Mycelium Project

Page 1

1.3 _ Growth

Organic

CIRCULAR ECONOMICS

OSTREATUS PLEUROTUS

and Shitak My e far celi um lab

r ate yw Gre lant P

04_ MYCELIUM

TEMPERATURE: RELATIVE HUMIDITY: DURATION: CO2: FRESH AIR EXCHANGES: LIGHT REQUIREMENTS:

THE THEORY OF CIRCULAR ECONOMICS ADVOCATES A CONSTANT RENEWAL OF WASTE MATERIAL THROUGH REUSE

BY STARTING AN URBAN PROPOSAL ON THIS BASIS, COMMODITIES, RESOURCES AND

al Loc

RECYCLING AND ADAPTATION.

and

ENERGY CAN BE APPROPRIATED IN A CYCLICAL TO REDUCE THE

WHILE SUBSTITUTING

PART SOLUTION, RECYCLING OFFERS LIMITED APPEAL AS ITS

od

etal

GOES BEYOND RECYCLING AS IT IS BASED AROUND A

Flo

THE CIRCULAR ECONOMY

TEMPERATURE: RELATIVE HUMIDITY: DURATION: CO2: FRESH AIR EXCHANGES: LIGHT REQUIREMENTS:

s

DEMAND FOR VIRGIN MATERIALS.

Hea vy M

DOWNGRADE MATERIALS, LEADING TO CONTINUING HIGH

70-80* F (21-27* C) 95-100% 10-20 DAYS 50,000 PPM OR 5% 0-1/HR N/A

THE POLYTHENE BAGS MAINTAIN THE 90% HUMIDITY REQUIRED FOR THE GROWTH OF MYCELIUM. THE BAGS CAN BE REMOVED FROM THE MOULDS FOR INSPECTION. AFTER THE GEOMETRY HAS

3a | Mould prep

BECOME MORE STERILE THE BAG CAN BE LOOSENED FROM THE SIDES TO ALLOW OXYGEN FILTRATE DEEPER AREAS OF THE

TEST MOULDS WILL CONTAIN ARE FORMATTED TO CONTAIN .008M^3 .027M^3 .064M^3 AND .512M^3 THEY ARE FORMED FROM 12MM MDF

SUBSTRATE.

PRIMORDIA (“ANTLER”) FORMATION:

farm

PROCESSES ARE ENERGY-INTENSIVE AND GENERALLY

w at er

wat er

SECONDARY MATERIALS FOR PRIMARY MATERIALS CAN OFFER A

al ion Nat

AMOUNT OF OVERALL WASTE.

7 a | Incubation

SPAWN RUN:

RESTORATIVE INDUSTRIAL SYSTEM GEARED TOWARDS DESIGNING OUT WASTE. THE DIAGRAM BELOW SHOWS A CIRCULAR ECONOMICS

65-75* F (18-24* C) 95-100% 14-28 DAYS 20,000-40,000 PPM 0-1/HR 4-8 HOURS AT 200-500 LUX

PROCESS BASED ON THE USE OF MYCELIUM AS A DECOMPOSER,

Existing industry

BIO-REMEDIATOR, FOOD SOURCE AND A BUILDING MATERIAL. THE SCALE OF THE NAVARRO SITE IS OF SUCH SIZE THAT A NEW RESOURCE PLAN IS REQUIRED FOR A SUSTAINABLE, RESOURCE AUTONOMOUS SOLUTION. THE CLOSED LOOP SYSTEM ALLOWS

Water

FLOWS OF ENERGY AND RESOURCES TO BE SHIFTED BETWEEN SCALES AND INDUSTRIES.

MYCELIUM ALLOWS FOR A SYMBIOTIC

6a | Inoculation Industrial

7 b | Incubation

A HERMETIC ENVIRONMENT IS MAINTAINED BY ENCASING THE SUBSTRATE IN A POLYTHENE BAG. THIS IS STERILISED USING ISOPROPANOL ALCOHOL PRIOR TO CONTACT WITH MYCELIUM

THE POLY-BAG IS STORED IN THE VERTICAL POSITION TO APPLY PRESSURE TO THE MATERIAL INSIDE. IT WAS FOUND THAT THE OBJECT DEFORMED AT THE LOWER PARTS RESULTING IN SPLITTING

LINK TO BE FORMED BETWEEN THE THRIVING AGRARIAN

AND TEARING OF THE BAG. IT WAS DECIDED TO SUSPEND THE BAG

INFRASTRUCTURE AND THE DEMAND FOR SOCIAL HOUSING AND RESOURCES.

FROM A HEIGHT TO REDUCE THE UNEVEN DISTRIBUTION OF

COFFEE BY PRODUCTS CAN BE USED AS A

MATERIAL.

NUTRIENT SOURCE FOR THE MUSHROOMS WHICH CAN THEN BE

CAUCA OR THE RIVER CALI.

House

Plastic Waste

g

stin

po

ly c om

Po

Organic Waste

TEMPERATURE: RELATIVE HUMIDITY: DURATION: CO2: FRESH AIR EXCHANGES: LIGHT REQUIREMENTS:

oam o_F Myc

Substrate

Industrial waste Agricultural waste Flood Water Grey Water Clean water

Fun·gus

INGREDIENT

SPAWN RUN:

Mycelium Usable mushroom

Waste

(fŭn-jī, fŭnghgī)

Individual

Unit

Cluster

Urban

MYCOLOGIST 75* F (24* C) 90-95% 12-16 DAYS 5000-20,000 PPM 1 PER HOUR N/A

FURNITURE STERILISED

AND ABILITY TO GROW AT AMBIENT ROOM

STRAW MYCELIUM

CLEAN

PLASTIC SHEETING

PRIMORDIA (“ANTLER”) FORMATION:

DUST FREE

CASTING MOULDS

TEMPERATURE: RELATIVE HUMIDITY: DURATION: CO2: FRESH AIR EXCHANGES: LIGHT REQUIREMENTS:

COMPARTMENT

50-60* F (10-15* C) 95-100% 4-5 DAYS 500-1000 PPM 4-8 PER HOUR 500-1000 LUX

TEMPERATURE.

MYCOLOGISTS

Room arrangement for hygiene

National

Myc-eilum (mī-sēlē-em)

Mush-room (’m |ru:m; -rųm)

1. (Botany) the vegetative body of

v1. (Botany) typically consisting of a cap (pileus) at the end of a stem arising from an underground mycelium 2. To Grow rapidly

throughout the nutrient substratum 2. 1. (Plants) any member of a kingdom of organisms (Fungi) that lack chlorophyll, leaves, true stems, and roots, reproduce by spores, and live as

THE SECOND COLUMN SAMPLE WAS AGITATED ONE WEEK INTO GROWTH TO DISTRIBUTE THE MYCELIUM GROWTH. AND ALLOW FOR AN EVEN DISTRIBUTION OF OXYGEN TO THE ORGANISM.

OYSTER SPAWN WAS OBTAINED FROM BRITISH SUPPLIER MUSHROOM. BOX. WE CHOOSE THIS SPECIES FOR IT’S TENACIOUS GROWTH

GANODERMA LUCIDUM

le tab ge e V

TO BE PUT BACK INTO THE RIVER

t lan

MYCOREMEDIATION CENTRES CAN BE SET UP TO DEAL

WITH ORGANIC AND WATER WASTE SO THAT IT DOES NOT HAVE

1 | Grain-spawn

rick

AND

FURTHER TO THIS LOCAL WASTE

p ste wa

NUTRITIOUS FRUITING BODIES.

My co_ B

TRANSFERRED TO A CALORIFIC OUTPUT IN THE FORM OF THE

[C19 (literally: nail of fungus): from myco- + Greek hēlos nail] mycelial adj myceloid adj

[C15: from Old French mousseron, from Late Latin mussiriō, of obscure origin]

and mushrooms

PROCESS

FOLLOWING THIS STAGE, ALL PARTICIPANTS MUST BE WEARING HAIR NETS, PLASTIC GLOVES UP TO THE ELBOW AND PAPER MOUTH MASKS AT ALL TIMES. ALL SURFACES TO BE WASHED WITH ISOPROPANOL ALCOHOL.

1 | Grain Spawn

2 | Raw Materials

A

C

B

D

Design thesis for Y Geometries 4 | Substrate Prep

5 | Pasteurisation

6 | Inoculation

7 | Incubation

2.1 _ Grow 1-3

f(x ) = x (2a+2b+c)

THE

5 | Pasteurisation THERE

THE GEOMETRY FOR THIS EXPERIMENT WAS TAKEN DIRECTLY FORM A PRE-EXISTING 3D MODEL. THE SURFACES WERE UN-ROLLED AND TABS ADDED TO ADJOINING EDGES. THE SHAPES WERE THEN HAND CUT OUT OF WHITE POLYSTYRENE PLASTIC.

60-80 DEGREES AND 1.5 HOURS. A LARGE SCALE

AUTOCLAVE IS THE MOST PREFERRED METHOD AS IT CAN BE EASILY MONITORED AND PROVIDES THE MOST CONSISTENT RESULTS.

IN

THIS

EXPERIMENT

WE

MAINTAINED

6c |Inoculation

HEATER.

4 | Substrate Prep

7 | Post Process

THE

2 | Raw Materials THE RAW MATERIALS OR SUBSTRATE FOR THIS PART OF THE PROCESS WILL FORM THE BULK, NUTRIENTS AND STRUCTURE FOR THE FINAL PROTOTYPE MATERIAL. THE ECOLOGICAL BENEFITS OF THIS MATERIAL COME FROM IT’S ABILITY TO UTILISE AGRICULTURAL WASTE MATERIAL. FOR THIS REASON WE HAVE LIMITED THE AREA IN WHICH WE CAN OBTAIN THESE MATERIALS TO A 100KM RADIUS AROUND THE SITE. IN THIS EXPERIMENT WE USED A 60/40 RATION OF STRAW AND OAK SAWDUST. BETWEEN 10% AND 20% WAS ADDED PER VOLUME

x

MYCELIAL GROWTH IS HAPPENS IN HIGH

THE PRODUCTION OF GRAIN SPAWN IS ACHIEVED THROUGH REPEATED

HUMIDITY, LOW LIGHT AND LOW AIR CHANGE

DUPLICATION OF A SMALL PIECE OF THE FRUITING BODY IN ANTIBIOTIC AGAR MEDIUM.

RHIZOMORPHIC MYCELIUM GROWTH IS CONTROLLED

UNTIL CELLS OF DESIRED STRENGTH CAN BE COLLECTED AND MIXED WITH ORGANIC MATERIAL WITHIN GLASS GRAIN SPAWN JARS.

SUBSTRATE MATERIAL WILL NEED TO BE PASTEURIZED PRIOR TO BEING PUT IN CONTACT WITH THE SPAWN GRAIN. SUBSTRATE AND WOOD-CHIP TO BE PLACED IN HEAVY DUTY CONTAINER WITH 60-80° WATER. PROCESS TO LAST BETWEEN 1-1.5 HOURS. CONTAINER TO BE HELD UNDER OBSERVATION FOR MAINTAINING STABLE TEMPERATURE.

ENVIRONMENTS.

FOR THIS REASON ALL MOULDS

WILL BE COVERED IN POLYTHENE SHEETS TO

FLAME-STERILISE THE SCALPEL UNIT GLOWING RED OVER THE ALCOHOL LAMP, LET COOL OFF AND PUT ON A STERILE BLADE. FOR

PROCESS OF MIXING SUBSTRATE IS TIME CONSUMING AND

LABOUR INTENSIVE IF NOT DONE MECHANICALLY.

THE

PROCESSES AS THE NUTRIENT RICH MATERIALS BECOME SUSCEPTIBLE

AS

MATERIALS ARE MIXED AND PREPPED FOR

PASTEURIZATION TIMING BECOMES CRUCIALLY IMPORTANT. PERIOD OF MORE THAN

IF

A

72 HOURS HAS PASSED BEFORE MIXING AND

PASTEURIZATION THERE IS THE POSSIBILITY OF THE FORMATION OF GREEN MOULD.

TISSUE HARVESTING WE RECOMMEND TO GRAFT TISSUE FROM THE ON

HYDRATING

AND MIXING OF THE SUBSTRATE SIGNIFIES THE START OF THE CLEAN TO CONTAMINATION.

THE

. SUBSTRATE PREPARATION IS NORMALLY DONE IN A

SEMI CLEAN ENVIRONMENT THAT IS SEPARATE FROM THE INOCULATION ROOM TO REDUCE AIRBORNE CROSS CONTAMINATION.

IN

PRIOR

TO INOCULATION A SHEET OF HEMP FABRIC

WAS REINFORCED WITH BAMBOO CANES. SUBSTRATE AND

MYCELIUM

A MIXTURE OF THE GENERIC

WAS LAYERED ON THE SHEET BEFORE

ROLLING THE ENTIRE OBJECT AND PLACING IT IN A THE PLASTIC TUBE.

ONCE

FITTED THE GAPS WERE RETROSPECTIVELY FILLED WITH THE

GENERIC SUBSTRATE AND

MYCELIUM MIX.

AERATED BAGS.

THIS

RESULTED IN A MUCH GREATER GROWTH AND

FOUND THAT DUE TO HIGH

THE

CO2 ATE PRE-FRUITING STEMS OF THE

HIS INNOVATIVE METHOD "PROVIDES A FUNGAL SUBSTRATE WHICH COULD BE MOLDED, AND EASILY AND CHEAPLY PREPROCESSED TO PRECISE GEOMETRIC SPECIFICATIONS," ACCORDING TO HIS PATENT APPLICATION . A SINGLE FUNGAL ORGANISM MIGHT COMPRISE MILES

USING BIOLOGICAL TECHNOLOGIES COMBINED WITH CUTTING-EDGE

MASS.

IRREGULAR GROWTH PATTERNS.

DURING HIS STAY, ROSS PRODUCED FOUR EASY CHAIRS --

ONE WHICH HE CAST IN A WHEELBARROW AS AN AESTHETIC TWIST AS WELL AS

THIS PROCESS ALSO FACILITATED THE 2 PIECES THAT WERE ALLOWED TO GROW TOGETHER.

DURING THE 3%HYDROGEN PEROXIDE SOLUTION IS ADDED TO REDUCE CONTAMINATION. THE BAG IS MIXING A

ALSO CLEANED WITH ALCOHOL FOR THE SAME REASON.

3|

2 | Raw Materials

T WAS

POSITIVE IS THEN FORMED IN PLASTIC USING HIGH

THE RAW MATERIALS OR SUBSTRATE FOR THIS PART OF THE PROCESS FORMED THE BULK, NUTRIENTS AND STRUCTURE FOR THE FINAL PROTOTYPE MATERIAL. A RATIO OF 4:1:1:.5 OAK SAWDUST, STRAW, GYPSUM AND OAT-BRAN WAS USED

50% WERE FRAIL AND FRAGILE. THE LARGER BLOCKS

RETAINED THEIR SHAPE BETTER AS THEY WERE DRIED IN PROXIMITY TO A RADIATOR.

Mould prep

FOR THIS EXPERIMENT A POSITIVE FOR THE MOULD IS CREATED BY LAYERING CNC MILLED MDF. THE IMPACT POLYSTYRENE A VACUUM FORMER.

WE

WERE

LIMITED IN GEOMETRY SIZE AS THE FORMER BED WAS ONLY

380X380MM

7 | Incubation THE

8 | Post Process

SAMPLES ARE ALLOWED TO GROW IN THEIR

2-3 WEEKS UNTIL A VISIBLE HAS FORMED ON THE TOP. THE

EACH OF THE SAMPLES WERE ALLOWED TO AIR DRY. IN

SEALED CONTAINERS FOR LAYER OF MYCELIUM

PREVIOUS EXPERIMENTS IT WAS FOUND THAT THE

PIECES ARE THEN REMOVED FORM THE MOULDS AND

RAPID

Grow closet

OF THE SUBSTRATE AND INCREASE GROWTH.

COMPUTATION AND ENGINEERING TO CREATE NEW BUILDING MATERIALS,

Substrate prep

Pre inoculation

Lab before inoculation

Inoculation process

Storage

SEALED CUPBOARD USED TO STORE MYCELIUM MOULDS, INTERNAL WALLS HAVE BEEN PARTIALLY INSULATED TO KEEP AMBIENT TEMPERATURE BETWEEN 7-12 C°

Sterile Table

THE LIVING USED A NEW METHOD OF BIO-DESIGN, 100% ORGANIC MATERIAL.

RESULTING IN A STRUCTURE THAT IS

A TABLE ONLY USED WHEN WORKING WITH THE MYCELIUM DIRECTLY

THE STRUCTURE TEMPORARILY DIVERTS THE NATURAL CARBON CYCLE

Ante-room

TO PRODUCE A BUILDING THAT GROWS OUT OF NOTHING BUT

- WITH ALMOST NO THIS APPROACH OFFERS A NEW VISION FOR SOCIETY'S APPROACH TO PHYSICAL OBJECTS AND THE BUILT ENVIRONMENT. IT ALSO OFFERS A NEW DEFINITION OF LOCAL MATERIALS, AND A DIRECT RELATIONSHIP TO NEW YORK STATE AGRICULTURE AND INNOVATION CULTURE

SEALED ENTRANCE ROOM WITH DOUBLE SKIN OF PLASTIC SHEETING TO REDUCE DRAFTS OF

WASTE, NO ENERGY NEEDS, AND NO CARBON EMISSIONS.

CONTAMINATED AIR AND KEEP AMBIENT ROOM TEMPERATURE HIGH

Aluminium pots

COMMERCIAL KITCHEN POTS USED TO PASTEURISE STRAW AND TRANSPORT WATER

Medium Sterile table

POLYCARBONATE LAYERED WORK TABLE USED FOR NON MYCELIUM BASED TASKS AND

--

20 SMALL STOOLS ($300 EACH) AND SIX CYLINDRICAL ($350 EACH).

GENERAL STORAGE OF CLEANED PRODUCE

Substrate drainage table

SMALL BENCH WITH PVC CHANNEL USED TO FOR DRAINING AND DRYING STRAW.

Sterile storage

A DISCONNECTED FRIDGE WHICH HAS BEEN USED TO STORE CLEANED MATERIALS AS WELL AS

The lab during experiment 1

Diagram of lab showing hierarchy of surface

BEN SHETERLINE PRICE + MYERS BEN JOINED PRICE & MYERS AFTER GRADUATING FROM UNIVERSITY OF WARWICK IN 2006. HE HAS WORKED ON A WIDE VARIETY OF REFURBISHMENT AND NEW BUILD PROJECTS. PRICE MYERS ARE A LEADING LONDON BASED ENGINEERING FIRM THAT ALSO SPECIALISE IN SUSTAINABILITY REPORTS AND ASSESSMENT. THE COMPANY HAS BEEN VERY FAVOURABLE TO OUR MYCELIUM AND EPS WORK AND HAS SHOWN A KEEN INTEREST IN OUR INNOVATIVE APPROACHES TO CONSTRUCTION

PHIL ROSS MYCOWORKS

WE MET DEBORAH AT THE BRE DRAGONS DEN SEMINAR

AFTER INVITING EBEN TO VISIT OUR LAB ON A VISIT TO LONDON HE OFFERED TO PARTNER WITH US BY SUPPLYING US

OF THEIR CONSTRUCTION INNOVATORS PROGRAMME.

DR DEBORAH PULLEN IS THE GROUP RESEARCH DIRECTOR AT THE BUILDING RESEARCH ESTABLISHMENT (BRE), LEADING THE DELIVERY OF THE CURRENT £20M RESEARCH AND PUBLICATIONS WORK BOTH AND AT BRE AND ITS FIVE UNIVERSITY CENTRES OF EXCELLENCE. THIS ALSO ENCOMPASSES THE BRE TRUST FUTURE CITIES PROGRAMME, WHICH NOW HAS OVER 70 PARTNERS FROM ACADEMIA,

THEM IN NEW FORMATIONS TO MAKE THEM STRONGER AND MORE VERSATILE AND THEN USING THEM AS A MATERIAL WITH

HIS WORK WAS HIGHLY INFLUENTIAL TO THE

PROGRESS OF OUR RESEARCH AND HE HAS BEEN A VALUABLE CONTRIBUTOR

EBEN BAYER ECOVATIVE

WHEN WE WERE PRESENTING OUR MYCELIUM WORK AS A PART

PHIL ROSS ACTED AS MENTOR FOR THE MID TO EARLY STAGES OF OUR MYCELIUM RESEARCH . HE IS A SPECIALIST FOR ALMOST 20 YEARS HE HAS BEEN EXPERIMENTING WITH MUSHROOMS FOR THEIR RECYCLING PROPERTIES, GROWING

WHICH TO BUILD.

DR. DEBORAH PULLEN MBE B.R.E

IN APRIL WE PRESENTED OUR WORK TO A BRE RESEARCH PANEL IN WATFORD.

A HIGH QUALITY MYCELIUM PRODUCT AT A HEAVILY SUBSIDISED RATE.

BAYER CO-FOUNDED ECOVATIVE IN 2010 WITH HIS GAVIN MCINTYRE, WHOM HE MET WHILE THEY

COLLEAGUE

WERE BOTH STUDYING MECHANICAL ENGINEERING AT

RENSSELAER POLYTECHNIC INSTITUTE IN TROY, N.Y. SINCE THEN ECOVATIVE HAS RAISED OVER $14 MILLION IN FUNDING, AND ITS 65 EMPLOYEES ALREADY PRODUCE PACKAGING FOR COMPANIES LIKE DELL AND STEELCASE. THE PROCESS

INDUSTRY AND PUBLIC SECTOR WITH A COLLECTIVE VALUE OF

INVOLVES USING AGRICULTURAL WASTE TO GROW

£10M. WE ARE CURRENTLY EVALUATING THE POSSIBILITY OF

MUSHROOMS—AND, MORE IMPORTANT, THEIR BINDING

BUILDING THE PROTOTYPE AT THEIR FACILITIES PRIOR TO SHIPPING TO

COLOMBIA

VEGETATIVE ROOTS KNOWN AS MYCELIUM—IN MOLD FOR TO

120 HOURS

96

SUSANNA HORN JARDEMARK. + ANITA OLLÁR DURING OUR SECOND GROWTH EXPERIMENT IN MARCH SUSANNA AND ANITA JOINED FROM CHALMERS UNIVERSITY SWEDEN. THEIR WORK IS PREDOMINANTLY CONCERNED WITH MYCELIUM GROWTH AND VARIETIES IN MEDIA.

LAURA AND OUR MYCELIUM BLOCK ON THE FRONT OF

CONSTRUCTION MANAGER MAGAZINE.

DEHYDRATION

OF

MATERIAL

CAN

CAUSE

UNWANTED SHRINKAGE AND DEFORMATION.

PLACED IN A LARGER BAG TO FACILITATE THE AERATION

3.1 _ The LAB

BASKET-LIKE PIECES

PARTNERS + EVENTS

8 a| Post Process IN THIS EXPERIMENT WERE ALLOWED TO

I

PLACED IN A SEALED POLYTHENE BAG.

PASTEURISATION

FOUND THAT PIECES WITH A VISIBLE INOCULATION OF LESS THAN

HOSTS INDEPENDENT BUILDERS AND DESIGNERS FOR TWO MONTH STAYS.

BOXES

DRY NATURALLY USING NATURAL VENTILATION.

MUSHROOMS WERE DEFORMED CAUSING LENGTHENING OF STEM AND

EARTH AND RETURNS TO NOTHING BUT EARTH

THE LATEST CREATIONS TO EMERGE FROM ROSS'S MUSHROOM MOLD ARE EASY CHAIRS, WHICH HE SELLS FOR $3,000 APIECE. HE BUILT THEM DURING AN EIGHT-WEEK RESIDENCY AT THE WORKSHOP, AN INDUSTRIAL WORK-SPACE IN SAN FRANCISCO THAT

TEST

G. LUCIDUM. MOULDS ARE THEN

EITHER SEALED WITH A POLYTHENE SHEET OF ELSE

A SUBSTRATE WITH RATIO 4:1:.5:.5 OF OAK SAWDUST, STRAW, CALCIUM CARBONATE AND OAT-BRAN ARE MIXED BY HAND AND HYDRATED. THE MIXTURE IS THEN PLACED IN NYLON ROASTING BAGS IN PREPARATION FOR

CHARRING OR DEGRADATION

THE FORMATION OF A THICK SKIN AROUND THE EXTERIOR. IT WAS

GRAIN SPAWN OF

WHICH SMALL INCISIONS WILL BE MADE TO

2 | Hy-Fi tower

ROSS CONCENTRATES THEM INTO A DENSE, LOAD-BEARING

THE RESULT WAS A DENSE AND

MONOLITHIC FORMATION WITH LITTLE TO NO SIGNS OF

THE INOCULATION PROCESS SOME OF THE MOULDS WERE

ADEQUATE TIME TO COOL THEY ARE MIXED WITH THE

4 | Substrate Prep

CONDENSED COLUMN GROWN IN A POLY TUBE WAS

REMOVED FROM THE PLASTIC BAGS AND PLACED IN LARGER MORE

THIS EXPERIMENT THE FORM-WORK WAS A SIMPLE TUBULAR

POLYTHENE BAG.

7 c| Incubation DURING

3+6b | Agar Culture

8b | Post Process

6a | Inoculation AFTER THE SUBSTRATE BAGS HAVE BEEN GIVEN

ALLOW ADEQUATE OXYGENATION.

1 | Mycoworks

FOREST;

COMBUSTION IN AREAS WITHOUT MYCELIUM GROWTH.

KILN DRIED FOR ONE HOUR.

5 | Pasteurisation THE PLASTIC BAGS ARE PLACED IN A POTTERY KILN FOR 3 HOURS AT 100 DEGREES TO KILL ANY COMPETING SPORES OR CONTAMINATING GERMS. THE INTERNAL TEMPERATURE OF THE BAGS IS MAINTAINED AT A TEMPERATURE BETWEEN 60-80 TO STOP THE DEGRADATION OF CELLS.

SAMPLES PLACED IN THE KILN WERE DAMAGED DUE TO

THE

LOCATION PRECEDENT OF FIBROUS STRANDS FANNING BENEATH THE SURFACE OF THE

THIS IS DUE TO THE THINNER ELEMENT SIZE AND THE INCONSISTENT GROWTH.

1.2 _ Grow 4-6

THIS

ENVIRONMENT THROUGH A DOMESTIC COOKING POT AND RING

3 | Mould Prep

HANDLED.

3c | Mould Prep

ARE A NUMBER OF METHODS THAT CAN BE USED TO

HEATED TO A TEMPERATURE BETWEEN

f(x ) = x (2a+3b+4d)

SAMPLES ALLOWED TO DRY NATURALLY WERE VERY

DELICATE AND IN MANY INSTANCES WOULD CRUMBLE IF

PASTEURIZE. IN EACH OF THESE INSTANCES THE SUBSTRATE IS KEPT AT THIS TEMPERATURE FOR

8c | Post Process

Climatisation

JUNE

RESEARCH

WEEK 4

WEEK 3

WEEK 2

MAY WEEK 4 WEEK 3

WEEK 2

APRIL

WEEK 4

WEEK 3

WEEK 2

MARCH

WEEK 4 WEEK 3

WEEK 2

FEBRUARY

JANUARY

WEEK 4

WEEK 3

WEEK 2 DECEMBER

NOVEMBER

OCTOBER

SEPTEMBER

EXPERIMENT


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