CEVA - Vet Services Bricks 2023 by agriNews

Bricks from 2022

From green brick of Knowledge

to Fortress of Veterinary Excellence Consider each piece of veterinary information as a brick. Alone, it’s simple. Yet, when combined, these bricks form a resilient wall, building up to a fortress of veterinary expertise. This booklet embodies these bricks, with its four crucial chapters: 1 GPS REPORTS - Monitoring and awareness insights. 2 Serological Baselines and ELISA kits - Precision in diagnostics. 3 Layers and Breeders - Addressing Salmonella, FadV, IBH, and beyond. 4 Field Support and Troubleshooting - Hands-on help and solutions in real-time scenarios.

Introduction. From green brick of Knowledge to Fortress of Veterinary Excellence

Across the world, you as vet services managers face diverse challenges, but all tread the path of rigorous fieldwork. Through dedication, discipline, and tools like this booklet, you’re forging a path towards ‘vet services excellence’.

As Winston Churchill once said,

Success is not final, failure is not fatal: It is the courage to continue that counts.

Let this booklet be the North Star that guides us on our journey to veterinary excellence.

Chapter 1.

GPS reports, awareness & monitoring GPS is at the heart of your core activities, as you all know. That’s why we will start with a brick where we highlight the importance of sharing and presenting appropriate reports in pulse meetings with your customers. Awareness, monitoring and troubleshooting are the three main activities which shall be included in your reports to create the highest impact in your conversations.

Chapter 1. GPS reports, awareness & monitoring

Brick No. 2 “GPS reports in the PHP”

During 2020-2022, the Veterinary Services Managers (or other colleagues) uploaded 1033 reports in GPS-PHP. See in the table below the split per zone:

AWARENESS

MONITORING

TROUBLESHOOTING

TOTAL

ASIA

342

171

595

EEMEA

170

351

WEU

LATAM

NAPAC

TOTAL

203

547

283

1033

The quality of the reports regarding the content and format is very good. The information contained could bring a lot of value in the discussion with your customers and it helps to follow up the field activities of the Ceva teams. We have chosen 3 different reports (one for awareness from Thailand, one for monitoring from South Africa and one for troubleshooting from Intertropical Africa) demonstrating all your skills.

REPORT

T CARGILL MEA THAILAND

VISIT REPORT MALINDO Company name: Sardju Farm Name: r: Seno General Manage Hartono Customer Vet: ignatia.tiksa@ceva.com by: Visit created t-12 Visit Date: 2021-Oc : 2022-Mar-08 edition Report date

UL Natthika TORSAK e Vet Service Associat

27/1/2022

Example of a monitoring report in a South African poultry producer.

Example of an awareness report in a strategic account in Thailand.

Example of a troubleshooting report in a key account in Intertropical Africa.

Brick No. 4 “Vectormune ND awareness and monitoring” See below a compilation of all the information presented in the Global Veterinary Services about Vectormune® ND. The webinars are good opportunities to review our strategy and to update the knowledge about specific key topics important for all of us. For example, here we highlighted the importance of awareness, depending on the challenge situation of the country, setting the value for Vectormune® ND implementation. Once the lesson is reviewed, you usually need to complete a quiz to fix the concepts.

Chapter 1. GPS reports, awareness & monitoring

Brick No. 7 “IB Awareness: Asia Example” This brick is a good occasion to insist on awareness by describing two IB awareness experiences in Asia (Thailand and India), summarizing in one dashboard all the work done for the projects, reminding the “modus operandi” and displaying 2 methods to deliver back the results to the customer:

1 Serological interpretation to select the right flocks (Thailand).

2 Correlation between serology & performance (India).

IB Awareness process Serology + PCR added to Performance results oriented (FCR, condemnation (SRC)/focus analysis) Samples

SERA

Technique

10 sea per house or 30 sea per farm Individual Serological Titers to compare with vaccination program standard

Broiler sampling protocols (farm/ slaughterhouse) Trachea/ caeca tonsils

10 trachea per house or 30 trachea per farm + 10 caeca tonsils per house or 30 caeca tonsils per farm Vaccine take PCR: Demostrate that the vaccine take is irregular General PCR sequencing: Demostrate that field IBV strains are present

Expected results 100% individual serological results compatible with at vaccination program level Some very low or some very high titers; High FCR; Aerosacculitis; respiratory symptoms in farm; high mortality in truck

Many negatives (other diseases) and indications of Ibv field infections

Chapter 1. GPS reports, awareness & monitoring

Brick No. 16 “Gumboro Awareness and nvIBDV reassortant strains” Awareness is not only important for respiratory diseases, but also, for example, for Gumboro disease. Indeed, one of the key main outputs of our Global Disease Awareness Plans (2020-2022) were shifts in the IBDV populations, confirming the emergence of new variants. The key points observed were:

1 A new variant of the virus (nvIBDV), phenotypically different and genetically reassortant, was detected, confirming the previous works of Phylaxia (Mató et al., 2020).

2 Investigations at farm level (PhD) to describe the symptoms and lesions are ongoing in collaboration with MAPS Padova, University Complutense of Madrid and Phylaxia.

3 The results will be accompanied by characterization, challenge investigations and performance analysis.

Chapter 1. GPS reports, awareness & monitoring

Brick No. 17 “Q&A’s about nvIBDV reassortants” The bricks are good opportunity to answer many questions raised by you. In the last brick (number 16), new variant reassortant Gumboro strains were described, therefore, we have tried to address these questions in this brick.

Two extra slides with the micro- and macroscopical characteristics of the nvIBDV reassortant strains have been included (from Mato et al., 2020)

Chapter 1. GPS reports, awareness & monitoring

Chapter 2.

Serological baselines and ELISA kits These are your guidelines to understand and differentiate the effect of several vaccination programs, showing the expected seroconversion results and the kit to be used based on the implemented vaccination program (innovative, conventional or mix) and how to differentiate vaccination from field challenges.

Chapter 2. Serological baselines and ELISA kits

Brick No. 1 “Newcastle serology baseline” Based on the vaccination program implemented, you can select the ELISA kit to be used and the expected titers you will get at slaughter age. A significant differentiation is also available between Vectormune® ND and other recombinant products. This brick also defines the max. expected titers, where we can clearly identify a field challenge.

Chapter 2. Serological baselines and ELISA kits

Brick No. 3 “Gumboro serology baseline” This slide provides you with the expected seroconversion titers for different Gumboro IBD vaccination programs and how to differentiate immunity provided by the vaccine from field challenges.

Brick No. 5 “Monitoring of IBIRD/IBRON. Vaccine take and serological results” This brick aims to provide a tool for monitoring vaccine take and for serology interpretation, understanding the IBV flock situation and challenges. This is a key factor of success for the implementation of our bronchitis vaccines.

Chapter 2. Serological baselines and ELISA kits

Brick No. 6 “ILT and Vectormune® FP-LT/ vaccination quality and monitoring” ILT remains a major threat in poultry production. Most of the time live vaccination leads to outbreaks through reverse virulency of the C.E.O/T.C.O vaccine itself. Vectormune® FP-LT is the safest vaccine providing birds with the needed immunity to control the disease without inhibiting the control of other diseases with the innovative recombinant HVT vaccines. This brick aims to provide a tool on the specific kit to detect the ILT seroconversion provided by Vectormune® FP-LT from field challenge and highlight on ILT serological baseline to assess the flock situation.

Chapter 2. Serological baselines and ELISA kits

Brick No. 10 “Follow up on Newflend® Vaccination” Through this brick we are trying to summarize in a slide several steps in Newflend® vaccination follow-up.

1 Detailed clarification of sampling age. 2 Tool for you to interpret the results. 3 Detect the “Mutual Conspiracy” effect. 4 5

Perform the DIVA test to differentiate between Immunity and field challenges. Prove that Newflend® is providing local, cellular and humoral immunity that will help birds to withstand challenges and perform better yield.

® is replicating as fast 6 Prove that Newflend ®

as Vectormune ND and providing the same high level of protection, especially on ND.

Brick No. 12 “ELISA kit selection per disease” Sometimes it is a challenge to choose the kit to monitor a vaccination program. This brick is serving as a guideline to choose the right kit, according to field challenge situation and vaccination program.

Chapter 2. Serological baselines and ELISA kits

Chapter 3.

Layers & Breeders: Novamune, Salmonella, FADV IBH & IBH vaccine, molting Here we are gathering specific and very useful information that will help you to better understand specific long-living birds’ pathologies and the key features of our innovative vaccines.

Chapter 3. Layers & Breeders: Novamune, Salmonella, FADV IBH & IBH vaccine, molting

Brick No. 23 “Cevac IBH-8K” The presence of certain Adenoviruses can compromise the immune response and reduce vaccine efficacy. Ceva is very responsive to customer concerns and therefore we have developed for Argentina an inactivated vaccine including serotype 8b with great results in the field in breeders.

Chapter 3. Layers & Breeders: Novamune, Salmonella, FADV IBH & IBH vaccine, molting

Brick No. 19 “Immunization and molting in layers” Bird molting is sometimes a common practice in commercial laying birds. Herpesvirus replication is boosted during stress phases (like molting). rHVT Ceva vaccines would fit perfectly for this, especially in immunosuppressed birds that undergo molting, representing this a competitive advantage versus live and inactivated vaccines.

Chapter 3. Layers & Breeders: Novamune, Salmonella, FADV IBH & IBH vaccine, molting

Brick No. 15 “Adenovirus infection” Guidelines to investigate Fowl Adenovirus Inclusion body hepatitis (FAdV-IBH).

1 The presence of IBH can compromise

the efficacy of vaccination, specially Transmune®, Nextmune® or Novamune®.

2 Highlighting FAdV-IBH diagnosis to protect

broiler flocks and to allow you to address these issues professionally in front of your customers.

Brick No. 14 “Cevac Salmovac® monitoring” Salmovac® monitoring is essential to ensure a proper application of the vaccine and subsequent immunization of the birds. It is an advantage over our competitors and must be performed, at least, after the first application.

SALMOVAC

Chapter 3. Layers & Breeders: Novamune, Salmonella, FADV IBH & IBH vaccine, molting

Brick No. 11 “Salmovac® in Broiler breeders” Cevac Salmovac® is an excellent vaccine capable of controlling Salmonella outbreaks in the field, being a key tool to control Salmonella in broiler breeders. Here you will find the key steps to ensure correct vaccination in broiler breeders.

Chapter 3. Layers & Breeders: Novamune, Salmonella, FADV IBH & IBH vaccine, molting

Brick No. 8 “Novamune®” The expected colonization of the bursa of Fabricius in layers vaccinated with Novamune® is compared to colonization by foreign vvIBDV strains in birds vaccinated with rHVT-IBD. Layers vaccinated with recombinant vaccines are not able to block the Gumboro cycle.

Chapter 4.

Field support: early health in chicks, short sampling guidelines, biosecurity, vet program reporting, troubleshooting tools This chapter explains how to manage field visits in regular and critical situations such disease outbreaks. Ceva has developed innovative tools to boost the impact of your farm visits and to support you in your daily activities.

Chapter 4. Field support: early health in chicks, ssg, biosecurity, vet program reporting, troubleshooting tools

Brick No. 9 “Short sampling guidelines” During the farm visit, necropsy is a key time and the vet services manager need to choose the appropriate samples (organs, support, analysis asked to the lab) to expect the best analysis results. This short sampling guidelines help you to not forget something in the sampling protocol.

SHORT SAMPLING GUIDELINE

DATION

bs >

DOCUMENTS

SAMPLING TOOLS

• Special import permit is required for shipment to a foreign lab

Troubleshooting investigation sheet

Head lighter

Samples history sheet

Flam torch gun

• The packaging must have three components

Sampling guide

Indelible marker Blood sample tube

BIOSECURITY TOOLS

• Refere to sample shipment SOPs • Avoid the week end and days off • Hermetically sealed bottles

Disposable coverall

Blood tubes holder

Plastic boot bag

Sterile container (organs sample) 30 ml

Disposable charlotte headdress

10 empty Containers 100 ml solution Bottle (histology)

Mask

Plastic bottle with disinfectant for the used disposable (blade, needles...)

Disinfectant bottle spray

• organs volume = 1/3 of the total volume

Disposable latex gloves

1l with 10 % formalin solution (hermetically sealed bottle)

Trash bags

Plastic zipper bag

SAMPLING TOOLS

• Only for a local lab

Necropsy tools • Special packaging is required if shipment to foreign lab

Forceps

Ice box

Blade

Dacron swab with plastic handle

Scalpel handle

Ice pad

Utility scissor

FTA card

LAB ADRESSES TEST

• Normal shipment by DHL in an ordinary envelop

LABORATORY

ADDRESS

Culture

• Fill accurately the SHS on susy platform

BACTERIOLOGY

Sensitivity

• Should be shiped frozen with carbonic ice/ice pad

• Normal shipment by DHL in an ordinary envelop.

Photos : Ceva / ??????????????????????????????????????????????????

EQUIPMENTS: FARM VISIT NEEDS

SHIPMENT CONDITIONS

PCR & SEQUENCING

Elisa

SEROLOGY

Rapid Plate Agglutination

HISTOLOGY

SAMPLES HANDLING RECOMMENDATIONS

Frozen

Formaline 10%

Swabs

Dry

STORAGE CONDITION

SPECIAL RECOMMENDATION SAMPLING

• If test < 12h => T° at 4°C

• If test > 12h => frozen at -20°C or swabs

• In 100 ml container with formaline 10%

• If samples received in the laboratory before 24hours from the sampling time (4°C)

• Entire organs should be sampled zipper individual bag/organ ideally (1 birds/bag is accepted)

• Only organs sampled from euthanized birds • All organs belonging to one bird in the same bottle except if only one organ type is targeted • 3 cm3 organ pieces max (1 cm3 is optimum) • Well disinfect the organ surface with gun fire

Wet

• Up to 7 days at 4°C (ideal <4 days)

RNA virus

• Storage 4-8°C

DNA virus

• Room T° or 4-8°C

• Sampling identification on the FTA before the sample (circle & card) : flocks & organ Identification

• Storage 4-8°C

SHIPMENT CONDITIONS

• For analysis to be done in the local lab

• Special import permit is required for shipment to a foreign lab

• The packaging must have three components

• For analysis to be done in the foreign lab

• Refere to sample shipment SOPs

• Crush the swab on the FTA card to extract the liquid containing the mycoplasma

• Maximum 6 months

Serum

Blood

Pla

• Manual: tilt the tube to have a maximum amount of serum (45mn)

• Use synthetic or semi-synthetic swabs (polyester, rayon, nylon) with plastic handle

• Avoid: cotton, calcium alginate swabs and swabs with wooden handles => inactivate virus and inhibit PCR.

• Late shipping => keep sample as frozen organ imprint it on FTA card at the moment of shipment • Refere to sample shipment SOPs

Tr • Only for a local lab

Necrop • Special packaging is required if shipment to foreign lab

• Normal shipment by DHL in an ordinary envelop • Fill accurately the SHS on susy platform

• Mycoplasma: ELISA test should not be done on frozen serum

• Should be shiped frozen with carbonic ice/ice pad

• Avoid hemolysis when you handle blood sample. Do not freeze sera strip

• Normal shipment by DHL in an ordinary envelop.

• Do not touch the strip with finger • Keep the Sera strip ready to use at room temperature

• organs volume = 1/3 of the total volume

• Centrifugation: use Eppendorff tube

• Sera strip: Room temperature

• Hermetically sealed bottles

Serum separation • Liquid serum: 4C° if < 36h

• Avoid the week end and days off

• Well soak the swab deeply in the organ

Mycoplasma

STORAGE CONDITION

BACT

Photos : Ceva / ??????????????????????????????????????????????????

Entire organs

Fresh

FTA cards

Organs Euthanized bird or freshly dead with clinical signs

SAMPLE

PCR

SERO

• Use of monopipette 15µl with disposable tips for serum deposit Ceva Santé Animale S.A. www.ceva.com - contact@ceva.com 10, av. de La Ballastière - 33500 Libourne - France Phone: 00 33 (0) 5 57 55 40 40 - Fax: 00 33 (0) 5 57 55 42 37

HIST

AVIBACTERIUM CORYZA

MG / MS

SALMONELLA

E.COLI

IBD

Disease

• 28 to 35 days • 28-35 days post vaccination

• NewFlend

• Cevac New Flu H9K • Cevac Flu H9K

• 4-6 weeks post vaccination

Troubleshooting/ Status determination

Monitoring of immune response

Troubleshooting • Alive birds

• Lesions, trachea, joint (with lesion) • Trachea, airsac • Serum

• Outbreak: during the outbreak • MG/MS status at 1 DOA, at 6 WOA and before the transfer or lay onset

• Cevac MG F

• Alive birds

• Serum

• 20 birds

• Oropharyngal, choanal cleft, tracheal swab

• 5 days post vaccination

• Vectormune FP MG

Monitoring of immune response

• Corymune (4K &7K)

• > 100 birds to be examinated

• Wing web granuloma

• 7-10 days post vaccination

• S. Gallinarum Pullorum • S. Enteritidis • S. Typhimurium • S. Infantis • ...spp

No Yes

• Infra orbital Sinusal & Laryngal-tracheal swab

Yes (3 tracheas/ swab) Individual serum

******

• 8 to 12 birds (during the outbreak) • 20 serum (20 days after the outbreak)

• Organs swabs • FTA cards • Liquid serum • Sera strip

• Swab

• Visual examination

No Yes Individual serum

• Sockswab • Entire organ • Liquid serum (SPG)

No • Swab: Litter, faecess, Organs (Liver and ovary, spleen, Caeca)

• Swab: Organs (Liver and ovary, spleen, Caeca) • Serum

Yes

• Solid medium swab

• FTA card • Entire frozen organs

Individual serum

Individual serum • Liquid serum • Sera strip

• Liquid serum • Sera strip

• 20 serums (6-7 flocks)

• 5 birds (max 10 flocks)

• 20 serums (6-7 flocks)

• 20 serums

• 8 to 12 swabs • 20 serums

1/ Sick birds or freshly dead to organs sampling • Breeder and layer : 5-8 birds • Broiler: 10 birds 2/ All flocks in the farm: sockswabs 3/ 20 serums /flock for SG

• > 5 DOA: liver, heart, oviduct, ovaries with salmonellosis lesions • < 5 DOA: yolk, liver, caeca and heart • Serum, litter or dust

• During the outbreak (before the use of any antibiotic)

• Cevac Salmovac

Troubleshooting

• 1-2 swabs/sampled site or house

• Sockswab on the litter or 250g of fresh faeces

• 20 serums (4-5 flocks)

• 20 serums

• Serum

• 5 birds/house

• Breeder and layer: 5-8 birds • Broiler: 10 birds • DOC or first-week mortality: 20 birds

From flocks with clinical signs: • 8 or 12 organs (2 or 3 FTA cards) • 2 blood samplings (15-20 days of interval)

• Serum • Caecal tonsile, trachea, spleen • > 5 DOA: liver, heart, oviduct, ovaries with colibacillosis lesion • < 5 DOA yolk, liver and heart • Ompahalitis: Yolk Internal organs: • Liver • Spleen

• 3 days post vaccination

• 3-5 days post vaccination

• First 3 days of the outbreak • Before any antibiotic treatment or 7 days after the end of antibiotic treatment

• Acute phase of the disease • Serotheque

• 20 serums

• 20 serums (4-5 flocks)

• Serum

• 20 serums

• 4-6 weeks post vaccination

• Cevac S. Gallinarum

• Ceva H9 vaccine • Competitor H9 vaccines

• > 30 days, preferably 35-45 days

• Cevac New Flu H9K • Cevac Flu H9K

• Serum

No • Liquid serum • Sera strip

• FTA card • Entire spleen • Feather pulp • 8-12 spleens (2 or 3 FTA cards) • 5 feathers/bird

• 8 spleens (2 FTA cards) • 5 feathers/bird

• Spleen • Feather pulp

• 12 spleens (2 or 3 FTA cards) • 5 feathers/bird

Yes

• Dry swab • Wet swab • FTA cards

Individual serum

No • Liquid serum • Sera strip

• Dry swab • Wet swab • FTA cards

On the flocks with clinical signs: • 8 or 12 organs (2 or 3 FTA cards) • 2 blood samplings (15-20 days of interval)

• 20 serums (6-7 flocks)

• 5 birds per house to obtain 40 swabs

Yes

• FTA card • Entire organs

Individual serum

• Liquid serum • Sera strip

• FTA card • Bursa

Individual serum

POOLING

• FTA card • Entire Spleen • Feather pulp

• Liquid serum • Sera strip

• FTA card • Entire bursa

• Liquid serum • Sera strip

• FTA card • Entire bursa

SAMPLE SUPPORT

• 20 serums

• 20 serums (4-5 flocks)

• 5 birds/house to obtain 30 swabs

On the flocks with clinical signs (2 houses is OK): • 8 or 12 organs (2 or 3 FTA cards) • 2 blood samplings (15-20 days of interval)

• 20 serums (6-7 flocks)

• 20 birds (5 FTA cards)

• 12 birds (5 FTA cards) • Entire organ

• 20 serums (5-7 flocks)

• 20 birds (5 FTA cards)

• 20 serums (5-7 flocks)

• 20 birds (5 FTA cards)

>10 HOUSES/FARM

• Trachea + Kidney + Caecal tonsile • Caecal tonsile + 1 late sera sampling (15 -20 days after the end of clinical signs)

• 20 swabs/farm

On the flocks with clinical signs (2 houses is OK): • 8 or 12 organs (2 or 3 FTA cards) • 2 blood samplings (15-20 days of interval)

• 20 serums (4-5 flocks)

• 12 birds (3 FTA cards)

• 12 birds (3 FTA cards) • Entire organ

• 20 serums (3-5 flocks)

• 13 birds (3 FTA cards)

• 20 serums (4-5 flocks)

• 12 birds (3 FTA cards)

>4 AND <10 HOUSES/FARM

NUMBER OF SAMPLES/FLOCK

On the flocks with clinical signs: • 8 or 12 organs (2 or 3 FTA cards) • 2 blood samplings (15-20 days of interval)

• 20 serums

• 8 birds (2 FTA cards)

• 8 birds (2 FTA cards) • Entire organ

• 20 serums

• 8 birds (2 FTA cards)

• 20 serums

• 8 birds (2 FTA cards)

<3 HOUSES/FARM

• Serum (2x)

• Swab : Choanal cleft, Oropharynx

• Caecal tonsile + Trachea serum (2x)

• Liquid serum • Transport card

• Spleen • Feather pulp

• Bursa

• Blood

• Bursa

• Blood

• Bursa

TYPE OF SAMPLES

• Corymune (4K+7K)

Vaccine monitoring

Troubleshooting

Troubleshooting awareness/survey

Monitoring of immune response

• > 30 days, preferably 35-45 days

• Newflend ND H9

• Acute phase • After outbreak

Vaccine take monitoring (vaccination at Day 1)

Troubleshooting awareness with clinical signs

• Minimal 30 days • Optimal 35-45 days

• 5 days

• Acute phase of the disease • Serotheque

• > 35 days

• 28 to 35 days

• Cevac IBird • Cevac Vitabron • Cevac IBron/Cevac IBras • Cevac MassL/BRON 120 L

• Ceva ND vaccine • Competitor ND vaccines

• Vectormune ND

Monitoring of immune response

Vaccine take monitoring

Troubleshooting awareness/survey

Monitoring of immune response

• Ceva IBD vaccine • Competitor IBD vaccines • Acute phase of the disease

Vaccine take monitoring

Troubleshooting awareness/survey

• 42-49 days

• > 30 days, preferably at slaughterhouse

• 28-37 days

AGE OF SAMPLING

• > 42 days, preferably 49-56 days

• Novamune

• Transmune • Nextmune

TARGETED VACCINE (IF RELEVANT)

Monitoring of immune response

Vaccine take monitoring

Monitoring of immune response

Vaccine take monitoring

OBJECTIVE

Chapter 4. Field support: early health in chicks, ssg, biosecurity, vet program reporting, troubleshooting tools

Brick No. 13 “Troubleshooting tools” Troubleshooting should be seen as a key opportunity to support your customers and improve your partnership. The V.E.T Program is an innovative tool designed to accompany you during the farm visit and the ExpertEye system (smart glasses) can provide realtime distance support between you and experts.

Chapter 4. Field support: early health in chicks, ssg, biosecurity, vet program reporting, troubleshooting tools

Brick No. 18 “Early health” Guidelines on how to audit breeder farms, egg quality, hatchery (DOC quality) and broiler farms. These parts of production need to be investigated to ensure optimal performance of your customers.

Chapter 4. Field support: early health in chicks, ssg, biosecurity, vet program reporting, troubleshooting tools

Brick No. 20 “V.E.T. Program reporting” This brick is considered as a tutorial for the V.E.T Program for your next farm visits and to enable automatic reporting on-site.

Chapter 4. Field support: early health in chicks, ssg, biosecurity, vet program reporting, troubleshooting tools

Brick No. 21 “V.E.T. Program- Asia experience” The bricks are also an opportunity for us to share success stories. A real example of using the V.E.T. program in Indonesia with field visits and customer reports is described here.

Chapter 4. Field support: early health in chicks, ssg, biosecurity, vet program reporting, troubleshooting tools

Brick No. 22 “Biosecurity Guidelines” Here you will find cleaning and disinfection protocols to reduce the presence of pathogens in the farm, describing all the key points to be checked and the basic steps for a good cleaning and disinfection plan.

DOSE

Approved dose

Mineral cleaner (Acid + H2O2)

Disinfectant (Ammonium + Glutaraldehide)

Alkalin cleaner

Water

Clean all surfaces

Rinse all surfaces

Quicklime

Disinfectant 1%homologation

Quicklime

Floor (concrete)

Wall + ceiling

Around the house

SALMONELLA COCCIDIA / HELMINTHS

SITE AND METHOD OF APPLICATION

Setting: high pressure. Rinse all surfaces 30 minutes after detergent application.

Foam gun 300 ml. / m2; contact time 30-40 minutes.

SITE AND METHOD OF APPLICATION

All water pipes. Leave during the whole downtime period. Flush 24 h. prior to DOC placement to avoid foamy water

All water pipes: contact time must be 2-4 h., following flushing.

All water pipes: contact time must be 30-40 minutes, following flushing.

SITE AND METHOD OF APPLICATION

Spray the insecticide solution in the corners, where wall & litter coincide (50 cm. covering the litter and 50 cm. covering the wall)

SITE AND METHOD OF APPLICATION

ü ü

Place the tablet in the silo and let it burn. BEWARE toxic! Mandatory to wear PPE

Spreading on the floor in front of each door and in the cooling pad area

Spray disinfectant solution to humidify everywhere whithout runoff

3 ml disinfectant/ m2

500 g / m2

Put 0,3 to 1 water Liter/m2 to active the quicklime

300 g. / m2

Providor recommandation In tank: cleaner at 2% concentration; 30 minutes; Rinse In second tank: disinfectant 1%; 30 minutes; dry equipment

DOSE

3ml. / m2

Fumigen or disinfectant spray

Cleaner + disinfectant

Diverse elements (feeders / drinkers)

Silo

PRODUCT

WHEN

6- DISINFECTION

PRODUCT

OBJECTIVE

5- CLEANING THE HOUSE

4- LITTER REMOVAL + DUST CLEANING

DOSE

2 L. / 100L water

Biofilm cleaner (Alcalin)

Right after broiler catching (<24h.)

3- REMOVE/DISMOUNT ALL ELEMENTS IN THE HOUSE

1 L. / 100L water

PRODUCT

DOSE

Providor recommandation

USED AS A ROUTINE PRACTICE VIRUSES (IBDV/FAdV/CIRCOVIRUS…)

WHEN

2- DRINKERS AND PIPES

PRODUCT

Adulticide

WHEN

Right after broiler catching

1- DISINSECTION

ü ü

1 bait per trap

DOSE

1 trap each 10 meters

Clean + disinfect farm specific clothes

SITE AND METHOD OF APPLICATION

days! DOSE

Concentrated solution (around 100 L.)

Disinfectant

Rodenticide

1 to 5 ml.. / m3

1 tablet per box

Larvicide (each 2 High dilution (0.5-1 L. / consecutive flocks) m2) Adulticide + 5 g. / bucket attractor

Adulticide

PRODUCT

SITE AND METHOD OF APPLICATION

Quaternary ammonium (detergent) + phenolic compounds (disinfectant).

For Salmonella spp.:

Back to routine situation, if the floor is made of concrete, it can be disinfected normally.

Before quicklime application: Mix 150g. of ammonium sulfate/m2 + 50g. of strengthened sodium hydroxide in a 500 ml. solution (Beware! Dangerous!)

Add 50% of water (use foggers, preferrably); contact time: 10 h.; Volume = 3*Floor surfaces

Complete all boxes and check the farm map with boxes position

1 bucket / 50 m2

Apply on the litter

Spray the reconstituted solution in the corners, where wall & litter coincide and under the feeders

Floor disinfection during cocci / helminths challenge

RECOMMENDATIONS FOR SPECIFIC DISEASE SITUATIONS:

Second disinfection (if needed)

Rodent

Flies

Darkling bettles

WHEN

9- DURING THE TIME BETWEEN NEW LITTER PLACEMENT AND DOC PLACEMENT

Flush the water line and reset the drinker nipples

DOWNTIME PERIOD: Dry out all surfaces; Min. 3

Rodenticide (bait)

PRODUCT

8- BEFORE THE PLACEMENT OF NEW LITTER:

RODENT control

SHOES and CLOTHES

OBJECTIVE

7- SANITARY PROTECTION

Calculate the total surface of the house including all surfaces (including cooling pad, fans, feeders, water lines and sanitary room…): ______________ m². Homologation of disinfectant is 1%è dose of disinfectant will be 3ml/m² Calculate global dose of disinfectant: 3ml X …m²= ….disinfectant liter To mix with water and to spray(humidify) everywhere on all surfaces

PROTOCOL FOR DECONTAMINATION OF BROILER FARMS

Chapter 4. Field support: early health in chicks, ssg, biosecurity, vet program reporting, troubleshooting tools

Example :The Good evaluation of all surfaces is key for HOUSE DECONTAMINATION PROCESS House type+ Floor surface

Developed surface

Surface of equipment

Total surface

Coefficient of surface to disinfect/floor surface

Classical

2800m²

560m²

3400m²

3,4

2800m²

560m²

3860m²

3,9

Finalyze developped Surface calculation

Beton floor 1000m² Classical+ Pad cooling Beton floor

1000m²

500m²

Classical earth floor

1800m²

560m²

2360m²

2,4

1000m² House with nest+ 50%plastic duckboard

3000m²

1000m²

4000m²

House 1000m²with cage 4 stages

6000m²

16000m²

22000m²

House 1000m²with cage 8 stages

10000m²

32000m²

42000m²

House 1000m²with cage 4 stages +padcooling

6000m²

18000m²

24000m²

House 1000m²with cage 8 stages +padcooling

10000m²

34000m²

44000m²

1000m²

Conclusion “Bricks” prove to be an indispensable tool for Ceva poultry veterinarians during their farm visits, and their significance is set to endure over the years. These concise technical sheets provide instant access to vital information, facilitating swift analysis of health (awareness, troubleshooting and monitoring) in poultry farms. The structured format of the “Bricks” allows for efficient identification of potential issues, leading to precise and targeted recommendations and standards. Thanks to their portability, these sheets serve as ideal companions in the field.

Conclusion.

Designed with a long-term perspective, “Bricks” are meant to evolve alongside technical and scientific advancements. Regular updates based on the latest publications and internal data will ensure that the provided information remains current and relevant. This approach will ensure that poultry veterinarians consistently have cuttingedge tools to make informed decisions and promote sustainable poultry health.

In essence, “Bricks” play a pivotal role in the ongoing improvement of poultry practices by offering reliable and up-to-date information. Their adaptability reflects a commitment to excellence in the poultry veterinary field.

We want together to build the brick wall of knowledge to support better and better our customers and to obtain the excellence of Ceva veterinary service.

Conclusion.

Ceva Santé Animale 10 Av. de la Ballastière, 33500 Libourne, Francia Telf. +33 5 57 55 40 40 www.ceva.com