GLYCOMICS PROFILING GLYCOMICS ANALYSIS BY MASS SPECTROMETRY introduction of glycans Glycomics is the study of glycans that focuses on the the structure and func�on of carbohydrates. Glycoform distribu�ons at the cellular, �ssue, organ and organism levels are also studied. Three aspects of glycomics are usually explored, including structural characteriza�on of glycans, research of glycan-protein interac�ons, and the study of in vitro and in vivo systems for the determina�on of the func�on of specific glycans.
The majority of the linear sugars are glycosaminoglycans (GAGs) that consist of repea�ng disaccharide units. The units are 0-linked to a core protein, forming a proteoglycan aggregate.
Linear
Branched glycans are present as N-linked and O-linked glycosyla�on on glycoproteins or on glycolipids.
α3/6 α3/6 α8
α4
α2 α3 α3/β4
α4
α3 β4
α3
β3 α2
α2
α2
α2 α6
α3
β4
β4 β2
α6
α3
β2
β4/6 β4
α3
α3
α6
β2
β4/6
β4
β4
β4
β4
β4
β4
β4
β4/6
α6 α3
β3 β3
β4 β3
α3
β6
β3
β3
β3
β4
β3
β6
α6
Asn
Asn
Hybrid
Complex
Asn
High mannose
Branched
Ser/Thr Ser/Thr
β3
α3
α4
β4
β4
β4
β3
α3
α4
β4
β4
β3
β3
β3
β3
β3
β3
β4
β4
β4
Ser/Thr Ser/Thr Ser/Thr
Ser/Thr Ser/Thr
Core 1 Core 2 Core 3 Core 4
N-linked glycans
β4
HS
O-linked glycans
CS
DS
Glycosaminoglycans ( Figure reference to Hyun J Y. et al., 2017)
Proteoglycans glycosaminoglycan
Ser/Thr
Glycoproteins
Glycosphingolipids
Ser/Thr
O-glycan
Glc Man
GalNAc Xylose
Gal NeuNAc
GlcNAc Fuc
N-glycan
Asn
general glycomics strategies
Ser Thr
Ser Thr
Asn
Asn
Diges�on
Ser Thr
Glycan
Asn
Asn
Body Fluids
Release
Asn
Ser Thr
Asn
Cells/Tissues
Glycolipids
Release
Glycan Release
Glycans
Glycosaminoglycans
Free Glycans
Deriva�za�on
HPLC Separa�on
Mass Spectrometry
b2
b3
Intensity
y2
Proteoglycans
Glycan
Glycopep�des
y3
Glycoproteins
m/z
services and advantages
N-Glycan / O-Glycan Profiling N-Glycosyla�on / O-Glycosyla�on Site Occupa�on Analysis N-Glycan / O-Glycan Linkage Analysis Structural Characteriza�on of Glycans
Technology Pla�orm: MALDI-TOF MS UPLC-FLD/MS HPAEC-PAD Microarray NMR spectroscopy
Glycopep�des Analysis
High Sensi�vity and Specificity
Glycans Microarray Assay
Stability and Consistency
Poysaccharide Analysis
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Pep�doglycan Structure Analysis
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Glycan Microarray