HPLC Application Note: 68-AB
Separation of Cephalosporins on HALO Penta-HILIC and HALO HILIC PEAK IDENTITIES: 1
1. 2. 3. 4. 5. 6. 7. 8. 9. 10.
G0050
Absorbance @ 254 nm
HALO Penta-HILIC 2 3 4
5
1
8
9
5
10
STRUCTUR G0051
HALO HILIC
1 2,3 4
0
6 7
6 8 7
9
10
2
3
4
5
6
7
8
9
Time (min.)
Cephalothin Cefoxitin Cefotaxime Cefazolin Cefaclor Cephalexin Cephradine Cefadroxil Ceftazidime Cephalosporin C
10
STRUCTURES:
TEST CONDITIONS: Columns: 2.1 x 150 mm Part Numbers: HALO Penta-HILIC: 92812-705 HALO HILIC: 92812-701 Mobile Phase: A= 95/5 ACN/H2O with 5 mM NH4formate, pH=3.0 (adj.); B= 50/50 ACN/H2O with 5 mM NH4formate, pH=3.0 (adj.) Gradient: HALO Penta-HILIC: 85-65% B in 10 min. HALO HILIC: 85-70% B in 10 min. Flow Rate: 0.5 mL/min. Pressure: 195 bar on HALO Penta-HILIC 163 bar on HALO HILIC Temperature: 30° C Detection: UV 254 nm, VWD Injection Volume: 0.5 μL Sample Solvent: 50/50 ACN/water Flow Cell: 5 μL semi-micro LC System: Agilent 1100
Cephalothin
Cefaclor
Cefadroxil
Cephalexin
Ceftazidime
Cephradine
Cephalosporin C
Cefoxitin
Cefotaxime
The HALO Penta-HILIC phase offers an alternate selectivity for the separation of cephalosporins in HILIC mode. Cefazolin
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