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Datasheet of Cl-amidine
Description: Cl-amidine is a cell-permeable compound that acts as a pan PAD inhibitor (IC50 = 0.8 μM, 6.2 μM and 5.9 μM for PAD1, PAD3, and PAD4, respectively) in enzymatic assays. It preferentially inactivates the calcium bound form of PAD4 in an irreversible manner, and is shown to complete with BAEE for the active site residue of this enzyme. A small but significant reduction in the efficiency of p300GDB-GRIP1 interaction is observed in transiently transfected CV-1 cells treated with Cl-amidine at concentrations between 25 μM and 200 μM, in vivo . Furthermore, this compound (200 μM) together with HDAC inhibitor SAHA (0.4 μM) has shown additive effects in inducing p21, GADD45, and PUMA expression and inhibiting cancer cell growth in a p53-dependent manner in U2OS cells. \Chemical
Information
Catalog
DC9725
Purity of current batch:
>98%
Molecular Weight (MW)
424.8
Molecular Formula
C16H20ClF3N4O4
CAS No.
1043444-18-3
Solubility (25°C)
DMSO
Storage
Store at -20°C (desiccating conditions).
Handling: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months. Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.