CATALOG 2010 Fixation Processing Embedding Sectioning Staining Storage Instruments
Histo-Line Laboratories Srl is proud to present the New 2010 Histology Catalog as a valuable tool for your clinical diagnostic and research needs. For additional information please visit our website www.histo-online.com and www.histoline.com
Table of contents
Fixation
4
Processing
6
Embedding
8
Sectioning
10
Staining
11
Storage
20
Instruments
22
3
Fixation
Description
Cat.
Quantity
B5 solution
07-119
6 x 500 ml
Bouin solution
07-116
6 x 500 ml
Bouin Holland liquid
07-117
6 x 500 ml
Cyto Spray Cytological Fixative non aerosol
R0150
12 x 250 ml
04018-1 04018-4
1 litre 4 x 1 litre
Glutaraldehyde, Biological grade 25%
00376-500 00376-4
500 ml 4 x 500 ml
Glutaraldehyde, Biological grade 50%
00377-500 00377-1
500 ml 1 litre
Glutaraldehyde, EM grade 8%
07710-100 07710-5 00216-30
100 ml 5 x 100 ml 30 x 10 ml
Glutaraldehyde, EM grade 25%
01909-100 01909-5 01909-10
100 ml 5 x 100 ml 10 x 10 ml
Glutaraldehyde, EM grade 50%
18428-100 18428-5 18428-10
100 ml 5 x 100 ml 10 x 10 ml
Glutaraldehyde, EM grade 70%
01201-2 01201-5
10 x 2 ml 5 x 10 ml
Cyto-Spray is a mixture of ethanol and carbowax specifically formulated to assure rapid, high quality of cytological samples
Formaldehyde Ultra Pure methanol free, EM grade Formaldehyde, 10%, methanol free, Ultra Pure[50-00-0] -Suitable for both electron and light microscopy - Easily penetrates large blocks of tissue - When used in combination with glutaraldehyde, it fixes delicate tissues such as brain in vascular perfusion - Avoids the problem of having to depolymerize paraformaldehyde - Used in Karnovsky's fixative in conjunction with your own buffer system.
Biological Grade Glutaraldehyde has been purified to minimize by-product formation and maximize shelf life. It should be suitable for most morphological studies
4
250 ml 500 ml
Histo-Fix Formalin 10% V/V
DR001 DR002 DR003
4 x 5 litres 10 litres 20 litres
Ice Spray
R0020
12 x 150 ml
Liquid of Carnoy
15-104
250 ml
Paraformaldehyde EM Grade
00380-1 00380-250
1 Kg 250 g
15-123
500 ml
Preserve Your Specimens "To Go" PolyTransport Buffer is a medium used to transport and preserve specimens from surgery to the point of grossing for fixation prior to further processing. The specially formulated transport medium is designed to preserve tissue in a viable state during transport by maintaining osmolarity and pH for 24 to 48 hours.
Fixation
24311-250 24311-250
Histocon PolyTransport Buffer
Freezing Spray aereosol
Paraformaldehyde depolymerizes in water to formaldehyde solution yielding consistent quality fixative solutions. To achieve a strong solution, raise the temperature of the water to 60째C then add sodium hydroxide solution dropwise. Karnovsky, J. Cell Biology, 27, 137A (1965)
Zenker Solution
5
Cat.
Quantity
Blue
R4004
1000 pcs
Grey
R4005
1000 pcs
Green
R4003
1000 pcs
Pink
R4002
1000 pcs
White
R4172
1000 pcs
Yellow
R4001
1000 pcs
White
RBPW
500 pcs
Blue
RBPB
500 pcs
Citro-Histoclear
4 x 5 litres
Clearing agent for Histology, D-Limonene
R0050CITRO
Decalc
R0060
6 X 1 litre
R0055/20
4 x 5 litres
Description Biopty Cassettes
Processing
Disposable cassettes for processing and embedding biopsy
Biopsy Pad
For rapid decalcification with superior staining qualities. Histo-Decalc is a rapid bone and tooth decalcifier for the preparation of small histological materials with improved tissue preservation and superior staining qualities. Decalc represents a major advance in histological technique. The stained preparation shows excellent histological details
Diaphane Clearing reagent
6
Embedding Cassettes R4157
1000 pcs
Green
R4156
1000 pcs
Pink
R4155
1000 pcs
White
R4170
1000 pcs
Yellow
R4154
1000 pcs
Ice Spray
R0020
12 x 150 ml
3410/20
4 x 5 litres
Processing
Blue
Freezing Spray aereosol
Xilene Histo Grade Xilene clearing agent for histology
7
Description
Cat.
Quantity
Base Molds 7x7x5mm
R4161
12 pcs
Base Molds 15x15x5mm
R4162
12 pcs
Base Molds 24x24x5mm
R4163
12 pcs
Base Molds 30x24x5mm
R4164
12 pcs
Base Molds 37x24x5mm
R4165
12 pcs
Base Molds 37x24x12mm
R4166new
12 pcs
Blue
R4004
1000 pcs
Grey
R4005
1000 pcs
Green
R4003
1000 pcs
Pink
R4002
1000 pcs
White
R4172
1000 pcs
Yellow
R4001
1000 pcs
Blue
R4157
1000 pcs
Green
R4156
1000 pcs
Pink
R4155
1000 pcs
White
R4170
1000 pcs
Yellow
R4154
1000 pcs
Base Molds Molds in stainless steel for blocks of paraffin, available in 6 different sizes according the size of the samples. Can be used with Mono Cassettes, Biopty Cassettes and Embedding Rings
Embedding
Biopty Cassettes Disposable cassettes for processing and embedding biopsy
Embedding Cassettes
8
Embedding Rings Embedding Rings are made in ABS plastic material that is heat and compression resistant. ER make it possible to obtain uniform blocks of paraffin and can be set up on any type of Microtome R4013
1000 pcs
Green
R4012
1000 pcs
Pink
R4011
1000 pcs
White
R4151
1000 pcs
Yellow
R4010
1000 pcs
Lab-O-Wax 56-58 °C
R0040
6 x 2 Kg
R0040-PLUS
6 x 2 Kg
R0042
6 x 2 Kg
Mega Cassettes - White
R4173
1000 pcs
Super Mega Cassettes – White
R4173
100 pcs
Embedding Medium for histological use
Lab-O-Wax PLUS 56-58 °C
Embedding
Blue
Lab-O-Wax Plus is a superior embedding media Lab-O-Wax Plus is a mixture of pure paraffin and plastic polymers, compression of tissue due to cutting is reduced to a minimum.
Lab-O-Wax 52-54 °C Mega Embedding Cassettes Cassette for processing and embedding big tissue specimens, is of heat, solvents, decalcification agents and ultrasound proof, plastic material construction.
MEGA
O.C.T. Compound
R0030
12 x 125 ml
9
Disposable Blades
Description
Cat.
Quantity
Disposable Blades Type A35
R4694
50 pcs
R4689
20 pcs
R4691
50 pcs
R4695
50 pcs
Description
Cat.
Quantity
Aqueous Mount
HLAMT030 HLAMT060 HLAMT500
30 ml 60 ml 500 ml
FluoreGuard Mounting Medium
HLFMM030 HLFMM060 HLFMM500
30 ml 60 ml 500 ml
Permanent Mounting Media (Aqueous)
HLPMT030
30 ml
Pertex Mounting Media (Xylene-based)
R0081
6x500 ml
For thin sectioning of paraffin embedded tissue blocks
Disposable Blades Type C35 For sectioning of tissue on cryostat
Disposable Blades Type N35 For sectioning of hard samples
Sectioning
Disposable Blades Type S35 For thin sectioning of paraffin embedded tissue blocks
Mounting Media
10
STAINING SOLUTIONS Description
Cat.
Quantity
EA 50
R0160
6 x 1 litre
Eosin alcoholic
R0190
6 x 1 litre
Eosin aqueous
R0200
6 x 1 litre
Giemsa
R0210 R0215
6 x 500 ml 6 x 1 litre
Haematoxylin Harris
R0180
6 x 1 litre
Haematoxylin Mayer
R0182
6 x 1 litre
May-Grunwald
R0220 R0225
6 x 500 ml 6 x 1 litre
OG2
R0165
6 x 1 litre
OG6
R0170
6 x 1 litre
Staining 11
SPECIAL STAIN KITS
Description
Cat.
Quantity
Kit AgNOR
14-100
1 kit
14-102
1 kit
14-103
1 kit
14-104
1 kit
14-127
1 kit
Method to detect the argentaffin proteins associated to the nucleolar organizing region (NOR).
Kit Alcian blue pH 2,5 Method for the specific demonstration of acid mucopolysaccharides in tissue sections.
Kit Alcian blue PAS
Staining
Method for the simultaneous demonstration of acid mucins, alcian blue positive, neutral mucopolysaccharides and carbohydrates, PAS positive, in tissue sections.
Kit Azan trichrome Method for the demonstration of muscle fibers, glial fibrils, collagen, reticulum, glomerular stroma of the kidney, erythrocytes and nuclear chromatin in tissue sections.
Kit Congo red Method for the demonstration of amyloid in tissue sections. It shows a high birefringence under polarized light.
12
Kit Diastase digestion method
14-105
1 kit
12-103
1 Kit
14-108
1 kit
14-109
1 kit
14-110
1 kit
The digestion on histological section by diastase solution is indicated when the glycogen masks the epithelial mucins. It is a method recommended for liver biopsy
Fast color kit (fast staining of blood smear) Rapid method for differential stain of blood figured elements. Can be used for staining slides of: cytological, fine-needle, cytocentrifuge, malaria..
Kit Feulgen Method for the specific demonstration of DNA in tissue section.
Method for the simultaneous demonstration of bilirubin and collagen in tissue sections.
Kit Fuchsin paraldehyde Gomori
Staining
Kit Fouchet Van Gieson
Method for the demonstration of elastic fibers, alpha and beta cells of islets of Langherans, mast cells granules of gastric mucosa, argentaffin cell granules on tissue sections.
13
Kit Gram
14-111
1 kit
14-112
1 kit
14-113
1 kit
14-115
1 kit
14-116
1 kit
For the simultaneous demonstration of Gram negative and Gram positive bacteria on histological section and smear.
Kit Grimelius Method for the demonstration of argyrophil substances on cells and tissues.
Kit Grocott Method used for the demonstration of certain fungi in tissue section.
Staining
Kit Luxol fast blue Kluwer Barrera Method for the demonstration of myelin and phospholipids on section of central nervous system.
Kit Mallory trichrome Method for the demonstration of collagen, reticulum, cartilage, bone, amyloid and nuclei in tissue sections.
14
Kit Masson Fontana
14-117
1 kit
14-118
1 kit
14-119
1 kit
14-120
1 kit
14-130
1 kit
Method for the demonstration of melanotic pigment on histological section.
Kit Masson trichrome Method for the demonstration of connective tissue; in detail: gametes, nuclei, nevroglia, neurofibrils, collagen, keratin, intracellular fibrils and the negative image of the Golgi apparatus in tissue sections.
Kit Masson trichrome Goldner
Kit May Grunwald Giemsa
Staining
Method for the demonstration of connective tissue; in detail: gametes, nuclei, nevroglia, neurofibrils, collagen, keratin, intracellular fibrils and the negative image of the Golgi apparatus in tissue sections.
Method for the differentiation and demonstration of cell type and parasites, bacteria, rickettsiae, inclusion bodies, mast cell granules and cellular typing and endothelial reticulum in tissue sections.
Kit Methyl Green pyronin Method for the demonstration of DNA and RNA in tissue sections. It is useful also for the demonstration of RNA and plasma-cells on histologic and cytological samples.
15
Kit Nissl for neurons
12-128
1 kit
Kit Oil red O
14-146
1 kit
14-153
1 kit
14-122
1 kit
14-123
1 kit
12-106
1 kit
Method used for neutral lipid demonstration
Kit Orceine for elastic fibres Method for the identification of elastic fibers on tissue section.
Kit PAS Hotchkiss McManus Method for the demonstration of tissue components characterized by adjacent glycol or aminohydroxyl groups.
Staining
Kit PAS Morel Maronger Method for the demonstration of neutral mucopolysaccharides and connective tissue components.
Kit Perls Method for the demonstration of reactive ferric iron on tissue sections.
16
14-124
1 kit
14-125
1 kit
14-107
1 kit
14-128
1 kit
Kit Sudan black
12-105
1 kit
Kit Van Gieson trichrome
14-129
1 kit
Kit Perls Van Gieson Method for the demonstration of reactive ferric iron, collagen and connective on tissue sections
Kit Picromallory For the demonstration of the different connective components.
Kit PTAH phosphotungstic haematoxylin The method is useful in order to differentiate the skeletal muscle from the smooth one: it stain the isotropic bands of the . skeletal muscle. It points out also the fibrin.
For the demonstration of tissue argyrophil tissue components, glycogen and mucines. It is widely used for the demonstration of certain fungi in tissue section. It is an elective method for the study of basement membrane in kidney biopsy.
Staining
Kit Silver methenamine P.A.S.M
Method for the demonstration of collagen fibers, discriminates them from connective tissue.
17
Kit Von Kossa
14-131
1 kit
14-132
1 kit
14-133
1 kit
14-134
1 kit
14-135
1 kit
Method for the demonstration of anions of calcium salts in tissue section.
Kit Warthin Starry Method for the demonstration of spirochete on tissue section.
Kit Weigert's long method For the demonstration of elastic fibers in tissue sections. This method uses overnight staining and it is preferred for very fine fibers staining.
Staining
Kit Weigert's fast Method for the demonstration of elastic fibers in tissue sections. This short method has a lower specificity: also collagen and basal membrane could be stained.
Kit Weigert's Van Gieson The stain method is particularly useful for the demonstration on the same slide of elastic fibers, connective tissue, collagen and nuclei. It is suitable for vascular pathology studies.
18
Kit Weigert's Van Gieson fast
14-136
1 kit
14-137
1 kit
14-138
1 kit
Method for the demonstration of elastic fibers in tissue sections, many variants exist of the original Weigert’s method, this short method has a lower specificity: also collagen and basal membrane could be stained.
Kit Ziehl-Neelsen Method for the demonstration of pathological mycobacteria in tissue section, in sputum or in culture media smears. Particularly for Kock’s and Hansen’s bacteria.
Kit Ziehl-Neelsen Fite
Staining
Method for the demonstration of pathological mycobacteria in tissue section, in sputum or in culture media smears. Particularly for Kock’s and Hansen’s bacteria.
19
FILING SYSTEMS FOR PARAFFIN BLOCKS
Description
Cat.
Qty
Filing Cabinet WHITE in METAL for paraffin blocks
R4192
1
Filing Cabinet WHITE in ABS for paraffin blocks
R4192-ABS
1
Filing Cabinet BLUE in ABS for paraffin blocks
R4192A-ABS
1
Filing Cabinet GREEN in ABS for paraffin blocks
R4192G-ABS
1
Filing Cabinet HAVANA in ABS for paraffin blocks
R4192H-ABS
1
Filing Cabinet PINK in ABS for paraffin blocks
R4192R-ABS
1
Filing Cabinet YELLOW in ABS for paraffin blocks
R4192Y-ABS
1
Filing Cabinet for Macro Cassettes
R4192/MACR O
1
Base stand for Filing Cabinet
R0549
1
Top Cover for Filing Cabinet R41 and R054
R0548
1
Storage 20
FILING SYSTEMS FOR SLIDES
Description
Cat.
Quantity
Filing Cabinet WHITE in METAL for slides
R0540
1
Filing Cabinet WHITE in ABS for slides
R0540-ABS
1
Filing Cabinet BLUE in ABS for slides
R0540B-ABS
1
Filing Cabinet GREEN in ABS for slides
R0540G-ABS
1
Filing Cabinet HAVANA in ABS for slides
R0540H-ABS
1
Filing Cabinet PINK in ABS for slides
R0540R-ABS
1
Filing Cabinet YELLOW in ABS for slides
R0540Y-ABS
1
Base stand for Filing Cabinet
R0549
1
Top Cover for Filing Cabinet
R0548
1
Storage 21
TEC-2800
Embedding Center
Characteristics: Working surface The tissue embedding cassettes and base molds trays are removable and can be set at different temperatures The heated forceps holder can be set at the preferred temperature. The ergonomic large working surface allows processing many samples at the same time The setting knobs allow to adjust the precise paraffin flow Cold Spot The large cold spot Peltier is designed to orient even small and big samples (Mega Cassettes) The temperature range is from room temp. to -5 째C The large work surface allows draining of the excess paraffin into two large collecting trays and allows processing many samples at the same time The temperature can be set from ambient to +99 째C and can be adjusted through the control panel Control Panel All functions of the TEC 2800 are settable through the control panel Temperature of working surface Temperature of molds cassettes warming trays Temperature of paraffin tank Work time and day can be programmed automatically
Instruments
Nominal supply voltage Maximum power draw Working temperature increments Capacity Paraffin reservoir Cassettes/ molds tray Dimensions Width Depth Height Weight
220 V/50 Hz 1000 VA from ambient to 70 째C 1째C 4,5 Lt 1,4 Lt 560 mm 640 mm 400 mm 26 Kg
22
TEC 2800 Cryo Console The TEC 2800 Cryo Console is composed of a compressor cooled work surface held to chill the paraffin blocks rapidly. The Teflon coated surface allows a minimum ice formation and easy cleaning. Temperature is adjustable from ambient to -13 °C. TEC 2800 Cryo Console can be used as a stand alone unit near the microtome station. Nominal supply voltage Maximum power draw Working temperature increments Dimensions Width Depth Height Weight
220 V/50 Hz 110 VA from ambient to -13 °C 1 °C 340 mm 600 mm 390 mm 24 Kg
TEC 2800 Thermal Console The TEC 2800 Thermal Console holds processed tissue specimens, in any type of cassettes, awaiting paraffin embedment. Stores and warms samples in a large paraffin tank “2,5 Lt.” Temperature can be set from ambient to 99 °C. 220 V/ 50 Hz 400 VA from ambient to 70 °C 1 °C 2,5 Lt 300 mm 540 mm 390 mm 7,5 Kg
TEC 2900 WaxTrim The TEC 2800 Histo Trim is a practical device designed to remove excess of paraffin from the embedding cassettes after inclusion of specimens. Scraping the embedded block on the “grooved plane” heated surface, excess paraffin will be removed leaving the cassette clean ready to be set properly on the clamp of the microtome. Temperature can be set from ambient to 99 °C.
Instruments
Nominal supply voltage Maximum power draw Working temperature increments Capacity Paraffin tray Dimensions Width Depth Height Weight
23
ARM 3600
Fully Motorized Programmable Rotary Microtome
Function and characteristics: Motorized drive Compatible Embedding Material: paraffin, plastic The cutting and trimming function can be finished by the control system The hand wheel can be locked at any position, so as to ensure the safety of the operation The large waste tray can be exchanged arbitrarily Two kinds of the specimen clamps, standard and universal cassette can be changed easily Two models for section: Manual and automatic, the user can switch arbitrarily. Switch arbitrarily between section and trimming Speed control through the knob for enhanced efficiency Intuitive control panel Section thickness totalized and section counter New blade holder with finger safe Wide range of accessories
Standard delivery: Standard delivery include:
Instruments
1 ARM 3600 basic instrument 1 section waste tray 1 disposable blades holder 1 universal fast clamp for cassette 1 standard clamp for blocks 1 operating manual 1 bottle (50 ml) of oil for drives 1 box of 50 disposable blades type S35 Do not include: 1 holder for standard reusable knife 1 stainless steel knife
24
Technical Parameters: Approvals:
CE IVD
Room conditions Room temperature range: Relative humidity:
+ 15 °C to + 35 °C max. 80% , non condensing
Microtome Section thickness setting:
0,5 - 100 µm 0 to 10 µm in 0,5 µm increments 10 to 20 µm in 2 µm increments 20 to 100 µm in 5 µm increments 1 – 100 µm 1 to 10 µm in 1 µm increments 10 to 20 µm in 2 µm increments 20 to 50 µm in 5 µm increments 50 to 100 µm in 10 µm increments
Maximum section of slice: Slice precision:
50×50mm ±10%
Horizontal specimen stroke: Vertical specimen stroke: Retraction of the specimen clamp: Orientation of the specimen head:
26 mm 60 mm 20 m 360°
Dimensions Width (with handwheel): Width (without handwheel): Depth (with waste tray): Depth (without waste tray): Working height (knife edge): Working height (knife edge): Height (total): Weight (without accessories):
413 mm 300 mm 618 mm 520 mm 100 mm 168 mm 305 mm (with storage area on the housing) approx. 37 kg
Instruments
Trimming section thickness setting:
25
MRS 3500
Motorized Semiautomatic Utra-Thin Microtome
Function and characteristics: Motorized drive Compatible Embedding Material: paraffin, plastic. The cutting and trimming function can be finished by the control system The hand wheel can be locked at any position, so as to ensure the safety of the operation The large waste tray can be exchanged arbitrarily Two kinds of the specimen clamps, standard and universal cassette can be changed easily Switch arbitrarily between section and trimming Intuitive control panel Section thickness totalize and section counter New blade holder with finger safe Wide range of accessories
Standard delivery: Standard delivery include:
Instruments
1 MRS3500 basic instrument 1 section waste tray 1 disposable blades holder 1 universal fast clamp for cassette 1 standard clamp for blocks 1 operating manual 1 bottle (50 ml) of oil for drives 1 box of 50 disposable blades type S35 Do not include Optional: 1 holder for standard reusable knife 1 stainless steel knife
26
Technical Parameters: Approvals:
CE IVD
Room conditions Room temperature range:
+ 5 °C to + 40 °C
MRS3500 Section thickness setting:
0,25 - 60 µm 0.25 to 1 µm in 0.25 µm increments 1 to 10 µm in 1 µm increments 10 to 20 µm in 2 µm increments 20 to 60 µm in 5 µm increments
Horizontal specimen feed: Vertical stroke: Specimen orientation (optional) Horizontal: Vertical:
28 mm 60 mm
Maximum specimen size:
W 50 x H 66 x D 30 mm
Dimensions Width (with handwheel): Depth (with waste tray): Height: Weight (without accessories):
305 mm 445 mm 310 mm 26 kg
± 25 mm ± 20 mm
Instruments 27
MR 2258
Manual Rotary Microtome
Function and characteristics: Compatible Embedding Material: paraffin, plastic The hand wheel can be locked at any position, so as to ensure the safety of the operation The large waste tray can be Exchanged arbitrarily Two kinds of the specimen clamps, standard and universal cassette can be changed easily New blade holder with finger safe Wide range of accessories
Standard delivery: Standard delivery include: 1 MR 2258 basic instrument 1 section waste tray 1 disposable blades holder 1 universal fast clamp for cassette 1 standard clamp for blocks 1 operating manual 1 bottle (50 ml) of oil for drives 1 box of 50 disposable blades type S35 Do not include Optional:
Instruments
1 holder for standard reusable knife 1 stainless steel knife
28
Technical Parameters: Approvals:
CE IVD
Room conditions Room temperature range:
+ 5 °C to + 40 °C
MR2258 Section thickness setting:
1 - 60 µm 0,5 to 2 µm in 0,5 µm increments 2 to 10 µm in 1 µm increments 10 to 20 µm in 2 µm increments 20 to 60 µm in 5 µm increments 25 mm ±1 mm 60 mm 8° 8° ± 90 °
Dimensions Width (with handwheel): Depth (with waste tray): Height: Weight (without accessories):
400 mm 600 mm 300 mm 34 kg
Instruments
Horizontal specimen feed: Vertical stroke: Specimen orientation (optional) Horizontal: Vertical: Rotability:
29
MC 4000
Microtome Cryostat Dual Compressor UVC Lamp Decontamination Automatic Defrosting
Function and characteristics: Motorized drive Chamber temperature down to –30°C Dual Compressor unit for rapid freezing down to –50°C Automatic and manual defrosting The cutting and trimming function can be finished by the control system Holder for disposable blades with adjustable cutting angle Anti roll device The hand wheel can be locked at any position, so as to ensure the safety of the operation Switch arbitrarily between section and trimming Intuitive control panel Section thickness totalize and section counter New blade holder with finger safe Wide range of accessories
Standard delivery include:
Instruments
1 MC 4000 basic instrument 1 section waste tray 1 disposable blades holder 5 Specimen discs 1 Heater extractor 1 operating manual 1 box of 50 disposable blades
30
Technical Specifications: Section thickness setting
1-60 µm 0-10 µm in 1 µm increments 10-20 µm in 2 µm increments 20-60 µm in 5 µm increments
Maximum specimen size: Horizontal specimen feed: Vertcal specimen stroke: Specimen orientation : Trimming:
50x70 mm 28 mm 59 mm 8° (x,y,z axis) motorized coarse feed
Motorized coarse feed: Rapid: Slow:
2 speeds 0,8mm/sec 0,2 mm/sec
Dimension: Width (excluding handwel): Width (including handwel): Depth: Height: Weight:
705 mm 760 mm 640 mm 1115 mm 152 Kg
Instruments
Cooling capacity: Temperature setting range: 0°C to –35°C Cooling down time to –35°C appr. 4 hours Chamber defrosting programmable or manual 1 automatic defrosting cycle / 24 hour duration : 9 min. Freeze shelf temperature -40°C Nominal supply voltage: 220 V / 50 Hz 110 V / 60 Hz Maximum power draw: 450 VA
31
MC 5000
Microtome Cryostat Dual Freezing System UVC Lamp Decontamination Automatic Defrosting
Function and characteristics: Motorized drive. Chamber temperature down to –35°C Double Peltier unit for rapid freezing down to –50°C Automatic and manual defrosting The cutting and trimming function can be finished by the control system. Holder for disposable blades with adjustable cutting angle Anti roll device The hand wheel can be locked at any position, so as to ensure the safety of the operation. The specimen clamp can be oriented on the X—Y axis Section waste aspiration system. Switch arbitrarily between section and trimming. Intuitive control panel Section thickness totalize and section counter New blade holder with finger safe Wide range of accessories
Standard delivery include:
Instruments
1 MC 5000 basic instrument 1 section waste tray 1 disposable blades holder 5 Specimen discs 1 Aspiration hose 1 Heater extractor 1 operating manual 1 box of 50 disposable blades
32
Technical Specifications: CE IVD
Microtome: Section thickness range: Increment: Trimming thickness range: Increment: Specimen retraction: Horizontal stroke: Vertical stroke: Specimen orientation:
1-60 m Adjustable 1 m, Increment: ±20% 10 - 300 m Adjustable 10 m, Increment:±20% 20 m 25mm 45mm 8 X,Y,Z three directions
Cryostat Voltage: Frequency: Power: Chamber temperature: Freezing shelf temperature: Number of freezing station: Peltier number: Refrigerant: Compressor oil:
220V±10% 50Hz 850 V/A -10 - -35 Adjustable -50 18 2 R404a,300g±10g 0.6L EMKARATE RL-22S, ICI
Dimensions: Width (no hand wheel): Width With hand wheel): Depth: Height: Weight:
680 mm 826 mm 700 mm 1150 mm 126 kg
Instruments
Approvals:
33
ATP 700
Automatic Tissue Processor
Function and characteristics: Compact, bench top, linear tissue processor designed to process biological tissues from fixation to paraffin infiltration The unique linear design offers reliable technique in a small space in the laboratory Histo Line ATP 700 includes 14trays, 10 reagents and 4 paraffin can process 2 x 75 samples simultaneously All the reagent and paraffin containers are completely closed to prevent evaporation Electrostatic filter prevent solvent odors and pollution ATP 700 can be programmed for immediate or delayed start The immersion time can be set from 1 minute to 99 hours and 59 minutes
Instruments 34
Technical Specifications: 14 4 6 150 1500 ml
Setting range time for each step: Temperature range for paraffin:
From 1 min. to 99 h 59 min. From 50째C to 89째C increments 1째C
Up and down agitation : Delay time setting:
4 times every 5 min. 99h 99 min.
UPS:
Outside
Nominal supply voltage: Maximum power draw:
220 V / 50 Hz 110 V / 60 Hz 1000 VA
Dimension: Width: Depth: Height: Weight:
1400 mm 500 mm 550 mm 142 Kg
Instruments
Number of Stations: Number of Paraffin Stations Number of stored programs: Number of samples in process: Capacity of single tray:
35
Histo-Pro 200
Vacuum Tissue Processor Extremely Reliable
Technical Data: Nominal voltage: AC220V Nominal frequency: 60HZ Main fuses: 10A Nominal power: 1000 VA Dry weight, unpacked: approx. 100 kg Weight, packed: 120 kg Operating temperature range: 15 °C to 35 °C Relative humidity: 10 to 80 %, Paraffin wax baths Number of baths: 3 Volume (liters): 4.0 l per bath Melt time: approx. 3 hrs Temperature: 65 Temperature accuracy: + 1 Retort Capacity: approx. 200 cassettes Reagent volume : 5.3 Lt. Temperature (paraffin): 65 °C Temperature (reagents): ambient or 35°C Temperature (cleaning reagents): 65 °C Temperature accuracy: + 1 °C Filling time: 5 min Drain time: 5 min Impregnation vacuum: -70 kPa (g) Impregnation pressure: 35 kPa (g) Fill vacuum: -70 kPa (g) Drain pressure: 35 kPa (g)
General Reagent bottles: 9 Cleaning solution bottles: 3 Maximum bottle volume: 4.0 l Recirculation (pump in/out): ON/OFF Time between cycles: 15 min Hardware and Software: Large color Liquid Crystal Display (LCD) User-friendly, intelligent software. Alarm system to reminder wrong operation and troubles Multiple specimen protection system. Capacities: 15 programs that consist of up to 9 reagent and 3 paraffin wax steps. Time per program step: 0 to 99 hours, 59 minutes. Up to 200 cassettes can be processed Wax clean program. 9 reagent bottles. 3 wax baths. 3 cleaning solution bottles. 2 condensate bottle.
Instruments 36
SA3000 Series
Biological Microscope
Specifications: Magnification Objective / eyepiece
4X
10X
40X
100X (oil)
wide field high-eye 10X
40X
100X
400X
1000X
64X
160X
640X
1600X
point
WF 16X
Mechanical stage size of stage: 142mmX 132mm moving range: 55mm X 75mm Ocular head Monocular: incline 45° rotational 360° Binocular: incline 45° rotational 360° Dual View: incline 30° rotational 360° Trinocular: incline 30° rotational 360° Power supply (1) universal input voltage: 100V-240V (2) output voltage: 6V-20W (3) fuse: 1.5A/ Ø5 x 20 item ocular head
SA3100
binocular incline 45 °
Monocular incline 45°
SA3200 dual view incline 30°
SA3300 Trinocular incline 30°
rotational 360° eyepiece
wide field view and high-eye point eyepiece 10X/18, P 16X
objective
Achromatic objective 4X, 10X, 40X, 100X (oil)
stage coarse/fine focusing system condenser illumination system
double mechanical stage: 142mmX 132mm, moving range: 55mm x 75mm coaxial coarse and fine system adjustment arrange 0.002mm20mm
Instruments
SA3000
Kohler type, condenser with variable diaphragm, the adjustment of rising and falling build-in halogen light: 6V/20W
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Cito-Spin
Cytology Centrifuge
Features : Cytological centrifuge is controlled by microprocessor Program save and recall up to ten protocols Gentle stop and fast braking : pre select 10 braking rates Warning indicators for imbalance, over speed, door open, setting failure. Rotor imbalance is detected and immediately signaled to operator
Instruments
Specification: Max RPM : Max RCF : Maximum Capacity : Timer : Braking rates: Programmability: Digital display : Drive Motor : Power : Dimension:3 Specimen range: Weight:
3,000 rpm 1,057 x g 12 Slide x 6cc 99min 59sec or "free" for long run 10 setting 10 memory Speed, Time, Brake, Program High torque DC Motor 220V, 50/60Hz, Single Phase, 0.5Kw C. 60 W x 410 D x 280 H mm. 0,5 to 6 cc. 24 Kg.
Accessories: Sample clip angle Rotor : Max RPM : Max Capacity : Cell : Rubber plate : Cell Holder :
1 set 3,000 rpm Max RCF : 1,057 x g 12 Slide x 6cc 6cc (for 14x14mm) 12ea/set 6cc (for 14x14mm) 12ea/set 12ea/set
1set 1set 1set
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Distributed by:
Histo Line Laboratories S.r.l. Via Brembo,27-20139 Milano ITALY. Tel:+39 0255230061 Fax:+39 0255213764 Email: histoline@histoline.com URL: www.histo-online.com - www.histoline.com