The Gram Stain
1. Add Crystal Violet for 1 minute
Rinse with Water
2. Add Iodine for 1 minute
Rinse with Water 3. Decolorize with alcohol solution until no color comes off (2-5 seconds)
Rinse with Water
4. Counterstain with safranin for 30 seconds
Rinse with Water 5. Air dry smear and observe under the microscope