Brochure Becarre Natural by Gontran GAILLOT

BECARRE Natural

Nutrition October 2020

STANDARDIZED PLANT EXTRACTS

BECARRE Natural 149 rue d’Estienne d’Orves F-92140 Clamart contact@becarre-natural.com www.becarre-natural.com

OUR SUCCESS PRODUCTS

PERFORMANCE•ENERGY•FOCUS

Echinacea

News & Innovations 2019 EUROPEAN SPORTS NUTRITION AND HEALTH INGREDIENTS NEW PRODUCT INNOVATION AWARD

Nutriform Business Days 2020

Zynamite® Again 3 studies

BECARRE is Gold Sponsor

The best proven booster for “mental and physical energy”

BECARRE Natural is again Gold Sponsor of the next edition of Nutriform Business Days, which takes place from September 29th to October 1st, 2020 in the convivial setting in Saint-Raphaël, in the Var.

Zynamite® is multi-award winning patented botanical extract that increases alertness and mental activation, and boost physical energy, with regular new clinical studies and peer reviewed publications (8 studies + papers)

This symposium provides scientific and marketing solutions through a series of plenary sessions and interactive discussion groups that gives the opportunity to put any questions you may have to experts on each of the topics discussed. Networking areas helps save time and facilitates contact with partners operating within your profession, along with a series of meetings hosted by manufacturers.

Immune booster Echinacea

Again three new studies or papers on 2020, in addition to the awards: • Two ESSNA awards (Sport) : Winner ‘Best free-from product’ and ‘Runner-up Game changer’ + Frost & Sullivan recognition • Two winner nutraingredients awards plus one as finalist Cognitive health (clinical studies) • Rapid onset of mental activation • Faster reaction time • Increase performance Sport Performance (clinical studies) • Sustained energy • Increase power • Doping free

4% échinacosides, and more...

Nektium has launched their new extract of Echinacea angustifolia, standardized to 4% echinacosides... but not only...

NUTRITION

• Guaranteed 4% echinacosides, but also

for its dodecatetraenoic isobutylamide acid content - alkamide for immunomodulation • Tested by “ID testing” and DNA • Water soluble • Extract produced in Europe by Nektium Pharma (GMP certification). Echinacea angustifolia has often been overlooked in favor of E. purpurea (which is in the Pharmacopoeia), despite more than 100 published reports evaluated by the EMEA, immuno, anti-bacterial and anti-inflammatory.

Back to Gugulipid®

Alternative to Red Yeast Extract Gugulipid® from Commiphora mukul standardized to Guggulsterones Z&E (2.5% or 7.5%). Well-known but always so efficient.

Clinical studies have shown a decrease in total cholesterol, improvement of the lipid profile in the blood (decreased total cholesterol, TG, LDL, VLDL levels, increased HDL and apoprotein levels), levels of beta lipoproteins. The levels of inflammatory markers such as C- reactive protein are also reduced. Gugulipid® works through several mechanisms of action: • Inhibits cholesterol biosynthesis (by inhibiting HMG-CoA reductase cholesterol) • Improves the rate of cholesterol excretion • Promotes the rapid breakdown of cholesterol • Stimulates the thyroid • Reduction of lipid peroxides Gugulipid is the cardiovascular response for cholesterol regulation, reduction of stored fat, and inflammation. Recommended dose: 12.5 to 25 mg guggulsterones by HPLC - 3 times a day 4 - BECARRE Natural - October 2020

Sport Natural Ecdysteroids Nektium launched a new extract of Rhaponticum carthamoides standardized to min. 1% natural ecdysteroids of plant origin. Maral root (Rhaponticum carthamoides) is an herbal remedy that has long been used in alternative and traditional medicine. Often used to improve athletic performance and build muscle mass, and improve libido, maral root is mentioned to treat a variety of health issues. The root contains compounds that can influence health, including antioxidants and plant-based steroids called ecdysteroids. Maral root has adaptogenic properties similar to ginseng root (Panax ginseng), replenishing energy stores, increasing libido, sharpening focus, promoting lean muscle growth, reducing body fat, improving mood and stimulating the immune system (see page 36). 20HE (20 Hydroxy ecdysteroids) have been reported to have anabolic effects, and different preparations tested in human trials have shown diminished fat content with elevated muscle mass, increased working capacity in top athletes under intense physical activity and in bicycle exercise tests.

RedNite® Sport and now also memory RedNite® is the fresh beetroot concentrate powder standardized for 1.5%-2.75% natural nitrate, free from nitrites and synthetic nitrates - and completely water soluble with lovely red colour and pleasant taste. The extract comes from fresh beetroot processed within 24 hours from harvesting, from controlled farming conditions (to ensure no bolting of beets and higher yields) and processed at low temp to optimize to ensure best colour and actives. Previous studies were dedicated to sport : • Increased mean peak EMG amplitude and lowered motor unit firing rates, ie. improved neuromuscular efficiency of force production • Enhanced resistance to fatigue • Provided neuromuscular advantages during resistance exercise Now, new white paper showcases how RedNite® fights the signs of cognitive decline and promotes healthy aging of brain, ‘rooting for a Younger Brain’

News & Innovations GliSODin® Endometriosis / Colon cancer A new study with GliSODin® (Antioxidant Herbs Supplementation Inhibits Endometriosis Extension in Mice) demonstrated an inhibitory action on the extension of endometriotic implants.

A new study published demonstrates that GlISODin® modulates the function of vasculogenesis and angiogenesis in endometriosis tissue. This in-vivo study - which completes the list of 25 studies already published on this extract - demonstrated: • Significant decrease in the implantation surface of the endometriotic zone • Decreased expression of MDA serum and TNF-α in endometriotic tissue • Reduction in the expression of VEGF (Vascular endothelial growth factor

A second study also very recently published in Cancer Journal.

Protective role of a melon superoxide dismutase combined with gliadin (GliSODin) on the status of lipid peroxidation and antioxidant defense against azoxymethane-induced experimental colon carcinogenesis. The results showed that AOM induced ACF development and oxidative stress (GSH déplétion and lipid peroxidation) in rat colonie cells. The concomitant treatment of AOM with GliSODin significantly ameliorated the cytotoxic effects of AOM. Those results so provide in vivo evidence that GliSODin reduced the AOM-induced colon cancer in rats, through their potent antioxidant activities.

enXtra® - a 4th new study A fourth study is completing the clinically proven enXtra®, the Alpinia galanga extract to improve alertness and focus up to 5 hours with and without caffeine The new study ‘A Randomized Placebo Controlled Clinical Trial Demonstrating Safety & Efficacy of enXtra® in Healthy Adults’ has just been published. The cardiovascular safety was evaluated by assessing change in QT interval, blood pressure and heart rate. Further, other efficacy variables evaluated were change in perceived alertness and calmness, sleep disturbance and daytime sleepiness. None of the study group showed any significant change in the ECG or haemodynamic parameters as compared to baseline (p > 0.05). Post consumption, alertness and calmness scores were significantly increased in the enXtra® , and EnXtra® plus caffeine group (p < 0.001).

pTerosol

Blood sugar and cholesterol Pterocarpus is not only used for pterostilbens. pTerosol is a Pterocarpus marsupium extract, standardized to C-glycosides, the a new alternative for blood sugar and cholesterol management, besides other properties.

Nigellin® / Asthma The next anti-inflammatory Sabinsa’s Nigellin® BCS is obtained from the seeds of Nigella sativa (Black Cumin) and standardized to contain Thymoquinone, Thymohydroquinone, Hederagenin with added Rosmarinic acid.

Tinofolin®

“Capture memories forever” Tinofolin® is the extract by Sabinsa of Tinospora cordifolia, one of the extremely valued medicinal plants of Ayurveda, commonly called as “Guduchi”.

Tinofolin® was evaluated for its ability to improve visual memory, logical memory, verbal memory, attention span and concentration in a doubleblind, placebo-controlled study in 30 human volunteers aged 18 to 30 years for 21 days. Supplementation (500 mg / day) allows a significant improvement in verbal learning and memory and logical memory compared to control. Sabinsa continues its work to clarify the mechanism of action : possible increase of IL-1, and the second mechanism may be due to increased synthesis of acetylcholine.

Organic Certification BECARRE Natural offers organic certified plant extracts. In the respect of our marketing view, we still focus on standardized plant extracts, with high assay of actives, although we know that this will limit the number of available plant extracts to be promoted.

Rhodiolife®

Best Targeted Sports Nutrition Product Doping free (BSCG certified) Rhodiolife®, the proprietary extract of Rhodiola rosea convinced the judges in the category ‘Best Targeted Sports Nutrition Product +50’.

Rhodiola rosea is one of a few officially recognized adaptogens. Today, Nektium is the global leader of highest quality Rhodiola rosea, harvested in the Altai mountains of Siberia, carefully extracted in Nektium’s facilities in Spain based on many years of experience. Rhodiolife® supports a balanced lifestyle, helps baby-boomers to maintain concentration and reduces the risk of viral infection after exercise. It is side-effect and doping free as certified by BSCG. Rhodiolife® got the ESSNA award for sport nutrition.

BECARRE Natural - October 2020 - 5

NUTRITION

A study has been published on end 2018 on the anti-inflammatory and immunomodulating properties, where Nigellin® is among the most valued in anti-asthma herbal products. Nigellin had the highest effects in suppressing the release of cytokines IL-2, IL-6 and PGE2 in T-lymphocytes and also in monocytes hindering the expression on IL-2 and PGE2. These are among the most active cytokines involved in asthmatic conditions. The investigators noted a positive relationship between the TQ level in the preparations and its inhibitory potency on IL-6 and PGE2... besides existing and proved properties : glycemic control, healthy blood pressure, lipid profile, male fertility.

C-glycosidic linkages are fund to be SGLT2 inhibitors (SGLT2 catalyses the active transport of glucose into the blood stream and plays a key rôle in reabsorption of glucose in the kidney). Tests have been already conducted and results of pre-clinical study shows: • Serum glucose level : significant reduction, dose-dependant • Plasma Insulin : significant increase, dose-dependant • Total cholesterol, TG, and LDL level : significantly decreased • HDL level : significantly increased • Glycosylated hemoglobin (HbA1c) : significantly reduced, close to the normal value • Significant reduction of TNF-α and IL-6

News & Innovations Dispersible Curcumin C3

Newsroom & Last studies

A new market-ready, product formulation concept, Curcumin C3 Power Liquid , which was the Tasting Center Award Winner at Vitafoods Asia 2019 in Singapore for Best Functional Beverage.

Zynamite® : clinical trial demonstrates sustained effect on brain functions from a single dose (300mg) - August 2020

Sabinsa’s Curcumin C3 Dispersible - a standardized powder extract containing 60% Curcuminoids - effectively and efficiently resolves the solubility challenge of curcumin. Developed specifically for RTD beverages and powdered drink mixes utilizing natural excipients as a base, it can be added to water or juice, or used in chewable/ dispersible tablets. Curcumin C3 Dispersible is the GRAS-affirmed and versatile solution formulators have been looking for – and with no synthetic excipients. Curcumin C3 Dispersible is not an instant powder, as it must be firstly dissolved. But the concentrate is then easy to be used in juices, and stable.

High Lactase DigeZyme® DigeZyme® is the unique proprietary blend of specific digestive enzymes that offers much more than just aiding proper digestion and better absorption of nutrients. Sabinsa now offers DigeZyme® EC, enriched in Lactase DigeZyme : 24 000 DU (alpha amylase) / 6 000 PC (protéase neutre) / 4 000 ALU (lactase) / 200 FIP (lipase) / 1 100 CU (cellulase) DigeZyme EC: 24 000 DU (alpha amylase) / 6 000 PC (protéase neutre) / 30 000 ALU (lactase) / 200 FIP (lipase).

Starmeric™

Insoluble Turmeric Fibers

NUTRITION

Starmeric™ is the unique proprietary blend of specific digestive enzymes that offers much more than just aiding proper digestion and better absorption of nutrients. The unexplored non-polar components of turmeric like carbohydrates, proteins and fibers also have potential health benefits. Starmeric™ is a resistant starch, isolated from turmeric and having a high content of amylose. The double-helical crystalline structure of resistant starch cannot be hydrolyzed by enzymes in the small intestine and it is passed into the large intestine for fermentation by gut microflora, there it produces short-chain fatty acids. Various studies have implicated the anti-diabetic potential of resistant starch in preclinical studies

Plant extracts and SARS-COV Facing the pending pandemic, different actors have launched new studies against Sars-COV, or are compiling the results from past studies especially dedicated to this form of virus, such as :

• • • • • • •

Curcuma: prevent the entry of SARS-COV-2 through the ACE2 receptor and suppression of CYT release Nigellin®: Nigellidine and alpha-hederine were shown to bind the main structure protease of SARS-COV-2 virus by molecular docking studies GliSODin®: summary of the extensive research that Isocell/GliSODin has conducted in the field of immune health Withania somnifera : Withanone can block the COVID 19 viral replication proteine (IIT-Delhi National Institute of Avanced Industrial science & Tech) Andrographis paniculata : androphrapholides as shown to inhibit the SARS-COV-2 viral protease (in silico studies) Licorice: recent molecular docking showed that glycyrrhizin has the potential to bind to ACE2 and prevent the entry of SARS-COV-2 Probiotic: 23 trials involiving 6269 patients concluded that probiotics could decrease the incidence of respiratory infection

6 - BECARRE Natural - October 2020

Latest news or studies published

Zynamite® : “Central Nervous System activities of extract Mangifera indica L” - May 2020, in which the nootropic effect of Zynamite® is outlined based on the preclinical and pilot clinical studies that Nektium has performed. Zynamite® : Supplementation with a Mango Leaf Extract (Zynamite®) in Combination with Quercetin Attenuates Muscle Damage and Pain and Accelerates Recovery after Strenuous Damaging Exercise - February 2020 Zynamite® : US patent granted for Zynamite´s brain enhancing effects Zynamite® : New scientific article published in Nutrients : Acute Effects of a Polyphenol-Rich Leaf Extract of Mangifera indica L. (Zynamite) on Cognitive Function in Healthy Adults: A Double-Blind, Placebo-Controlled Crossover Study” C3 Complex® : Dietary curcumin significantly improves obesity-associated inflammation and diabetes in mouse models of diabesity C3 Complex® : Research on Curcumin’s Positive Effects on Microbiome C3 Complex : antineoplastic activity in mesothelioma: an in vitro and in vivo analysis C3 Complex improves cytokine level: Effects of supplementation with curcuminoids on serum adipokines in critically ill patients: a randomized double‐blind placebo‐controlled trial C3 Complex Curcuminoids plus piperine improve nonalcoholic fatty liver disease: A clinical trial enXtra® : Randomized Placebo Controlled Clinical Trial Demonstrating Safety & Efficacy of EnXtra® in Healthy Adults enXtra® : Selective enhancement of focused attention by Alpinia galanga in subjects with moderate caffeine consumption Forslean® : new patents for ForsLean®: New Scientific Discoveries Warrant Energy Balance Claims. It aids in fat conversion into heat, a novel concept called energy balance WAT / BAT (White and Brown fats) GliSODin® : Colon cancer in rats - Protective role of GliSODin on the status of lipid peroxidation and antioxidant defense against azoxymethaneinduced experimental colon carcinogenesis GliSODin®: GliSODin® Prevents Diet-Induced NASH Onset by Reducing Fat Synthesis and Improving Liver Function GliSODin®: Antioxidant Herbs Supplementation Inhibits Endometriosis Extension in Mice Lactospore®: published research confirming ability to reverse depression Symptoms in patients suffering from IBS (Irritable bowel syndrome) leads to US patent DigeZyme® : Demonstrated Efficacy in Management of Dyspeptic Symptoms with Functional Dyspepsia: Published Clinical Study Nigellin®: Comparative Immunomodulatory Activity of Nigella sativa L. Preparations on Proinflammatory Mediators: A Focus on Asthma Bettroot / RedNite®: Acute effect of a high nitrate diet on brain perfusion in older adults, + An Ergogenic Aid for Exercise and the Aging Brain Tinofolin®: Efficacy of Tinospora cordifolia on Learning and Memory in Healthy Volunteers: A Double-Blind, Randomized, Placebo Controlled Study (redit) Rhodiolife®: Rhodiolife® receives BSCG drug free certification Silbinol® : Prevention of Vascular Inflammation by Pterostilbene via Trimethylamine‐N‐Oxide. Reduction and Mechanism of Microbiota Regulation

Lemon fibers Min. 70% Total Dietary Fibers from organic lemons.

D Prebiotics, typically non-digestible fiber like the fibrous peel of the lemon, induce the growth or activity of beneficial microorganisms in the gastrointestinal tract. Dietary fiber, prebiotic, prevents and relieves constipation, normalizes bowel movements

Nutritional products Last News.................................4 Why Becarre Natural................7 Products by properties.............8

enXtra®....................................30

Key Products.........................11

Boswellin®...............................31

Plants list (Latin names)...........18 Zynamite®................................18

Pomegranate..........................32

Boosting mental and physical energy

Promond®................................20 Water soluble proteins from Almonds

Gugulipid®...............................20 Regulation of cholesterolemia

Tinofolin®.................................21 Satiety and Good Mood

Satiereal®................................22 Satiety and Good Mood

Curcumin C3 Complex®..........23 The most studied Curcuma

Rhodiolife®..............................24 Memory, Immunity & Energy

Alertness with or without caffeine

Starmeric®...............................30 Resistant starch from curcuma

Two Ways Anti-Inflammatory active All Benefits from Pomegranate

Cococin®.................................33 The Nourishment Factor

Elim’Real®...............................33 Elimination & Detoxification

Lactospore®............................34 Active probiotic Lactobacillus

BioPerine®...............................35 Ultimate Nutrients Enhancer

Rhaponticum..........................36 Powerful Natural anabolic steroids

Digezyme®...............................36 Enzymes for nutrition and sport

Bacopa....................................25

Forslean®.................................37

Bromelain / Papain.................26

Selection of previous news........ 38 Plant Decree...........................40 L’Arrêté Plante........................41 Some of regulatory decrees.....42 Our Quality Partners...............43 What is an ‘extract’ ?.............44

Revitalizes intellectual functions New enzymes in our range

Nigelllin®..................................27 New Nigella sativa

Ellagic Acid.............................27 Oral Ellagic Acid for Skin Lightening

GliSODin® ...............................28 The Effective SOD for Health & Skin

Slimming with Coleus Forslean

Process and terms

Reverse Side Personal Care Last News......................................2 Why Becarre Natural.....................3 Key Products................................4 Plants list (Latin names)....................7 New : LactoSporin® ......................8 New : Olepent®..............................9 Skin Lightening ...........................10 Comparison table sheet....................11 Boswellin®....................................11 Two Ways Anti-Inflammatory Boswellin Rich AKBBA Boswellin and Psoriasis

Anti-acne......................................12 Cosmoperine®..............................12 Enhancer of Actives and nutrients

Cococin®......................................13 The Nourishment Factor

Rhodiola®.....................................14 Stress Support and Wrinkles

Foslean®.......................................15 Forskohlin Coleus (Plectranthus) TetraPure®....................................15 What is an ‘extract’ ?..................16 Process and terms

Because industrials are more looking for reliability and know-how than an immeasurable list of products, Becarre Natural focuses on a specific origin of activities: standardized actives extracted from the nature under very strict process of production, with proven efficacy and sustainable resources. As per today, Becarre Natural represents by agreement different manufacturers, including Nektium, Sabinsa Corp., Isocell, Inoreal, … besides few others specific extracts from reputed sources selected by us. As the right note at the right time makes the music, the right product in the right formulation makes your success . ‘Becarre’ - that you may pronounce ‘be care’ - is the French word for the musical mark which cancels the alterations such sharp sign or flat signs. The English translation is ‘the natural sign’ . Gontran GAILLOT

Elodie ORLAC’H

General Manager

Sales & Dvpt Manager

+33 [0]6 86 54 83 48 +33 [0]9 56 09 05 39 gontran.gaillot@becarre-natural.com gontran.gaillot www.becarre-natural.com becarre-natural

+33 [0]7 86 08 47 93 +33 [0]9 56 09 05 39 elodie.orlach@becarre-natural.com elodie-becarre www.becarre-natural.com becarre-natural

BECARRE Natural - October 2020 - 7

NUTRITION

A SELECTION OF

PROPERTIES

Sport Especially recommended for sport, performances, muscles recovery (elderly, post-surgery) or muscles body mass or recovery. As sport induces a post-effort deficiency of the immune health, it may be interested to work on energy, power and after-sport. à Zynamite® [Mangifera indica] Nutraingredient award 2018, higher peak power output, VO2 max and oxygenation, no jitters, nervousness, agitation

à Digezyme® [Enzymes] Improves proteins intake, benefits on delayed onset muscle soreness

Mental, Alertness

à Rhodiolife® [Rhodiola rosea] Immunity post-exercise, protects muscle cells, improves endurance & recovery, adaptogenic, reduces fatigue, neurotransmission

à Rhaponticum Ecdysteroids [Rhaponticum carthamoides] Natural ecdysteroids for athletic performance and build muscle mass, adaptogen

à Fenusterols® [Trigonella foenum graecum] Use of proteins in muscles rebuilt

The effects of stress on memory include interference with a person’s capacity to encode memory and the ability to retrieve information. Age, daily stress and daily needs of attention lead us to seek support to fight anxiety, and strengthen brain alertness. Also includes mental alertness and performance.

à C3 Complex® [Curcuma longa] Anti-inflammatory, reduces muscle damage during

à Zynamite® [Mangifera indica] Nutraingredient award 2018, alertness, focus & con-

à Tinofolin® [Tinospora cordifolia] improve visual memory, logical memory, verbal

à RedNite® [Beta vulgaris] Natural nitrate, betalains & polyphenols (no nitrites nor

synthetic nitrates), performance during heavy resistance exercise, stress tolerance

à enXtra® [Alpinia galanga] Energy booster by blocking adenosine (caffeine-like without side effects), release of dopamine, increases blood flow

NUTRITION

(increase deposition of prot., increasing muscle mass), muscle power, libido

à à à à à à à à à à à à à

exercise, optimizes recovery processes, weight and cholesterol GliSODin® [Curcumis melo, Gliadine] Immune system, protects against inflammation, DNA damages and induced lactate production, anti- inflammatory cytokine BioPerine® [Piper nigrum] Ultimate nutrient enhancer, only 5mg per dose to boost by 150% to 1 000% the bio assimilation Capsaicin [Capsicum annuum] Aches & pains in muscles / joints (TRPV1), sport endurance, increases muscle protein synthesis and adrenaline secretion Cococin® [Cocos nucifera] Unique profile of nutrients from tender coconut, isotonic nutrients, blood circulation Hesperidin [Citrus sinensis] Inflammation reduction (5-LO, TNF-α, HLE), vasodilator, better blood flow, improves exercise performance (NO production) Papain [Carica papaya] Proteolytic activity, helps digest proteins and fats, diuretic and antioxidant properties Bromelain [Ananas comosus] Protein digestion, muscle repair, inflammation management Boswellin® [Boswellia serrata] Decrease of inflammation, stimulation of immunity after exercise, muscle recovery, acts on 2 pro-inflammatory enzymes Promond® [Prunus amygdalus] 50% protein from Indian almonds, 100% natural and vegan, free-flowing powder, good taste. Glu, Arg, Asp, Phe Punicic [Punica granatum] Adipose in muscles, anti-inflammatory (decrease in triacylglycerol deposits), produces a surge in testosterone, CLnA body weight Resveratrol [Polygonum cuspidatum] Antioxidant, inflammation, detoxifying Fucoxanthin [Undaria] Fat burning in white adipose tissue, thermogenin BGOV bis-glycinato oxo vanadium, organic complex. Building material of bones and teeth. Increases energy / endurance (body builders) Chrysin 97% Dihydroxyflavone, Anti-inflammatory, Anxiolytic as well

centration, faster reaction time, caffeine-like with no jitters, nervousness, agitation memory, attention span and concentration

à enXtra® [Alpinia galanga] Caffeine amplifier, focus and mental energy, alertness (sharpens alertness and focus for upto 5 hours), dopamine release

à Rhodiolife® [Rhodiola rosea] Cognitive function under stress and fatigue, antidepressant effects, good mood, adaptogenic

à Centellin ® [Centella asiatica] Memory and concentration while reducing anxiety, slows brain aging and helps regenerate neurons (cognitive impairment)

à Satiereal® [Crocus sativus] Acts on the production of neurotransmitters and hormones (serotonin, melatonin and dopamine) via the adrenal glands (SRI)

à GliSODin® [Curcumis melo L. + Gliadine] Prevents impairment memory induced by stress, promotes antioxydant defences in the brain

à Bacopin® [Bacopa monieri] Memory and neurotransmission enhancer, decrease anxiety level, increase dopamine level, neuroprotector effect

à Ashwagandha [Withania somnifera] Increase GABA A receptors activity, reduces stress and relieve anxiety, neurons protection

à Mucuna [Mucuna pruriens] Natural source of L-dopa, precursor of dopamine, action on the nervous balance (mood disorders, age-related tremors, sleep ...)

à Curcumin C3 Complex ® [Curcuma longa] Protective action (reduce inflammation in the brain), associated with Alzheimer’s disease / depression improvements

à Saberry® / Amla [Emblica officinalis] Excellent brain tonic, central nervous system à Alpha-lipoic acid Powerful antioxidant, heavy metal chelator, essential cofactor à 5-HTP [Griffonia simplicifolia] Serotonine precursor (the ‘happiness hormone’), involved in the regulation of sleep, appetite and mood

à SelenoMethionine Low plasma Se associat. selenity and cognitive decline

Anti-inflammatory, Bones, Joints, Arthritis

Weight control, Satiety

Digestion, Apetite, Transit, Colon

Memory, Attention, Anxiety, Stress

Wellness, Family health, Adaptogen

Cardiovascular health, Blood circulation

Immune System, Cold, Flue, Antibacterial

Skin, Hair, Nails

Sun protection, Tanning

Blood sugar, Cholesterol

Antioxidant, ADN

Sport, Performance, Tonic, Energy

Water soluble, Instant

Minerals, Synthetic, Intermediates

8 - BECARRE Natural - October 2020

Protection, Allergy

Inflammation Inflammation is a complex process involving a series of actions and/or reactions and a broad range of biologically active substances triggered by the body’s immunological response to tissue damage. à Boswellin® [Boswellia serrata] Inhibits the synthesis of leukotrienes, constriction of the bronchi in response to allergens, AKBBA reduced proliferation of leukemic cells

à C3 Complex® [Curcuma longa] Anti-inflammatory (COX-2 inhibitor, without side à à à à à à à à à à à à à à à à

effects unlike NSAIDs.), increases the speed of remission of cancerous cells (NF-κB) Nigellin® [Nigella sativa] Anti-inflammatory, positive effect in the context of asthmatic (stud.), anti-bacterial (Gram + germs), anti-fungal, anti-parasitic and anti-viral GliSODin® [Curcumis melo , Gliadine] Immune system, induction Th-1, INF-α IL-4 , regulate cytokines productions, DNA damages, red. allergic asthma and house dust Lactospore® [Bacillius coagulans] Improves Gut Microflora, production of Shortchain Fatty Acids, beneficial activities in gastroenteritis or irritable bowel syndrome Hesperidin [Citrus sinensis] Inhibition pain, oxidative stress, inflammatory cytokine production and TRPV1, reduction of allergy and hay fever symptoms Bromelain [Ananas comosus] Inflammation management of the gut and joints Andrographis [Andrographis paniculata] Stimulates effects on the immune system, anti-inflammatory and antipyretic properties Mango [Mangifera indica] inhibition of inflammatory modulators in the synovial tissues, hypouricemic effect (decrease serum urate levels). Punicic [Punica granatum] Anti-inflammatory (decrease in tryacylglycerol deposits), inhibit prostate cancer metastasis, pro-apoptotic Resveratrol [Polygonum cuspidatum ] Antioxidant, anti-inflammatory effects (inhibition of NFκB and AP-1), mitochondrial activ., activation of the PPARγ pathway Capsaicin [Capsicum annuum] Relieves pain by activating the TRPV1 receptor “subst. P”, triggers apoptosis of cancer cells in the stomach, pancreas and colon Ginger [Zingiber officinale] Anti-inflammatory effect (leukotrienes, prostaglandins) Eldeberry [Sambucus nigra] Boosts immune system, sinus pain, excellent sources of antioxidants, helps reduce inflammation and oxidative tissue damage Polysal helps manage inflammation and improve mechanicals strengths of bones Ursolic /Ursolate Inhibition of histamine , lipoxygenase and COX Pygeum alleviate discomfort caused by prostatic inflammation Xymenynic stimulates conversion of arachidonic into eicosanoids in the dermis

The immune system’s ability to fight infectious disease may be compromised by fatigue, aging, stress, diseases (and some related medications), and even sport. Different plant extracts exist to strengthen the immune system and assist the body to increase its defenses. à Echinacea [Echinacea angustifolia] Immunomodulator, anti-bacterial and antiinflammatory, echinacosides and dodecatetraenoic isobutylamide acid

à Rhodiolife® [Rhodiola rosea] Stimulates and protects the immune system (homeostasis), increases the natural killer cells (NK), adaptogenic

à GliSODin® [Curcumis melo, Gliadine] regulate the immune response Induction of Th1). In-vivo studies on FIV infected system and children (allergy)

à Selenomethionine / Zinc Monomethionine Bioavailable and unique composition of available selenium or Zinc

à Nigellin® [Nigella sativa] Anti-inflammatory, positive effect in the context of asth-

matic (stud.), anti-bacterial (Gram + germs), anti-fungal anti-parasitic and anti-viral

à Andrographis [Andrographis paniculata] Indian Ph. for colds, flu, fever (even cancer), powerful antioxidant, anti inflammatory and immune

à C3 Complex® [Curcuma longa] antineoplastic and immune modulating (tyrosine) à Lactospore® [Bacillius coagulans] Improves Gut Microflora, production of Short-

chain Fatty Acids, beneficial activities in gastroenteritis or irritable bowel syndrome

à Eldeberry [Sambucus nigra] Boosts immune system, sinus pain, excellent sources of antioxidants, helps reduce inflammation and oxidative tissue damage

à Hesperidin [Citrus sinensis] Inhibition pain, oxidative stress, inflammatory, reduction of allergy and hay fever symptoms (inhib. histamine release)

à Hot Sip [Adhatoda vasica, Alpinia galanga, Glycyrrhiza glabra, Piper longum] à à à à

Ayurvedic formulation cold and cough Adhatoda management of breathlessness, cold and coughs, antimicrobial Barley β-glucans [Hordeum vulgare] immunomodulator Ginger [Zingiber officinale] Anti-inflammatory (leukotrienes and prostaglandins) Fenusterol® [Trigonella foenum graecum] Expectorant, antifungal, antiparasitic

Digestion Weight, Detox Weight may be managed through different ways: 1. Decrease of the amount of certain nutrients, especially fats (less intake, different degradation and digestion/absorption), 2. Reduction of the bio transfer to the cells and muscles (education of the body), 3. Decrease of the storage of the fats, including in the liver, 4. Use of the stored fats.

à à à à à à à à à à à à à à

significant and progressive reduction of weight Forslean ® [Coleus forskohlii] Decreased Body Fat, increased Lean Body Mass by increasing the production of Adenylate cyclase enzyme - cAMP Fucoxanthin [Undaria pinnatifida] Increase fat metabolism, detoxify the digestive system, facilitating transit, helps reduce appetite Elim’Real® [Plant Formulation] Help to detoxify the body, assist renal elimination, increase urinary elimination and volume Citrin® / Garcitrin® [Garcinia cambogia] Reduction of carbohydrates in stored fat (slow down the conversion of carbohydrates), increases the use of fat Bromelain [Ananas comosus] Proteolytic activity, helps to better digest., reduces gastric acidity, reduces swelling and flatulence, reduces cellulite (anti-oedematous) Papain [Carica papaya] Proteolytic activity, helps digest proteins and fats, diuretic and antioxidant properties Lactospore® [Bacillus coagulans] Improves gut microflora, production of ShortChain Fatty Acids, beneficial activities in gastroenteritis or irritable bowel syndrome Fabenol® [Phaseolus vulgaris] Alpha-amylase inhibitor, “starch bloker”, benefits in the maintenance of healthy blood sugar levels and optimal body composition Gymnema GS4+® [Gymnema sylvestre] Promotes bodies production of insulin, also works on weight management (“sugar blocking” property), sweetness inhibitors Lemon fiber [Citrus limon] Dietary fiber, prebiotic, prevents and relieves constipation, normalizes bowel movements Curcumin C3 Complex® [Curcuma longa] Allows to make fat soluble for digestion and elimination of fat cells, helps cells with glucose absorption, remove gallstones Nigellin® [Nigella sativa] Draining effect helps in the elimination of toxins in the intestines and lungs, stimulates the digestive and intestinal systems Saberry® / Amla [Emblica officinalis] Stimulates gastric juices and also support detoxification, possess antisecretory, antiulcer, and cytoprotective properties Capsaicin [Capsicum annum] Stimulates metabolism, increases fat burning, decreases appetite (inc. of GLP-1), anti-inflammatory on H. pylori-induced gastritis

à Digezyme® [Multi-enzyme complex] Multi enzyme complex, studied enzymatic profile: α-Amylase, Protease, Lipase, Cellulase, Lactase

à Lactospore® [Bacillus coagulans] Improves gut microflora, production of Short-

Chain Fatty Acids, beneficial activities in gastroenteritis or irritable bowel syndrome

à Bromelain [Ananas comosus] Proteolytic activity, helps to better digest., reduces

gastric acidity, reduces swelling and flatulence, reduces cellulite (anti-oedematous)

à Papain [Carica papaya] Proteolytic activity, helps digest proteins and fats, diuretic and antioxidant properties

à Licorice DGL ® [Glycyrrhiza glabra] Decreases the acidity of the stomach, calms the irritation of the lining of the stomach

à Elim’Real® [Formulation] Help to detoxify the body, assist renal elimination, increase urinary elimination and volume

à Starmeric™ [Curcuma longa] Resistant starch from turmeric roots à Berberine [Berberis aristata] Indolent ulcers, stomach concerns (supports the healthy bacteria)

à Triphala [Terminalia belerica, Terminalia chebula & Emblica officinalis] support digestion

à Pomegranate [Punica granatum] useful against flatulence, difficult digestion, diarrhea, bloating

à Nigellin® [Nigella sativa] Draining effect helps in the elimination of toxins in the intestines and lungs, stimulates the digestive/intestinal systems

à Saberry® / Amla [Emblica officinalis] Improve the stimulation of gastric juices and also support detoxification, antisecretory, antiulcer, and cytoprotective properties

à Ginger [Zingiber officinale] Antiemetic effects by reducing the movements of the stomach

à Curcumin C3 Complex® [Curcuma longa] Allows to make fat soluble, digestion

and elimination of fat cells, helps cells with glucose absorption, remove gallstones

à Capsaicin [Capsicum annuum] Stimulates metabolism, fat burning, decreases appetite (GLP-1), anti-inflammatory effects on H. pylori-induced gastritis

à à à à

Fenufiber and galactomannans stimulates digestion Acticissus protection against ulcers Citrin slow conversion of sugars Asparagus gastric secretion / ulcers BECARRE Natural - October 2020 - 9

NUTRITION

à Satiereal ® [Crocus sativus] Moderate appetite, reduce compulsive snacking,

Digestion may mean different actions, with possible combinations : Maximize the uptake of nutrients, Improvement of the digestive wellness, as -well as the welfare of the intestinal tract, Allow people eating less or just make the most of their diet, Prevent heartburn, ulcers, fatigue, nauseas and irritations related to weak digestion + all adaptogenic products :

Cardiovascular Some products have been especially studied for their action on Metabolic Syndrome, besides those with properties against oxidative stress, cholesterol, blood sugar level and globally over-weight. à GliSODin® [Curcumis melo , Gliadine] Reduces risk factor of cardiovascular disease, decrease carotid intima-media thickness

à Gugulipid® [Commiphora mukul] Inhibits cholesterol biosynthesis, Improves the à à à à à à à à à à à

rate of cholesterol excretion and rapid breakdown. Reduction of stored fat Venocin® [Aesculus hippocastanu] Helps maintain healthy blood circulation and strengthen the capillaries and veins, standardized in escin Berberine [Berberis aristata] Lower cholesterol similar to statins, Reduces blood sugar levels in Type II, lowers blood pressure by acting on AMPK Curcumin C3 Complex® [Curcuma longa] reduces serum concentration of LDL-C, non-HDL-C, total cholesterol, TG and Lp(a), helps cells with glucose absorption pTerosol® [Pterocarpus marsupium] cholesterold and blood sugar management Centellin® [Centella asiatica] Triterpenic fraction has beneficial effects in people with venous insufficiency and varicose veins, venotonic activity Hesperidin [Citrus sinensis] Vasodilator action, improves the integrity of the blood vessels Gymnema GS4+® [Gymnema sylvestre] hypoglycemic effect, hypolipemic effect (improves lipids metabolism) Sabbery® [Emblica officinalis] Hypocholesterolemia (reduction of LDLc and TH, with high increase in HDL), improves glucose metabolism in Type II Resveratrol [Polygonum cuspidatum] Permits reduction of serum concentration of LDL, anti-oxidant effect Fenumannans® [Trigonella foenum graecum] improves the blood profile: reduction of LDL cholesterol and triglycerides Ellagic acid [Punica granatum] Decreases the oxidation of LDLc, antioxidant effect, prevents the formation of atherosclerotic plaque (act on paraoxonase (PON 1)

Prostate, Oncology, Tumor This is for information only based on available studies and literature, and has no purpose to suggest claim as we here speak of diseases. à Boswellin® [Boswellia serrata] AKBBA reduced vitability and proliferation of leukemic cells, Inhibits DNA synthesis and cell growth of HL-60 and topoisimerse I and II

à C3 Complex® [Curcuma longa] anti-inflammatory, increases the speed of remission à à

NUTRITION

à à à

of cancerous cells (downregulation of NF-kB, AP1, JNK,) GliSODin® [Curcumis melo, Gliadine] strong action on immune system, recent study on impact on radiation-induced Fibrosis (colon cancer) Fenusterol® [Trigonella foenum graecum] protects against diseases related to premature aging of cells such as breast, prostate, colon and gallbladder cancer Amla [Emblica officinalis] Studied on growth of cancer cells in mammary, pancreatic or prostate tumors (cell lines A549, HepG2, HeLa, MDA-MB-231 etc...) Punicic [Punica granatum] inhibitory effects on prostate cancer growth, angiogenesis and metastasis, pro-apoptotic Punicalagin [Punica granatum] action on PC3 capability to arrest proliferation and stimulate apoptosis Capsaicin [Capsicum annuum] triggers apoptosis of cancer cells in the stomach, pancreas and colon Ashwagandha [Withania Somnifera] Adaptogenic, delay the process of cellular aging, decrease the activity of acetylcholinesterase and slow down turmor cells dvlpt Centellin® [Centella asiatica] Slows the development of solid and ascites tumors, induce apoptosis in different cancer cell lines (esp. MCF-7, breast)

Medicinal Plants and Cancer Chemoprevention Also... Saw Palmetto, Pygeum, Rhodiola, Piperine, DIM, Nigellin (liver detox)

Nutricosmetics The skin is the single largest organ of the body. Different factors can cause skin problems : heat, cold, sun… Below is a very short list of available actives, without here speaking of the skin lightener / whitener, or only topical. à GliSODin® THE most dedicated oral protection of the skin, Increase of the MED à à à à à à à à

(resistance to UV induced erythema or redness), improves tanning speed and quality, drastically decreases the oxidative stress (more than 20 published studies) Saberry® from Amla [Emblica officinalis], rejuvenative properties, UV protection, Melanogenesis inhibition (In vitro study), Antioxidant activity Lycopene [Lycopersicon esculentum] antioxidant, protect the skin from UV rays Ellagic Acid Inhibits melanin formation (In vit. st.), hyperpigmentation (Clin. st.) L-SelenoMethionine bioavailable selenium and methionine, important in the synthesis of keratin and collagen for the structure of skin, nails and hair Hesperidin [Citrus sinensis] inhibits the development of cutaneous edema, neutrophil recruitment, matrix metalloproteinase activity, powerful antioxidant Rosemary Extract and its conversion into intermediates like rosmanol and galdosol both of which are free radical scavengers Silbinol® from dried heart wood of Pterocarpus marsupium for pterostilben, potent suppressor of advanced glycation endproducts (AGEs) (In vitro) Punicic from pomegranate, heal, protect, and moisturize dry, cracked, mature, and irritated skin Brown seaweeds, Fucoxanthin Inhibition of hyperplasia and induce apoptosis

à And all others showing great antioxidant properties : FruitOx®, Curcuma, etc...

Blood Sugar Malfunction of the regulation of the blood sugar levels can have various causes (insulin secretion or insulin response, also related to the ingestion of sugar.). Types of diabetes are rated «I» (IDDM: insulin by the pancreas, insulin dependent), «II» (NIDDM, resistance to insulin / non-insulin dependent) or «gestational». Other products may be recommended to manage the consequences (cardiovascular, neuropathy, nephropathy, ...). à pTerosol® [Pterocarpus marsupium] C-glycosides for cholesterold and blood sugar management

à Gymnema GS4+® [Gymnema sylvestre] hypoglycemic effect by promoting the

bodies production of insulin, clinical studies on Type I and II, hypolipemic effects

à Fabenol® [Phaseolus vulgaris] Alpha-amylase inhibitory that blocks the digestion of dietary starch, permits maintenance of healthy blood sugar levels

à Starmeric [Curcuma longa] Resistant starch from turmeric roots à Berberine [Berberis aristata] blood sugar levels in people with Type II diabetes (inhibition of α-glucosidase), and lowering blood pressure by acting on AMPK

à Lactospore® [Bacillus coagulans] Production of Short-Chain Fatty Acids, used for

lipogenesis and gluconeogenesis, insulin-glucose through glucagon-like peptide 1

à Fenumannans® [Trigonella foenum graecum] Fibers or Glucomannans - Regulation of blood glucose levels in type 2 diabetes, improves blood profile

à Nigellin® [Nigella sativa] Can reduce blood sugar levels and insulin resistance à Curcumin C3 Complex® [Curcuma longa] Effect of curcuminoids on Metabolic Syndrome, helps cells with glucose absorption

à Ginger [Zingiber officinale] Decreases fasting blood glucose and hemoglobinglycated levels, and improves insulin resistance

à Ashwagandha [Withania Somnifera] Adaptogenic, reduces blood sugar levels à Sabbery® [Emblica officinalis] Hypocholesterolemia, improves glucose metabolism in Type II , reduces biomarkers of oxidative stess and inflammation

à Momordicin sugar level / insulin / Mango sugar (αα-glucosidase) / Silbinol insulin and sugar / Salaretin insulin & sugar / Tulsi sugar level

Minerals & Others You may find the full list by properties or by sectors on the website. Just a quick look at some activities, not mentioned in others. à BioPerine: the ultimate nutrient enhancer, only 5mg per dose to boost by 150% to 1 000% the bio assimilation

à Minerals: not the common: Zinc, Selenium, Molybdenum, Chromium, Vanadium, ... à Respiratory support: Andrographis, Centella, Elderberry, Adhatoda, Coleus, ...

• OIls : Coleus Forskholii Oil, Rosemarinic acid, Curcuma Turmeric Oil, Vitex Oil (Artemetin), Mustard seed, Policosanol, ....

10 - BECARRE Natural - October 2020

Adaptogen Adaptogenic herbs demonstrate a nonspecific enhancement of the body’s ability to resist a stressor. Only the most common here: à à à à à

Rhodiola Rhaponticum (Rhaponticum carthamoides) Ashwagandha Asparagus (Shatavari) Amla (Emblica officinalis or Indian gooseberry)

KEY PRODUCTS Anti-inflammatory, Bones, Joints, Arthritis

Weight control, Satiety

Digestion, Apetite, Transit, Colon

Memory, Attention, Anxiety, Stress

Wellness, Family health, Adaptogen

Cardiovascular health, Blood circulation

Immune System, Cold, Flue, Antibacterial

Skin, Hair, Nails

Sun protection, Tanning

Blood sugar, Cholesterol

Antioxidant, ADN

Sport, Performance, Tonic, Energy

Water soluble, Instant

Minerals, Synthetic, Intermediates

Zynamite®

18-19

Mental alertness, Performance, Power, Weight, Caffeine replacement Nutraingredient Award Vitafoods 2018 & 2019, ESSNA Award 2019 Origin: Nektium (Spain) Source: Mangifera indica, Leaves Bio-active ingredients: Mangiferine Dose: 2 mg to replace 1mg caffeine, to up to 2 x 300mg

Echinacea angustifolia Common cold, Immune system Origin: Nektium (Spain) Source: Echinacea angustifolia – root (European origin) Bio-active ingredients: Echinacosides ≥ 4% (HPLC), Dodecatetraenoic acid isobutylamides(≥ 0.1 %)

Zynamite is the new product from Nektium, with proven efficacy on mental alertness and performance, as well as for sport, without jitters, nervousness, agitation usually linked to caffeine (2 mg to replace 1mg) Mental : improves the alertness, focus and concentration - with faster reaction time, and with no jitters, nervousness, agitation. It activates brain waves and increases Long Term Potentiation in a remarkably similar pattern to caffeine, but without any of the know side-effects Sport : higher peak power output, VO2 max and oxygenation, without change in blood pressure or hearth rate. Other beneficial effects of Mangifera indica: Preclinical studies suggest that it may prevent tumor necrosis factor, with expression of some mediators and enzymes involved in inflammation, pain, oxidative stress and synaptic plasticity. Also reported: Weight management, antidiabetic, anti-infective, antimicrobial and antioxidant. Inhibition of 5-α reductase. Promote endothelial cell migration

Traditionally used to support the immune system and strengthen the body’s resistance against bacterial and viral diseases (historically by Native Americans to treat infections of the upper respiratory tract and urinary system). Publications have reported Echinacea stimulates the specific immune response, strengthens the immune system and reduces pain related inflammatory processes. E. angustifolia has often been overlooked in favor of E. purpurea, in spite over 100 published reports assessed by the EMA for E. angustifolia - non-clinical and clinical safety and efficacy. This high quality extract produced by Nektium Pharma meets many criteria: Soluble in water 100% made in EU ingredient Standardized in Echinacosides and Dodecatetraenoic isob. acid Accredited European raw material supplier Tested by “ID testing” and ADN GMP certified factory production in the Canary Islands

pTerosol™

Gugulipid®

Cholesterol, blood sugar “the miracle cure for diabetes” in Ayurveda literature Origin: India Source: Pterocarpus marsupium Bio-active ingredients: 5% C-glycosides (as Pterocarposide and Sabioside), Tannins

Cholesterol, Inflammation Origin: India Source: Commiphora mukul Bio-active ingredients: 2.5% / 7.5% Guggulsterones E & Z

DigeZyme®

Ultimate multi-enzyme complex for Digestion and Nutrients Origin: Sabinsa (India) Dose : 50 mg per dose, before eating

A multi-enzyme complex consisting of Amylase (starch digesting), Lipase (fat digesting enzyme), Cellulase, Protease (protein digesting enzyme) and an additional component is the enzyme lactase, which is useful for people suffering from lactose intolerance (positive opinion from EFSA). DigeZyme helps in the digestion of carbohydrates, proteins and fats, which are poorly assimilated by our bodies in cases of unbalanced diets and impaired pancreatic function.

Gugulipid® is an historical product launched years ago by Sabinsa, but always so efficient that it’s now more and more suggested as the alternative to red yeast extract and its limitations. Clinical studies have shown improvement in the lipid profile in the blood, lipoprotein levels and markers of inflammation. Sabinsa also highlighted the mechanism of action of guggulsteronnes to improve the elimination of cholesterol and the inhibition of its biosynthesis. Improves thyroid functions (increase of peroxidase and proteolytic activities).

Rhodiolife®

24-25

Immunity, Adaptogen,Memory and attention Energy, Aids in sports performance (Drug free BSCG) No excipient ESSNA Award 2019 Origin: Nektium (Spain) Source: Rhodiola rosea – roots from Altai mountains Bio-active ingredients: rosavins 3-15%, salidroside 1-7%, spray dried or freeze-dried (comparison study), water or low ethanol extract. Highly selected profile from the roots to the end-extract. Dose : 2 to 3 x 200mg based on 3%. Some studies starting from 50mg.

Rhodiola rosea has been considered a potent adaptogen for decades. Adaptogenic compounds help the body counteract the effects of stress, they are mostly not toxic to the body and their mechanism of action is non-specific meaning that various organs and systems are impacted upon to restore the body´s balance. Studies available and a complete monograph published by Nektium describes the origin and the efficacy. Clinical trials with Rhodiola rosea extracts have shown significant improvements in fatigue, exercise capacity and mental performance under stressful conditions. It also reduce the level of C-reactive protein and creatinine kinase in the blood, and assists the immune system and improve memory and attention.

BECARRE Natural - October 2020 - 11

NUTRITION

pTerosol™ is a new alternative for blood sugar and cholesterol management, besides other properties, from Pterocarpus marsupium. pTerosol™ has put in evidence the efficacy of C-glycoside. C-glycosidic linkages are fund to be SGLT2 inhibitors (SGLT2 catalyses the active transport of glucose into the blood stream and plays a key rôle in reabsorption of glucose in the kidney). Tests have been already conducted and results of pre-clinical study shows: • Serum glucose level : significant reduction, dose-dependant. After 12 weeks, 69% patients showed significant control of blood glucose (fasting and postprandial level) • Plasma Insulin : significant increase, dose-dependant. 36 week study found that P. marsupium extract gives similar results to tolbutamide (a standard antidiabetic agent), without the known side effects • Total cholesterol, TG, and LDL level : significantly decreased • HDL level : significantly increased • Glycosylated hemoglobin (HbA1c) : significantly reduced, close to the normal value • Significant reduction of TNF-α and IL-6

• • • • • •

enXtra®

Rhaponticum (ecdysteroids)

Energy, Performance, Focus - Caffeine Amplifier Nutraingredient Award Vitafoods 2019 Origin: India Source: Alpinia galanga Bio-active ingredients: Polyphenols, Flavonoids, Tannins Dose: 300 mg

enXtra® can either replace caffeine, providing a non-caffeinated mental energy boost or it can accompany caffeine to Amplify Caffeine benefits and reduce caffeine induced crash. enXtra® is fully water soluble with mild taste and lovely tea color. Extracted from rhizomes of Alpinia galanga, grown in pollution free hilly terrain, is a DNA-authenticated. Application : enXtra® is ideal functional ingredient for energy drinks (with or without caffeine), ready to drink beverages of all types, sports nutrition and workout formulas, energy bars, snacks, gummies, juices, chewing gum and stick packs. Studies : enXtra® is supported by human clinical trials. In a 53-person, doubleblind, double-dummy human clinical trial, enXtra® was shown to improve mental alertness and acuity for up to 5 hours without increasing heart rate or blood pressure.

BioPerine®

Ultimate Nutrient Enhancer Easy to use, well-known and efficient booster Origin: Sabinsa (India) Source: Piper nigrum L (black pepper) Bio-active ingredients: piperine The dose is 5mg per intake

Very well documented and patented (“use of piperine as a gastrointestinal absorption enhancer”), the BioPerine® increases the bio availability / bio adsorption of most of liposoluble compounds. Many studies are available especially versus curcuminoides (> 10 times), beta caroten (+160%) and thereof other carotenoids, CoQ10 (+150%), Resveratrol (> 200% and > 10 times in serum), liposoluble vitamins (B6: +250%), Selenium (+145%), etc… Although some competitors try to discredit BioPerine® , it’s today the most effective, the cheapest and most safe way to enhance bioavailability. It’s the only piperine confirled non novel foods (2011) and not subject to EFSA since it’s a « nutrient enhancer ». The process uses exclusively ethanol (also for the oleoresin production).

NUTRITION

Curcumin C3® Complex

Antioxidant, Preservative, Antimicrobial, Immune system, Anti-inflammatory, Metabolic Syndrome The original, and most studied Curcuma Origin: Sabinsa (India) Source: Curcuma longa—roots Bio-active ingredients: FREE Curcuminoids (3 fractions) - and not the inactive metabolites as sulphate or glucoruronides Usual dose is 3 x 400mg (alone) to 2 x 50mg, with possibly 5mg BioPerine. Standard, Direct Compression or even Beadlets grades The grade with its own safety data, and more than 100 published studies, mostly available on curcuminoids.com/index.php/benefits/clinicalevidence Sabinsa is the world leading manufacturer of Curcuma extract 95%, processing in their dedicated plant (2 continuous batch extractors with food solvents), processing roots from their contracted fields. They control the 3 curcuminoids to prevent adulteration with synthetic curcuminoides (a 95% extract includes curcuminoid (70-75%), demethoxy (15-25%) and bis-demethocy (2.5-6.5%). Curcumin C3® Complex contains free curcuminoids only, and no inactive metabolites (glucoronide for instance), because all form of curcuminoids are not equal. Some forms can be bioavailable, but unfortunatley inactive. BioPerine - and its history of more than 20 years of use without any safety alert improves the availability without changing the form. Another significant study on Curcumin C3® Complex (in combination with BioPerine®) has been published on end of 2014, showing the significantly greater effect of Curcuminoids in reducing the serum concentrations of LDL-C, non-HDL-C, total cholesterol, triglycerides and Lp(a), when serum HDL-C concentration was elevated significantly. Serum sdLDL levels were comparable It’s probably the most studied curcuma extract (hundreds studies incl. colorectal cancer prevention), and usually referred as the highest quality (and the lowest oxalic content, causing kidney stones, heavy metals).

12 - BECARRE Natural - October 2020

Natural vegetal anabolic ecdysteroids, Muscle mass, Immunity Origin: Nektium (Spain) Source: Rhaponticum carthamoides – roots Bio-active ingredients: 1% Ecdysteroids

It brings ecdysteroids which have been reported to have anabolic effects in different animal species (Syrov et al.). Different preparations tested in human trials have shown diminished fat content with elevated muscle mass in track and field athletes (A comparative study of the anabolic action of Ecdysten, Leveton and Prime Plus, preparations of plant origin), increased working capacity in top athletes under intense physical activity and in bicycle ergometry exercise tests with gradually increasing physical loads (Azizov et al). Rhaponticum carthamoides does not bind to androgen receptors, this alternate mechanism avoids the unwanted side effects of steroids while reaping many of the positive benefits.

Satiereal®

Innovative, patented, natural and safe appetite suppressor Weight losses and good mood Origin: Inoreal (France) Source: Crocus sativus L. stigmas, Mediterranean region Bio-active ingredients: Specific and studied unique profile of crocin, picrocrocin and safranal - it’s more than a saffron extract The daily dose is 176.5mg (2 X 88,25mg/d) used in tablets, capsules, beverages and instant drinks, confectioneries, …

Satiereal® is the patented clinically proven satiety ingredient from saffron. The satiated feeling induced by Satiereal® encourages weight loss while eliminating frustration. This product has a unique mechanism that not only creates satiety but helps avoid snacking and compulsive eating behaviors, which in turn leads to reduction in weight and inches. Satiereal® is a Serotonin Reuptake Inhibitor (SRI), working so on the general good mood. New studies regularly published.

Fenusterols®/Fenufibers® Digestion, Blood Sugar Origin: Sabinsa (India) Source: Trigonella foenum graecum Dose : 25g (Fenufibers), 1-5g (Fenumannans), 100-200mg (Fenusterols)

Sabinsa extracts different products from fenugreek : • Fenusterols steroidal saponin (men & women, muscles,...) • Fenumannans Galactomannan (blood sugar) • Fenufibers (digestion, serum cholesterol, TGL, VLDL) : nutritive & restorative properties, stimulates digestive process

Lactospore® / Lactowize®

Health of the intestinal tract, Probiotic Origin: Sabinsa (India) Source: Lactobacillus sporogenous (also called coagulans), in a 100% dedicated plant. Formulation with fibers (Lactowize®) Bio-active ingredients: 6 to 15 billion spores Dose : 100 to 200 Millions organisms per doses, 2-3 times a day, in tablets, capsules, chewing tablets, etc...

Digestion, cholesterol management, health of the intestinal tract, … and can be formulated with prebiotic easily, or choose our Lactowize® (15 Bll spores and >35% galacto-mannans) After ingestion (medium for awakening), the spores revive and produce acid L (+) Lactic, with the positive action on other micro-organisms. Virtually 100% of spores are revived, one reason for the choice of Bacillus coagulans, and specific drying method. Spores will not grow during their action, and disappear by natural ways after working, hence the need for regular intake. Extra protection : Sabinsa provides a grade ‘EC’ Billions of Lactspore 15 (‘EC’ for Enteric Coated), a protected form for more targeted action. It is used in dietary supplements in dairy products (studies currently being finalized), bakery products, or in tablets or vaginal ovules.

Nigellin®

Blood Sugar, Liver, gut health, anti-inflammatory, profertility Origin: Sabinsa (India) Source: Nigella sativa – Black cumin seed extract Bio-active ingredients: Thymoquinone (TQ)

Nigellin® from Sabinsa, is a black cumin seed extract obtained by supercritical fluid extraction, standardized in thymoquinone. Traditionally used in cases of nasal congestion, immune deficiency (and anticancer action by Cancer Immuno-Biology Laboratory of Hilton) of the liver, or still headache / throat / tooth. Sabinsa studied various activities including antimicrobial and antifungal activity (reported synergy with various antibiotics) as well as anti parasitism, activity on the respiratory system (would act as a natural anti-elastase), or anti -Inflammatory and analgesic. Black cumin is traditionally used in patients with diabetes. Rachid H and col. (2005) have shown that cumin seed has an effect on insulin secretion. Another activity reported is the help with digestion: Carminative activities facilitate digestion and relieve gas problems in the stomach and intestines. It helps peristalsis and therefore elimination. New Sabinsa study : Anti-inflammatory and immunomodulating properties are among the most valued in anti-asthma herbal products. Nigellin had the highest effects in suppressing the release of cytokines IL-2, IL-6 and PGE2 in T-lymphocytes and also in monocytes hindering the expression on IL-2 and PGE2. These are among the most active cytokines involved in asthmatic conditions. The investigators noted a positive relationship between the TQ level in the preparations and its inhibitory potency on IL-6 and PGE2. Nigellin is available as oil (Nigellin BCS - Amber), and defatted black and white powders (Nigellin BCS - Onyx and Nigellin BCS - Pearl, respectively).

Venocin® Horse Chestnut Anti-inflammatory, Blood circulation Origin: Sabinsa (India) Source: Aesculus Hippocastanum (seeds) Dose: 250mg Bio-active ingredients: Escin (or aescin)

Venocin features this unique compound known as aescin or escin, a mixture of saponins that is known to help maintain healthy blood circulation and strengthen the capillaries and veins. Horse chestnut extract is clinically proven to be beneficial to people suffering from varicose veins, spider veins, hemorrhoids and related circulatory problems or “chronic venous insufficiency” (CVI).

Bromelain

RedNite® Sport Energy, Performance, Antioxidant, Brain-health Origin: India Source: Beta vulgaris L. Bio-active ingredients: Nitrate, Betalain Dose: 500 mg / dose Powder standardized for 1.5%-2.75% natural nitrate, free from nitrites and synthetic nitrates - and completely water soluble with lovely red colour and pleasant taste. RedNite® comes from fresh beetroot processed within 24 hours from harvesting, from controlled farming conditions (to ensure no bolting of beets and higher yields) and processed at low temp to optimize to ensure best colour and actives. Previous studies were dedicated to sport : • Increased mean peak EMG amplitude and lowered motor unit firing rates, ie. improved neuromuscular efficiency of force production • Enhanced resistance to fatigue • Provided neuromuscular advantages during resistance exercise Up to 500mg to more serving size for RedNite® . Dose is lower for Chewable product like sports bars, lozenges, functional foods etc. Brain health: Now, new white paper showcases how RedNite® fights the signs of Cognitive decline and promotes healthy aging of brain, ‘rooting for a younger brain’.

Tinofolin®

Memory and Focus Origin: India Source: Tinospora cordifolia Bio-active ingredients: Bitter principles Dose: 3 x 100 mg to 500 mg

Tinofolin® was evaluated for its ability to improve visual memory, logical memory, verbal memory, attention span and concentration in a double-blind, placebo-controlled study in 30 human volunteers aged 18 to 30 years for 21 days. Supplementation (500 mg / day) allows a significant improvement in verbal learning and memory and logical memory compared to control.

Fabenol®

Bromelain is a cysteine protease isolated from pineapple stem, Ananas comosus. Bromelain is a mixture of different thiol endopeptidases and other components like phosphatase, glucosidase, peroxidase, cellulase, escharase, and several protease inhibitors. In vitro and in vivo studies demonstrate that bromelain exhibits various fibrinolytic, antiedematous, antithrombotic, and anti-inflammatory activities. When given to treat pain, it must be administered away from food, because it will act as a protein digestive enzyme if consumed with food.

Fabenol® is an alpha-amylase inhibitory naturally extract obtained from Phaseolus vulgaris (common bean, kidney bean) that blocks the digestion of dietary starch, thereby offering potential benefits in the maintenance of healthy blood sugar levels and optimal body composition. The seeds of Phaseolus vulgaris contain an inhibitor of alpha-amylase (two glycosylated protomers of 30 kDa, which are cleaved into two polypeptides of 16 and 14 kDa, respectively), identified in the protein fraction of the bean. This protein binds with the active sites of alpha- amylase and prevents the starch metabolizing activity. Hence it is also called as «Starch Blocker».

Papain

Starmeric™

Digestive aid, Antimicrobial, Inflammation Origin: India Source: Carica papaya Bio-activity: 1,5 to 3,5 FIP / mg Units: 1 FIP ≈ 10 to 12 000 USP ≈ 160-180 TU / 100 TU ≈ 6 000 USP ≈ 0,6 FIP

Papain, a papaya fruit latex extract, is a cysteine protease that catalyzes the cleavage of peptide bonds using a catalytic triad involving a deprotonated cysteine residue. Papain is well known for improving digestion and treating infections, diarrhea, and allergies. It has also been used, with other protease, to reduce pain, inflammation, and swelling. Also being studied for use in cancer and other diseases.

Blood Sugar, Antioxidant, Anti-inflammatory Origin: Sabinsa (India) Source: Curcuma longa Bio-active ingredients: 40% Turmeric Resistant Starch Dose : 500mg twice a day

Starmeric™ is a resistant starch, isolated from turmeric, lowering the postprandial glucose level and can also improve the insulin sensitivity Also anti-rheumatoid activity against Mycobacterium tuberculosis induced adjuvant arthritis, and can improve serum lipid profile, colon health and gut microbiome.

BECARRE Natural - October 2020 - 13

NUTRITION

Inflammation, Allergy, Digestive aid Origin: India Source: Ananas comosus - Stem Bio-activity: 1 200 to 5 000 GDU / g ≈ 2,4 to 10 FIP / mg Units: 1 FIP/mg ≈ 500 GDU/g Dose: 200 mg to 2g

Amylase inhibitory activity Origin: Sabinsa (India) Source: Phaseolus vulgaris Bio-active ingredients: 8 000 units/g (Fabenol) or 20 000 units / g (Fabenol Max) Dose: usually 1 000 - 5 000 units per dose, depending upon the starch content of the meal.

Boswellin®

GliSODin®

The anti-inflammatory power of AKBBA Origin: Sabinsa (India) Source: Boswellia Serrata—Gum resin exudate from the tree Bio-active ingredients: Acetyl-11-keto-b-boswellic acid, b-boswellic acids, boswellic acids Dose is 75 to 200 mg once to twice a day depending on grades

First orally proven effective SOD : Sun protection, Antiaging, Metabolic syndrome, Oxidative stress Origin: Isocell (France) Source: Curcumis melo L., Gliadine Bio-active ingredients: SOD, Gliadine

Boswellin is the very powerful anti-inflammatory extract of Boswellia serrata gum, standardized for its content in not only boswellic acid but mainly AKBBA, acting on two path ways : 5-lipoxygenase and HLE (Human Leukocyte elastase). Topical or oral use (anti-inflammatory of course, but also anti-wrinkle, anti-aging and skin firmness). Grades are not titled as «total boswellic acid» - which means nothing in either since all acids are included - but specifically each beta-boswellic and AKBBA (Super grade is even up to 30% AKBBA). Oral Grades (HBD, Super, Forte, OS) and topical grade (CG).

ElimReal®

Water elimination and detox, weight management Origin: Inoreal (France) Source: Plant formulation Dose : 805 mg per day

Elim’Real® is a patented extract of different plants with complementary activities in detoxifying the body and assisting the renal elimination , which has clinically demonstrated its effectiveness (study on 95 women over 28 days, double-blind against placebo) and allows healthy allegations thanks to some of its components. It’s a plant formulation from Carvi’s seeds (Carum carvi), Meadowsweet Flower buds (Spirea ulmaria), Guarana’s seeds (Paullinia cupana), Goldenrod’s plant (Solidago virgaurea), Fennel’s fruits (Foeniculum vulgare) and Dandelion’s leaves (Taraxacum dens leonis). ElimReal® is covered by a patent for slimming applications, and promotes renal elimination functions (excretory function of the kidneys) / urinary elimination /urinary volumes, so works on detoxification and slimming. ElimReal® is easy soluble in water, and may be used in any form of dietary supplements.

NUTRITION

Fucoxanthin Support healthy body weight / Liver, Antioxidant Origin: Nektium (Spain) Source: Wakame Bio-active ingredients: Fucoxanthin by UPLC, Fatty acids (PUFA), Fucoxanthin 10% UV powder Dose : 15 mg fucoxanthin

The scavenging activity of fucoxanthin is almost 8 times higher than that by fucoxanthinol, and more than 13 times higher than that by alpha-tocopherol. Addition of Fucoxanthin to the diet in rats significantly suppressed the peroxidation level by 7-85% (plasma) and 24-72% (liver) as compared to beta-carotene (51-76%, 33-65%) over a period of 8 h. Fucoxanthin given topically or orally to mice once a day and UVB irradiation was applied for 14 days. Its topical application (1%) significantly suppressed mRNA expression of inflammation mediators. Oral application of fucoxanthin (10 mg/kg) significantly suppressed expression of COX-2, p75NTR, EP1 and MC1R. Also related : anti-inflammatory, anticancer, anti-obese, antidiabetic, antiangiogenic and antimalarial activities.

Andrographis Cold, Jaunice, Liver, respiratory Origin: Sabinsa (India) Source: Andrographis paniculata - leaves Bio-active ingredients: 10% Andrographolides (50% and 90% available) Dose : 250mg (of 10%) three times a day

Used in the treatment of the common cold - paracetamol like - to reduce its symptoms and duration. Inhibitor of Nitric oxyde production and synthesis of Endothelin (spot and pigmenting).

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28-29

Enzymatic activity of oral free SOD in the body is mainly to totally destroyed within few minutes, despite the source, and can so not promote the production of the SOD cascade by the cells. Usual studies based on free SOD cannot prove the efficacy, or at an incredibly high use (in-vivo). Thanks to its gliadin matrix, GliSODin® is orally effective unlike most existing SOD supplements. The gliadin is not an enteric protection – what should not be enough - but a real vehicle for the SOD, due to its specific bio-adhesive properties with the epithelial cells in the small intestine. The recommended daily dose is 2 x 100mg to 2 x 250mg a day. GliSODin® is supported by more than 20 – mostly published - studies : • Comparison of the GliSODin versus SOD alone, Protection of cellular DNA • Increase of the MED (resistance to UV induced erythema or redness), Skin’s protection against sun and improved tanning speed and quality • Regulation of inflammation and decreases oxidative stress • Significant decline in lactic acid levels • Reduction of fat synthesis and improvement of liver function • Preclinical on mice : Inhibition of endometriosis extension • Protective role on the statusof lipid peroxidation and antioxidant defense against azoxymethaneinduced experimental colon carcinogenesis Etc...

Garcitrin® & Citrin® Slows conversion of carbohydrates to fat Origin: Sabinsa (India) Source: Garcinia cambogia—fruits Bio-active ingredients: up to 60% Hydroxy citric acid Dose: 250mg of (-) HCA before meals

Citrin® and Garcitrin® are two grades extracted from the fruit Garcinia cambogia. Sabinsa has been the first to highlight the active (-HCA) and its use several years ago already. Citrin is a salt of the Hycroxycitric acid, named (-) HCA, and reduces the conversion of carbohydrates in stored fat and increasing the use of fat. Sabinsa has conducted numerous studies under the name Citrin ®, proving its effectiveness and safety. After initial use in the field of sports products (induced action on amino acids, detoxifying the liver, etc ...), its properties have led to slimming and weight management. Another reported activity of the Citrin is the prevention of urinary tract stones. A beverage grade is available : Citrin K BG (potassium salt), totally soluble. Sabinsa has highlighted another molecule from Garcinia cambogia: the Garcinol, showing at a first glance anti-oxidant, anti carcinogenic or antimicrobial properties. Sabinsa has shown that the use of combined HCA and garcinol improves further the efficiency of HCA, the garcinol acting as a vector for the HCA to target cells.

Amla Saberry™ Digestion, Blood sugar and Adaptogen Nutricosmetics : Melanogenesis inhibition, UV Protection , Hair care Origin: Sabinsa (India) Source: Emblica officinalis - fruits (Amla) Bio-active ingredients: 10% b-Glucogallin, 50% gallates (tanins) Dose : 90mg three times a day

Water extract of Emblica officinalis (Amla). The extract is widely used in digestive support and immune support applications (adaptogen). Blood sugar level. Liver support - b-Glucogallin has been studied by Sabinsa (metallo-proteinase inhibitor, studies : detoxification, e.g. heavy metals, pesticides). Amla helps in maintaining the anti-oxidant enzyme levels in the system. A beautiful fruit, well known for its rejuvenative properties . A light-colored powder prepared from the fruits of Emblica officinalis, commonly known as amla, and standardized to contain 10% ß-glucogallin. Benefits of this component can largely be attributed to it’s potent antioxidant capabilities which is substantiated by its oxygen radical absorbance capacity (ORAC) value ORAC hydro: 267 800 µmol TE/100g / Orac lipo: 400 µmol TE/100g HORAC: 34 500 µmol CAE/100g / NORAC: 90 00 µmol TE/100g SORAC: 10 200 µmol kunitsSODeq/100g / SOAC: 135 100 µmol VitE/100g

Forslean®

Pomegranate Punicic CLnA

Helps to increase lean body mass, while decreasing body fat Origin: Sabinsa (India) Source: Coleus forskholii - roots Bio-active ingredients: 10% forskholin (oral grade) to 98% forskholin (cosmetic grade) The daily dose is 2 x 250 mg/d orally. Topical use is from 0.1% to 0.5% of a topical formulation, such as an ointment, cream or lotion

Punicic acid to support healthy body weight Origin: Nektium (Spain) Source: Punica granatum – seeds Bio-active ingredients: Punicic acid ≥ 70 or 80% Dose : 2-3 x 150mg

Forslean is the patented extract from Coleus forskholii developed and proved by Sabinsa. Very well documented and subject of numerous studies showing the increase of the Lean Body Mass, and the use of fat by its action on the Adenylate Cyclase. It is used in nutrition, cosmetics or nutra cosmetics (toning of skin). It received first prize several times.

Punicic Acid (PA) is structurally related to Conjugated Linolenic Acid (CLnA). Conjugated fatty acids have attracted quite an interest because of their potential beneficial biological effects. Obese rats with hiperlipidemia experienced a significant decrease in hepatic tryacylglycerol deposits, as well as levels of monounsaturated fatty acids, when fed with diets rich in Punicic acids.

Pomegranate P40P Berberine Statin-like, Prostate, antimicrobial Origin: India Source: Berberis aristata Bio-active ingredients: Berberine HCl Dose : 50mg three times a day

Used and recognized for more than 2500 years in Chinese pharmacopoeia and Ayurvedic medicine, the berberine was traditionally recommended to reduce blood sugar levels and to lose weight. But berberine also has many other benefits, including acting on the cardiovascular system and intestinal flora. The therapeutic effects of statins and berberine on the hyperlipemia have been widely studied.

Shagandha® / Ashwagandha Adaptogen, Rejuvenative tonic, also Antispasmodic and Anti inflammatory, Psychological endurance (NSIR), Hair Origin: Sabinsa (India) Source : Withania somnifera roots Bio-active ingredients: Withanolides [>2.5% HPLC] Dose : 300 mg per day

Orange Hesperidin / Flavonoids Blood circulation, Antioxidant Origin: Nektium (Spain) Source: Citrus sinensis – fruit skin (from organic orange) Bio-active ingredients: 80% Hesperidin or 35% Flavonoids

The flavanone glycoside hesperidin is known for its use for the treatment of the fragility of the blood capillaries (varicose veins, hemorrhoids, poor circulation venous stasis, venous thrombosis) by preservation of the integrity of the blood vessels as well as the reinforcement of the network of capillaries, and increase the blood volume. It may also reduce inflammation It is a powerful antioxidant, which makes it very interesting to optimize the ocular health and to protect the cardiovascular system from the oxidative attacks. Processed from organic orange.

Pomegranate is lauded since old ancient times as having many health benefits. Pomegranate´s potential benefits span many areas such as cardiovascular disease, diabetes, and cancer to name a few. It is widely accepted nowadays that the beneficial health effects of fruits and vegetables in the prevention of disease are due to the bioactive compounds they contain. Pomegranates contain significant amounts of said substances, such as phenolic acids, flavonoids, and tannins assuring them considerable nutritional value. Nektium extracts from mashed fruits taking care to preserve the Punicalagin - as in the fruit - and not Ellagic in the P40P (one molecule of punicalagin is hydrolyzed to two ellagic acids). Additional studies show a stimulation of the growth of healthy bacteria in the gut, and a anti-inflammatory properties (action on COX-2 and lipoxygenase)

Licorice DGL Deglycyrrh. Digestion, Immunity Origin: Sabinsa (India) Source: Glycyrrhiza glabra Dose : 380 mg 2-3 times / day

Treatment of gastric and duodenal ulcers. It is made from licorice from which the glycyrrhizin has been removed. Immune support and management of gastrointestinal problems.

Lycopene Prostate, cardiovascular, antioxidant, sun protection Origin: India Source: Tomato, Lycopersicon esculentum - Fruit Bio-active ingredients: Lycopene 10% by UV Dose : 250mg twice a day in capsule form

Lycopene, a Carotenoid , is transported by the lipids of the blood and accumulates in certain organs, in particular in the liver and the prostate. Epidemiological studies have shown that lycopenes are powerful antioxidants that can provide protection against oxidative damage and limit the development and progression of many tumors (included prostate, lung, colon, beast). Insoluble in water, liposoluble. Prostate: Studies showed that supplementation of 15mg of lycopene twice a day reduced the proliferation of the cancerous tumor. Lycopene would reduce the symptoms that are associated with prostate cancer, such as pain and urination disorders. Cardiovascular: Like other antioxidants, it prevents LDL (bad cholesterol) from oxidizing. Oxidized LDL is known to promote atherosclerosis or clogging of the arteries and may lead to myocardial infarction and stroke. Sun: Lycopene, in combination with other carotenoids, reduces the harmful effects of ultraviolet radiation on the skin. It is known that excessive exposure to sunlight can damage skin cells and even lead to skin cancer.

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NUTRITION

Ashwagandha, Withania somnifera, is one of the best-known Indian botanicals that has been used in Ayurvedic preparations since the Vedic era, dating back 3000-4000 years. In Ayurveda, ashwagandha is classified as a rasayana, meaning “rejuvenative tonic”. Also referred to by the common name of Indian ginseng (but nothing to see with). Ashwagandha have multiple therapeutic properties: tonic action, reduce stress and relieve anxiety, neurons protection, increase GABA A receptors activity, delay the process of cellular aging, reduce blood sugar levels, improve libido and decrease the activity of acetylcholinesterase and slow down development of tumor cells. Strengthen the cerebral cortex, the adrenals, the hypothalamus, the heart and the liver. With Shagandha™, Sabinsa assure the authenticity of its ashwagandha extract by complying with the US Pharmacopoeia-National Formulary monograph (USP-NF). The company has selected Alkemist Labs, botanical specialist, to test the ingredient and confirm its compliance with the USP monograph.

Cardiovascular health, Antioxidant, Weight, Inflammation Origin: Nektium (Spain) Source: Punica granatum – mashed fruits Bio-active ingredients: Total punicosides ≥ 40%, Punicalagins A+B ≥ 30%, Total polyphenols ≥ 50% Dose : 2 x 100mg to 300mg (based on the spectrum of the fruits). Study published

Promond®

Gymnema GS4+®

100% natural and vegan protein obtained from almonds Origin: Sabinsa (India) Source: Prunus amygdalus Bio-active ingredients: 50% Proteins

Weight Management, Blood glucose Origin: Sabinsa (India) Source: Gymnema sylvestre leaves Bio-active ingredients: Gymnemic acids 25% or 75% Dose : 75mg (based on 75%) twice a day

Promond almond protein is a water-soluble 50% protein sourced from Indian variety of almonds (water extract). It has a complete amino acid profile. It is having a complete array of amino acids, including high levels of branched-chain amino acids (BCAAs). Although its amino acid profile is similar to whey and pea protein, is particularly high in glutamate, arginine, aspartate, and phenylalanine. Those concerned about almonds grown in California because of heavy water use in drought conditions won’t have to worry, Promond is made from almonds sourced in northern India.

Bacopin®

Attention, stress, memory, circulation Origin: India Source: Bacopa monniera Bio-active ingredients: 20% and 50% total Bacosides (as A & B), ethanolic extract Dose : 3 x 50 mg (children) to 3 x 100 mg (adult)

• Bacosides can revitalize the intellectual functions in children and calm down the stressed mind, allowing for better brain function • an be effective in elevating brain function by increasing concentration and more finely honing mental focus • Anti-Convulsant and anti-cataleptic actions. Anxiety Neurosis Ayurvedic uses : Memory Enhancing, Anti-inflammatory, Analgesic, Antipyretic, Sedative, Anti-ulcerogenic, Anti-epileptic.

Centellin®

NUTRITION

Respiratory, Stress, Blood circulation Origin: Sabinsa (India) Source: Centella asiatica Bio-active ingredients: Terpens and asiaticosides

Traditionally use (also known as Gotu kola). Recent studies have validated its uses, especially as a psychotropic n the treatment of various mental disorders, such mental fatigue, epilepsy and hysteria. Centella asiatica could significantly improve the IQ of children and reduce the level of anxiety in adults. Venotonic activity linked to triterpens.

FruitOx® In-vivo cellular antioxidant, ORAC > 1 000 000 µmol TE / 100g Water soluble, no carrier, production on request Origin: Nektium (Spain) Source and Bio-active ingredients: polyphenols and other actives compounds from plum, blueberry, apple, pomegranate, grape Leaves Dose from 3 x 100mg to 3 x 900mg (full spectrum for total lack of antioxidant) FruitOx® is a formulation based on polyphenols from different fruits and plants, developed by Nektium with the idea of a soluble product, fruits based, antioxidant, and offering a wide spectrum of anti-radical properties (hence the selection of only certain specific polyphenols from each source) and stimulation of cellular defenses. FruitOx® has been controlled at its ORAC value of more than 1 000 000 (Trolox equivalent / 100g). A clinical trial with FruitOx® significantly improved blood lipid profiles along with significantly decreased oxidative stress markers in 22 obese volunteers compared with those taking placebo. FruitOx® positively promotes the antioxidant genes (key genes expression) while the genes causing oxidative stress were suppressed. Then, it has been the subject of several studies and research such as the upregulation of the expression of opioid receptors in brain cells (OPRD1). Efficiency of pharmacologically-active antioxidant phytomedicine Radical Fruits in treatment hypercholesteremia at men.

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Gymnema sylvestre is an herb native to the tropical forests of southern and central India. Chewing the leaves suppresses the sensation of sweet. It may suppress levels of leptin, insulin, dyslipidemia, apolipoproteins, lipids, visceral fat pad weights, and oxidative stress in obese rats fed with HFD. This effect is attributed to the presence of the eponymously named gymnemic acids. G. sylvestre has been used as a natural treatment for diabetes for nearly two millennia. Gymnemic acids have antidiabetic, antisweetener and anti-inflammatory activities. The antidiabetic array of molecules has been identified as a group of closely related gymnemic acids after it was successfully isolated and purified from the leaves of Gymnema sylvestre. Later, the phytoconstituents of Gymnema sylvestre were isolated, and their chemistry and structures were studied and elucidated. Increase production of Insulin in pancreas.

ActiCissus® Anti-osteoporotic, bones repair, Weight, Tonic Origin: Sabinsa (India) Source: Cissus quadrangularis - aerial part Bio-active ingredients: 10% Ketosterones, 1% Natural Calcium Dose : 200 to 500mg per day

Also named “devil’s bones” because it “joints” bones much quicker after fracture, Cissus q. is used in Ayurveda medicine as a general tonic, analgesic and to heal bone fractures. • Anabolic and/or androgenic properties (glucocorticoid receptors) • Weight loss (in combination with Irvingia g.) • Analgesic : effective Analgesic vs aspirin; Cortisol reducer (Cortisol Antagonist) • Anti-osteoporotic : femur ossification, mineralization, osteoblastic activity • Anti-ulcerogenic : comparison with Ranitidine, decrease gastric secretions • Anti-oxidant : comparison to BHT, inhibition of DPPH, in-vivo trials • Anti-microbial : trials versus tretracyclin

Shatavari Women health, Adaptogen Origin: Sabinsa (India) Source: Asparagus racemosus Bio-active ingredients: 20% Saponins Dose : 200mg - 300mg, twice a day

Shatavari, or Asparagus racemosus, has long been used in Ayurveda, the traditional medicine of India, as a tonic regenerator of the female reproductive system. It has several effects: • Attenuates premenstrual syndrome, allows to support difficult periods. • By increasing fertility, shatavari is useful for a woman wishing to conceive, as it acts as a uterine tonic and helps prevent miscarriages. • Aphrodisiac effect, it stimulates the libido. • When used during menopause, it soothes hot flashes (cooling effect). • Promotes lactation in women who have just given birth through its galactagogue effect (by increasing the amount of prolactin).

Cococin®

The ultimate nutrient Healthy cell growth, Blood circulation, Nutrient Origin: Sabinsa (India) Source: Cocos nucifera

Freeze dried coconut water solids (obtained from tender green coconuts), Cococin® is a natural reservoir of isotonic nutrients to promote tissue growth in the developing endosperm.

Capsallyl® / Capsaicin

SELENIUM SELECT®

Bioavailable and safe form of Selenium

Support healthy muscle mass Origin: Sabinsa (India) Source: Capsicum annuum, Brassica Bio-active ingredients: Capsaicinoids (Allylisothiocyanate) Dose : 250mg twice a day in capsule form

Capsaicinoids from Capsicum annum (with 1% Allylisothiocyanate in AITC), for weight management (actives are both pungent ingredients, well known to induce thermogenesis and decrease adipose tissue weight and TG) and to treat aches & pains in muscles & joints. Capsallyl is supplied in Beadlets, enteric coated. Capsaicin beadlets 2% enteric coated, to 90% total capsaicinoids HPLC

L-Selenomethionine, Immunity, Cognitive decline, Hair, Antioxidant, Origin: Sabinsa (India) Source: Synthesis Bio-active ingredients: 5000ppm to 39% of elemental Se Dose : 50 - 200 mcg a day of elemental selenium

Selenium as LSM is completely bio-available, created by Sabinsa: the Selenium in the protein structure protects DNA from oxidation more efficiently than the original sulfur in methionine. The carbon-selenium bond is more easily broken during photochemical reactions than the carbon-sulfur bond. Complexed with the amino acid methionine Preferred form of selenium due to organic nature More and readily absorbed than inorganic forms Sodium content less than 0.1%. Selenium is a vital trace element nutrient with multiple roles in the growth and functioning of living cells in higher animals and humans. This element is unevenly distributed in the earth’s crust. Almost all of the selenium in animal tissue is found in the proteins. Some of these proteins contain stoichiometric quantities of selenium and are known as selenoproteins. Other proteins contain variable amounts of selenium (which substitutes sulfur randomly in the original protein) and are known as selenium-binding proteins.

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a-Lipoic acid Co-factor for number of key enzymes, Antioxidant Origin: Sabinsa (India) Source: Synthesis (pure) Dose : 25-50 mg Three times / day

Alpha-Lipoic acid plays as a co-factor for a number of key enzymes that help in the conversion of glucose, fatty acids and other energy sources into ATP. It helps to regulate blood sugar and prevent diabetic retinopathy & cardiopathy. Alpha-Lipoic acid is an antioxidant that prevents free-radical damage, and is thus important in the life processes of all living organisms. R-a-Lipoic acid is the rancemic form in which alpha lipoic acid is produced naturally in the body, and the anti-inflammatory activity of the R form could be up to 10 times higher than that other alpha lipoic acid.

Other Organic elements

LeanGard

Chromium, Vanadium, Zinc monomethionate, Magnesium, Cooper, Molybdenum

Healthy body composition, Weight management Origin: Sabinsa (India) Composition: Forslean, GarCitrin and BioPerine Bio-active ingredients: Forskolin, hydroxycitric acid, garcinol, piperine Dose: 500 mg per dose twice a day LeanGard is a proprietary blend of natural extract :Forslean build lean body

mass maintain healthy body composition • Garcitrin supports satiety, weight management and lean body mass • BioPerine is a natural bioavailability enhancer

Chromium Polynicotinate Origin: Sabinsa Assay : Hydrated Cr(111) Dinicotinic acid complex a minimum of 14% chromium organically bound to a mimimum of 77% nicotinic acid on anhydrous basis

Chromium is an essential trace mineral nutrient and plays a critical role in maintaining normal health and well-being (optimal functioning of insulin, maintaining serum cholesterol levels, management of obesity). Chromium supplementation in this bioavailable organic form will also help to offset stress associated immunosuppression.

Bis-glycinato oxo vanadium Origin: Sabinsa Assay : minimum of 20% elemental Vanadium

Our products, articles and main studies on becarre-natural.com

Zinc, Potassium, Cooper, Molybdenum, Magnesium Origin: Sabinsa

Various forms are processed by Sabinsa : Copper lysinate, Molybdenum Methionate, Zinc Picolinate, … besides minerals combined with plant extracts (for instance Citrin K, Citrin Mg, Citrin Ca, Citrin CaK, Cissus, etc…).

KEY PRODUCTS Anti-inflammatory, Bones, Joints, Arthritis

Weight control, Satiety

Digestion, Apetite, Transit, Colon

Memory, Attention, Anxiety, Stress

Wellness, Family health, Adaptogen

Cardiovascular health, Blood circulation

Immune System, Cold, Flue, Antibacterial

Skin, Hair, Nails

Sun protection, Tanning

Blood sugar, Cholesterol

Antioxidant, ADN

Sport, Performance, Tonic, Energy

Water soluble, Instant

Minerals, Synthetic, Intermediates

NUTRITION

A new form of organically bound vanadium, Bis-glycinato-oxovanadium (BGOV) is a vanadium complex with the amino acid glycine, containing 23% elemental vanadium on anhydrous basis. Vanadium belongs to a group of biologically important metals like chromium, iron, cobalt, copper and zinc, which are termed as «transition» elements, which have a natural propensity to form complexes with organic compounds called «coordination compounds».

Latin Names Zynamite®

Main studies published in 2020 Plant

Name

NUTRITION

Adhatoda vasica Adhatoda Aesculus hippocastanum Venocin Ajuga turskestanica Ajuga Allium sativum Selenium Alpinia galanga enXtra Ananas comosus Bromelain Andrographis paniculata Andrographis Anethum sowa Dill Apium graveolens Celery Asparagus racemosus Shatavari Bacillus coagulans LactoSpore Bacopa monniera Bacopin Berberis aristata Berberine Beta vulgaris Beet Root, Sabeet, RedNite Boswellia serrata Boswellia Brassica juncea Mustard Camellia sinensis Green Tea Capsicum annuum Paprika Carica papaya Papain Cinnamomum cassia Cinnamon Cissus quadrangularis ActiCissus Citrus limon Lemon fiber Citrus sinensis Orange Clupea harengus harengus Sementis Coleus forskohlii Forslean Commiphora mukul Gugulipid Crocus sativus Satiereal Cucurbita pepo Pumpkin Curcuma longa C3 Complex Curcuma longa Starmeric Curcumis melo, Wheat gliadin GliSODin Eclipta alba Eclipta Emblica officinalis Amla Enzymes Lactase Enzymes Digezyme Foeniculum vulgare Fennel LeanGard

Bioactives

Vasicine,Alkaloids Terpens, Escin Ecdysteroids,Turkesterone Selenium Polyphenols,Tannins,Glycosides Bromelain Andrographolides Fatty Acids Butylphthalide, Fatty Acids Saponins Prebiotic spores Bacosides Berberine HCl, Sulfate Nitrates, Betalain AKBBA, Boswellic Acids, Polysaccharides Allylisothiocyanate Catechins,Epigallocatechin,Polyphenols Capsaicin, Chilitenoids Papain Polyphenols,Proanthocyanidins Ketosterones,Minerals Dietary Fiber Hesperidin, Flavone, Bioflavonoids

Forskolin Guggulsterones Safranal,Picrocrocine,Crocine Fatty Acids Curcuminoids, Tetrahydrocurcuminoids Resistant Starch Superoxydismutase,Enzymes Wedelolactone beta-Glucogallin,Tannins,Fibers Lactase Lactase, Lipase, Protease, Cellulase, Amylase Fatty acids Forskolin,Hydroxycitric Acid,Garcinol,Piperine Glucosamine,Boswellic ArthriBlend Acids,Curcuminoids,Piperine Plants Formulation Hot Sip Glycyrrhizinic Acid, Piperine, Alkaloids ZeaLutein Lutein, Zeaxanthin, Piperine, Triphala Tannins,Polyphenols Sage Ursolic Acids / Oleanolate Glycyrrhiza glabra Licorice Glycyrrhizinic Acid, Fibers, Glabridin Griffonia simplicifolia Griffonia 5-HTP (Hydroxytryptophan) Gymnema sylvestre Gymnema Gymnemic Acids Hordeum vulgare Barley beta-Glucans Inula racemosa Inula Lactones Kaempferia galanga Galanga Cinnamate Laminaria japonica Fucoidan Polysaccharides,Polyphenols,Fucoidan Lycopersicon esculentum Lycopene Lycopen Magnolia officinalis Magnolia Magnolol,Honokiol Mangifera indica Zynamite Mangiferin Melia azadirachta Neem Oil,Limonoid, Bitter principles Mineral pitch Shilajit Fulvic Acid Momordica charantia Momordica Bitter Principles,Charantin Momordica cochinchinensis Lycopene Lycopen,beta-Caroten Moringa oleifera Moringa Saponins,Rutine, Proteins,Magnesium Mucuna pruriens Mucuna L-Dopa Nigella sativa Nigellin Thymoquinone,Fatty Acids Ursolic, Eugenol,Caryophyllene,Ursolic, Ocimum sanctum Tulsi Tannins,Polyphenols Olea europaea Olive Oleuropein Phaseolus vulgaris Fabenol Enzymes,Inhibitor Phyllanthus amarus Phyllanthus Bitter Principles,Phyllanthin,Tannins Piper nigrum BioPerine Piperine Polygonum cuspidatum Resveratrol Resveratrol,Stilbens Prunus africanus Pygeum Sterols Pterocarpus marsupium Silbinol Stilbens,Pterocarposide Pterocarpus marsupium Pterosol Tannins,Glycosides Punicic acid CLnA, Punicosides, Punicalagins, Punica granatum Pomegranate Polyphenols, Ellagic Rhodiola rosea Rhodiolife Rosavins,Salidroside Carnosic Acid,Ursolic Acid,Rosmarinic Acid, Rosmarinus officinalis Rosemary Terpens Saccharum officinarum Policosanol Policosanol Salacia reticulata Salaretin Mangiferin,Terpens,Saponins Salvia officinalis Sage Ursolic Acid,Oleanolate Sambucus nigra Elderberry Anthocyanins,Polyphenols Schisandra Chinensis Schisandra Schizandrol,Schizandrin Serenoa repens Saw Palmetto Fatty Acids Alpha Lipoic Alpha Lipoc Acid Diindolylmethane (DIM) Diindolylmethane (DIM) Glucosamine Glucosamine Synthesis I3C Indole - 3 - Carbinol (I3C) Resveratrol Resveratrol,Stilbens Tagetes erecta Lutein Lutein Terminalia arjuna Arjunolic Arjunolic Acid, Saponins Terminalia belerica Terminalia Tannins Terminalia chebula Terminalia Tannins Tinospora cordifolia Tinofolin Bitter Principles Tribulus terrestris Tribulus Saponins,Protodioscin,Steroids Fibers, Galactomannans,Fenumannans, Trigonella foenum graecum Fenugreek Saponins, Steroids Undaria pinnatifida Fucoxanthin Fucoxanthin Vitex negungo Vitex Agnuside Withania somnifera Ashwagandha Withanolides,Alkaloids,Withaferin Zingiber officinale Ginger Gingerols Seleno-methionine, Zinc monomethionine, Methyl Selenocysteine Synthetic or enriched plants Minerals Chromium Picolinate, L-Seleno-Methionine, Methyl Selenocystein, Molybden Methionate, Gamma-Glutamyl-L-Selenomethionine, N-Acetyl L-Cyst., etc… "

18 - BECARRE Natural - October 2020

Zynamite® clinical trial demonstrates sustained effect on brain functions from a single dose. One dose of Zynamite® is enough to significantly improve performance in a range of cognitive tasks including enhanced attention and memory, reports new clinical data. à Double-blind, placebo-controlled cross-over design with 70 healthy women and men aged 18 to 45 à Each received a single dose of 300mg Zynamite® and a matched placebo (on separate occasions) à A battery of cognitive tests were performed pre-dose, at 30 minutes, 3 hours, and 5 hours post dose à Significant improvement performance accuracy of Computerised Mental Performance Assessment System (COMPASS) assessment battery, including enhanced performance in the Attention and Memory assessments. à Effects were sustained throughout the study – from 30 minutes after dose to 5 hours post dose

Nootropic effect of Zynamite® in publication The latest published article “Central Nervous System activities of extract Mangifera indica L. outlines the nootropic effect of Zynamite® based on the studies that Nektium has performed. à Mangiferin was found for the first time to be a catecho-O-methyltransferase inhibitor à Mangiferin and Mangifera indica extract (MLE Zynamite) for the first time have been shown to increase long-term potentiation in vitro in a hippocampus slice model à Mangiferin and MLE have shown to cause a similar pattern of change in EEG in vivo à In pilot translational RTC clinical trials MLE: • Was shown to change the pattern of brain electrical activity significantly during cognitive challenges • improved reaction time compared to placebo • improved score of fatigue on the Profile of Mood States questionnaire • was well tolerated, no change compared to placebo in blood pressure, heart rate and heart rate variability

Zynamite® in Combination with Quercetin Attenuates Muscle Damage and Pain and Accelerates Recovery after Strenuous Damaging Exercise

à In a double-blind cross-over clinical study with a single 420 mg dose of synergistic combination of Zynamite® + Quercetin administered one hour before exercise, the peak power was improved by 3.8% versus placebo. à In a separate study, a single 420 mg dose of Zynamite® + Quercetin attenuated the soreness elicited by competitive exercise and accelerated the recovery of muscle performance.

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Boosting mental and physical energy with Zynamite® Zynamite® is the multi-award winning patented botanical ex trac t, clinically documented to provide significant sustained boosts to mental and physical per formance.

Benefits

Cognitive health (clinical studies) + Rapid onset of mental activation + Faster reaction time + Increase performance + Focus and concentration

Sport Performance (clinical studies) + Sustained energy + Increase power + Doping free

Safety & Information

+ Own safety data : AMES, 14-day and 90-day oral toxicity, NOAEL... and new soon coming. + GMO, BSE/TSE free, Doping free, DNA barcode, Vegan, Kosher, Halal, traditionally used in food, registered in different countries in Europe, Australia, New Zealand, USA (self GRAS i.p.)

ynamite® is the healthy way to replace caffeine with a botanical ingredient without the side-effects of caffeine and actually works better than caffeine, while retaining the experiential effect. Zynamite® is the Nektium’s patentpending proprietary Mangifera indica extract, getting the Nutraingredient award in 2018, 2019, and ESSNA award Best free-from product’ and ‘Runner-up Game changer’

Zynamite® gives sustained mental and physical energy and focus without any negative side effects. What do tired people need ?

Stressed or fatigued people lack enthusiasm and mostly feel exhausted and desperate. What they desire is a sustained boost of energy that improves physical performance, concentration, attention, alertness and mood.

The problem with caffeine

Because of the mental energy and focus provided by caffeine, billions of cups of coffee are consumed daily to assist people to cope. However, caffeine is addictive and most

Product Features Botanical origin Mangifera indica mango leaves Bioactives ≥ 60% mangiferin Cultivated or wild Cultivated Cropping pattern Conventional Appearance Fine powder Pack size 25kg

Taste Mild taste Recommended serving 100-200mg once to twice a day Quality GMO free, BSE/TSE free, DNA-barcode, Doping free Product certification Kosher, Halal

2019 EUROPEAN SPORTS NUTRITION AND HEALTH INGREDIENTS NEW PRODUCT INNOVATION AWARD

people consume too much of it and suffer from its negative side effects and withdrawal symptoms.

Caffeine in Sports Products Many sports products contain caffeine to increase alertness and boost physical energy but these products inevitably lead people to experience jitters, anxiety, irregular pulse, insomnia and nausea - all physical and mental issues for concern, especially for sports performers. Zynamite® is the ideal alternative.

What is Zynamite®? Zynamite® is Nektium’s patent-pending proprietary Mangifera indica extract standardized to ≥ 60% mangiferin. Mangifera indica, the common mango tree, is cultivated extensively in the tropical and subtropical regions of the world. Infusions and decoctions of mango leaves are used extensively in traditional health systems and have been known for centuries for their health benefits.

How does Zynamite® work? Zynamite® activates brain waves and increases Long Term Potentiation in a remarkably similar pattern to caffeine. While caffeine antagonizes adenosine receptors, Zynamite® acts non-selectively to stimulate brain activity. The key active, mangiferin acts non-selectively to modulate multiple physiological and CNS targets.

Bio-actives of Zynamite® The active principle of Zynamite® is mangiferin, a natural flavone polyphenol. Ultra-highperformance liquid chromatography (UHPLC) with photodiode array detection (PDA) is used to provide high-resolution separation, identification and quantification.

Mental Activity Zynamite® has remarkably similar mental

activation activity to caffeine in studies of brain electrical activity in vivo. The pattern of electrical activation for both Zynamite® and caffeine are remarkably similar in the brain frontal cortex and in the hippocampus.

(Preclinical study, Collaboration with Professor Wilfried Dimpfel, Justus- Liebig -University, Giessen, Germany)

Mental Energy The effect of a single dose of Zynamite® as caffeine-replacement was tested in 32 participants in a double-blind, randomized, placebo-controlled, 2-armed study with parallel design. Calculation performance Significantly improved cognitive performance. Reaction time Significantly faster reaction compared to placebo. (two clinical studies, Collaboration with Professor Wilfried Dimpfel, Justus- Liebig -University, Giessen, Germany)

Sport Performance The effect of 48 hour intake of Zynamite® on performance was tested in two sports formulations in a doubleblind, placebo controlled crossover cycling trial in 30 young physically active athletes2. Mean peak power output reached in each sprint was significantly higher for both Zynamite® formulations compared to placebo. Peak power output developed in the 15-s sprint performed immediately after ischaemia was significantly higher for Zynamite®. Brain oxygenation measured with spatially resolved nearinfrared spectroscopy in the female group of thirteen women was significantly higher for both Zynamite® formulations.

Sourcing Zynamite® is rooted in a history of traditional use. Nektium spent years understanding how different traditional cultures have used a variety of herbs to boost concentration and mental focus– from the Amazon to India, China, and Africa. Their search led them to develop the specific profile of extract that now make up the patented blend in Zynamite®. Zynamite® is sourced by selectively pruning cultivated mango trees in the tropics meaning it is not only a natural alternative to caffeine, but as a by-product of mango production is also an ecologically sustainable one.

FOCUS A rich source of all-natural, vegan, and versatile protein à Free-flowing à Water Extract, water Soluble à Good taste

Promond®

romond™ is a 100% natural and vegan protein obtained from almonds and standardized to contain not less than 50% protein. This off-white to cream, free-flowing powder has good taste compared to other protein powders. It is having a complete array of amino acids, including high levels of branched-chain amino acids (BCAAs). Although its amino acid profile is similar to whey and pea protein, is particularly high in glutamate, arginine, aspartate, and phenylalanine. Almonds (Prunus amygdalus) are incredibly popular nuts worldwide, which belong to species related to cherries and plums, and are native to Middle East and South Asia. These highly nutritious and extremely healthy nuts are a rich source of proteins, dietary fiber, and other micronutrients.

Gugulipid™

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Commiphora mukul to regulate cholesterolemia and not only. The proven extract.

ugulipid® is the extract from Commiphora mukul developed ad launched by Sabinsa even before 1997. It’s standardized in Guggulsterones E & Z at 2.5% or 7.5%. Clinical studies have shown improvement in the lipid profile in the blood, lipoprotein levels and markers of inflammation. Preclinical studies have shown that all fractions of C. mukul lower serum cholesterol, but the steroid fraction (guggulsteronnes-E and guggulsteronnes-Z) is more effective. In addition, the extract is effective in reducing serum and liver lipid levels. These results are comparable to lipidlowering agents (clofibrates) at the same dose. Several studies by Sabinsa, Gugulipid® vs placebo in hyperlipidemic patients have shown the following results: à Cholesterol, TG, VLDL: significant decrease à HDL: significant increase à Increase in α-lipoprotein à Decrease in β-lipoprotein à Decrease in hs-CRP protein (inflammation) and MDA (oxidative stress) Sabinsa also highlighted the mechanism of action of guggulsteronnes to improve the elimination of 20 - BECARRE Natural - October 2020

cholesterol and the inhibition of its biosynthesis: à Increased excretion of cholesterol and bile acids à Increased level of catecholamine à Inhibition of HMG CoA reductase enzymes Other studies have also shown that guggul extract can stimulate thyroid function, which can also act on the regulation of cholesterol levels: à Increased peroxidase and thyroid proteolytic activity (after 6 days) à Increase in iodine concentration after 10 days In Ayurverdic medicine guggul means: “that which protects from the disease”. In Sanskrit, guggulu means: “that which protects from disease”, in ayruvedic medicine it is said that guggul releases the “subtle channels” which awaken consciousness and refine perceptions.

Guggulsterones The active ingredients responsible for the maintenance of healthy cholesterol levels are the guggulsterones, specifically guggulsterone-E and guggulsterone-Z. The study of the pharmacology of oleogum resin dates back to the 1960’s in India, where the gum was evaluated for its potential in the treatment of elevated blood cholesterol. Guggulsterones are postulated to enhance the elimination of cholesterol and to inhibit its biosynthesis. Several mechanisms of action have been proposed which directly or indirectly affect cholesterol metabolism.

a.a. Glu His Ile Leu Lys Met

/100 26,3 4,0 3,5 6,3 2,5 0,2

a.a. Phe Thr Trp Val Ala Arg

/100 4,5 1,9 0,4 4,1 4,2 10,6

a.a. Asp Cys Gly Pro Ser Tyr

/100 12,0 0,2 8,5 5,1 3,1 2,6

In recent years, extensive research on almonds has proved that this versatile nutrient food exerts an array of health benefits, including healthy heart support [maintaining healthy cholesterol levels (Wien et al., 2010; Berryman et al., 2015), it may reduce risk of coronary artery diseases (Jenkins, 2002 & 2006; Hu et al., 1998) and oxidative stress (Li et al., 2007), healthy blood sugar levels (Jenkins et al., 2006) and healthy weight management, and related complications (Wein et al., 2003; Berryman et al., 2015; Abazarfard et al., 2014).

The cardiovascular response for cholesterol regulation, reduction of stored fat, and inflammation. Recommended dose: 12.5 to 25 mg guggulsterones 3 times / day Grades at 2.5% or 7.5% guggulsterones by HPLC

Gugulipid Clinical Studies

Various studies are available. Gugulipid® is supported by Phase I, Phase II and Phase III studies organized by the CDRI, India. Also a double-blind, cross-over study compared Gugulipid® to the lipid lowering drug Clofibrate. In US : Randomized, Double-Blind, Placebo-Controlled Trial Evaluating the Safety and Efficacy of Gugulipid® in Americans with Hypercholesterolemia - University of Pennsylvania, and Serum lipid response during 12 weeks of Gugulipid® therapy in subjects with mild-to-moderate hyperlipidemia.

Tinofolin® Tinofolin® is the extract by Sabinsa of Tinospora cordifolia, one of the extremely valued medicinal plants of Ayurveda, commonly called as “Guduchi”. Guduchi means one which protects the entire body and is attributed to its ability to impart youthfulness, vitality, and longevity.

n Ayurveda, Tinospora cordifolia is believed to have rejuvenating, tonic, anti-aging, longevity and aphrodisiac properties. Traditionally well known for its anti-stress activity. The plant has been validated for its anti-inflammatory, antidiabetic, anti-arthritic, anti-stress, anti-allergic,

FOCUS Capture memories forever

anti-malarial, immunomodulatory and hepatoprotective activities.

Enhances verbal learning and memory and logical memory (of immediate and short term type) compared to placebo in healthy volunteers. Tinofolin® was evaluated for its ability to improve visual memory, logical memory, verbal memory, attention span and concentration in a placebocontrolled double-blind study involving 30 human volunteers of age 18-30 years for 21 days. The supplementation (500 mg/day) significantly improved verbal learning (placebo: -1.2±1.9 & Tinofolin®: 6.9±2.5; p < 0.05) and memory and logical memory (placebo: 5.1±6.1 & Tinofolin®: 26.6±6.7; p<0.05) compared to control. There were no significant untoward effects reported during the trial. Hence, Tinofolin® at 500 mg per day enhances the verbal learning and memory and logical memory (of immediate and short term type) compared to placebo in healthy volunteers.

In another double-blind placebo-controlled study in 30 healthy human volunteers for 21 days, 500 mg of Tinofolin® showed no change in the hematological and biochemical parameters. Hence, Tinofolin® can be considered as safe for human consumption. The mechanism of action is not yet clear and Sabinsa plans to conduct further studies to better understand them. Here are the conclusions of their pre-study: Tinospora cordifolia, used for its immunomodulatory properties, could have improved cognition by increasing IL-1 synthesis. The second mechanism may be due to an increased synthesis of acetylcholine (an important neurotransmitter in memory learning) by supplementation with choline, which is an important active constituent of Tinospora cordifolia. Efficacy of Tinospora cordifolia on Learning and Memory in Healthy Volunteers: A Double-Blind, Randomized, Placebo Controlled Study

BECARRE Natural - October 2020 - 21

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Satiety and Good Mood

atiereal® is a patented clinically proven satiety ingredient derived from saffron stigmas. The satiated feeling induced by Satiereal® encourages weight loss while eliminating frustration. This product has a unique mechanism that not only creates satiety but helps avoid snacking and compulsive eating behaviors, which in turn leads to reduction in weight and inches .

management in 60 women at the dose of 176,5mg/day; it shows that Satiereal® increases satiety, decreases hunger, reduces sugar cravings (-78%), and reduces compulsive snacking between meals. Women taking Satiereal report decreased hunger. 80.7% of women taking Satiereal® lost weight in two months. Notably, Satiereal® group lost preferentially fat mass, instead of lean mass.

What is unique about Satiereal® saffron extract is that it targets emotional behaviors : à It decreases your desire to snack à It decreases your desire for sugary foods à It increases your sense of satiety or fullness (resulting in better portion control) à It provides a good mood à It can be used in any form or food

Dosage : 2 x 88.25mg a day Water soluble, homogeneous without change after heat treatment (110°C, 20mns for instance) for beverages,…

Satiereal® is derived from the stigma of saffron crocus, Crocus sativus L, which is cultivated throughout the Mediterranean region. Historically, saffron was prized as a culinary spice, colorant and dye, and as a medicinal herb to ease digestion or to treat depression). These medicinal uses are usually curtailed by saffron’s high cost. However, Satiereal® is made from a special crocus cultivation rich in active saffron constituents, so it is cost effective as a weight-loss formula.

Trademark awareness : SATIEREAL® - on internet, plenty of testimonials, YouTube videos… consumer will search before purchasing and will rely on and look for the brand.

Not a common Saffron ! Satiereal®, a specific Saffron extract, works as a hunger suppressant and seems curb appetites by boosting serotonin levels. The satiated feeling induced by Satiereal® encourages weight loss while eliminating frustration. Capsules and Tablets with Satiereal® Instant stick for instance coffee or coated tea, with 9.81% of Satiereal® AWS per stick of 1.8g (formulation checked by Inoreal). Meal replacement : problems with meal substitute is the snacking in between ! Satiereal® provides a long feeling of satiety... better mood so feel better • Replacing 1 meal/day « weight management after weight loss » • Replacing 2 meals/days « weight loss contribution »

FOCUS HINTS

Specific Saffron Extract Satiereal® is a specific combination of Crocin (carotenoid dye, crocin (golden yellow-orange), Picrocrocin (flavor, bitter glucoside, a truncated version of the carotenoid zeaxanthin) and Safranal (aroma, less bitter), definitely more than a Saffron extract of Safranal. Inoreal noticed that the efficacy of the active principle needs them all together in a specific ratio : generic Saffron extract shows at same Safranal content shows a Inhibition of up to 10 times less.

Clinical Trials Clinical vs placebo 16 women, checked efficacy and tolerability of Satiereal® (2 x 88.25mg). 100% of supplemented women express the decrease of their food intake associated to a satiety feeling, to a decrease of the hunger feeling in the beginning of meals and a decrease of the meals duration. This is still persistent until dinner when Placebo shows an increase compulsive and reactive desire to eat Decrease of body weight and fat mass for the supplemented subjects versus the placebo group. Decrease of the desire and pleasure associated to the food intake mass for the supplemented subjects versus the placebo group. Double Blind vs placebo (Publ. Nutrition Research) Randomized double-blind versus placebo showed the efficacy of Satiereal® on weight 22 - BECARRE Natural - October 2020

Serotonin Reuptake Inhibitor Stress, anxiety, depression… influence food behavior, generally leading to compulsive overeating in reaction to stress. This phenomenon induces weight gain, subject feel guilty, trying to stop, thus generating stress for those who need consolation in carbohydrates intake !

Cereals Bars, biscuits:1,18% of Satiereal® / bar of 14.9 g

This is mostly due to low Serotonin levels. Satiereal® has been demonstrated to keep the neurotransmitter serotonin signal active, in other words serotonin re-uptake inhibition in neurons (mechanism of many antidepressants). It is probable that this serotonin re-uptake inhibition accounts for saffron’s use in treating depression and “melancholia” in the medicinal traditions. Saffron extract has been shown to be as effective as the tricyclic antidepressant drug imipramine and the SSRI fluoxetine (Prozac) in managing depression — with no side effects. Pharmaceutical SSRIs have varying effects on weight, but Satiereal helps keep excess body weight from accumulating. This is one advantage of Satiereal® over other dietary satiety ingredients: it needs only be used in small quantities, it is potent, it acts directly on neurotransmitters to relieve the cause of nibbling and hyperphagia, instead of just delaying it.

Candies, Chewing gums, chews : Better use them instead of snacking

Beverages and instant drinks : 0.088% of Satiereal® AWS per bottle of 200g gives a clear, pleasant and efficient drink. Formulations with Raspberry, Hibiscus, Apple juice, Cranberry, Iced tea, ... or even only water (yellow translucide solution). Other possible associations (additional claims) with for instance 500mg of Garcinia cambodgia extract standardized to HCA (« Weight management», also in beverages), 200mg of Fucus powder.

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Trust the Most Studied Curcuma Sabinsa has developed a reputation for being a trusted and valued resource to academic and medical communities in providing C3 Complex® for a variety of research initiatives. Feel quiet when using the Curcuma longa from the leading Cie

New Metabolic syndrome A significant study on Curcumin C3 Complex® (in combination with BioPerine®) in patients with metabolic syndrome has been published on end of 2014, showing the significantly greater effect of Curcuminoids in reducing the serum concentrations of LDL-C, non-HDL-C, total cholesterol, triglycerides and Lp(a), when serum HDL-C concentration was elevated significantly. Serum sdLDL levels were comparable (randomized, double-blind, placebo, 117 patients, 8 weeks).

New - Osteoarthritis Another study on 53 subjects for 8 weeks with C3 Complex and BioPerine shows that this combination brought a significant improvment in arthritic indices such as VAS (SVisual Analogue Scale), WOMAC (WOM Osteoarthritic Index) and LPFI (Pain Index).

abinsa’s Curcumin C3 Complex® enjoys a special place among curcumin extracts. C3 Complex is obtained from the dried rhizomes of Curcuma longa (turmeric) and standardized to min. 95% Curcuminoids. The name C3 Complex has reference to its three main chemical compounds – Curcumin (75-85% of the composition), Demethoxycurcumin and Bisdemethoxycurcumin – collectively known as Curcuminoids.

Traditional knowledge The rhizome of turmeric has a rich history in India as a spice, food preservative and coloring agent, and has been used for centuries in the Ayurvedic system of medicine : well-documented for treatment of various respiratory conditions (e.g. asthma, bronchial hyperactivity and various allergies), liver disorders, poor appetite, rheumatism and influenza symptoms.

Pharmacological activity Turmeric and more appropriately the components curcuminoids have been preclinically and/or clinically validate for their role in maintaining health and wellness. At the molecular level, the curcuminoids have been showed to inhibit nuclear factor kappaB (NGkB), a transcription factor that triggers inflammatory mediators. Curcumin C3 Complex counteract free radicals in 2 ways : à Prevention of free radical formation à Intervention whereby already preformed radicals are quenched by the curcuminoids. Curcuminoids offer antioxidant, anti inflammatory , and a healthy immune system support, and potential prevent connective tissue break down through inhibiting destructive enzymes, with benefits in healthy aging and all-related oxidative stress (including diabetes mellitus (and the secondary complications of the disease). Studies reported It protects the liver against several toxicants both in vitro and in vivo. Various other researches are conducted especially against metabolic syndrome (see below), as well as Alzheimer (anti-amyloid b), and cancers (breast, bladder, pancreatic, skin) where curcuminoids act by inhibiting several processes.

Why Curcumin C3 Complex stands out from the crowd ? à Most clinically studied Curcumin brand: more than 65 scientific publications, including clinical trials, have been published, and more than 100 articles. à Free curcuminoids only, and no inactive metabolites à Most active form (independent comparison of various Curcuma 95) à Most safety data conducted with C3 Complex and not another curcuma extract. Only Curcumin in the market that has been reviewed and acknowledged by US FDA for GRAS status, a process which includes a comprehensive review of safety and toxicological data à Most Patent-IP protection: Holds patent covering composition of curcuminoids, method of manufacture and uses à Most consistent quality: C3 Complex has the same composition of the three Curcuminoids consistently in the same ratio in every batch à It is known for its very low oxalic acid content (what caused kidney stones), heavy metal, ... à Most stable supply chain: C3 Complex is promptly delivered from European buffer stock à More proven bioavailability à Dedicated unit: C3 Complex is processed all over the year in its dedicated unit on a continuous batch extractor, without risk of cross contamination, from known fields (traceability) à Various awards à Grades available

Curcumin C3 Complex 95% Curcumin C3 Complex 95% DC (Direct Compression) Curcumin C3 Complex BWS (with Bacosides) Curcumin C3 Complex Beadlets Dispersible Curcumin C3 Power Liquid

Beverage-grade with pleasant taste now available Sabinsa introduce a new form of Curcumin which is new market-ready, product formulation concept, Curcumin C3 Power Liquid , which was the Tasting Center Award Winner at Vitafoods Asia 2019.

All form of curcuminoids are not equal. Some forms can be bioavailable, but unfortunatley inactive.

Sabinsa’s Curcumin C3 Dispersible - a standardized powder extract containing 60% Curcuminoids - effectively and efficiently resolves the solubility challenge of curcumin.

Developed specifically for RTD beverages and powdered drink mixes utilizing natural excipients as a base, it can be added to water or juice, or used in chewable/ dispersible tablets. It is not an instant powder, as it must be firstly dissolved. BECARRE Natural - October 2020 - 23

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wide array of human health benefits related to mental health and physical performance has been clinically demonstrated using the unique phytomedical form of R. rosea extract specifically standardized to rosavins. Rhodiola rosea possesses valuable anti-fatigue, anti-stress, and anti-depressant properties ; it stimulates the bioelectrical activity of the brain and improves memory and mental performance. In addition, it also increases stamina and accelerates the physical recovery processes after intense training workloads ; it stimulates muscle energy status, improves glycogen synthesis in the muscles and liver and increases muscle protein synthesis and anabolic activity.

The right product from the right place at the right time

Rhodiola: Memory, Immunity & Energy • Phenylpropanoid : Rosavins are products of the phenylpropanoid metabolism. Rosavins (ie. rosavin, rosin and rosarin) are specific to the root from Rhodiola rosaea. The rosavins are the constituents currently most often selected as the marker compounds for standardization of extracts, although they are not necessarily the only pharmacologically active ingredients for its medicinal properties. • Principal phenylethanoids consist of glycosides and salidroside has been reported as the most active tyrosol glycoside, being associated, together with rosavins, to the anti-depressive and anxiolytic effects (Maslowa, 1994; Tolonen, 2003). Other phenylethanoids have also been isolated but with today limited scientific literature concerning the bioactivity (Jiménez 1994).

While Rhodiola as a genus may have originated in the mountainous regions of Southwest China and the Himalayas, botanists have established that Rhodiola rosea naturally display a circumpolar distribution in mountainous regions in the higher latitudes and elevations of the Northern Hemisphere. In central and Northern Asia, the genus is distributed from the Altai Mountains across Mongolia into many parts of Siberia.

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Rhodiola rosea used for the production of RhodioLife® is wildcraft collected from this part, under the Russian Government License. Altai Mountains represents a pristine area free from contamination in of the most well preserved and remote natural environments. Nektium is involved from the early beginning in the collection practices, with SOPs describing the stage of the plant growth, best time of collection and ecologically non-destructive systems.

Active Ingredients Activities related to Rhodiola rosea have been traditionally attributed to the presence of four principal active ingredients: salidroside, rosin, rosavin and rosarin (Sokolov, 1985; Furmanowa, 1998). The root of R. rosea shows six distinct groups of chemical compounds. The Phenylalkanoids are the main contributors, incl. :

Attention and Memory Cerebral activity Rhodiola rosea is know to improve attention and cerebral performances.

All Rhodiola extracts are not equal Nektium is one of the very few Nutraceutical companies which has been granted an Export License by the Russian Government for the exportation of Rhodiola rosea roots from the Altai (Altay) region (Siberia). Nektium closely collaborates with its supplier implementing operating procedures that provide general technical guidance for the sustainable collection and processing of Rhodiola rosea roots, following the overall context of quality assurance and the WHO guidelines on Good Agricultural and Collection Practices (GACP). This includes the collection of the plants in the wild, as well as primary processing of the plant material, such as drying, packaging in bulk, storage and transport of the raw materials until it arrives to the Nektium factory. The impact of collection on the environment and ecological processes, and the welfare of local communities are also considered. Then the salidroside and the 3 actives rosavins only are controled (rosavin, rorin, rosarin) by Ultra HPLC, and no other molecules as rosiridin or tyrosol for instance. Grades are from 3% Rosavins (and related 1/3 salidroside), to 15%.

24 - BECARRE Natural - October 2020

Rhodiola rosea, also known historically as “Golden Root” from the “Golden Montains” (Mongolian word ‘Altan’), is the most popular phytomedicine traditional used to increase physical endurance, work productivity, longevity, memory and attention, resistance to high altitude sickness, fatigue, depression, and disorders of the nervous system.

On 2017, Nektium has conducted new studies: Electroencephalic studies (ECG) showed that animal supplemented with Rhodiolife® have an increased neurotransmitter production in the studied areas. This effect lasts at least for 5 hours until the end of measurements, and so even more. Besides of a stimulatory effect, the results indicate an improve of memory and an increased activity in brain regions related to motivation and reward.

R. Rosea increases the bioelectrical activity of the brain which improves memory and brain energy. Another study also described the Acetylcholine Esterase Inhibitors in Rhodiola rosea. It helps the body adapt to stress by affecting the levels and activity of serotonin, dopamine, and norepinephrine, neurotransmitters found in different structures in the brain and influencing the central nervous system. Rhodiola inhibits the breakdown of these chemicals and facilitates the neurotransmitter transport within the brain. In addition to its impact on the central nervous system, it increases the chemicals that provide energy to the muscle of the heart and prevent the depletion of adrenal hormones induced by acute stress. (Tori Hudson, 2006)

Immune System The Rhodiola extract Rhodiolife® has clearly demonstrated its ability to protect cells from viral infection. This is the first time shown to be effective in the activation of the human immune system (launch and human in-vivo study April 2015 Ahmed M, et al (2015) «Rhodiola rosea Exerts Antiviral Activity in Athletes Following a Competitive Marathon Race». Front Nutr. Jul 31;2:24.). It stimulates and protects the immune system (homeostasis), and increases the natural killer cells (NK) in the stomach and spleen. This action may be due to its ability to normalize hormones. Cardiovascular : Linked to the anti-stress action (and decrease of catecholamines and corticosteroids released by the adrenal glands during stress), R. rosea decreases the amount of cyclic-AMP (cAMP) released into cardiac cells, and so assist the uptake if lire intracellular calcium for greater heart muscle contraction (regular beat).

ATP & Energy The effect of Rhodiola rosea (RR) supplementation on ATP content in muscles was studied in Sprague-Dawley rats, where 24 adult rats were divided 3 groups equally: control group, RR (50mg/ Kg) and Rhodiola crenulata (50mg/kg). Two sessions of forced swimming with 30-min intervals were carried out every day. The decrease in ATP content in rats receiving R. rosea extract was statistically significantly less pronounced than the other groups (1). In a human trial, a total of 36 healthy untrained volunteers (2124 years of age) were randomly, equally divided in 3 groups (control, placebo and RR extract). Individuals in the placebo and RR extract groups received 2 capsules a day of each treatment (placebo or 340 mg of RR extract) for 30 days before and 6 days after exhausting physical exercise was performed (computer aided bicycle ergometer with gradual

Bacosides extracted from Bacopa moniera (Brahmi) can revitalize the intellectual functions (in children) and calm down the stressed mind, allowing for better brain function. In India, Bacopa monniera, known as «Brahmi», is revered in the indigenous system of medicine as a nerve tonic. The traditional use of the plant, of particular relevance to contemporary medicine, is its validated efficacy in promoting memory functions and providing relief to patients with anxiety neurosis. In Ayurveda, Brahmi is described as «medhya rasayana» or brain tonic with the ability to promote mental functioning along with providing general rejuvenative effects. In early literature, the name Brahmi was also used to refer to another plant species, Centella asiatica, but both are distinctly different.

increases of workload). Changes in biochemical markers of muscle damage and inflammation were significantly less pronounced in those taking RR supplementation. Rhodiola rosea balance the body’s stress-response system. Rhodiola extract one hour before exercise significantly increases capacity for endurance exercise. It also shortens recovery time after long workouts, to increase strength, and anti-toxic action. Rhodiola extract reduces levels of C-reactive protein, an inflammatory marker, and creatinine kinase, a marker of muscle damage (Abidov M & al., 2004). An other study from Nektium suggests that Rhodiola extract protects against peroxide-induced oxidative stress through the modulation of HPS70 (heat shock protein) in C2C12 muscles cells.

Response to Stress Rhodiola rosea balance the body’s stress-response system. Adaptogenic plants naturally increase the body’s non-specific resistance and normalise the functions of the body, with a more resourceful manner when facing stressful situation. R. rosea impacts central monoamine levels. R. rosea is said to assist the transport of serotonin precursors, tryptophan and 5-HTP into the brain. (Studies shows that serotonin brain neurotransmitter is involved in many functions such as pain perception, behavior, smooth muscle contraction, temperature regulation, appetite, etc... Case studies have reported that R. rosea helps with depressive syndromes, memory loss, anxiety, cognitive dysfunction and menopause related symptoms.

Oxidative stress

(HSP70) expression. «The aim of this study was to analyze the efficacy of a Rhodiola rosea extract (Rhodiolife) in protecting murine skeletal muscle cells (C2 C12 myotubes) from chemically induced oxidative stress and to establish whether modulation of HSP70 expression is observed. C2 C12 cells treated with Rhodiolife did not experience any loss of viability (p > 0.05) at concentrations of 1-100 µg/ mL for up to 24 h. In control cultures, viability decreased 25% following exposure to 2 mM H2 O2 (1 h). However, no significant decrease in viability in cells pre-treated with extract at concentrations as low as 1 µg/mL was observed. HSP70 mRNA levels were up-regulated two-fold in cell cultures treated with Rhodiolife (10 µg/mL), and expression was further enhanced by exposure to H2 O2 (six-fold, p < 0.05). HSP70 protein levels were maintained in pre-treated cell cultures compared to controls but was significantly lower (-50%) in cells lacking treatment exposed to H2 O2 . The present results indicate that Rhodiolife protects C2 C12 myotubes against peroxide-induced oxidative stress through the modulation of the molecular chaperone HSP70.». Hernández-Santana et al (2014), Phytother Res. 2014 Apr;28(4):623-8.

Anti-inflammatory Studies in healthy volunteers demonstrated that supplementation with Rhodiolife® has an anti-inflammatory and protective effect on muscle tissue during excercice. Abidov M, et al (2004), Bull Exp Biol Med. Jul;138(1):63-4.

ESSNA award: Best Targeted Sports Nutrition Product Doping free (BSCG certified) ID & DNA Testing

Rhodiola rosea root extract protects skeletal muscle cells against chemically induced oxidative stress by modulating heat shock protein 70

Bacopa revitalizes intellectual functions Chemical constituents The pharmacological effects of Bacopa monniera are attributed to the presence of a number of biologically active compounds, including alkaloids, saponins and sterols. The compounds responsible for the memory enhancing effects of are triterpenoid saponins called «bacosides» (Bacoside A3, bacopaside 1, bacopaside 2, jujubogenin, bacosaponine C).

Properties The biological effects of Bacopa monniera are well documented in traditional as well as scientific literature. The most important of these are the effects of the plant, plant extracts and isolated bacosides on cognition and memory functions, their anxiolytic effects and their role in the management of convulsive disorders. On receiving signals, receptors of the hippocampus trigger an electric pulse, mediated through a

change in protein composition The pulse is transmitted to the next neuron through the synapse. The process continue till the bond between neurons become strong and the memory is created However, continuous electrical activity wears out the synapses, impairing a new memory creation and causing loss of memory The bacosides help in restoring the synaptic activity of neurons. Bacopa is a cognitive enhancer in cases of : à Declining Memory à Poor Concentration à Attention Deficit Hyperactivity Disorder à Anti-convulsive or Anxiety Neurosis Dosage : 15 to 30 mg of Bacosides 3 times daily for 20% extract (dosage must be adapted to the grades 20% or 50%) BECARRE Natural - October 2020 - 25

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Bromelain / Papain

Natural proteolytic enzymes

romelain is a cysteine protease isolated from pineapple stem, Ananas comosus. Bromelain is a mixture of different thiol endopeptidases and other components like phosphatase, glucosidase, peroxidase, cellulase, escharase, and several protease inhibitors. In vitro and in vivo studies demonstrate that bromelain exhibits various fibrinolytic, antiedematous, antithrombotic, and anti-inflammatory activities.

Properties

Papain is well known for improving digestion and treating infections, diarrhea, and allergies. It has also been used, with other protease to reduce pain, inflammation, and swelling. It’s also being studied for potential use in cancer and other diseases.

Papain has shown antimicrobial activity against a number of microorganisms such as Bacillus subtilis, Enterobacter cloacae, E. coli, Salmonella Typhi, Staphylococcus aureus and Proteus vulgaris.

Effect of Bromelain on inflammatory

Digestion / Slimness

Bromelain have the ability to modulate surface adhesion molecules on Tcells, macrophages and natural killer cells and also induce the secretion of IL-1β, IL-6, TNFα by peripheral blood mononuclear cells. Bromelain can block Raf-A and ERK-2 pathways by inhibiting the T cell signal transduction. This induces the decrease of the activation of CD4 T cell and reduce the expression of CD25.

Papain is used to promote digestion: discomfort, ballooning. This use is based on in vivo studies of hydrolysis of different proteins in the presence of papain. This demonstrated a specificity of action of papain towards the peptide bonds containing lysine or arginine in acid media (pH 3-5).

Bromelain is a food supplement that may provide an alternative treatment to nonsteroidal antiinflammatory drug (NSAIDs). It plays an important role in the pathogenesis of arthritis. Bromelain has analgesic properties which are thought to be the result of its direct influence on pain mediators such as bradykinin.

Allergic airway disease Bromelain was found to attenuate development of allergic airway disease (AAD), while altering CD4+ to CD8+T lymphocyte populations. From this reduction in AAD outcomes it was suggested that bromelain may have similar effects in the treatment of human asthma and hypersensitivity disorders

Inflammatory bowel disease Investigation on bromelain’s anti-inflammatory action as a potential therapy for ulcerative colitis and Crohn’s disease, 2 types of inflammatory bowel disease. Bromelain discourages the release of inflammatory chemicals called cytokines and chemokines from diseased tissue (Test tube study ; Duke University gastroenterology).

Digestive Aid Bromelain is best known for its ability to break down proteins — making this enzyme a popular digestive aid. Bromelain has been used successfully as a digestive enzyme following pancreatectomy, in cases of exocrine pancreas insufficiency and in other intestinal disorders. Because of its wide pH range, bromelain has activity in the stomach as well as the small intestine. It has also been shown to be an adequate replacement for pepsin and trypsin in cases of deficiency. 26 - BECARRE Natural - October 2020

Bromelain and Papain are part of the family of proteolytic enzymes. They are systemic enzymes, designed to survive the acidity of the stomach and to go into the small intestine where they are absorbed into the bloodstream. There, they degrade proteins, thus exerting multiple beneficial effects for health, including platelet aggregation inhibition, digestive aid and anti-inflammatory effects.

Antimicrobial action

Its effects are mainly a product of its proteolytic activity, which stimulates fibrinolysis by increasing plasmin, but bromelain also has been shown to prevent kinin production and to inhibit platelet aggregation. It is also used for preventing muscle soreness after intense exercise, and for facilitate digestion.

Osteoarthritis

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apain, a papaya (Carica papaya) fruit latex extract, is a cysteine protease that catalyzes the cleavage of peptide bonds using a catalytic triad involving a deprotonated cysteine residue.

By diffusing in the body the papain would allow the fractionation of the abnormally secreted proteins, like the collagen fibers of the cellulite, and facilitate their elimination as well as that of the greasy cellulite.

Uses in association of systemic enzymes Accelerate recovery and healing of muscle damage Numerous studies in Europe show that supplementation with proteolytic enzymes effectively speeds up recovery after physical exercise and injuries in athletes. It also speeds up tissue repair in patients who have undergone surgery.

Bromelain Source: Ananas comosus - Stem Bio-activity: 1 200 to 5 000 GDU / g ≈ 2,4 to 10 FIP / mg Units correspondence: 1 FIP/mg ≈ 500 GDU/g

Papain Source: Carica papaya Bio-activity: 1,5 to 3,5 FIP / mg Units: 1 FIP ≈ 10 to 12 000 USP ≈ 160-180 TU / 100 TU ≈ 6 000 USP ≈ 0,6 FIP

Reasoned culture Krishna Enzytech cultivates in agreement with the farmers and also has their own farms in the coastal regions of Andhra Pradesh. Papaya is grown between pineapple plants, helping to manage water consumption, in soil maintained at a pH of 5.5 to 6.0. Once harvested, the fruits are sold to the fruit industry and the stem is dried and used for making bromelain.

In a study of footballers with ankle injuries, supplementation with proteolytic enzymes accelerated their healing and allowed players to return to the field 50% faster than placebo athletes. Several small trials in athletes have shown that enzymes can help reduce inflammation and accelerate healing of tissue lesions (including fractures) and reduce overall recovery time compared to placebo athletes.

A double-blind German study of dentist patients showed the anti-inflammatory effects of a mixture of 100 mg pancreatin, 45 mg bromelain, 60 mg papain, 10 mg lipase, 24 mg trypsin and 1 mg of chymotrypsin. Patients took this mixture before and after a dental operation for several days. Three days after the operation, levels of C-reactive protein, a marker of inflammation, were three times higher in the control group.

In patients undergoing various reconstructive and facial procedures, treatment with proteolytic enzymes significantly reduced swelling and pain compared to placebo patients

DigeZyme® is a unique proprietary blend of specific digestive enzymes that offers much more than just aiding proper digestion and better absorption of nutrients. DigeZyme® consists of five GMO-free broad acting enzymes obtained from the fermentation process: à Amylase : breaks down carbohydrates such as starch and glycogen, into smaller units à Protease: Breaks down protein and supports immune function à Lactase: Breaks down lactose (milk sugar) and useful for lactose intolerance à Cellulase: Breaks down cellulose and chitin. It helps free nutrients in both fruits and vegetables à Lipase: Breaks down lipids and improves fat utilization and also supports the most healthy gallbladder function

Inflammation The use of systemic enzymes is particularly effective during inflammation because they hydrolyze the peptide bonds that unite amino acids to proteins, thus dissolving clumps of fibrin. This reduction in fibrin allows more oxygen to reach and reactivate the tissue cells. Excess fluid is also reabsorbed, reducing inflammation.

Other enzymes

Nigellin - Elixir for Overall Health and Wellness Sabinsa’s Nigellin™ is obtained from the seeds of Nigella sativa (Black Cumin) and standardized to contain 5% Thymoquinone

abinsa’s Nigellin represents patent pending, solvent-free, supercritical fluid extraction (SCFE) black cumin seed extracts, standardized to contain thymoquinone. Nigellin is available as oil (Nigellin BCS - Amber), and defatted black and white powders (Nigellin BCS - Onyx and Nigellin BCS - Pearl, respectively). Nigella sativa, popularly known as ‘black seed,’ or ‘black cumin,’ one the most revered medicinal seeds in history of mankind, is indeed a gift from the Mother Nature— owing to its well-established, sanctified, and miraculous uses for over 3,000 years. Various medicinal and healing properties of black seed are directly mentioned in the Qur’an as ‘blessed food.’ Black seed has also been mentioned in the Bible as the ‘curative,’ used by Asian herbalists and the Romans for culinary purposes for centuries. Thus, it is not surprising that Prophet Muhammad had praised black cumin by saying that “Hold on to the use of the black seed for indeed it has a remedy for every disease except death.”

Chemical constituents

One of the reasons for considering black seeds as the main sources of nutrition and healthcare might be due to the complex chemical structure of the seeds. These little seeds contain more than 100 different chemical constituents, including carbohydrates, proteins, volatile and fixed oils, and are also an abundant source of all the essential fatty acids. Thymoquinone is proved to be the main active constituent of the volatile oil of the black seed and is known to have several medicinal properties.

Ellagic Acid is traditionally extracted from Pomegranate or Terminalia. Ellagic acid is kown as skin lightening and brightening, repairing from sun damage, besides strong antioxidant properties and skin elasticity improver Ellagic acid is used in topical formulation for its lightening effect on UV induced hyperpigmentation. It’s know that Ellagic inhibits melanin forma-

A timehonored remedy Though black seed has been recognized as one of the most popular herbs in many parts of the world for centuries, extensive research during last 40 years has further validated its potential health benefits, viz. maintain healthy inflammatory response (Mahdavi et al., 2016), asthma control support (Koshak et al., 2017), improved glycemic control in diabetic patients (Bamosa et al., 2010), protective role in diabetic nephropathy (Ansari et al., 2017), healthy blood pressure (Dehkordi and Kamkhah, 2008) and lipid profile management (Sabzghabaee et al., 2012), helps boost male fertility (Marbat et al., 2013; Kolahdooz et al., 2014).

Asthma

Recent research published in Frontiers of Pharmacology identified Nigellin™ BCS - Amber, Sabinsa’s Black Cumin (Nigella sativa) seed extract, as being the most effective of the 10 Nigella sativa (NS) extracts used in an in vitro study which suppressed the release of the most active cytokines and identified a prostanoid with inflammatory properties which may possess a favorable effect in the context of asthmatic conditions (Comparative Im-

munomodulatory Activity of Nigella sativa L. Preparations on Proinflammatory Mediators: A Focus on Asthma, Front.

Pharmacol., 02 October 2018). Researchers evaluated 10 different NS extracts, and top ranked the extract provided by Sabinsa’s parent company and manufacturing arm, Sami Labs. It surpassed the second ranking extract in terms of the active constituent, thymoquinone (TQ), by more than 300%. Anti-inflammatory and immunomodulating properties are among the most valued in anti-asthma herbal products. Nigellin had the highest effects in suppressing the release

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of cytokines IL-2, IL-6 and PGE2 in T-lymphocytes and also in monocytes hindering the expression on IL-2 and PGE2. These are among the most active cytokines involved in asthmatic conditions. The investigators noted a positive relationship between the TQ level in the preparations and its inhibitory potency on IL-6 and PGE2. However, PGE2 does exhibit beneficial bronchodilatory effects in human lung epithelial cells and Sabinsa’s extract showed an enhanced PGE2 expression in A549 human epithelial cells lines, demonstrating an interesting activity behavior of the maximum-TQ containing Sabinsa’s extract. The results presented in this paper formed the biochemical basis of a subsequently conducted clinical trial. Asthma is a debilitating disease afflicting 25 million people in the USA, alone and 334 million globally according to some estimates. It tends to flare up sharply due to external factors such as pollution and allergens. Nigella sativa and its preparations have been referenced extensively in traditional healthcare systems ranging from Ayurvedic and Unani to Arabic and Prophetic for prevention and treatment of asthma, making it a candidate for study by modern researchers. Lack of standardization has, however, hampered researchers in evaluating the material’s efficacy in the past. “This emerging antioxidant ingredient has traditionally been used in the management of numerous health conditions, with recent research beginning to validate historically reported benefits,”. Elixir for Overall Health and Wellness

Oral Ellagic Acid for Skin Lightening tion by acting on tyrosinase. Oral Administration of Ellagic Acid for skin lightening Oral administration of ellagic acid (alcoholic extraction followed by acid hydrolysis) has been studied, and its metabolites have been detected in urines and feces. A whitening effect on UV-induced pigmentation was observed, with a reduction on the number of DOPA-positive melanocytes (Mineka Yoshimura, 2006). A double blind vs placebo clinical trials has evaluated the protective and ameliorative effect at 200mg and 100mg of ellagic acid per day, orally, followed by UV radiations. It’s so suggested that Ellagic acid orally administrated is absorbed into the body and the ellagic

and/or its metabolites inhibits the proliferation of melanocytes in the skin. Those studies have been performed in Japan, with products offered by Sabinsa Japan Corp. Effects or Oral Administration of Ellagic Acid-rich pomegranate Extracts on UV induced pigmentation in the human skin, Kouichi Kasai, Mineka Yoshimura, Takuro Koga, Masayuki Arii, Satoru Kawasaki, J Nutr Sci Vitaminol 52, March 2006. Inhibitory Effect of an Ellagic Acid-rich Pomegranate Extract on Tyrosinase Activity and UV-Indudex pigmentation, Mineka Yoshimura, Yuko Tanabe, Kouichi Kasai, Yuan Yamakoshi, Taguro Koga, Biosci. Biotechnol. Biochem 69, 2005 BECARRE Natural - October 2020 - 27

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The Effective SOD for Health & Skin

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Vouldoukis et al. have roduction of reThanks to its gliadin matrix, shown ex vivo that active oxygen GliSODin® is orally effective prime activation of macspecies (ROS) is unlike most existing SOD rophages isolated from a normal process supplements. rodents with interferonin oxygen-breathing orThe gliadin is not only an enteric gamma (INF-gamma) ganisms. Under normal protection but more : a real subsequently challenged physiological conditions a balance between these vehicle for the SOD, thanks to its with IgGl/anti-IgG1 imspecies and the body’s specific bio-adhesive properties mune complexes leads to the significant proanti-oxidant defenses with the epithelial cells in the duction of superoxide exists. However, certain small intestine. anions. This production conditions can increase the production of Reactive Oxygen Species may be regulated, in a dose-dependent man(ROS) such as the superoxide ion (O2-) and ner, in macrophages originating from rodents previously supplemented with GliSODin®. the hydroxyl ion (OH-).This can disrupt the natural balance and lead ultimately to oxida- These results prove the potent in vivo activation of antioxidant activities made by the tive stress. SOD-containing melon extract/gliadin biopolymer combination. Cells defend themselves? An important proof of concept in vivo study Superoxide dismutase (SOD), along with catby Vouldoukis et al. using Balb/c mice receivalase and glutathione peroxidase, form the ing the SOD melon extract orally, either alone front line of the body’s antioxidant enzyme or combined with wheat gliadin (GliSODin®) defenses[4].The superoxide anion is the startfor 28 weeks showed that only the gliadining point of the cascade of reactions of free SOD complex resulted in a significant inradical production. crease in circulating antioxidant levels. SOD, dubbed the “enzyme of life” on discovAt the end of the study, the animals suppleery in 1968, is the first antioxidant mobilized mented with GliSODin® had significantly by the cell as defense against oxidative stress. lower levels of oxidative-stress induced DNA The enzyme reacts with the superoxide ion damage. Furthermore, the researchers found and turns it into hydrogen peroxide (H2O2). lower levels of apoptotic cells in the spinal This is then cataboliwzed by catalase and fluid, thus showing a marked protective benglutathione peroxidase to produce molecular efit. oxygen (O2) and water (H2O). These antioxidant enzymes have a distinct advantage over the antioxidants consumed Oxidative stress & from the diet or nutritional supplements, DNA damages like the vitamins A, C, and E, carotenoids, GliSODin®’s protective benefits have been and thiols since the enzymes are biological demonstrated in human interventional studcatalysts, rapidly and repeatedly reducing ies. GliSODin® was shown to protect against reactive oxygen species without being concellular oxidative stress damage in a dramatic sumed themselves. Like most other protechuman model. In this double-blind, placebotive mechanisms in the body, the production controlled trial, twenty healthy volunteers of SOD decreases with age[5], while a cell’s were given pure oxygen in a hyperbaric susceptibility to oxidants increases, putting chamber (HBO), which increased atmospherthe cells under increasing oxidative stress. ic pressure to 2.5 times normal, inducing intense oxidative stress. A measure of blood Oral administration cell protection, looking at the integrity of the cellular DNA, was taken before and after Oral administration of SOD and the other antioxidant enzymes present in many plant exposure to measure the effect of oxidative stress. The GliSODin® group had significantly extracts is, under normal conditions, not eflower cellular DNA damage as evidenced by a fective. During passage through the gastrointestinal pathway the enzyme is denatured test called “Comet Assay.” Also, these findings coincided with reduced blood isoprostane (deactivated) rendering it ineffective as an levels, another marker of oxidative stress. antioxidant. However, studies have shown that combining SOD with a wheat gliadin In the GliSODin group, the cell nucleus is intact, while the placebo group shows probiopolymer system temporarily protects the SOD during its passage through the gastro- gressive nucleus damage with pronounced “comet tail”. intestinal tract. One explanation of this efficiency was presented by Clemente et al. www.glisodin.org & www.glisodin.org who showed that gliadin increases the permeability of the intestinal wall by promoting The enzymatic activity of oral free SOD the release of a zonulin, thereby allowing the in the body is mainly to totally detransport of the macromolecule SOD across stroyed within few minutes, despite the the intestinal barrier. source, and can so not promote the proThe combination of SOD extracted from duction of the SOD cascade by the cells cantaloupe melon (Cucumis melo L.C.) with and the related expected optimal activithe wheat gliadin biopolymer (GliSODin®) ties. For this reason, usual studies based significantly improves the delayed release of on free SOD cannot prove the efficacy, SOD as evidenced in vitro by the progressive or at an incredibly high use (in-vivo). increase of its activity in a medium mimicking Dose : 2 x 100mg to 2 x 250mg a day the digestive conditions . 28 - BECARRE Natural - October 2020

First orally effective SOD, GliSODin® is the new oral active for skin health, immune system and free radicals protection supported by more than 25 mostly published studies • GliSODin® is both a protected and targeted SOD, supported by more than 25 clinical and scientific studies. • GliSODin®, a patented nutraceutical ingredient, has been shown in clinical research to : • Promote the production of the body’s own, natural antioxidants • Help maintain cellular health and protect against damage caused by oxidative stress • Inhibition of endometriosis extension (mice) • Support skin health against photo-oxidative stress • Reduce lactic acid buildup under physical stress • Support healthy immune function • Promote cardiovascular health • Prevent stress induced impairment of spatial memory (neuroprotection)

Anti-aging • • • • • • • •

Protects against DNA damage Prevents oxidative stress Decreases troubles induced by ageing Improves cognitive functions Protects against photo aging Supports healthy immune function Reduces risk factors of cardiovascular disease Helps prevent light sun allergies

sis, which is a major risk factor for cardiovascular disease. GliSODin® promoted arterial health and function compared to the control group as measured by the thickness of the participants’ carotid arteries. Further, GliSODin® significantly improved the antioxidant status and provided a reduction in measures of lipid oxidation. The researchers called the results «remarkable”. (Naito et al.) No significant differences in blood glucose levels or body weight were observed between the diabetic mice in the SOD-gliadin- supplemented group and the control group. In terms of kidney health, significant reductions in the levels of 8- hydroxydeoxyguanosin (8-OHdG), a marker of oxidative stress, had been observed in the SOD-gliadin-supplemented group.

Sun & skin health • • • • •

Increases the MED (Minimal Erythema Dose) Helps prevent light sun allergies Prepares the skin for sun exposure Reduces sunburn redness Faster recovery after sunburn and photooxidative stress

Sport & inflammation

A randomized double-blind clinical trial by Mac-Mary et al. showed that GliSODin® supplementation reduced skin-reddening when healthy fair-skinned volunteers were exposed to UV radiation. Fifty subjects were randomly assigned to receive a daily dose of GliSODin® (500 mg) or placebo four weeks. Subjects were exposed to UV radiation to induce sunburn on the innerforearms, and the susceptibility of the participants to sunburn (defined as the minimum erythematous dose – MED) and a measure of the resulting redness (actinic erythema). Supplementation with GliSODin® results in an increase in the minimum exposure to UV rays necessary to produce skin burn for fair-skinned people (phenotype II), compared to placebo. The induced redness also decreased quicker in the GliSODin®-supplemented group over the four-week period. These results confirmed the efficacy of the SOD-gliadin combination against the consequences of oxidative stress produced by exposure to UV radiation. In a later study of similar design with Type II (fair-skinned) participates, GliSODin significantly increased the MED in two weeks’ time with a dose of just 250mg.

GliSODin® was studied for its ability to regulate the immune response. Supplementation with GliSODin® had increased production of type1 helper T lymphocytes (Th1) and INF-a and IL-4.The immunoglobulin G (IgG) response – the predominant antibody used by the body to identify and neutralize foreign objects, while the response of IgE, the immunoglobulin associated with an allergic response was only marginally affected. Vouldoukis et al. proposed that the mechanism behind these effects was due to an activation of antigen presenting cells (APC), which results in the production of hydrogen peroxide (H2O2) and nitric oxide (NO), both of which are reactive oxygen species and upset the oxidant-antioxidant balance. In response to this, production of the antioxidant enzymes catalase and glutathione peroxidase is induced. This results in a polarized adaptive immune system, highlighting the benefits of GliSODin® since this polarization is a sign of the natural equilibrium of antioxidants in the cells. Rahman et al. investigated the effect of receiving GliSODin®-containing supplements on quality of life and performance of 23 AIDS patients on HAART (significant benefits for the millions of people infected with the HIV-1 virus and receiving anti-retroviral therapy).

Cardiovascular health • Reduces risk factors of cardiovascular disease • Decreases carotid Intima-Media thickness (IMT) • Prevents metabolic syndromerelated diseases

Immunity • • • • •

Supports healthy immune function Immune-modulating properties shown to be effective in children Acts as immune-modulator Regulates pro- and anti inflammatory cytokines production Induction of Th-1 immune response

New published study with GliSODin®: in the present randomized, placebo-controlled, double-blind clinical trial, the clinical efficacy of GliSODin® in accelerating symptom relief in children with allergic asthma and house dust mite allergy (receiving house dust mite immunotherapy) was demonstrated. In summary, GliSODin® accelerates the symptom relief in asthmatic children allergic to house dust mite receiving house dust mite immunotherapy. For the first time GliSODin’s immune-modulating properties were shown to be effective in children!

A published, three-year study with GliSODin® supplementation showed significant benefits for cardiovascular health. Using ultrasound-B imaging to measure carotid artery intima thickness (IMT), a sign of hardening of the arteries (atherosclerosis), the researchers found a reduction in the progression of IMT in the SOD-supplemented group, compared to the group with the prescribed diet and lifestyle. This study showed that supplementation with GliSODin® could impact the antioxidant status and the inflammatory process. The study also showed clear benefits against atherosclero-

• Anti-inflammatory effect: control C-Reactive Protein • Decreases in-exercise lactate production • Supports post-exercise immune function • Improves recovery after strenuous exercise

In an in vivo study, Vouldoukis et al. compared the effects on production of pro- and anti-inflammatory cytokines with SOD, Gliadine, heat inactivated GliSODin® and GliSODin®. Only GliSODin® reduced the production of the pro-inflammatory cytokine, tumor necrosis factor-alpha (TNFa) and promoted production of the anti-inflammatory cytokine interleukin-10 (IL-10), compared to the other treatments. The anti-inflammatory effects of GliSODin® are significant since chronic inflammation is associated with the onset and progression of many chronic diseases. Okada et al. reported that administration of the gliadinSOD complex could prevent cancer progression, promoted by inflammation. This result indicated that the antioxidant and anti-inflammatory properties of GliSODin® might have significant benefits for the prevention of tumor development and progression. A double-blind study included 19 participants who were participating in a training camp (2 capsules (total 500 mg) of GliSODin®). SOD activity was significantly higher (p <0.01) in the supplemented group at all measurement times, and post-exercise C-reactive protein was significantly lower in athletes receiving GliSODin®. Supplementation with an extract rich in SOD activity promoted antioxidant status and protected against increased inflammation in the serum of professional rowers. Hong et al. recruited 44 healthy individuals and assigned them to receive a daily GliSODin® dose of 1500 IU for four weeks. Healthy volunteers were then submitted to cycling or treadmill exercise but assigned into two distinctive groups: the severe exercise group (27 subjects) or the moderate exercise group (17 subjects). Only volunteers ranging from the severe exercise group showed a significant reduction in the exercise-induced lactate production, after the four-weeks of SOD supplementation.

BECARRE Natural - October 2020 - 29

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enXtra® Supported by human clinical trials Pilot Study : Human Safety & Efficacy 300 mg of enXtra® Alpinia galanga demonstrated optimal improvement in alertness for up to 5 hours when compared to other extract groups (70 caffeine habituated subjects) Second Human Study Randomized, double blind, double dummy, placebo controlled crossover study design : 53 caffeine habituated individuals with 4 study arms à Placebo à Caffeine (200mg) à enXtra® (300 mg) à enXtra® (300 mg) + Caffeine (200mg) 103,99%

22,70% 33,79% -23,12% -57,92%

-33,25%

-9,00% -18,01% -24,41%

-2,90%

19,76%

19,27%

-5,85% -1,17%

-25,89% -11,35%

-11,38% -11,08%

GG1

GG2

27,30%

Standard assessment tools : à Attention NetworkTest à Psychomotor Vigilance Task à Karolinska Sleep Scale score 1 hour

3 hours

5 hours

6,37%

% CHANGE ERROR RATE VS BASELINE

It brings a mild and pleasant taste

• The combination of caffeine with enXtra® significantly reduced mean response time as compared to placebo • Improved ability to achieve and maintain high arousal levels to better process new information. • The newly published study (2020) re-demonstrate the efficacy and the safety for a period of 12 weeks

-36,38%

• Maximum increase in alertness from baseline was observed at 3 hour interval

-17,40%

-11,78%

Study Findings

FOCUS HINTS

AG2

PLACEBO

CAFFEINE

-29,46%

5 hours

Assessments were taken at 1-hour, 3-hour and 5-hour time intervals

It does not increase heart rate or blood pressure and is safe & effective for 5 hours without the caffeine ‘crash’. enXtra® is water soluble and can be formulated with or without caffeine

3 hours

-14,00%

5,25%

enXtra® is a galangal extract that delivers fast, effective and safe results for improving mental alertness & acuity

PLACEBO CAFFEINE ENXTRA

0,00%

1 hour

CHANGE IN ALERTNESS SCORE

-52,84%

nXtra® from Alpinia galanga is a DNAauthenticated, stim-free enegry ingredient that provides fast, effective and safe energy with no crash. In a 53-person, double-blind, double-dummy human clinical trial, It was shown to improve mental alertness & acuity for up to 5 hours without increasing heart rate or blood pressure. It can be used as a replacement for caffeine or used with caffeine to prevent crash and prolong benefits. enXtra® is easy to formulate with, water soluble and has a pleasant taste profile.

12,41%

107,31%

Clinically proven to improve alertness and focus up to 5 hours with and without caffeine

ENXTRA 300MG

Molecular Docking Studies To better understand how enXtra® provides 5hrs of Mental Alertness & Sustain attention, without the crash, a molecular docking study of a database of molecules of enXtra® was performed against a panel of target proteins responsible

Starmeric™ The unexplored non-polar components of turmeric also have potential health benefits. Starmeric™ is a resistant starch, isolated from turmeric. 30 - BECARRE Natural - October 2020

The double-helical crystalline structure of resistant starch cannot be hydrolyzed by enzymes in the small intestine and it is passed into the large intestine for fermentation by gut microflora, there it produces short-chain fatty acids. The resistant starch, with the virtue of its slow degradability by the enteric enzymes, lowers the postprandial glucose level and can also improve the insulin sensitivity (Sun et al., 2018). When taken orally, short-chain fatty acids released during resistant starch metabolism by gut microflora

Sharpens alertness and focus for up to 5 hours No increase in blood pressure or heart rate Amplifies caffeine benefits without the “crash” Dopamine release AchE (Acetylcholine esterase) inhibition for enhancing attention network components; Dopamine (DA) is the primary neurotransmitter responsible for many physiological processes including alertness, focus, feeling of well-being and improved productivity. Dopamine reuptake through the plasma membrane protein, Dopamine transporter (DAT), is the primary mechanism for regulating dopamine concentration suite. Total 43 compounds, 25 belonging to EnXtra, were subjected to docking. As evident by glide score, active compounds of enXtra® demonstrated Glide score better than caffeine and increased dopamine levels by blocking its uptake. This increase in dopamine levels can enhance visuo-spatial performance and mental clarity, leading to improved mental alertness and better focus. In the current study, several compounds of enXtra® namely exhibited significant Glide score for AchE (Acetylcholine esterase) target enzyme, demonstrating their potential to inhibit AchE. Hence it can be concluded that bioactive molecules from enXtra® decrease dopamine uptake and inhibit the AchE target protein which leads to attention-enhancing effect and better understanding

Manufacturing Gentle, solvent free extraction for optimum bioactives. DNA match authentic galangal.

Responsible Sourcing

à Skillful hands cultivating galanga for more than three generations à Grown in hilly terrain away from pollution à Cultivated without pesticides à Hand picked at optimal daytime temperature à Natural drying on high altitude, stony land near farm

have stronger anti-inflammatory activity (Vino lo et al., 2011 ). Starmeric™ has been shown to possess anti-rheumatoid activity against Mycobacterium tuberculosis induced adjuvant arthritis. Additionally, various studies have shown that the resistant starch can improve serum lipid profile, improve colon health, improve gut microbiome, and can act as a potent prebiotic (Janine et al., 2004).

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Two Ways Anti-Inflammatory Boswellin® One of the most famous and powerful anti-inflammatory. Boswellic acids - and especially AKBBA - have been found to inhibit 5-lipoxygenase by one of two ways (direct interaction with 5-LO and Interacting with 5-LO activating proteins FLAP) as well as human leukocyte elastase (HLE).

nflammation is a complex process involving a series of actions and/or reactions and a broad range of biologically active substances (e.g. bradykinins, histamines, prostaglandins, thromboxanes, hydroxy-fatty acids, leukotrienes, lysosomal enzymes, and lymphokines) triggered by the body’s immunological response to tissue damage. Leukotrienes, important mediators in inflammatory and allergic processes, are produced from arachidonic acid, an essential fatty acid synthesized in the body, via the key enzyme 5-lipoxygenase (5-LO). Earlier work identified the boswellic acids (BAs) as specific, non-redox inhibitors of 5-LO. Thus, they inhibit the production of inflammatory leukotrienes. Based on IC50 (effective inhibitory concentration of tested substance) values, acetyl-11-ketob-boswellic acid alone provided the most potent inhibitory action due to its optimal structure.

Dual Inhibitory Action

What effective Boswellic Acid ? Boswellia extracts contain Boswellic acids. Grades of Boswellin are not titled as «total boswellic acid» - which means nothing since all acids would be included. It’s important to see the real content of the actives acids : the b boswellic acids (by HPLC) and more specifically the more potent Acetyl-11-keto-b-boswellic

Boswellin® PS Boswellin PS from Sabinsa represents an improvement over the existing conventional Boswellia serrata extracts, providing manufacturers with a more water soluble version with enhanced joint health support potential. Boswellin® PS offers a unique release profile for the active ingredients. In addition to boswellic acids, the PS version contains Polysal™, a natural polysaccharides fraction from the gum resin of B. serrata. The Polysal fraction is water soluble, and enhances the healthful role in the extract.

Boswellia extract inhibit 5-LO by one of two ways: à Directly interacting with 5-LO à Interacting with the five-lipoxygenaseactivating protein (FLAP). as well as, human leukocyte elastase (HLE), the serine protease produced and released by polymorphonuclear leukocytes (PMNLs) which has been suggested to play a role in several diseases (pulmonary emphysema, cystic fibrosis, chronic bronchitis, acute respiratory distress syndrome, glomerulonephritis, and rheumatic arthritis) due to its aggressive and destructive properties. This dual inhibitory action of two pathophysiologically important enzyme activities (HLE and 5-LO) is unique to the pentacyclic triterpenes from the boswellic acid series. Several leukotriene biosynthesis inhibitors furnished no HLE inhibitory activity : b-Boswellic acid, AKBA, ursolic acid, and amyrin significantly inhibited HLE. The HLE inhibition activity of AKBA and ursolic acid were comparable, however; ursolic acid’s mode of inhibition is competitive.

teoarthritisTotal 175 Subjects, 1-6 years duration, 10-50 yearsGupta, V.N, Yadav, D.S., Jain, M.P., Atal, C.K Results : 67% Good & Excellent, 30% Fair, 3% Poor Inflammation and its Relationship to Glycosaminoglycans à Glycosaminoglycans in the body form a ground substance found in connective tissue, mucous secretions and synovial fluids. à In the process of chronic inflammation of connective tissue, as exemplified by autoimmunological disorders, e.g. lupus erythematosus or rheumatoid arthritis, one of the first casualties of the destructive process of inflammation is the wasting away of the ground substance that is an integral element of the connective tissue. Thus the wasting away of the glycosamino-glycans in the joints leads to continuously worsening joint disfigurement and limited mobility Apoptosis Induction by AKBA Apoptosis or programmed cell death (PCD) is an essential process in normal animal development and in the renewal of cell populations in the human body. The effects of AKBA were studied in two human cancer cell lines, HL-60 and CCRF-CEM. AKBA reduced the viability and proliferation of the leukemic cells from both lines in a dose-dependent manner.

Antitumor Activity Shao and coworkers examined the antitumor activity of the four major pentacyclic triterpene acids of Boswellia serrata. All the compounds exhibited inhibitory activity in a dose-dependent manner. Based on cell morphology and NBT (nitroblue tetrazolium) reduction, BC-4 induced monocytic differentiation in myeloid leukemia cells in all three cell lines. Clinical studies on Malignant Glioma, Ulcerative Colitis and Bronchial Asthma were also performed.

Recommended dosage Oral : 75 to 200mg of extract - 2-3 times a day

Preclinical, in-vitro & Clinical studies Anti-inflammatory Effects Wildfeurer and coworkers studied Boswellia serrata as a leukotriene synthesis inhibitor of intact human PMNLs and as an herbal medicine for guinea pigs suffering from experimental autoimmune encephalomyelitis (EAE). Mixed acetyl boswellic acids significantly inhibited the ionophore-stimulated release of leukotrienes LTB.

Topical : 3% to 5% As it acts as a strong anti-inflammatory agent, BS extract may be used in stand alone or in combination with COX-2 inhibitors or nutrient enhancer (BioPerine®).

Study of Rheumatoid Arthritis (Clinical) Conditions : Rheumatoid Arthritis, Ankylosing spondylitis, OsBECARRE Natural - October 2020 - 31

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All Benefits from Pomegranate

he pomegranate (Punica granatum) is a fruit-bearing shrub or small tree that has been cultivated over the whole Mediterranean region and the Caucasus since ancient times. In the Northern Hemisphere, the fruit is typically in season from September to January while in the Southern Hemisphere, the season runs from March to May. The name itself derives from the Latin pomum (apple) and granatus (seeded) (Lloyd, 1897). There is a great interest in the scientific community in the properties of pomegranate, which is considered by many as a functional food because it has valuable compounds in different parts of the fruit displaying beneficial effects on health such as antioxidant, antitumoral , antihepatotoxic , antimicrobial, anti-inflammatory, antiviral, antidiabetic and improving cardiovascular, oral, and skin health. However, few well-controlled clinical trials have been completed and these effects have not been solidly established (ViudaMartos, 2010).

Pomegranate P40P

FOCUS HINTS

P40p™ is a unique product, carrier free, soluble in water and is standardized to a full spectrum of all of the essential compounds responsible for specific health promoting benefits of pomegranate juice, manufactured under strict GMP procedures. Composition

P40P

Total Punicosides (HPLC)

≥ 40%

Punicalagins A & B (HPLC)

≥ 30%

Total Polyphenols Folin Denis

≥ 50%

The P40p™ extract is standardized to 40% punicosides with a minimum concentration of 30% punicalagins. Additionally, P40p™ guarantees a total polyphenol content of no less than 50% (Table 1). The final product is free of yeast and bacterial contamination, allergens, gluten, BSE, GMO and irradiated products. P40p™ is Kosher certified and suitable for vegans.

Approximately, 30 mg of P40p™ provides an equivalent amount of bioactives than 50 ml of pomegranate juice.

Chemical Profile The key bioactive compounds in pomegranate fruit are a group of hydrolizable ellagitanins generically called punicosides, including punicalins, punicalagins (A+B), ellagic acid glycoside and ellagic acid. Ellagic acid is commonly used to standardize commercial pomegranate extracts but this approach is unreliable, potentially misleading and vulnerable to adulteration (Zhang, 2009). Punicalagins have been identified as being responsible for most of the antioxidant activity present in the pomegranate fruit (Gil, 2000) and represent a more reliable means of standardization of commercially produced pomegranate extracts (Jimenez Del Rio, 2006). Animal studies have shown that both ellagic acid and punicalagins are absorbed by the gastrointestinal track after oral ingestion of pomegranate juice or extract, with the concentration of the latter being higher. Punicalagins are not absorbed in vivo directly but reach the colon and release EA that is metabolised by the human microflora (Larrosa, 2006).

Cardiovascular Health Atherosclerosis is a collection of patchy plaques (called atheromas) in medium-sized and large arteries. These plaques contain lipids, inflammatory cells, smooth muscle cells, and connective tissue. Several investigators have studied the influence on cardiovascular health of pomegranate supplementation. They have measured how pomegranate affects several markers involved in the development of atherosclerosis, such as plasma lipid profiles, lipid-oxidation status, platelet aggregation, interaction between macrophages lipids, and stress to the arterial wall. More recently Rosemblat has also confirmed that pomegranate juice daily consumption improves plasma lipid oxidant profile in patients with diabetes by reducing lipid peroxide and cellular peroxides and increasing glutathione levels, factors that all contribute to development of atherosclerosis in these patients (Rosemblat, 2006).

Cancer Nektium has been extracting Pomegranate in their GMP Silliker plant located at Las Palmas for years. The most studied grades : à Pomegranate P40P brings bioactives compounds as punicosides and punicalagins A+B (strong antioxidant), and other polyphenols, carrier free : cardiovascular, antioxidant. Also some research on prostate health and joints. à Pomegranate seed oil for body weight and antioxidant, contains Punicic acid, fatty CLNA. 32 - BECARRE Natural - October 2020

Nektium offers a range of Pomegranate extracts looking to preserve the totum of the fruits, as in the famous P40P, and not only ellagic acids which is finally in a very small amount in the fruits. From the seeds, they extract punicic acids, studied then for healthy body weight.

Pomegranate Seed Oil Pomegranate seed oil (PSO) is rich in Punic Acid (PA) and contains other actives such as ellagic acid, other fatty acids and sterols (Schubert, 1999; Amakura, 2000; Abd El Wahab, 1999). Nektium manufactures PSO with at least 80% content of PA.

Punicic acid Punicic Acid (PA) is structurally related to Conjugated Linolenic Acid (CLNA), and their potential beneficial biological effects (for instance significant decrease in hepatic tryacylglycerol deposits, as well as levels of monounsaturated fatty acids). In fact PA has been found in animal models to accumulate in liver and perirenal fat, increasing serum levels of tryacylglycerol and phospholipids, which suggest PA mobilizes fat out of their storage sites into the blood (Yamasaki, 2006), corroborated later on in ICR CD-1 mice. At the molecular level, investigators have found that PA activates a class of receptors (PPARγ) also activated by oral antidiabetic agents, which in principle could play an important role in glucose homeostasis and obesity related inflammation.

Cancer is an unregulated proliferation of cells which lose their normal controls, resulting in unregulated growth, lack of differentiation, local tissue invasion, and, often, metastasis. Dozens of information (angiogenesis, apoptosis, cells differentiation and signaling) gathered on cellular cultures and animal models have been collected.

In summary, PSO has shown beneficial effects on the lipid storage and lipid serum profile in obesity animal models with a strong suggestion that it may also help uncontrolled blood glucose levels.

Pantuck et al found that daily supplementation with pomegranate juice (PJ) led to a significant decrease in tumor progression in patients with prostate cancer that had undergone surgery or radiotherapy. They were able also to confirm in in vitro investigations that PJ induced apoptosis and decreased cell proliferation (Pantuck, 2006)

PSO has been used in a formulation intended for weight management, together with fucoxanthin. In a clinical trial, investigators found that PSO acted synergistically with a fucoxanthin, increasing the resting energy expenditure (REE). Increase in REE leads to losing weight (Abidov, 2010). More recently, investigators have reported that PSO can down-regulate the expression of adipogenic genes (PPARγ) in fat cells.

Pomegranate Seed Oil in Weight Management

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Cococin® - Nourishing Factor

oconut water is often described as a «natural isotonic sports drink», providing higher amounts of electrolytes such as potassium and magnesium than conventional sports drinks. Coconut water solids also have a prebiotic role, and beneficially influence the balance of microflora in the gastrointestinal tract. When topically applied, coconut water solids nourish the skin and hair, supporting healthy cell growth. Cococin®, The Nourishment Factor® captures the goodness of coconut water in a convenient, free flowing, and powder form. Recently affirmed GRAS for use in foods and clinically validated for its topical benefits, Cococin finds multifunctional applications in cosmetic, personal care and food and beverage compositions. Produced under a patented lyophilization process, the amorphous nature of the solid produced by this process protects the protein components and environment sensitive actives during subsequent pulverization and storage. During storage, the material transforms to the more stable, less hygroscopic, crystalline state. Cococin blends seamlessly with cosmetic compositions, and easily disperses in water, making it a compact and versatile nutrient pool for use in topical formulations, as well as in functional foods & beverages that nurture from the inside out.

Nutritionally versatile

It is important to differentiate between coconut milk and coconut water. The ratio of RNA-phosphorus to DNA- phosphorus is

significantly lower in the liquid endosperm of mature coconuts as compared to that of green coconuts. RNA plays an important role in amino acid transport and respiratory metabolism in living cells. Coconut water used to make Cococin is obtained from green coconuts at the optimal stage of maturity, to ensure a high content of RNA and growth factors, including shikimic acid, quinic acid and indole-3-acetic acid, along with essential vitamins, amino acids, and minerals.At the completion of growth, the solid endosperm and the last of the coconut water provide nourishment for the forming embryo and seedling. Thus, coconut water serves the role as a reservoir of nutrients to support tissue growth (Tulecke, et. al; 1961). Coconut water has been used in the tropics as a nutritive and rehydrating agent to restore electrolyte balance in cases of diarrhea (Adams, et al.; 1992). A published research report mentions that coconut water can be used as a short term intravenous (IV) fluid (CampbellFalck et al.; 2000). Other reported applications include use in total parenteral nutrition (TPN) (Petroianu, et al.; 2004), and sports beverages (FAO, 1998) Cococin is The Nourishment Factor® that provides a valuable nutrient pool for enhancing food and beverage, as well as cosmetic product formulations (see in the osmetic part). Cococin can be safely used in oral care as well as lip care compositions. Cytokinins and several important minerals in Major (mm/l: Ca,K,Mg,Na, ), or Minor (μm/l: B,Mn,Zn…) or trace quantities (nm /l: Al, Cu,Mo…) are found in coconut water.

The natural patented proprietary formulation of 6 plant extracts (seeds and flowers), clinically proved for water elimination and detoxification. Water soluble, it’s the new solution for uses in beverages, sticks, as well as - or course - tablets. ElimReal® is an exclusive and patented combination of extracts from : • • • • • •

Carvi’s seeds (Carum carvi) Meadowsweet Flower buds (Spirea ulmaria) Guarana’s seeds (Paullinia cupana) Goldenrod’s plant (Solidago virgaurea) Fennel’s fruits (Foeniculum vulgare) Dandelion’s leaves (Taraxacum dens leonis)

Coconut water, the liquid endosperm of green coconuts (Cocos nucifera), is a refreshing natural beverage, an oral re-hydrating agent, and a supplement in nutrient media for tissue culture. Cococin is even more than that ... Coconut water, the liquid endosperm of green coconuts (Cocos nucifera), is a refreshing natural beverage, an oral rehydrating agent, and a supplement in nutrient media for tissue culture. Often described as a “natural isotonic sports drink”, providing higher amounts of electrolytes such as potassium and magnesium than conventional sports drinks, it also have a prebiotic role, and beneficially influence the balance of microflora in the gastrointestinal tract. It’s so ideally finds important applications in: à Rejuvenative health drinks à Sports drinks and à Kids nutritional support formulations On personal care and cosmetic formulations, it supports cell growth of human tissues such as hair follicles. It is non-irritating to the skin and is rich in nutrients that provide moisturization and support blood circulation. With continued use, this product can also improve skin elasticity and skin tone and decrease skin roughness, while nurturing keratinous tissue to potentially reduce the appearance of the signs of aging.

Elimination & Detoxification ElimReal is covered by a patent for slimming applications, and promotes renal elimination functions (excretory function of the kidneys) / urinary elimination /urinary volumes, so works on detoxification and slimming. The double-blind clinical study against placebo on 95 women for 1 month demonstrates its effectiveness : à Increase urinary frequency : (the subjects go to the toilet more often, a perfect marker of efficacy for subjects) 76,92% of the women who took ELIMREAL® under its tablet form observed an increase of their daily urination after 28 days. à Increase of the volume of urinary excretion of the day, thus participating in decongesting and detoxifying the body 58,34% of the women who took ELIMREAL® under its tablet form observed an increase of their 24h urinary volume after 14 days

à Increase sodium excretion, analytical objective marker of efficiency Significant Increase of Sodium excretion for both groups which receive Elimreal ® ( 10,69 % and 8,78 % in the groups 1 and 2 respectively) in opposition to a decrease of -15,97% in the placebo group based on plants extracts with an authorization of the components inmost members of the European Union. Elimreal is supported by very strong allegations , especially linked to the contribution of fennel extract. Recommended dosage / proofs of efficacy: a randomized clinical double blind versus placebo study demonstrated the efficacy of Elimreal at the dose of 805 mg/day) BECARRE Natural - October 2020 - 33

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actoSpore® is a lactic acid producing Bacillus preparation providing 15 billions of spores per gram.

After ingestion (medium for awakening), the spores revive and produce acid L (+) Lactic, with the positive action on other micro-organisms. Lacstospore is used in dietary supplements, in dairy products, bakery products, in tablets or vaginal ovules and in veterinary products.

Bacillus coagulans as a probiotic Clinical studies have revealed that L. sporogenes can be successfully implanted in the intestine : it satisfies the essential requirements of an efficient probiotic. Preparations of L. sporogenes in pharmaceutical dosage forms such as tablets, capsules, dried granules or powder have the following characteristics: Contain a large number of viable lactobacilli that retain viability during preparation in pharmaceutical dosage forms and during storage before consumption. The spores are thermostable as against viable L. acidophilus cells which may not withstand spray drying. Survive in gastric secretions and bile of the upper digestive tract and reach the intestine safely.

FOCUS HINTS

Settle in the digestive tract and produce enough lactic acid and other antagonistic substances to inhibit the growth of pathogenic bacteria. Being sporulated, they germinate under favorable conditions and produce sufficient viable cells which proliferate and perform vital healthful functions as described earlier. In addition, L. sporogenes spores are semiresident and are slowly excreted out of the body (7 days after discontinuation of administration).

Clinical Studies Different studies are available, and detailed on the website. We just mention few of them. Gastro-intestinal and associated effects : administration of L. sporogenes markedly improved the general clinical condition of the subjects and provided relief from intestinal disorders and allergic skin conditions. Studies performed on patients suffering of gastrointestinal disorders proved the efficacy on : à Acute and chronic intestinal catarrh à Diarrhea à Constipation à Abnormal intestinal fermentation à Dyspepsia infantum à Allergic skin diseases à Etc... Hypocholesterolemic effects : Total serum cholesterol, LDL-cholesterol and total cholesterol to HDL-cholesterol and LDL-cholesterol to HDL-cholesterol ratios (p < 0.001) was reduced significantly over a period of three months. Non-specific vaginitis : increase the vaginal acidity by the action of the lactic acid (pro34 - BECARRE Natural - October 2020

Probiotic Lactobacillus Lactospore® duced by Lactobacillus) Veterinary probiotic (weight gain, feces)

Recommended dosage Stability The daily recommended dose is 100 to 200 Million organisms per dose - 3 times daily, in tablets, capsules, chewable tablets, vaginal tablets or ovules, etc... Recommended dosage in feed and veterinary products are upon request. Sabinsa has studied the heat stability at 90°C and 140°C (stability on viable count of Lactospore with high temperature) and the viable counts remain over 90% even after 150 sec at 90% or 30 sec at 140°C. Virtually 100% of spores are revived, one reason for the choice of Bacillus coagulans (sporogenes), and specific drying method. Spores will not grow during their action, and disappear by natural ways after working, hence the need for regular intake.

New Safety, Stability And Efficacy Studies Published LactoSpore: Anti-diarrheal & Gastrointestinal motility study (International Journal of Pharma and Biosciences. 7 (1): 311-16. 2016) The effect of Bacillus coagulans MTCC 5856 (LactoSpore) on castor oil induced diarrhoea and gastrointestinal (GI) motility using well established in vivo studies found LactoSpore exerted dose dependent anti- diarrhea activity and reduced the faecal output (33%). It also inhibited the gastrointestinal motility. The results suggested that LactoSpore possess significant anti-diarrheal activity and therefore could be a potential agent in the management of diarrhea. LactoSpore in treatment of diarrhea predominant IBS study (Nutrition Journal.15:21. 2016) A double blind placebo controlled multicentered trial was conducted to evaluate the safety and efficacy of LactoSpore in diarrhea predominant Irritable Bowl Syndrome (IBS) patients. Thirty-six newly diagnosed diarrhea predominant IBS patients were enrolled in three clinical centers. Along with standard care of treatment, 18 patients in group one received placebo while in group two, 18 patients received LactoSpore for 90 days. The study concluded that LactoSpore along with standard care of treatment was found to be safe and effective in diarrhea predominant IBS patients for 90 days of supplementation. Hence, LactoSpore could be a potential agent in the management of diarrhea predominant IBS patients. LactoSpore: Genetic and Phenotypic Consistency Findings (World Journal of Microbiology and Biotechnology 32:60. 2016) That B. coagulans strain is stable over multiple years of commercial production was clearly

Shelf-Stable Probiotic Lactospore® Easy to use probiotic providing 15 billion Lactobacillus sporogenes spores (also called coagulans), stable. Activities are multiple, interacting for the whole body, including digestion, cholesterol management and health of the intestinal tract.

demonstrated through this study designed to evaluate probiotic potential, in-vitro safety, product stability, genetic and phenotypic consistency of the probiotic strain used for production of LactoSpore. Phenotypic and genotypic studies proved that the commercial preparations of LactoSpore contained the same strain of B. coagulans MTCC 5856 over multiple years of commercial production. B. coagulans MTCC 5856 strain showed probiotic potential and found to be non-mutagenic, non-cytotoxic, negative for enterotoxin genes and stable at ambient temperature (25 ± 2°C) for 36 months. Findings of this study provide confidence to use B. coagulans MTCC 5856 as a probiotic ingredient in various dietary and functional foods and health products. LactoSpore Safety Study (Journal of Clinical Toxicology 6: 283. 2016) A Double-Blind, Placebo-Controlled, Parallel clinical trial was conducted to evaluate the Safety and tolerability of B. coagulans MTCC 5856 at a dose of 2 billion spores per day in healthy adults. Safety and tolerability of B. coagulans MTCC 5856 was assessed over 30 days by safety laboratory parameters (blood hematology and clinical chemistry parameters), anthropometric measures (weight, BMI, blood pressure and heart rate), adverse events, Bristol stool score, tolerability questionnaire and bowel habit diary. LactoSpore has recently obtained the approval as Natural Health Product (NHP) that allows formulators and marketing companies to market LactoSpore in Canada as NHP. This approval renders Canadian market readily accessible by US and Canadian marketing companies. Pre-approved NHP ingredients can reduce the review period of Product license application in Canada, where a pre-market approval system precedes actual marketing. LactoSpore’s approval in natural health products in Canada is also supported by Sabinsa’s Utah facility, a registered foreign site for manufacturing the NHP for the Canadian market.

Ultimate Nutrients Enhancer BioPerine® is the product sourced out of piperine studied for its ability to increase the bioavailability of nutritional compounds. It is also the only source from piperine to have undergone clinical studies to substantiate its safety and efficacy for nutritional use.

BioPerine® :

à is Nature’s Bioavailability Enhancing If you lack the one ingredient that can change the way your body looks, just go for BioPerine®

broad range of nutrients that contribute to metabolism, i.e. vitamins, minerals, herbals, amino acids, etc. It is as if BioPerine® activates a metabolic paddle wheel, of sorts, that selectively provides a more efficient mode of nutrient transportation into the blood. Non specific possible mechanisms promoting rapid absorption of nutrients : à Increases blood supply to the GI tract à Increases emulsifying content of the gut à Increases active nutrient transport

à has been studied by Sabinsa and many external labs

Advantages of BioPerine®

à is a standardized extract from the fruit of Piper nigrum L (black pepper) à contains 95% of piperine à may be co-administered with various nutrients for both human and animal health.

à is provided with full safety data Get more for your body from this supplement.

How does BioPerine® work?

Bioavailable curcuminoids Effect of BioPerine® on Serum Concentrations of Curcumin in Human Volunteers. The relative bioavailability is improved by more than 20 times when therapeutic effectiveness of curcumin is often limited due to its poor absorption from the GI tract.

Bioavailable b-Caroten BioPerine® increased the absorption of co-administered beta-carotene in human volunteers by 160%, using 5mg BioPerine®.

Bioavailable Selenium Effect of BioPerine® on serum selenium levels during a 6 week supplementation trial in human volunteers : increase by 145% of the selenium levels over the presupplementation value (50 µg of elemental selenium in the form of Selenomethionine versus 50µg+5mg BioPerine®).

The metabolic process that generates energy at the cellular level in the human body is called thermogenesis. Though thermogenesis has been identified as a key factor in maintaining weight loss, it has also been identified as playing an integral role in utilizing the daily food and nutrients that the human body consumes. It sets in motion the mechanisms that lead to digestion and subsequent gastrointestinal absorption. Piperine, in the patented form of BioPerine® enhances the body’s natural thermogenic activity-hence the term Thermonutrient®. This enhancement may be explained as a means of increasing the thermal energy sufficient to «power up» the mechanism related to thermogenesis. This in turn results in increased metabolic processes that creates a «demand» for «supply» of a

Dosage

Bioavailable vitamins Efficacy of BioPerine® (5 mg) on the bioavailability of Vitamin B6 absorption in human volunteers : increase by 250% of the vitamin B6 serum level after 2 hours compare to control (100mg B6 / 100mg B6+BioPerine®).

Bioavailable Resveratrol Many other studies have been conducted, the most recent one on the resveratrol : “We found that the degree of exposure (i.e. AUC) to resveratrol was enhanced to 229% and the maximum serum concentration (C max ) was increased to 1544% with the addition of piperine” (Mol. Nutr. Food Res. 2011, 55, 1–8).BioPerine® has legitimate application in the field of nutrition, particularly since we are becoming increasingly aware of the impact of nutritional deficiencies on our health.

Bioavailable CoQ10 Effect of BioPerine® on serum CoQ10 levels during a 21 day supplementation trial (+150% in serum, 90mg CoQ10+5mg BioPerine®).

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The recommended dosage is 5mg per serving dose. Acute, subacute and chronic toxicity studies of piperine in laboratory animals indicate that piperine used even in a broad range of doses does not cause abnormalities in the general growth pattern, body to organ weight ratio, clinical symptomatology, or blood chemistry. The dose of piperine considered to be bio enhancing for absorption of nutrients is calculated as 0.04 to 0.08 mg piperine/kg body weight. That dose is 4,000 times less than the LD50 dose (dose toxic to 50% animals tested) of piperine established in mice and rats. Incidentally, the dose of piperine, which increased the bioavailability of the actives studied, was several times lower than the estimated amount of piperine consumed daily in the diet by an average individual in the USA (Majeed, M. et al.; 1999).

BioPerine® is the only product sourced out of piperine to obtain a patented status for its ability to increase the bioavailability of nutritional compounds. Secondly, it is the only source from piperine to have undergone clinical studies in the U.S. and in other contries to substantiate its safety and efficacy for nutritional use. It’s an objectivated product, with a lot of sciences behind, for your kind support.

Bioavailability Enhancer Bioavailability is “the quantity of a substance, either nutrient, drug, or toxicant, that effectively reaches the target cells where it modifies, for good or bad, their metabolism and consequently their fate” (Basu, T.K). The nutritional materials which may be co-administered with BioPerine® are the following groups: à Herbal extracts : (e.g.Curcumo, Boswellic acids, Ashwagandha, Ginkgo biloba and Capsaicin) à Water-soluble vitamins : (e.g. Vitamin B1, Vitamin B2, Niacinamide, Vitamin B6, Vitamin B12, Folic acid and Vitamin C) à Fat-soluble vitamins : (e.g. Vitamin A, Vitamin D, Vitamin E, and Vitamin K) à Antioxidants : (e.g. Vitamin A, Vitamin C, Vitamin E, alpha-carotene, beta-carotene, beta-cryptoxanthin, lycopene, lutein/zeaxanthin, pine bark bioflavonoids complex, germanium, selenium and zinc). à Amino acids : (e.g. lysine, isoleucine, leucine, threonine, valine, tryptophan, phenylalanine, and methionine) à Minerals : (e.g calcium, iron, zinc, vanadium, selenium, chromium, iodine, potassium, manganese, copper and magnesium). If one believes that since BioPerine® is sourced out of black pepper, all one need to do is increase the consumption of black pepper. Again you have gone wrong, for the direct intake of black pepper will not help achieve enhanced nutrient absorption.

BECARRE Natural - October 2020 - 35

FOCUS

haponticum carthamoides, also known as Leuzea, or Maral root, is plant indigenous to the Lake Baikal region and distributed throughout Eastern Siberia. Traditionally the Siberians consumed the Leuzea tea mixed with Rhodiola rosea, in cases of tiredness and general weakness after illness and energizing remedy after the long Siberian winter.

Traditional Often used to improve athletic performance and build muscle mass, and improve libido, maral root is mentioned to treat a variety of health issues. The root contains compounds that can influence health, including antioxidants and plant-based steroids called ecdysteroids.

Powerful natural anabolic steroids Maral root has adaptogenic properties similar to ginseng root (Panax ginseng), replenishing energy stores, increasing libido, sharpening focus, promoting lean muscle growth, reducing body fat, improving mood and stimulating the immune system.

FOCUS HINTS

Key active constituents is a mixture of compounds called, “levseins”n representing a complex of more than 10 ecdysterones. 20HE (20 ecdysteroids) have been reported to have anabolic effects, and different preparations tested in human trials have shown diminished fat content with elevated muscle mass, increased working capacity in top athletes.

Ecdysterones from Rhaponticum Rhaponticum carthamoides standardized to min. 1% natural ecdysteroids (or ecdysones) of plant origin

Free from the side-effects of anabolic steroids

In a placebo-controlled experiment conducted on 112 athletes, 89% of those taking Rhaponticum found their fatigue passed more rapidly, were less lethargic following physical exertion and performed better in sports such as athletics, swimming, speed skating and alpine skiing without negatively affecting the adrenal cortex. Neither did it have the androgenic effect of anabolic steroids, nor their associated side-effects.

Performances During intense physical activity, Rhaponticum extract inhibits energy metabolism disorders, maintains stable levels of glycogen in skeletal muscles, increases blood supply to muscles and brain, and shortens the recovery period after prolonged muscle workload. Studies show that the work capacity of the athletes increased by 10-15%, as well as muscle muscle cell growth.

Anti-fatigue A study versus placebo conducted on 50 sailors shows for the Rhap. group: sleep normalised, appetite increased, mood was better and, above all, their productivity increased.

Libido Rhap. extract also stimulates sexual function, increased copulative function and improved sperm quality (Mirzaev IR et al).

Digezyme

Research conducted in Russia suggests that extract of Rhaponticum carthamoides may significantly increase muscle mass while decreasing fat mass in athletes when taken during training. Studies also show it may help boost capacity for work and endurance, and reduce physical and mental fatigue.

Included in the Soviet pharmacopoeia in 1961 as a remedy for increasing capacity for work, athletic performance and recovery following strenuous muscle work

It helps free nutrients in both fruits and vegetables à Lipase: Breaks down lipids and improves fat utilization and also supports healthy gallbladder function

The process of enzymatic digestion of food in the body may be impaired by several factors : Stress due to sudden changes in food consumption patterns, exposure to environmental pollutants, vagaries of the weather, extensive travel. Somatic diseases that necessitate treatment with surgical procedures, antibiotics and immuno-suppressive drugs.

This group of enzymes breaks carbohydrates, proteins and fats and all three groups of enzymes are resistant to the action of gastric juices, while retaining their digestive activity. DigeZyme is referenced in The Complete Book of Enzyme Therapy by Anthony J. Chichoke (Ref. 1). DigeZyme was also clinically evaluated for enhanced absorption of minerals and vitamins.

The sites of enzyme-production, such as the pancreas could also be damaged, by disease or injury, leading to enzyme insufficiency.

Why Digezyme ?

Some individuals may suffer from an inherent lack of specific enzyme generation systems, due to genetic factors, resulting in digestive and metabolic disorders

Five enzymes DigeZyme consists of five GMO-free broad acting enzymes obtained from the fermentation process: à Amylase : breaks down carbohydrates such as starch and glycogen, into smaller units à Protease: Breaks down protein and supports immune function à Lactase: Breaks down lactose (milk sugar) and useful for lactose intolerance à Cellulase: Breaks down cellulose and chitin. 36 - BECARRE Natural - October 2020

This multi enzyme complex help in the digestion of components when poorly assimilated by our bodies in cases of unbalanced diets, fatigue, or impaired pancreatic function - or in case of intolerance. Digezyme helps to improve overall health and nutritional status : à Reduce toxic load generated by undigested food materials à Cleanse the colon à Strengthen the immune system à Facilitate restful sleep à Benefits for Delayed Onset Muscle Soreness And other activities related to a good digestion : à Enhance mental capacity à Help shed excess weight and fat à Prevent aging of the skin

Digezyme is an easyready-to-use multi enzyme complex to help in the digestion of components, and against muscle soreness Digezyme is so not only used to for those who experience a chronic gastrointestinal discomfort (e.g. gas, constipation, diarrhea, cramps) or preventive in the middle age and elderly individuals.

Dose & Quality The daily dose is 50mg per dose, three times daily, in tablets, capsules or even powdered diet mixes. Digezyme is easy to use: the activity starts when the enzymes meet the specific conditions (humidity, pH, substrate…) they need, with enough reaction time in presence. Digezyme is a formulation of food enzymes (nonanimal origin). It contains Lactase, what got an approval by Efsa (extra lactase may be needed for the claim, depending on the dosage of Digezyme).

FOCUS

Slimming with Forslean Forslean® is manufactured by a proprietary process and is a standardized extract from the roots of the Coleus forskohlii plant, the only known plant source of forskohlin. ForsLean® is a registered trademark of Sabinsa Corporation and is the only Coleus forskohlii preparation supported commonly by Patent.

orslean® is Sabinsa’s new proprietary composition extract of Coleus forskohlii root, standardized for Forskolin. Forslean® has shown promising results in three areas; enhancing lean body mass, promoting fat loss and promoting weight loss. In September of 1998 Sabinsa was granted a use patent for this application of Forskolin in its Forslean® composition.

duction of cyclic adenosine monophosphate (cAMP), a significant biochemical agent in metabolic processes. The role of cyclic AMP is indispensable to many body functions. It induces a chain reaction of biochemical events that trigger metabolic processes and diet induced thermogenesis, thereby providing the means to maintain healthy body composition and lean body mass levels.

Intended Application

Double blind studies

The importance of maintaining or regaining lean body mass has recently come to light for two important reasons. First is the increased recognition that lean body mass plays a vital role in any successful weight training regimen, and second, there is a growing awareness that lean body mass is proportionate to the overall health of an individual.

Dozen studies are available. In one on 12 weeks, 25-45 y, 60 obese males and female volunteers, Forslean® treated volunteers shed on an average 1.73 kg from their body weight, in comparison placebo group gained 0.25 kg.

Lean body mass is composed of muscle, vital organs, bone and bone marrow, connective tissue and body water. The percentage of lean body mass to fat not only determines the body’s aesthetic appearance, but more importantly, it is also an index of physical fitness, health status, susceptibility to disease and premature mortality. Because the body’s metabolic rate is directly proportional to the amount of lean body mass, there is substantial interest in products that safely increase lean body mass because they are most likely to work. The use of Forslean® may help to increase lean body mass and optimize body composition with one of the side effects being fat loss and/or weight loss. A sluggish metabolic rate is an undesired effect of many weight-loss regimens. It was observed in one study that formerly obese subjects had a 3-5% lower resting metabolic rate than control subjects. The occurrence of a low resting metabolic rate is likely to contribute to the high rate of weight regain in formerly obese persons. Clearly, we need to change and broaden our thinking on the objectives of weight management regimens for both active and notso-active individuals. In particular, it should be emphasized that healthy functioning of the body depends not so much on a lower fat content, but rather on obtaining a higher percentage of lean body mass.

Sustainability Sabinsa is growing Coleus in part of its 44 000 acres, and do the extraction in its own plant selection fully dedicated to the production of Coleus extracts, to provide you the best quality and the best sustainability. Last Safety studies Acute, Sub-Acute, Chronic Oral Toxicity and Mutagenicity of Coleus forskohlii Briq. Hydroethanolic Extract was published in the International Journal Of Pharmacy & Pharmaceutical Research December 2015 Vol.:5, Issue:1

Again, it should be kept in mind that it is not only fat, but also lean body mass that is, or can be, lost through dieting. This fact often escapes our attention when we reduce our total body weight. The loss of lean body mass offsets any benefits derived from the reduction of body weight, and can potentially increase one’s chances for diabetes, cardiovascular disease and possibly some forms of cancer due to poor metabolic activity.

During the 12 – week period of treatment volunteers treated with placebo gained 0.68% of body fat. On the other hand, the Forslean® treated group lost 0.46% of body fat. In the group of volunteers that received Forslean®, there was an increase in the LBM as compared to the placebo group where, there was a decrease in LBM. HDL cholesterol showed a statistically significant increase in volunteers treated with Forslean®. Other serum lipid profiles remained statistically unaltered in the Forslean® and Placebo treated groups. 50 subjects, male and female, were randomized to receive 250 mg of ForsLean® capsules twice a day for 12 weeks. A significant decrease in body weight and fat content and a significant increase in lean body mass were observed (Kamath, M.S. et al (2004)). The mean percentage lean body mass increased by 1.78% in the ForsLean® group, while the placebo group showed a mean decrease of 0.2% of LBM from baseline values. The mean percentage body fat content in the ForsLean® group was 34% as compared to 39% in placebo receiving group at the end of the 12-week trial

Dosage Form Forslean® has been clinically evaluated at one dose, 250 mgs twice daily. This provides 50 mgs of forskohlin, the primary active compound in Forslean®. Dosage has been in the form of a two piece, hard shell capsule.

Toxicity Acute toxicity LD50 : 2g / kg Chronic toxicity : none at 1g / kg on 6 months Mutagenicity : non-mutagenic (AMES). Salmonella t., and E. coli also controlled. Safety : A number of clinical studies investigating the efficacy of Forslean® for body composition management have been conducted, and parameters related to safety have been monitored. No significant changes in average systolic and diastolic pressures (12 weeks, 60 volunteers). None even on thyroids hormones. There is a significant positive change (p<0.05) in the concentration of HDL. BECARRE Natural - October 2020 - 37

NEWS

New scientific article on Cognitive Function for Zynamite®

Acute Effects of a Polyphenol-Rich Leaf Extract of Mangifera indica L. (Zynamite) on Cognitive Function in Healthy Adults: A Double-Blind, PlaceboControlled Crossover Study” The article is based on a recent CNS clinical study in collaboration with Prof Kennedy from the Northumbria University in the UK: 300mg Zynamite® , single dose, N=70, cross over design, healthy subjects. Briefly : - Zynamite® improves concentration, attention & memory - Onset already 30min after intake, lasts longer than 5 hours

Sabinsa’s pTeroWhite® Subject of Two New Published Studies Reporting Anti-Aging, Skin Brightening, and SPF Benefits.

Two new studies have recently been published on the topical benefits of Sabinsa’s pTeroWhite® natural pterostilbene isolated and purified from Pterocarpus marsupium heartwood and formulated as a cream. The studies further the body of science into pTeroWhite®’s value as a cosmeceutical ingredient for anti-aging, skin brightening, and as a natural SPF agent Name: pTeroWhite® INCI Name: Pterocarpus Marsupium Wood Extract CAS No Pterostilbene: 537-42-8 (trans)

PREVIOUS NEWS

pTerosol™ and blood sugar management

pTerosol™ by Sabinsa is the Pterocarpus marsupium extract, standardized to 5% of C-glycosides, offers you a new alternative for Blood Sugar and Cholesterol Management. Tests have been already conducted and results of pre-clinical study show: • Serum glucose level : significant reduction, dose-dependant • Plasma Insulin : significant increase, dose-dependant • Total cholesterol, TG, and LDL: significantly decreased • HDL level : significantly increased • Glycosylated hemoglobin (HbA1c) : significantly reduced, close to the normal value • Significant reduction of TNF-α and IL-6

ESSNA awards

The European Specialist Sports Nutrition Association [ESSNA] honored Nektium in a total of three categories: • Zynamite®: Best “free from” product • Zynamite®: Highly commended “2019 Game changer” • Rhodiolife®: Best Targeted Sports Nutrition Product 50+

TinoFolin® / Memory

TinoFolin - new name of the extract of Tinospora cordifolia - was evaluated for its ability to improve visual memory, logical memory, verbal memory, attention span and concentration in a placebocontrolled double-blind study involving 30 human volunteers 38 - BECARRE Natural - October 2020

A selection of our previous news and innovations Lactospore® / Depression

Sabinsa’s Shelf Stable Probiotic LactoSpore® Found to Reverse Depression Symptoms in Patients Suffering From IBS According to Published Study.

DigeZyme® / Dyspepsia

Sabinsa’s Multi-Enzyme Complex DigeZyme® Demonstrated Efficacy in Management of Dyspeptic Symptoms with Functional Dyspepsia: Published Clinical Study.

Alertness and focus

Papain and Bromelain enter our catalogue

Processed in India by a dedicated company specialized on enzymes, taking care of sourcing and quality. Those two enzymes complete the range with the well known Digezyme.

Lactospore® / Cholesterol

Sabinsa’s Shelf Stable Probiotic LactoSpore® “May Improve Cholesterol Levels”, Study Suggests.

Promond™ - Almond protein powder for sport

Promond™ almond protein is a water-soluble 50% protein sourced from Indian variety of almonds. It is water extract with a complete array of amino acids, including high levels of BCAAs. Although its amino acid profile is similar to whey and pea protein, is particularly high in glutamate, arginine, aspartate, and phenylalanine. Those concerned about almonds grown in California because of heavy water use : Promond™ is made from almonds sourced in northern India, out of over-irrigation.

DigeZyme® and Muscle Soreness

Sabinsa’s DigeZyme® shows benefit for delayed onset muscle soreness in published study. It was shown to have the potential for more rapid recovery from Delayed Onset Muscle Soreness (DOMS) in a study published in Sports Nutrition and Therapy: Multi−Enzyme Complex for the Management of Delayed Onset Muscle Soreness after Eccentric Exercise: A Randomized, Double Blind, Placebo Controlled Study. DigeZyme® is the proprietary well-known blend

of non-animal enzymes. Beside the use to assist digestion, it was shown to have the potential for more rapid recovery from Delayed Onset Muscle Soreness (DOMS) in a study published in Sports Nutrition and Therapy (20 healthy male subjects, DOMS compared to placebo).

Boswellin® osteoarthritis of the knee

Boswellin Super, our Boswellia serrata extract at 30% AKBBA, was found to alleviate knee pain in healthy adults in a new study. Results of the randomized, double-blind, placebocontrolled trial, published in The European Journal of Biomedical and Pharmaceutical Sciences, also suggest the boswellia extract inhibited secretion of hyaluronic acid into blood. In a double blind vs placebo controlled trial, results with Boswellin® shows great efficacy in the management of osteoarthritis of the knee. X-Ray of knee shows it increase the space between the knee joints. The pain also decreased, more and longer than with Valdecoxib. Different other parameters have been controlled (WOMAC, Hs-CRP, etc...)..

Rhodiolife® and the immune system

The Rhodiola extract Rhodiolife® has clearly demonstrated its ability to protect cells from viral infection. This is the first time shown to be effective in the activation of the human immune system - Ahmed M, et al (2015) «Rhodiola rosea Exerts Antiviral Activity in Athletes Following a Competitive Marathon Race». Front Nutr. Jul 31;2:24.).

Rhodiolife® improves memory and increases brain activity

Rhodiola rosea is known to improve attention and cerebral performances. On 2016, Polinat has conducted new studies: Electroencephalic studies (ECG) showed that animal supplemented with Rhodiolife® have an increased neurotransmitter production in the studied areas. This effect lasts at least for 5 hours until the end of measurements, and so even more. Besides of a stimulatory effect, the results indicate an improve of memory and an increased activity in brain regions related to motivation and reward.

Preserved actives from Pomegranate P40p™ is the unique carrier-free Pomegranate extract standardized to the whole spectrum of the real actives offered in pomegranate.

P40P™ brings the punicosides and more specifically the highly potent punicalagins (ellagic acids are only the metabolized compounds of those ellagitannins across the mitochondrial membrane), the highly active carbonic anhydrase inhibitor and the major player behind the antioxidant capacity of pomegranate juice. P40P™ is soluble in water and has high bioavailability.

NEWS

A selection of our previous news and innovations Bio-efficace SOD GliSODin® and oncology

Different studies have shown the great interest of our clinically proven enhanced SOD and oncology / radiations. It includes recent published ! “Impact of Superoxide Dismutase-Gliadin on Radiation-induced Fibrosis” and “stress to γ-radiation”. GliSODin® has been also evaluated for its prevention of inflammation of benign fibrosarcoma cells, and effective role in azoxymethane-induced colon cancer in animal model suggesting it can act as an effective chemo-preventive agent.

GliSODin® in accelerating symptom relief in children with allergy

In the present randomized, placebo - controlled, double - blind clinical trial, the clinical efficacy of GliSODin® in accelerating symptom relief in children with allergic asthma and house dust mite allergy (receiving house dust mite immunotherapy) was demonstrated. Forty subjects aged 6–17 years old with asthma, tested positive for house dust mite allergy on skin prick test, and received immunotherapy were enrolled in this study; “Use of Superoxide Dismutase in Accelerating Symptom Relief in Asthmatic and House Dust Mite Allergic Children Receiving House Dust Mite Immunotherapy”, Anang Endaryanto, Zahrah Hikmah, Ariyanto Harsono

Curcumin C3 Complex and omega-3

An excellent complementary supplement : while

curcumin is a well-recognized anti-inflammatory agent, Omega-3 acids are known precursors of several biosynthetic inflammatory resolvers. It was found that C3 Complex attenuates muscle wasting (cachexia) that usually accompanies certain disease conditions [British J Nutrition (2009)]. Recent mechanistic studies revealed that the synergistic effect of C3 Complex® and Eicosapentaenoic acid, an Omega3 component, against the depredations caused by both TNF-a and PIF (Proteolysisinducing Factor) by ameliorating protein synthesis and halting protein degradation. [In Vitro Cell. Dev. Biol.] Experiments presented in Experimental Biology Meeting 2016, San Diego showed a clear synergy in action in reducing cancer cachexia in MAC-16 colon tumor-bearing mice. This combination Omega-3 EPA and C3 Complex is expected to attenuate weight loss caused by health conditions, and improve quality of life. [Abstract, the FASEB Journal]

Curcumin C3 Complex® - Body fat and interleukin-1β

In an animal study on HFD (high fat diet)-fed mice extending 53 weeks, the scientists concluded hat the nutrient combination, curcumin-piperine, works well to reduce total body fat, reduces cellular aging as indicated by preserving telomere length, and enhance the CR effects for the prevention of metabolic syndrome by reducing the low grade chronic inflammation that always persists in obese conditions

Curcumin C3 Complex and metabolic syndrom

A significant study on Curcumin C3 Complex® (in combination with BioPerine®) in patients with metabolic syndrome has been published on end of 2014, showing the significantly greater effect of Curcuminoids in reducing the serum concentrations of LDL-C, non-HDL-C, total cholesterol, triglycerides and Lp(a), when serum HDL-C concentration was elevated significantly. Serum sdLDL levels were comparable (randomized, doubleblind, placebo, 117 patients, 8 weeks).

Best Natural Extract Product Provider

GHP (Global Health and Pharma) has announced the winners of the 2017 International Life Sciences Awards. The International Life Sciences Awards focuses on a myriad of disciplines that fall under the ‘life sciences’ umbrella. Becarre Natural is proud to have been recognized for its work and continuous involvement in quality and information

ORAC > 1 200 000

FruitOx™ is the ingredient developped by Nektium with the idea of a fruits-based, water soluble and antioxidant natural extract, offering a wide and effective spectrum of anti-radical properties and stimulation of cellular defenses. Besides other proved properties, FruitOx™ has been controlled at its ORAC value of 1 259 340 (Trolox equivalent / 100g).

THE WHOLE PICTURE & THE WHOLE TRUTH

Cur cumin C3 Complex®

Launched in 1992; International patents in the following years. Sold in Europe before 97’ First standardized curcuminoids ingredient to receive a “no questions” letter by US FDA Most clinically studied curcumin brand History of use with proofs of efficacy & safety: more than 125 Bll doses sold w.w., alone or with BioPerine® BioPerine®

CLINICALLY STUDIED CURCUMIN BRAND

70+

PUBLISHED CLINICAL STUDIES

150+

RESEARCH PAPERS BECARRE Natural - October 2020 - 39 PUBLISHED

FOCUS

he plant decree was published on July 17, 2014 in the official journal, laying down the list of plants, other than mushrooms, authorized in food supplements and their conditions of use. Its implementation is January 2015, 1st. The list of fungi, and probably essential oils, will be the subject of further publication.

and quality) for extraction, purification, and any other step (elimination of compounds or hazardous compounds).

This decree includes 14 articles, describing the terms used and the applications, and 3 annexes.

Name , PER (Plant Extract Ratio: final ratio after extraction and before any other addition and the initial amount of extracted plant - dry /dry if it is a dry extract), DER (Drug Extract Ratio: ratio between the final ratio of the preparation and the initial amount, so after addition any additives or carrier ...). A product without carrier shows a PER = DER. Sometimes indicated, the NER is the native ratio

This text is intended for all players acting in the production, processing and distribution of food supplements. It refers to the other decrees of this sector, such as the European Directive 2002/46 / EC and its amendments, including national decrees (2006/352, 1170/2009). It gives the list of plants authorized on the market and / or mutual recognition by Article 16.

Other information related to the process, definition of a batch, size

Plant preparation (ex. Plant extract for BECARRE Natural)

Full composition (in %, with the nature and purpose of each component, purity criteria additives - 231/2012)

Annex I lists the 540 authorized plants (at the date of June 2014), with :

Standardization (markers, actives) with a detailed method of analysis (validated / internal validation) and the standard used as a reference

Their scientific name and family : synonyms exist in several cases . If necessary, you can also visit the website www.theplantlist.org

Substances to be monitored : with reference to Annex I or any other molecule needing to be monitored

Their vernacular name

Impurities (eg 2009/32), contaminants, bacteriological controls, other controls, see ... 1881/2006, 629/2008, 1259/2011, etc

Annex I

The part(s) used The substances to be monitored : it is not necessarily substances to be absent or undetectable, but substances that the decree specifically request to be checked. The restrictions of use: mainly specific labeling warnings (child, pregnant women), sometimes about the process.

Annex II

FOCUS HINTS

Our Reading of the Plant Decree

Annex II contains information that may be provided by operators in the sector in order to understand the preparation. As such, a model was proposed by Synadiet and Food Supplements Europe. This model is split into several parts. There is no obligation to complete each field, as indeed it’s just a modelform :

Plant (ie. Raw Material) Plant Description : Scientific name, vernacular name, family, existing monograph, risk of falsification (by another similar plant for example) Culture method (geographical origin, method of cultivation, specific agreements, licensing, 338/97) and collection (collected plant parts , mechanical or manual cleaning, drying) Parts of the plant used and its identification (controls, markers, purity) Production process (starting from the plant) Geographical location of the process (unit), manufacturer, preparation before process (grinding, separation, ...) Description of the extraction process from the raw material (ex. if it is extracted from an oleoresin, it is necessary to mention the process from the plant): solvent (concentration 40 - BECARRE Natural - October 2020

Statements, other data : nutritional data, GMO (1829 and 1830/2003) Irradiation (1999/2, 1883/2003), allergens (2007/68, 2000/13), ...) ... Stability data with the reference (ICH ?), does it need homogenization, ... In addition, BECARRE Natural completed the form of a preparation to additional food safety information when available, although Annex III is not needed.. à Exposure Level : consumption of the plant, of the extract à Risk analysis : toxicity studies, genotoxicity, mutagenicity, safety, NOAEL à Traditional use à Possible evolution of the process

Annex III This third part relates to the safety of the plant preparation, and it must be completed when there is a doubt, or when the plant preparation does not have enough history to justify its safety. Regarding the safety for the consumer, it is provided by the manufacturer of the food supplements, with the support in our view - of each manufacturer involved. Annex III may correspond to a ‘simple’ analysis of the literature of available toxicological data and / or made by the producer, as an extensive risk analysis in the case of conflicting studies, or molecules interaction, or whenever the specific analysis needs.

Which products are concerned by Annex III ? All products are concerned since the risk analysis must be done on all extracts but not all require a Annex III. It is important to understand that Annex III

The plants decree has been published July 17th, 2014 «laying down the list of plants, other than mushrooms, authorized in food supplements and their conditions of use». You’ll find here below a reading of the publication - especially annexes II et III is applicable when the plant preparation cannot refer to an existing and traditional use enough to guarantee the safety of a plant preparation (or food supplements), as it exists and has existed on the market. Thus, a plant extract may be traditionally produced in a PER of 15:1 and by using a high solvent (eg, pure ethanol). It is this extract which is known, and can prove its safety - and so would not need an Annex III. Speaking of food safety, we can refer only to what is clearly known.

Collect of information The traditional use can be justified by various ways, including probably monographs, references in pharmacopoeia (with specific description of the extract and not only of the plant), references to EMA, relation to the WEU (Well Established Use ), or from pharmaceutical agency, etc ... describing the extract (plant part, solvents and concentration processes, ratio), and with the Annex II to confirm that the plant extract that is offered is conform to traditional extract.

From Risk analysis to Annex III 3 possible cases

The preparation is described in the literature to demonstrate the history of use and safety. It is sufficient to indicate. The preparation is not described in the literature but the argument shows that the extract is equivalent to one described preparation. It should provide the rationale for comparing the described preparation and its extract. The preparation is not described in the literature and no equivalent that can be demonstrated: it is necessary to bring the safety analyzes - including toxicology - in relation to the identified hazards to be indicated in Annex III.

Italy : « Regulations on the use substances and plant preparations in food supplements »

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Notre Lecture de l’Arrêté Plante

’arrêté plantes a été publié le 17 juillet 2014 dans le journal officiel : « Arrêté du 24 juin 2014 établissant la liste des plantes, autres que les champignons, autorisées dans les compléments alimentaires et les conditions de leur emploi ». La liste des champignons, ainsi sans doute que les huiles essentielles, fera l’objet de dispositions ultérieures. Cet arrêté comprend 14 articles, décrivant les termes utilisés ainsi que les applications, et 3 annexes. Ce texte est destiné à tous les intervenants de la production, transformation et distribution de compléments alimentaires. Il est ainsi en référence aux autres textes de ce secteur, telle la Directive Européenne 2002 / 46 / CE et ses amendements, y compris nationaux (2006/352, 1170/2009). Il indique la liste des plantes autorisées en vue de la mise en marché et/ou reconnaissance mutuelle par article 16.

Annexe I L’Annexe I liste les 540 plantes autorisées (à la date de Juin 2014), avec : Leur nom scientifique et la famille : plusieurs cas de synonymes existent. Au besoin, vous pouvez également vous aider du site www. theplantlist.org Leur nom vernaculaire Les parties utilisées Les substances à surveiller : il ne s’agit pas nécessairement de substances qui doivent être absentes ou non détectables, mais des substances que l’arrêté demande de vérifier spécifiquement. Les restrictions d’utilisation : essentiellement des étiquetages spécifiques de mise en garde (enfant, femmes enceintes), parfois des process.

Annexe II L’Annexe II présente les informations susceptibles d’être communiquées par les opérateurs du secteur afin de bien comprendre la préparation. A ce titre, une modèle a été proposé par Synadiet et Food Supplements Europe. Ce modèle est décomposé en plusieurs parties. Il n’y a pas obligations de compléter chaque champ, s’agissant bien d’un modèle :

Plante (ie. Matière première) Description de la plante : Nom scientifique, vernaculaire, famille, monographie existante, risque de falsification (par une autre plante similaire par exemple) Méthode de culture (origine géographique, mode de culture, accords spécifiques, licenses, règlement 338/97) et de collecte (parties de la plante collectée, mécanique ou manuelle, n e t toya g e, séchage)

Partie de la plante utilisée et son identification (contrôles, marqueurs, pureté) Procédé de production (partant de la plante) Localisation géographique du process (unité), producteur,préparation avant process (broyage, séparation, …) Description du procédé d’extraction depuis la matière première (ex. s’il s’agit d’une extraction depuis une oléorésine, il convient de mentionner le process avant) : solvants (concentration, qualité) pour l’extraction, purification, ainsi que toute autre étape (élimination de composés ou de risques). Autres informations liées au process, lots

La « Préparation de plante » Dénomination, PER (Plant Extract Ratio : rapport entre le ratio final après extraction et avant tout autre ajout, et la quantité initiale de plante extraite, en rapport sec/sec s’il s’agit d’un extrait sec), DER (Drug Extract Ratio : rapport entre le ratio final de la préparation et la quantité initiale, donc après ajout des supports, …). Un produit sans support à un PER=DER. Parfois indiqué, le NER correspond au ratio natif. Composition intégrale (en %, avec la nature et l’objet de chaque constituant, critère de pureté des additifs - 231/2012) Standardisation (marqueurs, actifs) avec pour chacun la méthode d’analyse détaillée (validée ou non) et le standard utilisé comme référence Substances à surveiller : en référence à l’annexe I ou toute autre molécule susceptible d’être surveillée Impuretés (par exemple 2009/32), contaminants, contrôles bactériologiques,... cf. 1881/2006, 629/2008, 1259/2011, etc Attestations, autres données : données nutritionnelles, OGM (1829 et 1830/2003), Irradiations (1999/2, 1883/2003), Allergènes (2007/68, 2000/13), …), ... Stabilité avec indication du référentiel (ICH), besoin ou non d’homogénéisation, ... En outre, BECARRE Natural a complété le formulaire d’une préparation aux informations de sécurité alimentaire, même si une annexe III détaillée n’est pas exigible. à Niveau d’exposition : plante et extrait à Analyse de risque : toxicités, génotoxicité, mutagénicité, innocuité, NOAEL à Usage traditionnel trouvé à Evolution possible du process

Annexe III

Quelque-soient les cas de figure, l’intérêt est de garantir la sécurité aux consommateurs. L’Annexe III peut tout aussi bien correspondre à une ‘simple’ analyse bibliographiques des données toxicologiques connues et/ou effectuées par le producteur, qu’à une analyse poussées des risques Il est impossible de fournir une règle stricte applicable à tous les cas des très nombreux extraits de plantes existant, mais une bonne France : « list explication de votre position bien étayée de of plants, données (techniques, chimiques, réglemenother than taires) et en accord avec le texte publié ne mushrooms, au- peut que tomber dans une oreille réceptive. thorized in food Synadiet a travaillé à un guide ainsi qu’à une supplements and aide à la décision. their conditions of use »

L’arrêté plantes a été publié le 17 juillet 2014 : « Arrêté du 24 juin 2014 établissant la liste des plantes, autres que les champignons, autorisées dans les compléments alimentaires et les conditions de leur emploi ». Nous reprenons ici une lecture du texte notamment des annexes II et III Quels produits sont concernés par l’Annexe III ? L’annexe III est applicable dès lors que la préparation de plante n’a pas un usage existant et traditionnel suffisant pour justifier de la sécurité d’une préparation de plantes, tel qu’elle existe et existait. La traditionnalité porte donc sur l’extrait tel qu’il est connu ou défini – et il convient de démontrer que la préparation de plante est bien conforme à la préparation traditionnelle. Le texte de l’annexe III démontre qu’il est faux de penser qu’un extrait à l’eau ou à l’éthanol faible, ou avec un faible PER (alors que l’extrait dit traditionnel serait obtenu par une autre voie), permettrait de s’affranchir d’une Annexe III—l’inverse étant tout aussi vrai. Quelque soient les cas, l’analyse de risque doit être effectuée. Il est impossible de fournir une règle stricte applicable à tous extraits de plantes existant, mais une bonne explication de votre position bien étayée de données (techniques, chimiques, réglementaires) et en accord avec le texte publié ne peut que tomber dans une oreille réceptive. Synadiet a travaillé à un guide ainsi qu’à une aide à la décision.

Collecte des informations L’usage traditionnel peut être justifiée par différents moyens, dont les monographies, les rapports de ventes en quantités suffisamment significatives depuis - peutêtre - plus d’une ‘une génération’, le référencement dans des pharmacopées nationales (avec description spécifique de l’extrait et pas seulement de la plante), des références EMA, en rapport au WEU (Well established Use), voire des journaux de l’agence (cahiers n°3), etc… décrivant l’extrait (partie de plante, solvants et leurs concentration, process, ratio). L’annexe II permettra de confirmer que l’extrait de plante qui vous est proposé est bien conforme à l’extrait traditionnel. De l’analyse de risque à l’Annexe III : 3 cas La préparation est décrite dans la littérature, permettant de démontrer le recul d’utilisation et sa sécurité. Il suffit alors de l’indiquer. La préparation n’est pas décrite dans la littérature mais l’argumentaire démontre que l’extrait proposé est équivalent à une préparation décrite. Il convient d’apporter l’argumentaire permettant la comparaison à la préparation décrite. La préparation n’est pas décrite dans la littérature et aucune équivalence ne peut être démontrée : il convient d’apporter les analyses de sécurité - notamment toxicologiques - en rapport avec les dangers identifiés, à indiquer dans l’annexe III. BECARRE Natural - October 2020 - 41

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his is a non-exhaustive list of the number of decrees published by the European members, the European Parliament, the Council and Commission Regulations relating to food supplements. Each link of the chain from the early grower of the plants to the end-product must carefully check that its product fulfills the regulation and totally satisfy the safety needs.

Plant names Different countries, associations, working groups, etc... are working on the harmonization of plant and plant extracts in Europe, as for instance Synadiet, Federsalus, EHPM, etc... It works towards developing an appropriate regulatory framework throughout the EU for its members’ products. Synonyms accepted for plant The Plant List is a working list of all known plant species. It aims to be comprehensive for species of Vascular plant (flowering plants, conifers, ferns and their allies) and of Bryophytes (mosses and liverworts): see www.theplantlist.org/ Protection (Cites)

FOCUS HINTS

EC No 338/97 of 9 December 1996 on the protection of species of wild fauna and flora by regulating trade therein

Food supplements

Par t of the Regulator y Decrees

Contaminants

Other Quality specificities

General purposes

Allergen

EC 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs Toxins EU 1259/2011 of 2 December 2011 amending Regulation EC 1881/2006 as regards maximum levels for dioxins, dioxin-like PCBs and non dioxin-like PCBs in foodstuff - aflatoxin B1, total aflatoxins, Ochratoxin A, Patulin, Zearalenon, Fumonisin - and most of other toxins are in 1881/2006. PAH EU 2015/1933 of 27 October 2015 amending Regulation EC No 1881/2006 as regards maximum levels for polycyclic aromatic hydrocarbons in cocoa fibre, banana chips, food supplements, dried herbs and dried spice. Usually: à Benzo(a)pyrene ≤10 ppb à Sum of Benzo(a)pyrene, Benz(a) anthracene, Benzo(b) fluoranthene and Chrysene) ≤50 ppb Heavy Metals EC 629/2008 of 2 July 2008 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs (especially heavy metals). The limits vary depending on the origin and the use. However, we usually refer to : à Total Heavy metals : usually 10 ppm à Lead : usually 1 or 3 ppm (3 ppm in subsection 3.1.18) à Arsenic : usually 1.0 ppm à Cadmium : usually 1.0 ppm (3.0 for seaweed derivatives) à Mercury : usually 0.1 ppm Pesticides

Directive 2002/46/EC This main directive of 10 June 2002 on the approximation of the laws of the Member States relating to food supplements Infants, young children, special medical purposes, total diet replacement - also refers for substances to EU 609/2013 of the European Parliament and of the Council of 12 June 2013 on food intended for infants and young children, food for special medical purposes, and total diet replacement for weight control and repealing 92/52/EEC, 96/8/ EC, 1999/21/EC, 2006/125/EC and 2006/141/EC, 2009/39/EC of the European Parliament and of the Council and Commission Regulations (EC) No 41/2009 and (EC) No 953/2009. France: definition of “Compléments alimetnaires”, plants and Substances, 2011/329 and 2006/352 Organic EC 834/2007 and its implementing regulation of the European Commission 889/2008 gives the very strict rules for Organic products These regulations define rules at all stages of production, processing, labeling, control and import from third countries. 42 - BECARRE Natural - October 2020

EC 396/2005 of the European Parliament and of the Council of 23 February 2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending Council Directive 91/414/EEC + EC No 149/2008 of 29 January 2008 amending Regulation (EC) No 396/2005 of the European Parliament and of the Council by establishing Annexes II, III and IV setting maximum residue levels for products covered by Annex I thereto. This list is much more strict than the EP, and even more than the USP. ec.europa.eu/food/plant/pesticides/eu-pesticides-database/ for active substances and pesticides MRLs

Other limits Solvents and Residual solvents Directive EU 2016/1855 of 19 October 2016 amending 2009/32/EC on extraction solvents used in the production of foodstuffs and food ingredients, amending EC 2009/32 of 23 April 2009 on extraction solvents used in the production of foodstuffs and food ingredients. You may also refer in certain cases to Eur. Ph. 2.4.24 (methods) or 5.4 (limits).

EU 1169/2011 of 25 October 2011 amending EC 1924/2006 and EC 1925/2006, and repealing 87/250/EEC, 90/496/EEC, 1999/10/EC, 2000/13/ EC of the European Parliament and of the Council, Commission Directives 2002/67/EC and 2008/5/EC and Commission Regulation (EC) No 608/2004 Bacteriology Usually Eur. Ph. 5.1.8 (limits) and 2.6.12/2.6.31 (methods) GMO EC 1829/2003 of 22 September 2003 on genetically modified food and feed and EC 1830/2003 of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC. Nano technology / material Commission recommendation of 18 October 2011 on the definition of nanomaterial 2011/696/EU Novel Food Novel Food is defined as food that had not been consumed to a significant degree by humans in the EU before 15 May 1997, when the first Regulation on novel food came into force. ‘Novel Food’ can be newly developed, innovative food, food produced using new technologies and production processes, as well as food which is or has been traditionally eaten outside of the EU. The Novel Food Catalogue is a non-exhaustive list and serves as orientation. A plant may be not novel food when its extract is. EC 2002/46 of 10 June 2002, Substances Substances are more and more concerned in Europe, although each country may still have its own considerations, and although the definition of a substance is not fully clear yet. France publishes its Order regarding the use of other substances in supplements. It confirms that the use of substances other than those listed in Annex I, which lays down specific conditions of use for four ingredients, may be used in supplements provided they are not novel. NOR: ECFC1518714A on legifrance.gouv.fr

ektium (ex-PoliNat / Polifenoles Naturales) is the Spanish company dedicated to the identification, development and production of high quality active ingredients derived from plants, for use in the health-food and cosmetics industries.

Nektium (www.nektium.com) is located at Las Palmas, Canary Island, and focuses its efforts on the development and industrial production of high quality standardized products derived from plants, which are key ingredients in health food products. The company produces standardized extracts and novel ingredients and products, contributing to the prevention of diseases and improvement of quality of life through nutrition. Manufacturing high quality extract starts with the selection of qualified raw material suppliers. Rhodiola roots - for instance - are strictly harvested from the Altai region of Siberia as a result of several years of a collaborative efforts. Purity and sustainability are the two main selection criteria. Nektium stands for environmental preservation, sustainability and corporate responsibility. Production activities and facilities are ISO, HACCP & GMP certified. Organic raw material may be available. In 2018, Nektium has inaugurated the new Nektium facility, 10 000 m2 devoted to the new product development and production. www.nektium.com

ynadiet is the Food Supplement Association and supports the interests of the French food supplements industry at all levels of the legislative and regulatory process. SYNADIET is a member of relevant organizations : EHPM (European Federation), IADSA (International Federation) and EBF (European Botanical Forum). SYNADIET represents more than 250 members which most of them export worldwide. www.synadiet.org

noreal® is a firm created in 2006 which help nutrition and health manufacturer to be at the cutting edge of innovation by providing new ingredients, which demonstrated their efficacy. Supported by a meticulous sourcing, exclusive extraction process, clinical studies of safety and efficacy , Inoreal®’s products are formulated to allow innovation by having the agreement of international administrations, and are allowed for selling all across the world. www.inoreal.com www.satiereal.com www.elimreal.com

abinsa manufactures and markets phytonutrients and standardized herbal extracts, specialty & fine chemicals, and organic intermediates used in the nutritional, cosmetics, pharmaceutical, intermediary chemicals and food industries. Founded in 1988 by Dr. Muhammed Majeed, Ph.D., Sabinsa is a company dedicated to the principles of tradition, innovation and research. Sabinsa’s efforts have begun to earn the company vast recognition, in addition to growing sales. Sabinsa confirms everyday its position of leader on botanical extract, and at the origin of so many objectivized products (BioPerine from Black Pepper, Garcinia Cambodgia, Coleus Forslean, etc...). Sabinsa is a group which includes all the needed activities to provide high quality botanical extracts - and not only ayurvedic plants. Sabinsa has a research and development facility in India and near Princeton, NJ and six manufacturing facilities in and around Bangalore, India, and in Utah. Today, Sabinsa manufactures over 100 such plant-derived products, besides some synthetic and hemisynthetic products for perfumery. Sabinsa also provides custom manufacturing from lab scale to pilot / semi-commercial scale, and process development.

www.sabinsa.com www.sabinsacosmetics.com www.curcuminoids.com www.BioPerine.com www.boswellin.com www.selenoforce.com www.ursolicacid.com www.lactospore.com www.venocin.com www.glabridin.com www.seleniumselect.com

L ers n t r a p y t Quali

aboratoires ISOCELL is at the cutting edge of scientific research in the field of aging, and oxidative stress in particular. That’s how they developed GliSODin®, an oral grade of improved SOD. Based in Paris, Founded in 2001, ISOCELL has an international dimension, being present in over 30 countries. ISOCELL also hold several international patents (Europe, US, Japan) in these areas of research and development. Innovative products have been developed to strengthen the antioxidant and immune systems and protect the body at the cellular level against external stresses and aging. The products of the GliSODin® line are manufactured in accordance with Good Manufacturing Practices (GMP) www.glisodin.org www.glisodine.fr

www.glisodinskin.com

BECARRE Natural - October 2020 - 43

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plant extract is a substance or an active with desirable properties that is removed from the tissue of a plant, usually by treating it with a solvent, to be used for a particular purpose. Extracts may be used in various sectors of activities : Food and functional properties for foodstuffs (antioxidant, texturizer, etc…), Processing aids, additives – chemical replacers, pharmaceutical for therapeutic properties - preventive and/or curative – cosmetic for functional properties for beauty and well-being, etc… Some sectors of activities clearly define an extract. For cosmetic uses, for instance, a “cosmetic product” shall mean any substance or preparation intended to be placed in contact with the various external parts of the human body (epidermis, hair system, nails, lips and external genital organs) or with the teeth and the mucous membranes of the oral cavity with a view exclusively or mainly to cleaning them, perfuming them, changing their appearance and/or correcting body odors and/or protecting them or keeping them in good conditions. An extract may be common to different sectors based on its chemical description, although different sectors mean different technologies (different forms, solubility, stability), different regulation, different claim, different problematic. As representing manufacturers, we feel very concerned by the request of the end-users and may adapt its proposal. An extract must also respect : à The quality constraints in terms of irradiation, allergens, toxicity, adulteration, activity, stability, sustainable resources, traceability, etc… à The chemical and physical request in terms of description (TLC, physical recognition, genotype) as well as heavy metals, nitrates, solvents, residues such as pesticides or residual solvents, additives, foreign bodies, etc… à The regulation in terms of assay, sol-

W H AT I S A N E X T R A C T ? vent, environmental components, legal uses, registration or notification (reach, organic grades,...), labeling, claims, certification (GMP, ISO), etc…

Raw Material It’s important to understand that an extract starts from the raw material: the plants. The Identification of a good source includes the description of areas and type of collect, the capacity of collect, the use and not-used area and the impact of our needs for extraction to guarantee a sustainable source. It’s so important to analyze the supply chain starting from the collect, including the exporters and the different middle men, which may cause variability on the quality besides the natural variability linked to the weather and the soils. Finally, an overview of the needs of the local citizens and the risk (government, wars, weather, etc…) allows to find the right partners for the long term, and minimize the impact on the nature while checking the return to the people.

Preparation All equipment may not use the same materials, and the quality of the extraction may vary depending on the preparation of the raw materials. This goes from the cleaning to avoid foreign bodies to the cut or grinding. Dried herbals are more common, with the risk to put out some gaseous components and the benefit that the plant does not start to hydrolyze or produce non needed metabolites. Preparation may also consist in thawing (fruits may be frozen for instance), cooking (to produce aromas for instance), decoating, destemming, or even enzymatic reaction before the extraction.

Solvents A solvent must comply with the local regulation, be effective and be selective

enough (when needed). Most common are Water, Ethanol, Ethyl acetate, CO2, Methanol, Aceton, Acetic acid, Hexane… the choice of the solvent will impact on the yield of extraction (acquaintance with the targeted actives) in the full respect of the request from customers and environmental components (depletion of the ozone layer, life of the solvent in atmosphere, groundwater pollution, air pollution). On a chemical point of view, the best solvent may be chosen after looking at the dipolar moment : an electric dipole is a separation of positive and negative charges, characterized by their dipolar moment, a vector quantity (Coulomb. meter or Debye), subject to continuous electrostatic attraction and repulsion. Each solvent may be classified based on its dipolar moment (cf. : Ven der Waals Forces). à Apolar and low polar solvent, mainly lipophilic characteristic, with a moment from 0 to 1.5 (Hexane, cyclohexane, dimethoxymethane, Chloroform, Ethylic ether, phenol) à Polar aprotic solvent, mainly hydrophilic characteristic Protic solvent (a protic solvent is a solvent that has a hydrogen atom bound to an oxygen as in a hydroxyl group or a nitrogen as in an amine group). Polar protic solvents are solvents that share ion dissolving power with aprotic solvents but have an acidic hydrogen. These solvents generally have high dielectric constants and high polarity). Moment of most common solvents : Hexane (0), Ethanol (1.69), Methanol (1.70), Ethyl acetate (1.78), water (1.85), aceton (2.88). Depending on their miscibility, solvents may be used together (combined solvents, or separation from one solvent to another solvent).

regulation, effective and selective when needed. However, certain steps may require a long time of process.

Extraction process

Separation / Purification Different type of separation, based on size, weight, charge, hydrophilic / lipophilic capacity (separation liquid / liquid), solubil-

The process must generally be simple, fast, economic, in compliance with the local

Pressing : One of the more usual extraction (the morning orange juice), pressing may be used complementary or prior to other process, for instance to prepare the materials, defat it, etc…It causes mechanical perturbation and gives a liquid product. Distillation and hydro-distillation : By direct heating or steam, this process is mainly used for oils and volatile components. It may cause degradation (oxidation, hydrolysis, enzymatic reaction, ….) Solid / Liquid extraction : It consist in the extraction or separation of one active or more from solid materials working on the solubility in a liquid to obtain the soluble part and the insoluble part, or fractionation of an homogenous solution. The typical Solid / Liquid extractions are : Maceration : solid in solvent, room temperature Digestion : solid in solvent, over room temperature, below ebullition Decoction (=reflux): solid in solvent, temperature of ebullition infusion (teas) : solid in liquid at temperature of ebullition, then cooling of the suspension Elution, leaching (Lixiviation) : solvent goes through the solid Lixiviation : always with cold, fresh and new solvent Percolation : solvent goes on and through the solid

Concentration This step consists in the increase of the dry matter content to facilitate the further steps, using for instance evaporation.

ity, volatility (liquid / steam), etc… Filter press : the filter press uses increased pressure to maximize the rate of filtration and produce a final filter cake with a low water content. Membranes, Ultra filtration, Nano filtration : membrane filtration or cross-flow filtration technology which ranges between ultrafiltration (UF) and reverse osmosis (RO). The fundamental principle of Nanofiltration membrane technology is the use of pressure to separate soluble ions through a semi permeable material. Liquid / Liquid : Liquid-Liquid Extraction (LLE) is a method used in the recovery of a key component from a multi-component stream using an immiscible solvent. The two steams are contacted and separated. The solvent absorbs the key component stripping it from the original stream.

Crystallization Crystallization is the (natural or artificial) process of formation of solid crystals precipitating from a solution. Crystallization is also a chemical solid-liquid separation technique, in which mass transfer of a solute from the liquid solution to a pure solid crystalline phase occurs. The crystallization process consists of two major events, nucleation (the solute molecules dispersed in the solvent start to gather into clusters, on the nanometer scale) and crystal growth (subsequent growth of the nuclei that succeed in achieving the critical cluster size). Nucleation and growth continue to occur simultaneously while the supersaturation exists. à Single-solvent recrystallization : «compound A» and «impurity B» are dissolved in the smallest amount of hot solvent to fully dissolve the mixture, thus making a saturated solution. The solution is then allowed to cool => different solubility of compounds in solution drops => compound dropping (recrystallizing) from solution. The

slower the rate of cooling, the bigger the crystals formed. The solid crystals are collected by filtration. à Multi-solvent recrystallization : similar to the single-solvent but where two (or more) solvents are used. the proportion of first and second solvents is critical. Possible removal by distillation or by an applied vacuum. à Hot filtration-recrystallization : separate «compound A» from both «impurity B» and some «insoluble matter C». à Seeding : this can be spontaneous or can be done by adding a small amount

of the pure compound (a seed crystal)

Chromatography Chromatography is used for separation of mixtures. A mixture dissolved in a “mobile phase” passes through a stationary phase, which separates the analyte. The ultimate goal of chromatography is to separate different components. The stationary phase or adsorbent in column chromatography is a solid. The most common stationary phase for column chromatography is silica gel, followed by alumina. Also possible are ion exchange chromatography, reversedphase chromatography (RP), affinity chromatography or expanded bed adsorption (EBA). The mobile phase or eluent is either a pure solvent or a mixture of different solvents. It is chosen so that the retention factor. The Van Deemter equation in chromatography relates the variance per unit length of a separation column to the

linear mobile phase velocity by considering physical, kinetic, and thermodynamic properties of a separation à Column chromatography : the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open tubular column). Differences in rates of movement through the medium are calculated to different retention times of the sample. à Flash column chromatography : the solvent is driven through the column by applying positive pressure. This allowed most separations to be performed in less than 20 minutes, with improved separations à Thin layer chromatography (TLC) is similar to paper chromatography. However, instead of using a stationary phase of paper, it involves a stationary phase of a thin layer of adsorbent like silica gel, alumina, or cellulose on a flat, inert substrate. Compared to paper, it has the advantage of faster runs, better separations, and the choice between different adsorbents à Ion exchange chromatography uses ion exchange mechanism to separate analytes. It uses a charged stationary phase to separate charged compounds including amino acids, peptides, and proteins. In conventional methods the stationary phase is an ion exchange resin that carries charged functional groups which interact with oppositely charged groups of the compound to be retained. Ion exchange chromatography is commonly used to purify proteins using FPLC (fast protein liquid chromatography) à Chiral chromatography involves the separation of stereoisomers

HPLC (High Pressure Liquid Chromatography) and LPLC (low pressure). Preparative high performance liquid chromatography is

similar to analytical HPLC but features increased injected mass with possibility to “stack” injections automatically. There’s technically no product limitation, but usually expensive.

Semisynthesis Hemisynthesis or Semisynthesis or partial chemical synthesis is a type of chemical synthesis that uses compounds isolated from natural sources (e.g. plant material) as starting materials. These natural biomolecules are usually large and complex molecules. This is opposed to a total synthesis where large molecules are synthesized from a stepwise combination of small and cheap (petrochemical) building blocks. It is also possible that the semisynthetic derivative outperforms the original biomolecule itself with respect to potency, stability or safety. Drugs derived from natural sources are usually produced by harvesting the natural source or through semisynthetic methods.

Drying Drying means putting out the liquid. Dryers may be atmospheric (spray dryer, fluid bed dryer, etc...), or vacuum (belt dryer, tray dryer, etc…). Spray Dryer By definition, spray drying is the transformation of feed from a fluid state into a dried form by spraying the feed into a hot drying medium. The process is a one step continuous operation. The feed can be either a solution, suspension or a paste. The spray dried product conforms to powder consisting of single particles or agglomerates, depending upon the physical and chemical properties of the feed and the dryer design and operation Vacuum dryer At constant volume, when the pressure decreases, the boiling temperature decreases. Evaporation at a lower temperature makes possible to get a preserved product. In addition the high rate of heat efficiency is said to result in the best drying performance in the world. The drying

Adulteration & Falsification : get the extract you asked for Adulteration means falsification of an extract in terms of origin, assay, extraction, actives, or analysis, mostly in order to improve the price. à Method of analysis : • UV versus HPLC : except for very few actives / markers, UV methods always give much higher response than HPLC (when the standard exists of course) without being able to find a correlation. This may be versus HPLC, or can be many others, especially when using internal method. • Wave lengths : a different wave length or the use of a different Specific Absorption Coefficient • Standard : the use of a different standard for a better response by HPCL. • Family of the actives : refer to the global family of the actives instead of the active alone. • Reduction of the number of markers, what allows to use addition of chemical origin. The more there are markers in the description, the most difficult it is to falsify them. à Plant Extract Ratio : difficult to be sure to get a 4:1 extract when there’s no marker to control.

process is greatly accelerated due to the fact that the loaded material makes contact with the heating panel in the form of a thin layer. Vacuum drying does not cause the emission of any exhaust gases or hazardous smells. Lyophylization or cryodessication A lyophilizator needs to cool (for freezing and trapping water), create a vacuum (to sublimate water), generate heat and transfer heat energy to the product. Others : Rotary Drum Dryer : Wet material is introduced from one end of the dryer, and then stirred evenly by the oar of the inner barrel - Plate Dryer : kind of high-efficiency conductive and continuous drying - Blade

à NER / PER : voluntary misunderstanding between Native extract and Plant extract, sometime even considering the original plant, with the water. à Specie : use of a another bionomial origin, of even a plant close to the original plant (same genus) à Part of plant : use of a different part than the one mentioned or recommended (for instance, leaves instead of roots, even partly) à Addition of chemicals : addition of exogenous origin material, from other plants or from chemical process. They can be added to a colored carrier or sometimes to the plant powder in order to get at least a positive TLC. à Solvents : use of another solvent than the one mentioned for a more selective and efficient extraction. à Geographical Origin : plants may offer a different profile depending on the geographical origin, that you may need specifically. It can be also collected from a non-authorized zone. Those are the most common noted origins of adulterations.

Dryer : drying or cooling paste-like, granular, powdery and pulpous material - etc…

Before Packing Further steps before packing consist in Milling, compacting (to increase the bulk density for instance), grinding (to tune the size reduction in a safe way, especially with fragile particle or components) and then homogenization and standardization : those steps guarantee a optimized repartition and the correct and needed content of actives as mentioned in our CoA (certificate of analysis).

Personal Care

STANDARDIZED PLANT EXTRACTS

October 2020

Olepent®

Firming and repairing peptide Olepent® is the last innovation from Sabinsa, a skin firming and repairing peptide complexed with natural triterpenoid that has the potential to stimulate the skin’s natural production of collagen, restore structure of the skin and minimize the effect of degrading enzymes. The over activity of elastase and collagenase enzymes result in wrinkles, fine expression lines, thinning of skin, hyperpigmentation, dark circles of eyes and premature aging. The triterpenoid peptides have dual action for skin aging and collagen insufficiency. These act by inhibiting oxidation and excess activity of elastase and collagenases. Addition of peptides which augment collagen and other matrix protein, triterpenoid peptides provide complete protection against anti-aging. Similarly, oleanolic acid act as an effective agent in reducing the signs of aging due to oxidation, collagen insufficiency and over activity of enzymes like elastase and collagenase.

2 new studies SPF, Anti-Aging, Skin Brightening Two new studies have recently been published on the topical benefits of Sabinsa’s pTeroWhite® natural pterostilbene isolated and purified from Pterocarpus marsupium heartwood and formulated as a cream. The studies further the body of science into pTeroWhite®’s value as a cosmeceutical ingredient for anti-aging, skin brightening, and as a natural SPF agent (studies at 0.4% pTeroWhite® in a cream). Skin Brightening and Anti aging - 38 people, dermatological assessment, together with instrumental and image analysis. It improves skin brightness, elasticity, reduces the appearance of under-eye fine lines, wrinkles, and improves skin texture. Positive change at all time points in skin hydration and elasticity in both male and female subjects Sun Protection Factor - 12 and 25 people. In vivo SPF value of 6.2 ±1.30 (8.84 ±0.01 cream in vitro, and 21.73 ±0.06 as the ingredient pTeroWhite®). Further, the study demonstrated the potent antioxidant effects of pTeroWhite® in various in vitro and cellular test systems. The cream was found to be safe and nonirritant (25 people).

LactoSporin®: Novel Probiotic Metabolite For Skin and Hair

Centellin®

Sabinsa announces the introduction of LactoSporin®, an ingredient for cosmeceutical products that has anti-microbial, anti-oxidant, anti-inflammatory, and anti-collagenase properties, shows protective efficacy against UV radiation, environmental pollutants, and prevents microbial biofilm formation, induces hair growth, and prevents accelerated skin aging… LactoSporin® is prepared by a patented technology in a defined growth media. It is standardized preparation for antimicrobial activity with a broad spectrum of activity against gram-positive and gram-negative bacteria. LactoSporin® is an ingredient for cosmeceutical products that has antimicrobial, antioxidant, anti-inflammatory, and anti-collagenase properties, shows protective efficacy against UV radiation, environmental pollutants, and prevents microbial biofilm formation, induces hair growth, and prevents accelerated skin aging – so diverse exciting applications of LactoSporin®.

A new study published has evaluated the efficacy and safety of a formulation with Centellin® CG in a foot care cream (CCFC) in the management of cracked heels through cell culture and human clinical studies. It shows a beneficial effect on cell proliferation, collagen synthesis and significantly improves wound healing in the fibroblast scratch wound model. It also increased skin moisturizing. The findings demonstrate that CCFC helps in healing of cracks, reducing scaling and pain. None of the volunteers experienced any hypersensitivity reactions.

Glabridin 98 Skin lightening

NEWS

pTeroWhite®

We offer from 4% Glabridin licorice extract, to a purified product providing 98% Glabridin. Glabridin is renowned for its ability to inhibit tyrosinase and its anti-inflammatory properties, achieved through the inhibition of cyclo-oxygenase in the arachidonic acid cascade. The highest purified grade is standardized at ≥98% HPLC

Boswellin® Psoriasis

Centella extract and cracked feet

Cococin®

Fresh and Long moisturizer, Hair Cococin® is a tender coconut extract, produced by a unique lyophilisation process to ensure that the solids retain optimum biological activity. It is non-irritating to the skin and rich in nutrients that provide long-term moisturization and support blood circulation. “The Nourishment Factor”. Cococin® targets cell growth (in the hair follicle), as well as inhibiting 5-α reductase. It is recommended for use in regenerative skincare products and preparations, thanks to its cytokine and mineral concentrations. It also improves skin elasticity and tone and decrease roughness, while nurturing keratinous tissue to potentially reduce the appearance of the signs of aging.

Tetrapure®

Skin, Hair & Nails Sabisna’s Boswellia extract cream can be used to manage mild to moderate psoriasis through its anti-inflammatory effets. The cream with Boswellin from Sabinsa, Boswellia serrata Roxb extract standardized for 5% of 95% 3O Acetyl-11 Keto Beta Boswellic Acid (AKBBA) was studied in an open label, multi centered phase III clinical trial, evaluating 200 psoriatic patients with application of AKBBA cream three times daily for a period of 12 weeks. The researchers observed significant changes in LTB4 (p<0.001) & TNF alpha (p<0.01) values from the baseline along with significant changes (p<0.05) in VEGF & PGE2. Study : “Clinical evaluation of AKBBA in the management of psoriasis” 2 - BECARRE Natural - October 2020

TetraPure® is Natural anti-fungal remedy for Skin, Hair (anti-dandruff) & Nails. It’s a white to off white crystalline powder. compose of Tetrahydrocurcuminoides - as from Curcuma longa, but white. This product can be used as an antioxidant. TetraPure® shows great anti-mycosis and anti-dandruff activities. It completely inhibits the growth of dermatophytes Trichophyton rubrum, Microsporum gypseum and Epidermophyton flocossum. It enhances the activity against Malassezia furfur at all tested concentration. It reduces the colony counts of Candida albicans to less than 100cfu / ml. As other actives of the family of THC, Tetrapure shows tyrosinase inhibition as well as great inhibition of Melamin.

Topical products

Last News.......................................... 2 Why Becarre Natural......................... 3 Key Products..................................... 4 Plants list (Latin names)...................... 7 New : LactoSporin ............................ 8 New : Olepent ................................... 9 Skin Lightening ............................... 10 Comparison table sheet..................................11 Boswellin.......................................... 11 Two Ways Anti-Inflammatory Boswellin Rich AKBBA Boswellin and Psoriasis

Anti-acne.......................................... 12 Cosmoperine................................... 12 Enhancer of Actives and nutrients

Cococin............................................ 13 The Nourishment Factor

Rhodiola........................................... 14 Stress Support and Wrinkles

Foslean............................................ 15 Forskohlin Coleus (Plectranthus)

TetraPure.......................................... 15 Anti-fungal remedy for Skin, Hair & Nails

What means ‘extract’ ?................... 16 Process and terms

Our Partners.................................... 39 Reverse Side - Nutritional Last News 4 Why Becarre Natural 7 Products by properties 8 Key Products 11 Zynamite 18 Promond 20 Gugulipid 20 Tinofolin 21 Satiereal 22 Curcumin C3 Complex 23 Rhodiolife 24 Bacopa 25 Bromelain / Papain 26 Nigelllin 27 Ellagic Acid 27 GliSODin 28 enXtra 30

Starmeric 30 Boswellin 31 Pomegranate 32 Cococin 33 Elim’Real 33 Lactospore 34 BioPerine 35 Rhaponticum 36 Digezyme 36 Forslean 37 Selection of previous news 38 Plant Decree 39 L’Arrêté Plante 40 Some regulatory decrees 41 A selection of decrees 42 Our Quality Partners 43 What is an ‘extract’ ? 44

Becarre Natural represents, distributes and develops natural actives supported by sciences for nutrition, cosmeceutical and pharmaceutical purposes from manufacturers selected for their seriousness, quality and competencies. Becarre Natural acts as the centralized sales and marketing organization for selected manufacturers of plants extracts, either as your local contact or through appointed distributors who are then in direct contact with the manufacturers but followed up by Becarre Natural. Because industrials are more looking for reliability and know-how than an immeasurable list of products, Becarre Natural focuses on a specific origin of activities: standardized actives extracted from the nature under very strict process of production, with proven efficacy and sustainable resources. As per today, Becarre Natural represents by agreement different manufacturers, including Sabinsa Corp., Nektium, Isocell, Inoreal, … besides few others specific extracts from reputed sources. As the right note at the right time makes the music, the right product in the right formulation makes your success . ‘Becarre’ - that you may pronounce ‘be care’ - is the French word for the musical mark which cancels the alterations such sharp sign or flat signs. The English translation is ‘the natural sign’ .

BECARRE Natural 149 rue d’Estienne d’Orves F-92140 Clamart contact@becarre-natural.com www.becarre-natural.com

Gontran GAILLOT General Manager

Extracts for every facet

+33 [0]6 86 54 83 48 +33 [0]9 56 09 05 39 gontran.gaillot@becarre-natural.com gontran.gaillot www.becarre-natural.com becarre-natural BECARRE Natural - October 2020 - 3

KEY PRODUCTS Anti-inflammatory, Joints, Skin

Weight control, Satiety

Moisturizer, Hydration

Sun protection, Tanning

Antifungal, Antibacterial

Minerals, Synthetic, Intermediates

Skin, Hair, Nails

Lightening, Whitening

Antioxidant, ADN

Dark circles, Puffiness

PteroWhite® 90

Olepent®

Sun protection, Skin lightening

Unique patented pentapeptide : Firming and repairing

Source: Pterocarpus marsupium Inci : A Pterocarpus Marsupium Wood Extract Dose : 0.1-0.5% in creams / lotions / gels Bio-active ingredients: 90.0% Pterostilbene by HPLC

Inci : Oleanoyl sh-Pentapeptide-4 Off white powder, slighly hygroscopic

PteroWhite is standardized at 90% min. of pterostilbene, a molecule better absorbed, and more biologically active than resveratrol : antifungal properties are 5-10 times more powerful, inhibition of DNA synthesis, highest induction of PPARα, selective COX-2 inhibitory action, inhibition of melanogenesis and support of skin tone lightening and dyschromia management and protection of the skin from damage by ultraviolet radiation. Two new studies recently published : Skin Brightening and Anti aging : improves skin brightness, elasticity, reduces the appearance of under-eye fine lines, wrinkles, and improves skin texture Sun Protection Factor - 12 and 25 people. In vivo SPF value of 6.2 ±1.30 (8.84 ±0.01 cream in vitro, and 21.73 ±0.06 as the ingredient pTeroWhite®)

Peptides are the newest ingredients in anti-ageing skin care that are highly effective actives and are utilized in the most premium care products. Olepent® has a dual mechanism both as a firming and repairing peptide

Firming Peptide à Stimulated the skin’s natural production of collagen à Restores the structure of the skin and smoothens wrinkles à Minimizes the effect of degrading enzymes Repairing Peptide à Provides anti-inflammatory support à Helps in repairing skin barrier dysfunction à Repairs skin injury caused by wound or photo-damage

PERSONAL CARE

Tetrapure® LactoSporin®

Natural anti-fungal remedy for Skin, Hair (anti-dandruff) & Nails

Novel Probiotic Metabolite For Skin and Hair

Source: Vanillin and Acetylacetone Inci: Tetrahydrodiferuloylmethane Dose : 0.1-0.5% w/w (Skin Lightening), 0.15% w/w (Anti-fungal) Bio-active ingredients: 99% Tetrahydrocurcumin Research reveals that Tet-

Source: Bacillius Inci : Bacillus Ferment Filtrate Extract Antimicrobial activity : nlt 200 AU / ml against Micrococcus luteus Protein : nlt 1.0 w/v (Lowry’s Method) Pale yellow to brown color clear to hazy liquid with characteristic odor Launched on 2019, is an ingredient for cosmeceutical products that has anti-

microbial, antioxidant, anti-inflammatory, and anti-collagenase properties, shows protective efficacy against UV radiation, environmental pollutants, and prevents microbial biofilm formation, induces hair growth, and prevents accelerated skin aging – so diverse exciting applications of LactoSporin. Skin care : showed potent antioxidant, anti-inflammatory, anti-collagenase and UV protection potential, thus playing a protective role to the skin Hair Care : stimulated the anagen phase of follicular cell growth by increasing the proliferation of dermal papilla cells and by inhibiting 5a-reductase activity. Oral Care : significant reduction (for about 12 hours) in the growth, rate of pH drop (prevention from detrimental acidic environment) and glucan production was observed in S. mutans within 5 minutes of the LactoSporin

Glabridine 98% Skin whitening, Anti-inflammatory Source: Glycyrrhiza Glabra Inci : Glycyrrhiza Glabra (Licorice) Root Extract Dose : 0.05% - 1% depending on concentration of Glabridine Bio-active ingredients: 4% to 90% Glabridine (98% in partnership)

Glabridine is the main compound in the hydrophobic fraction of licorice extract. It is known for its beneficial effects on the skin due to its anti-inflammatory and antioxidant properties. In addition, Glabridine greatly inhibits melanogenesis. Some researchers have established that this effect may be due to the inhibition of tyrosinase activity, and Inhibition of the production of active oxygen species.

4 - BECARRE Natural - October 2020

rahydrocurcumin (THC) is one of the major metabolites of Curcumin. THC is reported to exhibit many of the same physiological and pharmacological actions as Curcumin, in some systems, better. It can be used safely (tests available) in shampoo, cream, lotion, spray or powder form. Anti-Fungal activity: TetraPure® shows anti-mycosis and anti-dandruff activities. à It completely inhibits the growth of dermatophytes Trichophyton rubrum, Microsporum gypseum and Epidermophyton flocossum à It enhances the activity against Malassezia furfur - all tested concentration à It reduces the colony counts of Candida albicans to less than 100cfu / ml. so... it’s also an anti-dandruff Tetrahydrocurcumin adapts a two pronged approach to the free radical onslaught (ORAC > 10 000 / g) à Prevention of free radical formation. à Intervention whereby already preformed radicals are quenched by THC. Skin Lightening : as other actives of the family of THC, Tetrapure shopws a tyrosinase inhibition of 1.8 IC50µg/ml - so much better than Kojic acid (7) or Vitamin C (9.33), as well as great inhibition of Melamin (better than Vit. C - 25 - or Kojic ro Arbutin - 100).

Venocin® 90 Blood Circulation, Wound Healing Effects, Anti-inflammatory Source: Aesculus hippocastanum Inci : Aesculus Hippocastanum Seed Extract Dose : 1-3.0% in creams / lotions / gels Bio-active ingredients: 90.0% Triterpene glycosides as Escin

Escin is a mixture of saponins that is known to help maintain healthy blood circulation and strengthen the capillaries and veins. Horse chestnut extract is clinically proven to be beneficial to people suffering from varicose veins, spider veins, hemorrhoids and related circulatory problems or “chronic venous insufficiency” (CVI)

SabiWhite®

Cococin®

Skin lightener, Evening skin tone, UVB protectant

Hair Growth, Moisturizer Restore the skin health in aged and damaged skin

Source: Curcuma longa Inci : Tetrahydrodiferuloylmethane Dose : 0.05-2.0% in creams / lotions Bio-active ingredients: 95% Tetrahydrocurcumin

Patented color-free natural extract derived from turmeric roots with proven luminosity boosting and skin tone lightening effects. A potent inhibitor of melanogenesis, and antioxidant protection benefiting skin tone, texture and integrity. A double blind placebo controlled clinical study with Fairness cream with 0.2% proved the significant decrease in melanin content.

Boswellin® Under eye, Puffiness, Night repair, Age-defying, Anti-ageing Psoriasis - Anti-inflammatory Source: Boswellia serrata gum Inci : Boswellia Serrata Resin Extract Dose : 0.5-2% Bio-active ingredients: AKBBA, b boswellic acids, >75% Boswellic acid

Anti-inflammatory : Boswellin¨ is a natural extract from the gum resin. Boswellin¨ contains boswellic acids that inhibit two pro-inflammatory enzymes 5-lipoxygenase and elastase, thereby supporting skin texture and integrity. Inhibiting. inflammation is known to be an effective approach to slow skin aging. Trials on 22 women, for 4 weeks There was a reduction of dark circles on application of the cream vs. application of placebo : A double blind placebo controlled clinical study was conducted on 21 healthy female subjects in the age group of 20-35 years. The subjects were provided with “Under Eye cream” containing 1% of Boswellin (and other natural ingredients) and a placebo, to be applied under each eye twice a day for a period of 4 weeks. The appearance of dark circles and puffiness was assessed by a dermatologist at predetermined time intervals A new clinical study of AKBBA in the management of psoriasis has been published in Clinical Dermatology 2014 : open label, multi centered phase III clinical trial, evaluating 200 psoriatic patients with application of AKBBA cream three times daily for a period of 12 weeks. This concerns a specific grade of Boswellia extract standardized for 90-95% of 3-O Acetyl-11 Keto Beta Boswellic Acid (AKBBA).

Bacopa Skin damage, premature wrinkles, age spots

Bacosides and the antioxidants help to dispel toxins epithelium layer, and improve skin complexion and stimulates skin cell regeneration. Brahmi - the other name - is used in treating of psoriasis, eczema, abscess and ulceration. Ayurvedic uses : Memory Enhancing, Anti-inflammatory, Analgesic, Antipyretic, Sedative, Anti-ulcerogenic, Anti-epileptic.

Helps restore the skin health in aged and damaged skin : Coconut water is rich in quinic acid and shikimic acid which acts as free radical scavenger and play important role in biosynthesis of polyphenols Augments new cell growth while promoting healthy tissues / hair : Cococin™ has been evaluated for its efficacy as a hair growth promoter, in comparison with Minoxidil as standard Helps in wound healing : Coconut water also contains cytokinins , which are also involved in healthy cell growth and differentiation for additional skin restoration Helps in skin hydration : It provides reservoir of vitamins, minerals, RNA phosphates and organic acids that support tissue health. Nutrition and reviving skin, and healthy tissue. Sabinsa has developed and patented Cococin + Saberry, synergistic formulation to promote healthy hair growth granted patent for «Protective Compositions for Dermal Papilla Cells».

Saberry® Water soluble skin lightening agent, Hair care Source: Emblica officinalis fruits (Amla) Inci : Emblica Officinalis Fruit Extract Dose : 0.2-1% w/w Bio-active ingredients: 10% b-Glucogallin, 50% gallates (tanins)

Amla extract helps protect the skin from the damaging effects of free radicals and heavy-metal induced oxidative stess. It also increases pro-collagen type I C-peptide and tissue inhibitor of metallo-proteinase 1 (TIMP-1) production, and decreases MMP-1 production, concomitant with elevated mitochondrial activity in the fibroblast, in a concentration-dependant manner. Skin Whitening : Skin lightening by inhibition of melanin formation, stronger melanin than ascorbic acid. Melanogenesis inhibitory activity against both MSH and cAMP induced melanogenesis at IC50 14μg/ml MMP-1 Inhibitor : Amla Extract has been reported to inhibit MMP-1 production from fibroblasts. Saberry also showed mild anti-tyrosinase activity compared to Kojic acid, using the mushroom tyrosinase activity assay (Saberry IC50 321μg/ml) ORAC hydro: 267 800 µmol TE/100g / Orac lipo: 400 µmol TE/100g HORAC: 34 500 µmol CAE/100g / NORAC: 90 00 µmol TE/100g SORAC: 10 200 µmol kunitsSODeq/100g / SOAC: 135 100 µmol VitE/100g UV Protector : Protects the fibroblast cells from UV radiation, with activity higher than ascorbic acid at same concentration. 5-alpha reductase inhibitory activity (about 80%)

Pomegranate Seed Oil Moisturizing, Nourishing, Elasticity, Eczema

Xymenynic Skin Firmer / Cellulite, Anti-inflammatory, Hair care Source: Santalum album seeds Inci : Xymenynic acid Bio-active ingredients: Xymenynic acid Dose : 0.2% - 0.5% (up to 1.5% in the oil phase)

Traditional used as a dermal application to treat skin lesions. Stimulates the microvasculokinetic activity of the scalp. Help preserve the integrity and texture of the skin (Hyaluronidase and Collagenase inhibition). Xymenynic acid improves the skin elasticity and minimizes the damage and imperfections linked to cellulite (orange peel aspect).

Source: Punica granatum – seeds Inci : Punica Granatum Seed Extract Dose : 0.1% - 1% Bio-active ingredients: Punicic acid ≥ 80% (CLnA)

Remarkable ability to nourish, moisturize and repair the epidermis and improve skin elasticity. Pomegranate seed oil is commonly used in cosmetic products to revitalize dull or mature skin, assist with wrinkles, and to soothe minor skin irritations, due to its unique composition of fatty acids, mainly punicic acid (CLA). The high content of conjugated fatty acids in pomegranate oil gives it strong anti-inflammatory properties and makes it a highly beneficial ingredient in formulations intended to reduce swelling and ease muscular aches and pains. Pomegranate oil can promote procollagen synthesis and strengthen the epidermis. It provides relief from minor skin irritations and inflammation, including dry skin, eczema, psoriasis and sunburned skin.

BECARRE Natural - October 2020 - 5

PERSONAL CARE

Source: Bacopa monniera Inci : Bacopa monniera Extract Bio-active ingredients: 20% and 50% total Bacosides (as A & B) Dose : 3 x 50 mg (children) to 3 x 100 mg (adult)

Source: Fresh tender coconuts Cocos Nucifera Inci : Cocos Nucifera (Coconut) Fruit Juice Dose : 0.5% - 2.0% Cococin is « The Nourishment Factor® » that provides a valuable nutrient pool for enhancing food and beverage, as well as cosmetic product formulations. Cococin can be safely used in oral care as well as lip care compositions.

Centellin® CG

Fucoxanthin [U-HPLC]

Wound healing, aged skin, dermal itch, cracked feet and heels

Antioxidant, Protection against UV induced damage in the skin, Antipigmentary activity

Source: Centella asiatica Inci : Centella Asiatica Extract Dose : 0.5-2.0% in soothing creams / lotions Bio-active ingredients: 40% Saponins Triterpenes

Well-known activity for wound healing, studies with Centellin CG showed an increase by 40% of the dermal fibroblast proliferation, and up to 20% collagen synthesis. Cracked feet : Recent studies published has evaluated the efficacy and safety of a formulation with Centellin® CG in a foot care cream, and demonstrated that it helps in healing of cracks, reducing scaling and pain, without any reports of hypersensitivity reactions (24 participants presenting cracks or fissure in feet, especially in the heel region).

Rhodiola Anti-ageing, Anti wrinkle, Sensitive, Sun

Source: Undaria Wakame Inci : Fucoxanthin / Laminaria Japonica Extract Dose : 0.05-1.5% Bio-active ingredients: Fucoxanthin UPLC, Fatty acids (PUFA)

The scavenging activity of fucoxanthin is almost 8 times higher than that by fucoxanthinol, and more than 13 times higher than that by alpha-tocopherol. Urikura et al. showed that fucoxanthin significantly suppressed ultraviolet B-induced epidermal hypertrophy, which may cause wrinkle formation, vascular endothelial growth factor, matrix metalloproteinases-13 expression, and the increase of thiobarbituric acid reactive substances (test irradiated cells and MMP expression). Topical treatment with fucoxanthin prevents skin photoage and wrinkle formation in ultraviolet B-irradiated hairless mice, possibly through the antioxidant and antiangiogenic effects of fucoxanthin : sunscreen for photoaging.

Pomegranate P40P Antioxidant, Anti-ageing, Lightening

Source: Rhodiola rosea – roots from Altai mountains under social responsibility program, more than 5 years aged – unique licence and partnership with Russia Inci : Rhodiola Rosea Root Extract Dose : 0.1%-0.3% Bio-active ingredients: Rosavins 3-15%, Salidroside 1-5%

Skin Barriers: Study (2008, «Journal of Cosmetic Dermatology“) showed that extracts of Rhodiola rosea improved the skin’s defensive barrier functions against the stress of ultra violet rays. Subjects of this study were classified as having sensitive skin. Elastin: increases components needed by the body to create elastin. Collagen: elevates the number of hydroxyproline and hexosamine molecules, needed to make collagen (dermis, 2nd layer). Antioxidant: pharmacological active compounds (esp. rosavins), which are able to assist the body systems with oxygen deprivation, stress and immune support - all important considerations for skin management.

Ursolic, Ursolate

Source: Punica granatum – mashed fruits Inci : Punica Granatum Extract Dose : 0.5% - 5% Bio-active ingredients: Total punicosides ≥ 40%, Punicalagins A+B ≥ 30%, Total polyphenols ≥ 50%

There are several cosmetic formulations that include an extract of pomegranate juice or oil. Pomegranate is rich in ingredients such as vitamins B5 and C, potassium, polyphenols etc., that enhance the beauty and life of skin. It is a good source of ellagic acid and antioxidants, which are helpful in destroying free radicals. It’s so a great remedy for skin that is damaged due to sun exposure or aging, to get smooth and youthful skin. Pomegranate is said to extend the life of fibroblasts, for the production of collagen and elastin. Collagen and elastin pump up the skin and render it elasticity. This in turn, keeps the skin youthful for years and minimizes the visibility of wrinkles. Natural pomegranate extract contains very few ellagic although it’s the usual mentioned assay, which is a metabolite by hydrolyzation of the punicalagin (1 punicalagin gives 2 ellagic acid). However, Ellagic acid has be studied orally for its activity as skin lightening.

PERSONAL CARE

Anti-ageing, Anti-wrinkle, Collagen build up Source: Salvia officinalis Inci : Salvia Officinalis (Sage) Leaf Extract Bio-active ingredients: Standardized in Ursolic acid or even ursolate (70%

Ursolic acid Na salt and up to 30% of Oleanolic acid Na salt), from Sage (Rosemary available). à Tissue repair, anti-inflammatory (resistant barrier on skin/ hair) à Collagen and elastin synthesis à Beneficial to the overall health and functions of photo-aged skin à Efficient inhibitor of Human Leukocyte Elastase à Hair care for conditioning and repair

...Nutricosmetics Beauty that reflects on the exterior

Hence, getting the “beauty from within” concept depends upong the integrity and functioning of various processes, inside and otuside.

Saberry: from the fruit of Emblica officinalis, with potent antioxidant capabilities (studies: melanogenesis inhibition, antioxidant, UV protection) Ellagic

Fucoidan Anti-ageing, Skin lightening, Skin protection Source: Laminaria japonica – whole plant Inci : Laminaria Japonica Extract Bio-active ingredients: Sulfo-Polysaccharides ≥ 40% (85% upon request)

Sulfated polysaccharide fractions of Fucoidan from Laminaria have shown potent antioxidant activity in in vitro tests. Cell viability and subsequent animal studies using Fucoidan as the test substance showed that both immune cells and mice were protected from the oxidative damage induce by ionizing radiation. Further testing in rats confirmed that polysaccharides from Laminaria japonica protected the male reproductive organs from radiation.

6 - BECARRE Natural - October 2020

acid: Polyphenol extracted from Punica granatum (studies: melanin formation, Hyperpigmentation management

Silbinol: pterostilbene from

Pterocarpus marsupium, as the pTeroWhite® (studies: suppressor of AGEs, safety)

Rosemary: from dried leaves of Ros-

marinus officinalis, rosmarinic and carnosid acids.

C3 Reduct:

colorless hydrogenated product from Curcuma longa (antiglycation, antioxidant)

...A selection for Hair care Cococin: Extract of tender coconut, in freeze-dried free flowing powder form (also available in liquid form for compatible solubility for skin and hair care formulations). Coconut water works at cellular level due concentrated amounts of Cytokinins (involved in the cell growth and differentiation for a perfect copy of its DNA). Cococin™ has been shown to promote the proliferation of fibroblasts (wound healing potential). It’s also a long term moisturizer.

• Cococin for hair care was compared in vitro on dermal papilla cells with

Minoxidil vs Minoxidil alone, with evaluation of “Human Hair Follicle Dermal Papilla Cell Proliferation Assay” and “5-α Reductase Inhibition Assay”. Minoxidil 5.0% + Cococin CG 0.25% was found to be the most potent for human hair follicle dermal cell proliferation (41.21%) as compared to a 5% Minoxidil solution (32.01%). And inhibition of 5α-reductase (14.88%) in comparison to 3.01% for 5% Minoxidil solution. • Sabinsa developed and patented a formulation based on Cococin and Saberry (Amla extract) to promote healthy hair growth

LastoSporin : Studies demonstrated that LactoSporin® stimulated the anagen phase of follicular cell growth by increasing the proliferation of dermal papilla cells and by inhibiting 5α-reductase activity.

Tetrapure:

TetraPure® shows great anti-mycosis and anti-dandruff activities. It completely inhibits the growth of dermatophytes Trichophyton rubrum, Microsporum gypseum and Epidermophyton flocossum. It enhances the activity against Malassezia furfur at all tested concentration. It reduces the colony counts of Candida albicans to less than 100cfu / ml. As other actives of the family of THC, Tetrapure shows tyrosinase inhibition as well as great inhibition of Melamin

Name

Common actives

Aesculus hippocastanum Amaranthus caudatus Andrographis paniculata Artocarpus lakkochaa Asparagus racemosus Bacopa monniera Berberis aristata Boswellia serrata Boswellia serrata Camellia sinensis Capsicum annuum Centella asiatica Citrus sinensis Cocos nucifera Coleus forskohlii Coleus forskohlii Oil Crataeva nurvala Curcuma longa Curcuma longa Eclipta alba Embelia Ribes Emblica officinalis Ginkgo biloba Glycine max Glycyrrhiza glabra Inula racemosa Kaempferia galanga Laminaria japonica Mangifera indica Melia azadirachta

Venocin Amaranthus Andrographis Artonox Shatavari Bacopin Berberine Boswellin Boswellia Oil Green Tea Paprika Centella Hesperidin Cococin Forslean Forslean Lupeol Curcumin Sabiwhite,THC Eclipta Embelia Amla Saberry Ginkgo Biloba Soy Glabridin Inula Galanga Fucoidan Mango Neemoids

beta Escin, Terpens Squalene Andrographolides Resveratrol - OxyResveratrol Saponins Bacosides Berberine Boswellic Acids, AKBBA, Polysacch. Oil Catechins, Polyphenols Capsaicin, Chilitenoids, Carotenoids Terpens, Asiaticosides, Saponins Hesperidin Carbohydrate, Minerals, Proteins Forskolin Oil, Pinenes Lupeol Turmerones Tetrahydrocurcuminoids Wedelolactone Embelin beta-Glucogallin Flavone, Terpens, Lactones Isoflavones Glabridin (4-98%) Lactones Cinnamate Sulfopolysaccharides, Fucoidan Mangiferin Limonoids

officinalis) enhances hair growth by stimulating the peripheral blood flow in the scalp and helps prevent alopecia and dandruff. Ursolic acid has been shown also to treat photo-aged skin (prevents the appearance of wrinkles and age spots) by restoring the collagen bundle structures and elasticity.

Xymenynic

acid from Santalum album : Hair vitality and reduce loss.

Topically it can be used for its anti-inflammatory properties in the management of arthritis, as well as helping to preserve the integrity and texture of the skin in anti-aging formulations. In hair care formulations, it improves hair vitality since it stimulates the microvasculokinetic activity of the scalp

Bacosides from Bacopa m. provides proper nutrients to the hair follicles thus invigorating the hair growth. It improves micro-circulation and hair growth. Its massaging is beneficial in checking dandruff, itchiness, formation of split ends and flakes

Eclipta alba is reported to improve hair growth and the black color of our hair

Plant

Name

Common actives

Momordica charantia Ocimum sanctum Olea europaea Phyllanthus amarus Piper nigrum

Momordica Tulsi Olive Phyllanthus Cosmoperine

Minerals

Chromium Picolinate, Seleno-Methionine, Methyl Selenocystein, Molybden Methionate, Gamma-Glutamyl-LSelenomethionine, N-Acetyl L-Cyst., etc…

Bitter Principles, Charantin Eugenol, Caryophyllene, Ursolic acid Oleanolic Acid, Oleuropein Bitter Principles, Phyllanthin, Tannins Tetrahydropiperine Policosanol, Coleus, Monolaurin, Plant Formula AcneZero Vitex Plant Formula Policoperine Policosanol, Oil, Piperine Plectranthus barbatus Forslean Forskolin Polygonum cuspidatum Resvenox Resveratrol, Stilbens Pterocarpus marsupium Pterowhite Tannins, Stilbens, Pterocarposide Punica granatum Pomegranate Ellagic Acid Punica granatum Pomegranate Punicosides, Punicalagins, Polyph. Punica granatum Pomegranate Punicic acid CLnA Quercus infectoria Gallnut Polyphenols Rhodiola rosea Rhodiolife Rosavins, Salidroside Rosmarinus officinalis Rosemary Carnosic,Ursolic,Rosmarinic,Betulinic Saccharum officinarum Policosanol Policosanol Salvia officinalis Sage Ursolic Acid, Na Ursolate Santalum album Xymenynic Xymenynic Acid Sapindus trifoliatus Sapindin Saponins Serenoa repens Saw Palmetto Fatty Acids Sesamum indicum Sesamin Sesamin Synthesis Alpha Lipoic, 1,2-Hexanediol, Octanediol, Decanediol Terminalia Terminalia Arjunolic, Saponins, Tannns, Ellagic Undaria pinnatifida Fucoxanthin Fucoxanthin Vitex agnus castus Vitex Artemetin Vitex negungo Vitex Agnuside Withania somnifera Ashwagandha Withanolides, Alkaloids, Withaferin Zingiber officinale Ginger Gingerols

BECARRE Natural - October 2020 - 7

PERSONAL CARE

Plant

Ursolic and Ursolate: Ursolic acid (from Salvia officinalis or Rosmarinus

FOCUS Novel Probiotic M etabolite

actoSporin® is a non-living microbial cell product having biological activity. It is purified from the culture supernatant of probiotic strain, B. coagulans MTCC 5856 (LactoSpore®) with an INCI name - Bacillus Ferment Filtrate Extract.

Skin microbiome

The normal human skin is a microbiome colonized by 10,000 – 1,000,000 beneficial microorganisms, especially bacterial units/ cm2 that prevent the growth of pathogenic organisms and maintain the immunity of the skin and nourish the skin optimally with an established safety profile. Skin microbiome protect the skin by : à They act as a protective shield by preventing pathogenic microorganisms from provoking an immune reaction à They produce antimicrobial metabolites to combat pathogenic bacteria à They boost the skin’s functionality in dealing with external damaging factors including free radicals, sun, and pollution à They help the skin stay hydrated à Restore a healthy pH balance in the skin These functions creates a calming environment for the skin, help to reduce inflammation, flare ups and prevents premature aging.

FOCUS HINTS

Next level postbiotics

These are the water soluble, non-living microbial cell products, fractions, lysates or metabolites obtained from microorganisms possessing biological activity (Patel and Denning 2013). It has various advantages over probiotics (Aguilar-Toalá et al., 2018). à Biological activity in non-viable state à Lower chance for microbial translocation à Lower chance for microbial infection à Improved inflammatory defence à Favourable physiochemical properties (solubility) à Favorable pharmacokinetic properties (absorption, distribution, metabolism, and excretion)

LactoSporin® à A standardized antimicrobial product à It’s an extracellular protein metabolite obtained from the supernatant of probiotic strain, B. coagulans MTCC 5856 (LactoSpore®) à Pale yellow to brown color clear to hazy liquid with characteristic odor à Completely soluble in water à It acts against other pathological organisms à It can be a potent next-generation probiotic in skin care application. Thus, eliminating the usage of live microorganisms in various biological streams à Patents EP : “Method of producing partially purified extracellular metabolite products from Bacillus coagulans and biological applications thereof” 8 - BECARRE Natural - October 2020

Anti-aging effects of LactoSporin® Anti-collagenase activity : UV radiation induces the collagen degradation by enhancing the activity of collagenases, a type of matrix metalloproteases (MMPs) (Sanches Silveira and Myaki Pedroso 2014). LactoSporin® inhibited the collagenase activity (percentage inhibition) in a dose-dependent manner with an IC50 of 1.8% v/v. Thus, LactoSporin® can preserve the extracellular matrix of the skin and prevent wrinkle formation and skin sagging. Antioxidant activity : Reactive oxygen species (ROS), the major contributor for ageing is generated from mitochondria (Rinnerthaler et al., 2015). It acts as the major factor in developing the oxidative stress that exceeds the antioxidant defence of the target cell. UV radiations are the major extrinsic factor that generates the ROS leading to sun-induced skin ageing (photo-ageing) (Poljšak and Dahmane 2012; Xu et al., 2017).

LactoSporin ®

for

Skin, Hair

and

Oral Hygiene

LactoSporin® is a non-living microbial cell product having biological activity : Antimicrobial activity, antiageing effects and hair growth promotion Beneficial effects of LactoSporin® LactoSporin® has wide applicability starting from oral health to UV protection, anti-collagenase, and antiinflammatory:

LactoSporin® showed dose-dependent DPPH scavenging effect with an IC50 of 0.43%. This result emphasises the role of LactoSporin® as a potent antioxidant in cosmetic preparations. Anti-glycation effect : Glycation is the process of undesired cross-linking of collagen proteins with sugar molecules, eventually impairing the function of collagen fibres, making the skin hard and aged in appearance (Chaudhuri et al., 2018). The anti-glycation effect of LactoSporin® was performed using ribose sugar and bovine serum albumin. LactoSporin® was found to inhibit the formation of AGEs by 50% with an IC50 of 0.7% v/v. Thus, inhibition of AGE formation by the retardation of the collagen fibres glycation provides the skin with youthful and aesthetically pleasant appearance (83% Inhibition at 2%, 56% @1%, 36% @0.5%) UV protection effect : Humans are mostly affected by UV-A followed by UV-B. UV-B being shorter in wavelength and higher power radiation can easily penetrates deeper into the tissues of the skin. Thus, causing structural damage to the DNA and induce apoptosis. Whereas, UV-A radiation is mostly responsible for the generation of ROS such as superoxide anion, hydrogen peroxide and singlet oxygen, causing ROS-mediated inflammatory skin damage (Sanches Silveira and Myaki Pedroso 2014). The phototoxic (UV-A and UV-B light-induced toxicity) preventive effects of LactoSporin® was studied using mouse fibroblast cell line (Balb/C 3T3). LactoSporin® showed notable protection from UV-A as well as UV-B-induced cell death (protection against UV radiation-induced toxicity). Anti-inflammatory effect : LactoSporin® tested at various concentrations showed a dosedependent anti-inflammation activity, so potentiality of LactoSporin® against the skin inflammation induced by pro-inflammatory environmental insults (inhibition of Lipopolysaccharide induced secretion, TNF-α on human cell line THP-1.

Hair growth promotion with LactoSporin® Hair is one of the fastest growing tissues of the human body. Hair follicles undergo repetitive regenerative cycles consisting of anagen (rapid growth, active stage), catagen (regression, physiological involution stage), and telogen (resting stage). Hair proliferation is controlled by androgen hormones (e.g. testosterone). Androgens shorten the anagen phase by targeting dermal papilla cells (DPCs) of the hair follicle. The 5α-dihydrotestosterone (potent active metabolite generated by 5α-reductase) has greater affinity towards androgen receptors in DPCs. Thus, extending the anagen phase is an effective treatment for the prevention of hair loss. LactoSporin® capability of hair growth promotion was assessed by estimating the proliferation of Human Follicle Dermal Papilla cells (HFDPC) in vitro. LactoSporin® induced 29.7% proliferation of dermal papilla cells and 16.2% inhibition of 5α-reductase activity in vitro at 0.5% v/v LactoSporin® concentration. (picture : proliferation of dermal papilla

cells with LactoSporin®)

Hair growth potential of LactoSporin®

% Increase in Dermal Papilla Cell Proliferation (conc. v/v) 25 20 15 10 5 0

0.5 %

0.25 %

0.125 %

FOCUS

Olepent® Skin Firming and repairing peptide

Olepent™ is a innovative and unique patented pentapeptide complexed with oleanolic acid, with dual mechanism

Firming Peptide

• Stimulated the skin’s natural production of collagen

• Restores the structure of the skin and smoothens

wrinkles

eptides are the newest ingredient in anti-ageing skin care that are highly effective actives and are utilized in the most premium skin care products.

• Minimizes the effect of degrading enzymes

Olepent® has a dual mechanism both as firming and repairing peptide.

Repairing Peptide

• Provides anti-inflammatory support • Helps in repairing skin barrier dysfunction • Repairs skin injury caused by wound or photo-

Skin Care The triterpenoid group in Olepent® acts by preventing oxidation and excess activity of serine proteases like elastase and collagenase that result in the wrinkling of skin.

damage

Olepent has a significantly better anti-ageing potential like elastase inhibition, collagenase inhibition, Transforming Growth Factor beta (TGF-β) increase, Fibroblast Growth Factor (FGF) increase and antioxidant activity which makes it a promising ingredient in the segment of anti-ageing. ®

INCI Name : Oleanoyl sh-Pentapeptide-4 Description : Off-white powder, slighkly hygroscopic Purity Oleanyl Pentapeptide (HPLC) ≥90% w/w Shelf-life : 2 years

LactoSporin (...

60%

80% Elastase Inhibtion

Increase elasticity

80% Elastase Inhibition

Increase elasticity

90% TGF-β Increase

Rebuilds collagen

86% Collagenase Inhibition

Repairs wrinkles

40% FGF Increase

Repairs skin/reduces scarring

30% Antioxidant Potential

More luminous complex to skin

% Protection from UV Exposure Lactosporin® v/v

UV-A

UV-B

50% 40%

continued)

30% 20%

Antimicrobial activity

Oral Hygiene

According to Riazi et al., 2012, LactoSporin® might exert antimicrobial action by targeting the plasma membrane of sensitive microbial cells and forming transient pores followed by leakage of intracellular contents and dissipation of pH gradient component of proton motive force (PMF). The PMF is important for physiological processes such as ATP generation, ion transport as well as motility, thus affecting the sustenance of the organism.

The surface of the tooth is covered by a biofilm of microorganism, especially bacteria. Tooth decay or dental caries results from the acids secreted by certain bacteria. Streptococcus mutans are the common type of acid-producing oral pathogen that is present mainly in the mouth, pharynx and intestine. It decays the tooth by secreting glucose polysaccharides known as glucans (Forssten et al., 2010).

Results : LactoSporin® was found to be highly active against the important skin pathogens such as Staphylococcus aureus, Streptococcus epidermis, acne-causing bacterium – Propionibacterium acne, as well as against an opportunistic pathogen – Pseudomonas aeurginosa.

LactoSporin® was evaluated for the effect of oral hygiene by assessing its impact on the growth and drop in pH level of S. mutans.

It exhibits a broad spectrum antimicrobial activity against both Gram-positive and Gramnegative bacteria. The minimum inhibitory concentration (MIC) against various pathogenic groups was found to be in the range of 0.5 to 4.0% v/v. LactoSporin® was effective in killing the tested harmful bacteria at varied pH (2-5) and temperature ranges (70 – 90 °C). Also, LactoSporin® retained the antimicrobial efficacy in varied storage conditions (Stable & Accelerated condition).

10% 0%

Biofilm, the extracellular matrix on living or non-living surfaces are less susceptible to antimicrobial agents. Thus, these associations of microbial cells are difficult to target and treat (Donlan 2000). LactoSporin® exhibited a concentration-dependent biofilm inhibitory effect against S. mutans, S. aureus, P. aeruginosa and E. Coli (broth micro-dilution method).

0,25 %

0,125 %

Broad-spectrum antimicrobial efficacy MIC of Lactosporin® (%, v/v) 4,5% 4,0% 3,5% 3,0% 2,5% 2,0% 1,5% 1,0% 0,5% 0,0%

A significant reduction (for about 12 hours) in the growth, rate of pH drop (prevention from detrimental acidic environment) and glucan production was observed in S. mutans within 5 minutes of the LactoSporin® treatment. Biofilm inhibitory potential of LactoSporin®

0.50 %

Anti-aging effects of LactoSporin® Inhibition, %DPPH scavenging at different conc. 100 80 60 40 20 0

Collagenase

Glycation 2%

0.5 %

DPPH 0.25 %

Inflammation

0.125 %

BECARRE Natural - October 2020 - 9

FOCUS

wo forms of melanin are produced in the epidermis: phenomelanin, which is red to yellow in color, and eumelanin which is dark brown to black. The relative proportions of these also influence skin color. In addition, individuals differ in the number and size of melanin particles. Skin pigmentation is influenced by several factors like: à Hemoglobin in the blood vessels à Carotenoids in the dermis à Particularly, the dark pigment, melanin in the epidermis.

Production of Melanin Melanin biosynthesis (melanogenesis) is influenced by genetics, environmental factors, diet and medication. The production of melanin by specialized cells called melanocytes (in the basal layer of the epidermis in light skinned people and in the basal as well as horny layer in dark skinned people) occurs through the action of the enzyme tyrosinase. The rate-limiting step in melanogenesis is the conversion of L-tyrosinase to melanin, through the action of tyrosinase. Copper and oxygen act as catalysts. Other enzymes also control melanin production, particularly in the presence of sulfur. These include the following: à Dopachrome oxidoreductase which controls melanogenesis in the absence of tyrosinase. It helps to convert dopachrome into 5, 6-dihydroxyindole.

FOCUS HINTS

à Alpha-glutamyl transpepsidase which helps to maintain the balance in the biosynthesis of eumelanin and pheomelanin. Variation in skin pigmentation is attributed to the levels of melanin produced and the number of melanocytes present. Although light skinned and dark skinned people may have the same number of melanocytes present, the rate of melanin production is greater in darker skin tones. Additionally, the melanin present in the epidermal layers of darker skins is resistant to enzymatic degradation. Increased production of melanin on one side of the skin and dramatically reduced decomposition of melanin on the other side results in darker skin tones, in light skinned people. Melanin granules synthesized in the melanocytes are then transferred from the cytoplasm of the melanocytes to the basal cytoplasm of the keratinocytes. They thus form a protective covering in the inner layers of the epidermis, absorbing UV rays and inhibiting their penetration.

Controlling Melanin Synthesis Various types of inflammatory mediators such as leukotrienes and prostaglandins, cytokines and growth factors may influence melanin synthesis by affecting the proliferation and functioning of melanocytes. This explains why inflammatory diseases often induce hypopigmentation or hyperpigmentation. The enzyme, protein kinase C that phosphorylates proteins may also influence the growth and differentiation of melanocytes. 10 - BECARRE Natural - October 2020

Sk in Lightening Cytokines such as endothelins (also known as vasoconstrictive peptides) are also reported to accelerate melanogenesis.

Quick view of our Main Products à TetraPure and Sabiwhite (tetrahydrocurcuminoids) : anti-tyrosinase activity along with High ORAC value, and Inhibition of Melanogenesis à Glabridine (from 4% to 98% from licorice): the well-known whitening agent à pTeroWhite®:new studies on pterostilbene purified from Pterocarpus marsupium à Saberry (β-glucogallin from Amla) : white water soluble Skin Whitening, MMP-1 Inhibitor, UV Protector à OxyResveratrol Artonox: strong and dose dependent inhibition of Tyrosinase enzyme activity and melanogenesis inhibitory activity à Ellagic Acid (pomegranate), lightening, UV protection, also in oral form à Fucoxanthine (wakame) : lessened UVBinduced epidermal hypertrophy, VEGF, and MMP-13 expression à Soy extract : lightening action in solar lentigenes (hyperpigmentation due to sun)

Skin lightening cosmeceuticals The toxicity associated with hydroquinone use, induced researchers to identify less dangerous botanicals with comparable activity. The general modes of action include inhibition of the formation of melanosomes, inhibition of tyrosinase biosynthesis, and inhibition of melanin biosynthesis and interference of the transfer of melanosomes into the keratinocytes. Some agents also have a chemical effect on melanin with an increase in the degradation of melanosomes in the keratinocytes. Antioxidants such as ascorbic acid and others help to decompose preformed melanin. Hyperpigmentation due to UVA and UVB damage may also be addressed by preventive measures using antioxidant compounds with sunscreen effect and free radical scavenging action. Research efforts are generally aimed at achieving one or more of the following effects:

Artonox™ With Tetrapure® and Glabridin 98%, Artonox™ is a new comer in our range for skin brightening. Obtained from the dried wood of Artocarpus lakoocha, it provides 95% of Oxyresveratrol, an analog of Resveratrol (a stilbenol 2’,3,4’,5-tetrahydroxystilbene). Oxyresveratrol exhibits bout 150 fold more potent skin lightening inhibitory effect than Resveratrol (3,4’,5-trihydroxystilbene), and 32 more than Kojic. Artonox™ is dedicated for: à Skin lightening formulations à Antioxidant à Anti-aging formulations

The concept of skin lightening is not limited to decreasing melanin in skin but also encircles age spots, Melasma, Chloasma, Freckles, Post inflammatory hyperpigmentation Sun induced pigmented blemishes.

pTeroWhite® Two new studies recently published: • An Open-Label Single-Arm, Monocentric Study Assessing the Efficacy and Safety of pTwroWhite® for Skin Brightening and Antiaging Effects: significant improvement in under-eye fine lines at all-time points and in wrinkles, frown lines, and crow’s feet area at the end of the study. The pterostilbene preparation was able to improve the skin luminosity with continuous application. • A second clinical study, a randomized study to determine the Sun Protection Factor of pTeroWhite® demonstrated the effect as a natural SPF ingredient, the potent antioxidant effects in various in vitro and cellular test systems, and the safety.

à Regulation/inhibition of tyrosinase, dopachrome oxidoreductase and dopachrome tautomerase involved in melanogenesis à Regulation of cytokine network including endothelin à Regulation of genes related to melanogenesis à Combinations of the above approaches

Skin Brightening When Skin Needs Brightening Optical properties modulate the appearance of skin. Skin needs brightening when: à Too transparent or too highly pigmented skin appears spotted and unhealthy. à When skin is dull. Dull skin is dehydrated skin which is to transparent or hyper pigmented. à Early aged skin is also dull and rough due to loss of surface integrity.

What Skin Brightening Cosmeceuticals do The effects of skin brighteners are: à Radiant complexion. à Beautiful skin glow. à Even skin tone, without freckles, age spots or other types of discoloration. à Rejuvenated, nourished and smooth skin

Other related products Tyrosinase inhibitors such as Arbutin (from the leaves of the common bearberry), Arctophylos urva ursi and other plants, Glabridin from licorice (Glycyrrhiza glabra roots), ascorbic acid and its derivatives, Kojic acid (a bacterial carbohydrate metabolite) are better tolerated than hydroquinone. Aloesin from Aloe is reported to be a non-competitive inhibitor of

FOCUS

Rich AKBBA Boswellin and Psoriasis A new study on one of the most famous and powerful anti-inflammatory. Boswellic acids - and especially AKBBA - have been found to inhibit 5-lipoxygenase by one of two ways on 5-LO as well as (HLE) and psoriasis

Management of psoriasis A study on Sabinsa’s Boswellia cream for treatment of psoriasis, Clinical Evaluation of AKBBA in the management of psoriasis, was published in Clinical Dermatology 2014; 2 (1): 17--‐24. US clinical trials, intended to have AKBBA eventually approved as a drug by FDA for treatment of psoriasis, will begin in the near future. This will be a significant step for a nutraceutical company in USA.

Mean scores of arthritic symptoms as evaluated in an open field study of boswellic acids-containing topical analgesic Chilisin® (TM of Sabinsa Corp). In addition, a four week study as well as a three month toxicity study showed that administration of boswellic acids at 5 to 10 times the ED50 value did not cause side effects.

Inhibition of Leukotriene Synthesis The presence of a specific AKBBA-binding site on 5-LO that is distinct from the arachidonate substrate-binding site was determined using photo affinity labeling. Among the several compounds classified as leukotriene synthesis inhibitors, nonredox inhibitors, such as boswellic acids, are preferred. Unlike redox type inhibitors they do not interact with other biological redox systems, lessening the likelihood of side-effects like methaemoglobin formation. AKBBA has been identified as the only leukotriene synthesis inhibitor so far that inhibits 5-LO activity by noncompetitive, nonredox mechanisms (35% of topical formulation as cream, lotion or gel)

The cream of Boswellia serrata Roxb extract standardized for 5% of 95% 3‐O‐Acetyl-11‐ Keto Beta Boswellic Acid (AKBBA) was studied in an open label, multi centered phase III clinical trial, evaluating 200 psoriatic patients with application of AKBBA cream three times daily for a period of 12 weeks. The researchers observed significant changes in LTB4 (p<0.001) & TNF alpha (p<0.01) values from the baseline along with significant changes (p<0.05) in VEGF & PGE2. Reduction in ‘modified’ PASI score from the baseline visit was in consensus with the global evaluations by physician and patients. The evidence shows superior anti-inflammatory responses rates, demonstrating that this non‐toxic treatment is beneficial for those suffering from this condition.

oxidation chain reaction from tyrosine/DOPA to melanin. Our products are usually compared to Arbutin and to Kojic acid.

Arbutin and Kojic acid inhibit tyrosinase directly, while L-ascorbic acid and its derivatives are believed to act as reducing agents on intermediates in melanin biosynthesis at various points in the

Green tea is also reported to be a competitive tyrosinase inhibitor through the gallocatechin moiety in the major catechin constituents epicatechin gallate, epigallocatechin gallate and gallocatechin

Orac

“Our understanding of this ingredient, originating from one of the earliest compounds released by Sabinsa, called Boswellin®, is growing, and we want the world to know the positive research and the impact it has on human health,” said Sabinsa founder Dr. Muhammed Majeed. “Such indications prevalent in Ayurvedic texts are now being proven by modern research, in terms of correctly standardizing, isolating the unique compound(s) and ultimately formulating safe and effective products.” The cream product is sold and marketed as a drug in India. Sabinsa holds several patents for this and other uses of Boswellia. Clinical evaluation of AKBBA in the management of psoriasis, Muhammed M., Nagabhushanam K., Sankaran N., Sood R., Karri S.K.

Although far from the scope of a dietary supplement, the exploratory process that has gone into such a traditional ingredient showcases Sabinsa’s passion for applying modern day technology to

tyrosinase, affecting (...)(...) the action of tyrosinase complex in the substratum and reducing the conversion of DOPA into melanin.

Comparative table sheet

traditional botanicals to help create natural solutions for health.

gallate. Paper mulberry extract (from the root bark of Broussonetia kazinoki x B. papyrifera ) also contains active depigmenting agents, which were shown to be more efficacious than hydroquinone (IC 50 of 2.5 mg/ml against 5.5 mg/ml for hydroquinone).

DPPH

Tyrosinase

Melanin

Elastase

Collagenase UV Protection

Artonox

19 735

2,700

0,049

12,000

120,000

92,000

n.c.

Glabridine 40%

3 256

49,000

0,250

3,000

n.c.

Pterowhite

12 508

4,900

6,900

0,550

n.c.

Resveratrol

25 223

3,010

5,500

2,500

n.c.

Saberry

2 682

n.c.

321,000

14,000

n.c.

15 [UVA] 42 [UVB]

Sabiwhite

10 786

n.c.

1,770

3,000

n.c.

Tetrapure

10 212

1,300

1,800

3,200

n.c.

Kojic Acid

500,000

7,000

100,000

n.c.

Vitamin C

3 400

1,930

9,330

25,000

n.c.

500,000

193,600

100,000

n.c.

Arbutin

à ORAC Value : μmol Trolox equivalents/g (Higher is greater) à DPPH Inhibition: IC50μg/ml (lower is greater) à Tyrosinase Inhibition: IC50μg/ml (lower is greater)

à à à à

Melanin Inhibition: IC50μg/ml (lower is greater) Elastase Inhibition: IC50μg/ml (lower is greater) Collagenase Inhibition: IC50μg/ml (lower is greater) UV Protection: IC50μg/ml (lower is greater) BECARRE Natural - October 2020 - 11

FOCUS

cne is a common skin disorder which is a visible end result of hormonal, bacterial and inflammatory disturbances that take place at the level of the oil pore (pilosebaceous follicle). It is characterized by presence of open comedones (black heads) and closed comedones (microcysts).

Some of the common causes of acne à Growth and multiplication of acne causing bacterium Propionibacterium acnes. à Higher levels of the hormone Dihydrotestosterone by the activity of the enzyme 5-alpha reductase. à Increase in androgens during puberty and adolescence which in turn, triggers an enlargement of the sebaceous glands.

Anti-Acne A unique formulation to fight the various form of acne and work on the sebum control

At concentrations above 0.4%, it effectively inhibits the P. acne bacteria and the activity is well comparable to 1% Clindamycin gel.

Anti-microbial Coleus oil Coleus oil is an oil obtained from the fresh roots of Coleus forskohlii. It is standardized to 15% Bornyl acetate and 15% Decanal. The oil having good anti-microbial activity is a potent anti-acne ingredient. Coleus oil was found to effectively inhibit the growth of skin pathogens such as: à Propionibacterium acnes (associated with acne) : Inhibition of more than 25% at 1.25µg/ml (versus 15% for tea tree oil), and still 15% at 0,5 mg/ml

Vitex oil is extracted from the berries of Vitex agnuscastus and is standardized for 0.40% of Artemetin

Prevention of acne

Coleus oil is obtained from the fresh roots of Coleus forskohlii standardized to 15% Bornyl acetate and 15% Decanal.

Acne may be prevented by: à Reducing sebum production (secreted by sebaceous glands) à Reducing bacteria (P. acne) on the skin à Reducing the inflammation à Exfoliation-peeling of the skin which unclogs pores

Policosanol is a mixture of fatty alcohols derived from waxy extract of sugarcane, containing a minimum of 55 – 60% Octacosanol

Anti-microbial Vitex

FOCUS HINTS

Vitex oil is extracted from the berries of Vitex agnuscastus and is standardized for 0.40% of Artemetin. It is made up of long chain fatty alcohols and long chain fatty acids like lauric, myristic, palmitic, stearic, oleic, linoleic and linolenic acids. The oil has potential anti-microbial activity. Vitex oil inhibits the growth of Propionibacterium acnes (anaerobic bacteria), E. coli (from 0.1%) and S. aureus (aerobic bacteria, from 0.5%).

à Staphylococcus aureus (a bacterial strain found in infected wounds and skin eruptions including acne) : Inhibition by 40% at 30µg/ml, so about 3x more than tea tree oil, and still 30% at 7,5 mg/ml when tea tree oil is then not active anymore à Staphylococcus epidermidis (a bacterial strain occurring in a variety of opportunistic bacterial skin infections): inhibition by ca. 30% at 30µg/ml, so double than tea tree oil, and still 15% at 7,5 mg/ml when there’s no more activity from tea tree oil. à Candida albicans : Inhibition by 40% at 30µg/ml and ca. 20% at 15µg/ml, so always more than Tea Tree Oil, without the unpleasant odor.

Sebum control Policosanol Policosanol is a mixture of fatty alcohols derived from waxy extract of sugarcane. It contains a minimum of 55 – 60% Octacosanol. Policosanol was found to have anti-microbial and sebum control effects that are potentially

Formulations may include as well the Cosmoperine - our Dermal Penetration Enhancer for a better efficacy, or Monolaurin , potent against P. acne

useful in managing inflammatory skin conditions, particularly acne. In a 2 week study on 16 human subjects between 8 and 25 yrs of age, 2% and 5% Policosanol colloidal solutions were found to be safe for local application. Topical applications of the Policosanol colloidal solutions were found to decrease the sebum secretion in a concentration dependent manner. (Reduction from 11.6% to 27.6%). Policosanol effectively compared with Clindamycin.

Cosmoperine Cosmoperine is a 95% tetrahydropiperine, prepared from pepper by a patented proprietary process. It may operate by increasing the membrane fluidity and affinity of drug / nutrient to the cell membrane, for a broad range of delivery through the skin. 12 - BECARRE Natural - October 2020

Cosmoperine has been subject to various studies : for instance, it has been found to enhance the Tetrahydrocurcuminoids (THC) due to increase penetration by min. 30% of the active across the barrier. Even at the highest dilution of 0.0001%. Other actives such as green tea polyphenols, syn-

Cosmoperine Tetrahydropiperine (THP) as enhancers of nutrient and drug bioavailability thetic pyrethroid, Albendazole, Betamethasone Dipropionate, etc... have been also performed with same prrofs of efficacy (absorbed faster rate and more completely) Recommended use : 0.01% to 0.1%

FOCUS

Cococin - Nourishment Factor® Cococin™, The Nourishment Factor¨ captures the goodness of green coconut water in a convenient powder form. Coconut water is the nutrientrich liquid endosperm of coconut abounding in proteins, amino acids, sugars, vitamins, minerals and growth hormones that support healthy cell growth & hydration. Coconut water solids are freeze-dried using a special process that preserves the activity of nutrients...

Moisturizer Promotes healthy cell / hair growth Supports blood circulation

Cosmetics and nutricosmetics Cococin is The Nourishment Factor® that provides a valuable nutrient pool for enhancing food and beverage, as well as cosmetic product formulations. Cococin can be safely used in oral care as well as lip care compositions. à Helps restore the skin health in aged and damaged skin à Augments new cell growth while promoting healthy tissues à Helps in wound healing à Helps in skin hydration

Cell Proliferation enhancement Cococin was used in the growth medium for fibroblast cell lines in an in vitro study (in Swiss 3T3 fibroblast cell line). Cell proliferation was significantly enhanced in cells treated with Cococin containing nutrient medium as compared to controls receiving standard nutrient medium.

Hair Growth Each individual hair is formed inside a hair bulb deep in a hair follicle. The follicle is a tiny but powerful factory, which throughout many people’s lifetime hardly ever stops working. From a baby’s birth for many decades, as much as a century in some people, the follicle continues to produce hairs. Finally the hair spontaneously falls out. The follicle rests for a little while, and then starts to produce yet another new hair.

Nutritionally versatile It is important to differentiate between coconut milk and coconut water. The ratio of RNA-phosphorus to DNA- phosphorus is significantly lower in the liquid endosperm of mature coconuts as compared to that of green coconuts. RNA plays an important role in amino acid transport and respiratory metabolism in living cells. Coconut water used to make Cococin is obtained from green coconuts at the optimal stage of maturity, to ensure a high content of RNA and growth factors, including shikimic acid, quinic acid and indole-3-acetic acid, along with essential vitamins, amino acids, and minerals.At the completion of growth, the solid endosperm and the last of the coconut water provide nourishment for the forming embryo and seedling. Thus, coconut water serves the role as a reservoir of nutrients to support tissue growth (Tulecke, et. al; 1961). Coconut water has been used in the tropics as a nutritive and rehydrating agent to restore electrolyte balance in cases of diarrhea (Adams, et al.; 1992). A published research report mentions that coconut water can be used as a short term intravenous (IV) fluid (CampbellFalck et al.; 2000). Other reported applications include use in total parenteral nutrition (TPN) (Petroianu, et al.; 2004), and sports beverages (FAO, 1998)0

Between starting to grow and falling out years later, each hair passes through three distinct stages : Anagen (the growing phase), Catagen (the intermediate phase), Telogen (the shedding phase). Cococin™ has been evaluated for its efficacy as a hair growth promoter, in comparison with Minoxidil as standard : Histological evaluation of the skin biopsies showed that the hair follicles were transformed from Telogen to Anagen phase of hair growth in the animals... showing a response to the topical application of formulations containing Cococin™ with hair growth promoting activity.

Cell Proliferation enhancement in Swiss 3T3 fibroblast cell line

Animals treated with reference standard viz 2% minoxidil showed telogen to anagen transition in more than 70% animals. The maximal induction of Anagen was observed with 2% Cococin™ cream ,which equaled that of the reference standard (2% minoxidil) in the experimental conditions of this study (see picture).

Reducing of skin aging

the

appearance

Twenty-two females in good health of ages 20-35 years old were subjects in a double blind placebo controlled clinical study (Research Report Sami Labs Ltd., 2005). A cream containing 1% coconut water solids (Cococin INCI: Cocos nucifera (coconut) fruit juice was applied onto the left arm of each subject, and the cream base was applied onto the corresponding area of the right arm, to serve as control. 200 mg of the cream and cream base was applied daily for eight weeks, to the marked areas on the left arm and right arm respectively (see figure)

Hair growth Histopathological (transversal) sections of animals

Hair growth Histopathological (longitudanal) sections of animals

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FOCUS

hile Rhodiola as a genus may have originated in the mountainous regions of Southwest China and the Himalayas, botanists have established that Rhodiola rosea naturally display a circumpolar distribution in mountainous regions in the higher latitudes and elevations of the Northern Hemisphere. In central and Northern Asia, the genus is distributed from the Altai Mountains across Mongolia into many parts of Siberia. Rhodiola rosea used for the production of RhodioLife® is wildcraft collected from this part, under the Russian Government License. Altai Mountains represents a pristine area free from contamination in of the most well preserved and remote natural environments. Nektium is involved from the early beginning in the collection practices, with SOPs describing the stage of the plant growth, best time of collection and ecologically non-destructive systems.

FOCUS HINTS

The root of R. rosea shows six distinct groups of chemical compounds. The Phenylalkanoids are the main contributors, incl. : • Phenylpropanoid : Rosavins are products of the phenylpropanoid metabolism. Rosavins (ie. rosavin, rosin and rosarin) are specific to the root from Rhodiola rosaea. The rosavins are the constituents currently most often selected as the marker compounds for standardization of extracts, although they are not necessarily the only pharmacologically active ingredients for its medicinal properties.

Rhodiola: Stress Support and Wrinkles – related illness. Rhodiola rosea has been demonstrated to be effective in different important applications:

Stress The effects of the stress on skin have been extensively proved. Stress is a well-known triggering factor in the appearance or exacerbation of psoriasis, alopecia areata, atopic dermatitis, acne, and other skin disorders. Panconesi et al. described the psychophysiology of stress in dermatology and found that the skin and central nervous system are related embryologically. The parasympathetic nervous system is critical to regulating our stress response. Rhodiola rosea enhances parasympathetic function and fortifies the stress response system, improving our ability to overcome stress and reducing our risk of stress-related health problems.

Anti-aging properties Application of Rhodiola rosea extract as a useful method of retarding the signs of photoaging and protecting the skin from UV damage. Photoaging can include signs of aging such us skin atrophy and means the thinning and/ or general degradation of the dermis caused by free radicals damage which is often characterized by an alteration and degradation of collagen and/or elastin due to extrinsic factors such as photodamage caused by exposure to UV radiation. Rhodiola rosea extract can help in the prevention or retarding skin aging, including wrinkles lines appearance, skin atrophy and thinning appearance reduction. Those properties of Rhodiola rosea extract are associated with: à DNA Repair à Collagen synthesis à Oxiden scavenger à UV Radiation absorption

• Principal phenylethanoids consist of glycosides and salidroside has been reported as the most active tyrosol glycoside, being associated, together with rosavins, to the anti-depressive and anxiolytic effects (Maslowa, 1994; Tolonen, 2003). Other phenylethanoids have also been isolated but with today limited scientific literature concerning the bioactivity (Jiménez 1994).

Figure on the right shows dose-dependent sunburn cell formation in living skin equivalents cells. Pretreatment with Rhodiola rosea significantly reduces the formation of sunburn cells via UVB irradiation. Rhodiola rosea pre-treatment significantly reduces UVB induced sunburn cell formation in LSE. Looking at the UV-Vis spectrum of Rosavins (Rosarin, Rosavin, Rosin), the absortion spectrum has a maximum in same wavelength than UV radiations.

Supplementing with Rhodiola rosea is a whole-body approach to maintaining health and wellness. While most supplements address specific conditions, Rhodiola rosea acts on many biological systems and can counteract one of the primary causes of illness – chronic stress, asthenia and premature aging. In today’s frenetic society, a one-size-fits-all supplement regimen with herbs and vitamins is ineffective. Each person is physiologically unique and requires a specific protocol for health. Supplementing with Rhodiola rosea is the foundation for an individualized supplement protocol because of its ability to heighten the body’s innate ability fight stress

Wrinkles

14 - BECARRE Natural - October 2020

Rhodiola rosea is also known historically as “Golden Root” from the “Golden Mountains” (Mongolian word ‘Altan’)

Enhances Skin Barriers : first, preventing your skin from wrinkling is protecting it from sun damage, which is the chief culprit in pre-mature aging of the skin. One study published in the June 2008 issue of the «Journal of Cosmetic Dermatology» showed that extracts of Rhodiola rosea improved the skin’s defensive barrier functions against the stress of UV rays. Subjects of this study were classified as having sensitive skin. Supports Elastin Production : Elastin is the rubber band effect in the skin that enables it to bounce back instead of sagging or wrin-

kling. Genetics play the largest role in determining how fast skin will wrinkle with the fairer skin having the highest risk of damage, according to the experts at SkinCarePhysicians.com. Applying Rhodiola rosea extract to delicate areas of the skin may help prevent premature wrinkling as the herb increases two types of molecular components needed by the body to create elastin. According to the cosmetic company, Origins, one of their skin care products containing Rhodiola showed significant reductions in line-wrinkles after an 8 week clinical test.

Supports Collagen Production The dermis is the second of three layers of skin, sitting just below the epidermis. The dermis consists of collective tissues, including elastin and collagen, which support the skin and provide its flexibility. When in good supply, elastin and collagen prevent sagging and wrinkling. Imagine the skin as a trampoline, which has to rebound after every stretch and strain. Rhodiola rosea elevates the number of hydroxyproline and hexosamine molecules, which are necessary for the body to make collagen.

Antioxidant and Stress Support Rhodiola rosea can be used to treat the body holistically as it contains neuro-supportive ingredients and antioxidants, which assist the body in coping with stress. Consider the effects of stress and the role it plays in the skin’s vibrancy. According to a February 17, 2010 article on the Fox News health blog, Rhodiola rosea contains pharmacological active compounds called rosavins, which are able to assist the body systems with oxygen deprivation, stress and immune support -- all important considerations for skin management.

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Forslean Coleus

Forslean® is manufactured by a proprietary process and is a standardized extract from the roots of the Coleus forskohlii plant, the only known plant source of forskohlin. ForsLean® is a registered trademark of Sabinsa Corporation.

Mechanism of Action The mechanism of action on how Forslean® works is well defined : «Forskohlin, the active compound in Forslean®, is recognized as an adenylate cyclase activator. Adenylate cyclase is the enzyme involved in the production of cyclic adenosine monophosphate (cAMP), a significant biochemical agent in metabolic processes. The role of cyclic AMP is indispensable to many body functions. It induces a chain reaction of biochemical events that trigger metabolic processes and diet induced thermogenesis, thereby providing the means to maintain healthy body composition and lean body mass levels.

Cosmeceutical applications Topical fat reduction

etraPure® is standardized for a minimum of 99% of Tetrahydrocurcumin (INCI Name: Tetrahydrodiferuloylmethane) : research reveals that Tetrahydrocurcumin (THC) is one of the major metabolites of Curcumin, reported to exhibit many of the same physiological and pharmacological actions as Curcumin, in some systems, better. Fungal infection of the skin is the 4th most common skin disease in 2014 Effecting more than 1.2 billion people around the world. A mycosis is a fungal infection of animals, including humans. Mycosis are common, and a variety of environmental and physiological conditions can contribute to the development of fungal diseases. Human mycoses may be broadly classified as : à Superficial, cutaneous and subcutaneous à Systemic

Dermatophytes It is observed that TetraPure® inhibits the growth of dermatophytes Trichophyton rubrum, Microsporum gypseum and Epidermophyton flocossum at all tested concentration (from 0.15%, Antifungal Efficacy – Agar Dilution Method).

in specific areas of the body is a common concern for women. Ronsard popularized the term “cellulite” to describe the dimpling and “orange peel” external appearance of the thighs, the cause of which was attributed to the aging process by later researchers. The structure of subcutaneous adipose tissue accounts for the development of the “orange peel” appearance. Groups of fat cells are attached to the

underside of the dermis by fibrous connective tissue. As fat cells enlarge, the fibers are stretched and pull down on the underlying skin. This causes the indentation or dimpling of the skin called cellulite. It has been demonstrated that adipose tissue metabolism varies from one region of the body to another, for example, in severely obese women losing weight after the jejunoileal bypass surgery, fat was seen to be absorbed more slowly in the thigh region than the abdominal region10. These differences lead to the hypothesis that localized application of agents that trigger lipolysis or fat breakdown could help in cases of fat accumulation at specific subcutaneous sites. Forskolin accelerates lipolysis through the activation of hormone-sensitive lipase — adrenoreceptors playing important roles in the regulation of lipolysis (adrenoreceptors are neurons that are activated by adrenaline (epinephrine) or similar substance). Based on clinical studies reported in literature, Coleus forskohlii extract 95% is potentially useful in dislodging localized fat deposits immediately under the skin, when applied topically. As an alternative to tanning by exposure to the sun that can cause skin cancer, topical application of forskolin induces tanning and is safer to use.

Dosage Form The recommended levels of use as a skin conditioning agent : 0.1 to 0.5% of a topical formulation, such as an ointment, cream or lotion.

Anti-fungal remedy for Skin, Hair & Nails Disease

Malassezia furfur Checking the activity against MF (Agar Dilution Method - Sabourauds Dextrose Agar with an overlay of coconut oil), it is observed that TetraPure® enhanced the activity at all tested concentration.

Candida Species It is observed that TetraPure® at 0.15%, reduces the colony counts of Candida albicans NCIM3471 (Yeast) from 14.6 105 to less than 100 cfu/ml with an overall percentage reduction of 99.99% over a test interval time of 28 days. Anti-fungal formulation : 0.15%w/w Skin lightening form: 0.1 - 0.5%w/w

Causative Organism

Incidence

Pityrisasis versicolor Seborrhoeic dermatitis including Dandruff and Follicular pityriasis»

Malassezia furfur

Tinea nigra

Exophiala werneckii

Rare

White piedra

Trichosporon beigelii

Common

Black piedra

Piedraia hortae

«Dermatophytosis Ringworm of the scalp, glabrous, skin and nails.»

Dermatophytes (Microsporum, Trichophyton, Epidermophyton)

Common

Candidiasis of skin, mucous membranes and nails

Candida albicans

Common

Dermatomycosis

Non-dermatophyte moulds Hendersonula toruloidea , Scytalidium hyalium, Scopulariopsis brevicaulis

Common

Rare

Tetrapure® inhibits superficial and cutaneous mycosis BECARRE Natural - October 2020 - 15