9/17/2019
Biochemistry Laboratory Techniques - Edukite
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Biochemistry Laboratory Techniques ( 9 REVIEWS )
477 STUDENTS
The course focuses on the research-inspired laboratory experience in biochemistry eld. You will be introduced to the standard biochemical techniques …
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LEVEL 2 - CERTIFICATE COURSE BADGE COURSE CERTIFICATE NUMBER OF UNITS
8
NUMBER OF QUIZZES
0
3 HOURS, 20 MINUTES
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The course focuses on the research-inspired laboratory experience in biochemistry eld. You will be introduced to the standard biochemical techniques so that you can able to investigate the present and existing research topics. Particular focus will be given on the chemical techniques of the cancer drug Gleevec. Apart from these, the course focuses on the protein expression, puri cation, and gel analysis, PCR, site-directed mutagenesis, kinase activity assays, and protein structure viewing.
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COURSE CURRICULUM
Lec 1 Introduction. Kinases in healthy and cancerous cells
00:30:00
Lec 2 DNA digestion and PCR
00:35:00
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Biochemistry Laboratory Techniques - Edukite
Lec 3 Af nity tags for protein puri cationdetection
00:40:00
Lec 4 Conserved and variable structural features of kinase domains
00:45:00
Lec 5 Site directed mutagenesis (Quikchange method)
00:20:00
Lec 6 Detecting kinase activity
00:30:00
Assessment
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