target Uniprot ID
p34981
Target, Subunit, Species
Thyrotropin-releasing hormone receptor [human]
Target Mutant/Chimera Details
Thyrotropin-releasing hormone receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
CHO-DXB11 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
-13.7
Measurement pX
1
Concomitants: Compound RN
24142632; 24142632
Concomitants: Compound name
Thyrotropin releasing hormone; [3H]-TRH; BSA
Concomitants: Compound role
NSB; RAD; COM
Target Name
Thyrotropin-releasing hormone receptor [human]
Target Synonyms
thyroliberin receptor; thyrotropin-releasing hormone receptor; trh-r; trhr
Target Uniprot ID
p34981
Target, Subunit, Species
Thyrotropin-releasing hormone receptor [human]
Target Mutant/Chimera Details
Thyrotropin-releasing hormone receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
CHO-DXB11 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Name
Flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
-4
Measurement pX
1
Concomitants: Compound RN
24142632; 24142632
Concomitants: Compound name
Thyrotropin releasing hormone; [3H]-TRH; BSA
Concomitants: Compound role
NSB; RAD; COM
Target Name
GABA-A receptor [taurine cattle]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
brain cortex
Cell Fraction
Membrane: homogenate
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.17
Deviation
0.200000
Measurement pX
8.29
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : in vitro; saturation and competitive binding assays performed with <3H>Ro15-1788 or alpidem, 0 deg C; incubation time 90 min; liquid scintillation method
Biological Species/NCBI ID
Rattus norvegicus
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Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-11 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
0.28 - 65.6
Measurement pX
9.55
Maguire; Mechanick; Davies; Ellis; Meredith; Loew; Journal of Pharmacology and Experimental Therapeutics; vol. 273; nb. 2; (1995); p. 842 - 849, View in Reaxys 31 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : chloride channel modulatorBioassay : I recorded with two-electrode voltage-clamp (-60 mV; 20 deg C); title comp. effect on GABA-induced I studied cells injected with cRNA of wildtype Drosophila melanogaster GABA-receptor RDL subunit; perfused with saline; membrane currents (I) induced by 10μM GABA; title comp. added to perfusate
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 µM
Measurement Parameter
Qualitative
Qualitative value
NA
Measurement pX
1
Hosie, Alastair M.; Sattelle, David B.; British Journal of Pharmacology; vol. 117; nb. 6; (1996); p. 1229 - 1237, View in Reaxys 32 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : ion transportTarget : brain from Sprague-Dawley ratBioassay : (36)Cl(-) uptake determined by radioactivity measurement; title comp. effect on M-induced (36)Cl(-) uptake in Co, Ce for NO and SE rats studied adult male rats (12 h light/dark cycle; normal <NO> or in pilocarpin-induced status epilepticus <SE>) sacrificed; cortical (Co) and cerebellar (Ce) synaptoneurosomes prep.; incub. with title comp.+(36)Cl(-)+muscimol (M) in assay buffer (34 deg C; 5 min)
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
ca. 10 percent increase of stimulated (36)Cl(-) influx; similar for Co, Ce in NO, SE
Measurement Parameter
Qualitative
Banerjee, Pradeep K.; Olsen, Richard W.; Snead III, O. Carter; Journal of Pharmacology and Experimental Therapeutics; vol. 291; nb. 1; (1999); p. 361 - 366, View in Reaxys 33 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
central nervous system depressant, action verified by recording the bioelectrical activity of the precentral cortex (in rabbits)
Measurement Parameter
Qualitative
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Stumpf Ch.; Viernstein; Journal of Pharmaceutical Sciences; vol. 79; nb. 12; (1990); p. 1062 - 1064, View in Reaxys 34 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Sprague-Dawley rat cortical synaptoneurosomesBioassay : radioactivity quantitation using liquid scintillation spectrometry rats (4-5 wk); purified cerebrocortical membranes were incubated with 5 nmol/l <35S>-TBPS in the presence of title comp. at 22 deg C for 90 min
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.0500000 mM
Qualitative Results
title comp. exhibited biphasic effect on <35S>-TBPS binding to cortical synaptoneurosomes: at conc. < 1 μmol/l - increased and at conc. > 1 μmol/l - inhibited (diagram)
Measurement Parameter
Qualitative
Masonis; McCarthy; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys 35 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Sprague-Dawley rat cortical synaptoneurosomesBioassay : radioactivity quantitation using liquid scintillation spectrometry rats (4-5 wk); purified cerebrocortical membranes were incubated with 5 nmol/l <35S>-TBPS in the presence of title comp. and 0.025-5 μmol/l GABA at 22 deg C for 90 min
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
50 mM
Measurement Parameter
IC50
Unit
µM
Quantitative value
0.95
Measurement pX
6.02
Masonis; McCarthy; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys 36 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Sprague-Dawley rat cortical synaptoneurosomesBioassay : radioactivity quantitation using liquid scintillation spectrometry rats (4-5 wk); purified cerebrocortical membranes were incubated with 5 nmol/l <35S>-TBPS in the presence of title comp. and 1 μmol/l GABA at 22 deg C for 90 min
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.0500000 mM
Measurement Parameter
IC50
Unit
µM
Quantitative value
0.238
Measurement pX
6.62
Masonis; McCarthy; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys 37 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
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Bioassay Details
Effect : |receptor; binding activity Target : Sprague-Dawley rat cortical synaptoneurosomesBioassay : radioactivity quantitation using liquid scintillation spectrometry rats 4-5 wk; cerebral cortices were homogenized in HEPES/Tris buffer, pH 7.0; incubated with title comp. at 30 deg C in influx buffer containing 0.3 μCi <36Cl-> with increasing concentrations of GABA
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 µM
Measurement Parameter
EC50
Unit
µM
Quantitative value
13.7
Measurement pX
4.86
Masonis; McCarthy; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys 38 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Sprague-Dawley rat cortical synaptoneurosomesBioassay : radioactivity quantitation using liquid scintillation spectrometry rats 4-5 wk; cerebral cortices were homogenized in HEPES/Tris-buffer, pH 7.0, pre-incubated with title comp. (4 deg C 10 min, 30 deg C 5 min), then incubated in the presence of GABA (30 deg C 5 min) in influx buffer containing 0.3 μCi <36Cl->
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 µM
Measurement Parameter
EC50
Unit
µM
Quantitative value
20.6
Measurement pX
4.69
Masonis; McCarthy; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys 39 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor blocking agent Target : Sprague-Dawley rat cortical synaptoneurosomesBioassay : radioactivity quantitation using liquid scintillation spectrometry rats 4-5 wk; cortices were homogenized in HEPES/Tris buffer, pH 7.0, pre-incubated with title comp. and 50 μmol/l stanozolol (4 deg C 10 min),incubated with GABA at 30 deg C 5 min in influx buffer containing 0.3 μCi <36Cl>
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 µM
Measurement Parameter
EC50
Unit
µM
Quantitative value
18.2
Measurement pX
4.74
Masonis; McCarthy; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys 40 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor blocking agent Target : Sprague-Dawley rat cortical synaptoneurosomesBioassay : radioactivity quantitation using liquid scintillation spectrometry rats 4-5 wk; cortices were homo-
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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genized in HEPES/Tris buffer, pH 7.0, pre-incubated with 5 μmol/l stanozolol (4 deg C 10 min), then incubated with title comp. and increasing conc. of GABA at 30 deg C 5 min in influx buffer containing 0.3 μCi <36Cl-> Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 µM
Measurement Parameter
EC50
Unit
µM
Quantitative value
20.2
Measurement pX
4.69
Masonis; McCarthy; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 1; (1996); p. 186 - 193, View in Reaxys 41 of 976
42 of 976
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
IC50
Unit
µM
Quantitative value
635
Measurement pX
3.2
Metabolite RN
8439890
Metabolite name
5-Hydroxyomeprazole
Concomitants: Compound RN
19279336; 3628192
Concomitants: Compound name
NADPH regenerating System; Omeprazole
Concomitants: Compound role
COM; SUB
Substance Action on Target
Radioligand (/ligand)
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43 of 976
44 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
neocortex
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Bmax
Unit
fmol/mg protein
Quantitative value
4239
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
neocortex
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
22
Measurement pX
7.66
Target Name
Cytochrome P450 2E1 [human]
Target Synonyms
4-nitrophenol 2-hydroxylase; cyp2e; cyp2e1; cypiie1; cytochrome p450 2e1; cytochrome p450-j
Target Uniprot ID
p05181
Target PDB ID
3e4e; 3e6i; 3gph; 3koh; 3lc4; 3t3z
Target, Subunit, Species
Cytochrome P450 2E1 [human]
Target Mutant/Chimera Details
Cytochrome P450 2E1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
1000 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
10
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement pX
1
Metabolite RN
1867508
Metabolite name
4-nitrocatechol
Concomitants: Compound RN
24140787; 1281877; 3885115; 84272
Concomitants: Compound name
Glucose-6-phosphate; p-Nitrophenol; NADP; L-(+)-Ascorbic acid
Concomitants: Compound role
COM; SUB; COE; COM
Target Name
Cytochrome P450 2E1 [human]
Target Synonyms
4-nitrophenol 2-hydroxylase; cyp2e; cyp2e1; cypiie1; cytochrome p450 2e1; cytochrome p450-j
Target Uniprot ID
p05181
Target PDB ID
3e4e; 3e6i; 3gph; 3koh; 3lc4; 3t3z
Target, Subunit, Species
Cytochrome P450 2E1 [human]
Target Mutant/Chimera Details
Cytochrome P450 2E1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
250 µM
Measurement Parameter
% Inhibition
Unit
%
Qualitative value
<
Quantitative value
10
Measurement pX
1
Metabolite RN
1867508
Metabolite name
4-nitrocatechol
Concomitants: Compound RN
24140787; 1281877; 3885115; 84272
Concomitants: Compound name
Glucose-6-phosphate; p-Nitrophenol; NADP; L-(+)-Ascorbic acid
Concomitants: Compound role
COM; SUB; COE; COM
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
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47 of 976
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Measurement Object
HIGH
Quantitative value
8.559
Metabolite RN
688458
Metabolite name
Desmethylflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Measurement Object
LOW
Quantitative value
1.341
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Metabolite RN
713494
Metabolite name
3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Measurement Object
HIGH
Quantitative value
1.494
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole mono-
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oxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
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Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Measurement Object
HIGH
Quantitative value
1.762
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
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Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Measurement Object
LOW
Quantitative value
1.036
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement Parameter
Clint
Unit
µL/min/mg protein
Measurement Object
LOW
Quantitative value
1.586
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Measurement Object
HIGH
Quantitative value
0.095
Metabolite RN
688458
Metabolite name
Desmethylflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
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Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Measurement Object
HIGH
Quantitative value
11.1
Measurement pX
4.95
Metabolite RN
688458
Metabolite name
Desmethylflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
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56 of 976
Unit
nmol/min/mg protein
Measurement Object
LOW
Quantitative value
1.27
Metabolite RN
688458
Metabolite name
Desmethylflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Measurement Object
LOW
Quantitative value
108
Measurement pX
3.97
Metabolite RN
688458
Metabolite name
Desmethylflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
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Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Measurement Object
LOW
Quantitative value
0.861
Metabolite RN
713494
Metabolite name
3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement Object
LOW
Quantitative value
642
Measurement pX
3.19
Metabolite RN
713494
Metabolite name
3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Measurement Object
HIGH
Quantitative value
0.42
Metabolite RN
713494
Metabolite name
3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
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Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Measurement Object
HIGH
Quantitative value
34
Measurement pX
4.47
Metabolite RN
713494
Metabolite name
3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
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Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Measurement Object
HIGH
Quantitative value
0.13
Deviation
0.0200000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Measurement Object
HIGH
Quantitative value
0.074
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Deviation
0.0340000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Measurement Object
HIGH
Quantitative value
42
Deviation
17
Measurement pX
4.38
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
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Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Measurement Object
HIGH
Quantitative value
87
Deviation
20
Measurement pX
4.06
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
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Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Measurement Object
LOW
Quantitative value
0.171
Deviation
0.100000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
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Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Measurement Object
LOW
Quantitative value
0.46
Deviation
0.151000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
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Measurement Object
LOW
Quantitative value
165
Deviation
101
Measurement pX
3.78
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6alpha-hydroxylase
Target Uniprot ID
p33261 + p08684
Target PDB ID
4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Measurement Object
LOW
Quantitative value
290
Deviation
84
Measurement pX
3.54
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
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Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2D6 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp2d6; cyp2dl1; cypiid6; cytochrome p450 2d6; cytochrome p450db1; debrisoquine 4-hydroxylase
Target Uniprot ID
p10635
Target PDB ID
2f9q; 3qm4; 3tbg; 3tda; 4wnt; 4wnu; 4wnv; 4wnw; 4xry; 4xrz; 5tft; 5tfu
Target, Subunit, Species
Cytochrome P450 2D6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2D6 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Rate
Qualitative value
NA
Measurement pX
1
Metabolite RN
688458
Metabolite name
Desmethylflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 2E1 [human]
Target Synonyms
4-nitrophenol 2-hydroxylase; cyp2e; cyp2e1; cypiie1; cytochrome p450 2e1; cytochrome p450-j
Target Uniprot ID
p05181
Target PDB ID
3e4e; 3e6i; 3gph; 3koh; 3lc4; 3t3z
Target, Subunit, Species
Cytochrome P450 2E1 [human]
Target Mutant/Chimera Details
Cytochrome P450 2E1 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
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Measurement Parameter
Rate
Qualitative value
NA
Measurement pX
1
Metabolite RN
713494
Metabolite name
3-Hydroxyflunitrazepam
Concomitants: Compound RN
77911
Concomitants: Compound name
NADPH
Concomitants: Compound role
COE
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
nH
Quantitative value
2
Deviation
0.400000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 506008
Concomitants: Compound name
NADPH regenerating System; DMSO
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Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
nH
Quantitative value
2.7
Deviation
0.500000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 506008
Concomitants: Compound name
NADPH regenerating System; DMSO
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
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Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
nH
Quantitative value
1.8
Deviation
0.700000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 741857
Concomitants: Compound name
NADPH regenerating System; Acetonitrile
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
nH
Quantitative value
2.6
Deviation
1.10000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 741857
Concomitants: Compound name
NADPH regenerating System; Acetonitrile
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement Parameter
S50
Unit
µM
Quantitative value
249
Deviation
69.5000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 506008
Concomitants: Compound name
NADPH regenerating System; DMSO
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
286
Deviation
95
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 506008
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Concomitants: Compound name
NADPH regenerating System; DMSO
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
Vmax
Unit
nmol/h/mg protein
Quantitative value
5.9
Deviation
2.60000
Statistical sgnificance (pvalue)
0.008
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 506008
Concomitants: Compound name
NADPH regenerating System; DMSO
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxy-
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genase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
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Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
Vmax
Unit
nmol/h/mg protein
Quantitative value
42.4
Deviation
24.5000
Statistical sgnificance (pvalue)
0.008
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 506008
Concomitants: Compound name
NADPH regenerating System; DMSO
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
313
Deviation
242
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 741857
Concomitants: Compound name
NADPH regenerating System; Acetonitrile
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
201
Deviation
27.1000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 741857
Concomitants: Compound name
NADPH regenerating System; Acetonitrile
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
Vmax
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Unit
nmol/h/mg protein
Quantitative value
21.8
Deviation
20.1000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 741857
Concomitants: Compound name
NADPH regenerating System; Acetonitrile
Concomitants: Compound role
COE; SLV
Target Name
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Synonyms
cholesterol 25-hydroxylase; cyp1a2; cypia2; cytochrome p(3)450; cytochrome p450 1a2; cytochrome p450 4; cytochrome p450-p3 + (r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase + 1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p05177 + p33261 + p08684
Target PDB ID
2hi4 + 4gqs + 1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 1A2 [human]; Cytochrome P450 2C19 [human]; Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 1A2 [human]:Wild; Cytochrome P450 2C19 [human]:Wild; Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
25 µM
Measurement Parameter
Vmax
Unit
nmol/h/mg protein
Quantitative value
101
Deviation
71.4000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3'-Hydroxyflunitrazepam
Concomitants: Compound RN
19279336; 741857
Concomitants: Compound name
NADPH regenerating System; Acetonitrile
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Concomitants: Compound role
COE; SLV
Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
%
Unit
%
Measurement Object
incidence (shakes) (Wet Dog Shakes)
Quantitative value
25
Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
%
Unit
%
Measurement Object
incidence (shakes) (Wet Dog Shakes)
Quantitative value
88
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Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
20 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
%
Unit
%
Measurement Object
incidence (shakes) (Wet Dog Shakes)
Quantitative value
100
Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
60 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
%
Unit
%
Measurement Object
incidence (shakes) (Wet Dog Shakes)
Quantitative value
100
Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
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Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
shakes per 30 min. (Wet Dog Shakes)
Quantitative value
6.4
Deviation
4.10000
Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
shakes per 30 min. (Wet Dog Shakes)
Quantitative value
28.7
Deviation
3.20000
Statistical sgnificance (pvalue)
0.05
Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
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Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
20 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
shakes per 30 min. (Wet Dog Shakes)
Quantitative value
48
Deviation
9.50000
Statistical sgnificance (pvalue)
0.05
Target Name
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Synonyms
5-hydroxytryptamine receptor + gaba-a receptor
Target, Subunit, Species
5-hydroxytryptamine receptor [Rattus norvegicus]; GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
5-hydroxytryptamine receptor [Rattus norvegicus]:Wild; GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus; Rattus norvegicus
Target Transfection
Non Transfected; Non Transfected
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Specific behaviour
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
60 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
shakes per 30 min. (Wet Dog Shakes)
Quantitative value
47.7
Deviation
2.20000
Statistical sgnificance (pvalue)
0.05
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Bioassay Category
Metabolism/Transport
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.25000 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
BB ratio
Measurement Object
Concentration
Quantitative value
1.15
Metabolite RN
899031; 899030; 754932
Metabolite name
1'-Hydroxymidazolam; 4-Hydroxymidazolam; N-Desmethylclobazam
Arendt; Greenblatt; Liebisch; Luu; Paul; Psychopharmacology; vol. 93; nb. 1; (1987); p. 72 - 76, View in Reaxys 91 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1.66
Measurement pX
8.78
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Arendt; Greenblatt; Liebisch; Luu; Paul; Psychopharmacology; vol. 93; nb. 1; (1987); p. 72 - 76, View in Reaxys 92 of 976
Bioassay Category
Metabolism/Transport
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.25000 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
fu
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Quantitative value
0.193
Metabolite RN
899031; 899030; 754932
Metabolite name
1'-Hydroxymidazolam; 4-Hydroxymidazolam; N-Desmethylclobazam
Arendt; Greenblatt; Liebisch; Luu; Paul; Psychopharmacology; vol. 93; nb. 1; (1987); p. 72 - 76, View in Reaxys 93 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dosing Regimen
Single
Measurement Parameter
Fa(%)
Unit
%
Quantitative value
90
Bergstroem, Christel A. S.; Strafford, Melissa; Lazorova, Lucia; Avdeef, Alex; Luthman, Kristina; Artursson, Per; Journal of Medicinal Chemistry; vol. 46; nb. 4; (2003); p. 558 - 570, View in Reaxys 94 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
reduction of the increase of HVA induced by chlorpromazine (male mice, 1 mg/kg, i.p.)
Measurement Parameter
Qualitative
Pieri; Schaffner; Scherschlicht; Polc; Sepinwall; Davidson; Moehler; Cumin; Da Prada; Burkard; Keller; Mueller; Gerold; Cook; Haefely; Arzneimittel-Forschung/Drug Research; vol. 31; nb. 12 a; (1981); p. 2180 - 2201, View in Reaxys 95 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |pharmacokinetics Target : human liver microsomesBioassay : CLint: intrinsic clearance CLint determined for both oxidative pathways and summed to give CLint
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
CLint: 5.9 μL/min/mg protein
Measurement Parameter
Qualitative
Hakooz, Nancy; Ito, Kiyomi; Rawden, Helen; Gill, Helen; Lemmers, Lynn; Boobis, Alan R.; Edwards, Robert J.; Carlile, David J.; Lake, Brian G.; Houston, J. Brian; Pharmaceutical Research; vol. 23; nb. 3; (2006); p. 533 - 539, View in Reaxys 96 of 976
Substance Effect
Analgesic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Target : Crl:CD-1(ICR)BR miceBioassay : percent = 100 * number of nonresponders / number in group in vivo; Crl:CD-1(ICR)BR mice (18-25 g) grouphoused (5-15/cage); title comp. was given 30 min before i.p. injection of endothelin-1 (0.1 mg/kg); single abdominal constriction response recorded
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Route of Adm.
oral administration
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Qualitative Results
less than 50 percent antagonism at the maximum dose (10 mg/kg) applied
Measurement Parameter
Qualitative
Raffa, Robert B.; Schupsky, James J.; Lee, David K. H.; Jacoby, Henry I.; Journal of Pharmacology and Experimental Therapeutics; vol. 278; nb. 1; (1996); p. 1 - 7, View in Reaxys 97 of 976
Target Name
endothelin receptor [human]
Target Synonyms
endothelin receptor
Target, Subunit, Species
endothelin receptor [human]
Target Mutant/Chimera Details
endothelin receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor blocking agent Bioassay : SPA=scintillation proximity assay in vitro; SPA kit with endothelin preparation from human placental membrane was used; binding of 125I-endothelin (2,000 Ci/mmol) measured
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
100 µM
Measurement Parameter
Qualitative
Qualitative value
NA
Measurement pX
1
Raffa, Robert B.; Schupsky, James J.; Lee, David K. H.; Jacoby, Henry I.; Journal of Pharmacology and Experimental Therapeutics; vol. 278; nb. 1; (1996); p. 1 - 7, View in Reaxys 98 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Target : Wistar rat cortex homogenateBioassay : nonspecific binding was determined in the presence of unlabeled flumazenil; Ki: affinity of the displacer GABAA receptor binding determined by displacement of <3H>flumazenil; brain cortex homogenate incubated with title comp. and <3H>flumazenil for 35 min at 37 deg C with/without 50 μmol/l GABA; sample filtered and radioactivity on filter determined
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
µg/L
Quantitative value
9.8
Measurement pX
7.5
Visser; Wolters; Gubbens-Stibbe; Tukker; Van Der Graaf; Peletier; Danhof, Meindert; Journal of Pharmacology and Experimental Therapeutics; vol. 304; nb. 1; (2003); p. 88 - 101, View in Reaxys 99 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |pharmacokinetics Bioassay : control: vehicle; PK/PD: pharmacokinetic - pharmacodynamic model; PK/PD model converged yielding estimates of both in vivo receptor affinity (KPD) and in vivo intrinsic efficacy (ePD) conc.-effect relationships; arterial blood samples taken in time points after title comp. injection; plasma conc. of title comp. determined by HPLC with UV detection; pharmacodynamics estimated by recording EEG (electroencephalogram) parameters
Biological Species/NCBI ID
Rattus norvegicus
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Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
2.20000 mg/kg
Substance Route of Adm.
intravenous administration
Qualitative Results
title comp. PK/PD modeling revealed monophasic concentration-EEG effect relationships with potency (EC50) 35.5 ng/ml and intrinsic activity 9.3 μV; KPD = 9.5 ng/ml and ePD = 0.50
Measurement Parameter
Qualitative
Visser; Wolters; Gubbens-Stibbe; Tukker; Van Der Graaf; Peletier; Danhof, Meindert; Journal of Pharmacology and Experimental Therapeutics; vol. 304; nb. 1; (2003); p. 88 - 101, View in Reaxys 100 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |protein binding Bioassay : fu: free fraction, was calculated by dividing of free title comp. conc. in ultrafiltrate by total (bound and free) conc. in plasma blood was spiked with title comp. and placed in water bath at 37 deg C for 30 min; then blood samples were hemolyzed and centrifuged using ultrafiltration device; conc. of title comp. in the blood, plasma and ultrafiltrate was measured by HPLC
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
fu
Quantitative value
26.6
Visser; Wolters; Gubbens-Stibbe; Tukker; Van Der Graaf; Peletier; Danhof, Meindert; Journal of Pharmacology and Experimental Therapeutics; vol. 304; nb. 1; (2003); p. 88 - 101, View in Reaxys 101 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Epsilon) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (epsilon) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Epsilon) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Epsilon) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
% Inhibition
Unit
%
Measurement Object
of GABA EC20
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Quantitative value
0
Measurement pX
1
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p14867
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
# 220 nM
Measurement Parameter
% Stimulation
Unit
%
Measurement Object
of GABA EC20
Quantitative value
121
Deviation
9.30000
Measurement pX
7.94
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p14867
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Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.2
Measurement pX
8.66
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
mg/kg
Quantitative value
2.1
Deviation
0.400000
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
OCV; OCV
Benavides; Peny; Durand; Arbilla; Scatton; Journal of Pharmacology and Experimental Therapeutics; vol. 263; nb. 2; (1992); p. 884 - 896, View in Reaxys 105 of 976
Bioassay Category
In Vivo (Animal models)
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Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
mg/kg
Quantitative value
2.5
Deviation
0.400000
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
OCV; OCV
Benavides; Peny; Durand; Arbilla; Scatton; Journal of Pharmacology and Experimental Therapeutics; vol. 263; nb. 2; (1992); p. 884 - 896, View in Reaxys 106 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
mg/kg
Quantitative value
2.6
Deviation
0.600000
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
OCV; OCV
Benavides; Peny; Durand; Arbilla; Scatton; Journal of Pharmacology and Experimental Therapeutics; vol. 263; nb. 2; (1992); p. 884 - 896, View in Reaxys 107 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
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Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
mg/kg
Quantitative value
2.8
Deviation
0.600000
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
OCV; OCV
Benavides; Peny; Durand; Arbilla; Scatton; Journal of Pharmacology and Experimental Therapeutics; vol. 263; nb. 2; (1992); p. 884 - 896, View in Reaxys 108 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Sprague Dawley rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
mg/kg
Quantitative value
3.5
Deviation
0.800000
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
OCV; OCV
Benavides; Peny; Durand; Arbilla; Scatton; Journal of Pharmacology and Experimental Therapeutics; vol. 263; nb. 2; (1992); p. 884 - 896, View in Reaxys 109 of 976
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
cerebellum
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Cells/Cell Lines
nerve cell
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Measurement Object
HIGH
Quantitative value
3.9
Measurement pX
8.41
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Benavides; Peny; Durand; Arbilla; Scatton; Journal of Pharmacology and Experimental Therapeutics; vol. 263; nb. 2; (1992); p. 884 - 896, View in Reaxys 110 of 976
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
cerebellum
Cells/Cell Lines
nerve cell
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Measurement Object
LOW
Quantitative value
2.4
Measurement pX
8.62
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Benavides; Peny; Durand; Arbilla; Scatton; Journal of Pharmacology and Experimental Therapeutics; vol. 263; nb. 2; (1992); p. 884 - 896, View in Reaxys
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Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18507 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
7.96
Deviation
0.150000
Measurement pX
8.1
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
L-M(TK-) cell line
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Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
22.44
Deviation
2.47000
Measurement pX
7.65
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2 + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18507 + p47869 + p47870
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.34
Deviation
0.160000
Measurement pX
8.48
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
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Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
L-M(TK-) cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
9.57
Deviation
1.59000
Measurement pX
8.02
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p31644 + p18507 + p47870
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
7.02
Deviation
0.290000
Measurement pX
8.15
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p28472 + p31644 + p18507
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
L-M(TK-) cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
12.08
Deviation
1.33000
Measurement pX
7.92
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
29
Deviation
11.2000
Measurement pX
7.54
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
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Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
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Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
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Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p08220 + p10064
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
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Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
24
Deviation
10.4000
Measurement pX
7.62
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p08220 + p10064
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]
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Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p08220 + p10064
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p08220 + p10064
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
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Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p08220 + p10064
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p08220 + p10064
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
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Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
3.3
Deviation
0.700000
Measurement pX
8.48
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Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
123
Deviation
3.20000
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 1) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 1) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 1) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 1) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
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Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
26.5
Deviation
20.3000
Measurement pX
7.58
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 1) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 1) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 1) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 1) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
81
Deviation
9.60000
Concomitants: Compound RN
906818
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Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
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Target, Subunit, Species
GABA-A receptor (Gamma 2L) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 1) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 1) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 1) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 1) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 1) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 1) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 1) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 1) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 1) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 1) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 1) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 1) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 1) [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 1) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 1) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 1) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Quantitative value
100
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
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Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Qualitative value
#
Quantitative value
0.79
Measurement pX
9.1
Concomitants: Compound RN
754371; 4763661; 906818
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788; GABA
Concomitants: Compound role
NSB; RAD; AGO
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Qualitative value
#
Quantitative value
1.51
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement pX
8.82
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
114
Measurement pX
6.94
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [mammal];+Gamma-aminobutyric acid receptor subunit gamma-2 [mammal];+Gamma-aminobutyric acid receptor subunit alpha-1 [mammal]
Target Synonyms
gamma-aminobutyric acid receptor subunit beta-3 + gamma-aminobutyric acid receptor subunit gamma-2 + gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [mammal]; Gamma-aminobutyric acid receptor subunit beta-3 [mammal]; Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [mammal]:Wild
Target Species (Bioactivity)
mammal
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
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Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.2
Deviation
0.200000
Measurement pX
8.28
Concomitants: Compound RN
6182319; 4763661
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [mammal];+Gamma-aminobutyric acid receptor subunit gamma-2 [mammal];+Gamma-aminobutyric acid receptor subunit alpha-2 [mammal]
Target Synonyms
gamma-aminobutyric acid receptor subunit beta-3 + gamma-aminobutyric acid receptor subunit gamma-2 + gamma-aminobutyric acid receptor subunit alpha-2
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [mammal]; Gamma-aminobutyric acid receptor subunit beta-3 [mammal]; Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [mammal]:Wild
Target Species (Bioactivity)
mammal
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.5
Deviation
0.200000
Measurement pX
8.6
Concomitants: Compound RN
6182319; 4763661
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [mammal];+Gamma-aminobutyric acid receptor subunit gamma-2 [mammal];+Gamma-aminobutyric acid receptor subunit alpha-3 [mammal]
Target Synonyms
gamma-aminobutyric acid receptor subunit beta-3 + gamma-aminobutyric acid receptor subunit gamma-2 + gamma-aminobutyric acid receptor subunit alpha-3
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Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [mammal]; Gamma-aminobutyric acid receptor subunit beta-3 [mammal]; Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [mammal]:Wild
Target Species (Bioactivity)
mammal
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
4.8
Deviation
1
Measurement pX
8.32
Concomitants: Compound RN
6182319; 4763661
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [mammal];+Gamma-aminobutyric acid receptor subunit alpha-5 [mammal];+Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]
Target Synonyms
gamma-aminobutyric acid receptor subunit beta-3 + gamma-aminobutyric acid receptor subunit alpha-5 + gamma-aminobutyric acid receptor subunit gamma-2
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [mammal]; Gamma-aminobutyric acid receptor subunit beta-3 [mammal]; Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]:Wild
Target Species (Bioactivity)
mammal
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.11
Deviation
0.230000
Measurement pX
8.68
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Concomitants: Compound RN
6182319; 4763661
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [mammal];+Gamma-aminobutyric acid receptor subunit alpha-6 [mammal];+Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]
Target Synonyms
gamma-aminobutyric acid receptor subunit beta-3 + gamma-aminobutyric acid receptor subunit alpha-6 + gamma-aminobutyric acid receptor subunit gamma-2
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6 [mammal]; Gamma-aminobutyric acid receptor subunit beta-3 [mammal]; Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit alpha-6 [mammal]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [mammal]:Wild
Target Species (Bioactivity)
mammal
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
10000
Measurement pX
1
Concomitants: Compound RN
6182319; 6182319
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-4513
Concomitants: Compound role
NSB; RAD
Bioassay Category
Metabolism/Transport
Bioassay Name
Binding
Biological Species/NCBI ID
human
Population State
Healthy
Organs/Tissues
plasma
Substance RN
702691View in Reaxys
Substance Name
C-4200
Substance Dose
5 µg/mL
Measurement Parameter
fu
Quantitative value
0.166
Deviation
0.0240000
Lucek; Coutinho; Molecular Pharmacology; vol. 12; nb. 4; (1976); p. 612 - 619, View in Reaxys
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Target Name
Nuclear receptor subfamily 1 group I member 3 [human]
Target Synonyms
car; constitutive activator of retinoid response; constitutive active response; constitutive androstane receptor; nr1i3; nuclear receptor subfamily 1 group i member 3; orphan nuclear receptor mb67
Target Uniprot ID
q14994
Target PDB ID
1xv9; 1xvp
Target, Subunit, Species
Nuclear receptor subfamily 1 group I member 3 [human]
Target Mutant/Chimera Details
Nuclear receptor subfamily 1 group I member 3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Transactivation assay
Cells/Cell Lines
JHH-7 cell line
Substance RN
702691View in Reaxys
Substance Name
19
Substance Dose
10 µM
Measurement Parameter
Fold-increase
Qualitative value
<
Quantitative value
1.5
Target Name
Cytochrome P450 3A [human]
Target Synonyms
cytochrome p450 3a
Target, Subunit, Species
Cytochrome P450 3A [human]
Target Mutant/Chimera Details
Cytochrome P450 3A [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
% Inhibition
Unit
%
Qualitative value
<
Quantitative value
10
Measurement pX
1
Metabolite RN
899031
Metabolite name
1'-Hydroxymidazolam
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Concomitants: Compound RN
625572; 506008; 506008; 77911; 77911
Concomitants: Compound name
Midazolam; DMSO; DMSO; NADPH; NADPH
Concomitants: Compound role
SUB; SLV; SLV; COE; COE
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Cytosol
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
60 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
51
Measurement pX
4.24
Metabolite RN
18416447
Metabolite name
M28
Concomitants: Compound RN
19279336; 10502086
Concomitants: Compound name
NADPH regenerating System; [14C]-Boceprevir
Concomitants: Compound role
COM; SUB
; (2011); Type: Clinical Pharmacology Biopharmaceutics Review, Lab: NDA202258, Owner: SCHERING, Number: 000, View in Reaxys 153 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Wistar rat brain sectionsBioassay : number of brain areas from mesencephalon, cerebral cortex, hippocampus, and cerebellum; Tris-HCl, pH 7.4; 4 deg C; incubated for 40 min; characterization of BZ2 carried out directly by co-incubation with 1 μmol/l zolpidem (specifically binds to BZ1) in vitro; autoradiographical saturation kinetic study of overall benzodiazepine (BZ) binding sites as well as of benzodiazepine binding site subtype 1 (BZ1) and subtype 2 (BZ2); <3H>labelled title comp.
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.0800000 nM
Qualitative Results
KD, equilibrium association constant KDs as well as receptor densities Bmax (32-328 fmol/mg protein) for several brain areas presented; statistically significant differences in affinities of BZ2 with respect to BZ and BZ1 binding sites were mainly found in cortical 1.1 - 4.8 nmol/l
Measurement Parameter
Qualitative
Soria, Carlos; Revilla, Victoria; Candelas, Maria Adoracion; Calvo, Pedro; Fernandez-Lopez, Arsenio; Biochemical Pharmacology; vol. 50; nb. 10; (1995); p. 1619 - 1625, View in Reaxys 154 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
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Bioassay Details
Effect : |receptor; binding activity Target : Trachemys scripta, freshwater turtleBioassay : turtles (0.75-1.3 kg) subjected to a 100 percent N2 atmosphere for up to 24 h; after exposure, cerebral cortex dissected out, and saturation binding assay for GABA/benzodiazepine receptors performed using <3H>labelled title comp.
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
no signif. change in dissociation constant over 24 h of anoxia; signif. increase in receptor density after 12 h of anoxia
Measurement Parameter
Qualitative
Lutz; Leone-Kabler; American Journal of Physiology - Regulatory Integrative and Comparative Physiology; vol. 268; nb. 5 37-5; (1995); p. R1332-R1335, View in Reaxys 155 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : effect on extracellular acidification rateBioassay : 30 min washout to remove GABA, followed by 30 min exposure to title comp.; 15 min washout performed between each GABA concentration; measurement of CGC extracellular acidification rate in cytosensor microphysiometer primary CGC prepared from 8-day-post-natal rats; cells (9-12E5 cells/transwell cup) at 8 days in vitro assessed for response to increasing concentrations of GABA (0.010-1000 μmol/l, 25 deg C, 33 s stimulation) in presence of title comp.
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
granule cell
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
100 nM
Qualitative Results
title comp. significantly potentiated GABA-stimulated increase in the extracellular acidification rate at low GABA concentration (0.010-1 μmol/l); graphical representation; title comp. had no effect in the absence of GABA
Measurement Parameter
Qualitative
Brown, Maria J.; Wood, Martyn D.; Coldwell, Martyn C.; Bristow, David R.; British Journal of Pharmacology; vol. 121; nb. 1; (1997); p. 71 - 76, View in Reaxys 156 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |drug interaction Bioassay : opioids: morphine sulfate, buprenorphine hydrochloride, methadone hydrochloride; MLD: median lethal dose randomized blind MLD studies; up-and-down method performed in quadruplicate; four series; rats pretreated with title comp., 30 min thereafter treated with opioids; animals examined repeatedly during first 4 h after injection, then daily for mortality
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
40 mg/kg
Substance Route of Adm.
intraperitoneal administration
Qualitative Results
MLDs decreased signif. by pretreatm. with title comp. in case of buprenorphine and methadone; no signif. effects on MLD of morphine; admin. of title comp. signif. prolonged time to death in buprenorphine-treated rats; no signif. effects in other cases
Measurement Parameter
Qualitative
Borron; Monier; Risede; Baud; Human and Experimental Toxicology; vol. 21; nb. 11; (2002); p. 599 - 605, View in Reaxys 157 of 976
Substance Effect
Hypnogenic
Bioassay Category
In Vivo (Animal models)
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Bioassay Name
In vivo Measurement
Bioassay Details
Bioassay : polysomnographic recordings were made at selected nights (1, 2, 3, 4, 7, 10, 12, 14); delta as 0.3-3.0 Hz sigma as 10.0-15.0 Hz and beta as 20.0-28.0 Hz were defined five healthy young volunteers (age 19-22); experiment performed on a fourteen day schedule in a double blind design; from the fourth to the tenth night title comp. administered before bedtime; otherwise placebo was administered at that time
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Route of Adm.
oral administration
Qualitative Results
alteration of delta, sigma and beta EEG induced by title comp. for 7 consecutive nights; sleep EEG subjected to fast Fourier transform analysis; sigma was enhanced without regard to delta amount, but beta only during epochs contng. low delta
Measurement Parameter
Qualitative
Uchida, Sunao; Okudaira, Nobuyuki; Nishihara, Kyoko; Iguchi, Yoshinobu; Tan, Xin; Life Sciences; vol. 59; nb. 9; (1996); p. PL117-PL120, View in Reaxys 158 of 976
159 of 976
Target Name
enzyme
Target Synonyms
enzyme
Target, Subunit, Species
enzyme
Target Mutant/Chimera Details
enzyme:Wild
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |enzyme; inhib. of Bioassay : S-tetralol: S-(+)-1,2,3,4-tetrahydronaphth-1-ol enzyme incubated with title comp. in 0.1 mol/l potassium phosphate buffer (pH 7.4) containing 0.25 mmol/l NADP(1+) and 1.0 mmol/l S-tetralol; dehydrogenase activity determined
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
IC50
Unit
µM
Quantitative value
32
Measurement pX
4.49
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : binding affinity; inhibition ofTarget : rat brain cortex membranesBioassay : ref.: rolipram (IC50: 0.058 μmol/l) membranes treated with <3H>-rolipram; title comp. added; displacement of <3H>-rolipram by title comp. determined by binding assay
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 µM
Qualitative Results
percent inhibition of binding affinity by title comp. was 14.7 percent; table
Measurement Parameter
Qualitative
Collado, M. Carmen; Beleta, Jorge; Martinez, Ester; Miralpeix, Montse; Domenech, Teresa; Palacios, Jose Maria; Hernandez, Jesus; British Journal of Pharmacology; vol. 123; nb. 6; (1998); p. 1047 - 1054, View in Reaxys 160 of 976
Target Name
cGMP-specific 3',5'-cGMP phosphodiesterase 3
Target Synonyms
cgmp-specific 3',5'-cgmp phosphodiesterase 3; ddpde3; pde3; phosphodiesterase 3
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Target, Subunit, Species
cGMP-specific 3',5'-cGMP phosphodiesterase 3
Target Mutant/Chimera Details
cGMP-specific 3',5'-cGMP phosphodiesterase 3:Wild
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |enzyme; inhib. of Bioassay : control: vehicle; ref.: rolipram (IC50: 242 μmol/l) enzyme treated with title comp.; cyclic nucleotide phosphodiesterase activities measured by phosphodiesterase assay
Biological Species/NCBI ID
guinea pig
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
200 µM
Qualitative Results
percent inhibition of enzyme by title comp. was 35 percent; table
Measurement Parameter
Qualitative
Collado, M. Carmen; Beleta, Jorge; Martinez, Ester; Miralpeix, Montse; Domenech, Teresa; Palacios, Jose Maria; Hernandez, Jesus; British Journal of Pharmacology; vol. 123; nb. 6; (1998); p. 1047 - 1054, View in Reaxys 161 of 976
Target Name
cAMP-specific 3',5'-cAMP phosphodiesterase 4
Target Synonyms
camp-specific 3',5'-camp phosphodiesterase 4; ddpde4; pde4; phosphodiesterase 4
Target, Subunit, Species
cAMP-specific 3',5'-cAMP phosphodiesterase 4
Target Mutant/Chimera Details
cAMP-specific 3',5'-cAMP phosphodiesterase 4:Wild
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |enzyme; inhib. of Bioassay : control: vehicle; ref.: rolipram (IC50: 0.32 μmol/l) enzyme treated with title comp.; cyclic nucleotide phosphodiesterase activities measured by phosphodiesterase assay
Biological Species/NCBI ID
guinea pig
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
200 µM
Measurement Parameter
IC50
Unit
µM
Quantitative value
64
Measurement pX
4.19
Collado, M. Carmen; Beleta, Jorge; Martinez, Ester; Miralpeix, Montse; Domenech, Teresa; Palacios, Jose Maria; Hernandez, Jesus; British Journal of Pharmacology; vol. 123; nb. 6; (1998); p. 1047 - 1054, View in Reaxys 162 of 976
Target Name
cGMP-specific 3',5'-cyclic phosphodiesterase
Target Synonyms
cgb-pde; cgmp-binding cgmp-specific phosphodiesterase; cgmp-specific 3',5'-cyclic phosphodiesterase; dmpde5/6; dmpde6; pde5; pde5a; pde6
Target, Subunit, Species
cGMP-specific 3',5'-cyclic phosphodiesterase
Target Mutant/Chimera Details
cGMP-specific 3',5'-cyclic phosphodiesterase:Wild
Target Transfection
Non Transfected
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Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |enzyme; inhib. of Bioassay : control: vehicle; ref.: rolipram (IC50: 125 μmol/l) enzyme treated with title comp.; cyclic nucleotide phosphodiesterase activities measured by phosphodiesterase assay
Biological Species/NCBI ID
dog
Cells/Cell Lines
thrombocyte
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
200 µM
Measurement Parameter
IC50
Unit
µM
Quantitative value
66
Measurement pX
4.18
Collado, M. Carmen; Beleta, Jorge; Martinez, Ester; Miralpeix, Montse; Domenech, Teresa; Palacios, Jose Maria; Hernandez, Jesus; British Journal of Pharmacology; vol. 123; nb. 6; (1998); p. 1047 - 1054, View in Reaxys 163 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : respiration; inhibition ofBioassay : BUP: buprenorphine catheters placed to anesthetized rats 22 h before test; title comp. admin. alone or in combination with 30 mg/kg of BUP; arterial blood samples collected before and at 1.5-120 min after title comp. admin.; blood gases measured
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
30 mg/kg
Substance Route of Adm.
intravenous administration
Qualitative Results
title comp. with BUP caused respiratory depression; no effect on respiration of title comp. alone; decrease in arterial PaO2, blood pH and increase in PaCO2 after dose of title comp. and BUP; conc. of PaO2, PaCO2, HCO3 and pH values vs time; figures
Measurement Parameter
Qualitative
Megarbane, Bruno; Pirnay, Stephane; Borron, Stephen W.; Trout, Herve; Monier, Claire; Risede, Patricia; Boschi, Gabrielle; Baud, Frederic J.; Toxicology Letters; vol. 157; nb. 3; (2005); p. 211 - 219, View in Reaxys 164 of 976
Target Name
Cytochrome P450 [human]
Target Synonyms
cyp1a2; cyp1b1; cyp2a6v2; cyp2b6; cyp2c19; cyp2j2; cyp3a4; cyp3a43; cyp3a43/cyp3a4; cyp4f2; cytochrome p450
Target Uniprot ID
a0n0x8; a4f4k4; c1kde6; c1kde7; c1kde8; d0ezn6; d0ezn7; d0ezn8; e7emt5; o95743; q0vhd5; q13120; q53g23; q5j9b1; q6nwu3; q767a3; q7z2g5; q7z446; q7z447; q7z448; q86sk2; q86sk3; q96rx4; q9unx8
Target, Subunit, Species
Cytochrome P450 [human]
Target Mutant/Chimera Details
Cytochrome P450 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
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Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Cytosol
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
Unit
%
Qualitative value
<
Quantitative value
20
Measurement pX
1
Metabolite RN
3596645
Metabolite name
6-beta-naltrexol
Concomitants: Compound RN
3580333; 3580333; 77911
Concomitants: Compound name
Naltrexone hydrochloride; Naltrexone hydrochloride; NADPH
Concomitants: Compound role
SUB; SUB; COE
Target Name
Cytochrome P450 2A6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase; cyp2a3; cyp2a6; cypiia6; cytochrome p450 2a6; cytochrome p450 iia3; cytochrome p450(i)
Target Uniprot ID
p11509
Target PDB ID
1z10; 1z11; 2fdu; 2fdv; 2fdw; 2fdy; 3ebs; 3t3q; 3t3r; 4ejj; 4rui
Target, Subunit, Species
Cytochrome P450 2A6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2A6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
1920.5
Measurement pX
2.72
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
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166 of 976
167 of 976
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2A6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase; cyp2a3; cyp2a6; cypiia6; cytochrome p450 2a6; cytochrome p450 iia3; cytochrome p450(i)
Target Uniprot ID
p11509
Target PDB ID
1z10; 1z11; 2fdu; 2fdv; 2fdw; 2fdy; 3ebs; 3t3q; 3t3r; 4ejj; 4rui
Target, Subunit, Species
Cytochrome P450 2A6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2A6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
297.5
Measurement pX
3.53
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2A6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase; cyp2a3; cyp2a6; cypiia6; cytochrome p450 2a6; cytochrome p450 iia3; cytochrome p450(i)
Target Uniprot ID
p11509
Target PDB ID
1z10; 1z11; 2fdu; 2fdv; 2fdw; 2fdy; 3ebs; 3t3q; 3t3r; 4ejj; 4rui
Target, Subunit, Species
Cytochrome P450 2A6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2A6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
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169 of 976
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.13
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2A6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase; cyp2a3; cyp2a6; cypiia6; cytochrome p450 2a6; cytochrome p450 iia3; cytochrome p450(i)
Target Uniprot ID
p11509
Target PDB ID
1z10; 1z11; 2fdu; 2fdv; 2fdw; 2fdy; 3ebs; 3t3q; 3t3r; 4ejj; 4rui
Target, Subunit, Species
Cytochrome P450 2A6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2A6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
1
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2A6 [human]
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Target Synonyms
1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase; cyp2a3; cyp2a6; cypiia6; cytochrome p450 2a6; cytochrome p450 iia3; cytochrome p450(i)
Target Uniprot ID
p11509
Target PDB ID
1z10; 1z11; 2fdu; 2fdv; 2fdw; 2fdy; 3ebs; 3t3q; 3t3r; 4ejj; 4rui
Target, Subunit, Species
Cytochrome P450 2A6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2A6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
nL/min/pmol target
Quantitative value
0.44
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2A6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; coumarin 7-hydroxylase; cyp2a3; cyp2a6; cypiia6; cytochrome p450 2a6; cytochrome p450 iia3; cytochrome p450(i)
Target Uniprot ID
p11509
Target PDB ID
1z10; 1z11; 2fdu; 2fdv; 2fdw; 2fdy; 3ebs; 3t3q; 3t3r; 4ejj; 4rui
Target, Subunit, Species
Cytochrome P450 2A6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2A6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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172 of 976
Measurement Parameter
Clint
Unit
nL/min/pmol target
Quantitative value
0.52
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
154.6
Measurement pX
3.81
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450
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3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
173 of 976
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
285.7
Measurement pX
3.54
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
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Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
1.54
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
15.7
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
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Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
nL/min/pmol target
Quantitative value
54.95
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
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Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
nL/min/pmol target
Quantitative value
9.96
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2B6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; cyp2b6; cypiib6; cytochrome p450 2b6; cytochrome p450 iib1
Target Uniprot ID
p20813
Target PDB ID
3ibd; 3qoa; 3qu8; 3ua5; 4i91; 4rql; 4rrt; 4zv8; 5uap; 5uda; 5uec; 5ufg
Target, Subunit, Species
Cytochrome P450 2B6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2B6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
100.7
Measurement pX
4
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2B6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; cyp2b6; cypiib6; cytochrome p450 2b6; cytochrome p450 iib1
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Target Uniprot ID
p20813
Target PDB ID
3ibd; 3qoa; 3qu8; 3ua5; 4i91; 4rql; 4rrt; 4zv8; 5uap; 5uda; 5uec; 5ufg
Target, Subunit, Species
Cytochrome P450 2B6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2B6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.27
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2B6 [human]
Target Synonyms
1,4-cineole 2-exo-monooxygenase; cyp2b6; cypiib6; cytochrome p450 2b6; cytochrome p450 iib1
Target Uniprot ID
p20813
Target PDB ID
3ibd; 3qoa; 3qu8; 3ua5; 4i91; 4rql; 4rrt; 4zv8; 5uap; 5uda; 5uec; 5ufg
Target, Subunit, Species
Cytochrome P450 2B6 [human]
Target Mutant/Chimera Details
Cytochrome P450 2B6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
nL/min/pmol target
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Quantitative value
2.68
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
59.6
Measurement pX
4.22
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
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Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.83
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
nL/min/pmol target
Quantitative value
13.93
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
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Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2C9 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cholesterol 25-hydroxylase; cyp2c10; cyp2c9; cypiic9; cytochrome p-450mp; cytochrome p450 2c9; cytochrome p450 mp-4; cytochrome p450 mp-8; cytochrome p450 pb-1; s-mephenytoin 4-hydroxylase
Target Uniprot ID
p11712
Target PDB ID
1og2; 1og5; 1r9o; 4nz2; 5a5i; 5a5j
Target, Subunit, Species
Cytochrome P450 2C9 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C9 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
50.2
Measurement pX
4.3
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2C9 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cholesterol 25-hydroxylase; cyp2c10; cyp2c9; cypiic9; cytochrome p-450mp; cytochrome p450 2c9; cytochrome p450 mp-4; cytochrome p450 mp-8; cytochrome p450 pb-1; s-mephenytoin 4-hydroxylase
Target Uniprot ID
p11712
Target PDB ID
1og2; 1og5; 1r9o; 4nz2; 5a5i; 5a5j
Target, Subunit, Species
Cytochrome P450 2C9 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C9 [human]:Wild
Target Species (Bioactivity)
human
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Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.38
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2C9 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cholesterol 25-hydroxylase; cyp2c10; cyp2c9; cypiic9; cytochrome p-450mp; cytochrome p450 2c9; cytochrome p450 mp-4; cytochrome p450 mp-8; cytochrome p450 pb-1; s-mephenytoin 4-hydroxylase
Target Uniprot ID
p11712
Target PDB ID
1og2; 1og5; 1r9o; 4nz2; 5a5i; 5a5j
Target, Subunit, Species
Cytochrome P450 2C9 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C9 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
nL/min/pmol target
Quantitative value
7.57
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
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Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
256.8
Deviation
13.9000
Measurement pX
3.59
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
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Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
207.6
Deviation
31
Measurement pX
3.68
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
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Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/mg protein
Quantitative value
3671.9
Deviation
1494
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Vmax
Unit
pmol/min/mg protein
Quantitative value
478.9
Deviation
150.400
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
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Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Quantitative value
2.56
Deviation
0.980000
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
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Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Quantitative value
13.69
Deviation
5.09000
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
nH
Quantitative value
1.4
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Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 2C9 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cholesterol 25-hydroxylase; cyp2c10; cyp2c9; cypiic9; cytochrome p-450mp; cytochrome p450 2c9; cytochrome p450 mp-4; cytochrome p450 mp-8; cytochrome p450 pb-1; s-mephenytoin 4-hydroxylase
Target Uniprot ID
p11712
Target PDB ID
1og2; 1og5; 1r9o; 4nz2; 5a5i; 5a5j
Target, Subunit, Species
Cytochrome P450 2C9 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C9 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
AHH-1 TK+/- cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
nH
Quantitative value
2.7
Metabolite RN
688458
Metabolite name
desmethylflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
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Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
nH
Quantitative value
1.2
Deviation
0.0300000
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0 µM
Measurement Parameter
nH
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Quantitative value
1.7
Deviation
0.120000
Metabolite RN
688458; 713494
Metabolite name
desmethylflunitrazepam; 3-hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
retina
Cell Fraction
Homogenate
Substance RN
702691View in Reaxys
Substance Name
[3H]-flunitrazepam
Measurement Parameter
Bmax
Quantitative value
4
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
retina
Cell Fraction
Nucleus and cell debris (P1 fraction)
Substance RN
702691View in Reaxys
Substance Name
[3H]-flunitrazepam
Measurement Parameter
Bmax
Quantitative value
4
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Substance Action on Target
Radioligand (/ligand)
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Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
retina
Cell Fraction
Synaptosome (P2 fraction)
Substance RN
702691View in Reaxys
Substance Name
[3H]-flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/mg
Quantitative value
0.08
Deviation
0.00200000
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
retina
Cell Fraction
Synaptosome (P2 fraction)
Substance RN
702691View in Reaxys
Substance Name
[3H]-flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/mg
Quantitative value
0.103
Deviation
0.00200000
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Target Name
Histamine H1 receptor [Rattus norvegicus]
Target Synonyms
h1r; hh1r; histamine h1 receptor; hrh1
Target Uniprot ID
p31390
Target, Subunit, Species
Histamine H1 receptor [Rattus norvegicus]
Target Mutant/Chimera Details
Histamine H1 receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
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Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
17000
Measurement pX
4.77
Concomitants: Compound RN
435610; 87215
Concomitants: Compound name
[3H]-Pyrilamine; Triprolidine
Concomitants: Compound role
RAD; NSB
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
forebrain
Cell Fraction
P2 fraction: membrane
Substance RN
702691View in Reaxys
Substance Name
RO 5-4200 (flunitrazepam)
Measurement Parameter
IC50
Unit
nM
Quantitative value
5
Measurement pX
8.3
Concomitants: Compound RN
754371; 754371
Concomitants: Compound name
Diazepam; [3H]-Diazepam
Concomitants: Compound role
NSB; RAD
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
retina
Cell Fraction
Homogenate
Substance RN
702691View in Reaxys
Substance Name
[3H]-flunitrazepam
Measurement Parameter
Bmax
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Unit
pmol/mg
Quantitative value
0.085
Deviation
0.0130000
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
retina
Cell Fraction
Nucleus and cell debris (P1 fraction)
Substance RN
702691View in Reaxys
Substance Name
[3H]-flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/mg
Quantitative value
0.015
Deviation
0.00200000
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18507 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
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Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Deviation
0.900000
Measurement pX
8.41
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1.1
Deviation
0.500000
Measurement pX
8.96
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
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Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.9
Deviation
1.50000
Measurement pX
8.23
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit gamma-2
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
% Stimulation
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Unit
%
Quantitative value
121
Deviation
9
Measurement pX
7.28
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : brain membranes from bovine brainBioassay : membranes prepared from cerebral cortex or cerebellum by known method; radioligand labelling; binding place determined after hydroxylamine cleavage
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
photoaffinity labelling in receptor population occurs within residues 1-103 of the α1 sequence
Measurement Parameter
Qualitative
Duncalfe; Dunn; European Journal of Pharmacology; vol. 298; nb. 3; (1996); p. 313 - 319, View in Reaxys 209 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cerebral cortical neurones from 15-d-old fetus of ddY mouseBioassay : neurones cultured with 1 μM α-CCE at 37 deg C for 12 h or not (control); the extensively washed membrane fractions from neurones then incubated with various concns. of (3H)title comp. at 2 deg C for 60 min; Scatchard analysis neopallium free of meninges dissected from a 15-day-old fetus, minced, trypsinized and centrifuged, cultured at 37 deg C for 3 ds; <95 percent of the cells confirmed to be neurons by immunohistochemical approch
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.0310000 nM
Qualitative Results
high affinity binding site for (3H)-title comp. Bmax 1.51 pmol/mg protein (absence of α-CCE); Bmax 1.06 pmol/mg protein (presence of α-CCE) decrease after 3 h exposure to α-CCE; Kd 12.0 nM (control) 11.2
Measurement Parameter
Qualitative
Katsura, Masashi; Ohkuma, Seitaro; Jun, Xu; Kuriyama, Kinya; European Journal of Pharmacology; vol. 298; nb. 1; (1996); p. 71 - 77, View in Reaxys 210 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cerebral cortical neurones from 15-d-old fetus of ddY mouseBioassay : neurones cultured with 1 μM α-CCE at 37 deg C for 12 h, or not (control); the extensively washed membrane fractions from neurones then incubated with 1 nM (3H)-title comp. at 2 deg C for 60 min in presence of various concns. of non labeled title comp. neopallium free of meninges dissected from a 15-day-old fetus, minced, trypsinized and centrifuged, cultured at 37 deg C for 3 ds; <95 percent of the cells confirmed to be neurons by immunohistochemical approch
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 µM
Measurement Parameter
IC50
Unit
nM
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Quantitative value
2.8
Measurement pX
8.55
Katsura, Masashi; Ohkuma, Seitaro; Jun, Xu; Kuriyama, Kinya; European Journal of Pharmacology; vol. 298; nb. 1; (1996); p. 71 - 77, View in Reaxys 211 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : modulation of GABA currentsTarget : Xenopus laevis oocytes injected with α1α1α2sBioassay : adult Xenopus laevis stage V and VI oocytes nuclei inj. with recombinant human GABAA receptors; title compound effect examined using two-electrode voltage-clamp method; on control GABA EC20 responses with a preapplication time of 30 sec
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
ca. 125 percent modulation of control GABA EC20 (fig. given)
Measurement Parameter
Qualitative
Wafford; Thompson; Thomas; Sikela; Wilcox; Whiting; Molecular Pharmacology; vol. 50; nb. 3; (1996); p. 670 - 678, View in Reaxys 212 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : modulation of GABA currentsTarget : Xenopus laevis oocytes injected with α4α1α2sBioassay : adult Xenopus laevis stage V and VI oocytes nuclei inj. with recombinant human GABAA receptors; title compound effect examined using two-electrode voltage-clamp method; on control GABA EC20 responses with a preapplication time of 30 sec
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Measurement Parameter
Qualitative
Qualitative value
NA
Measurement pX
1
Wafford; Thompson; Thomas; Sikela; Wilcox; Whiting; Molecular Pharmacology; vol. 50; nb. 3; (1996); p. 670 - 678, View in Reaxys 213 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : modulation of GABA currentsTarget : Xenopus laevis oocytes injected with α6α1α2sBioassay : adult Xenopus laevis stage V and VI oocytes nuclei inj. with recombinant human GABAA receptors; title compound effect examined using two-electrode voltage-clamp method; on control GABA EC20 responses with a preapplication time of 30 sec
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Measurement Parameter
Qualitative
Qualitative value
NA
Measurement pX
1
Wafford; Thompson; Thomas; Sikela; Wilcox; Whiting; Molecular Pharmacology; vol. 50; nb. 3; (1996); p. 670 - 678, View in Reaxys 214 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
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Bioassay Details
Effect : |metabolic Target : Harlan Spraque-Dawley ratBioassay : coronal sections of the freezed brain, thickness 12 μ; incubation with 1nM 3H radiolabelled flunitrazepam for various time and temperatures (4 and 22 Celsius degrees); the sections were then exposed for 2 weeks
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
incubation 90 min in -4 Celsius degrees: the best results; in pentobarbital-tolerant rats: the activity observed in most regions of the brain; in pentobarbital-dependent rats: the binding sites increased in relatively large number of brain regions
Measurement Parameter
Qualitative
Suzuki, Toshihito; Ito, Takehiko; Wellman, Susan E.; Ho, Ing Kang; European Journal of Pharmacology; vol. 295; nb. 2-3; (1996); p. 169 - 179, View in Reaxys 215 of 976
216 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam 68
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.2
Measurement pX
8.66
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
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217 of 976
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam 68
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.5
Measurement pX
8.6
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam 68
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
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218 of 976
219 of 976
Unit
nM
Quantitative value
4.5
Measurement pX
8.35
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p28472 + p31644 + p18507
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam 68
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.1
Measurement pX
8.68
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-6 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p28472 + q16445 + p18507
Target PDB ID
4cof
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220 of 976
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-6 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam 68
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
2000
Measurement pX
5.7
Concomitants: Compound RN
6182319
Concomitants: Compound name
[3H]-Ro 15-4513
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cerebellar membranes from Sprague-Dawley ratsBioassay : membrane homogenates of cerebellum from rats prepared according to Endo and Olsen (J.Neurochem. 59,1444 (1993)); supernatant or pellet suspension incubated with ligand; radioligand assay
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
binding activities of immunoprecipitates in Fig.
Measurement Parameter
Qualitative
Huh, Kyung-Hye; Endo, Shuichi; Olsen, Richard W.; European Journal of Pharmacology; vol. 310; nb. 2-3; (1996); p. 225 233, View in Reaxys 221 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : in vitro; equilibrium binding assay; <3H>labeled title comp. (spec. act. >80 Ci/mmol); intact cells; Tris-citrate buffer (pH 7.4); 0 deg C; scintillation counting
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
granule cell
Substance RN
702691View in Reaxys
Substance Name
31870
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Substance Dose
0.300000 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
3.28
Measurement pX
8.48
Vale, Carmen; Pomes, Anna; Rodriguez-Farre, Eduard; Sunol, Cristina; European Journal of Pharmacology; vol. 319; nb. 2-3; (1997); p. 343 - 353, View in Reaxys 222 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Bioassay : nonspecific binding detn. in presence of 10 μmol/l of title comp.; Scatchard analysis; filtered; radioactivity counted by liquid scintillation counter synaptic membrane prepn. from bovine brain in Tris-citrate buffer pH 7.1, contg. 0.2-0.3 mg protein incubated with 0.5 nmol/l (3H)title comp. in presence or absence of N3-phenacyluridine at 4 deg C for 1 h
Biological Species/NCBI ID
taurine cattle
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
IC50
Unit
µM
Quantitative value
129
Measurement pX
3.89
Kimura, Toshiyuki; Kuze, Jiro; Watanabe, Kazuhito; Kondo, Shigemi; Ho, Ing Kang; Yamamoto, Ikuo; European Journal of Pharmacology; vol. 311; nb. 2-3; (1996); p. 265 - 269, View in Reaxys 223 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |protein binding Target : polyclonal antibody to benzodiazepinesBioassay : 3H labeled title comp. phosphate buffer, pH 7.4; room temp.; 30 min incubation; quantified by fluorescence correlation microscopy
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Kd
Unit
nM
Quantitative value
272.7
Measurement pX
6.56
Hegener, Oliver; Jordan, Randolf; Haeberlein, Hanns; Journal of Medicinal Chemistry; vol. 47; nb. 14; (2004); p. 3600 3605, View in Reaxys 224 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |drug interaction Bioassay : rats trained for 3 d; 24 h after rats treated with title comp. for 45 min and with gaboxadol (Ca 1-10 mg/kg, s.c.) for 30 min; rotarod motor coordination test performed
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
5 mg/kg
Substance Route of Adm.
intraperitoneal administration
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Qualitative Results
no synergistic interaction between title comp. and gaboxadol observed
Measurement Parameter
Qualitative
Voss, Jeppe; Sanchez, Connie; Michelsen, Signe; Ebert, Bjarke; European Journal of Pharmacology; vol. 482; nb. 1-3; (2003); p. 215 - 222, View in Reaxys 225 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |drug interaction Bioassay : 24 h before title comp. administration rats trained for 3 d; treatment with ethanol (1.0 g/kg, i.p.) for 15 min and with title comp. for 45 min; rotarod motor coordination test performed
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.156000 mg/kg
Substance Route of Adm.
intraperitoneal administration
Qualitative Results
supra-additive effect of title comp. at dose of 0.63 mg/kg and ethanol observed
Measurement Parameter
Qualitative
Voss, Jeppe; Sanchez, Connie; Michelsen, Signe; Ebert, Bjarke; European Journal of Pharmacology; vol. 482; nb. 1-3; (2003); p. 215 - 222, View in Reaxys 226 of 976
Substance Effect
Behavioural Symptoms
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Bioassay : 24 h before title comp. administration rats trained for 3 d; treatment with title comp. for 45 min; rotarod motor coordination test performed
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.160000 mg/kg
Substance Route of Adm.
intraperitoneal administration
Measurement Parameter
ED50
Unit
mg/kg
Quantitative value
4.63
Voss, Jeppe; Sanchez, Connie; Michelsen, Signe; Ebert, Bjarke; European Journal of Pharmacology; vol. 482; nb. 1-3; (2003); p. 215 - 222, View in Reaxys 227 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |agonist Target : Xenopus laevis oocyte expressing α1α2α2 GABAA receptorBioassay : oocyte preperfused with title comp. for 3 min before addition of GABA, voltage clamped at -60 mV; Cl(-) current measured
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-10 M
Measurement Parameter
EC50
Unit
nM
Quantitative value
5.9
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement pX
8.23
Davies, Martin; Newell, J. Glen; Derry, Jason M. C.; Martin, Ian L.; Dunn, Susan M. J.; Molecular Pharmacology; vol. 58; nb. 4; (2000); p. 756 - 762, View in Reaxys 228 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |agonist Target : Xenopus laevis oocyte expressing α1α2α3 GABAA receptorBioassay : oocyte preperfused with title comp. for 3 min before addition of GABA, voltage clamped at -60 mV; Cl(-) current measured
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-10 M
Measurement Parameter
EC50
Unit
nM
Quantitative value
14.8
Measurement pX
7.83
Davies, Martin; Newell, J. Glen; Derry, Jason M. C.; Martin, Ian L.; Dunn, Susan M. J.; Molecular Pharmacology; vol. 58; nb. 4; (2000); p. 756 - 762, View in Reaxys 229 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |agonist Target : Sprague-Dawley rat cortical wedgeBioassay : further study: influence of ethanol and Ro 15-1788 on title comp. effect; GABA: α-aminobutyric acid wedges were superfused for 20 min with superfusion buffer containing title comp.; GABA/gaboxadol EC20 applied with title comp.; number of spikes during last 12 min counted; relative frequency calculated
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1000 nM
Qualitative Results
% potentiation of GABA/gaboxadol EC20 response by title comp. were 18.6/60, resp.; ethanol not potentiated GABA/gaboxadol EC20 response; Ro 15-1788 partially antagonised title comp. effect for GABA, completely abolished for gaboxadol EC20; fig., table
Measurement Parameter
Qualitative
Storustovu, Signe i; Ebert, Bjarke; European Journal of Pharmacology; vol. 467; nb. 1-3; (2003); p. 49 - 56, View in Reaxys 230 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |agonist Target : Xenopus laevis oocytes expressing human GABAA receptorsBioassay : test system comprising GABAA (α-aminobutyric acid type A) receptors are composed of α1α3α2S subunits; further study: influence of ethanol and Ro 15-1788 on title comp. effect oocytes superfused with solution containing title comp.; after 60 s, gaboxadol EC20 and title comp. were co-applied; potentiation of gaboxadol EC20 responses by title comp. was investigated
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 nM
Qualitative Results
title comp. potentiated gaboxadol EC20 responses by 124%; ethanol was not able to bring further potentiation for title comp.; Ro 15-1788 increased gaboxadol EC20 response by 10%; fig.
Measurement Parameter
Qualitative
Storustovu, Signe i; Ebert, Bjarke; European Journal of Pharmacology; vol. 467; nb. 1-3; (2003); p. 49 - 56, View in Reaxys 231 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450
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3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
232 of 976
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
nmol/min/1e6 cells
Quantitative value
0.019
Deviation
0.0150000
Metabolite RN
688458; 713494; 751823; 759300
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
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Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
nmol/min/1e6 cells
Quantitative value
0.14
Deviation
0.130000
Metabolite RN
688458; 713494; 751823; 759300
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
66
Deviation
45
Metabolite RN
713494; 759300
Metabolite name
3-Hydroxyflunitrazepam; (+/-)-Temazepam
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
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Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
nH
Quantitative value
2.1
Metabolite RN
713494; 759300
Metabolite name
3-Hydroxyflunitrazepam; (+/-)-Temazepam
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
43
Deviation
17
Measurement pX
4.37
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Metabolite RN
688458; 751823
Metabolite name
Desmethylflunitrazepam; N-Desmethyldiazepam
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clint
Unit
µL/min/1e6 cells
Quantitative value
0.442
Metabolite RN
688458; 751823
Metabolite name
Desmethylflunitrazepam; N-Desmethyldiazepam
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
ligand affinities for calmodulin and tropinon C and effect on calmodulin dependent stimulation of cyclic nucleotide phosphodiesterase
Measurement Parameter
Qualitative
Tkachuk; Baldenkov; Feoktistov; Men'shikov; Quast; Herzig; Arzneimittel-Forschung/Drug Research; vol. 37; nb. 9; (1987); p. 1013 - 1017, View in Reaxys 238 of 976
Substance Effect
Hypnogenic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Bioassay : high intra-individual consistency of relative EEG power patterns on 4DN and 7DN was observed; double blind design 5 healthy volunteers (Ss, 19 - 22 yrs); title comp. administered for seven consecutive nights 30 min before bed time; sleep EEG from baseline night, the initial title comp. night the fourth (4DN) and the seventh (7DN) nights analysed
Biological Species/NCBI ID
human
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Substance RN
702691View in Reaxys
Substance Name
31870
Substance Route of Adm.
oral administration
Qualitative Results
during non rapid eye movement (NREM) sleep of 4DN and 7DN sigma band activity was similarly enhanced in every S; in rapid eye movement sleep of 4DN and 7DN the beta band was enhanced with larger variations among Ss (table, diagram)
Measurement Parameter
Qualitative
Uchida, Sunao; Okudaira, Nobuyuki; Nishihara, Kyoko; Iguchi, Yoshinobu; Life Sciences; vol. 58; nb. 10; (1996); p. PL199-PL205, View in Reaxys 239 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : receptor photoaffinity labelingTarget : R-3327 Dunning prostatic tumorBioassay : tumor sublines AT-1, H, G; mitochondrial and microsomal fractions; peripheral benzodiazepine receptor; <N-methyl-3H>title comp.; preincubation (dark, 15 min); 60 min. UV irradiation; 10 μmol/l of unlabeled title comp.; electrophoresis
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
100 nM
Measurement Parameter
Qualitative
Qualitative value
ND
Alenfall, Jan; Batra, Satish; Biochemical Pharmacology; vol. 51; nb. 8; (1996); p. 1009 - 1013, View in Reaxys 240 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Guinea pig cortex membraneBioassay : specific <3H>ligand binding to receptors was determined by subtracting the total from the non-specific counts; non-specific ligand: 10 μM diazepam; receptor: benzodiazepine cortex from male animals 200-400 g weight; equilibrium binding assays; 200-400 μg of protein was used; after 0.5-2 h the assays were terminated by collecting the samples on glass-fiber filters with a cell harvester
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Kd
Unit
nM
Quantitative value
2.1
Measurement pX
8.68
Gordon; Nigam; Weitz; Dave; Doctor; Ved; Journal of applied toxicology : JAT; vol. 21 Suppl 1; (2001); p. S47-51, View in Reaxys 241 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Cmax
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Unit
ng/mL
Quantitative value
6
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 242 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Concentration
Unit
ng/mL
Quantitative value
0.4
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 243 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Tmax
Unit
hour
Quantitative value
1
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 244 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Dosing Regimen
Single
Measurement Parameter
AUC
Unit
ng.h/mL
Quantitative value
57
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 245 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
16
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 246 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Cmax
Unit
ng/mL
Quantitative value
6
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 247 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
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Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Concentration
Unit
ng/mL
Quantitative value
0.4
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 248 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Tmax
Unit
hour
Quantitative value
1
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 249 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
AUC
Unit
ng.h/mL
Quantitative value
57
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 250 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
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Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
16
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 251 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
AUC i/AUC
Quantitative value
1.28
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 252 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
t1/2 el i/t1/2 el
Quantitative value
1.56
Concomitants: Compound RN
75279
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Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 253 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Cmax i/Cmax
Quantitative value
1.17
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 254 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Concentration i/Concentration
Quantitative value
1.75
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys
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Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
AUC i/AUC
Quantitative value
1.25
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 256 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
t1/2 el i/t1/2 el
Quantitative value
2
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 257 of 976
Target Name
Cholecystokinin receptor [Rattus norvegicus]
Target Synonyms
cholecystokinin receptor
Target, Subunit, Species
Cholecystokinin receptor [Rattus norvegicus]
Target Mutant/Chimera Details
Cholecystokinin receptor [Rattus norvegicus]:Wild
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Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
pancreas
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
IC50
Unit
µM
Qualitative value
>
Quantitative value
100
Measurement pX
1
Concomitants: Compound RN
1719757
Concomitants: Compound name
Dithiothreitol
Concomitants: Compound role
COM
Target Name
Cholecystokinin receptor [guinea pig]
Target Synonyms
cholecystokinin receptor
Target, Subunit, Species
Cholecystokinin receptor [guinea pig]
Target Mutant/Chimera Details
Cholecystokinin receptor [guinea pig]:Wild
Target Species (Bioactivity)
guinea pig
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
guinea pig
Organs/Tissues
brain
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
IC50
Unit
µM
Qualitative value
>
Quantitative value
100
Measurement pX
1
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
IC50
Unit
µM
Quantitative value
0.0017
Measurement pX
8.77
Concomitants: Compound RN
754371
Concomitants: Compound name
[3H]-Diazepam
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : GABAA receptors, recombinant, cloned on HEK 293 cellsBioassay : HEK: human embryonic kidney cells transfected with cDNAs for α1-, α3-, α2 subunits of GABAA receptors and their combinations; incubated in Tris-citrate buffer, 90 min, 4 deg C, with <3H>-labeled title comp.; non-specific binding in pres. diazepam; scintillation
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
20 nM
Qualitative Results
high affinity binding only to membranes from cells transfected with α1α2- and α1α3α2-subunits, low affinity binding also to non-transfected cells; diagram
Measurement Parameter
Qualitative
Zezula; Slany; Sieghart; European Journal of Pharmacology; vol. 301; nb. 1-3; (1996); p. 207 - 214, View in Reaxys 261 of 976
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
hippocampus
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
32
Measurement Parameter
IC50
Unit
nM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Quantitative value
2.7
Deviation
0.100000
Measurement pX
8.57
Concomitants: Compound RN
754371; 702691
Concomitants: Compound name
Diazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
cerebellum
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
32
Measurement Parameter
IC50
Unit
nM
Quantitative value
2.9
Deviation
0.700000
Measurement pX
8.54
Concomitants: Compound RN
754371; 702691
Concomitants: Compound name
Diazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus; Rattus norvegicus
Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Synaptosome
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
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Measurement Parameter
Bmax
Unit
µmol/mg protein
Quantitative value
765
Deviation
78
Concomitants: Compound RN
205542
Concomitants: Compound name
Melatonin
Concomitants: Compound role
APR
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus; Rattus norvegicus
Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Synaptosome
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Bmax
Unit
µmol/mg protein
Quantitative value
870
Deviation
22
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
APR
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Cmax
Unit
ng/mL
Quantitative value
7
Concomitants: Compound RN
75279
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Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 266 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Concentration
Unit
ng/mL
Quantitative value
0.7
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 267 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Tmax
Unit
hour
Quantitative value
2
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 268 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
AUC
Unit
ng.h/mL
Quantitative value
73
Statistical sgnificance (pvalue)
0.043
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 269 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Non Smoker
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
25
Statistical sgnificance (pvalue)
0.043
Concomitants: Compound RN
75279
Concomitants: Compound name
Erythromycin
Concomitants: Compound role
ICP
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Luurila, Harri; Olkkola, Klaus T.; Neuvonen, Pertti J.; Pharmacology and Toxicology; vol. 78; nb. 2; (1996); p. 117 - 122, View in Reaxys 270 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : tissues homogenized, centrifuged twice, incubated in 50 mM Tris HCl, PH 7.7 at 0 deg C with 5 nM <3H>Ro 15-4513 + 65 μM alpidem; radiactivity determined by liquid scintillation
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
15
Measurement pX
7.82
Maguire; Davies; Loew; European Journal of Pharmacology; vol. 280; nb. 2; (1995); p. 167 - 173, View in Reaxys 271 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : filtered, filters were subjected to scintillation counting cells were cultured and transfected with cDNA encoding for α1, α3 or α2 subunits of GABAA receptors, membranes extracted, frozen membranes thawed, centrifuged, incubated in buffer with 3H-radiolabeled title comp. at 4 degC for 90 min
Biological Species/NCBI ID
human
Cells/Cell Lines
HEK293-EBNA cell line
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
high affinity binding sites observed in membranes from cells cotransfected with α1, α3, and α2 subunits while no binding sites were formed from cells transfected with these subunits
Measurement Parameter
Qualitative
Slany; Zezula; Tretter; Sieghart; Molecular Pharmacology; vol. 48; nb. 3; (1995); p. 385 - 391, View in Reaxys 272 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Target : Wistar rat cerebral synaptic membranesBioassay : nonspecific binding determined by parallel incubations with 1E-5 M diazepam rat weighing 180-220 g; incubation of membrane preparations with <3H> labelled title comp. together with 1E-9 - 1E-3 M S-adenosyl-L-homocysteine, S-adenosylmethionine and 5'-methylthioadenosine, resp. for 1 h at 4 deg C; liquid scintillation
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
8 nM
Qualitative Results
inhibition of title comp. binding with Ki value of 7.9 μM and 15.8 μM, resp. by S-adenosylhomocysteine and 5'-methylthioadenosine; no effect of S-adenosylmethionine
Measurement Parameter
Qualitative
Tsvetnitsky; Campbell; Gibbons; European Journal of Pharmacology; vol. 282; nb. 1-3; (1995); p. 255 - 258, View in Reaxys 273 of 976
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
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Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
µM
Quantitative value
0.005
Deviation
0.00150000
Measurement pX
8.3
Concomitants: Compound RN
754371; 754371
Concomitants: Compound name
Diazepam; [3H]-Diazepam
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
GABA ratio
Measurement Object
IC50
Quantitative value
1.41
Concomitants: Compound RN
754371; 754371; 906818
Concomitants: Compound name
Diazepam; [3H]-Diazepam; GABA
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Concomitants: Compound role
NSB; RAD; AGO
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
brain cortex
Cells/Cell Lines
nerve cell
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
3.8
Measurement pX
8.42
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
[3H]-Flunitrazepam; Flumazenil
Concomitants: Compound role
RAD; NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Chbb:THOM rat
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
IC50
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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278 of 976
Unit
nM
Quantitative value
3.2
Deviation
1.30000
Measurement pX
8.49
Concomitants: Compound RN
754371; 702691
Concomitants: Compound name
Diazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Chbb:THOM rat
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
5.9
Deviation
0.800000
Measurement pX
8.23
Concomitants: Compound RN
839277
Concomitants: Compound name
[3H]-Brotizolam
Concomitants: Compound role
RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
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Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Chbb:THOM rat
Organs/Tissues
cerebellum
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[H3]-flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
1.4
Deviation
0.130000
Measurement pX
8.85
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Chbb:THOM rat
Organs/Tissues
cerebellum
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[H3]-flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/g
Quantitative value
57
Deviation
5.40000
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
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Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Chbb:THOM rat
Organs/Tissues
hippocampus
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[H3]-flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
1.48
Deviation
0.450000
Measurement pX
8.83
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Chbb:THOM rat
Organs/Tissues
hippocampus
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[H3]-flunitrazepam
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Measurement Parameter
Bmax
Unit
pmol/g
Quantitative value
55.9
Deviation
22
Concomitants: Compound RN
754371
Concomitants: Compound name
Diazepam
Concomitants: Compound role
NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Synaptosome: membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
1.2
Measurement pX
8.92
Concomitants: Compound RN
754371; 759557
Concomitants: Compound name
[3H]-Diazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p18507 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
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Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
4.6
Deviation
0.770000
Measurement pX
8.34
Concomitants: Compound RN
8080906; 4763661
Concomitants: Compound name
L-655,708; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p31644 + p18505 + p18507
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1.8
Deviation
0.0800000
Measurement pX
8.74
Concomitants: Compound RN
8080906; 4763661
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound name
L-655,708; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p18507
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Serine,205,Threonine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.8
Deviation
1
Measurement pX
8.42
Concomitants: Compound RN
8080906; 4763661
Concomitants: Compound name
L-655,708; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p18507
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Valine,239,Isoleucine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
160
Deviation
41
Measurement pX
6.8
Concomitants: Compound RN
8080906; 4763661
Concomitants: Compound name
L-655,708; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p18507
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Serine,232,Threonine,Substitution,2,Valine,239,Isoleucine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.1
Deviation
1.70000
Measurement pX
8.51
Concomitants: Compound RN
8080906; 4763661
Concomitants: Compound name
L-655,708; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
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Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p18505 + p18507
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Mutated:Substitution,1,Threonine,239,Serine,Substitution,2,Isoleucine,246,Valine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
24
Deviation
8
Measurement pX
7.62
Concomitants: Compound RN
8080906; 4763661
Concomitants: Compound name
L-655,708; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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291 of 976
Quantitative value
8.9
Deviation
0.900000
Measurement pX
8.05
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Glycine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
13
Deviation
3
Measurement pX
7.89
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
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Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Serine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
15
Deviation
2
Measurement pX
7.82
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Cysteine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Unit
nM
Quantitative value
48
Deviation
5
Measurement pX
7.32
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Glutamic acid + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
4.4
Deviation
1.60000
Measurement pX
8.36
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
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Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Glutamine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
79
Deviation
13
Measurement pX
7.1
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Leucine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
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Measurement Parameter
Ki
Unit
nM
Quantitative value
20
Deviation
3
Measurement pX
7.7
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Phenylalanine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
10
Deviation
1
Measurement pX
8
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
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+ gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
298 of 976
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Tyrosine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
6.1
Deviation
2.20000
Measurement pX
8.21
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Arginine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
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300 of 976
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
24
Deviation
5
Measurement pX
7.62
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,117,Tryptophan + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
20
Deviation
4
Measurement pX
7.7
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human];+GABA-A receptor (Gamma 2S) [human]
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Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p28472 + p31644
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.19
Measurement pX
8.66
Concomitants: Compound RN
6182319; 6182319
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-4513
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p28472
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Mutated:Substitution,1,Lysine,105,Arginine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
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Substance Name
flunitrazepam
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
26
Measurement pX
4.55
Concomitants: Compound RN
6182319; 6182319
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-4513
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-4 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-4; gabra4; gamma-aminobutyric acid receptor subunit alpha-4 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p28472 + p48169
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-4 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-4 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
2
Measurement pX
1
Concomitants: Compound RN
6182319; 6182319
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-4513
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-6 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba-a receptor (gamma 2s)
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Target Uniprot ID
p28472 + q16445
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-6 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-6 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
6
Measurement pX
1
Concomitants: Compound RN
6182319; 6182319
Concomitants: Compound name
Ro 15-4513; [3H]-Ro 15-4513
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p28472 + p31644
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
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Measurement Parameter
% Stimulation
Unit
%
Quantitative value
147.2
Deviation
1.70000
Concomitants: Compound RN
24140623; 12471441; 3568341; 906818; 9186271
Concomitants: Compound name
Gentamycin; Streptomycin; Sodium pyruvate; GABA; Penicillin
Concomitants: Compound role
COM; COM; COM; AGO; COM
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p28472
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Mutated:Substitution,1,Lysine,105,Arginine
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
5
Deviation
3
Concomitants: Compound RN
24140623; 12471441; 3568341; 906818; 9186271
Concomitants: Compound name
Gentamycin; Streptomycin; Sodium pyruvate; GABA; Penicillin
Concomitants: Compound role
COM; COM; COM; AGO; COM
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
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Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
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Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Deviation
0.800000
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
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Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Deviation
0.800000
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
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Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
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Quantitative value
3.9
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.9
Deviation
0.800000
Measurement pX
8.41
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit alpha-6 [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit alpha-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s)
Target Uniprot ID
q16445 + p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-6 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-6 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
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Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
5000
Measurement pX
5.3
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-11 M
Measurement Parameter
EC50
Unit
nM
Quantitative value
1.9
Measurement pX
8.72
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
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Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-11 M
Measurement Parameter
% Max
Unit
%
Measurement Object
% of maximum potentiation
Quantitative value
104
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Lysine
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-11 M
Measurement Parameter
nH
Quantitative value
0.9
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Deviation
0.0800000
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Biological Species/NCBI ID
DBA 2 mouse
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
µmol/kg
Measurement Object
inhibition against clonic seizures
Quantitative value
0.22
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 319 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Biological Species/NCBI ID
DBA 2 mouse
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
µmol/kg
Measurement Object
Dose required to inhibit tonic seizures
Quantitative value
0.18
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 320 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Biological Species/NCBI ID
Swiss Webster mouse
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
µmol/kg
Measurement Object
inhibition of pentylenetetrazol induced clonic seizure
Quantitative value
0.29
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Concomitants: Compound RN
135492
Concomitants: Compound name
Pentetrazole
Concomitants: Compound role
IAV
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 321 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Rotarod test
Biological Species/NCBI ID
Swiss Webster mouse
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ED50
Unit
µmol/kg
Measurement Object
Induction of falling of the animals from the rota-rod
Quantitative value
8.7
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 322 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
cerebellum
Cell Fraction
Microsome: membrane (P3 fraction)
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
5
Deviation
2
Measurement pX
8.3
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 323 of 976
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cell Fraction
Microsome: membrane (P3 fraction)
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
7
Deviation
2
Measurement pX
8.15
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 324 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
spinal cord
Cell Fraction
Microsome: membrane (P3 fraction)
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Qualitative value
ND
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 325 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63079 + p18508 + p62813
Target PDB ID
2pr9
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Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3
Deviation
0.0300000
Measurement pX
8.52
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 326 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p63079 + p18508 + p23576
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
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Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1.46
Deviation
0.560000
Measurement pX
8.84
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 327 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p63079 + p18508 + p20236
Target PDB ID
2pr9 + 4tk1; 4tk2; 4tk3; 4tk4; 4u90
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1.66
Deviation
0.780000
Measurement pX
8.78
Concomitants: Compound RN
702691; 4763661
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 328 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p63079 + p19969 + p18508
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1.51
Deviation
0.300000
Measurement pX
8.82
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 329 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-6 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
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Target Uniprot ID
p63079 + p30191 + p18508
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-6 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
10000
Measurement pX
1
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
De Sarro, Giovambattista; Carotti, Angelo; Campagna, Francesco; McKernan, Ruth; Rizzo, Milena; Falconi, Umberto; Palluotto, Fausta; Giusti, Pietro; Rettore, Cristina; De Sarro, Angela; Pharmacology Biochemistry and Behavior; vol. 65; nb. 3; (2000); p. 475 - 487, View in Reaxys 330 of 976
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p62813 + p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
11.6
Measurement pX
7.94
Concomitants: Compound RN
906818; 906818
Concomitants: Compound name
GABA; GABA
Concomitants: Compound role
AGO; AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Proline,304,Alanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
5.8
Deviation
1.90000
Measurement pX
8.24
Concomitants: Compound RN
906818
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333 of 976
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Valine,306,Alanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
122
Deviation
6
Measurement pX
6.91
Concomitants: Compound RN
906818; 906818
Concomitants: Compound name
GABA; GABA
Concomitants: Compound role
AGO; AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
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Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,307,Serine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
6.8
Deviation
0.300000
Measurement pX
8.17
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Alanine,307,Serine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
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Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
%
Unit
%
Measurement Object
Efficacy
Quantitative value
185
Deviation
1.42000
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Proline,304,Alanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
%
Unit
%
Measurement Object
Efficacy
Quantitative value
278
Deviation
17
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
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Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Valine,306,Alanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
%
Unit
%
Measurement Object
Efficacy
Quantitative value
375
Deviation
65
Concomitants: Compound RN
906818; 906818
Concomitants: Compound name
GABA; GABA
Concomitants: Compound role
AGO; AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Alanine
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Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
629
Deviation
103.300
Measurement pX
6.2
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Valine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
587
Deviation
83
Measurement pX
6.23
Concomitants: Compound RN
6182319; 702691
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Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Glutamic acid
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
5000
Measurement pX
5.3
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Glutamine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
5000
Measurement pX
5.3
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Methionine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1660
Deviation
770
Measurement pX
5.78
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
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Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Lysine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
4390
Deviation
850
Measurement pX
5.36
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Tyrosine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
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Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1380
Deviation
300
Measurement pX
5.86
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Arginine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
5000
Measurement pX
5.3
Concomitants: Compound RN
6182319; 702691
Concomitants: Compound name
[3H]-Ro 15-4513; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
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Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Lysine
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-11 M
Measurement Parameter
EC50
Unit
nM
Quantitative value
813
Measurement pX
6.09
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Mutated:Substitution,1,Histidine,129,Lysine
Target Species (Bioactivity)
human
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-11 M
Measurement Parameter
% Max
Unit
%
Measurement Object
% of maximum potentiation
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Quantitative value
91
Deviation
6.70000
Substance Effect
Sedative
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Spontaneous activity
Biological Species/NCBI ID
C57BL 6 mouse
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
of times a mouse interrupted the photosensor beams
Quantitative value
56
Deviation
14
Statistical sgnificance (pvalue)
0.01
Harris, Danni L.; Delorey, Timothy M.; He, Xiaohui; Cook, James M.; Loew, Gilda H.; European Journal of Pharmacology; vol. 401; nb. 3; (2000); p. 271 - 287, View in Reaxys 348 of 976
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Valine,306,Alanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
EC50
Unit
nM
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349 of 976
350 of 976
Quantitative value
40.5
Deviation
7.70000
Statistical sgnificance (pvalue)
0.01
Measurement pX
7.39
Concomitants: Compound RN
906818; 906818
Concomitants: Compound name
GABA; GABA
Concomitants: Compound role
AGO; AGO
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
11
Measurement Parameter
Ki
Unit
nM
Quantitative value
10
Deviation
0.400000
Measurement pX
8
Concomitants: Compound RN
4264456; 8951860
Concomitants: Compound name
PK 11195; [3H]-CB 34
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
thalamus
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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351 of 976
352 of 976
Unit
nM
Quantitative value
2.9
Deviation
0.200000
Measurement pX
8.54
Concomitants: Compound RN
6182319; 6158829
Concomitants: Compound name
[3H]-Ro 15-4513; Bretazenil
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-4 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-4; gabra4; gamma-aminobutyric acid receptor subunit alpha-4
Target Uniprot ID
p18508 + p28471
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-4 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-4 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
thalamus
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Qualitative value
ND
Concomitants: Compound RN
6182319; 6158829
Concomitants: Compound name
[3H]-Ro 15-4513; Bretazenil
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-4 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-4; gabra4; gamma-aminobutyric acid receptor subunit alpha-4 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p28472 + p48169
Target PDB ID
4cof
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-4 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-4 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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353 of 976
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
12
Deviation
1
Measurement pX
1
Concomitants: Compound RN
6182319; 6158829
Concomitants: Compound name
[3H]-Ro 15-4513; Bretazenil
Concomitants: Compound role
RAD; NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
thalamus
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
11
Deviation
2
Measurement pX
1
Concomitants: Compound RN
6182319; 6158829
Concomitants: Compound name
[3H]-Ro 15-4513; Bretazenil
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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354 of 976
Concomitants: Compound role
RAD; NSB
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
sleep latency in rats placed on sawdust
Quantitative value
19
Deviation
2.90000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 355 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
sleep latency in rats placed on sawdust
Quantitative value
16.9
Deviation
4.20000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
210/493
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Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 356 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
sleep latency in rats placed on sawdust
Quantitative value
17.2
Deviation
3
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 357 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
sleep latency in rats placed on sawdust
Quantitative value
11.5
Deviation
1.70000
Statistical sgnificance (pvalue)
0.05
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
211/493
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Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 358 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
sleep latency in rats placed on a grid suspended over water
Quantitative value
34.6
Deviation
4.50000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 359 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
sleep latency in rats placed on a grid suspended over water
Quantitative value
33.6
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
212/493
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Deviation
6.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 360 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
sleep latency in rats placed on a grid suspended over water
Quantitative value
18.6
Deviation
2.90000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 361 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
213/493
2018-04-14 18:48:42
Measurement Object
sleep latency in rats placed on a grid suspended over water
Quantitative value
16.2
Deviation
4.30000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 362 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
32.5
Deviation
2.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 363 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
214/493
2018-04-14 18:48:42
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
31.6
Deviation
2.20000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 364 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
34.3
Deviation
1.30000
Statistical sgnificance (pvalue)
0.05
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 365 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
215/493
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Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
38.3
Deviation
1.20000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 366 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
39.4
Deviation
2.60000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 367 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
216/493
2018-04-14 18:48:42
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
37.3
Deviation
2.50000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 368 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
38.4
Deviation
1.80000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 369 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
217/493
2018-04-14 18:48:42
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
38.8
Deviation
1.80000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 370 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
39.6
Deviation
2.20000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 371 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
218/493
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Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
38.4
Deviation
2.60000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 372 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
40.4
Deviation
0.800000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 373 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
219/493
2018-04-14 18:48:42
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
36.7
Deviation
1.20000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 374 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
34.4
Deviation
2.10000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 375 of 976
Substance Effect
Hypnotic
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
220/493
2018-04-14 18:48:42
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
36.9
Deviation
2
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 376 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
36.8
Deviation
2.80000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
221/493
2018-04-14 18:48:42
377 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
38.7
Deviation
1.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 378 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
33.6
Deviation
3
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
222/493
2018-04-14 18:48:42
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 379 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
38.2
Deviation
1.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 380 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
32.9
Deviation
3.20000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
223/493
2018-04-14 18:48:42
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 381 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
39
Deviation
1.10000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 382 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
30.2
Deviation
3.30000
Concomitants: Compound RN
24141036
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
224/493
2018-04-14 18:48:42
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 383 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
36.4
Deviation
1.80000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 384 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
38.7
Deviation
1.60000
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
225/493
2018-04-14 18:48:42
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 385 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
37.2
Deviation
1.20000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 386 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
26.7
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
226/493
2018-04-14 18:48:42
Deviation
2.70000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 387 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
26.7
Deviation
1.10000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 388 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
227/493
2018-04-14 18:48:42
Quantitative value
31.1
Deviation
1.70000
Statistical sgnificance (pvalue)
0.05
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 389 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
31.5
Deviation
2.50000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 390 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
228/493
2018-04-14 18:48:42
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
24.5
Deviation
2.30000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 391 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
24.6
Deviation
2
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 392 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
229/493
2018-04-14 18:48:42
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
31.2
Deviation
1.20000
Statistical sgnificance (pvalue)
0.05
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 393 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
33.6
Deviation
1.50000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 394 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
230/493
2018-04-14 18:48:42
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
19.5
Deviation
2.80000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 395 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
25.8
Deviation
1.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 396 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
231/493
2018-04-14 18:48:42
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
26.8
Deviation
2.10000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 397 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
28.1
Deviation
1.50000
Statistical sgnificance (pvalue)
0.05
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
232/493
2018-04-14 18:48:42
398 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
24.8
Deviation
0.600000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 399 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
25.7
Deviation
2.50000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
233/493
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Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 400 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
20.5
Deviation
1.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 401 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
24.5
Deviation
1.60000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
234/493
2018-04-14 18:48:42
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 402 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
21.6
Deviation
2.60000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 403 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
18.2
Deviation
2.20000
Concomitants: Compound RN
24141036
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
235/493
2018-04-14 18:48:42
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 404 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
23.2
Deviation
2.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 405 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep for rats placed on sawdust
Quantitative value
24.8
Deviation
1.50000
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
236/493
2018-04-14 18:48:42
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 406 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep in rats placed on a grid suspended over water
Quantitative value
23.3
Deviation
1.50000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 407 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep in rats placed on a grid suspended over water
Quantitative value
28.8
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
237/493
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Deviation
1.50000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 408 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep in rats placed on a grid suspended over water
Quantitative value
29.8
Deviation
1.60000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 409 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
238/493
2018-04-14 18:48:42
Measurement Parameter
Time
Unit
minute
Measurement Object
non-REM sleep in rats placed on a grid suspended over water
Quantitative value
31.1
Deviation
2.50000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 410 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
43.3
Deviation
4.70000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 411 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
239/493
2018-04-14 18:48:42
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
46.9
Deviation
2.70000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 412 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
44.4
Deviation
4.90000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 413 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
240/493
2018-04-14 18:48:42
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
43.5
Deviation
3.40000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 414 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
30.1
Deviation
1.10000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 415 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
241/493
2018-04-14 18:48:42
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
29.5
Deviation
0.800000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 416 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
32.3
Deviation
1.60000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 417 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
242/493
2018-04-14 18:48:42
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
29.9
Deviation
1.30000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 418 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
304.3
Deviation
21.9000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 419 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
243/493
2018-04-14 18:48:42
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
282.8
Deviation
13.9000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 420 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
311.1
Deviation
20.8000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 421 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
244/493
2018-04-14 18:48:42
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on sandwust
Quantitative value
321.5
Deviation
19.1000
Statistical sgnificance (pvalue)
0.05
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 422 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
273.5
Deviation
7.80000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 423 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
245/493
2018-04-14 18:48:42
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
300.1
Deviation
13.9000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 424 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
296.3
Deviation
8
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 425 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
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Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
non-REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
335.1
Deviation
4.10000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 426 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
14.4
Deviation
1
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 427 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
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Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
14.4
Deviation
0.900000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 428 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
13
Deviation
0.700000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys
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429 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
12.1
Deviation
0.500000
Statistical sgnificance (pvalue)
0.01
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 430 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
17.3
Deviation
1.30000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
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Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 431 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
17.9
Deviation
1.50000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 432 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
15.6
Deviation
1.20000
Concomitants: Compound RN
24141036
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Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 433 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Time
Unit
second
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
14.5
Deviation
1.10000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 434 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
115
Deviation
10
Concomitants: Compound RN
24141036
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Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 435 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
126.8
Deviation
6.90000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 436 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
112.9
Deviation
7.90000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
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Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 437 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on sandwust
Quantitative value
91.1
Deviation
10.3000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 438 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
49.5
Deviation
6.50000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
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Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 439 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
57.3
Deviation
8
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 440 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
42.8
Deviation
2.90000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 441 of 976
Substance Effect
Hypnotic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Sedation
Biological Species/NCBI ID
Wistar rat
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
5 mg/kg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Number
Measurement Object
REM sleep episode duration in rats placed on a grid suspended over water
Quantitative value
65.4
Deviation
10.2000
Concomitants: Compound RN
24141036
Concomitants: Compound name
Pentobarbital sodium
Concomitants: Compound role
ANA
Shinomiya, Kazuaki; Shigemoto, Yuki; Omichi, Junji; Utsu, Yoshiaki; Mio, Mitsunobu; Kamei, Chiaki; Psychopharmacology; vol. 173; nb. 1-2; (2004); p. 203 - 209, View in Reaxys 442 of 976
Substance Effect
Behavioural Symptoms
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Target : Long-Evans ratBioassay : GHB: α-hydroxybutyrate title comp. was administered 1 week before, during (1000 mg/kg i.p. for 1 week) and 2 weeks after GHB treatment; motor performance (latency to fall) was recorded at both low (8 rpm) and high (32 rpm) speeds; rotorod assay
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.0100000 mg/kg
Substance Route of Adm.
intraperitoneal administration
Qualitative Results
title comp. dose-dependently decreased motor performance at both speeds, but was 4.6-15 fold more potent at high speed; GHB treatment did not alter potency of title comp.
Measurement Parameter
Qualitative
Smith, Mark A.; Gergans, Samantha R.; Lyle, Megan A.; European Journal of Pharmacology; vol. 552; nb. 1-3; (2006); p. 83 - 89, View in Reaxys 443 of 976
Target Name
UDP-glucuronosyltransferase [human]
Target Synonyms
udp-glucuronosyltransferase 1a9; udp-glucuronosyltransferase; udp-glucuronosyltransferase variant; ugt1a1; ugt1a10; ugt1a3; ugt1a4; ugt1a6; ugt1a7; ugt1a9; ugt1g; ugt1j; ugt2a1; ugt2b7; ugt3a1
Target Uniprot ID
a0a024rdc9; a3kme6; b4ddy2; b4dzz5; b5mct4; b7z3n0; b7z8q8; d6rfw5; d6rh08; e9pd17; i6u9t8; i6ujg0; i6v2c0; i6vbt8; i6vbu3; i6vbu7; i6ve22; i6ve27; q0vaj1; q13406; q13407; q4r0z5; q4r0z7; q53e75; q53gq9; q53gu2; q5dsz7; q5dsz8; q5dsz9; q5dt01; q5dt02; q5dt03; q5k632; q5k672; q5k673; q5k674; q5qte0; q5qte1; q5qte2; q5qte4; q75xt5; q8wuq4; q8wx86; q8wx88; q8wx89; q8wx90; q9h3f9; q9h3g0; q9h3g1; q9h3g2
Target, Subunit, Species
UDP-glucuronosyltransferase [human]
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444 of 976
Target Mutant/Chimera Details
UDP-glucuronosyltransferase [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0 mM
Measurement Parameter
Ki
Unit
mM
Quantitative value
0.19
Deviation
0.0300000
Measurement pX
3.72
Metabolite RN
7452311
Metabolite name
Zidovudine glucuronide
Concomitants: Compound RN
3595791; 78881
Concomitants: Compound name
Zidovudine; Uridine 5'-diphosphoglucuronic acid
Concomitants: Compound role
SUB; COM
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : gaba-A, Benzodiazepine (Central) receptor bindingTarget : Receptor source - bovine cortical membranesBioassay : [00100] Experiment 6-Bioactivity of Infused Agents-Pharmacology Activity Test [00101] It was demonstrated that introduced drugs and drug-like agents can maintain their pharmacological activity during their residence in the biological materials. The following procedures were employed and the following
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
Flunitrazepam, a hypnotic, demonstrated specific binding in the benzodiazepine central assay
Measurement Parameter
Qualitative
Patent; American Moxie LLC; US6660310; (2003); (B2) English, View in Reaxys 445 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Target : embryos from 14-day-old C57B1/6CR mouseBioassay : non specific binding was detn. in presence of 10 μM Ro15-1788 coverslips contg. cortical cultured neurons pretreated with pentobarbital (200 μM, 5-days), or not (control) removed from the tissue culture medium rinsed at room temp. in HEPES-buffered saline, incubated with (3H)title comp. for 30 min at 24 deg C
Substance RN
702691View in Reaxys
Substance Name
31870
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Substance Dose
1 nM
Qualitative Results
specific binding: control 141 fmol/mg protein; in cells pretreated with 200 μM pentobarbital for 5 days 154 fmol/mg protein; chronic pretreatment with pentobarbital did not change basal title comp. binding to intact cortical neurons
Measurement Parameter
Qualitative
Yu; Ticku; Journal of Pharmacology and Experimental Therapeutics; vol. 275; nb. 3; (1995); p. 1442 - 1446, View in Reaxys 446 of 976
Substance Effect
Anticonvulsant
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : seizure stageBioassay : AD: afterdischarge bipolar electrodes implanted into right amygdala of rats; stimulation: 60 Hz, 1 ms and 1 s duration at intensity sufficient to induce AD; stimulation performed 1 h after title comp. administration; amygdaloid kindling classified by Racine method
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.500000 mg/kg
Substance Route of Adm.
oral administration
Measurement Parameter
ED50
Unit
mg/kg
Quantitative value
0.89
Fukinaga, Masafumi; Ishizawa, Keisuke; Kamei, Chiaki; Pharmacology; vol. 57; nb. 5; (1998); p. 233 - 241, View in Reaxys 447 of 976
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.2
Measurement pX
8.66
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
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Concomitants: Compound role
NSB; RAD
Atack, John R.; Smith, Alison J.; Emms, Frances; McKernan, Ruth M.; Neuropsychopharmacology; vol. 20; nb. 3; (1999); p. 255 - 262, View in Reaxys 448 of 976
Target Name
Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p47869
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.5
Measurement pX
8.6
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Atack, John R.; Smith, Alison J.; Emms, Frances; McKernan, Ruth M.; Neuropsychopharmacology; vol. 20; nb. 3; (1999); p. 255 - 262, View in Reaxys 449 of 976
Target Name
Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p34903
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement Parameter
Ki
Unit
nM
Quantitative value
4.5
Measurement pX
8.35
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Atack, John R.; Smith, Alison J.; Emms, Frances; McKernan, Ruth M.; Neuropsychopharmacology; vol. 20; nb. 3; (1999); p. 255 - 262, View in Reaxys 450 of 976
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [human]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p31644
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.1
Measurement pX
8.68
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Atack, John R.; Smith, Alison J.; Emms, Frances; McKernan, Ruth M.; Neuropsychopharmacology; vol. 20; nb. 3; (1999); p. 255 - 262, View in Reaxys 451 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Swiss Webster mouse
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Route of Adm.
intraperitoneal administration
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
mg/kg
Quantitative value
0.2 - 0.3
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
OCV
Atack, John R.; Smith, Alison J.; Emms, Frances; McKernan, Ruth M.; Neuropsychopharmacology; vol. 20; nb. 3; (1999); p. 255 - 262, View in Reaxys 452 of 976
453 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.300000 nM
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.3
Deviation
0.500000
Measurement pX
8.64
Concomitants: Compound RN
702691; 702691
Concomitants: Compound name
Flunitrazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Mus musculus
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.300000 nM
Measurement Parameter
Ki
Unit
nM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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454 of 976
455 of 976
Quantitative value
2.3
Deviation
0.500000
Measurement pX
8.64
Concomitants: Compound RN
702691; 702691
Concomitants: Compound name
Flunitrazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.300000 nM
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
ID50
Unit
µmol/kg
Quantitative value
1.69
Deviation
0.970000
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Brain biochemistry : Others
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.320000 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Concentration
Unit
µmol/kg
Measurement Object
of [3H]-flunitrazepam
Quantitative value
0.13
Deviation
0.0200000
Concomitants: Compound RN
702691
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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456 of 976
457 of 976
458 of 976
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Brain biochemistry : Others
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
3.20000 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Concentration
Unit
µmol/kg
Measurement Object
of [3H]-flunitrazepam
Quantitative value
1.4
Deviation
1
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Bioassay Name
Brain biochemistry : Others
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
32 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
Concentration
Unit
µmol/kg
Measurement Object
of [3H]-flunitrazepam
Quantitative value
6.3
Deviation
4
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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459 of 976
460 of 976
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.0318000 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
7
Deviation
7
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.318000 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
12
Deviation
8
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
3.18000 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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461 of 976
462 of 976
Substance Dosing Regimen
Single
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
78
Deviation
8
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
9.55000 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
85
Deviation
1
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In Vivo: Binding
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
31.8000 µmol/kg
Substance Route of Adm.
intraperitoneal administration
Substance Dosing Regimen
Single
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
92
Deviation
4
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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463 of 976
464 of 976
Concomitants: Compound RN
702691
Concomitants: Compound name
[3H]-Flunitrazepam
Concomitants: Compound role
OCV
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18507 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
3
Deviation
0.800000
Measurement pX
8.52
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Mutated:Substitution,1,Phenylalanine,116,Tyrosine
Target Species (Bioactivity)
human
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465 of 976
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
502
Deviation
2
Measurement pX
6.3
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Mutated:Substitution,1,Phenylalanine,116,Leucine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
7.2
Deviation
3.20000
Measurement pX
8.14
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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466 of 976
467 of 976
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Mutated:Substitution,1,Phenylalanine,116,Isoleucine
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
4.5
Deviation
0.700000
Measurement pX
8.35
Concomitants: Compound RN
702691
Concomitants: Compound name
Flunitrazepam
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Mutated:Substitution,1,Phenylalanine,116,Tryptophan
Target Species (Bioactivity)
human
Target Transfection
Transfected
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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468 of 976
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
18
Deviation
4.30000
Measurement pX
7.74
Concomitants: Compound RN
702691
Concomitants: Compound name
Flunitrazepam
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Mutated:Substitution,1,Phenylalanine,116,Tryptophan
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
nH
Quantitative value
1
Concomitants: Compound RN
702691
Concomitants: Compound name
Flunitrazepam
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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469 of 976
470 of 976
Concomitants: Compound role
NSB
Target Name
GABA-A receptor (Gamma 1) [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba-a receptor (gamma 1) + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p47870 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 1) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 1) [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
3H-Flunitrazepam
Measurement Parameter
Kd
Unit
µM
Quantitative value
37.74
Deviation
5.07000
Measurement pX
4.42
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18508 + p63138 + p62813
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance RN
702691View in Reaxys
Substance Name
2
Measurement Parameter
Ki
Unit
nM
Quantitative value
3
Measurement pX
8.52
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Sigel, Erwin; Schaerer, Martin T.; Buhr, Andreas; Baur, Roland; Molecular Pharmacology; vol. 54; nb. 6; (1998); p. 1097 1105, View in Reaxys 471 of 976
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Phenylalanine,115,Tyrosine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
2
Measurement Parameter
Ki
Unit
nM
Quantitative value
500
Measurement pX
6.3
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Sigel, Erwin; Schaerer, Martin T.; Buhr, Andreas; Baur, Roland; Molecular Pharmacology; vol. 54; nb. 6; (1998); p. 1097 1105, View in Reaxys
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Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Threonine,233,Valine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
2
Measurement Parameter
Ki
Unit
nM
Quantitative value
36
Measurement pX
7.44
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Sigel, Erwin; Schaerer, Martin T.; Buhr, Andreas; Baur, Roland; Molecular Pharmacology; vol. 54; nb. 6; (1998); p. 1097 1105, View in Reaxys 473 of 976
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Mutated:Substitution,1,Methionine,168,Leucine + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
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Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
2
Measurement Parameter
Ki
Unit
nM
Quantitative value
8
Measurement pX
8.1
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Sigel, Erwin; Schaerer, Martin T.; Buhr, Andreas; Baur, Roland; Molecular Pharmacology; vol. 54; nb. 6; (1998); p. 1097 1105, View in Reaxys 474 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+GABA-A receptor (Gamma 2S) [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild + GABA-A receptor (Gamma 2S) [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
9.9
Deviation
0.800000
Measurement pX
8
Concomitants: Compound RN
767925
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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475 of 976
476 of 976
Concomitants: Compound name
Flurazepam
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+GABA-A receptor (Gamma 2S) [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p63138 + p62813
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild + GABA-A receptor (Gamma 2S) [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/mg
Quantitative value
0.65
Deviation
0.0700000
Concomitants: Compound RN
767925
Concomitants: Compound name
Flurazepam
Concomitants: Compound role
NSB
Target Name
GABA-A receptor [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba-a receptor + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p63138
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Chimera + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
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Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
11.3
Deviation
1.70000
Measurement pX
7.95
Concomitants: Compound RN
767925
Concomitants: Compound name
Flurazepam
Concomitants: Compound role
NSB
Target Name
GABA-A receptor [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba-a receptor + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p63138
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Chimera + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/mg
Quantitative value
0.34
Deviation
0.0300000
Concomitants: Compound RN
767925
Concomitants: Compound name
Flurazepam
Concomitants: Compound role
NSB
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479 of 976
Target Name
GABA-A receptor [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba-a receptor + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p63138
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Chimera + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
13.3
Deviation
3.50000
Measurement pX
7.88
Concomitants: Compound RN
767925
Concomitants: Compound name
Flurazepam
Concomitants: Compound role
NSB
Target Name
GABA-A receptor [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba-a receptor + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p63138
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Chimera + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
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480 of 976
481 of 976
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/mg
Quantitative value
0.57
Deviation
0.170000
Concomitants: Compound RN
767925
Concomitants: Compound name
Flurazepam
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Tyrosine,236,Alanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Qualitative value
ND
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit
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gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
482 of 976
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Tyrosine,236,Glutamine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
100
Measurement pX
7
Concomitants: Compound RN
606417; 774694
Concomitants: Compound name
Muscimol; [3H]-Muscimol
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Tyrosine,236,Phenylalanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
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483 of 976
484 of 976
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Qualitative value
ND
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Threonine,233,Alanine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Qualitative value
ND
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18505 + p18507 + p14867
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485 of 976
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
9.3
Deviation
1.40000
Measurement pX
8.03
Concomitants: Compound RN
6182319
Concomitants: Compound name
Ro 15-4513
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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487 of 976
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
15.3
Deviation
2.30000
Measurement pX
7.82
Concomitants: Compound RN
6182319
Concomitants: Compound name
Ro 15-4513
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-6 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p28472 + q16445 + p18507
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-6 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Qualitative value
ND
Concomitants: Compound RN
6182319
Concomitants: Compound name
Ro 15-4513
Concomitants: Compound role
NSB
Target Name
GABA-A receptor [taurine cattle]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor [taurine cattle]:Wild
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Target Species (Bioactivity)
taurine cattle
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
fluni
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
100
Deviation
5.20000
Measurement pX
6.28
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [taurine cattle]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
fluni
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.17
Deviation
0.200000
Measurement pX
8.66
Concomitants: Compound RN
754371; 4763661
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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490 of 976
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Tyrosine,236,Glutamine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
43
Deviation
6
Measurement pX
7.37
Concomitants: Compound RN
702691
Concomitants: Compound name
Flunitrazepam
Concomitants: Compound role
NSB
Target Name
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p18508 + p63138
Target PDB ID
2pr9
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Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Mutated:Substitution,1,Tyrosine,236,Glutamine + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Bmax
Unit
fmol/mg protein
Qualitative value
#
Quantitative value
400
Concomitants: Compound RN
702691
Concomitants: Compound name
Flunitrazepam
Concomitants: Compound role
NSB
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : oocytes expressing human α3, α1 and α2L GABAA receptor subunits; effect of title comp. on pentobarbitone-, 5α-pregnan-3α-ol-20-one-, and propofolevoked currents mediated by the recombinant receptors in vitro; oocytes voltage-clamped at -60 mV; two-electrode voltage-clamp mode; continuously superfused (7-10 ml/min) with frog Ringer soln.; title comp., pentobarbitone, 5α-pregnan-3α-ol-20-one, and propofol applied by the superfusion system
Biological Species/NCBI ID
Xenopus laevis
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.300000 µM
Qualitative Results
title comp. enhanced responses to pentobarbitone, propofol, and 5α-pregnan-3α-ol-20-one
Measurement Parameter
Qualitative
Belelli, Delia; Callachan, Helen; Hill-Venning, Claire; Peters, John A.; Lambert, Jeremy J.; British Journal of Pharmacology; vol. 118; nb. 3; (1996); p. 563 - 576, View in Reaxys 492 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : membranes preincubated with 50 nmol/l des-methyl flunitrazepam for 30 min at 4 deg C and exposed to UV light (365 nm, 50-60 Hz, 200-220 V) for 1 h at 4 deg C; GABA: α-amino-
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butyric acid; BZ: benzodiazepine binding of <3H>title comp. to BZ binding site of GABAA receptor after photoincorporation of des-methyl flunitrazepam studied by saturation assay; incubation for 30 min at 20 deg C; radioactivity measured by scintillation counting Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 nM
Qualitative Results
Bmax = 22.1 percent, Kd not detected (table)
Measurement Parameter
Qualitative
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 493 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : membranes preincubated with 50 nmol/l Ro 15-4513 (partial inverse agonist) for 30 min at 4 deg C and exposed to UV light (365 nm, 50-60 Hz, 200-220 V) for 1 h at 4 deg C; GABA: α-aminobutyric acid; BZ: benzodiazepine binding of <3H>title comp. to BZ binding site of GABAA receptor before and after photoincorporation of Ro 15-4513 studied by saturation assay; incubation for 30 min at 20 deg C; radioactivity measured by scintillation
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 nM
Qualitative Results
binding of <3H>title comp. was reduced after photoincorporation of Ro 15-4513 by > 68 percent (Kd: 5.33 and 2.93 nmol/l, Bmax: 95.1 and 12.9 percent before and after photoincorporation, resp.) (figure, table)
Measurement Parameter
Qualitative
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 494 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : membranes preincubated with 50 nmol/l title comp. for 30 min at 4 deg C and exposed to UV light (365 nm, 50-60 Hz, 200-220 V) for 1 h at 4 deg C; GABA: α-aminobutyric acid; BZ: benzodiazepine affinity of title comp. to BZ binding site of GABAA receptor before and after photoincorporation of title comp. studied by competition assay using <3H>Ro 15-1788 as radioligand
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.300000 nM
Qualitative Results
Ki: 4.2 and 230.0 nmol/l before and after title comp. photoincorporation, resp. (table)
Measurement Parameter
Qualitative
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 495 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : membranes preincubated with 50 nmol/l title comp. for 30 min at 4 deg C and exposed to UV light (365 nm, 50-60 Hz, 200-220 V) for 1 h at 4 deg C; GABA: α-aminobutyric acid; BZ: benzodiazepine binding of <3H>title comp. to BZ binding site of GABAA receptor before and after photoincorporation of title comp. studied in presence of GABA (1 mmol/l)
Substance RN
702691View in Reaxys
Substance Name
31870
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Substance Dose
3 nM
Qualitative Results
binding of <3H>title comp. in presence of GABA: 156.3 percent before and no binding after title comp. photoincorporation resp. (table)
Measurement Parameter
Qualitative
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 496 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : membranes preincubated with 50 nmol/l title comp. (full agonist) for 30 min at 4 deg C and exposed to UV light (365 nm, 50-60 Hz, 200-220 V) for 1 h at 4 deg C; GABA: α-aminobutyric acid; BZ: benzodiazepine binding of <3H>title comp. to BZ binding site of GABAA receptor before and after photoincorporation of title comp. studied by saturation assay; incubation for 30 min at 20 deg C; radioactivity measured by scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 nM
Qualitative Results
binding of <3H>title comp. was reduced after photoincorporation of title comp. by 84.1 percent (Kd: 5.33 and 6.06 nmol/l, Bmax: 95.1 and 9.4 percent before and after photoincorporation, resp.) (figure, table)
Measurement Parameter
Qualitative
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 497 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : GABA: α-aminobutyric acid; BZ: benzodiazepine affinity of title comp. for BZ binding site of GABAA receptor studied using radioligand <3H>title comp.
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.6
Measurement pX
8.59
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 498 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : GABA: α-aminobutyric acid; BZ:benzodiazepine affinity of title comp. for BZ binding site of GABAA receptor studied using radioligand <3H>CGS 8216
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
4.7
Measurement pX
8.33
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys
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Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : GABA: α-aminobutyric acid; BZ:benzodiazepine affinity of title comp. for BZ binding site of GABAA receptor studied using radioligand <3H>Ro 15-1788
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.9
Measurement pX
8.23
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 500 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : GABA: α-aminobutyric acid; BZ:benzodiazepine affinity of title comp. for BZ binding site of GABAA receptor studied using radioligand <3H>Ro 15-4513
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
3.2
Measurement pX
8.49
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 501 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : membranes preincubated with des-methyl, des-nitro flunitrazepam for 30 min at 4 deg C and exposed to UV light (365 nm, 50-60 Hz, 200-220 V) for 1 h at 4 deg C; GABA: α-aminobutyric acid; BZ: benzodiazepine binding of <3H>title comp. to BZ binding site of GABAA receptor after photoincorporation of des-methyl, des-nitro flunitrazepam studied by saturation assay; incubation for 30 min at 20 deg C; radioactivity measured by scintillation
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
3.4
Measurement pX
8.47
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 502 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : cell membranes expressing human α1α3α2 GABAA receptorBioassay : membranes preincubated with 500 nmol/l chlordiazepoxide for 30 min at 4 deg C and exposed to UV light (365 nm, 50-60 Hz, 200-220 V) for 1 h at 4 deg C; GABA: α-aminobutyric acid; BZ: benzodiazepine binding of <3H>title comp. to BZ binding site of GABAA receptor after
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photoincorporation of chlordiazepoxide studied by saturation assay; incubation for 30 min at 20 deg C; radioactivity measured by scintillation counting Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
4.3
Measurement pX
8.37
McKernan, Ruth M.; Farrar, Sophie; Collins, Ian; Emms, Frances; Asuni, Ayodeji; Quirk, Kathleen; Broughton, Howard; Molecular Pharmacology; vol. 54; nb. 1; (1998); p. 33 - 43, View in Reaxys 503 of 976
504 of 976
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Synaptosome: membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
360 nM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
100
Measurement pX
7.72
Concomitants: Compound RN
754371; 759557
Concomitants: Compound name
[3H]-Diazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
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Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Synaptosome: membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.0360000 nM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
21.7
Measurement pX
1
Concomitants: Compound RN
754371; 759557
Concomitants: Compound name
[3H]-Diazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Synaptosome: membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.360000 nM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
45.9
Measurement pX
9.37
Concomitants: Compound RN
754371; 759557
Concomitants: Compound name
[3H]-Diazepam; Clonazepam
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507 of 976
Concomitants: Compound role
RAD; NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Synaptosome: membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
3.60000 nM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
86.3
Measurement pX
9.24
Concomitants: Compound RN
754371; 759557
Concomitants: Compound name
[3H]-Diazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Synaptosome: membrane
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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509 of 976
Substance Name
Flunitrazepam
Substance Dose
36 nM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
98.8
Measurement pX
8.72
Concomitants: Compound RN
754371; 759557
Concomitants: Compound name
[3H]-Diazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
CD rat
Organs/Tissues
brain
Cells/Cell Lines
cells
Cell Fraction
Synaptosome: membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
0.688
Measurement pX
9.16
Concomitants: Compound RN
754371; 759557
Concomitants: Compound name
[3H]-Diazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
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Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.2
Measurement pX
8.66
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.5
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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512 of 976
Measurement pX
8.6
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
4.5
Measurement pX
8.35
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p28472 + p18507 + p31644
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
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Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.1
Measurement pX
8.68
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-6 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6
Target Uniprot ID
p28472 + p18507 + q16445
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-6 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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515 of 976
Quantitative value
2000
Measurement pX
5.7
Concomitants: Compound RN
754371; 6182319
Concomitants: Compound name
Diazepam; [3H]-Ro 15-4513
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
IC50
Unit
nM
Quantitative value
3
Measurement pX
8.52
Concomitants: Compound RN
702691; 702691
Concomitants: Compound name
Flunitrazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Bioassay Category
Metabolism/Transport
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
plasma
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
fu
Quantitative value
0.21
Votano, Joseph R.; Parham, Marc; Hall, L. Mark; Hall, Lowell H.; Kier, Lemont B.; Oloff, Scott; Tropsha, Alexander; Journal of Medicinal Chemistry; vol. 49; nb. 24; (2006); p. 7169 - 7181, View in Reaxys 516 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor blocking agent Target : Xenopus laevis oocyte, transfectedBioassay : oocyte expressed GABAA receptors composed of α1, α2, α1 subunits; GABA conc. (3-8 μmol/l) corresponded to conc. eliciting 5-10 percent of maximal current amplitude (EC5-10); control: sol. contained GABA without title comp. chamber with oocyte perfused with sol. contained GABA (3-8 μmol/l or 1 mmol/l) and title comp. by fast perfusion system; GABA-induced chloride currents (IGABA) measured by two-electrode voltage-clamp
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
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Qualitative Results
title comp. inhibited IGABA (11 percent at 1 μmol/l); at GABA conc. 1 mmol/l title comp. did not affect IGABA
Measurement Parameter
Qualitative
Khom; Baburin; Timin; Hohaus; Sieghart; Hering; Molecular Pharmacology; vol. 69; nb. 2; (2006); p. 640 - 649, View in Reaxys 517 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor blocking agent Target : Xenopus laevis oocyte, transfectedBioassay : oocyte expressed GABAA receptors composed of α1, α2 subunits; GABA conc. (3-8 μmol/l) corresponded to conc. eliciting 5-10 percent of maximal current amplitude (EC5-10); control: sol. contained GABA without title comp. chamber with oocyte perfused with sol. contained GABA (3-8 μmol/l) and title comp. by fast perfusion system; GABA-induced chloride currents (IGABA) measured by two-electrode voltage-clamp method at holding potential
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
title comp. did not inhibit IGABA statistically significant (9 percent)
Measurement Parameter
Qualitative
Khom; Baburin; Timin; Hohaus; Sieghart; Hering; Molecular Pharmacology; vol. 69; nb. 2; (2006); p. 640 - 649, View in Reaxys 518 of 976
519 of 976
Target Name
UDP-glucuronosyltransferase 2B7*2 [human]
Target Synonyms
udp-glucuronosyltransferase 2b7*2
Target, Subunit, Species
UDP-glucuronosyltransferase 2B7*2 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7*2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
52
Measurement pX
4.28
Metabolite RN
24366853
Metabolite name
4-Hydroxyestrone 4-O-glucuronide
Concomitants: Compound RN
2221832; 78881; 85594; 8188580; 506008
Concomitants: Compound name
4-Hydroxy-Estrone; Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; DMSO
Concomitants: Compound role
SUB; COM; COM; COM; SLV
Target Name
UDP-glucuronosyltransferase 2B7*2 [human]
Target Synonyms
udp-glucuronosyltransferase 2b7*2
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Target, Subunit, Species
UDP-glucuronosyltransferase 2B7*2 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7*2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
61
Measurement pX
4.21
Metabolite RN
24400694
Metabolite name
4-Hydroxyestradiol 4-O-glucuronide
Concomitants: Compound RN
2219366; 78881; 85594; 8188580; 506008
Concomitants: Compound name
4-Hydroxyestradiol; Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; DMSO
Concomitants: Compound role
SUB; COM; COM; COM; SLV
Target Name
UDP-glucuronosyltransferase 2B7*2 [human]
Target Synonyms
udp-glucuronosyltransferase 2b7*2
Target, Subunit, Species
UDP-glucuronosyltransferase 2B7*2 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7*2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
47
Measurement pX
4.33
Metabolite RN
4944536
Metabolite name
Nalorphine glucuronide
Concomitants: Compound RN
78881; 85594; 8188580; 506008; 50597
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522 of 976
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; DMSO; Nalorphine
Concomitants: Compound role
COM; COM; COM; SLV; SUB
Target Name
UDP-glucuronosyltransferase 2B7*2 [human]
Target Synonyms
udp-glucuronosyltransferase 2b7*2
Target, Subunit, Species
UDP-glucuronosyltransferase 2B7*2 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7*2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
93
Measurement pX
4.03
Metabolite RN
10411102
Metabolite name
Buprenorphine 3-O-glucuronide
Concomitants: Compound RN
78881; 85594; 8188580; 6182863; 506008
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; Buprenorphine; DMSO
Concomitants: Compound role
COM; COM; COM; SUB; SLV
Target Name
UDP-glucuronosyltransferase 1-1 [human]
Target Synonyms
bilirubin-specific udpgt isozyme 1; gnt1; hug-br1; udp-glucuronosyltransferase 1-1; udp-glucuronosyltransferase 1-a; udp-glucuronosyltransferase 1a1; udpgt 1-1; ugt-1a; ugt1; ugt1*1; ugt1-01; ugt1.1; ugt1a; ugt1a1
Target Uniprot ID
p22309
Target, Subunit, Species
UDP-glucuronosyltransferase 1-1 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
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524 of 976
Measurement Parameter
Ki
Unit
µM
Quantitative value
206
Measurement pX
3.69
Metabolite RN
10411102
Metabolite name
Buprenorphine 3-O-glucuronide
Concomitants: Compound RN
78881; 85594; 8188580; 6182863; 506008
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; Buprenorphine; DMSO
Concomitants: Compound role
COM; COM; COM; SUB; SLV
Target Name
UDP-glucuronosyltransferase 1-1 [human]
Target Synonyms
bilirubin-specific udpgt isozyme 1; gnt1; hug-br1; udp-glucuronosyltransferase 1-1; udp-glucuronosyltransferase 1-a; udp-glucuronosyltransferase 1a1; udpgt 1-1; ugt-1a; ugt1; ugt1*1; ugt1-01; ugt1.1; ugt1a; ugt1a1
Target Uniprot ID
p22309
Target, Subunit, Species
UDP-glucuronosyltransferase 1-1 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
808
Measurement pX
3.09
Metabolite RN
11305843
Metabolite name
2-Hydroxyestrone 2-O-glucuronide
Concomitants: Compound RN
2221833; 78881; 85594; 8188580; 506008
Concomitants: Compound name
2-Hydroxy-Estrone; Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; DMSO
Concomitants: Compound role
SUB; COM; COM; COM; SLV
Target Name
UDP-glucuronosyltransferase 1-1 [human]
Target Synonyms
bilirubin-specific udpgt isozyme 1; gnt1; hug-br1; udp-glucuronosyltransferase 1-1; udp-glucuronosyltransferase 1-a; udp-glucuronosyltransferase 1a1; udpgt 1-1; ugt-1a; ugt1; ugt1*1; ugt1-01; ugt1.1; ugt1a; ugt1a1
Target Uniprot ID
p22309
Target, Subunit, Species
UDP-glucuronosyltransferase 1-1 [human]
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Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
402
Measurement pX
3.4
Metabolite RN
11305843
Metabolite name
2-Hydroxyestrone 2-O-glucuronide
Concomitants: Compound RN
2219367; 78881; 85594; 8188580; 506008
Concomitants: Compound name
2-Hydroxyestradiol; Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; DMSO
Concomitants: Compound role
SUB; COM; COM; COM; SLV
Target Name
UDP-glucuronosyltransferase 1-3 [human]
Target Synonyms
gnt1; udp-glucuronosyltransferase 1-3; udp-glucuronosyltransferase 1-c; udp-glucuronosyltransferase 1a3; udpgt 1-3; ugt-1c; ugt1; ugt1*3; ugt1-03; ugt1.3; ugt1a3; ugt1c
Target Uniprot ID
p35503
Target, Subunit, Species
UDP-glucuronosyltransferase 1-3 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Homogenate
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Qualitative value
NA
Measurement pX
1
Metabolite RN
10411102
Metabolite name
Buprenorphine 3-O-glucuronide
Concomitants: Compound RN
78881; 85594; 8188580; 6182863; 506008
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527 of 976
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; Buprenorphine; DMSO
Concomitants: Compound role
COM; COM; COM; SUB; SLV
Target Name
UDP-glucuronosyltransferase 1-3 [human]
Target Synonyms
gnt1; udp-glucuronosyltransferase 1-3; udp-glucuronosyltransferase 1-c; udp-glucuronosyltransferase 1a3; udpgt 1-3; ugt-1c; ugt1; ugt1*3; ugt1-03; ugt1.3; ugt1a3; ugt1c
Target Uniprot ID
p35503
Target, Subunit, Species
UDP-glucuronosyltransferase 1-3 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Homogenate
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
22
Measurement pX
4.66
Metabolite RN
11305843
Metabolite name
2-Hydroxyestrone 2-O-glucuronide
Concomitants: Compound RN
2221833; 78881; 85594; 8188580; 506008
Concomitants: Compound name
2-Hydroxy-Estrone; Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; DMSO
Concomitants: Compound role
SUB; COM; COM; COM; SLV
Target Name
UDP-glucuronosyltransferase 1-3 [human]
Target Synonyms
gnt1; udp-glucuronosyltransferase 1-3; udp-glucuronosyltransferase 1-c; udp-glucuronosyltransferase 1a3; udpgt 1-3; ugt-1c; ugt1; ugt1*3; ugt1-03; ugt1.3; ugt1a3; ugt1c
Target Uniprot ID
p35503
Target, Subunit, Species
UDP-glucuronosyltransferase 1-3 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Homogenate
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Quantitative value
30
Measurement pX
4.52
Metabolite RN
11305843
Metabolite name
2-Hydroxyestrone 2-O-glucuronide
Concomitants: Compound RN
2219367; 78881; 85594; 8188580; 506008
Concomitants: Compound name
2-Hydroxyestradiol; Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Phosphatidylcholine; DMSO
Concomitants: Compound role
SUB; COM; COM; COM; SLV
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |drug dependence Bioassay : simple experimental epidemiologic survey of prescription forgeries in community pharmacies (1253 pharmacies) in the Midi-Pyrenees area (southwest France)
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
17 percent of the all forged prescription was made to obtain title compound
Measurement Parameter
Qualitative
Lapeyre-Mestre; Damase-Michel; Adams; Michaud; Montastruc; European Journal of Clinical Pharmacology; vol. 52; nb. 1; (1997); p. 37 - 39, View in Reaxys 529 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : receptor binding affinityTarget : astrocytoma A172 cell membranesBioassay : membrane fragments immobilized on IAM chromatographic phase
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
molecular target: α-aminobutyric acid receptor
Measurement Parameter
Qualitative
Kitabatake; Moaddel; Cole; Gandhari; Frazier; Hartenstein; Rosenberg; Bernier; Wainer; Analytical Chemistry; vol. 80; nb. 22; (2008); p. 8673 - 8680, View in Reaxys 530 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : receptor binding affinityTarget : astrocytoma 1321N1 cell membranes of humanBioassay : membrane fragments immobilized on IAM chromatographic phase
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
molecular target: α-aminobutyric acid receptor
Measurement Parameter
Qualitative
Kitabatake; Moaddel; Cole; Gandhari; Frazier; Hartenstein; Rosenberg; Bernier; Wainer; Analytical Chemistry; vol. 80; nb. 22; (2008); p. 8673 - 8680, View in Reaxys 531 of 976
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
In Vitro (others)
Bioassay Details
Effect : receptor binding affinityTarget : astrocytoma A172 cell membranesBioassay : membrane fragments immobilized on IAM chromatographic phase; dissociation constant (Kd)
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Kd
Unit
nM
Quantitative value
0.81
Measurement pX
9.09
Kitabatake; Moaddel; Cole; Gandhari; Frazier; Hartenstein; Rosenberg; Bernier; Wainer; Analytical Chemistry; vol. 80; nb. 22; (2008); p. 8673 - 8680, View in Reaxys 532 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : receptor binding affinityTarget : astrocytoma 1321N1 cell membranes of humanBioassay : membrane fragments immobilized on IAM chromatographic phase; dissociation constant (Kd)
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Kd
Unit
nM
Quantitative value
1.05
Measurement pX
8.98
Kitabatake; Moaddel; Cole; Gandhari; Frazier; Hartenstein; Rosenberg; Bernier; Wainer; Analytical Chemistry; vol. 80; nb. 22; (2008); p. 8673 - 8680, View in Reaxys 533 of 976
Target Name
Ionotropic gamma aminobutyric acid receptor [Rattus norvegicus]
Target Synonyms
ionotropic gamma aminobutyric acid receptor
Target, Subunit, Species
Ionotropic gamma aminobutyric acid receptor [Rattus norvegicus]
Target Mutant/Chimera Details
Ionotropic gamma aminobutyric acid receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Kd
Unit
nM
Quantitative value
1.3
Deviation
0.200000
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Measurement pX
8.89
Concomitants: Compound RN
759557
Concomitants: Compound name
Clonazepam
Concomitants: Compound role
NSB
Target Name
Ionotropic gamma aminobutyric acid receptor [Rattus norvegicus]
Target Synonyms
ionotropic gamma aminobutyric acid receptor
Target, Subunit, Species
Ionotropic gamma aminobutyric acid receptor [Rattus norvegicus]
Target Mutant/Chimera Details
Ionotropic gamma aminobutyric acid receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]-Flunitrazepam
Measurement Parameter
Bmax
Unit
pmol/mg protein
Quantitative value
0.84
Deviation
0.0500000
Concomitants: Compound RN
759557
Concomitants: Compound name
Clonazepam
Concomitants: Compound role
NSB
Target Name
Gamma hydroxybutyric acid receptor [Rattus norvegicus]
Target Synonyms
gamma hydroxybutyric acid receptor
Target, Subunit, Species
Gamma hydroxybutyric acid receptor [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma hydroxybutyric acid receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
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Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
10 µM
Measurement Parameter
% Inhibition
Qualitative value
NA
Measurement pX
1
Concomitants: Compound RN
24175947; 24175947
Concomitants: Compound name
NCS-382; [3H]-NCS-382
Concomitants: Compound role
NSB; RAD
Target Name
Gamma hydroxybutyric acid receptor [Rattus norvegicus]
Target Synonyms
gamma hydroxybutyric acid receptor
Target, Subunit, Species
Gamma hydroxybutyric acid receptor [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma hydroxybutyric acid receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
10 µM
Measurement Parameter
%
Measurement Object
stimulated binding
Qualitative value
NA
Measurement pX
1
Concomitants: Compound RN
24175947; 24175947
Concomitants: Compound name
NCS-382; [3H]-NCS-382
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-6 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
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Target Synonyms
gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2
Target Uniprot ID
p30191 + p18508 + p63138
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-6 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
10000
Measurement pX
1
Concomitants: Compound RN
6182319
Concomitants: Compound name
[3H]-Ro 15-4513
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-4 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit delta [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-4; gabra4; gamma-aminobutyric acid receptor subunit alpha-4 + gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta
Target Uniprot ID
p28471 + p63079 + p18506
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-4 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit delta [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-4 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit delta [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
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Cells/Cell Lines
HEK293T cell line
Substance RN
702691View in Reaxys
Substance Name
FLUNITRAZEPAM
Measurement Parameter
Ki
Unit
nM
Qualitative value
>>
Quantitative value
1000
Measurement pX
6
Concomitants: Compound RN
6182319
Concomitants: Compound name
[3H]-Ro 15-4513
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : formula Ia metabolite formation; effect onTarget : liver cytosol of humanBioassay : Inhibition Studies with AKR Competitors Inhibition studies were also conducted using some of the AKR competitors listed in Table 2.; Note that AKR1C2/3 inhibitor 5beta-cholanic acid 3alpha, 7-alphadiol, a bile acid displayed IC50s of 0.21 um and 74.4 uM for AKR1C2 and AKR1C3, respectively, using tibolone
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
title compound at 60&micro;M inhibited metabolism of 14C-radiolabelled compound of Formula Ia by 51%
Measurement Parameter
Qualitative
Patent; Ghosal, Anima; Kishnani, Narendra S.; Alton, Kevin B.; White, Ronald E.; US2006/276404; (2006); (A1) English, View in Reaxys 540 of 976
Target Name
UDP-glucuronosyltransferase 2B7 [human]
Target Synonyms
3,4-catechol estrogen-specific udpgt; udp-glucuronosyltransferase 2b7; udp-glucuronosyltransferase 2b9; udpgt 2b7; udpgt 2b9; udpgth-2; ugt2b7; ugtb2b9
Target Uniprot ID
p16662
Target PDB ID
2o6l
Target, Subunit, Species
UDP-glucuronosyltransferase 2B7 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
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542 of 976
Measurement Object
ALL
Quantitative value
25
Measurement pX
4.6
Concomitants: Compound RN
78881; 85594; 93704
Concomitants: Compound name
[14C]-Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Morphine
Concomitants: Compound role
MAR; COM; SUB
Target Name
UDP-glucuronosyltransferase 2B7 [human]
Target Synonyms
3,4-catechol estrogen-specific udpgt; udp-glucuronosyltransferase 2b7; udp-glucuronosyltransferase 2b9; udpgt 2b7; udpgt 2b9; udpgth-2; ugt2b7; ugtb2b9
Target Uniprot ID
p16662
Target PDB ID
2o6l
Target, Subunit, Species
UDP-glucuronosyltransferase 2B7 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
µM
Measurement Object
ALL
Quantitative value
31
Measurement pX
4.51
Concomitants: Compound RN
3595791; 78881; 85594
Concomitants: Compound name
[14C]-Zidovudine; Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone
Concomitants: Compound role
SUB; COM; COM
Target Name
Serum albumin [human]
Target Synonyms
alb; serum albumin
Target Uniprot ID
p02768
Target PDB ID
1ao6; 1bj5; 1bke; 1bm0; 1e78; 1e7a; 1e7b; 1e7c; 1e7e; 1e7f; 1e7g; 1e7h; 1e7i; 1gni; 1gnj; 1h9z; 1ha2; 1hk1; 1hk2; 1hk3; 1hk4; 1hk5; 1n5u; 1o9x; 1tf0; 1uor; 1ysx; 2bx8; 2bxa; 2bxb; 2bxc; 2bxd; 2bxe; 2bxf; 2bxg; 2bxh; 2bxi; 2bxk; 2bxl; 2bxm; 2bxn; 2bxo; 2bxp; 2bxq; 2esg; 2i2z; 2i30; 2n0x; 2vdb; 2vue; 2vuf; 2xsi; 2xvq; 2xvu; 2xvv; 2xvw; 2xw0; 2xw1; 2ydf; 3a73; 3b9l; 3b9m; 3cx9; 3jqz; 3jry; 3lu6; 3lu7; 3lu8; 3sqj; 3tdl; 3uiv; 4bke; 4e99; 4emx; 4g03; 4g04; 4hgk; 4hgm; 4iw1; 4iw2; 4k2c; 4k71; 4l8u; 4l9k; 4l9q; 4la0; 4lb2; 4lb9; 4n0f; 4n0u; 4s1y; 4z69; 5fuo; 5id7; 5ifo; 5ijf; 5ujb; 5x52
Target, Subunit, Species
Serum albumin [human]
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Target Mutant/Chimera Details
Serum albumin [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : human serum albumin; binding ofBioassay : TRANSIL&reg; technology
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
LogHSA -3.046 no_unit
Measurement Parameter
Qualitative
Quantitative value
-3.046
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
inhibitory influence on respiration in anesthetized cats (chloralose/urethane and pentobarbital anesthesia), 0.005-3.325 mg/kg i.v. administration
Measurement Parameter
Qualitative
Danneberg; Arzneimittel-Forschung/Drug Research; vol. 36; nb. 3 A; (1986); p. 610 - 615, View in Reaxys 544 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
in vitro central nervous system receptors binding affinities: <3H>flunitrazepam (benzodiazepine receptor) pIC50= 8.30 in male rat cerebral cortices
Measurement Parameter
Qualitative
Nicholls, Ian A.; Alewood, Paul F.; Brinkworth, Ross I.; Morrison, Stuart F.; Andrews, Peter R.; Journal of Chemical Research, Miniprint; nb. 10; (1993); p. 2811 - 2826, View in Reaxys 545 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
effect of cumulative concentrations on peripheral neuromuscular function (in-vitro rat phrenic nerve-hemidiaphragm preparation)
Measurement Parameter
Qualitative
Driessen; Vree; Booij; et al.; Journal of Pharmacy and Pharmacology; vol. 36; nb. 4; (1984); p. 244 - 247, View in Reaxys 546 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
inhibition of specific 3H-flunitrazepam binding in in vitro preparations of rat cerebral cortex memranes at O deg C; IC50 3.0 nM
Measurement Parameter
Qualitative
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Dodd, Robert H.; Doisy, Xavier; Potier, Pierre; Potier, Marie-Claude; Rossier, Jean; Heterocycles; vol. 28; nb. 2; (1989); p. 1101 - 1113, View in Reaxys 547 of 976
Substance Effect
Psychotropic
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : (3)H-flunitrazepam bindingTarget : Wistar rat synaptosomesBioassay : amount of (3)H-flunitrazepam receptors (Bmax); dissociation constant (Kd) of ligand-receptor complex synaptosomes isol. from brain of male Wistar rats weighing 150-200 g; label. test comp.; in the pres. and abs. of TMD; 22 deg C; radioact. meas. on a Beat-1 liquid. scint. counter
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
Bmax: 30.0-31.5 fM/mg protein in the pres. and abs. of TMD; Kd: 2.27-7.14 pM/mg protein in the pres. and abs. of TMD; IC50 of (3)H-imipramine in the pres. of TMD 0.53 mM
Measurement Parameter
Qualitative
Stradomskii; Klimontova; Ryabukhin; Pharmaceutical Chemistry Journal; vol. 31; nb. 1; (1997); p. 7 - 8, View in Reaxys 548 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |agonist Target : Xenopus laevis oocytes expressing human GABAA receptorBioassay : GABA: α-aminobutyric acid; 5α3α: 5α-pregnan-3α-ol-20-one electrical recordings made from oocytes expressing human α1α1α2L GABAA receptors; oocytes voltage-clamped at -60 mV in two-electrode voltage clamp mode; current induced by propofol, sodium pentobarbitone, 5α3α and trichloroethanol
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.300000 µM
Qualitative Results
title comp. potentiated propofol-, pentobarbitone- and 5α3α-induced inward currents
Measurement Parameter
Qualitative
Pistis, Marco; Belelli, Delia; Peters, John A.; Lambert, Jeremy J.; British Journal of Pharmacology; vol. 122; nb. 8; (1997); p. 1707 - 1719, View in Reaxys 549 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : exposureBioassay : accordance between data sources, reliability 1136 subjects age >65 years: 318 cases (hip fractures), 818 control; 276 men (mean age 79 years), 860 women (81); data sources: patient questionnaire, medical records, plasma samples (HPLC)
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Route of Adm.
oral administration
Qualitative Results
concordance between questionnaires or medical records and plasma samples (table)
Measurement Parameter
Qualitative
Moore; Pierfitte; Pehourcq; Lagnaoui; Begaud; Clinical Pharmacology and Therapeutics; vol. 69; nb. 6; (2001); p. 445 - 450, View in Reaxys 550 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p28472 + p18507 + p14867
Target PDB ID
4cof
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Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Substance RN
702691View in Reaxys
Substance Name
146
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.2
Measurement pX
8.66
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p28472 + p18507 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Substance RN
702691View in Reaxys
Substance Name
146
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.5
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Measurement pX
8.6
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p28472 + p18507 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Substance RN
702691View in Reaxys
Substance Name
146
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
4.5
Measurement pX
8.35
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p28472 + p31644 + p18507
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Substance RN
702691View in Reaxys
Substance Name
146
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.1
Measurement pX
8.68
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-6 [human];+Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p28472 + q16445 + p18507
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-6 [human]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
LTK-
Substance RN
702691View in Reaxys
Substance Name
146
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
2000
Measurement pX
5.7
Concomitants: Compound RN
754371; 6182319
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound name
Diazepam; [3H]-Ro 15-4513
Concomitants: Compound role
NSB; RAD
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
t1/2 abs
Unit
hour
Quantitative value
0.144
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 556 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Tlag
Unit
hour
Quantitative value
0.266
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 557 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Dosing Regimen
Single
Measurement Parameter
t1/2 [lambda1]
Unit
hour
Quantitative value
2.07
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 558 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
13.5
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 559 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Cl/F
Unit
L/h/kg
Quantitative value
0.278
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 560 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
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Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Vd ss/F
Unit
L/kg
Quantitative value
4.44
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 561 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Blood/Serum ratio
Quantitative value
0.75
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 562 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Repeated
Measurement Parameter
t1/2 abs
Unit
hour
Quantitative value
1.47
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 563 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
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Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Repeated
Measurement Parameter
t1/2 [lambda1]
Unit
hour
Quantitative value
1.9
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 564 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Repeated
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
19.2
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 565 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Repeated
Measurement Parameter
Cl/F
Unit
L/h/kg
Quantitative value
0.278
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 566 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
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Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Repeated
Measurement Parameter
Vd/F
Unit
L/kg
Quantitative value
5.63
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 567 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
ClH
Unit
L/h/kg
Quantitative value
0.235
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 568 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
ER
Quantitative value
0.154
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 569 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
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Biological Species/NCBI ID
Human Caucasian
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
Vd ss
Unit
L/kg
Quantitative value
3.76
Boxenbaum; Posmanter; Macasieb; Geitner; Weinfeld; Moore; Darragh; O'Kelly; Weissman; Kaplan; Journal of Pharmacokinetics and Biopharmaceutics; vol. 6; nb. 4; (1978); p. 283 - 293, View in Reaxys 570 of 976
571 of 976
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
forebrain
Substance RN
702691View in Reaxys
Substance Name
Flunizatrepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.8
Measurement pX
8.55
Concomitants: Compound RN
754371; 754371
Concomitants: Compound name
Diazepam; [3H]-Diazepam
Concomitants: Compound role
NSB; RAD
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
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Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Population State
hypertension
Organs/Tissues
brain cortex
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
[3H]Flunitrazepam
Substance Dose
0.350000 nM
Measurement Parameter
kon
Unit
nM-1.min-1
Quantitative value
0.25
Deviation
0.0500000
Concomitants: Compound RN
759557
Concomitants: Compound name
Clonazepam
Concomitants: Compound role
NSB
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Population State
hypertension
Organs/Tissues
brain cortex
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
[3H]Flunitrazepam
Substance Dose
0.350000 nM
Measurement Parameter
koff
Unit
min-1
Quantitative value
0.045
Deviation
0.00200000
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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573 of 976
574 of 976
Concomitants: Compound RN
759557
Concomitants: Compound name
Clonazepam
Concomitants: Compound role
NSB
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Population State
hypertension
Organs/Tissues
brain cortex
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
[3H]Flunitrazepam
Substance Dose
0.350000 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
0.18
Measurement pX
9.74
Concomitants: Compound RN
759557
Concomitants: Compound name
Clonazepam
Concomitants: Compound role
NSB
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
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Biological Species/NCBI ID
575 of 976
576 of 976
human
Population State
hypertension
Organs/Tissues
brain cortex
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
[3H]Flunitrazepam
Substance Dose
0.350000 nM
Measurement Parameter
t1/2 off
Unit
minute
Quantitative value
4.6
Concomitants: Compound RN
759557
Concomitants: Compound name
Clonazepam
Concomitants: Compound role
NSB
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Population State
hypertension
Organs/Tissues
brain cortex
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
1.66
Deviation
0.0100000
Measurement pX
8.78
Concomitants: Compound RN
702691; 759557
Concomitants: Compound name
[3H]-Flunitrazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Population State
hypertension
Organs/Tissues
brain cortex
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
6.2
Deviation
0.200000
Measurement pX
8.21
Concomitants: Compound RN
702691; 759557
Concomitants: Compound name
[3H]-Flunitrazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Population State
hypertension
Organs/Tissues
brain cortex
Cell Fraction
Homogenate/Centrifugation/Pellet
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
nH
Quantitative value
0.89
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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578 of 976
579 of 976
Deviation
0.0300000
Concomitants: Compound RN
702691; 759557
Concomitants: Compound name
[3H]-Flunitrazepam; Clonazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p63079 + p19969 + p18508
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]flunitrazepam
Substance Dose
0 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
1.3
Deviation
0.180000
Measurement pX
8.89
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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581 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
hippocampus
Cells/Cell Lines
nerve cell
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]flunitrazepam
Substance Dose
0 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
1.5
Deviation
0.120000
Measurement pX
8.82
Target Name
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2
Target Uniprot ID
p63079 + p19969 + p18508
Target PDB ID
2pr9
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]flunitrazepam
Substance Dose
0 nM
Measurement Parameter
Bmax
Unit
fmol/mg protein
Quantitative value
190
Deviation
12
Target Name
GABA-A receptor [Rattus norvegicus]
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
hippocampus
Cells/Cell Lines
nerve cell
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]flunitrazepam
Substance Dose
0 nM
Measurement Parameter
Bmax
Unit
fmol/mg protein
Quantitative value
1712
Deviation
156
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cells/Cell Lines
nerve cell
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]flunitrazepam
Substance Dose
0 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
1.5
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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584 of 976
Measurement pX
8.82
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain cortex
Cells/Cell Lines
nerve cell
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
[3H]flunitrazepam
Substance Dose
0 nM
Measurement Parameter
Bmax
Unit
fmol/mg protein
Quantitative value
1160
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
parotid gland
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
1.00000E-10 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
3136
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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586 of 976
Deviation
700
Measurement pX
5.5
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor [Rattus norvegicus]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Rattus norvegicus]
Target Mutant/Chimera Details
GABA-A receptor [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
submandibular gland
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
1.00000E-10 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
3450
Deviation
480
Measurement pX
5.46
Concomitants: Compound RN
4763661; 4763661
Concomitants: Compound name
Flumazenil; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
parotid gland
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Dose
1.00000E-10 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
14.9
Deviation
2.60000
Measurement pX
7.83
Concomitants: Compound RN
4264456; 4264456
Concomitants: Compound name
PK 11195; [3H]-PK 11195
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Wistar rat
Organs/Tissues
submandibular gland
Cells/Cell Lines
cells
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
1.00000E-10 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
40.4
Deviation
4.10000
Measurement pX
7.39
Concomitants: Compound RN
4264456; 4264456
Concomitants: Compound name
PK 11195; [3H]-PK 11195
Concomitants: Compound role
NSB; RAD
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Bioassay Name
Binding
Cells/Cell Lines
LTK-
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
8.9
Deviation
2.54000
Measurement pX
8.05
Concomitants: Compound RN
4763661
Concomitants: Compound name
[3H]-Ro 15-1788
Concomitants: Compound role
RAD
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p08220 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Target Transfection
Transfected
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Cells/Cell Lines
LTK-
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 nM
Measurement Parameter
%
Unit
%
Measurement Object
of potentiation
Quantitative value
184
Deviation
43
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound role
COM
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |pharmacokinetics Bioassay : clearance ranged (CL), volume of distribution at steady-state (Vdss) and free fraction in plasma (fu) were calculated title comp. was infused into right jugular vein of rats (173-200 g) for 1 min; blood samples (100 μl) were collecteed from a small incision in the tail; title comp. conc. in blood were determined by HPLC-UV
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 mg/kg
Substance Route of Adm.
intravenous administration
Qualitative Results
CL 75.1 +/- 4.6 ml/min*kg; Vdss 5.3 +/- 0.6 l/kg; fu 11.1 +/- 0.2 percent (table)
Measurement Parameter
Qualitative
Hoogerkamp; Arends; Bomers; Mandema; Voskuyl; Danhof; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 2; (1996); p. 803 - 812, View in Reaxys 591 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |receptor; binding activity Bioassay : specific binding was assessed by subtracting the nonspecific binding (obtained in the presence of 10 mg/l flumazenil) from the total binding; binding characteristic was determined by applying an in vitro filtration assay receptor binding was determined in triplicate by displacement of <3H>-flumazenil at 37 deg C in a GABA-enriched invironment using various conc. of title comp.
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 mg/kg
Substance Route of Adm.
intravenous administration
Measurement Parameter
Ki
Unit
nM
Quantitative value
29
Measurement pX
7.54
Hoogerkamp; Arends; Bomers; Mandema; Voskuyl; Danhof; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 2; (1996); p. 803 - 812, View in Reaxys 592 of 976
Substance Effect
Anticonvulsant
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Bioassay : TLS: threshold for localized seizure activity; EC250 value was defined as the concentration needed to increase the TLS by 250 μA for the total plasma conc. (EC250) and the free conc. (EC250,u) direct cortical stimulation model; title comp. was infused into right jugular vein for 1 min; anticonvulsant effect was expressed as elevation over the averadge base-line TLS and determined during the 30-min period preceding title comp. administration
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Dose
10 mg/kg
Substance Route of Adm.
intravenous administration
Qualitative Results
title comp. rapidly increased TLS by 300 to 500 μA immediately after administration; the effect slowly declined back to base-line in parallel with the decrease in blood concentration; EC250 = 1.2 +/- 0.3 mg/l; EC250,u = 0.21
Measurement Parameter
Qualitative
Hoogerkamp; Arends; Bomers; Mandema; Voskuyl; Danhof; Journal of Pharmacology and Experimental Therapeutics; vol. 279; nb. 2; (1996); p. 803 - 812, View in Reaxys 593 of 976
Bioassay Category
Metabolism/Transport
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
blood
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Blood/Plasma ratio
Quantitative value
0.78
Brown, Hayley S.; Griffin, Michael; Houston, J. Brian; Drug Metabolism and Disposition; vol. 35; nb. 2; (2007); p. 293 301, View in Reaxys 594 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
effect on human plasma protein binding of digoxin at 1 and 3 μg ml-1 (in-vitro)
Measurement Parameter
Qualitative
Castillo Ferrando; Carrasco Prieto; De La Torre Brasas; Journal of Pharmacy and Pharmacology; vol. 35; nb. 7; (1983); p. 462 - 463, View in Reaxys 595 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Ltk- cells expressing human GABAA α6α3α2 receptorBioassay : affinity measured by competition for <3H>Ro15-1788, incubation at 4 deg C in phosphate buffer (pH=7.4), radioactivity detected by liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Qualitative Results
not active up to a concentration of 1 μM
Measurement Parameter
Qualitative
Huang, Qi; Zhang, Weijiang; Liu, Ruiyan; McKernan, Ruth M.; Cook, James M.; Medicinal Chemistry Research; vol. 6; nb. 6; (1996); p. 384 - 391, View in Reaxys 596 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Ltk- cells expressing human GABAA α1α3α2 receptorBioassay : affinity measured by competition for <3H>Ro15-1788, incubation at 4 deg C in phosphate buffer (pH=7.4), radioactivity detected by liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement Parameter
Ki
Unit
nM
Quantitative value
2.2
Measurement pX
8.66
Huang, Qi; Zhang, Weijiang; Liu, Ruiyan; McKernan, Ruth M.; Cook, James M.; Medicinal Chemistry Research; vol. 6; nb. 6; (1996); p. 384 - 391, View in Reaxys 597 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Ltk- cells expressing human GABAA α2α3α2 receptorBioassay : affinity measured by competition for <3H>Ro15-1788, incubation at 4 deg C in phosphate buffer (pH=7.4), radioactivity detected by liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.5
Measurement pX
8.6
Huang, Qi; Zhang, Weijiang; Liu, Ruiyan; McKernan, Ruth M.; Cook, James M.; Medicinal Chemistry Research; vol. 6; nb. 6; (1996); p. 384 - 391, View in Reaxys 598 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Ltk- cells expressing human GABAA α3α3α2 receptorBioassay : affinity measured by competition for <3H>Ro15-1788, incubation at 4 deg C in phosphate buffer (pH=7.4), radioactivity detected by liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
4.5
Measurement pX
8.35
Huang, Qi; Zhang, Weijiang; Liu, Ruiyan; McKernan, Ruth M.; Cook, James M.; Medicinal Chemistry Research; vol. 6; nb. 6; (1996); p. 384 - 391, View in Reaxys 599 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : Ltk- cells expressing human GABAA α5α3α2 receptorBioassay : affinity measured by competition for <3H>Ro15-1788, incubation at 4 deg C in phosphate buffer (pH=7.4), radioactivity detected by liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.1
Measurement pX
8.68
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Huang, Qi; Zhang, Weijiang; Liu, Ruiyan; McKernan, Ruth M.; Cook, James M.; Medicinal Chemistry Research; vol. 6; nb. 6; (1996); p. 384 - 391, View in Reaxys 600 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
parameters of binding to serum proteins at pH 7.4, obtained from the fluorescence method
Measurement Parameter
Qualitative
Maruyama; Furuie; Hibino; Otagiri; Journal of Pharmaceutical Sciences; vol. 81; nb. 1; (1992); p. 16 - 20, View in Reaxys 601 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human α5α3α2 GABAA receptor expr. in mouse L(tk-) cellsBioassay : membranes prep. from cell monolayers incubated with 0.5 nM <3H>Ro15-1788 in pres. of title comp. at 4 degC, 90 min; filtrated; displacement of radioligand detd. from filter-retained radioactivity by liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.11
Measurement pX
8.68
Hadingham; Wafford; Thompson; Palmer; Whiting; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 3; (1995); p. 301 - 309, View in Reaxys 602 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human α5α3α3 GABAA receptor expr. in mouse L(tk-) cellsBioassay : membranes prep. from cell monolayers incubated with 0.5 nM <3H>Ro15-1788 in pres. of title comp. at 4 degC, 90 min; filtrated; displacement of radioligand detd. from filter-retained radioactivity by liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
11
Measurement pX
7.96
Hadingham; Wafford; Thompson; Palmer; Whiting; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 3; (1995); p. 301 - 309, View in Reaxys 603 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human α1α2α2 GABAA receptor expr. in Xenopus oocytesBioassay : EC50 = conc. of title comp. eliciting half maximal response cells impaled with two 1-3 MOhm electrodes cont. 2M KCl, voltage clamped between -40 - -70 mV; perfused with saline; title comp. added to perfusate 30 sec prior to addition of GABA; current response recorded ca. 30 sec
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
EC50
Unit
nM
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Quantitative value
6.9
Measurement pX
8.16
Hadingham; Wafford; Thompson; Palmer; Whiting; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 3; (1995); p. 301 - 309, View in Reaxys 604 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human α1α2α3 GABAA receptor expr. in Xenopus oocytesBioassay : EC50 = conc. of title comp. eliciting half maximal response cells impaled with two 1-3 MOhm electrodes cont. 2M KCl, voltage clamped between -40 - -70 mV; perfused with saline; title comp. added to perfusate 30 sec prior to addition of GABA; current response recorded ca. 30 sec
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
EC50
Unit
nM
Quantitative value
110.7
Measurement pX
6.95
Hadingham; Wafford; Thompson; Palmer; Whiting; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 3; (1995); p. 301 - 309, View in Reaxys 605 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : binding parameters (KD, nM; Bmax, fmol/mg protein) determined by Scatchard analysis membranes from cerebellum of adult rats incub. in buffer (pH 7.4; 90 min; 4 deg C) with <3H>labeled title comp. +/- 10 μM diazepam; binding determined with liquid scintillation
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
2 nM
Qualitative Results
KD: 2.8; Bmax: 1332
Measurement Parameter
Qualitative
Slany; Zezula; Fuchs; Sieghart; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 2; (1995); p. 99 - 105, View in Reaxys 606 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : binding parameters (KD, nM; Bmax, fmol/mg protein) determined by Scatchard analysis HEK cells, native or transfected with cDNAs for rat GABAA receptor α1α3α2- or α1α2-subunits incub. in buffer (pH 7.4; 90 min; 4 deg C) with <3H>labeled title comp. +/- 10 μM diazepam; binding determined with liquid scintillation
Biological Species/NCBI ID
human
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
2 nM
Qualitative Results
increased binding in transfected cells (fmol/mg protein, native: 20; α1α2: 46, KD: 10.9; Bmax: 240; α1α3α2: 246, KD: 3.53; Bmax: 705)
Measurement Parameter
Qualitative
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Slany; Zezula; Fuchs; Sieghart; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 291; nb. 2; (1995); p. 99 - 105, View in Reaxys 607 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : PA3 cellsBioassay : effect on (GABA)A receptor function; Kd, Bmax, GABA shift cells cultured in Dulbecco's modified Eagle's medium; induction with 1 μM dexamethasone; after 2 d 10 μM title comp. and 50 μM GABA
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
Kd = 5.72 nM, Bmax = 141 percent of control, GABA shift: 1.35
Measurement Parameter
Qualitative
Klein; Mascia; Harkness; Hadingham; Whiting; Harris; Journal of Pharmacology and Experimental Therapeutics; vol. 274; nb. 3; (1995); p. 1484 - 1492, View in Reaxys 608 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : PA3 cellsBioassay : effect on (GABA)A receptor subunit α1, α1 protein cells cultured in Dulbecco's modified Eagle's medium; induction with 1 μM dexamethasone; after 2 d 100 μM title comp. and 50 μM GABA; Western blot analysis
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Qualitative
Qualitative value
NA
Measurement pX
1
Klein; Mascia; Harkness; Hadingham; Whiting; Harris; Journal of Pharmacology and Experimental Therapeutics; vol. 274; nb. 3; (1995); p. 1484 - 1492, View in Reaxys 609 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : PA3 cellsBioassay : effect on (GABA)A receptor subunit mRNAs: α1, α1, α2L cells cultured in Dulbecco's modified Eagle's medium; induction with 1 μM dexamethasone; after 2 d 100 μM title comp. and 50 μM GABA; Northern blot analysis
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Qualitative
Qualitative value
NA
Measurement pX
1
Klein; Mascia; Harkness; Hadingham; Whiting; Harris; Journal of Pharmacology and Experimental Therapeutics; vol. 274; nb. 3; (1995); p. 1484 - 1492, View in Reaxys 610 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : elution front and plateau obtained binding on multiple-receptor stationary phase (MR-SP) studied; MR-SP prepared from homogenized brains and IAM-PC packing material; filled into chromatographic coloumn; mobile phase: Tris*HCl pH 7.4 containing title comp.
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.0500000 nM
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Qualitative Results
dissociation constant on GABAA receptor determined by frontal chromatography: 1.3 nmol/l
Measurement Parameter
Qualitative
Moaddel; Cloix; Ertem; Wainer; Pharmaceutical Research; vol. 19; nb. 1; (2002); p. 104 - 107, View in Reaxys 611 of 976
612 of 976
613 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
brain cortex
Cell Fraction
Cytoplasmic membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.72
Measurement pX
8.57
Concomitants: Compound RN
702691; 702691
Concomitants: Compound name
Flunitrazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
brain cortex
Cell Fraction
Cytoplasmic membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
nH
Quantitative value
1.095
Deviation
0.131000
Concomitants: Compound RN
702691; 702691
Concomitants: Compound name
Flunitrazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
frontal cortex
Cell Fraction
Homogenate
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
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615 of 976
616 of 976
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.2
Deviation
0.500000
Measurement pX
8.66
Concomitants: Compound RN
754371; 754371
Concomitants: Compound name
Diazepam; [3H]-Diazepam
Concomitants: Compound role
NSB; RAD
Substance Effect
Anticonvulsant
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
ED50
Unit
µmol/kg
Quantitative value
0.32
Concomitants: Compound RN
135492
Concomitants: Compound name
Pentetrazole
Concomitants: Compound role
IAV
Substance Effect
Anxiolytic
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
ED50
Unit
µmol/kg
Quantitative value
2.5
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p19969
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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617 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
hippocampus
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
6.51
Deviation
1.23000
Measurement pX
8.19
Concomitants: Compound RN
8080906; 702691
Concomitants: Compound name
[3H]-L-655,708; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p19969
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
hippocampus
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
nH
Quantitative value
0.94
Deviation
0.120000
Concomitants: Compound RN
8080906; 702691
Concomitants: Compound name
[3H]-L-655,708; Flunitrazepam
Concomitants: Compound role
RAD; NSB
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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619 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+GABA-A receptor (Gamma 2S) [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-3; gabra-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p63138 + p20236
Target PDB ID
4tk1; 4tk2; 4tk3; 4tk4; 4u90
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + GABA-A receptor (Gamma 2S) [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.00100000 µM
Measurement Parameter
EC50
Unit
nM
Quantitative value
31
Deviation
16
Measurement pX
7.51
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+GABA-A receptor (Gamma 2S) [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p19969 + p63138
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + GABA-A receptor (Gamma 2S) [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
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Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
0.00100000 µM
Measurement Parameter
EC50
Unit
nM
Quantitative value
2
Deviation
1
Measurement pX
8.7
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+GABA-A receptor (Gamma 2S) [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-3; gabra-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p63138 + p20236
Target PDB ID
4tk1; 4tk2; 4tk3; 4tk4; 4u90
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + GABA-A receptor (Gamma 2S) [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
% Stimulation
Unit
%
Quantitative value
128
Deviation
22
Measurement pX
7.28
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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621 of 976
622 of 976
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+GABA-A receptor (Gamma 2S) [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p19969 + p63138
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + GABA-A receptor (Gamma 2S) [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Transfected
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 nM
Measurement Parameter
% Stimulation
Unit
%
Quantitative value
155
Deviation
32
Measurement pX
8.28
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
free fraction (percent unbound = 22.5) of normal human plasma is concentration independent at conc. of the drug ranging from 10 to 10000 ng ml-1 (dialysis for 20 h at 37 deg C and pH 7.4)
Measurement Parameter
Qualitative
Moschitto; Greenblatt; Journal of Pharmacy and Pharmacology; vol. 35; nb. 3; (1983); p. 179 - 180, View in Reaxys 623 of 976
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Target : human GABAA receptors expressed in mouse L(tk-) fibroblastBioassay : Ki=IC50/(1+<radioligand>/Kd), Kd=mean value for radioligand/receptor α1α3α2S receptors stably expressed in fibroblast cells; binding affinities (as Ki) determined in competition assay with 0.75 nM <3H>Ro 15-1788; liquid scintillation
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.2
Measurement pX
8.28
Hadingham, Karen L.; Garrett, Elizabeth M.; Wafford, Keith A.; Bain, Corinna; Heavens, Robert P.; Sirinathsinghji, Dalip J. S.; Whiting, Paul J.; Molecular Pharmacology; vol. 49; nb. 2; (1996); p. 253 - 259, View in Reaxys 624 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Target : human GABAA receptors expressed in mouse L(tk-) fibroblastBioassay : Ki=IC50/(1+<radioligand>/Kd), Kd=mean value for radioligand/receptor α6α3α2S receptors stably expressed in fibroblast cells; binding affinities (as Ki) determined in competition assay with 5 nM <3H>Ro 15-4513; liquid scintillation
Substance RN
702691View in Reaxys
Substance Name
31870
Measurement Parameter
Ki
Unit
nM
Quantitative value
1000
Measurement pX
6
Hadingham, Karen L.; Garrett, Elizabeth M.; Wafford, Keith A.; Bain, Corinna; Heavens, Robert P.; Sirinathsinghji, Dalip J. S.; Whiting, Paul J.; Molecular Pharmacology; vol. 49; nb. 2; (1996); p. 253 - 259, View in Reaxys 625 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : drug interactionTarget : human GABAA receptors expressed in Xenopus oocytesBioassay : oocytes placed in bath with modified Barth's medium and impaled with two 1-3 Mα containing 2M KCl; voltage-clamp; title comp. preapplied 30 s before GABA; concetration-response curves α6α2α2S and α1α2α2S receptors (α6 and α1); effect of title comp. on α-aminobutyric acid (GABA) currents in oocytes
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
title comp. potentiated effect of GABA in α1; no effect in α6
Measurement Parameter
Qualitative
Hadingham, Karen L.; Garrett, Elizabeth M.; Wafford, Keith A.; Bain, Corinna; Heavens, Robert P.; Sirinathsinghji, Dalip J. S.; Whiting, Paul J.; Molecular Pharmacology; vol. 49; nb. 2; (1996); p. 253 - 259, View in Reaxys 626 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human GABAA receptors in human embryonic kidney 293 cellsBioassay : cells transiently transfected with α6α1α2S receptors; affinities of title comp. determined by inhibition of <3H>Ro 15-4513 binding in membranes
Substance RN
702691View in Reaxys
Substance Name
31870
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Substance Dose
1.00000E-07 M
Qualitative Results
had a biphasic curve with low (IC50=380 nM) and high affinity (IC50=14 nM)
Measurement Parameter
Qualitative
Hadingham, Karen L.; Garrett, Elizabeth M.; Wafford, Keith A.; Bain, Corinna; Heavens, Robert P.; Sirinathsinghji, Dalip J. S.; Whiting, Paul J.; Molecular Pharmacology; vol. 49; nb. 2; (1996); p. 253 - 259, View in Reaxys 627 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human GABAA receptors in human embryonic kidney 293 cellsBioassay : cells transiently transfected with α1α1α2S receptors; affinities of title comp. determined by inhibition of <3H>Ro 15-4513 binding in membranes
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-10 M
Measurement Parameter
IC50
Unit
nM
Quantitative value
14
Measurement pX
7.85
Hadingham, Karen L.; Garrett, Elizabeth M.; Wafford, Keith A.; Bain, Corinna; Heavens, Robert P.; Sirinathsinghji, Dalip J. S.; Whiting, Paul J.; Molecular Pharmacology; vol. 49; nb. 2; (1996); p. 253 - 259, View in Reaxys 628 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human GABAA receptors in human embryonic kidney 293 cellsBioassay : cells transiently transfected with α6α1α2S receptors; affinities of title comp. determined by inhibition of <3H>Ro 15-4513 binding in membranes
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-10 M
Measurement Parameter
IC50
Unit
nM
Quantitative value
210
Measurement pX
6.68
Hadingham, Karen L.; Garrett, Elizabeth M.; Wafford, Keith A.; Bain, Corinna; Heavens, Robert P.; Sirinathsinghji, Dalip J. S.; Whiting, Paul J.; Molecular Pharmacology; vol. 49; nb. 2; (1996); p. 253 - 259, View in Reaxys 629 of 976
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p18505 + p14867
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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630 of 976
631 of 976
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
11.5
Deviation
1.60000
Measurement pX
7.94
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p18505 + p47869
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.2
Deviation
0.400000
Measurement pX
8.28
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
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Target Synonyms
gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s) + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p18505 + p34903
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
15.7
Deviation
3.60000
Measurement pX
7.8
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
Gamma-aminobutyric acid receptor subunit alpha-5 [human];+Gamma-aminobutyric acid receptor subunit beta-1 [human];+GABA-A receptor (Gamma 2S) [human]
Target Synonyms
gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5 + gaba(a) receptor subunit beta-1; gabrb1; gamma-aminobutyric acid receptor subunit beta-1 + gaba-a receptor (gamma 2s)
Target Uniprot ID
p31644 + p18505
Target, Subunit, Species
GABA-A receptor (Gamma 2S) [human]; Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-1 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-1 [human]:Wild + GABA-A receptor (Gamma 2S) [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Cells/Cell Lines
HEK293 cell line
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
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Unit
nM
Quantitative value
5.5
Deviation
0.400000
Measurement pX
8.26
Concomitants: Compound RN
702691; 4763661
Concomitants: Compound name
Flunitrazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : neural network activity; reduction ofTarget : hippocampal neuron culture of Sprague-Dawley ratBioassay : 00235] Example 6 : Mg++ and GABAB Agonists Increase Synaptic Plasticiy on a Long Term Basis ; Various Other Agents that Reduce Ca++ Flux Increase Synaptic Plasticity on a Short Term Basis. [00236] We sought to assess the ability of various treatments to increase synaptic plasticity. In functional neural
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
pre-treatment with title compound at 5 &micro;mol/l for 48 hours inhibited global neuronal network activity; but no effect on synaptic plasticity was observed (figures are given)
Measurement Parameter
Qualitative
Patent; MASSACHUSETTS INSTITUTE OF TECHNOLOGY; WO2005/37215; (2005); (A2) English, View in Reaxys 634 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : plasticity of presynaptic terminals; enhancing ofTarget : hippocampal neuron culture of Sprague-Dawley ratBioassay : 00235] Example 6 : Mg++ and GABAB Agonists Increase Synaptic Plasticiy on a Long Term Basis ; Various Other Agents that Reduce Ca++ Flux Increase Synaptic Plasticity on a Short Term Basis. [00236] We sought to assess the ability of various treatments to increase synaptic plasticity. In functional neural
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
pre-treatment with title compound at 5 &micro;mol/l for 4-6 hours led to enhancing of inhibitory synaptic transmission and corresponding reduction in overall activity of neural network, resulting in formation of a highly plastic network (increase in
Measurement Parameter
Qualitative
Patent; MASSACHUSETTS INSTITUTE OF TECHNOLOGY; WO2005/37215; (2005); (A2) English, View in Reaxys 635 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
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Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.2
Deviation
0.0100000
Metabolite RN
1226636; 568966; 576896; 1169840; 688458; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
1.29
Deviation
0.0500000
Metabolite RN
1226636; 568966; 576896; 1169840; 688458; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
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Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
S50
Unit
mM
Quantitative value
694
Deviation
104
Metabolite RN
568966; 1169840; 688458; 713494; 751823; 759300
Metabolite name
alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
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Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
S50
Unit
mM
Quantitative value
224
Deviation
44
Metabolite RN
568966; 1169840; 688458; 713494; 751823; 759300
Metabolite name
alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clmax
Unit
mL/min/nmol target
Quantitative value
0.9
Metabolite RN
568966; 1169840; 688458; 713494; 751823; 759300
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Metabolite name
alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clmax
Unit
mL/min/nmol target
Quantitative value
5.7
Metabolite RN
568966; 1169840; 688458; 713494; 751823; 759300
Metabolite name
alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
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Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Alpha
Quantitative value
0.03
Deviation
0.0100000
Metabolite RN
568966; 1169840; 688458; 713494; 751823; 759300
Metabolite name
alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Alpha
Quantitative value
0.36
Deviation
0.190000
Metabolite RN
568966; 1169840; 688458; 713494; 751823; 759300
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Metabolite name
alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.14
Deviation
0.0100000
Metabolite RN
1226636; 568966; 576896; 1169840; 688458; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
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Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
2.38
Deviation
0.0500000
Metabolite RN
1226636; 568966; 576896; 1169840; 688458; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
S50
Unit
mM
Quantitative value
337
Deviation
32
Metabolite RN
1226636; 568966; 1169840; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A5 [human]
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Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clmax
Unit
mL/min/nmol target
Quantitative value
13.4
Metabolite RN
1226636; 568966; 1169840; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Km
Unit
mM
Quantitative value
128
Deviation
21
Metabolite RN
576896; 688458
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Metabolite name
alpha-Hydroxytriazolam; Desmethylflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clint
Unit
mL/min/nmol target
Quantitative value
1.09
Metabolite RN
576896; 688458
Metabolite name
alpha-Hydroxytriazolam; Desmethylflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
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Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Alpha
Quantitative value
0.07
Deviation
0.0200000
Metabolite RN
568966; 1169840; 713494; 751823; 759300
Metabolite name
alpha-Hydroxyalprazolam; 4-Hydroxytriazolam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.064
Deviation
0.00300000
Metabolite RN
688458; 713494; 751823; 759300
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
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Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
pmol/min/pmol target
Quantitative value
0.5
Deviation
0.0700000
Metabolite RN
688458; 713494; 751823; 759300
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clint
Unit
mL/min/nmol target
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Quantitative value
0.72
Metabolite RN
688458; 713494; 751823; 759300
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clint
Unit
mL/min/nmol target
Quantitative value
1.7
Metabolite RN
688458; 713494; 751823; 759300
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
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Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Beta
Quantitative value
0.28
Deviation
0.100000
Metabolite RN
688458; 751823; 759300
Metabolite name
Desmethylflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
Cytochrome P450 2C19 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cyp2c19; cypiic17; cypiic19; cytochrome p450 2c19; cytochrome p450-11a; cytochrome p450-254c; mephenytoin 4-hydroxylase
Target Uniprot ID
p33261
Target PDB ID
4gqs
Target, Subunit, Species
Cytochrome P450 2C19 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C19 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Cells/Cell Lines
BTI-Tn-5B1-4 cell line
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Km
Unit
mM
Quantitative value
89
Deviation
13
Measurement pX
1.05
Metabolite RN
713494
Metabolite name
3-Hydroxyflunitrazepam
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Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p0c2w5 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Target Transfection
Transfected
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Mus musculus
Cells/Cell Lines
fibroblast
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
EC50
Unit
nM
Quantitative value
23.1
Deviation
9.10000
Measurement pX
7.64
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
GABA-A receptor (Gamma 2L) [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gaba-a receptor (gamma 2l) + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p0c2w5 + p08219
Target, Subunit, Species
GABA-A receptor (Gamma 2L) [taurine cattle]; Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor (Gamma 2L) [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
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Target Species (Bioactivity)
taurine cattle
Target Transfection
Transfected
Substance Action on Target
Allosteric Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Electrophysiology
Biological Species/NCBI ID
Mus musculus
Cells/Cell Lines
fibroblast
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Emax(%)
Unit
%
Measurement Object
maximum of GABA reponse
Quantitative value
208
Deviation
27
Concomitants: Compound RN
906818
Concomitants: Compound name
GABA
Concomitants: Compound role
AGO
Target Name
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Synonyms
gnt1; udp-glucuronosyltransferase 1-3; udp-glucuronosyltransferase 1-c; udp-glucuronosyltransferase 1a3; udpgt 1-3; ugt-1c; ugt1; ugt1*3; ugt1-03; ugt1.3; ugt1a3; ugt1c + hlug4; udp-glucuronosyltransferase 2b15; udp-glucuronosyltransferase 2b8; udpgt 2b15; udpgt 2b8; udpgth-3; ugt2b15; ugt2b8 + bilirubin-specific udpgt isozyme 1; gnt1; hug-br1; udp-glucuronosyltransferase 1-1; udpglucuronosyltransferase 1-a; udp-glucuronosyltransferase 1a1; udpgt 1-1; ugt-1a; ugt1; ugt1*1; ugt1-01; ugt1.1; ugt1a; ugt1a1
Target Uniprot ID
p35503 + p54855 + p22309
Target, Subunit, Species
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-3 [human]:Wild; UDP-glucuronosyltransferase 2B15 [human]:Wild; UDP-glucuronosyltransferase 1-1 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
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Unit
%
Quantitative value
62.6
Measurement pX
4.22
Metabolite RN
7962987
Metabolite name
SCH 60663
Concomitants: Compound RN
78881; 85594; 24143771; 1098229; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; Methanol; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SLV; SUB
Target Name
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Synonyms
gnt1; udp-glucuronosyltransferase 1-3; udp-glucuronosyltransferase 1-c; udp-glucuronosyltransferase 1a3; udpgt 1-3; ugt-1c; ugt1; ugt1*3; ugt1-03; ugt1.3; ugt1a3; ugt1c + hlug4; udp-glucuronosyltransferase 2b15; udp-glucuronosyltransferase 2b8; udpgt 2b15; udpgt 2b8; udpgth-3; ugt2b15; ugt2b8 + bilirubin-specific udpgt isozyme 1; gnt1; hug-br1; udp-glucuronosyltransferase 1-1; udpglucuronosyltransferase 1-a; udp-glucuronosyltransferase 1a1; udpgt 1-1; ugt-1a; ugt1; ugt1*1; ugt1-01; ugt1.1; ugt1a; ugt1a1
Target Uniprot ID
p35503 + p54855 + p22309
Target, Subunit, Species
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-3 [human]:Wild; UDP-glucuronosyltransferase 2B15 [human]:Wild; UDP-glucuronosyltransferase 1-1 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
200 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
86.1
Measurement pX
4.49
Metabolite RN
7962987
Metabolite name
SCH 60663
Concomitants: Compound RN
78881; 85594; 24143771; 1098229; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; Methanol; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SLV; SUB
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Target Name
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Synonyms
gnt1; udp-glucuronosyltransferase 1-3; udp-glucuronosyltransferase 1-c; udp-glucuronosyltransferase 1a3; udpgt 1-3; ugt-1c; ugt1; ugt1*3; ugt1-03; ugt1.3; ugt1a3; ugt1c + hlug4; udp-glucuronosyltransferase 2b15; udp-glucuronosyltransferase 2b8; udpgt 2b15; udpgt 2b8; udpgth-3; ugt2b15; ugt2b8 + bilirubin-specific udpgt isozyme 1; gnt1; hug-br1; udp-glucuronosyltransferase 1-1; udpglucuronosyltransferase 1-a; udp-glucuronosyltransferase 1a1; udpgt 1-1; ugt-1a; ugt1; ugt1*1; ugt1-01; ugt1.1; ugt1a; ugt1a1
Target Uniprot ID
p35503 + p54855 + p22309
Target, Subunit, Species
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-3 [human]:Wild; UDP-glucuronosyltransferase 2B15 [human]:Wild; UDP-glucuronosyltransferase 1-1 [human]:Wild
Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
jejunum
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
35.7
Measurement pX
3.74
Metabolite RN
7962987
Metabolite name
SCH 60663
Concomitants: Compound RN
78881; 85594; 24143771; 1098229; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; Methanol; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SLV; SUB
Target Name
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Synonyms
gnt1; udp-glucuronosyltransferase 1-3; udp-glucuronosyltransferase 1-c; udp-glucuronosyltransferase 1a3; udpgt 1-3; ugt-1c; ugt1; ugt1*3; ugt1-03; ugt1.3; ugt1a3; ugt1c + hlug4; udp-glucuronosyltransferase 2b15; udp-glucuronosyltransferase 2b8; udpgt 2b15; udpgt 2b8; udpgth-3; ugt2b15; ugt2b8 + bilirubin-specific udpgt isozyme 1; gnt1; hug-br1; udp-glucuronosyltransferase 1-1; udpglucuronosyltransferase 1-a; udp-glucuronosyltransferase 1a1; udpgt 1-1; ugt-1a; ugt1; ugt1*1; ugt1-01; ugt1.1; ugt1a; ugt1a1
Target Uniprot ID
p35503 + p54855 + p22309
Target, Subunit, Species
UDP-glucuronosyltransferase 1-3 [human]; UDP-glucuronosyltransferase 2B15 [human]; UDPglucuronosyltransferase 1-1 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-3 [human]:Wild; UDP-glucuronosyltransferase 2B15 [human]:Wild; UDP-glucuronosyltransferase 1-1 [human]:Wild
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Target Species (Bioactivity)
human; human; human
Target Transfection
Non Transfected; Non Transfected; Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
jejunum
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
200 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
52.6
Measurement pX
3.74
Metabolite RN
7962987
Metabolite name
SCH 60663
Concomitants: Compound RN
78881; 85594; 24143771; 1098229; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; Methanol; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SLV; SUB
Target Name
UDP-glucuronosyltransferase 2B7 [human]
Target Synonyms
3,4-catechol estrogen-specific udpgt; udp-glucuronosyltransferase 2b7; udp-glucuronosyltransferase 2b9; udpgt 2b7; udpgt 2b9; udpgth-2; ugt2b7; ugtb2b9
Target Uniprot ID
p16662
Target PDB ID
2o6l
Target, Subunit, Species
UDP-glucuronosyltransferase 2B7 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
jejunum
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
Unit
%
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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664 of 976
Quantitative value
21.3
Measurement pX
1
Metabolite RN
24459560
Metabolite name
SCH 488128
Concomitants: Compound RN
78881; 85594; 24143771; 1098229; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; Methanol; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SLV; SUB
Target Name
UDP-glucuronosyltransferase 2B7 [human]
Target Synonyms
3,4-catechol estrogen-specific udpgt; udp-glucuronosyltransferase 2b7; udp-glucuronosyltransferase 2b9; udpgt 2b7; udpgt 2b9; udpgth-2; ugt2b7; ugtb2b9
Target Uniprot ID
p16662
Target PDB ID
2o6l
Target, Subunit, Species
UDP-glucuronosyltransferase 2B7 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
jejunum
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
200 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
44.2
Measurement pX
3.6
Metabolite RN
24459560
Metabolite name
SCH 488128
Concomitants: Compound RN
78881; 85594; 24143771; 1098229; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; Methanol; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SLV; SUB
Target Name
UDP-glucuronosyltransferase 2B7 [human]
Target Synonyms
3,4-catechol estrogen-specific udpgt; udp-glucuronosyltransferase 2b7; udp-glucuronosyltransferase 2b9; udpgt 2b7; udpgt 2b9; udpgth-2; ugt2b7; ugtb2b9
Target Uniprot ID
p16662
Target PDB ID
2o6l
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target, Subunit, Species
UDP-glucuronosyltransferase 2B7 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B7 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
cells
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
200 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
82.8
Measurement pX
4.38
Metabolite RN
24459560
Metabolite name
SCH 488128
Concomitants: Compound RN
78881; 85594; 24143771; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SUB
Target Name
UDP-glucuronosyltransferase 2B15 [human]
Target Synonyms
hlug4; udp-glucuronosyltransferase 2b15; udp-glucuronosyltransferase 2b8; udpgt 2b15; udpgt 2b8; udpgth-3; ugt2b15; ugt2b8
Target Uniprot ID
p54855
Target, Subunit, Species
UDP-glucuronosyltransferase 2B15 [human]
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 2B15 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
cells
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
200 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
96.9
Measurement pX
4.98
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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667 of 976
Metabolite RN
7962987
Metabolite name
SCH 60663
Concomitants: Compound RN
78881; 85594; 24143771; 7981967
Concomitants: Compound name
Uridine 5'-diphosphoglucuronic acid; Saccharo-1,4-lactone; Alamethicin; [14C]-Ezetimibe
Concomitants: Compound role
COM; COM; COM; SUB
Target Name
Aldehyde oxidase [human]
Target Synonyms
aldehyde oxidase 1; aldehyde oxidase; ao; aox1; azaheterocycle hydroxylase
Target Uniprot ID
q06278
Target PDB ID
4uhw; 4uhx; 5epg
Target, Subunit, Species
Aldehyde oxidase [human]
Target Mutant/Chimera Details
Aldehyde oxidase [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Cytosol
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
50 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
49
Measurement pX
4.28
Metabolite RN
3654
Metabolite name
1-Phthalazinone
Concomitants: Compound RN
109240; 506008; 1716295
Concomitants: Compound name
Phthlazine; DMSO; EDTA
Concomitants: Compound role
SUB; SLV; COM
Substance Effect
Behavioural Symptoms
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : discriminative stimulus effectsBioassay : DR: drug responding ( percent responding on flumazenil lever); control response rate represents average of five saline sessions immediately preceding a test; flumazenil 0.32 mg/kg for three monkeys or 0.1 mg/kg for two monkeys diazepam-treated flumazenil discriminating monkeys (training cycles) injected with title comp. during first minute of first test cycle followed by cumulative doses of flumazenil during subsequent cycles; percent DR and response rate ( percent of control) calculated
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Biological Species/NCBI ID
rhesus monkey
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.320000 mg/kg
Substance Route of Adm.
subcutaneous administration
Qualitative Results
title comp. occasioned primarily vehicle-lever responding and attenuated the flumazenil discriminative stimulus, shifted flumazenil dose-effect curve to right, and did not substantially modify response rate
Measurement Parameter
Qualitative
McMahon, Lance R.; France, Charles P.; Journal of Pharmacology and Experimental Therapeutics; vol. 304; nb. 1; (2003); p. 109 - 120, View in Reaxys 668 of 976
Substance Effect
Behavioural Symptoms
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : discriminative stimulus effectsBioassay : DR: drug responding ( percent responding on midazolam lever); control response rate represents average of five saline sessions immediately preceding a test; substitution for midazolam was defined as >= 80 percent responding on midazolamappropriate lever midazolam discriminating monkeys (midazolam training cycles) injected with title comp. during first minute of first test cycle followed by saline or sham injection on subsequent cycles; percent DR and response rate ( percent of control) calculated
Biological Species/NCBI ID
rhesus monkey
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.0100000 mg/kg
Substance Route of Adm.
subcutaneous administration
Qualitative Results
single dose of title comp. (0.032 and 0.1 mg/kg) increased midazolam-lever responding across cycles in a time-related manner (substitution for midazolam was observed within 30 min) and did not substantially modify response rate
Measurement Parameter
Qualitative
McMahon, Lance R.; France, Charles P.; Journal of Pharmacology and Experimental Therapeutics; vol. 304; nb. 1; (2003); p. 109 - 120, View in Reaxys 669 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology stimulation
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Biological Species/NCBI ID
670 of 976
671 of 976
Escherichia coli Topp3
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 µM
Measurement Parameter
Fold-increase
Quantitative value
1.41
Metabolite RN
210932
Metabolite name
7-Hydroxy-4-(trifluoromethyl)coumarin
Concomitants: Compound RN
8706346; 77911
Concomitants: Compound name
7-Benzyloxy-4-trifluoromethylcoumarin; NADPH
Concomitants: Compound role
SUB; COE
Target Name
Cytochrome P450 3A4 [chimpanzee]
Target Synonyms
cytochrome p450 3a4
Target, Subunit, Species
Cytochrome P450 3A4 [chimpanzee]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [chimpanzee]:Wild
Target Species (Bioactivity)
chimpanzee
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology stimulation
Biological Species/NCBI ID
Escherichia coli Topp3
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
100 µM
Measurement Parameter
Fold-increase
Quantitative value
1.55
Metabolite RN
210932
Metabolite name
7-Hydroxy-4-(trifluoromethyl)coumarin
Concomitants: Compound RN
8706346; 77911
Concomitants: Compound name
7-Benzyloxy-4-trifluoromethylcoumarin; NADPH
Concomitants: Compound role
SUB; COE
Target Name
GABA-A receptor [taurine cattle]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.17
Deviation
0.200000
Measurement pX
8.29
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Concomitants: Compound role
NSB; RAD
Target Name
ionotropic gamma aminobutyric acid a receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
ionotropic gamma aminobutyric acid a receptor [human]
Target Mutant/Chimera Details
ionotropic gamma aminobutyric acid a receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
6.5
Deviation
0.500000
Measurement pX
8.19
Concomitants: Compound RN
754371; 4763661
Concomitants: Compound name
Diazepam; [3H]-Ro 15-1788
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Concomitants: Compound role
NSB; RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
no effect on the response to GABA (field-stimulated guinea-pig vas deferens preparation; dose: 1E-5 M)
Measurement Parameter
Qualitative
Doble; Turnbull; Journal of Pharmacy and Pharmacology; vol. 33; nb. 4; (1981); p. 267 - 268, View in Reaxys 674 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : drug interactionBioassay : determination of I/Ic ( GABA-induced peak current in the presence of the drug/ control GABA-induced peak current); I/Imax (Imax: maximal current) in vitro; cells from 6-d-old Wistar rats; electrophysiological recording α-aminobutyric acid (GABA)-induced membrane currents; add. of α-aminobutyric acid (GABA, 3 μM) and bicuculline (BIC,100 μM)
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
title comp. potentiated GABA-induced currents
Measurement Parameter
Qualitative
Feigenspan; Bormann; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 288; nb. 1; (1994); p. 97 104, View in Reaxys 675 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : different brain regions of Harlan Sprague-Dawley ratBioassay : region sepcific changes in benzodiazepine binding site labeled by <3H>-title compound in discrete brain regions of rats made tolerant and dependent upon pentobarbital the brain sections of rats (rendered tolerant by intracerebroventricular infusion with pentobarbital (300 μg/10μl/h for 6 d)) incubated with 1 nM <3H>-labeled title compound for 90 min at 4 deg C, pH 7.1; autoradiograms generated
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
in tolerant rats: significant increase in <3H>-title compound binding sites in frontal cortex and olfactory bulb; in dependent rats: increase in frontal cortex, caudate-putamen, olfactory tubercle, olfactory bulb, globus pallidus and ventral pallidum
Measurement Parameter
Qualitative
Suzuki; Ito; Wellman; Ho Ing Kang; Life Sciences; vol. 57; nb. 5; (1995); p. 63 - 69, View in Reaxys 676 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |pharmacokinetics Bioassay : drugs were administered into femoral vein, blood samples taken from the carotid artery of the womb; pharmacokinetic parameters of the transplacental passage were determined
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
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Substance Dose
5 mg/kg
Qualitative Results
half-life of elimination phase: t1/2α2=83.49 min; foetomaternal conc ratio at equilibrium: FMCReq=0.324; time to reach equilibrium: teq=37.67 min
Measurement Parameter
Qualitative
Staud; Fendrich; Kopecka; Palicka; Laznicek; Pharmaceutical Sciences; vol. 2; nb. 4; (1996); p. 195 - 198, View in Reaxys 677 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : Wistar rats, membranes prepared from the cerebral cortex, cerebellum and spinal cord, enriched with cholesterol, incubated with phosphatidylcholine liposomes, experiments were performed to determine the binding effects in the presence of pregnanolone
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
the potency of pregnanolone was reduced by a factor of 3.2 in cerebral cortex while increased by a factor of 8.4 in spinal cord by title comp. following cholesterol enrichment, little overall change in cerebellum in pregnanolon potency by title comp.
Measurement Parameter
Qualitative
Bennett; Simmonds; British Journal of Pharmacology; vol. 117; nb. 1; (1996); p. 87 - 92, View in Reaxys 678 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : Wistar rats, membranes prepared from the whole brain, enriched with cholesterol, incubated with phosphatidylcholine liposomes, experiments were performed to determine the binding effects in the presence of pregnanolone, propofol, muscimol
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
cholesterol enrichment did not affected the binding of title comp., however, the enhancement of binding by pregnanolone was affected; that of by propofol was reduced in cholesterol-enriched membranes, muscimol was a potentiator of title comp. binding
Measurement Parameter
Qualitative
Bennett; Simmonds; British Journal of Pharmacology; vol. 117; nb. 1; (1996); p. 87 - 92, View in Reaxys 679 of 976
Target Name
Cholecystokinin receptor type A [Rattus norvegicus]
Target Synonyms
cck-a receptor; cck-ar; cck1-r; cckar; cholecystokinin receptor type a; cholecystokinin-1 receptor
Target Uniprot ID
p30551
Target, Subunit, Species
Cholecystokinin receptor type A [Rattus norvegicus]
Target Mutant/Chimera Details
Cholecystokinin receptor type A [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
pancreas
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680 of 976
681 of 976
Substance RN
702691View in Reaxys
Substance Name
flunirazepam
Measurement Parameter
IC50
Unit
µM
Qualitative value
>
Quantitative value
100
Measurement pX
1
Concomitants: Compound RN
1719757
Concomitants: Compound name
Dithiothreitol
Concomitants: Compound role
COM
Target Name
Gastrin/cholecystokinin type B receptor [guinea pig]
Target Synonyms
gastrin/cholecystokinin type b receptor
Target, Subunit, Species
Gastrin/cholecystokinin type B receptor [guinea pig]
Target Mutant/Chimera Details
Gastrin/cholecystokinin type B receptor [guinea pig]:Wild
Target Species (Bioactivity)
guinea pig
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
guinea pig
Organs/Tissues
brain cortex
Substance RN
702691View in Reaxys
Substance Name
flunirazepam
Measurement Parameter
IC50
Unit
µM
Qualitative value
>
Quantitative value
100
Measurement pX
1
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
brain
Substance RN
702691View in Reaxys
Substance Name
flunirazepam
Measurement Parameter
IC50
Unit
µM
Quantitative value
0.0017
Measurement pX
8.77
Concomitants: Compound RN
754371
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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682 of 976
Concomitants: Compound name
[3H]-Diazepam
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Organs/Tissues
brain
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
2.4
Measurement pX
8.62
Concomitants: Compound RN
702691; 702691
Concomitants: Compound name
Flunitrazepam; [3H]-Flunitrazepam
Concomitants: Compound role
NSB; RAD
Dorow; Seidler; Schneider; British Journal of Clinical Pharmacology; vol. 13; nb. 4; (1982); p. 561 - 565, View in Reaxys 683 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Substance Dosing Regimen
Single
Measurement Parameter
kel
Unit
h-1
Quantitative value
0.031
Deviation
0.00800000
Dorow; Seidler; Schneider; British Journal of Clinical Pharmacology; vol. 13; nb. 4; (1982); p. 561 - 565, View in Reaxys 684 of 976
Bioassay Category
Pharmacokinetic
Bioassay Name
Pharmacokinetic
Biological Species/NCBI ID
human
Population State
Healthy
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
2 mg
Substance Route of Adm.
oral administration
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
374/493
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Substance Dosing Regimen
Single
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
23
Dorow; Seidler; Schneider; British Journal of Clinical Pharmacology; vol. 13; nb. 4; (1982); p. 561 - 565, View in Reaxys 685 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Quantitative value
0.4
Deviation
0.0300000
Metabolite RN
1226636; 568966; 688458; 713494
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 686 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
375/493
2018-04-14 18:48:42
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Quantitative value
7.1
Deviation
0.300000
Metabolite RN
1226636; 568966; 688458; 713494
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 687 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
376/493
2018-04-14 18:48:42
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
300
Deviation
24
Metabolite RN
1226636; 568966; 688458; 713494
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 688 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
S50
Unit
µM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
377/493
2018-04-14 18:48:42
Quantitative value
174
Deviation
29
Metabolite RN
1226636; 568966; 688458; 713494
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 689 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clmax
Unit
µL/min/mg protein
Quantitative value
1.4
Metabolite RN
1226636; 568966; 688458; 713494
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
378/493
2018-04-14 18:48:42
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 690 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clmax
Unit
µL/min/mg protein
Quantitative value
12
Metabolite RN
1226636; 568966; 688458; 713494
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 691 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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2018-04-14 18:48:42
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clmax
Unit
µL/min/pmol target
Quantitative value
0.08
Metabolite RN
1226636; 568966; 713494
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 692 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
380/493
2018-04-14 18:48:42
Measurement Parameter
Clmax
Unit
µL/min/nmol target
Quantitative value
0.1
Metabolite RN
688458
Metabolite name
Desmethylflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 693 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Quantitative value
0.007
Deviation
0.00100000
Metabolite RN
24193057; 576896; 1169840; 497190; 688458; 713494
Metabolite name
(3S)-Hydroxyquinine; alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Oxidized Nifedipine; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
381/493
2018-04-14 18:48:42
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 694 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Quantitative value
0.17
Deviation
0.0200000
Metabolite RN
24193057; 576896; 1169840; 497190; 688458; 713494
Metabolite name
(3S)-Hydroxyquinine; alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Oxidized Nifedipine; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 695 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
382/493
2018-04-14 18:48:42
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
458
Deviation
47
Metabolite RN
576896; 1169840; 688458; 713494
Metabolite name
alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 696 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
383/493
2018-04-14 18:48:42
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
293
Deviation
60
Metabolite RN
576896; 1169840; 688458; 713494
Metabolite name
alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 697 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clmax
Unit
µL/min/mg protein
Quantitative value
0.01
Metabolite RN
576896; 1169840; 688458; 713494
Metabolite name
alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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2018-04-14 18:48:42
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 698 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clmax
Unit
µL/min/mg protein
Quantitative value
0.19
Metabolite RN
576896; 1169840; 688458; 713494
Metabolite name
alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 699 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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2018-04-14 18:48:42
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clmax
Unit
µL/min/pmol target
Quantitative value
0.004
Metabolite RN
576896; 1169840; 688458; 713494
Metabolite name
alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 700 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
386/493
2018-04-14 18:48:42
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Clmax
Unit
µL/min/pmol target
Quantitative value
0.012
Metabolite RN
576896; 1169840; 688458; 713494
Metabolite name
alpha-Hydroxytriazolam; 4-Hydroxytriazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857; 1098229; 1727945; 3885115
Concomitants: Compound name
Acetonitrile; Methanol; (+/-)-Isocitric acid; NADP
Concomitants: Compound role
SLV; SLV; COM; COE
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 701 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
pmol/min/1e6 cells
Quantitative value
64
Deviation
36
Metabolite RN
1226636; 568966; 688458; 713494; 751823; 759300
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
387/493
2018-04-14 18:48:42
702 of 976
703 of 976
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857
Concomitants: Compound name
Acetonitrile
Concomitants: Compound role
SLV
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
Vmax
Unit
pmol/min/1e6 cells
Quantitative value
11
Deviation
5
Metabolite RN
1226636; 568966; 688458; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857
Concomitants: Compound name
Acetonitrile
Concomitants: Compound role
SLV
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
388/493
2018-04-14 18:48:42
Target PDB ID
704 of 976
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
nH
Quantitative value
1.4
Metabolite RN
1226636; 568966; 688458; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857
Concomitants: Compound name
Acetonitrile
Concomitants: Compound role
SLV
Bioassay Category
Metabolism/Transport
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
intestine
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Measurement Parameter
fu
Quantitative value
0.94
Galetin, Aleksandra; Houston, J. Brian; Journal of Pharmacology and Experimental Therapeutics; vol. 318; nb. 3; (2006); p. 1220 - 1229, View in Reaxys 705 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
389/493
2018-04-14 18:48:42
706 of 976
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
nH
Quantitative value
1.7
Metabolite RN
1226636; 568966; 688458; 713494; 751823; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; N-Desmethyldiazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857
Concomitants: Compound name
Acetonitrile
Concomitants: Compound role
SLV
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
390/493
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707 of 976
Substance Dose
1 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
76
Deviation
19
Metabolite RN
1226636; 568966; 688458; 713494; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857
Concomitants: Compound name
Acetonitrile
Concomitants: Compound role
SLV
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
S50
Unit
µM
Quantitative value
130
Deviation
86
Metabolite RN
1226636; 568966; 688458; 713494; 759300
Metabolite name
4-Hydroxyalprazolam; alpha-Hydroxyalprazolam; Desmethylflunitrazepam; 3-Hydroxyflunitrazepam; (+/-)-Temazepam
Concomitants: Compound RN
741857
Concomitants: Compound name
Acetonitrile
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
391/493
2018-04-14 18:48:42
Concomitants: Compound role 708 of 976
709 of 976
SLV
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
% Pathway
Unit
%
Qualitative value
>
Quantitative value
75
Metabolite RN
1226636; 713494
Metabolite name
4-Hydroxyalprazolam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
741857
Concomitants: Compound name
Acetonitrile
Concomitants: Compound role
SLV
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Quantitative value
0.29
Deviation
0.200000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 710 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
393/493
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Substance Dose
10 µM
Measurement Parameter
Vmax
Unit
nmol/min/mg protein
Quantitative value
3.2
Deviation
2.20000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 711 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
248
Deviation
78
Measurement pX
3.61
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
394/493
2018-04-14 18:48:42
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 712 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Km
Unit
µM
Quantitative value
208
Deviation
87
Measurement pX
3.68
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 713 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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2018-04-14 18:48:42
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
nH
Quantitative value
1.9
Deviation
0.300000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 714 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
396/493
2018-04-14 18:48:42
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
nH
Quantitative value
1.6
Deviation
0.400000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 715 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clmax
Unit
µL/min/mg protein
Quantitative value
0.71
Deviation
0.640000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
397/493
2018-04-14 18:48:42
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 716 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clmax
Unit
µL/min/mg protein
Quantitative value
5.3
Deviation
4.20000
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 717 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
398/493
2018-04-14 18:48:42
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Quantitative value
1.394
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 718 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology substrate
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
399/493
2018-04-14 18:48:42
Substance Name
Flunitrazepam
Substance Dose
10 µM
Measurement Parameter
Clint
Unit
µL/min/mg protein
Quantitative value
12.9
Metabolite RN
688458; 713494
Metabolite name
Desmethylflunitrazepam; 3-Hydroxyflunitrazepam
Concomitants: Compound RN
1727945; 3885115
Concomitants: Compound name
(+/-)-Isocitric acid; NADP
Concomitants: Compound role
COM; COE
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 719 of 976
Bioassay Category
Metabolism/Transport
Bioassay Name
Binding
Biological Species/NCBI ID
human
Organs/Tissues
liver
Cell Fraction
Microsome
Substance RN
702691View in Reaxys
Substance Name
Flunitrazepam
Substance Dose
1 µM
Measurement Parameter
fu
Quantitative value
0.94
Rawden; Carlile; Tindall; Hallifax; Galetin; Ito; Houston; Xenobiotica; vol. 35; nb. 6; (2005); p. 603 - 625, View in Reaxys 720 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
significant deterioration of the individual optimal quotient in the driver-vehicle-road system and of steering ability, the increase of the number of errors in technical handling; 2 mg; male and female; orally
Measurement Parameter
Qualitative
Schmidt; Brendemuhl; Dellen; Meurer-Kruell; Helm; Arzneimittel-Forschung/Drug Research; vol. 35; nb. 8; (1985); p. 1336 - 1340, View in Reaxys 721 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
impairement of motor coordination - ED 50: 0.23 mg/kg p.o., induce of muscle relaxation - ED 50: 0.15 mg/kg p.o., 0.5 h, female mice; inhibition of in vitro tritiated flunitrazepam binding, Ki = 4.4 nmol/l, rats
Measurement Parameter
Qualitative
Chambon; Perio; Demarne; et al.; Arzneimittel-Forschung/Drug Research; vol. 35; nb. 10; (1985); p. 1572 - 1577, View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
400/493
2018-04-14 18:48:42
722 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : potentiation of GABA-gated currentsBioassay : cells prepared from 8-d-old pups; wholecell recordings of inward currents obtained from granule neurons maintained for 8 d in vitro without flumazenil pretreatment then cells treated with 0.3 μM GABA and various concentrations of flumazenil
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
granule cell
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
in presence of title comp. + 0.3 μM GABA potentiation of GABA-gated currents were 112 percent, 111 percent, 50 percent and 24 percent without flumazenil, in presence of 20 nM, 1 μM and 10 μM flumazenil, resp.
Measurement Parameter
Qualitative
Zheng; Caruncho; Wei Jian Zhu; Vicini; Ikonomovic; Grayson; Costa; Journal of Pharmacology and Experimental Therapeutics; vol. 277; nb. 1; (1996); p. 525 - 533, View in Reaxys 723 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
loss of palpebral reflex (in 77 s), apnea onset (in 91 s), duration of apnea 114 s, percent of apnea onset 80, no changes in PaO2, increase of PaCO2 and pH, decrease of BE, fall in SAP, DAP, MAP (5 min), decrease of HR (human, 0.03 mg/kg)
Measurement Parameter
Qualitative
Morel; Forster; Gardaz; Suter; Gemperle; Arzneimittel-Forschung/Drug Research; vol. 31; nb. 12 a; (1981); p. 2264 - 2267, View in Reaxys 724 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
immediate fall and then slow increase of BP (hypertonic human, 0.015 mg/kg, i.v. + 1 mg/kg of ketamine)
Measurement Parameter
Qualitative
Langrehr; Erdmann; Arzneimittel-Forschung/Drug Research; vol. 31; nb. 12 a; (1981); p. 2269 - 2273, View in Reaxys 725 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Bioassay : binding assay 5-day-old (newborn) rats decapitated, brains rapidly removed, cerebral cortex dissected; membrane susp. incubated 60 min, 4 deg C in Tris-HCl buffer containing 0.5 nmol/l <3H>flunitrazepam and title comp.
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Measurement Parameter
IC50
Unit
nM
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Quantitative value
1.52
Measurement pX
8.82
Giorgi; Lecca; Cancedda; Serra; Corda; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 290; nb. 1; (1995); p. 37 - 47, View in Reaxys 726 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Bioassay : binding assay 5-day-old (newborn) rats decapitated, brains removed, cerebral cortex dissected; membrane susp. incubated in Tris-citrate buffer cont. 1.5-3 nmol/l <3H>t-butylbicyclophosphorothionate (<3H>TBPS), 0.1 nmol/l-30 μmol/l <3H>flunitrazepam and title comp.
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-10 M
Measurement Parameter
IC50
Unit
nM
Quantitative value
2.46
Measurement pX
8.61
Giorgi; Lecca; Cancedda; Serra; Corda; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 290; nb. 1; (1995); p. 37 - 47, View in Reaxys 727 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Bioassay : binding assay 90-day-old (adult) rats decapitated, brains rapidly removed, cerebral cortex dissected; membrane susp. incubated 60 min, 4 deg C in Tris-HCl buffer containing 0.5 nmol/l <3H>flunitrazepam and title comp.
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Measurement Parameter
IC50
Unit
nM
Quantitative value
1.37
Measurement pX
8.86
Giorgi; Lecca; Cancedda; Serra; Corda; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 290; nb. 1; (1995); p. 37 - 47, View in Reaxys 728 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Bioassay : binding assay 90-day-old (adult) rats decapitated, brains removed, cerebral cortex dissected; membrane susp. incubated in Tris-citrate buffer cont. 1.5-3 nmol/l <3H>t-butylbicyclophosphorothionate (<3H>TBPS), 0.1 nmol/l-30 μmol/l <3H>flunitrazepam and title comp.
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-10 M
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Measurement Parameter
IC50
Unit
nM
Quantitative value
7.62
Measurement pX
8.12
Giorgi; Lecca; Cancedda; Serra; Corda; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 290; nb. 1; (1995); p. 37 - 47, View in Reaxys 729 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human embryonic kidney cell line 293 (HEK 293)Bioassay : membranes from HEK cells in Tris-citrate buffer (pH 7.4) incubated with various concns. of (3H)-title comp. for 90 min at 4 deg C in absence or presence of 100 μM diazepam; radioactivity measured after rapid filtration
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0 nM
Qualitative Results
binding of (3H)-title comp. increased with increasing concn.; Kd for specific binding above 100 nM; high values for non specific binding
Measurement Parameter
Qualitative
Fuchs; Zezula; Slany; Sieghart; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 289; nb. 1; (1995); p. 87 - 95, View in Reaxys 730 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : transfected human embryonic kidney (HEK) cell line 293Bioassay : transfected cells, with various combinations of plasmids encoding for GABAA receptor subunits, incubated with (3H)-title comp. in presence or absence of 100 μM GABA for 120 min at 4 deg C; results expressed as percent of the experiments without GABA HEK cells transfected with cDNA encoding for rat brain α1-, α3- and α2-subunits of GABAA receptors; polymerase chain reaction (PCR) used for estimation of
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
2 nM
Qualitative Results
binding of (3)H-title comp. to cells transfected with α1,α3,α2 GABAA subunits in presence of GABA 191 percent; not detected in case of α1α3 and α3α2;
Measurement Parameter
Qualitative
Fuchs; Zezula; Slany; Sieghart; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 289; nb. 1; (1995); p. 87 - 95, View in Reaxys 731 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human embryonic kidney cell line 293 (HEK 293)Bioassay : membranes from HEK cells in Tris-citrate buffer (pH 7.4) incubated with 2 nM (3H)PK 11195 at 4 deg C in absence or presence of 100 μM PK 11195 or various concns. of title comp.
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Measurement Parameter
IC50
Unit
µM
Quantitative value
8.4
Measurement pX
5.08
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Fuchs; Zezula; Slany; Sieghart; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 289; nb. 1; (1995); p. 87 - 95, View in Reaxys 732 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : human embryonic kidney cell line 293 (HEK 293)Bioassay : membranes from HEK cells in Tris-citrate buffer (pH 7.4) incubated with 2 nM (3H)-title comp. at 4 deg C in absence or presence of 100 μM diazepam or various concns. of title comp.
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1.00000E-09 M
Measurement Parameter
IC50
Unit
nM
Quantitative value
120
Measurement pX
6.92
Fuchs; Zezula; Slany; Sieghart; European Journal of Pharmacology - Molecular Pharmacology Section; vol. 289; nb. 1; (1995); p. 87 - 95, View in Reaxys 733 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : bindingTarget : brain of Sprague-Dawley ratBioassay : autoradiographic anal. of dried slices; ASO - antisense oligodeoxynuclide rats (250-300 g) cannulated, injection of vehicle or gamma-2 ASO (18 μg, every 12 h 3 days) into right i.c.v. space; 6 h after last i.c.v. injection rats killing, brain slices prepn.; preincubation (30 min); incubation with 3H-labeled title compd.
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
5 nM
Qualitative Results
specific binding in various brain tissue areas (dependence on gamma-2 ASO pretreatment) evaluated (diagram)
Measurement Parameter
Qualitative
Zhao, Tai-Jun; Li, Ming; Chiu, Ted H.; Rosenberg, Howard C.; Journal of Pharmacology and Experimental Therapeutics; vol. 287; nb. 2; (1998); p. 752 - 759, View in Reaxys 734 of 976
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |antagonist Bioassay : chronic treatment: inhalation of title compd. (120 mg/10 l air per min), pyrazole (1.0 mmol/kg/day, i.p.) and title compd. injections mice (30 g) chronically treated (inhalation) with ethanol and title compd. (8 days), withdrawal (8 h); mice decapitated, lysate from cerebral cortex anal. (Northern blot anal., radioimmunoassay)
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 mg/kg
Substance Route of Adm.
intraperitoneal administration
Qualitative Results
complete abolishment of increase of diazepam binding inhibitor mRNA level in ethanol treated/ withdrawn mice (diagram)
Measurement Parameter
Qualitative
Katsura; Ohkuma; Tsujimura; Kuriyama; Journal of Pharmacology and Experimental Therapeutics; vol. 273; nb. 3; (1995); p. 1529 - 1533, View in Reaxys 735 of 976
Bioassay Category
In Vitro (Efficacy)
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Bioassay Name
In Vitro (others)
Bioassay Details
Effect : expression of α1 subunit protein of GABAA receptorTarget : primary cerebellar granule cells from Sprague-Dawley ratsBioassay : Western blotting; optical density readings in vitro; cells isolated from post-natal day 8 Sprague-Dawley rats, culture density 6-7E6 cells per 25 cm2, treated with the title comp. at day 8 in vitro, 37 deg C, 2 days, enhanced chemiluminescence used to detect GABAA receptor subunit proteins
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 nM
Qualitative Results
the level of GABAA receptor α1 subunit protein is significantly reduced (-25+/-4 percent , -58+/-18 percent, -41+/-3 percent for doses 1 nM, 10 nM, 1μM, respectively
Measurement Parameter
Qualitative
Brown, Maria J.; Bristow, David R.; British Journal of Pharmacology; vol. 118; nb. 5; (1996); p. 1103 - 1110, View in Reaxys 736 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : expression of the GABAA α6 subunit proteinTarget : cerebellar granule cells from SpragueDawley ratsBioassay : BME=basal modified Eagle`s medium, ECL=enhanced cheminiluminescence in vitro; 6-7E6 cells per 25 cm2, at day 8 media replaced with supplemented BME with 10 μM cytosine arabinoside and the title comp., 37 deg C, 12 days, cells harvested for protein, western blotting, ECL detection GABAA receptor subunit proteins
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
expression of GABAA receptor α6 subunit protein is not altered by exposure to 1 μM flunitrazepam treatment over a 12 day treatment period
Measurement Parameter
Qualitative
Brown, Maria J.; Bristow, David R.; British Journal of Pharmacology; vol. 118; nb. 5; (1996); p. 1103 - 1110, View in Reaxys 737 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Target : pancreas of pigBioassay : radioactivity counted after filtration; PK-11195: 1-(2-chlorophenyl-N-methyl-1-methyl-propyl)-3-isoquinolinecarboxamide (benzodiazepine receptor ligand) pure islets of Langerhans prepared from the market-age pigs; membrane prepared and 0.05 mg of proteins incubated at 0 deg C with <3H>PK-11195 (0.4 nmol/l) in the presence of 5 to 8 different concentrations of the title comp.
Substance RN
702691View in Reaxys
Substance Name
31870
Qualitative Results
title comp. displaced PK-11195; Ki = 3400 nmol/l (table)
Measurement Parameter
Qualitative
Marchetti, Piero; Trincavelli, Letizia; Giannarelli, Rosa; Giusti, Laura; Coppelli, Alberto; Martini, Claudia; Navalesi, Renzo; Lucacchini, Antonio; Biochemical Pharmacology; vol. 51; nb. 11; (1996); p. 1437 - 1442, View in Reaxys 738 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Binding
Bioassay Details
Effect : |receptor; binding activity Target : P2 cortical membranesBioassay : CBR tested; nonspecific binding determined in the presence of clonazepam; CBR: central-type benzodiazepine receptor in vitro; saturation binding study; <3H>title comp. as radioligand; incubated at 4 deg C for 30 min under 760 μM O2; liquid scintillation counting
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
7 nM
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Qualitative Results
graphical representation
Measurement Parameter
Qualitative
Courtiere; Molard; Reybaud; Biochemical Pharmacology; vol. 50; nb. 11; (1995); p. 1815 - 1822, View in Reaxys 739 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : |receptor; binding activity Bioassay : in vitro; saturation binding assay; brain membranes from normal or renal dysfunction rats; <3H>title comp. as radioligand; 50 mmol/l Tris-HCl buffer, pH 7.4; 37 deg C; incub. for 30 min; radioligand binding measured by scintillation counting
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.800000 nM
Measurement Parameter
Kd
Unit
nM
Quantitative value
10.52 - 11.69
Measurement pX
7.98
Srivastava, Kalpana; Hatanaka, Tomomi; Katayama, Kazunori; Koizumi, Tamotsu; Biological and Pharmaceutical Bulletin; vol. 22; nb. 3; (1999); p. 288 - 294, View in Reaxys 740 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : receptor subunit protein expression; inhibition ofTarget : rat primary cerebellar granule cellsBioassay : further investigations on mechanism of action using bisindolylmaleimide and H-89 cells incubated with title comp. at 37 deg C for 10 min or 1 h; cells lysed in denaturating buffer, GABAA receptor α1 and α2/3 subunit protein detected in whole cell extracts measuring protein immunoreactivity in grey-scale units by densitometry
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
1 µM
Qualitative Results
reduction of α1 and α2/3 subunit protein levels by 22 and 21 percent, respectively, via protein kinase C-dependent mechanism (diagram)
Measurement Parameter
Qualitative
Johnston, Jonathan D.; Price, Sally A.; Bristow, David R.; British Journal of Pharmacology; vol. 124; nb. 7; (1998); p. 1338 1340, View in Reaxys 741 of 976
Target Name
GABA-A receptor
Target Synonyms
gaba-a receptor; gaba-a receptor activity; ionotropic gaba receptor
Target, Subunit, Species
GABA-A receptor
Target Mutant/Chimera Details
GABA-A receptor:Wild
Target Transfection
Non Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
In Vitro (others)
Bioassay Details
Effect : GABAA receptor; modulation ofBioassay : GABA: α-aminobutyric acid; GABA EC50 ratio: GABA EC50 in presence of title comp./GABA EC50 without title comp.; control: not CHL- or ECHL-enriched or CHL-depleted; CHL: cholesterol; ECHL: epicholesterol neurons enriched with CHL, ECHL or depleted, attached to patch electrode for whole-cell patch clamp recording; membrane current recorded from somata of neurons, voltage-clamped at -20 mV at ca. 20 deg C; GABA and title comp. added simultaneously
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742 of 976
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
nerve cell
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
10 µM
Qualitative Results
GABA EC50 ratio in CHL-depleted (56 percent control), CHL-enriched (182 percent control) and ECHL-incorporated neurons: 0.397, 0.276 and 0.297, resp. (control: 0.477)
Measurement Parameter
Qualitative
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Effect : |pharmacokinetics Bioassay : Cmax: maximum plasma conc.; AUC0-inf: area under conc.time curve from 0 to infinity; Vdα: volume of distribution; CL: plasma clearance title comp. given to 31 neonates and infants (0-438 days) as single 0.2-mg/kg dose (SD, n=28) or multiple doses (MD, 0.2 mg/kg followed by 2-6 doses 0.1 mg/kg, n=3); plasma title comp. conc. measured by GC-MS up to 72 h; pharmacokinetic analysis
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 mg/kg
Substance Route of Adm.
intravenous administration
Qualitative Results
Cmax of title comp. after SD an MD 243 and 161 μg/l, AUC0-inf 2105 and 1454 μg*h/l, Vdα 4.6 and 2.1 l/kg, CL 0.15 and 0.17 l/h/kg, resp.; CL and Vdα sign. increased with postnatal age
Measurement Parameter
Qualitative
Pariente-Khayat, Ann; Treluyer, Jean-Marc; Rey, Elisabeth; Mokhtari, Mostafa; Werner, Evelyne; Jouvet, Philippe; D'Athis, Philippe; Wood, Chantal; Hubert, Philippe; Hotellier, Francoise; Olive, Georges; Pons, Gerard; Clinical Pharmacology and Therapeutics; vol. 66; nb. 2; (1999); p. 136 - 139, View in Reaxys 743 of 976
Substance Effect
Hypotensive
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Bioassay Details
Bioassay : plasma title comp. conc. measured by GC-MS title comp. given to neonates and infants (0-438 days) as single 0.2-mg/kg dose (n=28) or multiple doses (0.2 mg/kg followed by 2-6 doses 0.1 mg/kg, n=3); systolic and diastolic blood pressure (SBP and DBP) and heart rate (HR) recorded up to 72 h
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Name
31870
Substance Dose
0.100000 mg/kg
Substance Route of Adm.
intravenous administration
Qualitative Results
DBP (not SBP and HR) was sign. decreased with increasing title comp. plasma conc.
Measurement Parameter
Qualitative
Pariente-Khayat, Ann; Treluyer, Jean-Marc; Rey, Elisabeth; Mokhtari, Mostafa; Werner, Evelyne; Jouvet, Philippe; D'Athis, Philippe; Wood, Chantal; Hubert, Philippe; Hotellier, Francoise; Olive, Georges; Pons, Gerard; Clinical Pharmacology and Therapeutics; vol. 66; nb. 2; (1999); p. 136 - 139, View in Reaxys 744 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1
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Target Synonyms
bzrap1; kiaa0612; pbr-ip; peripheral benzodiazepine receptor-interacting protein; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimbp1; rims-binding protein 1; tspo-associated protein 1; tspoap1
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards benzodiazepine/GABA A receptor
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
-0.58
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards Gamma-aminobutyric acid A receptor
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
-0.58
Target Name
Gamma-aminobutyric acid receptor subunit delta [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gammaaminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p0c2w5 + p08219
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]; Gamma-aminobutyric acid receptor subunit delta [taurine cattle]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Antagonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Ratio of binding affinity of the compound towards cow Central benzodiazepine receptor from cortical membrane without GABA to that of with 50 uM GABA
Biological Species/NCBI ID
taurine cattle
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748 of 976
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Qualitative value
Not Published
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Antagonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Ratio of binding affinity of the compound towards human Central benzodiazepine receptor from cortical membrane without GABA to that of with 50 uM GABA
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Qualitative value
Not Published
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Toxic activity of compound in mice upon oral administration was determined
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Measurement Parameter
LD50
Unit
mg/kg
Qualitative value
=
Quantitative value
870
Esteki, Mahnaz; Khayamian, Taghi; Chemical Biology and Drug Design; vol. 72; nb. 5; (2008); p. 409 - 435, View in Reaxys 749 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
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Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Efficacy of the compound (1 uM) on percent modulation of human GABAA receptor alpha1beta1epsilon EC20 currents expressed in xenopus oocytes
Biological Species/NCBI ID
human
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Measurement Parameter
%
Unit
%
Measurement Object
Modulation
Qualitative value
=
Quantitative value
0
Deviation
0
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of compound towards human GABAA receptor alpha2beta3gamma2s expressed in xenopus oocytes
Biological Species/NCBI ID
human
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
2.2
Measurement pX
8.66
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Efficacy of the compound (100 x Ki) on percent modulation of human GABAA receptor alpha2beta3gamma2s EC20 currents expressed in xenopus oocytes
Biological Species/NCBI ID
human
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Measurement Parameter
%
Unit
%
Measurement Object
Modulation
Qualitative value
=
Quantitative value
121
Deviation
9.3
Target Name
Cytochrome P450 2B2 [Rattus norvegicus]
Target Synonyms
cyp2b-2; cyp2b2; cypiib2; cytochrome p450 2b2; cytochrome p450 pb4; cytochrome p450e
Target Uniprot ID
p04167
Target, Subunit, Species
Cytochrome P450 2B2 [Rattus norvegicus]
Target Mutant/Chimera Details
Cytochrome P450 2B2 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Bioassay Category
Metabolism/Transport
Bioassay Details
Half maximal activation of PBRU-dependent transcription of a luc expression vector under control of 2.5kb of the CYP2B2 5'-flank, transfected in primary rat hepatocytes upon 48 hr treatment in serum free medium by measuring luciferase activity using Promega dual luciferase kit
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Measurement Parameter
EC50
Unit
µM
Measurement Object
Half maximal activation
Qualitative value
=
Quantitative value
5.7
Deviation
0.5
Measurement pX
5.24
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition constant of the compound against GABAA receptor in C57BL/6J brain membrane in mouse upon incubation for 90 min at 4 degree C using 50 mM Tris/citrate buffer (pH 7.1) and 5 nM [3H]flunitrazepam radioligand
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
7.2
Deviation
0.8
Measurement pX
8.14
Concomitants: Compound name
[3H]Flunitrazepam
Concomitants: Compound role
RAD
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys 754 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dissociation constant of the compound radiolabeled compound with [3H]for GABAA receptor in C57BL/6J brain membrane in mouse
Substance RN
702691View in Reaxys
Measurement Parameter
Kd
Unit
nM
Measurement Object
Dissociation constant
Qualitative value
=
Quantitative value
7.9
Deviation
0.2
Measurement pX
8.1
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys 755 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding maximum of the [3H] radiolabeled compound towards GABAA receptor in gamma2F77I brain membrane in mouse
Substance RN
702691View in Reaxys
Qualitative Results
Binding maximum (Bmax)
Measurement Object
Binding maximum
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys 756 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding maximum of the [3H] radiolabeled compound towards GABAA receptor in C57BL/6J brain membrane in mouse
Substance RN
702691View in Reaxys
Qualitative Results
Binding maximum (Bmax)
Measurement Object
Binding maximum of the [3H] radiolabeled compound towards GABAA receptor
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys 757 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dissociation constant of radiolabbled compound radiolabeled with [3H] towards GABAA receptor in C57BL/6J brain membrane in mouse
Substance RN
702691View in Reaxys
Measurement Parameter
Kd
Unit
nM
Measurement Object
Dissociation constant
Qualitative value
=
Quantitative value
3.8
Deviation
0.4
Measurement pX
8.42
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys
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Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Ratio of inhibition constant against GABAA receptor in C57BL/6J to that of gamma2F77I in mouse brain membrane upon incubation for 90 min at 4 degree C using 50 mM Tris/citrate buffer (pH 7.1) and 5 nM [3H]flunitrazepam radioligand
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Ratio of inhibition constant
Qualitative value
Not Published
Concomitants: Compound name
[3H]Flunitrazepam
Concomitants: Compound role
RAD
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys 759 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition constant of the compound against GABAA receptor in gamma2F77I brain membrane in mouse upon incubation for 90 min at 4 degree C using 50 mM Tris/citrate buffer (pH 7.1) and 5 nM [3H]flunitrazepam radioligand
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
18
Deviation
0.4
Measurement pX
7.74
Concomitants: Compound name
[3H]Flunitrazepam
Concomitants: Compound role
RAD
Ogris, Waltraud; Poeltl, Angelika; Hauer, Birgit; Ernst, Margot; Oberto, Alessandra; Wulff, Peer; Hoeger, Harald; Wisden, William; Sieghart, Werner; Biochemical Pharmacology; vol. 68; nb. 8; (2004); p. 1621 - 1629, View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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763 of 976
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Ratio of maximal velocity of the compound for 3-Hydroxylation reaction mediated by Cytochrome P450 3A5 to that of Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
1.8
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Maximal velocity of the compound for N-Demethylation reaction mediated by Cytochrome P450 3A5 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Qualitative Results
Vmax not calculated
Measurement Parameter
Qualitative
Measurement Object
Vmax
Target Name
Cytochrome P450 3A4
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target, Subunit, Species
Cytochrome P450 3A4
Target Mutant/Chimera Details
Cytochrome P450 3A4:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Intrinsic clearance of the compound for N-Demethylation reaction mediated by Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Clint
Unit
µL/min/nmol P450 all
Quantitative value
0.9
Bioassay Category
Metabolism/Transport
Bioassay Details
Unbound fraction of the compound in cat plasma
Biological Species/NCBI ID
domestic cat
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Quantitative value
0.32
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Lin, Jiunn H.; Current Drug Metabolism; vol. 9; nb. 1; (2008); p. 46 - 59, View in Reaxys 764 of 976
765 of 976
766 of 976
Target Name
Cytochrome P450 3A4
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target, Subunit, Species
Cytochrome P450 3A4
Target Mutant/Chimera Details
Cytochrome P450 3A4:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Intrinsic clearance of the compound for 3-Hydroxylation reaction mediated by Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Clint
Unit
µL/min/nmol P450 all
Quantitative value
5.7
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menten constant of the compound for 3-Hydroxylation mediated by Cytochrome P450 3A5 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Km
Unit
µM
Qualitative value
=
Quantitative value
337
Measurement pX
3.47
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibitory concentration against rat spinal cord benzodiazepine receptor by displacing [3H]RoI5-1788 (0.5 nM) with the compound upon incubation for 90 min
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
spinal cord
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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769 of 976
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
nM
Qualitative value
=
Quantitative value
24
Measurement pX
7.62
Concomitants: Compound name
[3H]Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Intrinsic clearance of the compound for N-Demethylation reaction mediated by Cytochrome P450 3A5 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Clint
Unit
µL/min/nmol P450 all
Quantitative value
1.09
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Ratio of maximal velocity of the compound for N-Demethylation reaction mediated by Cytochrome P450 3A5 to that of Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
0.7
Target Name
Cytochrome P450 3A4
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target, Subunit, Species
Cytochrome P450 3A4
Target Mutant/Chimera Details
Cytochrome P450 3A4:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Maximal velocity of the compound for N-Demethylation reaction mediated by Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Qualitative Results
Vmax not calculated
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772 of 976
Measurement Parameter
Qualitative
Measurement Object
Vmax
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Ratio of intrinsic clearance of the compound for N-Demethylation reaction mediated by Cytochrome P450 3A5 to that of Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
1.2
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Intrinsic clearance of the compound for 3-Hydroxylation reaction mediated by Cytochrome P450 3A5 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Clint
Unit
µL/min/nmol P450 all
Quantitative value
13.4
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards Benzodiazepine receptor type I (BDZR type I) in rat cerebellum was determined
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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773 of 976
774 of 976
775 of 976
Quantitative value
1.5
Measurement pX
8.82
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menten constant of the compound for N-Demethylation mediated by Cytochrome P450 3A5 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Km
Unit
µM
Qualitative value
=
Quantitative value
128
Measurement pX
3.89
Target Name
Cytochrome P450 3A4
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target, Subunit, Species
Cytochrome P450 3A4
Target Mutant/Chimera Details
Cytochrome P450 3A4:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menten constant of the compound for N-Demethylation mediated by Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Km
Unit
µM
Qualitative value
=
Quantitative value
224
Measurement pX
3.65
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Concentration required to displace 0.5 nM (3H)Ro15-1788 from composite binding sites of benzodiazepine receptor in rat spinal cord upon incubation for 90 min
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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777 of 976
778 of 976
Biological Species/NCBI ID
Rattus norvegicus
Organs/Tissues
spinal cord
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
nM
Qualitative value
=
Quantitative value
24
Measurement pX
7.62
Concomitants: Compound name
[3H]Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Maximal velocity of the compound for 3-Hydroxylation reaction mediated by Cytochrome P450 3A5 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Qualitative Results
Vmax not calculated
Measurement Parameter
Qualitative
Measurement Object
Vmax
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Ratio of Michaelis-Menten constant of the compound for N-Demethylation reaction mediated by Cytochrome P450 3A5 to that of Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
0.57
Target Name
Cytochrome P450 3A4
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target, Subunit, Species
Cytochrome P450 3A4
Target Mutant/Chimera Details
Cytochrome P450 3A4:Wild
Bioassay Category
Metabolism/Transport
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Bioassay Details
779 of 976
780 of 976
781 of 976
Maximal velocity of the compound for 3-Hydroxylation reaction mediated by Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Qualitative Results
Vmax not calculated
Measurement Parameter
Qualitative
Measurement Object
Vmax
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards rat cerebellum benzodiazepine receptor was determined
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
cerebellum cell
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
1.5
Measurement pX
8.82
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Ratio of Michaelis-Menten constant of the compound for 3-Hydroxylation reaction mediated by Cytochrome P450 3A5 to that of Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
0.49
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
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783 of 976
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Agonistic activity of the compound against benzodiazepine receptor was determined in rat cerebellar membrane
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
ED50
Unit
nM
Qualitative value
=
Quantitative value
0.007
Measurement pX
11.2
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards benzodiazepine receptor in rat cerebellar membrane at 0 degree C was determined
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
1.49
Deviation
0.09
Measurement pX
8.83
Bioassay Category
Pharmacokinetic
Bioassay Details
Cerebrospinal fluid to plasma concentration ratio of the compound in cat after intravenous dosage
Biological Species/NCBI ID
domestic cat
Substance RN
702691View in Reaxys
Substance Route of Adm.
intravenous administration
Measurement Parameter
CSF/Plasma ratio
Quantitative value
0.34
Lin, Jiunn H.; Current Drug Metabolism; vol. 9; nb. 1; (2008); p. 46 - 59, View in Reaxys
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Bioassay Category
Pharmacokinetic
Bioassay Details
Time taken to reach maximum plasma concentration in cat after intravenous dosage
Biological Species/NCBI ID
domestic cat
Substance RN
702691View in Reaxys
Substance Route of Adm.
intravenous administration
Measurement Parameter
Tmax
Unit
minute
Qualitative value
=
Quantitative value
8.3
Lin, Jiunn H.; Current Drug Metabolism; vol. 9; nb. 1; (2008); p. 46 - 59, View in Reaxys 785 of 976
786 of 976
787 of 976
Target Name
Cytochrome P450 3A5
Target Synonyms
6-beta-testosterone hydroxylase; cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 fa; cytochrome p450 hlp2; cytochrome p450-pcn3
Target, Subunit, Species
Cytochrome P450 3A5
Target Mutant/Chimera Details
Cytochrome P450 3A5:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Ratio of intrinsic clearance of the compound for 3-Hydroxylation reaction mediated by Cytochrome P450 3A5 to that of Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
2.4
Target Name
Cytochrome P450 3A4
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target, Subunit, Species
Cytochrome P450 3A4
Target Mutant/Chimera Details
Cytochrome P450 3A4:Wild
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menten constant of the compound for 3-Hydroxylation mediated by Cytochrome P450 3A4 from Baculovirus-infected insect cells
Substance RN
702691View in Reaxys
Measurement Parameter
Km
Unit
µM
Qualitative value
=
Quantitative value
694
Measurement pX
3.16
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
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789 of 976
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound against Benzodiazepine receptor in rat cerebellar membrane at 0 degree C was determined
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
1.49
Measurement pX
8.83
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1
Target Synonyms
bzrap1; kiaa0612; pbr-ip; peripheral benzodiazepine receptor-interacting protein; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimbp1; rims-binding protein 1; tspo-associated protein 1; tspoap1
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards benzodiazepine receptor
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Qualitative value
Not Published
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
In vitro inhibitory concentration of the compound against growth of isolated Schistosoma mansoni BH from infected mouse portal vein or mesenteric vein upon incubation for 120 h at 37 degree C
Biological Species/NCBI ID
Schistosoma mansoni BH
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
µM
Measurement Object
PARASITE GROWTH
Qualitative value
>
Quantitative value
50
Measurement pX
1
Menezes, Carla M.S.; Rivera, Gildardo; Alves, Marina A.; Do Amaral, Daniel N.; Thibaut, Jean Pierre B.; Noel, Francois; Barreiro, Eliezer J.; Lima, Lidia M.; Chemical Biology and Drug Design; vol. 79; nb. 6; (2012); p. 943 - 949, View in Reaxys 790 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1
Target Synonyms
bzrap1; kiaa0612; pbr-ip; peripheral benzodiazepine receptor-interacting protein; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimbp1; rims-binding protein 1; tspo-associated protein 1; tspoap1
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792 of 976
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of compound towards benzodiazepine receptor in a competition assay
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
-0.58
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of compound against gamma-aminobutyric acid A-benzodiazepine receptor was determined using competition assay against diazepam
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
-0.58
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Percent modulation of human GABAA receptor alpha4beta3delta expressed in mouse L (TK) cells upon incubation with compound at a concentration of 3 uM in 10 mM HEPES, pH 7.3 using 5 mM Mg-ATP measured as maximum current amplitude
Biological Species/NCBI ID
human
Cells/Cell Lines
L-M(TK-) cell line
Substance RN
702691View in Reaxys
Measurement Parameter
%
Unit
%
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794 of 976
Measurement Object
Modulation
Qualitative value
=
Quantitative value
0.75
Deviation
2.8
Concomitants: Compound name
Mg-ATP
Concomitants: Compound role
COM
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Percent modulation of human GABA A receptor alpha 4 beta 3 gamma 2 expressed in mouse L (TK) cells upon incubation with compound at a concentration of 3 uM in 10 mM HEPES, pH 7.3 using 5 mM Mg-ATP measured as maximum current amplitude
Biological Species/NCBI ID
human
Cells/Cell Lines
L-M(TK-) cell line
Substance RN
702691View in Reaxys
Measurement Parameter
%
Unit
%
Measurement Object
Modulation
Qualitative value
=
Quantitative value
-3.1
Deviation
5.7
Concomitants: Compound name
Mg-ATP
Concomitants: Compound role
COM
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lowest toxic dose of the compound in child upon p.o. administration
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
3.478
Measurement Parameter
TDLo
Unit
mg/kg
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Measurement Object
Lowest toxic dose
Quantitative value
3.478
Roald; Dahl; Critical Care Medicine; vol. 17; nb. 12; (1989); p. 1355 - 1356, View in Reaxys 795 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Percent excretion of unchanged compound in urine of human upon oral administration of 1.0 dose
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Measurement Parameter
Ue (%)
Unit
%
Measurement Object
AMOUNT OF UNCHANGED DRUG EXCRETED
Qualitative value
=
Quantitative value
0.5
Benet, Leslie Z.; Broccatelli, Fabio; Oprea, Tudor I.; AAPS Journal; vol. 13; nb. 4; (2011); p. 519 - 547, View in Reaxys 796 of 976
797 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Affinity of the compound towards GABAA receptor alpha1beta3gamma2 was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Unit
nM
Measurement Object
affinity
Qualitative value
=
Quantitative value
3
Bioassay Category
Metabolism/Transport
Bioassay Details
Distribution coefficient of the compound at PH 7.4 (LOG D7.4)
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Qualitative value
Not Published
Benet, Leslie Z.; Broccatelli, Fabio; Oprea, Tudor I.; AAPS Journal; vol. 13; nb. 4; (2011); p. 519 - 547, View in Reaxys 798 of 976
Target Name
Aldo-keto reductase family 1 member C1 [human]
Target Synonyms
20-alpha-hsd; 20-alpha-hydroxysteroid dehydrogenase; akr1c1; aldo-keto reductase family 1 member c1; chlordecone reductase homolog hakrc; dd1/dd2; ddh; ddh1; dihydrodiol dehydrogenase 1/2; hbab; high-affinity hepatic bile acid-binding protein; indanol dehydrogenase; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
Target Uniprot ID
q04828
Target PDB ID
1mrq; 3c3u; 3gug; 3nty; 4yvp
Target, Subunit, Species
Aldo-keto reductase family 1 member C1 [human]
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799 of 976
800 of 976
Target Mutant/Chimera Details
Aldo-keto reductase family 1 member C1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibitory concentration of the compound against human AKR1C1 isoenzyme upon incubation in 0.1 M Potassium phosphate buffer, pH 7.4 at 25 degree C
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
µM
Qualitative value
=
Quantitative value
15
Measurement pX
4.82
Target Name
Aldo-keto reductase family 1 member C2 [human]
Target Synonyms
3-alpha-hsd3; akr1c2; aldo-keto reductase family 1 member c2; chlordecone reductase homolog hakrd; dd-2; dd/babp; dd2; ddh2; dihydrodiol dehydrogenase 2; dihydrodiol dehydrogenase/bile acid-binding protein; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type iii 3-alpha-hydroxysteroid dehydrogenase
Target Uniprot ID
p52895
Target PDB ID
1ihi; 1j96; 1xjb; 2hdj; 2ipj; 4jq1; 4jq2; 4jq3; 4jq4; 4jqa; 4jtq; 4jtr; 4l1w; 4l1x; 4xo6; 4xo7
Target, Subunit, Species
Aldo-keto reductase family 1 member C2 [human]
Target Mutant/Chimera Details
Aldo-keto reductase family 1 member C2 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibitory concentration of the compound against human AKR1C2 isoenzyme upon incubation in 0.1 M Potassium phosphate buffer, pH 7.4 at 25 degree C
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
µM
Qualitative value
=
Quantitative value
13
Measurement pX
4.89
Target Name
Aldo-keto reductase family 1 member C4 [human]
Target Synonyms
3-alpha-hsd1; 3-alpha-hydroxysteroid dehydrogenase type i; akr1c4; aldo-keto reductase family 1 member c4; cdr; chdr; chlordecone reductase; dd-4; dd4; dihydrodiol dehydrogenase 4; hakra
Target Uniprot ID
p17516
Target PDB ID
2fvl
Target, Subunit, Species
Aldo-keto reductase family 1 member C4 [human]
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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801 of 976
802 of 976
Target Mutant/Chimera Details
Aldo-keto reductase family 1 member C4 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibitory concentration of the compound against human AKR1C4 isoenzyme upon incubation in 0.1 M Potassium phosphate buffer, pH 7.4 at 25 degree C
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
µM
Qualitative value
=
Quantitative value
32
Measurement pX
4.49
Target Name
Aldo-keto reductase family 1 member C3 [human]
Target Synonyms
17-beta-hsd 5; 17-beta-hydroxysteroid dehydrogenase type 5; 3-alpha-hsd type 2; 3-alpha-hsd type ii, brain; 3-alpha-hydroxysteroid dehydrogenase type 2; akr1c3; aldo-keto reductase family 1 member c3; chlordecone reductase homolog hakrb; dd-3; dd3; ddh1; dihydrodiol dehydrogenase 3; dihydrodiol dehydrogenase type i; ha1753; hsd17b5; indanol dehydrogenase; kiaa0119; pgfs; prostaglandin f synthase; testosterone 17-beta-dehydrogenase 5; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
Target Uniprot ID
p42330
Target PDB ID
1ry0; 1ry8; 1s1p; 1s1r; 1s2a; 1s2c; 1xf0; 1zq5; 2f38; 2fgb; 3r43; 3r58; 3r6i; 3r7m; 3r8g; 3r8h; 3r94; 3ufy; 3ug8; 3ugr; 3uwe; 4dbs; 4dbu; 4dbw; 4dz5; 4fa3; 4fal; 4fam; 4h7c; 4hmn; 4wdt; 4wdu; 4wdw; 4wdx; 4wrh; 4xvd; 4xve; 4yvv; 4yvx; 4zfc; 5hnt; 5hnu; 5jm5
Target, Subunit, Species
Aldo-keto reductase family 1 member C3 [human]
Target Mutant/Chimera Details
Aldo-keto reductase family 1 member C3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibitory concentration of the compound against human AKR1C3 isoenzyme upon incubation in 0.1 M Potassium phosphate buffer, pH 7.4 at 25 degree C
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
µM
Qualitative value
=
Quantitative value
58
Measurement pX
4.24
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1
Target Synonyms
bzrap1; kiaa0612; pbr-ip; peripheral benzodiazepine receptor-interacting protein; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimbp1; rims-binding protein 1; tspo-associated protein 1; tspoap1
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804 of 976
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1:Wild
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Agonist effect of the compound against anxiolytic response of Benzodiazepine receptor
Substance RN
702691View in Reaxys
Measurement Parameter
MED
Unit
µM/kg
Qualitative value
=
Quantitative value
1.5
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition of [3H]Ro 15-1788 binding in membranes from cells expressing human GABAA receptor alpha(1-5)beta3gamma2; 2.1-4.5 nM
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
4.5
Measurement pX
8.35
Concomitants: Compound name
[3H]Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
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806 of 976
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition of [3H]Ro 15-4513 binding in membranes from cells expressing gamma-aminobutyricacid A receptor alpha6beta3gamma2
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
2000
Measurement pX
5.7
Concomitants: Compound name
[3H]Ro 15-4513
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition of [3H]Ro 15-1788 binding in cells expressing gamma-aminobutyric-acid A receptor alpha(1-5)beta3gamma2; range is 2.1-4.5 nM
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
4.5
Measurement pX
8.35
Concomitants: Compound name
[3H]Ro 15-1788
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
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Target Species (Bioactivity)
human
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition of [3H]Ro 15-4513 in membranes from cells expressing alpha6beta3gamma2 receptor subtype
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
µM
Qualitative value
>
Quantitative value
2
Measurement pX
5.7
Bioassay Category
Pharmacokinetic
Bioassay Details
Percent of compound reaching the systemic circulation after oral administration in humans
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Measurement Parameter
F(%)
Unit
%
Qualitative value
>
Quantitative value
80
Yoshida, Fumitaka; Topliss, John G.; Journal of Medicinal Chemistry; vol. 43; nb. 13; (2000); p. 2575 - 2585, View in Reaxys 808 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Oral lethal dose in rat
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
2.76
Measurement Parameter
LD50
Unit
mg/kg
Quantitative value
2.76
Kiso to Rinsho Clinical Report, 1985, 19, 1277; vol. 19; (1985); p. 1277, View in Reaxys 809 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lowest toxic dose of the compound in infant upon p.o. administration causing dyspnea
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
0.222
Measurement Parameter
TDLo
Unit
mg/kg
Measurement Object
Lowest toxic dose
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Quantitative value
0.222
Gekkan Yakuji. Pharmaceuticals Monthly.,1996, 38, 2597.; vol. 38; (1996); p. 2597, View in Reaxys 810 of 976
811 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Maximum velocity for CYP3A4 catalyzed 3-hydroxyflunitrazepam formation in human liver microsomes
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
19.3
Measurement Parameter
Qualitative
Measurement Object
Vmax
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
In vitro inhibitory concentration of the compound to displace ~1 nM [3H]flunitrazepam from benzodiazepine receptor in rat upon incubation at 0-4 degree C for 60 min
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
nM
Qualitative value
=
Quantitative value
3.8
Measurement pX
8.42
Concomitants: Compound name
[3H]Flunitrazepam
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Concomitants: Compound role 812 of 976
813 of 976
814 of 976
RAD
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Maximum velocity for CYP3A5 catalyzed 3-hydroxyflunitrazepam formation in human liver microsomes
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
3.85
Measurement Parameter
Qualitative
Measurement Object
Vmax
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Maximum velocity for CYP3A4 catalyzed N-desmethylflunitrazepam formation in human liver microsomes
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
0.550
Measurement Parameter
Qualitative
Measurement Object
Vmax
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
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815 of 976
816 of 976
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menton constant for for CYP3A5 catalyzed N-desmethylflunitrazepam formation in human liver microsomes
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
43.8
Measurement Parameter
Km
Unit
µM
Measurement Object
Michaelis Menten constant
Quantitative value
43.8
Measurement pX
4.36
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menton constant for for CYP3A4 catalyzed N-desmethylflunitrazepam formation in human liver microsomes
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
35.6
Measurement Parameter
Km
Unit
µM
Measurement Object
Michaelis Menten constant
Quantitative value
35.6
Measurement pX
4.45
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
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817 of 976
818 of 976
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menton constant for CYP3A4 catalyzed 3-hydroxyflunitrazepam formation in human liver microsomes
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
106
Measurement Parameter
Km
Unit
µM
Measurement Object
Michaelis Menten constant
Quantitative value
106
Measurement pX
3.97
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Michaelis-Menton constant for CYP3A5 catalyzed 3-hydroxyflunitrazepam formation in human liver microsomes
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
49.3
Measurement Parameter
Km
Unit
µM
Measurement Object
Michaelis Menten constant
Quantitative value
49.3
Measurement pX
4.31
Target Name
Cytochrome P450 3A5 [human]
Target Synonyms
cyp3a5; cypiiia5; cytochrome p450 3a5; cytochrome p450 hlp2; cytochrome p450-pcn3
Target Uniprot ID
p20815
Target, Subunit, Species
Cytochrome P450 3A5 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A5 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Maximum velocity for CYP3A5 catalyzed N-desmethylflunitrazepam formation in human liver microsomes
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Biological Species/NCBI ID
819 of 976
human
Substance RN
702691View in Reaxys
Qualitative Results
0.217
Measurement Parameter
Qualitative
Measurement Object
Vmax
Bioassay Category
In Vivo (Animal models)
Bioassay Details
Anticonvulsant activity expressed as the dose which prevented convulsions in 50 of the mice tested after subcutaneous administration of 125 mg/kg was measured by metrazole test
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Route of Adm.
subcutaneous administration
Measurement Parameter
ED50
Unit
mg/kg
Qualitative value
=
Quantitative value
2.41
Blair; Webb; Journal of Medicinal Chemistry; vol. 20; nb. 9; (1977); p. 1206 - 1210, View in Reaxys 820 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Sedation and muscle relaxation effect expressed as dose required to produce relaxation of the body and hind legs when the cats were suspended by the scruff of the neck
Biological Species/NCBI ID
domestic cat
Substance RN
702691View in Reaxys
Measurement Parameter
MED
Unit
mg/kg
Measurement Object
Sedation and muscle relaxation effect
Qualitative value
=
Quantitative value
4.19
Blair; Webb; Journal of Medicinal Chemistry; vol. 20; nb. 9; (1977); p. 1206 - 1210, View in Reaxys 821 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Sedative and muscle relaxant effect expressed as dose which caused three out of six mice to slide off a 70 degree inclined screen
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Measurement Parameter
ED50
Unit
mg/kg
Measurement Object
Sedative and muscle relaxant effect
Qualitative value
=
Quantitative value
2.49
Blair; Webb; Journal of Medicinal Chemistry; vol. 20; nb. 9; (1977); p. 1206 - 1210, View in Reaxys 822 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Taming activity expressed as dose required to tame pairs of fighting mice, their aggression being stimulated by an electrical current applied to their feet using footshock or fighting mice test
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Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Measurement Parameter
MED
Unit
mg/kg
Qualitative value
=
Quantitative value
2.59
Blair; Webb; Journal of Medicinal Chemistry; vol. 20; nb. 9; (1977); p. 1206 - 1210, View in Reaxys 823 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Taming activity expressed as dose required to tame pairs of fighting mice their aggression being stimulated by an electrical current applied to their feet using footshock or fighting mice test
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Measurement Parameter
MED
Unit
mg/kg
Qualitative value
=
Quantitative value
2.59
Blair; Webb; Journal of Medicinal Chemistry; vol. 20; nb. 9; (1977); p. 1206 - 1210, View in Reaxys 824 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Partition Coefficient of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Partition coefficient
Qualitative value
Not Published
Alelyunas, Yun W.; Empfield, James R.; McCarthy, Dennis; Spreen, Russell C.; Bui, Khanh; Pelosi-Kilby, Luciana; Shen, Cindy; Bioorganic and Medicinal Chemistry Letters; vol. 20; nb. 24; (2010); p. 7312 - 7316, View in Reaxys 825 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in rat upon intramuscular administration
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Qualitative Results
> 2000
Measurement Parameter
LD50
Unit
mg/kg
Quantitative value
2000
Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 826 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in rat upon intraperitoneal administration
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Qualitative Results
1060
Measurement Parameter
LD50
Unit
mg/kg
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Quantitative value
1060
Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 827 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in rat upon subcutaneous administration
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Qualitative Results
> 4000
Measurement Parameter
LD50
Unit
mg/kg
Quantitative value
4000
Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 828 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in mouse upon intramuscular administration
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Qualitative Results
> 2000
Measurement Parameter
LD50
Unit
mg/kg
Quantitative value
2000
Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 829 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in mouse upon p.o. administration
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
1200
Measurement Parameter
LD50
Unit
mg/kg
Measurement Object
Lethal dose
Quantitative value
1200
Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 830 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in mouse upon intraperitoneal administration
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Substance Route of Adm.
intraperitoneal administration
Qualitative Results
1050
Measurement Parameter
LD50
Unit
mg/kg
Quantitative value
1050
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 831 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in rat upon p.o. administration
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
415
Measurement Parameter
LD50
Unit
mg/kg
Measurement Object
Lethal dose
Quantitative value
415
Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 832 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lethal dose of the compound in mouse upon subcutaneous administration
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Qualitative Results
> 4000
Measurement Parameter
LD50
Unit
mg/kg
Quantitative value
4000
Kiso to Rinsho. Clinical Report.,1985, 19, 1277.; vol. 19; (1985); p. 1277, View in Reaxys 833 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Plasma half life (elimination)
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
16-35
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
25.5
Clarke's Analysis of Drugs and Poisons, View in Reaxys 834 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Plasma binding of the compound
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
77-80
Measurement Parameter
Protein binding (%)
Unit
%
Measurement Object
Plasma binding
Quantitative value
78.5
Clarke's Analysis of Drugs and Poisons, View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
440/493
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835 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Mean peak plasma concentration after a oral dose daily for 28 days
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
0.0015
Measurement Parameter
Concentration
Unit
µg/mL
Quantitative value
0.0015
Clarke's Analysis of Drugs and Poisons, View in Reaxys 836 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Mean peak plasma concentration after a single oral dose
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
0.0020
Measurement Parameter
Concentration
Unit
µg/mL
Quantitative value
0.002
Clarke's Analysis of Drugs and Poisons, View in Reaxys 837 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Mean peak plasma concentration after a single oral dose
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
0.0025
Measurement Parameter
Concentration
Unit
µg/mL
Quantitative value
0.0025
Clarke's Analysis of Drugs and Poisons, View in Reaxys 838 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Plasma half life (distribution)
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
3
Measurement Parameter
t1/2 el
Unit
hour
Quantitative value
3
Clarke's Analysis of Drugs and Poisons, View in Reaxys 839 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Bioavailability of the compound
Biological Species/NCBI ID
human
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
441/493
2018-04-14 18:48:42
Substance RN
702691View in Reaxys
Qualitative Results
80 - 90
Measurement Parameter
F(%)
Measurement Object
Bioavailability
Qualitative value
@
Clarke's Analysis of Drugs and Poisons, View in Reaxys 840 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Time to peak plasma concentration after a single oral dose
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
1
Measurement Parameter
Tmax
Unit
hour
Quantitative value
1
Clarke's Analysis of Drugs and Poisons, View in Reaxys 841 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Clearance of the compound
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
2
Measurement Parameter
Cl
Unit
mL/min/kg
Quantitative value
2
Clarke's Analysis of Drugs and Poisons, View in Reaxys 842 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Volume of distribution of the compound
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
3.5 - 5.5
Measurement Parameter
Vd
Unit
L/kg
Measurement Object
Volume of distribution
Quantitative value
4.5
Clarke's Analysis of Drugs and Poisons, View in Reaxys 843 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Fecal excretion of the compound in humans
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
442/493
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Qualitative Results
10
Measurement Parameter
Ue (%)
Unit
%
Measurement Object
Excretion
Quantitative value
10
Clarke's Analysis of Drugs and Poisons, View in Reaxys 844 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Oral lethal dose in rat
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Measurement Parameter
LD50
Unit
mg/kg
Qualitative value
=
Quantitative value
415
Kiso to Rinsho, 1985, 19, 1277; vol. 19; (1985); p. 1277, View in Reaxys 845 of 976
Target Name
Platelet-activating factor receptor
Target Synonyms
paf receptor; paf-r; pafr; platelet activating factor receptor; platelet activating factor receptor activity; platelet-activating factor receptor; ptafr
Target, Subunit, Species
Platelet-activating factor receptor
Target Mutant/Chimera Details
Platelet-activating factor receptor:Wild
Substance Action on Target
Antagonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Antagonistic activity towards platelet activating factor receptor
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Qualitative value
Not Published
Patent; Casals-Stenzel, Jorge, Dr.; Weber, Karl-Heinz, Dr.; Walther, Gerhard, Dr.; Harreus, Albrecht, Dr; Muacevic, Gojki, Dr.; EP176928; (A2); (1986), View in Reaxys 846 of 976
Substance Action on Target
Inhibitor
Bioassay Category
In Vivo (Animal models)
Bioassay Details
Inhibitory concentration against PAF-induced bronchoconstriction in guinea pig upon treatment at 1 to ~50 mg/kg (p.o.) or 0.1 - 1.0 mg/kg (i.v.) after 1 hour of intravenous infusion with PAF
Biological Species/NCBI ID
guinea pig
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
µM
Measurement Object
Inhibition of PAF-induced bronchoconstriction in guinea pig
Qualitative value
=
Quantitative value
46
Measurement pX
4.34
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
443/493
2018-04-14 18:48:42
Patent; Casals-Stenzel, Jorge, Dr.; Weber, Karl-Heinz, Dr.; Walther, Gerhard, Dr.; Harreus, Albrecht, Dr; Muacevic, Gojki, Dr.; EP176928; (A2); (1986), View in Reaxys 847 of 976
848 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1
Target Synonyms
bzrap1; kiaa0612; pbr-ip; peripheral benzodiazepine receptor-interacting protein; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimbp1; rims-binding protein 1; tspo-associated protein 1; tspoap1
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1:Wild
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Activity of the compound against Benzodiazepine receptor
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Qualitative value
Not Published
Bioassay Category
Metabolism/Transport
Bioassay Details
In vivo clearance of the compound in human liver microsomes was determined
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Cl
Unit
mL/min/kg
Measurement Object
Clearance
Qualitative value
=
Quantitative value
7.18
Lee, Soyoung; Kim, Dongsup; Archives of Pharmacal Research; vol. 30; nb. 2; (2007); p. 182 - 190, View in Reaxys 849 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
In vivo clearance of the compound in rat liver microsomes was determined
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Cl
Unit
mL/min/kg
Measurement Object
Clearance
Qualitative value
=
Quantitative value
79.17
Lee, Soyoung; Kim, Dongsup; Archives of Pharmacal Research; vol. 30; nb. 2; (2007); p. 182 - 190, View in Reaxys 850 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
In vitro clearance of the compound in rat liver microsomes was determined
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Cl
Unit
mL/min/kg
Measurement Object
Clearance
Qualitative value
=
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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2018-04-14 18:48:42
Quantitative value
70.59
Lee, Soyoung; Kim, Dongsup; Archives of Pharmacal Research; vol. 30; nb. 2; (2007); p. 182 - 190, View in Reaxys 851 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
In vitro clearance of the compound in human liver microsomes was determined
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Cl
Unit
mL/min/kg
Measurement Object
Clearance
Qualitative value
=
Quantitative value
4.03
Lee, Soyoung; Kim, Dongsup; Archives of Pharmacal Research; vol. 30; nb. 2; (2007); p. 182 - 190, View in Reaxys 852 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
In vivo unbound intrinsic clearance of compound in human hepatocytes
Biological Species/NCBI ID
human
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Measurement Parameter
Clint
Unit
mL/min/kg
Qualitative value
=
Quantitative value
12.7
Hallifax, David; Turlizzi, Elisa; Zanelli, Ugo; Houston, J. Brian; European Journal of Pharmaceutical Sciences; vol. 45; nb. 5; (2012); p. 570 - 574, View in Reaxys 853 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Permeability of the compound was calculated
Substance RN
702691View in Reaxys
Measurement Parameter
Papp (transport)
Unit
cm/s
Qualitative value
=
Quantitative value
8.55E-06
Hallifax, David; Turlizzi, Elisa; Zanelli, Ugo; Houston, J. Brian; European Journal of Pharmaceutical Sciences; vol. 45; nb. 5; (2012); p. 570 - 574, View in Reaxys 854 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
In vitro unbound intrinsic clearance of compound in human hepatocytes
Biological Species/NCBI ID
human
Cells/Cell Lines
liver cell
Substance RN
702691View in Reaxys
Measurement Parameter
Clint
Unit
mL/min/kg
Qualitative value
=
Quantitative value
4.5
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
445/493
2018-04-14 18:48:42
Hallifax, David; Turlizzi, Elisa; Zanelli, Ugo; Houston, J. Brian; European Journal of Pharmaceutical Sciences; vol. 45; nb. 5; (2012); p. 570 - 574, View in Reaxys 855 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Partition Coefficient of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Partition coefficient
Qualitative value
Not Published
Dimitra Hadjipavlou-Litina; Corwin Hansch; Chemical reviews; vol. 94; nb. 6; (1994); p. 1483 - 1505, View in Reaxys 856 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Partition Coefficient of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Partition coefficient
Qualitative value
Not Published
Dimitra Hadjipavlou-Litina; Corwin Hansch; Chemical reviews; vol. 94; nb. 6; (1994); p. 1483 - 1505, View in Reaxys 857 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Anticonvulsant activity of compound was evaluated using anti-pentylenetetrazole test
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
2.42
Measurement pX
2.42
Dimitra Hadjipavlou-Litina; Corwin Hansch; Chemical reviews; vol. 94; nb. 6; (1994); p. 1483 - 1505, View in Reaxys 858 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [taurine cattle]
Target Synonyms
peripheral-type benzodiazepine receptor-associated protein 1
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [taurine cattle]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition of specific [3H]RO15-1788 binding to benzodiazepine receptor from bovine brain membranes at 10 uM concentration
Biological Species/NCBI ID
taurine cattle
Substance RN
702691View in Reaxys
Measurement Parameter
pKi
Quantitative value
8.664
Measurement pX
8.66
Concomitants: Compound name
[3H]Ro 15-1788
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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859 of 976
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Anticonvulsant activity of compound was evaluated using anti-pentylenetetrazole test mouse
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
2.42
Measurement pX
2.42
Dimitra Hadjipavlou-Litina; Corwin Hansch; Chemical reviews; vol. 94; nb. 6; (1994); p. 1483 - 1505, View in Reaxys 860 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibition of the binding of [3H]diazepam to Benzodiazepine receptor in homogenates of rat brain cell membranes
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
8.42
Measurement pX
8.42
Concomitants: Compound name
[3H]Diazepam
Concomitants: Compound role
RAD
Dimitra Hadjipavlou-Litina; Corwin Hansch; Chemical reviews; vol. 94; nb. 6; (1994); p. 1483 - 1505, View in Reaxys 861 of 976
Target Name
chloride channel
Target Synonyms
chloride channel; chloride channel activity
Target, Subunit, Species
chloride channel
Target Mutant/Chimera Details
chloride channel:Wild
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Agonistic activity of compound towards chloride channel receptor
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
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862 of 976
Qualitative value
Not Published
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was tested for 50 inhibition of the binding of [3H]diazepam to Benzodiazepine receptor in homogenates of rat brain cell membranes
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
pIC50
Quantitative value
8.42
Measurement pX
8.42
Concomitants: Compound name
[3H]Diazepam
Concomitants: Compound role
RAD
Dimitra Hadjipavlou-Litina; Corwin Hansch; Chemical reviews; vol. 94; nb. 6; (1994); p. 1483 - 1505, View in Reaxys 863 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lowest toxic dose of the compound in women upon p.o. administration
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
0.127
Measurement Parameter
TDLo
Unit
mg/kg
Measurement Object
Lowest toxic dose
Quantitative value
0.127
Hofer; Scollo-Lavizzari; Archives of Internal Medicine; vol. 145; nb. 4; (1985); p. 663 - 664, View in Reaxys 864 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Bound in plasma; 77-79
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
77-79
Measurement Parameter
Protein binding (%)
Unit
%
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Quantitative value
78
Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., 1996; (1996), View in Reaxys 865 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Time to peak plasma concentration in human administered 1 mg orally
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
1.2 +/- 0.8
Measurement Parameter
Tmax
Unit
hour
Quantitative value
1.2
Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., 1996; (1996), View in Reaxys 866 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Volume of distribution
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
intravenous administration
Qualitative Results
3.3 +/- 0.6
Measurement Parameter
Vd
Unit
L/kg
Quantitative value
3.3
Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., 1996; (1996), View in Reaxys 867 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Oral bioavailability
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
~85
Measurement Parameter
F(%)
Unit
%
Quantitative value
85
Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., 1996; (1996), View in Reaxys 868 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Urinary Excretion
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
<1
Measurement Parameter
Qualitative
Measurement Object
Urinary excretion
Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., 1996; (1996), View in Reaxys
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869 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Half life in human
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
15 +/- 5
Measurement Parameter
t1/2 el
Unit
hour
Measurement Object
Half Life
Quantitative value
15
Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., 1996; (1996), View in Reaxys 870 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Clearance of the compound
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
3.5 +/- 0.4
Measurement Parameter
Cl
Unit
mL/min/kg
Quantitative value
3.5
Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th ed., 1996; (1996), View in Reaxys 871 of 976
872 of 976
Target Name
Cytochrome P450 2C9 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cholesterol 25-hydroxylase; cyp2c10; cyp2c9; cypiic9; cytochrome p-450mp; cytochrome p450 2c9; cytochrome p450 mp-4; cytochrome p450 mp-8; cytochrome p450 pb-1; s-mephenytoin 4-hydroxylase
Target Uniprot ID
p11712
Target PDB ID
1og2; 1og5; 1r9o; 4nz2; 5a5i; 5a5j
Target, Subunit, Species
Cytochrome P450 2C9 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C9 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
Metabolism/Transport
Bioassay Details
Percent relative contribution of human CYTOCHROME P450 2C9 to the overall clearance of compound
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
Range = 10 % to 15 %
Measurement Parameter
%
Unit
%
Measurement Object
RELATIVE CONTRIBUTION TO OVERALL CLEARANCE
Quantitative value
10 - 15
Bioassay Category
In Vitro (Efficacy)
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
450/493
2018-04-14 18:48:42
Bioassay Details
Logarithm of effective dose of the compound evaluated using the foot-shock test in mice
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Unit
mM/kg
Qualitative value
=
Quantitative value
2.59
Lukovits; Journal of Medicinal Chemistry; vol. 26; nb. 8; (1983); p. 1104 - 1109, View in Reaxys 873 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Logarithm of effective dose of the compound determined using foot-shock test in mice
Biological Species/NCBI ID
Mus musculus
Substance RN
702691View in Reaxys
Unit
mM/kg
Measurement Object
Logarithm of effective dose
Qualitative value
=
Quantitative value
2.59
Lukovits; Journal of Medicinal Chemistry; vol. 26; nb. 8; (1983); p. 1104 - 1109, View in Reaxys 874 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
In vitro inhibitory activity of the compound (40 uM) against rat brain benzodiazepine receptor using [3H]diazepam (2.6 nM) as radioligand incubated for 90 min at 0 degree C in Tris-HCL cold buffer (50 nM)
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
brain cell
Substance RN
702691View in Reaxys
Substance Dose
= 40 µm
Measurement Parameter
IC50
Unit
µM
Qualitative value
=
Quantitative value
0.005
Deviation
0.0015
Measurement pX
8.3
Concomitants: Compound name
[3H]Diazepam
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Concomitants: Compound role 875 of 976
876 of 976
RAD
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity against benzodiazepine receptor in rat brain membranes using [3H]diazepam as radioligand
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
nM
Qualitative value
=
Quantitative value
5
Measurement pX
8.3
Concomitants: Compound name
[3H]Diazepam
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit delta [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus];+Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18506 + p63138 + p62813
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]; Gamma-aminobutyric acid receptor subunit delta [Rattus norvegicus]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [Rattus norvegicus]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
GABA ratio was calculated between IC50 without GABA and IC50 with GABA
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
1.41
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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878 of 976
879 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Ratio of inhibitory concentration determined without GABA to that of with GABA
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
brain cell
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
1.41
Target Name
Glucocorticoid receptor
Target Synonyms
glucocorticoid receptor; glucocorticoid receptor activity; gr; grl; grl1; nr3c1; nuclear receptor subfamily 3 group c member 1
Target, Subunit, Species
Glucocorticoid receptor
Target Mutant/Chimera Details
Glucocorticoid receptor:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of compound towards Glucocorticoid receptor relative with dexamethasone (Formal charge = neutral)
Substance RN
702691View in Reaxys
Qualitative Results
RBA>1.0 indicates a more active
Measurement Parameter
Binding Score
Qualitative value
@
Bioassay Category
Metabolism/Transport
Bioassay Details
Clearance of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Cl
Unit
mL/min/g
Measurement Object
Clearance
Qualitative value
=
Quantitative value
3.5
Quinones-Torrelo; Martin-Biosca; Martinez-Pla; Sagrado; Villanueva-Camanas; Medina-Hernandez; Mini reviews in medicinal chemistry; vol. 2; nb. 2; (2002); p. 145 - 161, View in Reaxys 880 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
In vitro ability of the compound to displace ~ 1 nM [3H]flunitrazepam from benzodiazepine receptor in rat
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
nM
Qualitative value
=
Quantitative value
3.8
Measurement pX
8.42
Concomitants: Compound name
[3H]Flunitrazepam
Concomitants: Compound role
RAD
Bioassay Category
Metabolism/Transport
Bioassay Details
Volume of distribution of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Vd
Unit
L/kg
Measurement Object
Volume of distribution
Qualitative value
=
Quantitative value
3.3
Quinones-Torrelo; Martin-Biosca; Martinez-Pla; Sagrado; Villanueva-Camanas; Medina-Hernandez; Mini reviews in medicinal chemistry; vol. 2; nb. 2; (2002); p. 145 - 161, View in Reaxys 882 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Renal elimination of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
%
Unit
%
Measurement Object
Renal elimination
Qualitative value
=
Quantitative value
0
Quinones-Torrelo; Martin-Biosca; Martinez-Pla; Sagrado; Villanueva-Camanas; Medina-Hernandez; Mini reviews in medicinal chemistry; vol. 2; nb. 2; (2002); p. 145 - 161, View in Reaxys 883 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Blood-brain distribution measured by LFER model was determined and expressed as log BB (conc. in brain / conc. in blood)
Substance RN
702691View in Reaxys
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Measurement Parameter
Log BB
Measurement Object
Blood-brain distribution
Quantitative value
0.06
Platts, James A.; Abraham, Michael H.; Zhao, Yuan H.; Hersey, Anne; Ijaz, Luke; Butina, Darko; European Journal of Medicinal Chemistry; vol. 36; nb. 9; (2001); p. 719 - 730, View in Reaxys 884 of 976
Bioassay Category
Pharmacokinetic
Bioassay Details
Bioavailability of the compound was determined after oral administration
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Measurement Parameter
F(%)
Unit
%
Measurement Object
Bioavailability
Qualitative value
=
Quantitative value
85
Fecik, Robert A.; Frank, Kristine E.; Gentry, Elmer J.; Menon, Sanjay R.; Mitscher, Lester A.; Telikepalli, Hanumaiah; Medicinal Research Reviews; vol. 18; nb. 3; (1998); p. 149 - 185, View in Reaxys 885 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
In vivo plasma clearance of compound in rat
Biological Species/NCBI ID
Rattus norvegicus
Cells/Cell Lines
plasma cell
Substance RN
702691View in Reaxys
Measurement Parameter
Cl
Unit
mL/min/kg
Qualitative value
=
Quantitative value
47.04
Wan, Hong; Bold, Peter; Larsson, Lars-Olof; Ulander, Johan; Peters, Sheila; Loefberg, Boel; Ungell, Anna-Lena; Nagard, Mats; Llinas, Antonio; Current Drug Metabolism; vol. 11; nb. 7; (2010); p. 583 - 594, View in Reaxys 886 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Bound fraction of compound to rat liver microsomes
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
Bound fraction
Quantitative value
1
Wan, Hong; Bold, Peter; Larsson, Lars-Olof; Ulander, Johan; Peters, Sheila; Loefberg, Boel; Ungell, Anna-Lena; Nagard, Mats; Llinas, Antonio; Current Drug Metabolism; vol. 11; nb. 7; (2010); p. 583 - 594, View in Reaxys 887 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
In vivo intrinsic clearance of compound in rat liver microsomes
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
Clint
Unit
mL/min/kg
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Qualitative value
=
Quantitative value
240
Wan, Hong; Bold, Peter; Larsson, Lars-Olof; Ulander, Johan; Peters, Sheila; Loefberg, Boel; Ungell, Anna-Lena; Nagard, Mats; Llinas, Antonio; Current Drug Metabolism; vol. 11; nb. 7; (2010); p. 583 - 594, View in Reaxys 888 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Unbound fraction of compound to rat liver microsomes
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
fu
Quantitative value
0.25
Wan, Hong; Bold, Peter; Larsson, Lars-Olof; Ulander, Johan; Peters, Sheila; Loefberg, Boel; Ungell, Anna-Lena; Nagard, Mats; Llinas, Antonio; Current Drug Metabolism; vol. 11; nb. 7; (2010); p. 583 - 594, View in Reaxys 889 of 976
890 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Percent change in recombinant GABA A alpha2beta3gamma2s receptor induced GABA currents (Coapplied with EC5¿10 GABA) in xenopus oocytes upon incubation with 1 uM compound
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Qualitative Results
numbers of oocytes tested = 8
Measurement Parameter
%
Unit
%
Measurement Object
Change in response
Qualitative value
=
Quantitative value
234
Deviation
22
Bioassay Category
Metabolism/Transport
Bioassay Details
Tested for blood to plasma ratio of compound in rat
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
BB ratio
Quantitative value
1.2
Wan, Hong; Bold, Peter; Larsson, Lars-Olof; Ulander, Johan; Peters, Sheila; Loefberg, Boel; Ungell, Anna-Lena; Nagard, Mats; Llinas, Antonio; Current Drug Metabolism; vol. 11; nb. 7; (2010); p. 583 - 594, View in Reaxys 891 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
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Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Modulator
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Percent change in recombinant mutant GABA A alpha2beta3gamma2s receptor (SHLA) induced GABA currents (Coapplied with EC5-10 GABA) in xenopus oocytes upon incubation with 1 uM compound
Cells/Cell Lines
oocyte
Substance RN
702691View in Reaxys
Qualitative Results
numbers of oocytes tested = 7
Measurement Parameter
%
Unit
%
Measurement Object
Change in response
Qualitative value
=
Quantitative value
200
Deviation
42
Bioassay Category
Metabolism/Transport
Bioassay Details
Ratio of drug concentration in breast milk to that of maternal plasma was determined
Cells/Cell Lines
plasma cell
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
0.54
Fatemi, Mohammad H.; Ghorbanzad'E, Mehdi; European Journal of Medicinal Chemistry; vol. 45; nb. 11; (2010); p. 5051 5055, View in Reaxys 893 of 976
Target Name
Cytochrome P450 3A4 [human]
Target Synonyms
1,8-cineole 2-exo-monooxygenase; albendazole monooxygenase; albendazole sulfoxidase; cholesterol 25-hydroxylase; cyp3a3; cyp3a4; cypiiia3; cypiiia4; cytochrome p450 3a3; cytochrome p450 3a4; cytochrome p450 hlp; cytochrome p450 nf-25; cytochrome p450-pcn1; nifedipine oxidase; quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase
Target Uniprot ID
p08684
Target PDB ID
1tqn; 1w0e; 1w0f; 1w0g; 2j0d; 2v0m; 3nxu; 3tjs; 3ua1; 4d6z; 4d75; 4d78; 4d7d; 4i3q; 4i4g; 4i4h; 4k9t; 4k9u; 4k9v; 4k9w; 4k9x; 4ny4; 5a1p; 5a1r; 5te8
Target, Subunit, Species
Cytochrome P450 3A4 [human]
Target Mutant/Chimera Details
Cytochrome P450 3A4 [human]:Wild
Target Species (Bioactivity)
human
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Michaelis-Menten constant of the comopund against human liver microsome cytochrome P450 3A4
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
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Measurement Parameter
pKm
Quantitative value
-2.1892
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Oral lowest lethal dose in human
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
0.007
Measurement Parameter
LDLo
Unit
mg/kg
Quantitative value
0.007
Heyndrickx; Journal of Analytical Toxicology; vol. 11; nb. 6; (1987); p. 278 - 278, View in Reaxys 895 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Oral lowest lethal dose in human
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Unit
µg/kg
Qualitative value
=
Quantitative value
430
Heyndrickx; Journal of Analytical Toxicology; vol. 11; nb. 6; (1987); p. 278 - 278, View in Reaxys 896 of 976
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lowest lethal dose of the compound in human upon p.o. administration
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
0.43
Measurement Parameter
LDLo
Unit
mg/kg
Measurement Object
Lowest lethal dose
Quantitative value
0.43
Heyndrickx; Journal of Analytical Toxicology; vol. 11; nb. 6; (1987); p. 278 - 278, View in Reaxys 897 of 976
Substance Effect
Toxic
Bioassay Category
Toxicity/Safety Pharmacology
Bioassay Details
Lowest toxic dose of the compound in human upon p.o. administration
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
oral administration
Qualitative Results
0.184
Measurement Parameter
TDLo
Unit
mg/kg
Measurement Object
Toxic dose
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Quantitative value
0.184
Heyndrickx; Journal of Analytical Toxicology; vol. 11; nb. 6; (1987); p. 278 - 278, View in Reaxys 898 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was evaluated for the Anti-pentylenetetrazole effect
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
Antipent effect
Quantitative value
6.5
Aoyama; Suzuki; Ichikawa; Journal of Medicinal Chemistry; vol. 33; nb. 9; (1990); p. 2583 - 2590, View in Reaxys 899 of 976
900 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
In vitro ability to displace [3H]diazepam from Benzodiazepine receptor in rat cerebral cortex synaptosomal membranes
Biological Species/NCBI ID
Rattus norvegicus
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
nM
Qualitative value
=
Quantitative value
1.5
Measurement pX
8.82
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Synonyms
bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1
Target Uniprot ID
q9jir0
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1 [Rattus norvegicus]:Wild
Target Species (Bioactivity)
Rattus norvegicus
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity was evaluated by testing it for displacement of [3H] diazepam from Benzodiazepine receptor of rat forebrain
Biological Species/NCBI ID
Rattus norvegicus
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Organs/Tissues
brain
Substance RN
702691View in Reaxys
Measurement Parameter
IC50
Unit
nM
Qualitative value
=
Quantitative value
1.5
Measurement pX
8.82
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was evaluated for the Anti-fighting behaviour
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
Antifighting behaviour
Quantitative value
5.8
Aoyama; Suzuki; Ichikawa; Journal of Medicinal Chemistry; vol. 33; nb. 9; (1990); p. 2583 - 2590, View in Reaxys 902 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was evaluated for the Clined screen test
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
Clined screen test
Quantitative value
5.5
Aoyama; Suzuki; Ichikawa; Journal of Medicinal Chemistry; vol. 33; nb. 9; (1990); p. 2583 - 2590, View in Reaxys 903 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Activity of the compound was determined by using Clined screen test
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Quantitative value
5.5
Aoyama; Suzuki; Ichikawa; Journal of Medicinal Chemistry; vol. 33; nb. 9; (1990); p. 2583 - 2590, View in Reaxys 904 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Anti-pentylenetetrazole effect of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Quantitative value
6.5
Aoyama; Suzuki; Ichikawa; Journal of Medicinal Chemistry; vol. 33; nb. 9; (1990); p. 2583 - 2590, View in Reaxys 905 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Anti-fighting behaviour of the compound was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Quantitative value
5.8
Aoyama; Suzuki; Ichikawa; Journal of Medicinal Chemistry; vol. 33; nb. 9; (1990); p. 2583 - 2590, View in Reaxys 906 of 976
Target Name
Cytochrome P450 2C9 [human]
Target Synonyms
(r)-limonene 6-monooxygenase; (s)-limonene 6-monooxygenase; (s)-limonene 7-monooxygenase; cholesterol 25-hydroxylase; cyp2c10; cyp2c9; cypiic9; cytochrome p-450mp; cytochrome p450 2c9; cytochrome p450 mp-4; cytochrome p450 mp-8; cytochrome p450 pb-1; s-mephenytoin 4-hydroxylase
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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907 of 976
Target Uniprot ID
p11712
Target PDB ID
1og2; 1og5; 1r9o; 4nz2; 5a5i; 5a5j
Target, Subunit, Species
Cytochrome P450 2C9 [human]
Target Mutant/Chimera Details
Cytochrome P450 2C9 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Inhibitor
Bioassay Category
Metabolism/Transport
Bioassay Details
In vitro percent inhibition of human liver microsomes Cytochrome P450 2C9 mediated clearance of compound was determined
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Qualitative Results
Range = 10 - 15 %
Measurement Parameter
% Inhibition
Unit
%
Measurement Object
Cytochrome P450 2C9-mediated metabolism
Quantitative value
10 - 15
Bioassay Category
Pharmacokinetic
Bioassay Details
Half life of the compound in human
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Substance Route of Adm.
intravenous administration
Qualitative Results
Range: 18-26 h
Measurement Parameter
t1/2 el
Unit
hour
Qualitative value
=
Quantitative value
26
Mandrioli, Roberto; Mercolini, Laura; Raggi, Maria Augusta; Current drug metabolism; vol. 9; nb. 8; (2008); p. 827 - 844, View in Reaxys 908 of 976
Bioassay Category
Metabolism/Transport
Bioassay Details
Volume of distribution of compound was determined in human
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Vd
Unit
L/kg
Measurement Object
Volume of distribution
Qualitative value
=
Quantitative value
4.58
Sui, Xiaofan; Sun, Jin; Wu, Xin; Li, Haiyan; Liu, Jianfang; He, Zhonggui; Current Drug Metabolism; vol. 9; nb. 6; (2008); p. 574 - 580, View in Reaxys 909 of 976
Bioassay Category
Metabolism/Transport
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Bioassay Details
Volume of distribution of compound was determined in dog
Biological Species/NCBI ID
dog
Substance RN
702691View in Reaxys
Measurement Parameter
Vd
Unit
L/kg
Measurement Object
Volume of distribution
Qualitative value
=
Quantitative value
13
Sui, Xiaofan; Sun, Jin; Wu, Xin; Li, Haiyan; Liu, Jianfang; He, Zhonggui; Current Drug Metabolism; vol. 9; nb. 6; (2008); p. 574 - 580, View in Reaxys 910 of 976
911 of 976
Target Name
Dihydropteridine reductase [Escherichia coli]
Target Synonyms
dihydropteridine reductase; dihydropteridine reductase, nad(p)h-dependent, oxygen-insensitive; nad(p)h nitroreductase; nad(p)h-dependent oxidoreductase; nfnb; oxygen-insensitive nad(p)h nitroreductase
Target Uniprot ID
a0a024l6h0; a0a094vlp5; a0a0b1jr41; a0a0d8w9r9; a0a0l1cbp1; a0a0l7agg6; a0a0t5xnz1; a0a0u4kvs8; c3tkb7; e2qhd8
Target, Subunit, Species
Dihydropteridine reductase [Escherichia coli]
Target Mutant/Chimera Details
Dihydropteridine reductase [Escherichia coli]:Wild
Target Species (Bioactivity)
Escherichia coli
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Ratio of catalytic activity to that of michaelis-menten constant towards recombinant escherichia coli nitroreductase NfsB in the presence of NADPH (2 mM) incubated for 2 h under anaerobic conditions using nitroreductase assay
Biological Species/NCBI ID
Escherichia coli
Substance RN
702691View in Reaxys
Qualitative Results
kcat/Km not calculated
Measurement Parameter
Qualitative
Measurement Object
kcat/Km
Concomitants: Compound name
NADPH (2 mM)
Concomitants: Compound role
COM
Target Name
Dihydropteridine reductase [Escherichia coli]
Target Synonyms
dihydropteridine reductase; dihydropteridine reductase, nad(p)h-dependent, oxygen-insensitive; nad(p)h nitroreductase; nad(p)h-dependent oxidoreductase; nfnb; oxygen-insensitive nad(p)h nitroreductase
Target Uniprot ID
a0a024l6h0; a0a094vlp5; a0a0b1jr41; a0a0d8w9r9; a0a0l1cbp1; a0a0l7agg6; a0a0t5xnz1; a0a0u4kvs8; c3tkb7; e2qhd8
Target, Subunit, Species
Dihydropteridine reductase [Escherichia coli]
Target Mutant/Chimera Details
Dihydropteridine reductase [Escherichia coli]:Wild
Target Species (Bioactivity)
Escherichia coli
Bioassay Category
In Vitro (Efficacy)
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912 of 976
913 of 976
Bioassay Details
Catalytic activity of compound towards recombinant escherichia coli nitroreductase NfsB in the presence of NADPH (2 mM) incubated for 2 h under anaerobic conditions using nitroreductase assay
Biological Species/NCBI ID
Escherichia coli
Substance RN
702691View in Reaxys
Measurement Parameter
kcat
Unit
min-1
Measurement Object
catalytic activity
Qualitative value
=
Quantitative value
7150
Deviation
30
Concomitants: Compound name
NADPH (2 mM)
Concomitants: Compound role
COM
Target Name
Dihydropteridine reductase [Escherichia coli]
Target Synonyms
dihydropteridine reductase; dihydropteridine reductase, nad(p)h-dependent, oxygen-insensitive; nad(p)h nitroreductase; nad(p)h-dependent oxidoreductase; nfnb; oxygen-insensitive nad(p)h nitroreductase
Target Uniprot ID
a0a024l6h0; a0a094vlp5; a0a0b1jr41; a0a0d8w9r9; a0a0l1cbp1; a0a0l7agg6; a0a0t5xnz1; a0a0u4kvs8; c3tkb7; e2qhd8
Target, Subunit, Species
Dihydropteridine reductase [Escherichia coli]
Target Mutant/Chimera Details
Dihydropteridine reductase [Escherichia coli]:Wild
Target Species (Bioactivity)
Escherichia coli
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Activity of compound (64 uM) towards Escherichia coli DH5 nitroreductase NfsB under anaerobic conditions in presence of NADPH (2 mM) using nitroreductase assay
Biological Species/NCBI ID
Escherichia coli DH5
Substance RN
702691View in Reaxys
Qualitative Results
Activity not calculated
Measurement Parameter
Qualitative
Measurement Object
Activity
Concomitants: Compound name
NADPH (2 mM)
Concomitants: Compound role
COM
Target Name
Dihydropteridine reductase [Salmonella enterica serovar Typhimurium]
Target Synonyms
dihydropteridine reductase
Target Uniprot ID
a0a0d6h528
Target, Subunit, Species
Dihydropteridine reductase [Salmonella enterica serovar Typhimurium]
Target Mutant/Chimera Details
Dihydropteridine reductase [Salmonella enterica serovar Typhimurium]:Wild
Target Species (Bioactivity)
Salmonella enterica serovar Typhimurium
Bioassay Category
In Vitro (Efficacy)
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914 of 976
915 of 976
Bioassay Details
Activity of compound (64 uM) towards Salmonella typhimurium TA1537 nitroreductase NfsB under anaerobic conditions in presence of NADPH (2 mM) using nitroreductase assay
Biological Species/NCBI ID
Salmonella enterica serovar Typhimurium TA 1537
Substance RN
702691View in Reaxys
Qualitative Results
Activity not calculated
Measurement Parameter
Qualitative
Measurement Object
Activity
Concomitants: Compound name
NADPH (2 mM)
Concomitants: Compound role
COM
Target Name
Dihydropteridine reductase [Escherichia coli]
Target Synonyms
dihydropteridine reductase; dihydropteridine reductase, nad(p)h-dependent, oxygen-insensitive; nad(p)h nitroreductase; nad(p)h-dependent oxidoreductase; nfnb; oxygen-insensitive nad(p)h nitroreductase
Target Uniprot ID
a0a024l6h0; a0a094vlp5; a0a0b1jr41; a0a0d8w9r9; a0a0l1cbp1; a0a0l7agg6; a0a0t5xnz1; a0a0u4kvs8; c3tkb7; e2qhd8
Target, Subunit, Species
Dihydropteridine reductase [Escherichia coli]
Target Mutant/Chimera Details
Dihydropteridine reductase [Escherichia coli]:Wild
Target Species (Bioactivity)
Escherichia coli
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Maximum velocity of compound towards recombinant escherichia coli nitroreductase NfsB in the presence of NADPH (2 mM) incubated for 2 h under anaerobic conditions using nitroreductase assay
Biological Species/NCBI ID
Escherichia coli
Substance RN
702691View in Reaxys
Measurement Parameter
Vmax
Unit
µM/min
Measurement Object
Maximum velocity
Qualitative value
=
Quantitative value
5960.01
Deviation
190
Concomitants: Compound name
NADPH (2 mM)
Concomitants: Compound role
COM
Target Name
Dihydropteridine reductase [Enterobacter cloacae]
Target Synonyms
dihydropteridine reductase; nad(p)h nitroreductase; nfnb; nitroreductase
Target Uniprot ID
a0a0j0gyz6; a0a0m3fa73; a0a161gis3
Target, Subunit, Species
Dihydropteridine reductase [Enterobacter cloacae]
Target Mutant/Chimera Details
Dihydropteridine reductase [Enterobacter cloacae]:Wild
Target Species (Bioactivity)
Enterobacter cloacae
Bioassay Category
In Vitro (Efficacy)
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916 of 976
917 of 976
Bioassay Details
Activity of compound (64 uM) towards Enterobacter cloacae nitroreductase NfsB under anaerobic conditions in presence of NADPH (2 mM) using nitroreductase assay
Biological Species/NCBI ID
Enterobacter cloacae
Substance RN
702691View in Reaxys
Qualitative Results
Activity not calculated
Measurement Parameter
Qualitative
Measurement Object
Activity
Concomitants: Compound name
NADPH (2 mM)
Concomitants: Compound role
COM
Target Name
Dihydropteridine reductase [Escherichia coli]
Target Synonyms
dihydropteridine reductase; dihydropteridine reductase, nad(p)h-dependent, oxygen-insensitive; nad(p)h nitroreductase; nad(p)h-dependent oxidoreductase; nfnb; oxygen-insensitive nad(p)h nitroreductase
Target Uniprot ID
a0a024l6h0; a0a094vlp5; a0a0b1jr41; a0a0d8w9r9; a0a0l1cbp1; a0a0l7agg6; a0a0t5xnz1; a0a0u4kvs8; c3tkb7; e2qhd8
Target, Subunit, Species
Dihydropteridine reductase [Escherichia coli]
Target Mutant/Chimera Details
Dihydropteridine reductase [Escherichia coli]:Wild
Target Species (Bioactivity)
Escherichia coli
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Michaelis-Menten constant of compound towards recombinant escherichia coli nitroreductase NfsB in the presence of NADPH (2mM) incubated for 2 h under anaerobic conditions using nitroreductase assay
Biological Species/NCBI ID
Escherichia coli
Substance RN
702691View in Reaxys
Measurement Parameter
Km
Unit
µM
Measurement Object
Michaelis-menten constant
Qualitative value
=
Quantitative value
23.17
Deviation
1.6
Measurement pX
4.63
Concomitants: Compound name
NADPH (2 mM)
Concomitants: Compound role
COM
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
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918 of 976
919 of 976
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards GABAA receptor alpha 5 infected in Sf9 cells upon incubation in 50 mM Tris-HCl buffer, pH 7.7 for 90 min at 0 degree C using [3H]-Ro15-4513 (0.5 +/- 1.0 nM) as radioligand with the compound (40 pM to 10 uM) dissolved in 100 ethanol using competition binding assay
Cells/Cell Lines
Sf9
Substance RN
702691View in Reaxys
Substance Dose
= 10 µm
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
5.3
Measurement pX
8.28
Concomitants: Compound name
[3H]Ro 15-4513
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit delta [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gammaaminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p0c2w5 + p08219
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]; Gamma-aminobutyric acid receptor subunit delta [taurine cattle]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Ratio of binding affinity towards Central benzodiazepine receptor in bovine cortical membranes in absence of GABA to that of in presence of 50 uM GABA
Biological Species/NCBI ID
taurine cattle
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
1.68
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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920 of 976
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards Central benzodiazepine receptor in human cortical membrane displacing 0.2 nM [3H]flumazil in 50 mM Tris-citrate buffer at pH 7.4 incubated at 0 degree C for 90 min
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
6.5
Deviation
0.5
Measurement pX
8.19
Concomitants: Compound name
[3H]Flumazenil
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit delta [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gammaaminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p0c2w5 + p08219
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]; Gamma-aminobutyric acid receptor subunit delta [taurine cattle]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards Central benzodiazepine receptor in bovine cortical membrane displacing 0.2 nM [3H]flumazil in 50 mM Tris-citrate buffer at pH 7.4 incubated at 0 degree C for 90 min
Biological Species/NCBI ID
taurine cattle
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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921 of 976
922 of 976
923 of 976
Quantitative value
5.17
Deviation
0.2
Measurement pX
8.29
Concomitants: Compound name
[3H]Flumazenil
Concomitants: Compound role
RAD
Target Name
Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
o14764 + p47870 + p14867
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Ratio of binding affinity towards Central benzodiazepine receptor in human cortical membranes in absence of GABA to that of in presence of 50 uM GABA
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Ratio
Quantitative value
1.6
Target Name
Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dissociation constant of the compound towards GABAA receptor alpha 1 was determined
Substance RN
702691View in Reaxys
Measurement Parameter
Kd
Unit
nM
Qualitative value
=
Quantitative value
4.5
Measurement pX
8.35
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dose required to induce sleep in sleep disorder or minor gynaecological surgery patients was determined
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Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Dose
Unit
mg
Measurement Object
Induce sleep in sleep disorder or minor gynaecological surgery patients
Qualitative value
=
Quantitative value
2
William J. Houlihan; Gregory B. Bennett; Annual reports in medicinal chemistry; vol. 13; (1978); p. 21 - 30, View in Reaxys 924 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dose of the compound required to produce hypnosis in psychiatric patients was determined
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Dose
Unit
mg
Qualitative value
=
Quantitative value
2
Joel G. Berger; Louis C. Iorio; Annual reports in medicinal chemistry; vol. 14; nb. 19; (1979); p. 22 - 30, View in Reaxys 925 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dose required to induce sleep in normal subjects was determined in patients
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Dose
Unit
mg
Measurement Object
Induce sleep in normal subjects
Qualitative value
=
Quantitative value
1
William J. Houlihan; Gregory B. Bennett; Annual reports in medicinal chemistry; vol. 13; (1978); p. 21 - 30, View in Reaxys 926 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was tested for EEG and behavioural effects in monkeys; Effective
Biological Species/NCBI ID
Cercopithecidae
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
EEG and behavioural effects in monkeys
Qualitative value
ND
Charles L. Zirkle; Carl Kaiser; Annual reports in medicinal chemistry; vol. 7; (1972); p. 6 - 17, View in Reaxys 927 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Effect of the compound was determined as antiphotoconvulsive effect; more effective than nitrazepam
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
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Qualitative value
ND
R. Ian Fryer; Annual reports in medicinal chemistry; vol. 6; (1970); p. 1 - 14, View in Reaxys 928 of 976
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards benzodiazepine omega 1 receptor
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
affinity
Quantitative value
2.5
Leslie J. Browne; Kenneth J. Shaw; Annual reports in medicinal chemistry; vol. 26; (1991); p. 1 - 10, View in Reaxys 929 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Effect of the compound was determined as anti-epileptic effect; more effective than nitrazepam
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Qualitative value
ND
R. Ian Fryer; Annual reports in medicinal chemistry; vol. 6; (1970); p. 1 - 14, View in Reaxys 930 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Amount of the compound required to cause hypnosis in man was determined
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Dose
Unit
mg
Qualitative value
=
Quantitative value
2.5
William J. Houlihan; Gregory B. Bennett; Annual reports in medicinal chemistry; vol. 12; (1977); p. 10 - 19, View in Reaxys 931 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Effect of the compound was determined as anti-epileptic effect; more effective than diazepam
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Qualitative value
ND
R. Ian Fryer; Annual reports in medicinal chemistry; vol. 6; (1970); p. 1 - 14, View in Reaxys 932 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Effect of the compound was determined as antiphotoconvulsive effect; more effective than diazepam
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Qualitative value
ND
R. Ian Fryer; Annual reports in medicinal chemistry; vol. 6; (1970); p. 1 - 14, View in Reaxys 933 of 976
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards benzodiazepine omega 2 receptor
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Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
affinity
Quantitative value
1.4
Leslie J. Browne; Kenneth J. Shaw; Annual reports in medicinal chemistry; vol. 26; (1991); p. 1 - 10, View in Reaxys 934 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Amount of the compound required to produce hypnosis in psychiatric patients was determined
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Dose
Unit
mg
Qualitative value
=
Quantitative value
2
Joel G. Berger; Louis C. Iorio; Annual reports in medicinal chemistry; vol. 14; nb. 19; (1979); p. 22 - 30, View in Reaxys 935 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was tested for its hypnotic activity; potent
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
Hypnotic activity
Qualitative value
ND
A.D. Rudzik; W. Friis; Annual reports in medicinal chemistry; vol. 7; (1972); p. 39 - 46, View in Reaxys 936 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gammaaminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p0c2w5 + p08219
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]; Gamma-aminobutyric acid receptor subunit delta [taurine cattle]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
In vitro binding affinity of the compound was evaluated against Gamma-aminobutyric acid A receptor isolated from bovine cerebral cortex by using 3[H] flunitrazepam radioligand; IA denotes inactive
Biological Species/NCBI ID
taurine cattle
Substance RN
702691View in Reaxys
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Measurement Parameter
Activity
Qualitative value
ND
Concomitants: Compound name
[3H]Flunitrazepam
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was tested for hypnotic activity at a dose of 1-3 mg; Effective
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Qualitative value
ND
Marvin Cohen; Annual reports in medicinal chemistry; vol. 11; (1976); p. 13 - 22, View in Reaxys 938 of 976
Target Name
Translocator protein
Target Synonyms
bzrp; ibp; isoquinoline-binding protein; mbr; mitochondrial benzodiazepine receptor; pbr; peripheral-type benzodiazepine receptor; pkbs; translocator protein; tspo
Target, Subunit, Species
Translocator protein
Target Mutant/Chimera Details
Translocator protein:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards benzodiazepine omega 3 receptor
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Measurement Object
affinity
Quantitative value
234
Leslie J. Browne; Kenneth J. Shaw; Annual reports in medicinal chemistry; vol. 26; (1991); p. 1 - 10, View in Reaxys 939 of 976
Target Name
Gamma-aminobutyric acid receptor subunit delta [taurine cattle];+Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle];+Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]
Target Synonyms
gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gammaaminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p0c2w5 + p08219
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]; Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]; Gamma-aminobutyric acid receptor subunit delta [taurine cattle]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit delta [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [taurine cattle]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
In vitro binding affinity of the compound was evaluated against GABA receptor isolated from bovine cerebral cortex by using 3[H] flunitrazepam radioligand; IA denotes inactive
Biological Species/NCBI ID
taurine cattle
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Substance RN
702691View in Reaxys
Qualitative Results
IA
Measurement Parameter
Activity
Qualitative value
NA
Measurement pX
1
Concomitants: Compound name
[3H]Flunitrazepam
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was tested for EEG and behavioral effects in monkeys; Effective
Biological Species/NCBI ID
Cercopithecidae
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Qualitative value
ND
Charles L. Zirkle; Carl Kaiser; Annual reports in medicinal chemistry; vol. 7; (1972); p. 6 - 17, View in Reaxys 941 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Compound was tested for its effect as an hypnotic agent; effective at a dose of 1-3 mg
Substance RN
702691View in Reaxys
Measurement Parameter
Activity
Qualitative value
ND
Marvin Cohen; Annual reports in medicinal chemistry; vol. 11; (1976); p. 13 - 22, View in Reaxys 942 of 976
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Dose required to induce sleep in normal subjects was determined
Biological Species/NCBI ID
human
Substance RN
702691View in Reaxys
Measurement Parameter
Dose
Unit
mg
Qualitative value
=
Quantitative value
1
William J. Houlihan; Gregory B. Bennett; Annual reports in medicinal chemistry; vol. 13; (1978); p. 21 - 30, View in Reaxys 943 of 976
Target Name
Peripheral-type benzodiazepine receptor-associated protein 1
Target Synonyms
bzrap1; kiaa0612; pbr-ip; peripheral benzodiazepine receptor-interacting protein; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimbp1; rims-binding protein 1; tspo-associated protein 1; tspoap1
Target, Subunit, Species
Peripheral-type benzodiazepine receptor-associated protein 1
Target Mutant/Chimera Details
Peripheral-type benzodiazepine receptor-associated protein 1:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Tested for binding affinity of the compound against benzodiazepine receptor sites in Ligand/ [3H]FLU competition experiment in presence of GABA; Causes a moderate increase in affinity
Substance RN
702691View in Reaxys
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Qualitative Results
Increase in affinity
Measurement Parameter
Activity
Qualitative value
@
Concomitants: Compound name
GABA; [3H]FLU
Concomitants: Compound role
COM; RAD
Bioassay Category
In Vivo (Animal models)
Bioassay Name
In vivo Measurement
Biological Species/NCBI ID
rhesus monkey
Substance RN
702691View in Reaxys
Substance Name
figures 1; 4; 5, page 185, paragraphs 2+4; table 1 - compound: flunitrazepam
Substance Route of Adm.
subcutaneous administration
Substance Dosing Regimen
Single
Measurement Parameter
ED50
Unit
mg/kg
Quantitative value
0.11
Deviation
0.100000
Gerak, Lisa R.; France, Charles P.; Psychopharmacology; vol. 231; nb. 1; (2014); p. 181 - 190, View in Reaxys 945 of 976
946 of 976
Target Name
Carboxylesterase 1 [human]
Target Synonyms
carboxylesterase 1
Target, Subunit, Species
Carboxylesterase 1 [human]
Target Mutant/Chimera Details
Carboxylesterase 1 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
Sf21 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
48
Measurement pX
3.97
Concomitants: Compound RN
1281877
Concomitants: Compound name
4-Nitrophenyl acetate
Concomitants: Compound role
SUB
Target Name
Carboxylesterase 2 [human]
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Target Synonyms
carboxylesterase 2
Target, Subunit, Species
Carboxylesterase 2 [human]
Target Mutant/Chimera Details
Carboxylesterase 2 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
Metabolism/Transport
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
Sf21 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
42
Measurement pX
3.86
Concomitants: Compound RN
1281877
Concomitants: Compound name
4-Nitrophenyl acetate
Concomitants: Compound role
SUB
Target Name
Arylacetamide deacetylase [human]
Target Synonyms
aadac; arylacetamide deacetylase; dac
Target Uniprot ID
p22760
Target, Subunit, Species
Arylacetamide deacetylase [human]
Target Mutant/Chimera Details
Arylacetamide deacetylase [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Bioassay Category
In Vitro (Efficacy)
Bioassay Name
Enzymology inhibition
Cells/Cell Lines
Sf21 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
100 µM
Measurement Parameter
% Inhibition
Qualitative value
NA
Measurement pX
1
Concomitants: Compound RN
1281877
Concomitants: Compound name
4-Nitrophenyl acetate
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Concomitants: Compound role
SUB
Bioassay Category
In Vitro (Efficacy)
Cells/Cell Lines
THP-1 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
10 μM
Measurement Parameter
% increase
Measurement Object
[3H]cholesterol efflux
Qualitative value
Active
Statistical sgnificance (Qualitative)
NS
Measurement pX
5
Patent; GLASGOW CALEDONIAN UNIVERSITY; GRAHAM, Annette; WO2015/25180; (2015); (A1) English, View in Reaxys 949 of 976
Bioassay Category
In Vitro (Efficacy)
Cells/Cell Lines
THP-1 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
10 μM
Measurement Parameter
% increase
Measurement Object
[3H]cholesterol efflux
Qualitative value
Active
Statistical sgnificance (Qualitative)
S
Measurement pX
5
Concomitants: Compound RN
10148999
Concomitants: Compound name
apolipoprotein A-I
Concomitants: Compound role
COM
Patent; GLASGOW CALEDONIAN UNIVERSITY; GRAHAM, Annette; WO2015/25180; (2015); (A1) English, View in Reaxys 950 of 976
Target Name
Solute carrier family 22 member 5 [human]
Target Synonyms
high-affinity sodium-dependent carnitine cotransporter; octn2; organic cation/carnitine transporter 2; slc22a5; solute carrier family 22 member 5
Target Uniprot ID
o76082
Target, Subunit, Species
Solute carrier family 22 member 5 [human]
Target Mutant/Chimera Details
Solute carrier family 22 member 5 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Inhibitor
Bioassay Category
Metabolism/Transport
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952 of 976
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
200 μM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
92.9
Measurement pX
4.82
Concomitants: Compound RN
606492
Concomitants: Compound name
metformin
Concomitants: Compound role
SUB
Target Name
Solute carrier family 22 member 5 [human]
Target Synonyms
high-affinity sodium-dependent carnitine cotransporter; octn2; organic cation/carnitine transporter 2; slc22a5; solute carrier family 22 member 5
Target Uniprot ID
o76082
Target, Subunit, Species
Solute carrier family 22 member 5 [human]
Target Mutant/Chimera Details
Solute carrier family 22 member 5 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Inhibitor
Bioassay Category
Metabolism/Transport
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
20 μM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
74.1
Statistical sgnificance (Qualitative)
S
Measurement pX
5.16
Concomitants: Compound RN
606492
Concomitants: Compound name
metformin
Concomitants: Compound role
SUB
Target Name
Solute carrier family 22 member 5 [human]
Target Synonyms
high-affinity sodium-dependent carnitine cotransporter; octn2; organic cation/carnitine transporter 2; slc22a5; solute carrier family 22 member 5
Target Uniprot ID
o76082
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Target, Subunit, Species
Solute carrier family 22 member 5 [human]
Target Mutant/Chimera Details
Solute carrier family 22 member 5 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
200 μM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
57.5
Measurement pX
3.83
Concomitants: Compound RN
1618932
Concomitants: Compound name
1-methyl-4-phenylpyridinium
Concomitants: Compound role
SUB
Target Name
Solute carrier family 22 member 5 [human]
Target Synonyms
high-affinity sodium-dependent carnitine cotransporter; octn2; organic cation/carnitine transporter 2; slc22a5; solute carrier family 22 member 5
Target Uniprot ID
o76082
Target, Subunit, Species
Solute carrier family 22 member 5 [human]
Target Mutant/Chimera Details
Solute carrier family 22 member 5 [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Transfected
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
20 μM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
5.1
Measurement pX
1
Concomitants: Compound RN
1618932
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Concomitants: Compound name
1-methyl-4-phenylpyridinium
Concomitants: Compound role
SUB
Target Name
UDP-glucuronosyltransferase 1-8
Target Synonyms
a3; gnt1; udp-glucuronosyltransferase 1-8; udp-glucuronosyltransferase 1-h; udp-glucuronosyltransferase 1a8; udpgt 1-8; ugt-1h; ugt1; ugt1*8; ugt1-08; ugt1.8; ugt1a8; ugt1h
Target, Subunit, Species
UDP-glucuronosyltransferase 1-8
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-8:Wild
Target Transfection
Transfected
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
20 μM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
14
Measurement pX
1
Concomitants: Compound RN
4890356; 78881
Concomitants: Compound role
SUB; COM
Oda, Shingo; Fujiwara, Ryoichi; Kutsuno, Yuki; Fukami, Tatsuki; Itoh, Tomoo; Yokoi, Tsuyoshi; Nakajima, Miki; Drug Metabolism and Disposition; vol. 43; nb. 6; (2015); p. 812 - 818, View in Reaxys 955 of 976
Target Name
UDP-glucuronosyltransferase 1-8
Target Synonyms
a3; gnt1; udp-glucuronosyltransferase 1-8; udp-glucuronosyltransferase 1-h; udp-glucuronosyltransferase 1a8; udpgt 1-8; ugt-1h; ugt1; ugt1*8; ugt1-08; ugt1.8; ugt1a8; ugt1h
Target, Subunit, Species
UDP-glucuronosyltransferase 1-8
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-8:Wild
Target Transfection
Transfected
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
20 μM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
19
Measurement pX
1
Concomitants: Compound RN
4890356; 78881
Concomitants: Compound role
SUB; COM
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Oda, Shingo; Fujiwara, Ryoichi; Kutsuno, Yuki; Fukami, Tatsuki; Itoh, Tomoo; Yokoi, Tsuyoshi; Nakajima, Miki; Drug Metabolism and Disposition; vol. 43; nb. 6; (2015); p. 812 - 818, View in Reaxys 956 of 976
Target Name
UDP-glucuronosyltransferase 1-8
Target Synonyms
a3; gnt1; udp-glucuronosyltransferase 1-8; udp-glucuronosyltransferase 1-h; udp-glucuronosyltransferase 1a8; udpgt 1-8; ugt-1h; ugt1; ugt1*8; ugt1-08; ugt1.8; ugt1a8; ugt1h
Target, Subunit, Species
UDP-glucuronosyltransferase 1-8
Target Mutant/Chimera Details
UDP-glucuronosyltransferase 1-8:Wild
Target Transfection
Transfected
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
20 μM
Measurement Parameter
% Inhibition
Unit
%
Quantitative value
30
Measurement pX
4.33
Concomitants: Compound RN
4890356; 78881
Concomitants: Compound role
SUB; COM
Oda, Shingo; Fujiwara, Ryoichi; Kutsuno, Yuki; Fukami, Tatsuki; Itoh, Tomoo; Yokoi, Tsuyoshi; Nakajima, Miki; Drug Metabolism and Disposition; vol. 43; nb. 6; (2015); p. 812 - 818, View in Reaxys 957 of 976
958 of 976
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2;+Gamma-aminobutyric acid receptor subunit beta-3;+Gamma-aminobutyric acid receptor subunit alpha-2
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-2; gabra-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2; Gamma-aminobutyric acid receptor subunit beta-3; Gamma-aminobutyric acid receptor subunit gamma-2
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2:Wild + Gamma-aminobutyric acid receptor subunit beta-3:Wild + Gamma-aminobutyric acid receptor subunit alpha-2:Wild
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards GABA-A receptor alpha2beta3gamma2 using [3H]Ro 15-1788 as radioligand incubated in 10 mM Tris-Hcl for 1 h at RT with pH 7.4
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
2.5
Measurement pX
8.6
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2;+Gamma-aminobutyric acid receptor subunit beta-3;+Gamma-aminobutyric acid receptor subunit alpha-3
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Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-3; gabra-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3; Gamma-aminobutyric acid receptor subunit beta-3; Gamma-aminobutyric acid receptor subunit gamma-2
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2:Wild + Gamma-aminobutyric acid receptor subunit beta-3:Wild + Gamma-aminobutyric acid receptor subunit alpha-3:Wild
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards GABA-A receptor alpha3beta3gamma2 using [3H]Ro 15-1788 as radioligand incubated in 10 mM Tris-Hcl for 1 h at RT with pH 7.4
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
4.8
Measurement pX
8.32
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2;+Gamma-aminobutyric acid receptor subunit beta-3;+Gamma-aminobutyric acid receptor subunit alpha-1
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-3; Gamma-aminobutyric acid receptor subunit gamma-2
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2:Wild + Gamma-aminobutyric acid receptor subunit beta-3:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards GABA-A receptor alpha1beta3gamma2 using [3H]Ro 15-1788 as radioligand incubated in 10 mM Tris-Hcl for 1 h at RT with pH 7.4
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
5.2
Measurement pX
8.28
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2;+Gamma-aminobutyric acid receptor subunit beta-3;+Gamma-aminobutyric acid receptor subunit alpha-5
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5; Gamma-aminobutyric acid receptor subunit beta-3; Gamma-aminobutyric acid receptor subunit gamma-2
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961 of 976
962 of 976
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2:Wild + Gamma-aminobutyric acid receptor subunit beta-3:Wild + Gamma-aminobutyric acid receptor subunit alpha-5:Wild
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards GABA-A receptor alpha5beta3gamma2 using [3H]Ro 15-1788 as radioligand incubated in 10 mM Tris-Hcl for 1 h at RT with pH 7.4
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
=
Quantitative value
2.11
Measurement pX
8.68
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2;+Gamma-aminobutyric acid receptor subunit beta-3;+Gamma-aminobutyric acid receptor subunit alpha-6
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-6; Gamma-aminobutyric acid receptor subunit beta-3; Gamma-aminobutyric acid receptor subunit gamma-2
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2:Wild + Gamma-aminobutyric acid receptor subunit beta-3:Wild + Gamma-aminobutyric acid receptor subunit alpha-6:Wild
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity of the compound towards GABA-A receptor alpha6beta3gamma2 using [3H]Ro 15-4513 as radioligand incubated in 10 mM Tris-Hcl for 1 h at RT with pH 7.4
Substance RN
702691View in Reaxys
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
10
Measurement pX
8
Target Name
GABA-A receptor
Target Synonyms
gaba-a receptor; gaba-a receptor activity; ionotropic gaba receptor
Target, Subunit, Species
GABA-A receptor
Target Mutant/Chimera Details
GABA-A receptor:Wild
Substance Action on Target
Agonist
Substance Effect
agonistic activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Agonistic activity of the compound towards GABAA receptor
Substance RN
702691View in Reaxys
Qualitative Results
Not Published
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964 of 976
Measurement Parameter
Qualitative
Qualitative value
Not Published
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-2 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2
Target Uniprot ID
p18507 + p28472 + p47869
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-2 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards gamma-aminobutyric acid (GABA) A receptor, alpha2beta3gamma2 with 10 mM Tris-HCl pH 7.4 using [3H]Ro15-1788 upon incubation for 1 h at RT
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Ki
Qualitative value
Not Published
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-5 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5
Target Uniprot ID
p18507 + p28472 + p31644
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-5 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards gamma-aminobutyric acid (GABA) A receptor, alpha5beta3gamma2 with 10 mM Tris-HCl pH 7.4 using [3H]Ro15-1788 upon incubation for 1 h at RT
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Ki
Qualitative value
Not Published
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966 of 976
967 of 976
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-3 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3
Target Uniprot ID
p18507 + p28472 + p34903
Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-3 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards gamma-aminobutyric acid (GABA) A receptor, alpha3beta3gamma2 using with 10 mM Tris-HCl pH 7.4 [3H]Ro15-1788 upon incubation for 1 h at RT using Tris-HCl as radioligand AT pH 7.5
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Ki
Qualitative value
Not Published
Target Name
Gamma-aminobutyric acid receptor subunit beta-3;+Gamma-aminobutyric acid receptor subunit alpha-6;+GABA-A receptor (Gamma 3)
Target Synonyms
gaba(a) receptor subunit beta-3; gabrb-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba-a receptor (gamma 3)
Target, Subunit, Species
GABA-A receptor (Gamma 3); Gamma-aminobutyric acid receptor subunit alpha-6; Gamma-aminobutyric acid receptor subunit beta-3
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit beta-3:Wild + Gamma-aminobutyric acid receptor subunit alpha-6:Wild + GABA-A receptor (Gamma 3):Wild
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards gamma-aminobutyric acid (GABA) A receptor, alpha6beta3gamma3 using with 10 mM Tris-HCl pH 7.4 [3H]Ro15-1788 upon incubation for 1 h at RT
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Ki
Qualitative value
Not Published
Target Name
Gamma-aminobutyric acid receptor subunit gamma-2 [human];+Gamma-aminobutyric acid receptor subunit beta-3 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]
Target Synonyms
gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-3; gabrb3; gamma-aminobutyric acid receptor subunit beta-3 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1
Target Uniprot ID
p18507 + p28472 + p14867
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Target PDB ID
4cof
Target, Subunit, Species
Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-3 [human]; Gamma-aminobutyric acid receptor subunit gamma-2 [human]
Target Mutant/Chimera Details
Gamma-aminobutyric acid receptor subunit gamma-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-3 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild
Target Species (Bioactivity)
human
Substance Action on Target
Radioligand (/ligand)
Substance Effect
binding activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Binding affinity towards gamma-aminobutyric acid (GABA) A receptor, alpha1beta3gamma2 with 10 mM Tris-HCl pH 7.4 using [3H]Ro15-1788 as radioligand upon incubation for 1 h at RT
Substance RN
702691View in Reaxys
Measurement Parameter
Qualitative
Measurement Object
Ki
Qualitative value
Not Published
Target Name
GABA-A receptor
Target Synonyms
gaba-a receptor; gaba-a receptor activity; ionotropic gaba receptor
Target, Subunit, Species
GABA-A receptor
Target Mutant/Chimera Details
GABA-A receptor:Wild
Substance Action on Target
Inhibitor
Substance Effect
inhibitory activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Inhibitory activity of the compound against GABAA receptor
Substance RN
702691View in Reaxys
Qualitative Results
Not Published
Measurement Parameter
Qualitative
Qualitative value
Not Published
Target Name
Protein kinase C epsilon type
Target Synonyms
protein kinase c epsilon type
Target, Subunit, Species
Protein kinase C epsilon type
Target Mutant/Chimera Details
Protein kinase C epsilon type:Wild
Substance Action on Target
Inhibitor
Substance Effect
inhibitory activity
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
Effective concentration of compound required for modulation of GABA-stimulated currents in HEK 293 cell line expressing PROTEIN KINASE C EPSILON using ELISA method
Cells/Cell Lines
HEK293 cell line
Substance RN
702691View in Reaxys
Measurement Parameter
EC50
Unit
nM
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971 of 976
Measurement Object
Gaba-Stimulated Currents
Qualitative value
=
Quantitative value
150
Measurement pX
6.82
Target Name
Translocator protein
Target Synonyms
bzrp; ibp; isoquinoline-binding protein; mbr; mitochondrial benzodiazepine receptor; pbr; peripheral-type benzodiazepine receptor; pkbs; translocator protein; tspo
Target, Subunit, Species
Translocator protein
Target Mutant/Chimera Details
Translocator protein:Wild
Substance Action on Target
Radioligand (/ligand)
Bioassay Category
In Vitro (Efficacy)
Cells/Cell Lines
C6
Cell Fraction
Lysate
Substance RN
702691View in Reaxys
Substance Name
3H-flunitrazepam
Measurement Parameter
Ki
Unit
nM
Qualitative value
>
Quantitative value
10000
Measurement pX
1
Concomitants: Compound RN
4264456
Concomitants: Compound name
%3&H-PK 11195
Concomitants: Compound role
RAD
Bioassay Category
In Vitro (Efficacy)
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
cerebellum
Cells/Cell Lines
granule cell
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.1 μM
Measurement Parameter
% Efficacy
Unit
%
Measurement Object
chloride channel conductance
Quantitative value
17
Concomitants: Compound RN
906818
Concomitants: Compound name
4-aminobutyric acid
Concomitants: Compound role
IND
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Gatta, Elena; Cupello, Aroldo; Di Braccio, Mario; Grossi, Giancarlo; Robello, Mauro; Scicchitano, Francesca; Russo, Emilio; De Sarro, Giovambattista; Journal of Molecular Neuroscience; vol. 60; nb. 4; (2016); p. 539 - 547, View in Reaxys 972 of 976
Substance Effect
Anticonvulsant
Bioassay Category
In Vivo (Animal models)
Biological Species/NCBI ID
DBA 2 mouse
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.11 - 0.41 μmol/kg
Substance Route of Adm.
intraperitoneal administration
Measurement Parameter
ED50
Unit
μmol/kg
Measurement Object
ED 50 for clonic phase
Quantitative value
0.22
Gatta, Elena; Cupello, Aroldo; Di Braccio, Mario; Grossi, Giancarlo; Robello, Mauro; Scicchitano, Francesca; Russo, Emilio; De Sarro, Giovambattista; Journal of Molecular Neuroscience; vol. 60; nb. 4; (2016); p. 539 - 547, View in Reaxys 973 of 976
Substance Effect
Anticonvulsant
Bioassay Category
In Vivo (Animal models)
Biological Species/NCBI ID
DBA 2 mouse
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Substance Dose
0.09 - 0.36 μmol/kg
Substance Route of Adm.
intraperitoneal administration
Measurement Parameter
ED50
Unit
μmol/kg
Measurement Object
ED50 for Tonic phase
Quantitative value
0.18
Gatta, Elena; Cupello, Aroldo; Di Braccio, Mario; Grossi, Giancarlo; Robello, Mauro; Scicchitano, Francesca; Russo, Emilio; De Sarro, Giovambattista; Journal of Molecular Neuroscience; vol. 60; nb. 4; (2016); p. 539 - 547, View in Reaxys 974 of 976
Target Name
GABA-A receptor [Sprague Dawley rat]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [Sprague Dawley rat]
Target Mutant/Chimera Details
GABA-A receptor [Sprague Dawley rat]:Wild
Target Species (Bioactivity)
Sprague Dawley rat
Target Transfection
Non Transfected
Substance Action on Target
Agonist
Bioassay Category
In Vitro (Efficacy)
Biological Species/NCBI ID
Sprague Dawley rat
Organs/Tissues
brain
Cells/Cell Lines
Synaptosomal cell
Substance RN
702691View in Reaxys
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976 of 976
Substance Name
flunitrazepam
Measurement Parameter
% Increase
Measurement Object
chloride uptake
Qualitative value
Active
Target Name
GABA-A receptor [taurine cattle]
Target Synonyms
gaba-a receptor
Target, Subunit, Species
GABA-A receptor [taurine cattle]
Target Mutant/Chimera Details
GABA-A receptor [taurine cattle]:Wild
Target Species (Bioactivity)
taurine cattle
Target Transfection
Non Transfected
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Biological Species/NCBI ID
taurine cattle
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
5.2
Measurement pX
8.28
Concomitants: Compound RN
4763661
Concomitants: Compound name
flumazenil
Concomitants: Compound role
RAD
Target Name
GABA-A receptor [human]
Target Synonyms
ionotropic gamma aminobutyric acid a receptor
Target, Subunit, Species
GABA-A receptor [human]
Target Mutant/Chimera Details
GABA-A receptor [human]:Wild
Target Species (Bioactivity)
human
Target Transfection
Non Transfected
Substance Action on Target
Inhibitor
Bioassay Category
In Vitro (Efficacy)
Biological Species/NCBI ID
human
Organs/Tissues
brain cortex
Cell Fraction
Membrane
Substance RN
702691View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
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Substance Name
flunitrazepam
Measurement Parameter
Ki
Unit
nM
Quantitative value
6.4
Measurement pX
8.19
Concomitants: Compound RN
4763661
Concomitants: Compound name
flumazenil
Concomitants: Compound role
COM
Reaxys ID 6441871 View in Reaxys
3/12 *H(1+)
Linear Structure Formula: C16H12FN3O3 Molecular Formula: C16H12FN3O3*H Molecular Weight: 314.296 Type of Substance: heterocyclic InChI Key: PPTYJKAXVCCBDU-UHFFFAOYSA-O Note:
O N O
N
N F
O
H+
Substance Label (1) Label References 50*H+
Seiler; Zimmermann; Arzneimittel-Forschung/Drug Research; vol. 33; nb. 11; (1983); p. 1519 - 1522, View in Reaxys
Druglikeness (1) 1 of 1
LogP
2.349
H Bond Donors
0
H Bond Acceptors
3
Rotatable Bonds
2
TPSA
78.49
Lipinski Number
4
Veber Number
2
Dissociation Exponent (1) 1 of 1
Dissociation Exponent (pK)
1.87
Dissociation Group
N(1+)-H
Temperature (Dissociation Exponent) [°C]
25
Solvent (Dissociation Exponent)
H2O; ethanol
Method (Dissociation Exponent)
spectrophotometric
Type (Dissociation Exponent)
a1/apparent
Comment (Dissociation Exponent)
Ratio of solvents: 98:2
Seiler; Zimmermann; Arzneimittel-Forschung/Drug Research; vol. 33; nb. 11; (1983); p. 1519 - 1522, View in Reaxys Electrochemical Behaviour (1)
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Description (Electrochemical Behaviour)
References
Electrolytic dissoci- Seiler; Zimmermann; Arzneimittel-Forschung/Drug Research; vol. 33; nb. 11; (1983); p. 1519 - 1522, View in Reaxys ation / protonation equilibrium
Reaxys ID 27883497 View in Reaxys
4/12
O N
3H
Chemical Name: [3H]-flunitrazepam Linear Structure Formula: C16H11 (3)HFN3O3 Molecular Formula: C16H12FN3O3 Molecular Weight: 315.28 InChI Key: PPTYJKAXVCCBDU-CNRUNOGKSA-N Note:
N
F N O
O
Druglikeness (1) 1 of 1
LogP
2.349
H Bond Donors
0
H Bond Acceptors
3
Rotatable Bonds
2
TPSA
78.49
Lipinski Number
4
Veber Number
2
Reaxys ID 32097874 View in Reaxys
5/12 Chemical Name: [3H]-flunitrazepam Linear Structure Formula: C16H11 (3)HFN3O3 Molecular Formula: C16H12FN3O3 Molecular Weight: 315.28 InChI Key: PPTYJKAXVCCBDU-FIRFLZKJSA-N Note:
O N 3H
O
N
N
O F
Druglikeness (1) 1 of 1
LogP
2.349
H Bond Donors
0
H Bond Acceptors
3
Rotatable Bonds
2
TPSA
78.49
Lipinski Number
4
Veber Number
2
Reaxys ID 9813319 View in Reaxys
Copyright © 2018 Reed Elsevier Properties SA. All rights reserved. Authorized use only. Reaxys® and the Reaxys® trademark are owned and protected by Reed Elsevier Properties SA and used under license.
6/12
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2H
2H
Chemical Name: flunitrazepam-d7 Linear Structure Formula: C16H5D7FN3O3 Molecular Formula: C16H12FN3O3 Molecular Weight: 320.232 Type of Substance: heterocyclic InChI Key: PPTYJKAXVCCBDU-AAYPNNLASA-N Note:
2H
O N
O
N
N F
2H
O
2H 2H
2H
Druglikeness (1) 1 of 1
LogP
2.349
H Bond Donors
0
H Bond Acceptors
3
Rotatable Bonds
2
TPSA
78.49
Lipinski Number
4
Veber Number
2
Mass Spectrometry (2) Description (Mass References Spectrometry) LCMS (Liquid chromatography mass spectrometry); Tandem mass spectrometry; QIT (quadrupole ion trap); Spectrum
Dresen, Sebastian; Kneisel, Stefan; Weinmann, Wolfgang; Zimmermann, Ralf; Auwarter, Volker; Journal of Mass Spectrometry; vol. 46; nb. 2; (2011); p. 163 - 171, View in Reaxys
spectrum; chemical ionization (CI)
Kratzsch, Carsten; Tenberken, Oliver; Peters, Frank T.; Weber, Armin A.; Kraemer, Thomas; Maurer, Hans H.; Journal of Mass Spectrometry; vol. 39; nb. 8; (2004); p. 856 - 872, View in Reaxys
Reaxys ID 32291059 View in Reaxys
7/12
O N
Chemical Name: benzodiazepine flunitrazepam Linear Structure Formula: C9H8N2*C16H12FN3O3 Molecular Formula: C9H8N2*C16H12FN3O3 Molecular Weight: 457.464 InChI Key: ZAXIUWFOWWOOLG-UHFFFAOYSA-N Note:
N N N H
F O
N O
Druglikeness (1) 1 of 1
H Bond Donors
1
H Bond Acceptors
5
Rotatable Bonds
2
TPSA
102.88
Lipinski Number
3
Veber Number
2
Reaxys ID 8389175 View in Reaxys
8/12 Chemical Name: flunitrazepam; poly(ethylene glycol) 200; mixture of Type of Substance: mixture (composition partially given)
No Structure
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Composition: Comp. Name: flunitrazepam; poly(ethylene glycol) 200 Composition: Comp. Conc.: 1 weight percent; 99 weight percent Medchem (1) 1 of 1
Bioassay Category
In Vivo (Animal models)
Bioassay Details
repeated dose toxicityNew Zealand/lop ear cross rabbit
Substance RN
8389175View in Reaxys
Substance Dose
30 μl
Substance Route of Adm.
intranasal administration
Qualitative Results
no adverse clinical signs of toxicity; no gross pathological findings related to treatment; no microscopic abnormalities in brain; flattening of olfactory epithelium
Measurement Parameter
Qualitative
Qualitative value
ND
Hjortkjaer, Rolf K.; Bechgaard, Erik; Gizurarson, Sveinbjoern; Suzdak, Carol; McDonald; Greenough; Journal of Pharmacy and Pharmacology; vol. 51; nb. 4; (1999); p. 377 - 383, View in Reaxys
Reaxys ID 8389177 View in Reaxys
9/12 Chemical Name: flunitrazepam; poly(ethylene glycol) 200; glycofurolum 75; mixture of Type of Substance: mixture (composition partially given) Composition: Comp. Name: flunitrazepam; poly(ethylene glycol) 200, 70 percent; glycofurolum 75, 30 percent; mixture of Composition: Comp. Conc.: 1 weight percent; 99 weight percent
No Structure
Medchem (1) 1 of 1
Bioassay Category
In Vivo (Animal models)
Bioassay Details
repeated dose toxicityNew Zealand/lop ear cross rabbit
Substance RN
8389177View in Reaxys
Substance Dose
30 μl
Substance Route of Adm.
intranasal administration
Qualitative Results
no adverse clinical signs of toxicity; no gross pathological findings related to treatment; no microscopic abnormalities in brain; inflammatory cells in middle part of nasal cavity; flattening and focal inflammation of olfactory epithelium
Measurement Parameter
Qualitative
Qualitative value
ND
Hjortkjaer, Rolf K.; Bechgaard, Erik; Gizurarson, Sveinbjoern; Suzdak, Carol; McDonald; Greenough; Journal of Pharmacy and Pharmacology; vol. 51; nb. 4; (1999); p. 377 - 383, View in Reaxys
Reaxys ID 8827787 View in Reaxys
10/12 Chemical Name: flunitrazepam; flumazenil; mixture of Type of Substance: mixture (composition partially given) Composition: Comp. Name: flunitrazepam; flumazenil
No Structure Medchem (1) 1 of 1
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
expression of α1 subunit protein of GABAA receptorprimary cerebellar granule cells from SpragueDawley ratsWestern blotting; optical density readings, ECL=enhanced chemiluminiscence
Substance RN
8827787View in Reaxys
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Substance Dose
1 μmol/l
Qualitative Results
no change in the expression of the GABAA receptor α1 subunit protein
Measurement Parameter
Qualitative
Qualitative value
ND
Brown, Maria J.; Bristow, David R.; British Journal of Pharmacology; vol. 118; nb. 5; (1996); p. 1103 - 1110, View in Reaxys
Reaxys ID 8827788 View in Reaxys
11/12 Chemical Name: flunitrazepam; α-aminobutyric acid; mixture of Type of Substance: mixture (composition partially given) Composition: Comp. Name: flunitrazepam; 4-amino-n-butyric acid
No Structure Medchem (1) 1 of 1
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
expression of α1 subunit protein of GABAA receptorprimary cerebellar granule cells from SpragueDawley ratsWestern blotting; optical density readings, ECL (enhanced chemiluminiscence) detection for GABAamp; receptor protein subunits
Substance RN
8827788View in Reaxys
Qualitative Results
decrease in the expression of the GABAA receptor α1 subunit protein, not significantly different from flunitrazepam (1 μM) alone, at a lower doses of flunitrazepam (1 and 10 nM) the downregulation also mirrored the effect of flunitrazepam alone
Measurement Parameter
Qualitative
Qualitative value
ND
Brown, Maria J.; Bristow, David R.; British Journal of Pharmacology; vol. 118; nb. 5; (1996); p. 1103 - 1110, View in Reaxys
Reaxys ID 8827790 View in Reaxys
12/12 Chemical Name: flunitrazepam; bicuculline; mixture of Type of Substance: mixture (composition partially given) Composition: Comp. Name: bicuculline; flunitrazepam
No Structure Medchem (1) 1 of 1
Bioassay Category
In Vitro (Efficacy)
Bioassay Details
expression of α1 subunit protein of GABAA receptorprimary cerebellar granule cells from SpragueDawley ratsWestern blotting; optical density readings, ECL (enhanced chemiluminescence) used for detection of GABAA receptor subunits proteins
Substance RN
8827790View in Reaxys
Qualitative Results
decrease in the expression of the GABAA receptor α1 subunit protein, not significantly different from the effect of flunitrazepam alone
Measurement Parameter
Qualitative
Qualitative value
ND
Brown, Maria J.; Bristow, David R.; British Journal of Pharmacology; vol. 118; nb. 5; (1996); p. 1103 - 1110, View in Reaxys
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