Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression

Page 1

Brown JC, J Genet Genomic Sci 2022, 7: 034 DOI: 10.24966/GGS-2485/100033

HSOA Journal of

Genetics & Genomic Sciences Research Article

Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression Jay C Brown* Department of Microbiology, Immunology and Cancer Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA

Abstract The study described here was pursued with the idea that information about control of human gene expression might result from comparing the promoter regions of a highly expressed gene population with that of a related population that is weakly expressed. Systematic differences in the two promoter populations would be candidates for a role in causing the distinct expression levels observed. The results reported here were obtained with human genes encoding Transcription Factors (TF). Among TF genes, those with wide tissue transcription are strongly expressed while those expressed in one or only a few tissues are weakly transcribed in most cases. The promoter regions of the two populations were compared using the results of ChIP-seq experiments. Results demonstrated that the promoter region of broadly expressed TF genes is enriched in binding sites for POLR2A, a component of RNA polymerase II while promoters of tissue targeted genes are enriched in EZH2, a subunit of Polycomb Repressive Complex 2 (PRC2). It was rare to observe promoters with binding sites for both POLR2A and EZH2 or for neither one. The findings are interpreted to indicate that strong expression of broadly expressed TF genes is due to the presence of RNA polymerase II at the promoter while weak expression of tissue targeted promoters results from the presence of PRC2. Finally, transcription factor families were compared in the proportion of broadly expressed and tissue targeted genes. The results demonstrated that most families possess both broadly expressed and tissue targeted members. For instance, this was the case with 16 of 20 TF families. The results are interpreted to indicate that while individual TFs such as EZH2 may be specific for broadly expressed or tissue targeted genes, this is not a property of most TF families. Most have both broadly expressed and tissue targeted members. *Corresponding author: Jay C Brown, Department of Microbiology, Immunology and Cancer Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA, Tel: +1 4349241814; E-mail: jcb2g@virginia.edu Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034. Received: May 09, 2022; Accepted: May 23, 2022; Published: May 30, 2022 Copyright: © 2022 Brown JC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Introduction Studies on the control of gene expression have benefitted from recent advances in DNA sequencing and from identification of biochemical systems that result in up- or down-regulation of transcription. Whole genome sequences are now available for a wide variety of organisms including humans, and well-characterized elements of regulatory control include promoters, epigenetic signaling, CpG islands, structured chromosome domains, mRNA splicing and many others [1-4]. In view of the variety of regulatory pathways found in higher vertebrates, it is unreasonable to assume that there is an overall regulatory system that applies to all genes. More likely is the idea that distinct regulatory regimes apply to distinct gene populations with the effects of individual regulatory systems integrated to create the overall developmental program observed. In view of the environment of regulatory studies described above, it is reasonable to suggest that progress in understanding regulatory control might be made by comparing the control elements of two populations of related genes that differ in their level of expression. The idea is that such a comparison might lead to information about the identity of the regulatory features involved, at least in a restricted group of genes. Here I describe the results of a study employing the strategy described above and focused on the genes encoding human Transcription Factors (TF). Transcription factors expressed in a wide range of tissues (broadly expressed genes) were found to be highly expressed compared to most tissue targeted TF genes where transcription is weaker. TF expression levels were assessed by the results of RNAseq experiments while ChIP-seq studies were examined to identify protein binding sites in the gene promoter. The results are interpreted to support the view that Polycomb Repressive Complex 2 (PRC2) plays a central role in regulating the expression of human TF genes, particularly those of weakly expressed tissue targeted genes. PRC are multi-subunit molecular complexes able to bind the promoter region of a target gene and suppress the gene’s expression [5-6]. First discovered in Drosophila in 1947 [7], PRC functions have been actively studied ever since. PRC or components of PRC are found in all metazoans and in some more primitive species including fungi. Two forms of PRC complex are known, PRC1 and PRC2, and both can introduce covalent modifications into the histone components of chromatin. PRC1 ubiquitinates H2 at lysine119 [8] while PRC2 methylates H3 at lysine27 [9-12]. PRC1 and PRC2 are thought to function together to repress gene expression and/ or to report on the expression due to other control factors.

Materials and Methods Gene databases All human transcription factor genes (Supplementary Table 1; 1018 genes): This database was derived from the list of Vaquerizas et al., [13] with a modification involving zinc finger transcription factor genes. ZNF genes were examined individually using GeneCards

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

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(https://www.genecards.org) and NCBI Gene (https://www.ncbi.nlm. nih.gov). A ZNF gene was retained in the database only if its role as a transcription factor was supported by an experimental study. Sample of all human genes (SupplementaryTable 2, 183 genes): A sample of all human protein coding genes was accumulated to serve as a control for analysis of transcription factor genes. Included genes begin with ACO1 and proceed rightward on chromosome 9 for a total of 183 protein coding genes. Sample of testis-specific genes (Supplementary Table 3, 204 genes): This database was curated by Brown [14]. Database of all human transcription factor genes showing predominant structural features present in the TF protein (Supplementary Table 4, 1018 genes): Structural element annotations were obtained from GeneCards and NCBI Gene.

RNA-seq and ChIP-seq results for transcription factor and control genes Transcription levels for all genes reported here were obtained from the GTEx Portal of RNA-seq results as reported in the UCSC Genome Browser (version hg38 (https://genome.ucsc.edu/ )). A gene was annotated as “broadly expressed” (B in Supplementary Table 1) if it was found to be expressed in 90% or more of the 52 tissues reported by GTEx. Otherwise, the gene was annotated as “tissue targeted” (T). Borderline genes were rare (10 of 1018 genes). They are indicated by B/T or T/B in supplementary table 1 depending on the category they most resemble. Transcription levels for broadly expressed genes were the average of the top 5 tissues reported by GTEx. For tissue targeted genes, reported values were those of the tissue specific gene or the average of the top 5 tissues in cases where more than one tissue was in the group. Transcription factor binding sites in the promoter region of transcription factor genes were derived from ChIP-seq studies reported in the ENCODE project database of cis-Regulatory Elements (3 November 2018 version) by way of the UCSC Genome Browser. Visual scanning of promoter binding sites was done with UCSC Genome Browser. An EZH2 entry was included in supplementary table 1 if it was present in 50% or more of the studies reported by ENCODE. Values for the level of EZH2 promoter binding were those reported by the Integrated Genomics Viewer (https://igv.org/app/); the cells and study were GM12878 and ENCFF273NDY, respectively. Only binding sites in the promoter region are reported here; sites in non-promoter regions were ignored.

Transcription factor families and protein structural features These were derived from GeneCards and from NCBI Gene.

Data handling Data were manipulated with RStudio and Excel. Graphic rendering was done with SigmaPlot v14.5.

Results Broadly expressed and tissue targeted human TF genes The project was carried out beginning with a database of 1018 human transcription factor genes (Supplementary Table 1). The database J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

was that of Vaquerizas et al., [13] with the modifications described in Materials and Methods. Each gene was classified as either broadly expressed or tissue targeted using information about its tissue expression in the RNA-seq results reported by GTEx Portal via the UCSC Genome Browser (https://genome.ucsc.edu/). Of the 1018 database genes, 589 (58%) were classified as broadly expressed and 419 (41%) as tissue targeted (Supplementary Table 1, B/T column).

Gene expression in broadly expressed and tissue targeted TF databases It was expected that broadly expressed TF genes would be expressed at a higher level than that of tissue targeted TF genes because of the need for expression of tissue targeted TF’s to be suppressed in some tissues. This expectation was tested by rank ordering each population according to the expression level of each gene. The two populations were then plotted together so that the overall expression level could be compared. The results (Figure 1) showed that tissue targeted TF genes are expressed at a lower level than broadly expressed ones, particularly among the genes with the lowest transcription levels. Above a rank of ~180, expression of the two populations was more equal with the tissue targeted population exceeding the broadly expressed genes at the highest expression levels (Figure 1). It was concluded that comparison of the two populations was appropriate for the studies contemplated here.

Figure 1: Expression level of broadly expressed compared to tissue targeted human transcription factors. The expression levels of genes in the two populations were ordered by level and compared by plotting on the same graph. Note that the level of tissue targeted TF expression is less compared to broadly expressed genes, particularly in low expression range.

TF binding sites in the promoter region of TF genes TF binding sites were identified using ChIP-seq results available from ENCODE. At first, identified sites in graphical form were examined visually in the UCSC Genome Browser. Images revealed that binding sites for POLR2A and EZH2 were asymmetrically distributed in broadly expressed compared to tissue targeted TF gene promoters. For instance, among 589 broadly expressed TF genes, 499 (85%) had one or more POLR2A binding sites in the promoter while among 419 tissue targeted TF genes, 246 (59%) had binding sites for EZH2 (Figure 2a). It was rare to find EZH2 binding sites in broadly expressed gene promoters (7%) or POLR2A in tissue targeted genes (12%; see Figure 2a). It was also rare to find TF gene promoters that lacked both POLR2A and EZH2. Of the 1018 genes examined, only 54 (5%) lacked binding sites for both (Figure 2a). Volume 7 • Issue 1 • 100034

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of human protein coding genes. Is asymmetry observed only in TF genes, or does it exist more widely? To test the idea, the experiment described above was repeated beginning with an unselected population of all human protein coding genes rather than with TF genes only (Supplementary Table 2). The results showed that among 141 unselected, broadly expressed genes, 127 (91%) had POLR2A in the promoter while 8 (6%) had EZH2, a result comparable to the TF gene population. In contrast, in the tissue targeted population the results were 40% and 29% for POLR2A and EZH2, respectively, a result quite different from the TF genes (Fig. 2b). The results are interpreted to support the view that the POLR2A/EZH2 asymmetry is found in TF genes only and is not found in an unselected gene population.

Control: POLR2A and EZH2 binding sites in testis-specific genes A further control study was carried out to examine POLR2A and EZH2 binding in the promoters of a population of tissue targeted genes, a population of 204 testis-specific genes (Supplementary Table 3). ChIP-seq results, as described above, were examined in the promoter regions of the testis-specific genes. It was expected that EZH2 binding sites would be observed in a high proportion of genes if testis-specific genes were regulated in the same way found for tissue targeted TF genes. Quite a different outcome was detected (Figure 2c). Promoters of testis-specific genes were not found to be enriched in either POLR2A or EZH2. Instead, the promoters had a mixture of transcription factor binding sites in which no predominant species could be identified (Supplementary Table 3). For instance, of 195 testis-specific genes that yielded a result, 135 (69%) had neither a POLR2A nor an EZH2 site. POLR2A and EZH2 were found in 22% and 9%, respectively (Fig. 2c). The result is interpreted to indicate that while EZH2 binding sites are prevalent in the promoters of tissue targeted TF genes, the same is not found in other populations of tissue targeted genes.

EZH2 represses TF gene expression as a component of the polycomb repressive complex 2

Figure 2: POLR2A and EZH2 binding sites in the promoter regions of broadly expressed and tissue targeted genes. Results are shown for (a) all database human transcription factor genes; (b) an unselected population of human protein coding genes; and (c) a sample of human testis-specific genes. Note that EZH2 binding sites (indicative of PRC2 binding) are prominent in tissue targeted TF genes, but not in broadly expressed TF genes (a), a mixed population of human protein coding genes (b) or in testis-specific human genes (c).

Control: POLR2A and EZH2 binding sites in the promoters of unselected human protein coding genes As a control, an experiment was performed to test whether the asymmetry observed between POLR2A and EZH2 in the promoter region of TF genes would also be observed in an unselected population J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Binding of EZH2 to the promoter regions of tissue targeted TF genes as described above was interpreted to indicate that EZH2 acts to repress TF gene expression as a component of PRC2, a complex known to be involved in gene repression [5-6]. EZH2 is a prominent component of PRC2, and PCR2 introduces repressive modifications into chromatin. To test the idea that EZH2/PCR2 acts to repress TF gene expression, the expression level of tissue targeted TF genes was plotted against the amount of EZH2 reported in the TF gene promoter (Figure 3). Creation of the plot was complicated by the fact that as EZH2 is repressive, it was difficult to find an appropriate population of tissue targeted TF genes. Most were expressed to only a background level. Fig. 3 shows the results of all 24 of the genes with transcription levels above background. The regression line in the plot indicates that the amount of promoter bound EZH2 is corelated with lower rather than higher gene expression. The results support the conclusion that EZH2 is repressive for its target TF gene population. The results are also compatible with the idea that EZH2 acts as a part of the PRC2 complex.

Preferred levels of EZH2 bound at target promoters The availability of quantitative values for the level of EZH2/PRC2 in the promoters of tissue targeted TF genes enabled a determination Volume 7 • Issue 1 • 100034

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The above result was not expected. Preferred spacing was not observed when similar histogram analysis was performed with gene expression values from the same dataset. The observation (Figure 4) is interpreted to indicate that EZH2/PRC2 complexes are added to promoter regions in units containing two or more EZH2 molecules. Preferred spacing arises when more than one multi-EZH2/PRC2 complex attaches to a promoter.

Transcription factor families

Figure 3: Plot showing transcription factor expression level as a function of EZH2 protein bound to the promoter. Note that increased EZH2 binding correlates with decreased TF gene expression. Data points corresponding to a few individual TFs are noted on the plot.

The information above indicating that promoter binding sites for EZH2 are located preferentially in tissue targeted TF genes raises the question of whether there may be families of TFs whose members have a selectivity for controlling tissue targeted TF genes only, or broadly expressed TF genes only. Could binding to tissue targeted promoters be a property of a TF family and not only of an individual TF? To explore the above question, the TF family of each TF employed here was recorded and the families were characterized according to the number of broadly expressed and tissue targeted genes they contain. All results are shown in supplementary table 4 with a sample of 20 families shown graphically in figure 5.

of whether the collection of values is a continuous function or whether there exist preferred levels. The issue was examined beginning with all 246 database TF genes that were judged to be tissue targeted and also to be enriched in EZH2 binding in the promoter (Supplementary Table 1, EZH2 column). A histogram was plotted relating the amount of EZH2 bound at the promoter and the number of times a similar value was found among the 246 genes (Figure 4). The plot shows evidence of preferred binding levels in promoter associated EZH2. At least four preferred complexes were identified (at 4,8,12 and 16 EZH2 units), and spacing was found to be ~4 in the units provided by the ChIP-seq analysis (signal p-value).

Figure 5: Number of broadly expressed and tissue targeted TFs in selected TF families. See supplementary table 4 for all results. Note that most families contain both broadly expressed and tissue targeted TF genes. Families shown: ATF, activating TF; CREB, cAMP response element binding TF; E2F, E2 factors; ETV, FGF-regulated TFs; FOX, forkhead box; HOX, homeodomain; IRF, interferon-regulatory factor; KLF, Krüppel-like TF’s; LHX, LIM homeobox; NKX, NKX homeodomain box; NR, nuclear receptor TF’s; PAX, paired homeobox; POU, Pit-Oct-Unc TF’s; SP, SRY-related HMG box; SP, Sp; TBX, T-box; TCF, TCF/LEF; ZBTB, ZBTB family; ZNF, zinc finger TF’s; ZSCAN, zinc finger and scan domain TF’s.

Figure 4: Histogram showing TF gene count as a function of the measured level of EZH2 protein bound at the promoter. Analysis was carried out with all 246 tissue targeted database TFs with EZH2 bound at the promoter. Note that there are preferred levels of EZH2 protein bound. J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Of the 20 families, the greatest number (16) contain both broadly expressed and tissue targeted members. The remaining four families have members with only broadly expressed (ATF and CREB) and only tissue targeted (NKX and PAX) genes. Members of the largest family (ZNF; 109 genes) are predominantly broadly expressed genes (101 broadly expressed vs. 8 tissue targeted genes). Forkhead (FOX) and homeobox (HOX) families have the highest number of tissue targeted genes (31 and 37, respectively; figure 5). The results support the conclusion that most human TF families contain both broadly expressed and tissue targeted member genes. Volume 7 • Issue 1 • 100034

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A similar analysis was performed with TFs grouped according to the structural motif found in the TF protein. Eight prominent structural motifs found among TF proteins were identified and the number of broadly expressed and tissue targeted TFs was noted in each one (Figure 6). The results resembled those observed in family groups. Of the eight structural groups, both broadly expressed, and tissue targeted TF were found to be present in all but one (ZNF-BTB; figure 6). Homeobox were found to have the highest number of tissue targeted TFs and ZNF the largest number of broadly expressed.

biology [15-18]. For a gene that might benefit the fitness of the human genome, for instance, if the gene can be broadly expressed then its pathway to incorporation into the genome is simpler than if tissue specific expression is required. In the case of tissue specific expression, the gene needs to enter the genome and be expressed, but further evolutionary steps are required to ensure that the gene is not expressed in off-target tissues. In view of the quite different nature of broadly expressed and tissue targeted genes, it is not surprising that the two gene types have different mechanisms to control their expression as described here. Tissue targeted TF genes were found to have a mechanism, involving PRC2 that is less abundant in broadly expressed TF genes. In fact, it is reasonable to expect that there are other yet to be discovered mechanisms that distinguish gene expression in broadly expressed compared to tissue targeted genes.

High abundance of EZH2/PRC2 in the promoters of tissue targeted TF An unexpected outcome of the study described here was the high proportion of EZH2/PRC2 found in the promoters of tissue targeted TF. The presence of repressive signals in the promoters was expected [19], but in view of the many types of repressive signals available in the human genome, it was expected that a greater variety would be observed. Instead, of 419 tissue targeted TF examined here, 246 (59%) were found to have EZH2/PRC2 binding sites in the promoter. The high proportion suggests repression by PCR2 is particularly well suited for repression of tissue targeted TF.

Figure 6: Number of broadly expressed and tissue targeted TFs in selected groups sharing the indicated protein structural feature(s). See supplementary table 4 for all results. Note that all structure groups contain both broadly expressed and tissue targeted TF genes. Structural groups shown: bHLH, basic helix-loop-helix; bZIP, basic leucine zipper domain; ETS, erythroblast transformation specific domain; Forkhead, forkhead box; Homeobox, homeobox sequence; NR, multiple structural domains; ZNF, zinc finger domain; ZNF-BTB, zinc finger plus bric-à-brac motifs.

Discussion Experimental strategy; comparing weakly and strongly expressing gene populations The experimental strategy adopted here suggests itself as a pathway to make further progress in understanding the control of gene expression. The idea is to start with two populations of related genes that differ in their level of expression. The results of ChIP-seq experiments are then used to compare TF binding sites in the promoter regions with the goal of identifying sites that differ in the two gene populations. In principle, such a study could begin with any two populations of genes that differ in their level of expression. In practice, however, the best outcomes might be expected with genes resembling each other in features such as function, biochemical pathway, or evolutionary time the genes entered the human genome. In view of the wide range of regulatory systems that could impact a gene, the chance of identifying genes using the same control system might be best in genes that resemble each other in other respects.

Broadly expressed compared to tissue targeted genes The distinction between broadly expressed and tissue targeted genes is a fundamental one in developmental and evolutionary J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Clues about the close link between PCR2 and tissue targeted TF might arise from considering X-chromosome inactivation, a prominent focus of PRC function. PRC2 is well known for its role in suppressing all gene expression from one X chromosome in human females. Targeted to one of the two X chromosomes by an untranslated RNA (Xist), PRC complexes bind along the entire chromosome to silence the genes permanently, creating the Barr body [20-22]. Permanent repression is not observed, however, in all X inactivation situations. Temporary, reversible inactivation has been observed in mouse and in mouse ES cells [23,24]. The case for reversible inactivation therefore needs to be considered open in the case of human TF cells. PRC2 deposition to create the Barr body differs in another significant way from deposition at promoter regions. Whereas PRC complexes coat an entire X chromosome to form a Barr body, deposition sites are widely distributed in the human genome to suppress tissue targeted TF gene expression [19]. Most tissue targeted TF genes are expected to be separate sites of PRC2 deposition. This situation creates a more formidable problem for targeting complexes at TF genes than it does with an entire X chromosome. Understanding of PCR2 targeting to non-X chromosome sites is further complicated by the ability of polycomb complexes to bind not only to chromatin, but also to RNA molecules as they are being synthesizing [25].

Families of human TF Evidence that a TF can act selectively on a tissue targeted gene (e.g. EZH2) or a broadly expressed one (e.g. POLR2A) as described here raises the possibility that such selectivity might apply to TF groups as well as to individual TFs. Could all members of a TF family, for instance, be specialized to target only broadly expressed genes? Studies to address this issue generally supported the view that most TF groups contain members able to recognize broadly expressed genes and others that recognize tissue targeted ones. Little evidence Volume 7 • Issue 1 • 100034

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was observed for broadly expressed/tissue targeted specificity among either TF families or among TFs with the same protein structural motifs (Figures 5 and 6; Supplementary Table 4). Despite the lack of overall specificity observed, some examples of selectivity were noted. For instance, zinc finger TFs exhibited a strong preference for broadly expressed TF genes while homeodomain TFs showed a preference for tissue targeted genes (Figure 6). I suggest that future studies of gene regulatory control might benefit from a focus on TF groups such as NKX and PAX in which all members have tissue targeted expression. It is expected that the promoter regions of these genes will have binding sites for both activating elements able to activate genes in the targeted tissue and repressive elements for non-targeted tissues. Examination of the promoters in such genes might facilitate the identification of the features that influence gene expression.

Data Availability All data employed in this study are contained in supplementary tables 1-4 from which it can be freely downloaded.

Acknowledgement I gratefully acknowledge Karsten Siller for advice on computational aspects of this project. This manuscript was submitted as a preprint in the link https://biorxiv.org/content/10.1101/2021.08.04.4550 52v1 [26]. EZH2 Binding

2

ATF2

B

POLR2A

32.4

1

ATF3

B

POLR2A + EZH2

30.1

22

ATF4

B

POLR2A

600.3

19

ATF5

B

POLR2A

24.3

1

ATF6

B

POLR2A

20.7

6

ATF6B

B

POLR2A

116.1

12

ATF7

B

POLR2A

32.8

4

ATOH1

T

EZH2

17.1

10

ATOH7

T

POLR2A + EZH2

2.9

21

BACH1

B

POLR2A

17.9

6

BACH2

B

POLR2A

4.9

8

9

BARHL1

T

EZH2

49.1

12

1

BARHL2

T

EZH2

84.0

12

9

BARX1

T

EZH2

47.0

8

11

BARX2

T

EZH2

143.4

6

14

BATF

B

POLR2A

10.3

11

BATF2

B/T

POLR2A

4.9

1

BATF3

T

EZH2

4.4

3

BBX

B

POLR2A

22.7 6.5

2

BCL11A

T

POLR2A > EZH2

14

BCL11B

T

POLR2A + EZH2

8.9

3

BCL6

B

POLR2A

208.4

17

BCL6B

B

POLR2A > EZH2

15.0 58.4

2

Chr

Gene

B/T

POLR2A/EZH2

Gene Expression

6

BCLAF1

B

POLR2A

20

ADNP

B

POLR2A

44.0

20

BHLH23

T

EZH2

0.5

18

ADNP2

B

POLR2A

13.3

3

BHLHE40

B

POLR2A

316.4

12

AEBP2

B

POLR2A

21.7

7

BHLHA15

T

POLR2A

192.4

5

8

BHLHE22

T

EZH2

14.8

8

2

AFF3

T

EZH2

8.8

5

AFF4

B

POLR2A

40.2

20

BMP2

B

EZH2 + POLR2A

13.6

7

AHR

B

POLR2A

49.6

15

BNC1

T

EZH2 + POLR2A

18.0

21

AIRE

T

EZH2

4.0

9

BNC2

T

POLR2A + EZH2

22.5

9

AKNA

B

POLR2A

43.8

5

BRD9

B

POLR2A

30.1

2.6

11

BSX

T

EZH2

0.8

11.7

14

MIDEAS

B

POLR2A

37.0

12

ALX1

T

EZH2 + POLR2A

8

2

4

8

1

ALX3

B

EZH2 > POLR2A

11

ALX4

T

EZH2

1.1

15

1

CAMTA1

B

POLR2A

38.0

23

ARX

T

POLR2A + EZH2

6.1

8

17

CAMTA2

B

POLR2A

91.0

3

ARGFX

T

neither

0.5

1

CASZ1

B

POLR2A + EZH2

54.9

6.2

6

CDC5L

B

POLR2A

30.0

POLR2A

40.8

5

CDX1

T

EZH2

86.8

4 10

19 10

ARID3A

B

POLR2A

ARID5B

B

1

ARNT

B

POLR2A

40.2

13

CDX2

T

EZH2

29.9

15

ARNT2

B

EZH2 + POLR2A

15.5

19

CEBPA

B

POLR2A + EZH2

72.5

11

ARNTL

B

POLR2A > EZH2

24.2

20

CEBPB

B

POLR2A

263.0

12

ARNTL2

B

POLR2A

4.1

19

CEBPG

B

POLR2A

35.0

6.1

2

CEBPZ

B

POLR2A

30.9

19

CIC

B

POLR2A

73.6

9

CIZ1

B

POLR2A

67.9

4

CLOCK

B

POLR2A

10.1

16

CRAMP1

B

POLR2A

29.5

9

CREB3

B

POLR2A

98.1

11

CREB3L1

B

POLR2A

31.4

23

ARX

T

EZH2

11

ASCL2

T

EZH2

3.1

11

ASCL3

T

neither

8.7

12

ASCL4

T

EZH2

0.1

1

ASH1L

B

POLR2A

23.7

12

ATF1

B

POLR2A

21.3

6

8

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

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7

CREB3L2

B

POLR2A

46.7

19

CREB3L3

T

neither

304.8

1

CREB3L4

B

POLR2A

26.5

7

CREB5

B

neither

7.5

12

CREBL2

B

POLR2A

40.8

11

CREBZF

B

POLR2A

74.0

10

CREM

B

POLR2A

23.7

19

CRX

T

neither

0.1

16

CTCF

B

POLR2A

40.3

20

CTCFL

T

POLR2A

7.5

7

CUX1

B

POLR2A

27.3

12

CUX2

T

POLR2A + EZH2

10.6

18

CXXC1

B

POLR2A

72.9

13

DACH1

B

neither

7.1

23

DACH2

T

neither

5.0

19

DBP

B

POLR2A

33.4

11

DBX1

T

EZH2

0.6

8

12

DBX2

T

EZH2

3.2

4

12

DDIT3

B

POLR2A

80.0

11

DEAF1

B

POLR2A

87.0

2

DLX1

T

EZH2

10.0

2

DLX2

T

EZH2 + POLR2A

1.5

17

DLX3

T

EZH2

65.4

9

14

17

DLX4

T

EZH2

3.0

10

7

DLX5

T

EZH2

34.7

8

7

DLX6

T

EZH2

9.7

11

1

DMBX1

T

neither

2.0

9

DMRT1

T

EZH2

45.4

12

9

DMRT2

T

EZH2

18.4

7 16

9

DMRT3

T

EZH2

14.0

9

DMRTA1

T

POLR2A

3.3

1

DMRTA2

T

EZH2

4.5

1

DMRTB1

T

neither

122.4

19

DMRTC2

T

neither

66.3

7

DMTF1

B

POLR2A

74.1

7

DNAJC2

B

POLR2A

13.8

19

DPF1

T

POLR2A > EZH2

30.6

11

DPF2

B

POLR2A

63.7

14

DPF3

T

EZH2

16.2

19

DPRX

T

neither

1.0

1

DR1

B

POLR2A

18.0

11

DRAP1

B

POLR2A

233.4

10

DRGX

T

EZH2

1.8

19

DUXA

T

neither

1.0

3

DZIP1L

B

POLR2A

8.4

20

E2F1

B

POLR2A

9.5

1

E2F2

T

POLR2A

12.8

6

E2F3

B

POLR2A

14.1

16

E2F4

B

POLR2A

8

E2F5

B

POLR2A

12

5

12

2

E2F6

B

POLR2A

12.6

12

E2F7

T

POLR2A

4.7

11

E2F8

T

POLR2A + EZH2

3.2

5

EBF1

B

POLRA2

36.2

10

EBF3

T

EZH2

5.0

7

20

EBF4

B

EZH2

71.4

8

9

EDF1

B

POLR2A

289.4

5

EGR1

B

POLR2A

402.0

10

EGR2

B

POLR2A > EZH2

11.0

2

EGR4

T

EZH2

14.8

11

EHF

T

POLR2A

147.3

13

ELF1

B

POLR2A

50.6

4

ELF2

B

POLR2A

24.9

1

ELF3

T

POLR2A

29.0

23

ELF4

B

POLR2A

25.2

11

ELF5

T

POLR2A

3.9

23

ELK1

B

POLR2A

46.2

12

ELK3

B

POLR2A

48.5

1

ELK4

B

POLR2A

15.7

9

2

EMX1

T

EZH2

18.8

19

10

EMX2

T

EZH2

20.2

12 12

2

EN1

T

EZH2

9.4

7

EN2

T

EZH2

79.3

8

3

EOMES

T

EZH2

26.9

12

2

EPAS1

B

POLR2A

204.9

19

ERF

B

POLR2A

72.8

21

ERG

B

EZH2 > POLR2A

28.1

6

ESR1

T

EZH2+POLR2A

45.6

14

ESR2

T

POLR2A + EZH2

1.1

11

ESRRA

B

POLR2A

65.6

14

ESRRB

T

EZH2

11.8

8

1

ESRRG

T

EZH2

3.7

8

23

ESX1

T

EZH2

19.8

4

11

ETS1

B

POLR2A

48.2

21

ETS2

B

POLR2A

242.3 174.6

7

ETV1

B

POLR2A

19

ETV2

T

POLR2A

1.8

1

ETV3

B

POLR2A

13.3 0.8

1

ETV3L

T

neither

17

ETV4

B

POLR2A

6.4

3

ETV5

B

POLR2A

17.1

12

ETV6

B

POLR2A

25.0

6

ETV7

B

EZH2 + POLR2A

5.0

11

PLEKHB1

B

POLR2A > EZH2

207.9

7

EVX1

T

EZH2

5.0

7

2

EVX2

T

EZH2

2.2

6

17

EZH1

B

POLR2A

100.7

7

EZH2

B

POLR2A

14.8

97.9

5

FAM170A

T

neither

36.8

8.9

2

FBXO41

T

POLR2A

60.1

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 8 of29 •

2

FEV

T

EZH2

4.5

12

11

FOXR1

6

T

neither

9.6

2

FIGLA

T

EZH2

0.8

23

FOXR2

T

neither

2.6

19

FIZ1

B

POLR2A

9.4

20

FOXS1

T

POLR2A

63.8

11

FLI1

B

EZH2 + POLR2A

18.6

21

GABPA

B

POLR2A

17.8

14

FOS

B

POLR2A

429.6

15

GABPB1

B

POLR2A

11.1

19

FOSB

B

POLR2A

20.5

23

GATA1

T

POLR2A

3.7

11

FOSL1

B

POLR2A + EZH2

12.2

3

GATA2

B

EZH2 > POLR2A

55.9

2

FOSL2

B

POLR2A

144.2

10

GATA3

T

EZH2 > POLR2A

269.4

14

FOXA1

T

POLR2A > EZH2

21.2

8

GATA4

T

EZH2

144.1

20

FOXA2

T

EZH2 > POLR2A

14.2

20

GATA5

T

EZH2

13.9

7

19

FOXA3

T

POLR2A

21.1

18

GATA6

T

EZH2

54.7

11

15

15

FOXB1

T

EZH2

3.4

8

7

GBX1

T

EZH2

15.4

13

9

FOXB2

T

EZH2

0.1

9

2

GBX2

T

EZH2

2.4

4

6

FOXC1

B

POLR2A + EZH2

63.9

6

GCM2

T

EZH2

1.4

6

16

FOXC2

T

EZH2 > POLR2A

36.0

1

GFI1

T

EZH2

9.7

4

1

FOXD2

T

EZH2

16.5

1

FOXD3

T

EZH2 > POLR2A

49.5

9

FOXD4

T

EZH2

3.4

5

9

GFI1B

T

neither

5.5

12

GLI1

B

POLR2A

5.9

1

12

GLI2

B

POLR2A

6.1

3

15.2

2

FOXD4L1

T

EZH2

1.5

12

GLI3

B

POLR2A

9

FOXD4L3

T

neither

0.2

1

GLIS1

B

EZH2

5.7

9

9

FOXD4L5

T

neither

0.1

2

GLIS2

B

EZH2

36.1

8

9

FOXD4L6

T

EZH2

0.3

1

1

GLIS3

T

POLR2A + EZH2

4.8

9

FOXE1

T

EZH2

164.5

11

1

GMEB1

B

POLR2A

7.0

1

FOXE3

T

EZH2

1.2

4

1

GON4L

B

POLR2A

15.0

16

FOXF1

B

EZH2

49.3

8

2

GRHL1

T

POLR2A

160.8

6

FOXF2

T

EZH2

130.0

12

8

GRHL2

T

POLR2A

41.4

14

FOXG1

T

EZH2

25.2

4

1

GRHL3

T

POLR2A + EZH2

141.8

8

FOXH1

T

EZH2 + POLR2A

0.8

19

GRLF1

B

POLR2A

26.8

5

FOXI1

T

neither

32.2

14

GSC

T

EZH2

10.5

10

FOXI2

T

EZH2

2.0

17

FOXJ1

T

EZH2 > POLR2A

15.7

1

12

FOXJ2

B

POLR2A

1

FOXJ3

B

POLR2A

17

FOXK2

B

POLR2A

19.6

16

FOXL1

T

EZH2

17.9

4 12

7

22

GSC2

T

EZH2

3.7

8

13

GSX1

T

EZH2

1.2

6

30.8

4

GSX2

T

EZH2

0.8

14

31.9

20

GZF1

B

POLR2A

12.7

5

HAND1

T

EZH2

122.8

16

4

HAND2

T

EZH2

86.4

10

41.2

3

FOXL2

T

EZH2

102.0

7

HBP1

B

POLR2A

12

FOXM1

B

POLR2A

15.8

23

HDX

B

POLR2A

2.9

17

FOXN1

T

POLR2A

64.3

4

HELT

T

EZH2

1.9

2

FOXN2

B

POLR2A

11.6

4

HERC5

B

POLR2A+EZH2

26.8

14

FOXN3

B

POLR2A

16.5

3

HES1

B

POLR2A

250.4 28.9

8

4

12

FOXN4

T

EZH2

23.6

1

HES2

T

EZH2 + POLR2A

13

FOXO1

B

POLR2A

66.8

1

HES3

T

EZH2

0.4

6

FOXO3

B

POLR2A

50.2

1

HES4

B

EZH2 + POLR2A

64.6

23

FOXO4

B

POLR2A

45.3

1

HES5

T

EZH2

8.8

12

1

FOXO6

B

POLR2A + EZH2

10.1

17

HES7

T

EZH2

7.0

6

HESX1

B

POLR2A

2.1

3

FOXP1

B

POLR2A

18.3

3

7

FOXP2

T

EZH2 + POLR2A

14.7

6

HEY2

B

EZH2 > POLR2A

56.3

HEYL

B

EZH2 + POLR2A

116.3

23

FOXP3

B

POLR2A

1.9

1

6

FOXP4

B

POLR2A

41.0

10

HHEX

B

EZH2

50.3

6

FOXQ1

T

EZH2 + POLR2A

36.0

17

HIC1

B

EZH2 + POLR2A

30.1

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

6

13

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 9 of29 •

22

HIC2

B

POLR2A

5.7

2

HOXD11

T

EZH2

15.5

6

14

HIF1A

B

POLR2A

82.9

2

HOXD12

T

EZH2

0.6

8

19

HIF3A

B

POLR2A

64.9

2

HOXD13

T

EZH2

34.0

11

6

HIVEP1

B

POLR2A

8.1

2

HOXD3

T

EZH2

29.2

14

6

HIVEP2

B

POLR2A

11.1

2

HOXD4

T

EZH2

36.1

8

1

HIVEP3

B

POLR2A

6.4

2

HOXD8

T

EZH2

50.5

12

19

ZNF875

B

POLR2A

25.8

2

HOXD9

T

EZH2 > POLR2A

173.5

17

HLF

B

POLR2A + EZH2

49.2

8

HSF1

B

POLR2A

108.6

1

HLX

B

EZH2

32.3

8

HMBOX1

B

POLR2A

8.2

7

6

HSF2

B

POLR2A

33.0

16

HSF4

B

EZH2 + POLR2A

108.1

4

HMX1

T

EZH2

2.3

4

17

HSF5

T

neither

64.5

10

HMX2

T

EZH2

1.6

11

20

ID1

B

POLR2A

204.6

7

10

HMX3

T

EZH2

1.4

2

ID2

B

POLR2A

126.5

12

HNF1A

T

POLR2A

8.7

1

ID3

B

POLR2A

357.9

17

HNF1B

T

EZH2 + POLR2A

90.4

1

IFI16

B

POLR2A

112.3

8

HNF4G

T

neither

24.4

7

IKZF1

T

POLR2A > EZH2

34.7

14

HOMEZ

B

POLR2A

9.7

2

IKZF2

B

POLR2A

9.0

4

HOPX

T

EZH2 + POLR2A

746.5

17

IKZF3

T

POLR2A + EZH2

36.8

7

HOXA1

T

EZH2 > POLR2A

3.6

12

IKZF4

B

POLR2A

10.8

7

HOXA10

T

EZH2 > POLR2A

70.5

10

IKZF5

B

POLR2A

25.5

7

HOXA11

T

EZH2=POLR2A

200.7

20

INSM1

T

EZH2

53.3

7

7

HOXA13

T

EZH2 + POLR2A

71.9

14

INSM2

T

EZH2

2.4

11

7

HOXA2

T

EZH2 > POLR2A

8.1

5

IRF1

B

POLR2A

42.2

7

HOXA3

T

EZH2

14.4

10

4

IRF2

B

POLR2A

28.9*

7

HOXA4

T

EZH2

44.6

3

19

IRF3

B

POLR2A

86.9 33.4

8

7

HOXA5

T

EZH2 + POLR2A

117.9

6

IRF4

T

POLR2A

7

HOXA6

T

EZH2 + POLR2A

14.8

7

IRF5

B

POLR2A

11.8

7

HOXA7

T

EZH2

3.1

9

1

IRF6

T

POLR2A

188.7

11

7

HOXA9

T

EZH2

33.6

17

HOXB1

T

EZH2

0.8

17

HOXB13

T

EZH2

118.8

17

HOXB2

B

EZH2 + POLR2A

47.2

5

IRX1

T

EZH2

36.0

17

HOXB3

T

EZH2 > POLR2A

33.2

5

IRX2

T

POLR2A + EZH2

40.2

17

HOXB4

B/T

EZH2

22.9

17

HOXB5

T

EZH2 > POLR2A

34.5

17

HOXB6

T

EZH2

40.1

17

HOXB7

T

EZH2 > POLR2A

17

HOXB8

T

EZH2 + POLR2A

17

HOXB9

T

16

3

11

IRF7

B

POLR2A

44.3

16

IRF8

B

POLR2A

21.0

14

IRF9

B

POLR2A

96.0

16

IRX3

T

EZH2

31.9

5

IRX4

T

EZH2 + POLR2A

31.6

16

IRX5

T

EZH2 > POLR2A

29.1

36.9

16

IRX6

T

EZH2 > POLR2A

13.4

37.3

5

ISL1

T

EZH2

28.0

EZH2 + POLR2A

35.4

15

ISL2

T

EZH2 + POLR2A

5.9

11

12

HOXC10

T

EZH2

61.6

7

22

ISX

T

POLR2A

24.8

12

HOXC11

T

EZH2

6.7

8

7

JAZF1

B

POLR2A

50.2

12

HOXC12

T

EZH2

5.4

13

14

JDP2

B

POLR2A

27.6

12

HOXC13

T

EZH2

12.9

8

1

JUN

B

POLR2A

333.6

12

HOXC4

T

EZH2

42.8

19

19

JUNB

B

POLR2A

788.0

12

HOXC5

T

EZH2

17.8

19

19

JUND

B

POLR2A

305.5

7

KIAA1549

B

POLR2A > EZH2

3.2

12

HOXC6

T

EZH2

37.9

19

12

HOXC8

T

EZH2

22.2

15

12

HOXC9

T

EZH2

22.3

2

HOXD1

T

EZH2 > POLR2A

9.1

2

HOXD10

T

EZH2

103.7

22

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

6

19

KLF1

T

POLR2A

1.4

8

KLF10

B

POLR2A

57.4

2

KLF11

B

POLR2A

27.1

13

KLF12

B

POLR2A

10.0

8

4

6

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 10 of29 •

15

KLF13

B

POLR2A

44.9

7

KLF14

T

EZH2

2.4

5

MIER3

B

POLR2A

1

MIXL1

T

EZH2

3

KLF15

B

EZH2 + POLR2A

2.3

54.8

11

MIZF

B

POLR2A

11.7

19

KLF16

B

1

KLF17

T

POLR2A

27.3

15

MKRN3

T

POLR2A

3.4

neither

24.5

10

MKX

T

EZH2

16.2

19

KLF2

B

POLR2A + EZH2

106.7

19

MLLT1

B

POLR2A

83.6

14

15.0

4

KLF3

B

POLR2A

39.5

9

MLLT3

B

POLR2A + EZH2

8.2

13

KLF5

B

POLR2A

77.5

17

MLX

B

POLR2A

58.5

10

KLF6

B

POLR2A

95.8

12

MLXIP

B

POLR2A

49.2

2

KLF7

B

POLR2A

18.1

7

MLXIPL

B

EZH2 + POLR2A

44.8

23

KLF8

B

POLR2A

20.3

17

MNT

B

POLR2A

21.1

9

KLF9

B

POLR2A

122.3

10

LBX1

T

EZH2

9.7

15

7

MNX1

T

EZH2

7.7

8

MSC

B

EZH2 + POLR2A

13.7

2

LBX2

T

EZH2 + POLR2A

1.3

4

MSX1

B

EZH2 + POLR2A

14.2

10

LCOR

B

neither

6.1

5

MSX2

T

EZH2

11.3

4

LCORL

B

POLR2A

2.7

1

MTF1

B

POLR2A

8.5

4

LEF1

T

EZH2

12.7

2

MXD1

B

POLR2A

19.2

9

19

LEUTX

T

neither

0.1

5

MXD3

B

POLR2A

12.3

17

LHX1

T

EZH2

55.1

19

4

MXD4

B

POLR2A

67.0

9

LHX2

T

EZH2

50.5

16

10

MXI1

B

POLR2A

59.6

9

LHX3

T

EZH2

89.7

4

6

MYB

T

POLR2A + EZH2

12.6

1

LHX4

B

EZH2

3.1

11

8

MYBL1

B

POLR2A

7.6

12

LHX5

T

EZH2

4.1

8

20

MYBL2

T

POLR2A

37.9

9

LHX6

T

EZH2

7.4

7

8

MYC

B

POLR2A

189.2

1

LHX8

T

EZH2

4.0

12

1

MYCL1

T

POLR2A > EZH2

38.4

1

LHX9

T

EZH2

4.1

18

2

MYCN

T

EZH2 > POLR2A

7.5

1

LMX1A

T

EZH2

2.4

8

12

MYF5

T

neither

2.1

9

LMX1B

T

EZH2

5.1

12

12

MYF6

T

POLR2A

255.0

1

MAEL

T

neither

174.2

3

MYNN

B

POLR2A

10.1

16

MAF

B

POLR2A + EZH2

76.4

11

MYOD1

T

EZH2

21.7

8

MAFA

T

EZH2

16.5

12

1

MYOG

T

neither

58.0

7

2

10

12

4

6

20

MAFB

T

EZH2

83.0

20

MYT1

T

EZH2

64.7

22

MAFF

B

POLR2A

71.3

2

MYT1L

T

neither

37.0

17

MAFG

B

POLR2A

30.4

19

MZF1

B

POLR2A

42.6

7

MAFK

B

POLR2A

89.6

12

NANOG

T

neither

0.2

5

MATR3

B

POLR2A

14.9

2

NCOA1

B

POLR2A

26.8

14

MAX

B

POLR2A

54.2

20

NCOA3

B

POLR2A

23.2

16

MAZ

B

POLR2A

35.6

2

NEUROD1

T

EZH2

306.4

15

MEF2A

B

POLR2A

46.4

12

NEUROD4

T

POLR2A

15.6

19

MEF2B

B

POLR2A

18.6

7

NEUROD6

T

EZH2

38.3

5

5

MEF2C

B

POLR2A

23.1

5

NEUROG1

T

EZH2

0.2

8

1

MEF2D

B

POLR2A

75.2

4

NEUROG2

T

EZH2

3.8

5

2

MEIS1

B

EZH2 + POLR2A

43.6

10

NEUROG3

T

EZH2

1.6

6

15

MEIS2

B

POLR2A > EZH2

33.7

16

NFAT5

B

POLR2A

15.2

19

MEIS3

T

EZH2 + POLR2A

40.1

18

NFATC1

B

POLR2A + EZH2

10.8

7

MEOX2

T

EZH2

54.2

16

NFATC3

B

POLR2A

13.8

14

NFATC4

B

POLR2A + EZH2

49.4

15

MESP2

B

EZH2 + POLR2A

1.0

15

MGA

B

POLR2A

10.5

1

MIER1

B

POLR2A

20.9

19

MIER2

B

POLR2A

13.6

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

4

12

NFE2

T

POLR2A

47.4

17

NFE2L1

B

POLR2A

224.8

2

NFE2L2

B

POLR2A

97.7

4

7

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 11 of29 •

7

NFE2L3

B

POLR2A

11.2

4

NR3C2

B

POLR2A + EZH2

9.6

1

NFIA

B

POLR2A

18.5

12

NR4A1

B

POLR2A

309.2

9

NFIB

B

POLR2A + EZH2

26.4

2

NR4A2

B

POLR2A > EZH2

26.0

19

NFIC

B

POLR2A

70.1

9

NR4A3

B/T

POLR2A + EZH2

10.0

9

NFIL3

B

POLR2A

125.1

9

NR5A1

T

EZH2

221.6

19

NFIX

B

EZH2 > POLR2A

82.1

1

NR5A2

T

EZH2 + POLR2A

7.8

8

4

NFKB1

B

POLR2A

24.8

9

NR6A1

B

POLR2A

3.9

10

NFKB2

B

POLR2A

44.1

7

NRF1

B

POLR2A

15.6

11

NFRKB

B

POLR2A

25.5

14

NRL

B

POLR2A + EZH2

2.1

9

NFX1

B

POLR2A

24.3

4

NSD2

B

POLR2A

11.0

4

NFXL1

B

POLR2A

8.5

21

OLIG2

T

EZH2

34.9

12

6

NFYA

B

POLR2A

23.5

6

OLIG3

T

EZH2

0.1

12

12

NFYB

B

POLR2A

50.8

15

ONECUT1

T

EZH2

5.5

8

1

NFYC

B

POLR2A

37.5

18

ONECUT2

T

EZH2

4.5

7

1

NHLH1

T

POLR2A

2.3

19

ONECUT3

T

EZH2

2.6

4

2

OSR1

T

EZH2

154.1

11

8

OSR2

T

EZH2

246.1

3

5

OTP

T

EZH2

9.4

15

1

NHLH2

T

EZH2

7.3

23

NKRF

B

POLR2A

9.8

14

NKX2-1

T

EZH2

352.6

7

8

20

NKX2-2

T

EZH2

43.8

12

2

OTX1

T

EZH2 + POLR2A

28.1

10

NKX2-3

T

EZH2

71.4

10

14

OTX2

T

EZH2

24.4

10

20

NKX2-4

T

EZH2

3.2

8

11

OVOL1

T

EZH2

43.5

10

5

NKX2-5

T

EZH2

113.7

11

20

OVOL2

T

EZH2

15.6

12

8

NKX2-6

T

EZH2

1.4

4

22

PATZ1

B

POLR2A

24.4

14

NKX2-8

T

EZH2

17.6

4

12

PAWR

B

POLR2A

26.9

14

NKX3-1

T

EZH2 > POLR2A

250.5

20

PAX1

T

EZH2

0.6

8

4

NKX3-2

T

EZH2

33.0

15

10

PAX2

T

EZH2

52.8

19 10

4

NKX6-1

T

EZH2

125.7

10

2

PAX3

T

EZH2

4.6

10

NKX6-2

T

EZH2

147.1

3

7

PAX4

T

EZH2

1.3

3

8

NKX6-3

T

EZH2

12.7

2

9

PAX5

T

EZH2

17.6

4

7

NOBOX

T

neither

0.9

2

NOTO

T

EZH2

0.2

6 12

11

PAX6

T

EZH2

36.9

15

1

PAX7

T

EZH2

1.7

16

1193.0

19

NPAS1

T

EZH2

10.8

2

PAX8

T

POLR2A > EZH2

2

NPAS2

B

POLR2A

26.6

14

PAX9

T

EZH2

81.1

11

NPAS4

B

EZH2 + POLR2A

9.1

1

PBX1

B

POLR2A

45.9

23

NR0B1

T

EZH2

42.5

6

PBX2

B

POLR2A

87.8

17

NR1D1

B

POLR2A

115.4

3

9

PBX3

B

POLR2A

37.9

3

NR1D2

B

POLR2A

33.7

19

PBX4

B

POLR2A

7.6 13.3

4

19

NR1H2

B

POLR2A

110.4

10

PCGF6

B

POLR2A

11

NR1H3

B

POLR2A

27.5

13

PDX1

T

EZH2

9.2

15

12

NR1H4

T

POLR2A

39.1

11

PGR

T

EZH2

111.0

12

3

NR1I2

T

POLR2A

42.6

12

PHB2

B

POLR2A

241.9

1

NR1I3

T

neither

98.7

20

PHF20

B

POLR2A

21.0

12

NR2C1

B

POLR2A

16.5

11

PHOX2A

T

EZH2 > POLR2A

2.7

3

NR2C2

B

POLR2A

21.3

4

PHOX2B

T

EZH2

4.1

4

6

NR2E1

T

EZH2

20.0

5

PITX1

T

EZH2

816.4

14

15

NR2E3

T

neither

0.6

4

PITX2

T

EZH2

24.5

12

5

NR2F1

B

EZH2

64.7

12

7

15

NR2F2

B

EZH2

66.3

11

19

NR2F6

B

POLR2A

5

NR3C1

B

POLR2A

13

10

PITX3

T

EZH2

9.3

21

PKNOX1

B

POLR2A

17.4

39.0

11

PKNOX2

B

POLR2A + EZH2

11.0

24.0

8

PLAG1

B/T

EZH2

4.2

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

5

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 12 of29 •

6

PLAGL1

B

POLR2A + EZH2

36.5

1

RCOR3

B

POLR2A

20

PLAGL2

B

POLR2A

12.7

2

REL

B

POLR2A

29.8 8.3

2

PLEK

B

POLR2A

17.6

11

RELA

B

POLR2A

88.4

3

POU1F1

T

neither

111.3

19

RELB

B

POLR2A

15.0

1

POU2F1

B

POLR2A

8.2

1

RERE

B

POLR2A

79.1

19

POU2F2

B

POLR2A

6.4

4

REST

B

POLR2A

13.1

11

POU2F3

T

POLR2A > EZH2

102.4

19

RFX1

B

POLR2A

22.6

1

POU3F1

T

EZH2

34.0

12

19

RFX2

B

POLR2A

19.1

6

POU3F2

T

EZH2

10.7

11

9

RFX3

B

POLR2A

8.3

2

POU3F3

T

EZH2

77.4

10

12

RFX4

T

EZH2

92.0

23

POU3F4

T

EZH2

28.9

2

1

RFX5

B

POLR2A

30.9

13

POU4F1

T

EZH2

2.1

10

6

RFX6

T

EZH2

1.8

4

POU4F2

T

EZH2

2.8

4

19

RFXANK

B

POLR2A

48.2

5

POU4F3

T

EZH2

0.1

5

23

RHOXF1

B

neither

5.3

6

POU5F1

B

POLR2A

6.3

23

RHOXF2

T

neither

5.5

12

POU6F1

B

POLR2A

24.0

1

RLF

B

POLR2A

20.8

7

POU6F2

T

EZH2

1.9

B

EZH2 + POLR2A

30.6

6

PPARD

B

POLR2A

43.5

9

RORB

T

EZH2

17.0

3

PPARG

B

POLR2A

11.0

1

RORC

T

POLR2A

47.7

4

15

RORA

12

8

12

6

PRDM1

B

POLR2A

19.6

6

RREB1

B

POLR2A

27.2

11

PRDM10

B

POLR2A

7.0

21

RUNX1

B

POLR2A

13.1

9

PRDM12

T

EZH2

0.8

15

6

RUNX2

B

POLR2A

9.9

6

PRDM13

T

EZH2

0.4

10

1

RUNX3

B

POLR2A

14.2

12

6

RXRB

B

POLR2A

74.6

1

RXRG

T

EZH2

43.1

8

16

SALL1

T

EZH2

50.8

4

8

PRDM14

T

EZH2

0.9

21

PRDM15

B

POLR2A

5.9

1

PRDM16

T

EZH2

20.5

6

1

PRDM2

B

POLR2A

26.5

14

SALL2

B

POLR2A

88.2

12

PRDM4

B

POLR2A

23.2

18

SALL3

T

EZH2

8.8

4

PRDM5

B

POLR2A

5.3

20

SALL4

T

POLR2A

11.9

5

PRDM6

T

EZH2

35.5

3

SATB1

B

EZH2 + POLR2A

24.2

16

PRDM7

T

POLR2A

4.0

2

SATB2

T

EZH2 + POLR2A

4.4

4

PRDM8

T/B

EZH2

25.7

8

SCRT1

T

EZH2

140.7

4

5

PRDM9

T

POLR2A-

6.9

20

SCRT2

T

EZH2

6.9

6

2

PREB

B

POLR2A

92.7

17

SEBOX

T

POLR2A

0.3

16

12

5

PROP1

T

EZH2

2.0

1

PROX1

T

EZH2 + POLR2A

30.5

14

PROX2

B

neither

24.7

1

PRRX1

T

EZH2

88.5

12

9

PRRX2

T

EZH2

46.7

4

2

10

PTF1A

T

EZH2

30.3

4

2

SIX3

17

RARA

B

POLR2A

42.6

14

SIX4

3

RARB

3

3

SHOX2

T

EZH2

26.1

16

SIM1

T

EZH2

5.8

14

21

SIM2

T

EZH2

40.7

10

14

SIX1

T

EZH2

30.5

10

SIX2

T

EZH2 + POLR2A

22.7

T

EZH2

52.0

B

POLR2A

5.4 41.3

B

POLR2A

12.7

19

SIX5

B

POLR2A

RARG

B

POLR2A

36.4

14

SIX6

T

EZH2

55.2

18

RAX

T

EZH2

0.3

1

SKI

B

POLR2A

104.4

19

RAX2

T

neither

1.9

3

SKIL

B

POLR2A

20.0

7

RBAK

B

POLR2A

8.8

15

SKOR1

T

EZH2

13.6

4

RBPJ

B

POLR2A

35.0

4

SMAD1

B

POLR2A

12.8

20

RBPJL

T

EZH2

666.8

18

SMAD2

B

POLR2A

6.8

9

RC3H2

B

POLR2A

14.3

15

SMAD3

B

POLR2A

55.2

14

RCOR1

B

POLR2A

28.2

18

SMAD4

B

POLR2A

32.3

11

RCOR2

T

POLR2A + EZH2

13.2

5

SMAD5

B

POLR2A

26.8

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

4

10

6

12

15

3

15

8

14

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 13 of29 •

13

SMAD9

B

EZH2 + POLR2A

16.9

17

SUZ12

B

POLR2A

20.4

20

SNAI1

B

POLR2A

16.3

1

TAL1

T/B

EZH2 > POLR2A

11.7

8

SNAI2

B

POLR2A>EZH2

98.7

9

TAL2

B

neither

0.7

16

SNAI3

B

POLR2A

4.8

7

TAX1BP1

B

POLR2A

34.3

9

SNAPC4

B

POLR2A

24.0

2

TBR1

T

EZH2

42.0

14

9

SOHLH1

T

EZH2

40.2

1

22

TBX1

T

EZH2

34.8

12

1

11

TBX10

T

POLR2A

5.2

1

TBX15

T

EZH2

77.9

9

6

TBX18

T

EZH2

46.7

7

13

SOX1

T

EZH2

5.2

22

SOX10

T

POLR2A + EZH2

0.9

2

SOX11

T

EZH2

2.3

20

SOX12

B

POLR2A

20.6

1

TBX19

B

POLR2A

17.7

1

SOX13

B

POLR2A

54.1

17

TBX2

B

EZH2 > POLR2A

130.4

7

3

SOX14

T

EZH2

0.6

7

TBX20

T

EZH2

20.7

17

SOX15

B

POLR2A

51.6

12

17

TBX21

T

EZH2 + POLR2A

18.5

8

8

SOX17

T

EZH2 > POLR2A

47.3

23

TBX22

T

EZH2

38.6

20

SOX18

B

EZH2 + POLR2A

51.7

12

TBX3

B

EZH2 > POLR2A

62.3

3

SOX2

T

EZH2 + POLR2A

68.0

17

TBX4

T

EZH2

54.1

15

13

SOX21

T

EZH2

27.1

12

TBX5

T

EZH2

71.0

8

23

SOX3

T

EZH2 + POLR2A

5.6

16

TBX6

B

POLR2A

3.8

5

SOX30

T

POLR2A-

137.0

6

TBXT

T

EZH2

1.4

6

SOX4

B

POLR2A > EZH2

26.8

15

TCF12

B

POLR2A

26.0

8

12

SOX5

B

POLR2A

6.4

20

TCF15

T

EZH2 > POLR2A

11.7

11

SOX6

B

POLR2A

8.0

6

TCF19

B

POLR2A

15.8

22

TCF20

B

neither

18.6

6

TCF21

T

EZH2

73.6

8

SOX7

B

EZH2 + POLR2A

34.2

16

SOX8

T

EZH2

124.9

17

SOX9

B

POLR2A > EZH2

33.2

2

TCF23

T

POLR2A

103.4

12

SP1

B

POLR2A

45.3

8

TCF24

T

EZH2

2.8

2

SP100

B

POLR2A

48.5

16

TCF25

B

POLR2A

75.0

2

SP110

B

POLR2A

19.7

19

TCF3

B

POLR2A

36.3

17

SP2

B

POLR2A

22.4

18

TCF4

B

POLR2A

24.0

2

SP3

B

POLR2A

36.8

5

TCF7

B

POLR2A

12.1

7

SP4

B

POLR2A

7.7

2

TCF7L1

B

POLR2A + EZH2

38.9

2

SP5

T

POLR2A + EZH2

27.7

10

TCF7L2

B

POLR2A

26.1

17

SP6

T

EZH2

22.5

8

20

TCFL5

B

POLR2A

22.7

12

SP7

T

EZH2

1.6

4

19

TEAD2

B

POLR2A

32.7

7

SP8

T

EZH2

4.9

6

6

TEAD3

B

POLR2A

108.9

3

6

SPDEF

T

POLR2A

211.8

12

TEAD4

B

POLR2A

12.6

11

SPI1

B

POLR2A

27.0

22

TEF

B

POLR2A

38.3

19

SPIB

T

POLR2A + EZH2

21.9

16

TERB1

T

POLR2A

17.4

12

SPIC

T

neither

23.3

10

TFAM

B

POLR2A

12.0

17

SREBF1

B

POLR2A

115.4

6

TFAP2A

T

EZH2 + POLR2A

63.2

22

SREBF2

B

POLR2A

90.1

6

TFAP2B

T

EZH2

17.4

6

SRF

B

POLR2A

99.7

20

TFAP2C

T

EZH2 > POLR2A

81.2

24

SRY

T

neither

4.5

6

TFAP2D

T

EZH2

0.4

8

ST18

T

neither

33.2

1

TFAP2E

B

POLR2A + EZH2

76.9

2

STAT1

B

POLR2A

45.1

16

TFAP4

B

POLR2A

18.2

12

STAT2

B

POLR2A

109.9

12

TFCP2

B

POLR2A

27.6

17

STAT3

B

POLR2A

101.4

23

TFCP2L1

B/T

POLR2A + EZH2

146.6

13

TFDP1

B

POLR2A

35.0

3

TFDP2

B

POLR2A

21.2

23

TFDP3

T

neither

9.6

2

STAT4

B

POLR2A

24.0

17

STAT5A

B

POLR2A

47.3

17

STAT5B

B

POLR2A

72.2

12

STAT6

B

POLR2A

161.8

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

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• Page 14 of29 •

23

TFE3

B

POLR2A

96.9

12

YBX3

B

POLR2A

250.4

6

TFEB

B

POLR2A

20.4

14

YY1

B

POLR2A

34.3

7

TFEC

T

neither

8.6

23

YY2

B

neither

7.3

18

TGIF1

B

POLR2A

18.3

23

ZBED1

B

POLR2A

0.1

23

TGIF2LX

T

POLR2A

12.6

22

ZBED4

B

POLR2A > EZH2

8.7

24

TGIF2LY

T

neither

5.5

20

ZBP1

T

EZH2 + POLR2A

17.6

15

THAP10

B

POLR2A

9.8

14

ZBTB1

B

POLR2A

31.1

1

THAP3

B

POLR2A

18.7

3

ZBTB11

B

POLR2A

11.7

17

THRA

B

POLR2A

48.6

18

ZBTB14

B

POLR2A

10.8

3

THRB

B

POLR2A + EZH2

15.5

11

ZBTB16

B

EZH2 > POLR2A

35.6

10

TLX1

T

EZH2

74.2

14

1

ZBTB17

B

POLR2A

33.8

2

TLX2

T

EZH2

2.5

9

1

ZBTB18

B

POLR2A

371.5

5

TLX3

T

EZH2

48.3

8

6

ZBTB2

B

POLR2A

14.9

1

TOE1

B

POLR2A

18.0

3

ZBTB20

B

POLR2A

1.4 9.1

8

TOX

T

POLR2A + EZH2

22.4

21

ZBTB21

B

POLR2A

20

TOX2

B

EZH2 + POLR2A

25.2

6

ZBTB24

B

POLR2A

7.8

16

TOX3

T

POLR2A + EZH2

11.0

19

ZBTB32

T

POLR2A

109.3

14

TOX4

B

POLR2A

44.6

23

ZBTB33

B

POLR2A

12.3

17

TP53

B

POLR2A

32.4

9

ZBTB34

B

POLR2A

11.5

3

TP63

T

POLR2A

138.9

3

ZBTB38

B

POLR2A

18.8

1

TP73

T

POLR2A

11.0

12

ZBTB39

B

POLR2A

5.1

19

TPRX1

T

neither

2.8

17

ZBTB4

B

POLR2A

76.3

6

TRERF1

B

POLR2A

10.1

1

ZBTB41

B

POLR2A

9.2

8

TRPS1

B

POLR2A

15.4

9

ZBTB43

B

POLR2A

13.3

13

TSC22D1

B

POLR2A

166.2

19

ZBTB45

B

POLR2A

14.9

23

TSC22D3

B

POLR2A

404.8

20

ZBTB46

B

EZH2 + POLR2A

16.1

7

TSC22D4

B

POLR2A

258.4

3

ZBTB47

B

POLR2A

75.9

18

TSHZ1

B

POLR2A

37.3

1

ZBTB48

B

POLR2A

29.7

20

TSHZ2

T

POLR2A

14.0

9

ZBTB5

B

POLR2A

19.4

19

TSHZ3

T

POLR2A + EZH2

67.3

19

ZBTB7A

B

POLR2A

33.5

11

TUB

B

EZH2

43.7

1

ZBTB7B

B

POLR2A

69.5

7

TWIST1

T

EZH2 > POLR2A

42.0

1

ZC3H11A

B

POLR2A

2.2

3

UBP1

B

POLR2A

54.8

2

ZC3H15

B

POLR2A

47.9

17

UBTF

B

POLR2A

43.2

8

ZC3H3

B

POLR2A

30.2

7

UNCX

T

EZH2

43.4

9

TUT7

B

POLR2A

16.1

16

UNKL

B

POLR2A

17.0

10

ZEB1

B

POLR2A

40.8

10

10

1

USF1

B

POLR2A

64.7

2

ZEB2

B

EZH2 + POLR2A

26.5

19

USF2

B

POLR2A

127.8

8

ZFAT

B

POLR2A

8.3

10

VAX1

T

EZH2

1.6

14

ZFHX2

B

POLR2A

4.2

2

VAX2

T/B

EZH2 + POLR2A

19.2

16

ZFHX3

B

EZH2 + POLR2A

26.0

12

VDR

B

POLR2A + EZH2

17.9

9

ZFP37

B

EZH2 > POLR2A

3.9

20

ZFP64

B

POLR2A

6.8

16

ZFPM1

B

POLR2A

5.9

12

10

VENTX

B/T

EZH2

6.7

17

VEZF1

B

POLR2A

42.3

4

20

VSX1

T

EZH2

20.9

8

8

ZFPM2

B

POLR2A > EZH2

8.6

14

VSX2

T

EZH2

0.7

19

23

ZFX

B

POLR2A

12.7

14

WDHD1

B

POLR2A

2.5

24

ZFY

B

POLR2A

5.1

19

WIZ

B

POLR2A > EZH2

32.0

14

ZFYVE26

B

POLR2A

10.7

11

WT1

T

EZH2

109.8

8

ZHX1

B

POLR2A

17.6

22

XBP1

B

POLR2A

286.0

12

8

ZHX2

B

EZH2 + POLR2A

40.5

1

YBX1

B

POLR2A

617.0

20

ZHX3

B

POLR2A

26.4

1

YBX2

T

POLR2A

1335.0

3

ZIC1

T

EZH2

426.0

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

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Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 15 of29 •

13

ZIC2

T

POLR2A+ EZH2+

298.1

23

ZIC3

T

EZH2

27.6

5

ZNF354C

B

POLR2A

6.3

6

1

ZNF362

B

POLR2A

46.3

3

ZIC4

T

EZH2

164.0

4

10

ZNF365

T

EZH2

36.5

13

ZIC5

T

EZH2

53.9

6

5

ZNF366

B

POLR2A

2.4

19

ZIK1

B

POLR2A + EZH2

4.6

9

ZNF367

B

POLR2A

3.2

19

ZIM3

T

neither

1.0

10

ZNF37A

B

POLR2A

9.5

19

ZKSCAN1

B

POLR2A

35.1

19

ZNF382

B

POLR2A

5.7

16

ZKSCAN2

B

POLR2A

2.8

12

ZNF384

B

POLR2A

43.6 23.5

6

ZKSCAN3

B

POLR2A

6.7

7

ZNF394

B

POLR2A

11

ZKSCAN4

B

POLR2A

8.8

7

ZNF398

B

POLR2A

11.0

7

ZKSCAN5

B

POLR2A

8.5

14

ZNF410

B

POLR2A

17.9

23

ZMAT1

B

POLR2A

35.8

19

ZNF415

B

POLR2A

9.0

3

ZMAT3

B

POLR2A

14.3

19

ZNF418

B

POLR2A

6.0

12

ZNF10

B

POLR2A

12.5

16

ZNF423

B

POLR2A

4.9

7

ZNF12

B

POLR2A

16.1

7

ZNF425

B

POLR2A

3.8

5

ZNF131

B

POLR2A

12.0

19

ZNF426

B

POLR2A

5.8

19

ZNF134

B

POLR2A

10.1

19

ZNF430

B

POLR2A

4.2

19

ZNF136

B

POLR2A

10.5

19

ZNF431

B

POLR2A

7.2

11

ZNF143

B

POLR2A

13.9

10

ZNF438

B

POLR2A

14.9

19

ZNF146

B

POLR2A

57.0

19

ZNF444

B

POLR2A

38.7

3

ZNF148

B

POLR2A

15.9

3

ZNF445

B

POLR2A

5.5

23

ZNF157

T

neither

2.6

7

ZNF467

B

POLR2A

21.0

8

ZNF16

B

POLR2A

13.3

19

ZNF480

B

POLR2A

6.0

16

ZNF174

B

POLR2A

11.3

10

ZNF488

T

POLR2A

83.6

19

ZNF175

B

POLR2A

8.2

1

ZNF496

B

POLR2A

17.9 5.9

6

ZNF184

B

POLR2A

8.0

4

ZBTB49

B

POLR2A

11

ZNF202

B

POLR2A

12.5

9

ZNF510

B

POLR2A

7.4

20

ZNF217

B

POLR2A

20.9

2

ZNF513

B

POLR2A

46.8

14

ZNF219

B

POLR2A

39.2

18

ZNF516

B

POLR2A

30.0

19

ZNF224

B

POLR2A

19.2

18

ZNF521

B

EZH2

12.3

18

ZNF24

B

POLR2A

26.2

19

ZNF536

T

POLR2A

17.1

8

ZNF251

B

POLR2A

48.1

19

ZNF551

B

POLR2A

9.7

19

ZNF256

B

POLR2A

8.3

19

ZNF568

B

POLR2A

3.5

19

ZNF260

B

POLR2A

7.8

1

ZNF593

B

POLR2A

1.3

16

ZNF276

B

POLR2A

27.4

16

ZNF598

B

POLR2A

54.0

22

ZNF280A

T

neither

1.9

19

ZNF606

B

POLR2A

7.8

1

ZNF281

B

POLR2A

11.5

5

ZNF608

B

POLR2A

11.5

7

ZNF282

B

POLR2A

29.8

15

ZNF609

B

POLR2A

19.8 34.0

6

ZNF292

B

POLR2A

14.2

5

ZNF622

B

POLR2A

19

ZNF296

B

POLR2A

9.2

19

ZNF628

B

POLR2A

8.2

5

ZNF300

B

POLR2A

8.9

2

ZNF638

B

POLR2A

47.5

19

ZNF304

B

POLR2A

9.3

12

ZNF641

B

POLR2A

13.7

16

ZNF319

B

POLR2A

12.1

1

ZPF69

B

POLR2A

3.9

10

ZNF32

B

POLR2A

86.5

1

ZNF644

B

POLR2A

13.8

6

ZNF322

B

POLR2A

4.6

19

ZNF649

B

POLR2A

6.0

19

ZNF333

B

POLR2A

15.2

17

ZNF652

B

POLR2A

15.7

20

ZNF335

B

POLR2A

21.8

19

ZNF653

B

POLR2A

9.7

20

ZNF341

B

POLR2A

6.8

9

ZNF658

B

POLR2A

2.1

19

ZNF350

B

POLR2A

9.5

1

ZNF683

T

neither

126.1

5

ZNF354A

B

POLR2A

12.4

5

ZNF354B

B

POLR2A

15.7

1

ZNF691

B

POLR2A

17.4

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

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Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 16 of29 •

19

ZNF699

B

POLR2A

2.9

14

ANKRD18B

T

EZH2 + POLR2A

8

ZNF703

B

POLR2A

73.9

170

ANKS6

B

POLR2A

8

ZNF704

B

POLR2A

12.9

165

ANP32B

B

POLR2A

8

ZNF706

B

POLR2A

44.0

91

ANXA1

B

POLR2A

15

ZNF710

B

POLR2A

15.7

147

AOPEP

B

POLR2A

23

ZNF711

B

POLR2A

19.4

4

APTX

B

POLR2A

12

ZNF740

B

POLR2A

21.3

11

AQP7

T

POLR2A

7

ZNF746

B

POLR2A

9.6

26

ARID3C

T

EZH2

17

ZNF750

T

POLR2A

204.1

126

AUH

B

POLR2A

7

ZNF777

B

POLR2A

19.6

7

B4GALT1

B

POLR2A

9

ZNF782

B

POLR2A

3.6

181

BAAT

T

POLR2A

7

ZNF786

B

POLR2A

8.3

8

BAG1

B

POLR2A

19

ZNF8

B

POLR2A

5.5

134

BICD2

B

POLR2A

16

ZNF821

B

POLR2A

11.6

53

C9orf128

B

POLR2A

19

ZNF829

B

POLR2A

2.2

33

C9orf131

T

neither

17

ZNF830

B

POLR2A

20.8

111

C9orf64

B

POLR2A

20

ZNF831

T

POLR2A

4.2

87

C9orf85

B

POLR2A

19

ZNF837

B

POLR2A

5.6

44

CA9

T

POLR2A

19

ZNF845

B

POLR2A

4.4

94

CARNMT1

B

POLR2A

19

ZNF91

B

POLR2A

7.6

179

CAVIN4

T

POLR2A

19

ZNF93

B

POLR2A

2.9

43

CCDC107

B

POLR2A

19

ZSCAN1

T

POLR2A

11.6

158

CCDC180

B

POLR2A

16

ZSCAN10

T

POLR2A

0.5

41

CD72

T

POLR2A

6

ZSCAN12

B

neither

7.4

155

CDC14B

B

POLR2A-

6

ZSCAN16

B

POLR2A

11.5

131

CENPP

B

POLR2A

15

ZSCAN2

B

POLR2A

4.6

9

CHMP5

B

POLR2A

19

ZSCAN20

B

POLR2A

2.3

57

CLTA

B

POLR2A

7

ZSCAN21

B

POLR2A

11.6

24

CNTFR

B

EZH2

19

ZSCAN22

B

POLR2A

3.6

77

CNTNAP3

B

neither

6

ZSCAN23

B

POLR2A

1.7

172

COL15A1

B

EZH2

15

ZSCAN29

B

POLR2A

12.1

167

CORO2A

T

POLR2A

19

ZSCAN4

T

neither

0.7

47

CREB3

B

POLR2A

19

ZSCAN5A

T

POLR2A

2.2

119

DAPK1

B

POLR2A

23

ZXDA

B

POLR2A

3.8

69

DCAF10

B

POLR2A

23

ZXDB

B

POLR2A

6.4

18

DCAF12

B

POLR2A

1

ZZZ3

B

POLR2A

14.6

25

DCTN3

B

POLR2A

2

DDX58

B

POLR2A

23

DNAI1

T

POLR2A

5

DNAJA1

B

POLR2A

32

DNAJB5

B

POLR2A

132

ECM2

B

neither

150

ERCC6L2

B

POLR2A

Supplementary Table 1: All database transcription factor genes with information regarding broadly vs. targeted expression, EZH2 vs POLR2A in the promoter; gene expression level and EZH2 binding in the promoter (1018 genes). B: broadly expressed gene; T tissue targeted gene; Gene Expression: transcripts per million (TPM); EZH2 binding in ChIP experiment; signal p-value

176

ERP44

B

POLR2A

Index

Gene

B/T

POLR2A/EZH2

140

FAM120A

B

POLR2A

185

ABCA1

B

POLR2A

29

FAM205A

T

EZH2

86

ABHD17B

B

POLR2A

30

FAM205C

T

neither

1

ACO1

B

POLR2A

36

FAM214B

B

POLR2A

115

AGTPBP1

B

POLR2A

22

FAM219A

B

POLR2A

90

ALDH1A1

B

POLR2A

54

FAM221B

T

POLR2A

72

ALDH1B1

B

POLR2A

75

ANKRD18A

T

POLR2A + EZH2

76

FAM240B

T

neither

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 17 of29 •

74

FAM95C

B

POLR2A

143

NUTM2F

T

neither

148

FANCC

B

POLR2A

96

OSTF1

B

POLR2A

146

FBP1

T

EZH2 + POLR2A

61

PAX5

T

EZH2

66

FBXO10

B

POLR2A

145

PCAT7

T

POLR2A

136

FGD3

B

POLR2A

97

PCSK5

B

POLR2A

106

FRMD3

B

EZH2 + POLR2A

141

PHF2

B

POLR2A

67

FRMPD1

T

EZH2

35

PIGO

B

POLR2A

187

FSD1L

B

POLR2A

180

PLPPR1

T

EZH2

169

GABBR2

T

EZH2

65

POLR1E

B

POLR2A

171

GALNT12

T

POLR2A

15

PRSS3

T

POLR2A

27

GALT

B

POLR2A

99

PRUNE2

B

POLR2A

48

GBA2

B

POLR2A

149

PTCH1

B

POLR2A

98

GCNT1

B

POLR2A

163

PTCSC2

T

EZH2

88

GDA

T

POLR2A + EZH2

142

PTPDC1

B

POLR2A

109

GKAP1

B

POLR2A

105

RASEF

T

EZH2 + POLR2A

56

GLIPR2

B

EZH2 + POLR2A

55

RECK

B

POLR2A

101

GNA14

B

EZH2

49

RGP1

B

POLR2A

102

GNAQ

B

POLR2A

112

RMI1

B

POLR2A

58

GNE

B

POLR2A

183

RNF20

B

POLR2A

117

GOLM1

B

POLR2A + EZH2

59

RNF38

B

POLR2A

63

GRHPR

B

POLR2A

127

ROR2

T

EZH2 + POLR2A

184

GRIN3A

T

EZH2

92

RORB

T

EZH2

154

HABP4

B

POLR2A

39

RUSC2

B

POLR2A

151

HSD17B3

B

neither

123

SEMA4D

B

POLR2A

129

IARS1

B

POLR2A

71

SHB

B

EZH2 + POLR2A

107

IDNK

B

POLR2A

122

SHC3

T

EZH2 + POLR2A

73

IGFBPL1

T

EZH2

42

SIT1

T

POLR2A

28

IL11RA

B

POLR2A

70

SLC25A51

B

POLR2A

177

INVS

B

POLR2A

113

SLC28A3

T

POLR2A

133

IPPK

B

POLR2A

152

SLC35D2

B

POLR2A

80

KGFLP2

B

neither

186

SLC44A1

B

POLR2A

31

KIAA1045

T

EZH2

82

SMC5

B

POLR2A

20

KIF24

B

POLR2A

6

SMU1

B

POLR2A

110

KIF27

B

POLR2A

51

SPAG8

B

POLR2A

83

KLF9

B

POLR2A

121

SPIN1

B

POLR2A

81

MAMDC2

T

POLR2A

128

SPTLC1

B

POLR2A

60

MELK

T

POLR2A

175

STX17

B

POLR2A

144

MFSD14B

B

POLR2A

13

SUGT1P1

B

POLR2A POLR2A

116

NAA35

B

POLR2A

137

SUSD3

B

161

NCBP1

B

POLR2A

125

SYK

B

EZH2

3

NDUFB6

B

POLR2A

168

TBC1D2

B

POLR2A POLR2A

10

NFX

B

POLR2A

159

TDRD7

B

138

NINJ1

B

POLR2A

40

TESK1

B

POLR2A

95

NMRK1

B

POLR2A

178

TEX10

B

POLR2A

12

NOL6

B

POLR2A

173

TGFBR1

B

POLR2A

130

NOL8

B

POLR2A

104

TLE1

B

POLR2A

50

NPR2

B

POLR2A-

103

TLE4

B

POLR2A

46

TLN1

B

POLR2A

89

TMC1

T

POLR2A

85

TMEM2

B

POLR2A

174

NR4A3

T

EZH2 +POLR2A

114

NTRK2

B

EZH2

21

NUDT2

B

POLR2A

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 18 of29 •

TMEM8B

B

POLR2A

176

C16orf82

16

45

TPM2

B

POLR2A

194

C17orf47

17

EZH2

166

TRIM14

B

POLR2A

5

C1orf94

1

POLR2A ZBTB33

52

KDM1A

164

TRMO

B

POLR2A

34

C2CD6

2

68

TRMT10B

B

POLR2A

38

C2orf78

2

none

84

TRPM3

T

EZH2

66

C5orf47

5

PCBP1

93

TRPM6

B

POLR2A

70

C5orf60

5

MNT

160

TSTD2

B

POLR2A

85

C6orf10

6

MAFK

118

TUT7

B

POLR2A

108

C9orf131

9

ATF7

19

UBAP1

B

POLR2A

107

C9orf135-AS1

9

ETS1

17

UBAP2

B

POLR2A

65

CABS1

4

CEBPB

16

UBE2R2

B

POLR2A

199

CABYR

18

PHF8

108

UBQLN1

B

POLR2A

206

CALR3

19

POLR2A

37

UNC13B

B

POLR2A

179

CASC16

16

POLR2A

124

UNQ6494

T

POLR2A

14

CATSPERE

1

POLR2A

34

VCP

B

POLR2A

59

CCDC110

4

PHF8

100

VPS13A

B

POLR2A + EZH2

204

CCDC114

19

POLR2A

139

WNK2

T

POLR2A + EZH2

228

CCDC116

22

TBL1XR1

162

XPA

B

POLR2A

13

CCDC185

1

EZH2

64

ZBTB5

B

POLR2A

20

CCDC27

1

none

62

ZCCHC7

B

POLR2A

43

CCDC36

3

POLR2A

182

ZNF189

B

POLR2A

48

CCDC37-AS1

3

ASH2L

153

ZNF367

B

POLR2A

134

CCDC38

12

POLR2A

135

ZNF484

B

POLR2A

186

CCDC42

17

POLR2A

78

ZNF658

B

POLR2A

133

CCDC62

12

POLR2A

156

ZNF782

B

POL

132

CCDC63

12

EZH2

120

CCDC7

10

POLR2A

Supplementary Table 2: Control; sample of all human genes. Sequential genes beginning with chr9 ACO1 (183 genes) Index

Gene

Chr

Prominent TF in Promoter

127

ACRV1

11

FOS

111

ACTL7A

9

ATF7

105

ACTL7B

9

CREB1

213

ACTL9

19

CREB1

16

ACTRT2

1

EZH2

55

ADAD1

4

HMBOX1

101

ADAM2

8

RAD21

64

ADAM29

4

IKZF1

4

ADAM30

1

ZBTB33

21

ADCY10

1

YY1

239

AKAP4

23

GATA1

42

ALS2CR12

2

26

APOBEC4

118

172

TERB1

16

POLR2A

125

CCDC83

11

POLR2A

135

CCER1

12

ZBTB33

114

CCIN

9

ATF2

138

CEP83-AS1

12

POLR2A

200

CETN1

18

XRCC5

80

CFAP206

6

KDM4A

201

CFAP53

18

POLR2A

90

CPA5

7

POLR2A POLR2A

54

CPEB2-AS1

4

86

CRISP2

6

ZBTB33

160

CT62

15

POLR2A

234

CYLC1

23

ATF2

35

DAW1

2

POLR2A

45

DAZL

3

NFATC1

ASH2L

53

DCAF4L1

4

SUZ12

1

IKZF1

96

DCAF4L2

8

NRF1

ASTN2-AS1

9

ATF2

67

DDX4

5

RELB

28

ATP1A4

1

FOXA1

106

DMRT1

9

EZH2

10

AXDND1

1

POLR2A

3

DMRTB1

1

NRF1

203

BOD1L2

18

ZBTB33

211

DMRTC2

19

ZBTB33

33

BOLL

2

EZH2

7

BRDT

1

ZBTB33

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

193

DNAH9

17

POLR2A

102

DNAJC5B

8

POLR2A

171

DPEP3

16

CTCF

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 19 of29 •

75

EGFLAM-AS2

5

FOS

216

NLRP11

19

POLR2A

223

EPPIN

20

SMC3

209

NLRP4

19

PKNOX1

159

EXD1

15

POLR2A

91

NME8

7

RAD21

51

F11-AS1

4

POLR2A

23

NUP210L

1

POLR2A

144

FAM186B

12

ATF2

166

NUTM1

15

IKZF1

214

FAM187B

19

POLR2A

81

OR2H1

6

EGR1

110

FAM205A

9

none

165

OR4N4

15

CTCF

77

FAM217A

6

FOS

180

OTOA

16

IKZF1

232

FAM46D

23

POLR2A

143

OVOS2

12

ASH2L

230

FAM47B

23

none

1

OXCT2

1

EZH2

231

FAM47C

23

none

87

PAPOLB

7

POLR2A

24

FAM71A

1

none

235

PASD1

23

MNT

74

FAM71B

5

ATF2

63

PDHA2

4

ATF2

154

FAM71D

14

FOS

83

PGK2

6

PKNOX1 CTCF

89

FBXO24

7

ZBTB33

141

PIWIL1

12

198

FBXW10

17

CHD7

142

PLCZ1

12

FOS

88

FKBP6

7

none

183

PMFBP1

16

IKZF1

153

FSCB

14

CREB1

113

PPP3R2

9

REST

32

FSIP2

2

TRIM28

71

PRDM9

5

RAD21

94

GALNTL5

7

TRIM28

184

PRM1

16

SRF

205

GGN

19

POLR2A

170

PRM2

16

AGO1

60

GK2

4

ZBTB33

222

PRND

20

FOS

164

GOLGA8F

15

none

41

PRR30

2

none

167

GOLGA8G

15

none

182

PRSS54

16

ATF2

163

GOLGA8S

15

CTCF

58

RBM46

4

SMC3

136

GSG1

12

POLR2A

229

RBMXL3

23

MAFK

130

H1FNT

12

none

227

RFPL3S

22

ZBTB33

76

HDGFL1

6

CTCF

140

RFX4

12

EZH2

157

HEATR4

14

POLR2A

18

RGSL1

1

CTCF

197

HEATR9

17

none

226

RIMBP3

22

none

17

HORMAD1

1

ZBTB33

146

RNF17

13

EZH2

225

HORMAD2

22

ATF7

47

ROPN1B

3

EZH2

99

KCNU1

8

PCBP2

155

RPGRIP1

14

EZH2

196

KIF2B

17

CREB1

212

RSPH6A

19

POLR2A

22

KLF17

1

ZBTB33

177

SEPT12

16

ZBTB33

137

KRT72

12

ZBTB33

95

SEPT14

7

ATF2

158

LDHAL6B

15

NRF1

37

SH2D6

2

POLR2A

126

LDHC

11

POLR2A

12

SHCBP1L

1

ZBTB7B

9

LRRC71

1

EZH2

218

SIGLECL1

19

SMC3

156

LRRC74A

14

EHMT2

56

SLC25A31

4

ZBTB33

236

MAGEB3

23

REST

69

SLC36A3

5

IKZF1

237

MAGEB4

23

EBF1

217

SLC6A16

19

TRIM22

238

MAGEC2

23

POLR2A

19

SLC9C2

1

POLR2A

192

MARCH10

17

EZH2

68

SLCO6A1

5

CEBPB

103

MCMDC2

8

POLR2A

27

SMCP

1

CTCF

185

MEIOC

17

EZH2

119

SPAG6

10

EZH2

46

MORC1

3

CTCF

92

SPAM1

7

FOS POLR2A

73

MROH2B

5

FOS

49

SPATA16

3

104

MROH5

8

POLR2A

189

SPATA22

17

SP1

188

MYCBPAP

17

KDM4A

117

SPATA31E1

9

CTCF

25

NBPF4

1

none

169

SPATA8

15

ZFX

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 20 of29 •

100

SPATC1

8

POLR2A

149

SPERT

13

TRIM22

11

2

SYCP1

1

EZH2

12

78

TCP11

6

ASH2L

12

79

TCTE1

6

EZH2

123

TDRD1

10

11

ASCL2

T

EZH2

bHLH

ASCL3

T

neither

bHLH

ASCL4

T

EZH2

bHLH

ATF1

B

POLR2A

bZIP

2

ATF2

B

POLR2A

bZIP

MNT

1

ATF3

B

POLR2A + EZH2

CREB family CREB family

11

TDRD5

1

KDM1A

22

ATF4

B

POLR2A

187

TEKT3

17

EZH2

19

ATF5

B

POLR2A

CRE element

178

TEKT5

16

POLR2A

1

ATF6

B

POLR2A

cAMP-dependent

190

TEX14

17

POLR2A

6

ATF6B

B

POLR2A

cAMP-dependent

52

TKTL2

4

MGA

12

ATF7

B

POLR2A

cAMP-dependent

ATOH1

T

EZH2

bHLH

168

TMCO5A

15

MAFK

4

131

TMPRSS12

12

ASH2L

10

ATOH7

T

POLR2A + EZH2

bHLH

109

TPD52L3

9

REST

21

BACH1

B

POLR2A

CNC-bZIP

224

TPTE

21

none

6

BACH2

B

POLR2A

CNC-bZIP

44

TRIM42

3

CHD4

9

BARHL1

T

EZH2

homeobox

62

TRIML1

4

REST

1

BARHL2

T

EZH2

homeobox

8

TSACC

1

POLR2A

9

BARX1

T

EZH2

homeobox

129

TSGA10IP

11

RBBP5

11

BARX2

T

EZH2

homeobox

36

TSPYL6

2

POLR2A

14

BATF

B

EZH2 > POLR2A

bZIP

72

TSSK1B

5

none

11

BATF2

B/T

POLR2A

bZIP

57

TTC29

4

SIN3A

1

BATF3

T

EZH2

bZIP

15

TTLL10

1

ZBTB33

2

BCL11A

T

POLR2A > EZH2

ZNF

84

TTLL2

6

EZH2

14

BCL11B

T

POLR2A + EZH2

ZNF

148

TUBA3C

13

ZBTB33

3

BCL6

B

POLR2A

ZNF

29

TUBA3E

2

ZBTB33

17

BCL6B

B

POLR2A > EZH2

ZNF

50

TUSC7

3

CREB1

6

BCLAF1

B

POLR2A

BCL2-assoc TF

202

TXNDC2

18

POLR2A

20

BHLH23

T

EZH2

128

UBQLN3

11

CREB1

3

BHLHB2

B

POLR2A

bHLH

139

USP44

12

POLR2A

7

BHLHB8

T

POLR2A

bHLH

208

WDR87

19

ASH2L

8

BHLHE22

T

EZH2

bHLH

191

ZPBP2

17

IKZF1

15

BNC1

T

EZH2 + POLR2A

ZNF

9

BNC2

T

POLR2A + EZH2

ZNF

1

CAMTA1

B

POLR2A

Calmodulin binding

17

CAMTA2

B

POLR2A

Calmodulin binding

5

CDX

T

EZH2

Supplementary Table 3: All database testis-specific genes with information about prominent transcription factors in the promoter. (204 genes) Chr

Gene

B/T

POLR2A/EZH2

Structure

20

ADNP

B

POLR2A

homeobox

5

CDX1

T

EZH2

18

ADNP2

B

POLR2A

homeobox

13

CDX2

T

EZH2

Homeobox

2

AFF3

T

EZH2

19

CEBPA

B

POLR2A + EZH2

bZIP recog CCAAT

Homeobox

5

AFF4

B

POLR2A

P-TEFB complex

20

CEBPB

B

POLR2A

bZIP

12

ALX1

T

EZH2 + POLR2A

Homeobox TAAT

19

CEBPG

B

POLR2A

bZIP

1

ALX3

B

EZH2 > POLR2A

homeobox

2

CEBPZ

B

POLR2A

bZIP

11

ALX4

T

EZH2

homeobox

9

CREB3

B

POLR2A

cAMP resp. bZIP

19

ARID3A

B/T

neither

ARID family

11

CREB3L1

B

POLR2A

cAMP resp. box B

7

CREB3L2

B

POLR2A

cAMP resp. bZIP

19

CREB3L3

T

neither

cAMP resp. bZIP

1

CREB3L4

B

POLR2A

cAMP resp.

7

CREB5

B

neither

cAMP resp. bZIP

12

CREBL2

B

POLR2A

cAMP resp. bZIP

11

CREBZF

B

POLR2A

CREB/ATF bZIP

16

CTCF

B

POLR2A

CCCTC binding ZNF

10

ARID5B

B

POLR2A

ARID family; histone demethy

1

ARNT

B

POLR2A

bHLH

15

ARNT2

B

EZH2 + POLR2A

bHLH

11

ARNTL

B

POLR2A > EZH2

bHLH

12

ARNTL2

B

POLR2A

bHLH

12

ASCL1

T

EZH2

bHLH

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 21 of29 •

20

CTCFL

T

POLR2A

CCCTC binding ZNF

7

EN2

T

EZH2

Homeobox

7

CUX1

B

POLR2A

Homeobox + CUT

19

ERF

B

POLR2A

ETS family

12

CUX2

T

POLR2A + EZH2

Homeobox + CUT

21

ERG

B

EZH2 > POLR2A

ETS family

13

DACH1

B

neither

Daschund domain

6

ESR1

T

EZH2+POLR2A

Estrogen receptor

23

DACH2

T

neither

Daschund domain

14

ESR2

T

POLR2A + EZH2

Estrogen receptor

11

DBX1

T

EZH2

Homeobox

11

ESRRA

B

POLR2A

Estrogen receptor related A

12

DBX2

T

EZH2

Homeobox

14

ESRRB

T

EZH2

Estrogen receptor related B

2

DLX1

T

EZH2

Homeobox

2

DLX2

T

EZH2 + POLR2A

Homeobox

1

ESRRG

T

EZH2

Estrogen receptor related G

17

DLX3

T

EZH2

Homeobox

ETS family (GGA(A/T)

17

DLX4

T

EZH2

Homeobox

7

DLX5

T

EZH2

Homeobox

7

DLX6

T

EZH2

Homeobox

9

DMRT1

T

EZH2-

9

DMRT2

T

EZH2

9

DMRT3

T

EZH2

9 1 1

DMRTA1 DMRTA2 DMRTB1

T T T

ZNF + DM DM

POLR2A EZH2 neither

19

DMRTC2

T

neither

19

DPF1

T

POLR2A > EZH2

Double PHD fingers

11

DPF2

B

POLR2A

ZNF-like

14

DPF3

T

EZH2

Chromatin remodeling

1 11

DR1 DRAP1

B B

POLR2A POLR2A

TBP Interacts with DR1

10

DRGX

T

EZH2

Homeobox

20

E2F1

B

POLR2A

E2F family

1

E2F2

T

POLR2A

E2F family

6

E2F3

B

POLR2A

E2F family

16

E2F4

B

POLR2A

E2F family

8

E2F5

B

POLR2A

E2F family

2

E2F6

B

POLR2A

E2F family

12 11 5 10

E2F7 E2F8 EBF1 EBF3

T T T T

POLR2A POLR2A + EZH2 EZH2 EZH2

E2F family E2F family ZNF Like EBF1

20

EBF4

B

EZH2

bHLH

5

EGR1

B

POLR2A

ZNF

10

EGR2

B

POLR2A > EZH2

ZNF

2

EGR4

T

EZH2

Similar to EGR2 (ZNF)

13 4

ELF1 ELF2

B B

POLR2A POLR2A

ETS TF ETS TF

1

ELF3

T

POLR2A

ETS TF

23

ELF4

B

POLR2A

ETS TF

11 23 12

ELF5 ELK1 ELK3

T B B

POLR2A POLR2A POLR2A

ETS TF ETS TF ETS TF

1

ELK4

B

POLR2A

ETS TF

2

EMX1

T

EZH2

Homeobox

10

EMX2

T

EZH2

Homeobox

2

EN1

T

EZH2

Homeobox

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

11

ETS1

B

POLR2A

21

ETS2

B

POLR2A

ETS family

7

ETV1

B

POLR2A

ETS family variant

19

ETV2

T

POLR2A

ETS family variant

1

ETV3

B

POLR2A

ETS family variant

1

ETV3L

T

neither

ETS family variant

17

ETV4

B

POLR2A

ETS family variant

3

ETV5

B

POLR2A

ETS family variant

12

ETV6

B

POLR2A

ETS family variant

6

ETV7

B

EZH2=POLR2A

ETS family variant

7

EVX1

T

EZH2

Homeobox

2

EVX2

T

EZH2

Homeobox

17

EZH1

B

POLR2A

H3K27 methyl transferase

7

EZH2

B

POLR2A

H3K27 methyl transferase

14

FOS

B

POLR2A

L-ZIP

19

FOSB

B

POLR2A

L-ZIP

11

FOSL1

B

POLR2A + EZH2

L-ZIP

2

FOSL2

B

POLR2A

L-ZIP

14

FOXA1

T

POLR2A > EZH2

Forkhead

20

FOXA2

T

EZH2 > POLR2A

Forkhead

19

FOXA3

T

POLR2A

Forkhead

15

FOXB1

T

EZH2

Forkhead

9

FOXB2

T

EZH2

Forkhead

6

FOXC1

B

POLR2A + EZH2

Forkhead

16

FOXC2

T

EZH2 > POLR2A

Forkhead

1

FOXD2

T

EZH2

Forkhead

1

FOXD3

T

EZH2 > POLR2A

Forkhead

9

FOXD4

T

EZH2

Forkhead

2

FOXD4L1

T

EZH2

Forkhead-like

9

FOXD4L3

T

neither

Forkhead-like

9

FOXD4L5

T

neither

Forkhead-like

9

FOXD4L6

T

EZH2

Forkhead-like

9

FOXE1

T

EZH2

Forkhead

1

FOXE3

T

EZH2

Forkhead

16

FOXF1

T

EZH2

Forkhead

6

FOXF2

T

EZH2

Forkhead

14

FOXG1

T

EZH2

Forkhead

8

FOXH1

T

EZH2 + POLR2A

Forkhead

5

FOXI1

T

neither

Forkhead

10

FOXI2

T

EZH2

Forkhead

17

FOXJ1

T

EZH2 > POLR2A

Forkhead

12

FOXJ2

B

POLR2A

Forkhead

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 22 of29 •

1

FOXJ3

B

POLR2A

Forkhead

1

HES3

T

EZH2

bHLH

17

FOXK2

B

POLR2A

Forkhead

1

HES4

B

EZH2 + POLR2A

bHLH

16

FOXL1

T

EZH2

Forkhead

1

HES5

T

EZH2

bHLH

3

FOXL2

T

EZH2

Forkhead

17

HES7

T

EZH2

bHLH

12

FOXM1

B

POLR2A

Forkhead

3

HESX1

B

POLR2A

Homeobox

17

FOXN1

T

POLR2A

Forkhead

6

HEY2

B

EZH2 > POLR2A

bHLH

2

FOXN2

B

POLR2A-

Forkhead

1

HEYL

B

EZH2 + POLR2A

bHLH

14

FOXN3

B

POLR2A

Forkhead

17

HIC1

B

EZH2 + POLR2A

ZNF-BTB

12

FOXN4

T

EZH2

Forkhead

22

HIC2

B

POLR2A

ZNF-BTB

13

FOXO1

B

POLR2A

Forkhead

14

HIF1A

B

POLR2A

bHLH

6

FOXO3

B

POLR2A

Forkhead

19

HIF3A

B

POLR2A

bHLH

23

FOXO4

B

POLR2A

Forkhead

6

HIVEP1

B

POLR2A

ZNF

1

FOXO6

B

POLR2A + EZH2

Forkhead

6

HIVEP2

B

POLR2A

ZNF

3

FOXP1

B

POLR2A

Forkhead

1

HIVEP3

B

POLR2A

ZNF

7

FOXP2

T

EZH2 + POLR2A

Forkhead

17

HLF

B

POLR2A + EZH2

bZIP

23

FOXP3

B

POLR2A

Forkhead

1

HLX

B

EZH2

Homeobox

6

FOXP4

B

POLR2A

Forkhead

4

HMX1

T

EZH2

Homeobox

6

FOXQ1

T

EZH2 + POLR2A

Forkhead

10

HMX2

T

EZH2

Homeobox

11

FOXR1

T

neither

Forkhead

10

HMX3

T

EZH2

Homeobox

23

FOXR2

T

neither

Forkhead

12

HNF1A

T

POLR2A

Homeobox-seq given

20

FOXS1

T

POLR2A

Forkhead

17

HNF1B

T

EZH2 + POLR2A

Homeobox- seq given NR subfamily

21

GABPA

B

POLR2A

ETS family

8

HNF4G

T

neither

15

GABPB1

B

POLR2A

Similar to B2

7

HOXA1

T

EZH2 > POLR2A

Homeobox

1

GABPB2

B

POLR2A

Similar to B1

7

HOXA10

T

EZH2 > POLR2A

Homeobox

23

GATA1

T

POLR2A

ZNF

7

HOXA11

T

EZH2=POLR2A

Homeobox

3

GATA2

B

EZH2 > POLR2A

ZNF

7

HOXA13

T

EZH2 + POLR2A

Homeobox

10

GATA3

T

EZH2 > POLR2A

ZNF

7

HOXA2

T

EZH2 > POLR2A

Homeobox

8

GATA4

T

EZH2

ZNF

7

HOXA3

T

EZH2

Homeobox

20

GATA5

T

EZH2

ZNF

7

HOXA4

T

EZH2

Homeobox

18

GATA6

T

EZH2

ZNF

7

HOXA5

T

EZH2 + POLR2A

Homeobox

7

GBX1

T

EZH2

Homeobox

7

HOXA6

T

EZH2 + POLR2A

Homeobox

2

GBX2

T

EZH2

Homeobox

7

HOXA7

T

EZH2

Homeobox

1

GFI1

T

EZH2

ZNF

7

HOXA9

T

EZH2

Homeobox

9

GFI1B

T

neither

ZNF

17

HOXB1

T

EZH2

Homeobox

12

GLI1

B

POLR2A

ZNF

17

HOXB13

T

EZH2

Homeobox

12

GLI2

B

POLR2A

ZNF

17

HOXB2

B

EZH2 + POLR2A

Homeobox

12

GLI3

B

POLR2A

ZNF

17

HOXB3

T

EZH2 > POLR2A

Homeobox

1

GLIS1

B

EZH2

ZNF

17

HOXB4

B/T

EZH2

Homeobox

2

GLIS2

T

EZH2

ZNF

17

HOXB5

T

EZH2 > POLR2A

Homeobox

1

GLIS3

T

POLR2A + EZH2

ZNF

17

HOXB6

T

EZH2

Homeobox

2

GRHL1

T

POLR2A

grainyhead

17

HOXB7

T

EZH2 > POLR2A

Homeobox

8

GRHL2

T

POLR2A

grainyhead

17

HOXB8

T

EZH2 + POLR2A

Homeobox

1

GRHL3

T

POLR2A + EZH2

grainyhead

17

HOXB9

T

EZH2 + POLR2A

Homeobox

14

GSC

T

EZH2

Homeobox bicoid

12

HOXC10

T

EZH2

Homeobox

22

GSC2

T

EZH2

Homeobox

12

HOXC11

T

EZH2

Homeobox

13

GSX1

T

EZH2

Homeobox-seq known

12

HOXC12

T

EZH2

Homeobox

4

GSX2

T

EZH2

Homeobox

12

HOXC13

T

EZH2

Homeobox

5

HAND1

T

EZH2

bHLH

12

HOXC4

T

EZH2

Homeobox

4

HAND2

T

EZH2

bHLH

12

HOXC5

T

EZH2

Homeobox

3

HES1

B

POLR2A

bHLH

12

HOXC6

T

EZH2

Homeobox

1

HES2

T

EZH2 + POLR2A

bHLH

12

HOXC8

T

EZH2

Homeobox

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 23 of29 •

12

HOXC9

T

EZH2

Homeobox

7

KLF14

T

EZH2

ZNF

2

HOXD1

T

EZH2 > POLR2A

Homeobox

3

KLF15

B

EZH2 + POLR2A

ZNF

2

HOXD10

T

EZH2

Homeobox

19

KLF16

B

POLR2A

ZNF

2

HOXD11

T

EZH2

Homeobox

1

KLF17

T

neither

ZNF

2

HOXD12

T

EZH2

Homeobox

19

KLF2

B

POLR2A + EZH2

ZNF

2

HOXD13

T

EZH2

Homeobox

4

KLF3

B

POLR2A

ZNF

2

HOXD3

T

EZH2

Homeobox

13

KLF5

B

POLR2A

ZNF

2

HOXD4

T

EZH2

Homeobox

10

KLF6

B

POLR2A+

ZNF

2

HOXD8

T

EZH2

Homeobox

2

KLF7

B

POLR2A

ZNF

2

HOXD9

T

EZH2 > POLR2A

Homeobox

23

KLF8

B

POLR2A

ZNF

8

HSF1

B

POLR2A

Heat shock TF

9

KLF9

B

POLR2A

ZNF binds GC box

6

HSF2

B

POLR2A-

Heat shock TF

10

LBX1

T

EZH2

Homeobox

16

HSF4

B

EZH2 + POLR2A

Heat shock TF

2

LBX2

T

EZH2 + POLR2A

Homeobox

17

HSF5

T

neither

Heat shock TF

10

LCOR

B

neither

20

ID1

B

POLR2A

bHLH

4

LCORL

B

POLR2A

2

ID2

B

POLR2A

bHLH

17

LHX1

T

EZH2

1

ID3

B

POLR2A

bHLH

9

LHX2

T

EZH2

LIM homeobox

6

ID4

B

POLR2A

bHLH

9

LHX3

T

EZH2

LIM homeobox

7

IKZF1

T

POLR2A > EZH2

ZNF

1

LHX4

B

EZH2

LIM homeobox

2

IKZF2

B

POLR2A

ZNF

12

LHX5

T

EZH2

LIM homeobox

12

IKZF4

B

POLR2A

ZNF

9

LHX6

T

EZH2

LIM homeobox

10

IKZF5

B

POLR2A

ZNF

1

LHX8

T

EZH2

LIM homeobox

LIM homeobox

20

INSM1

T

EZH2

ZNF

1

LHX9

T

EZH2

LIM homeobox

14

INSM2

T

EZH2

ZNF

1

LMX1A

T

EZH2

LIM homeobox LIM homeobox

5

IRF1

B

POLR2A

HTH (W)5 repeat

9

LMX1B

T

EZH2

4

IRF2

B

POLR2A

HTH

1

MAEL

T

neither

19

IRF3

B

POLR2A

HTH

16

MAF

B

POLR2A + EZH2

bZIP

6

IRF4

T

POLR2A

HTH

8

MAFA

T

EZH2

bZIP

7

IRF5

B

POLR2A

HTH

20

MAFB

T

EZH2

bZIP

1

IRF6

T

POLR2A

HTH

22

MAFF

B

POLR2A

bZIP bZIP

11

IRF7

B

POLR2A

HTH

17

MAFG

B

POLR2A

16

IRF8

B

POLR2A

HTH

7

MAFK

B

POLR2A

bZIP

14

IRF9

B

POLR2A

HTH

5

MATR3

B

POLR2A

Not TF?

5

IRX1

T

EZH2

Homeobox

14

MAX

B

POLR2A

bHLH

5

IRX2

T

POLR2A + EZH2

Homeobox

16

MAZ

B

POLR2A

ZNF

16

IRX3

T

EZH2

Homeobox

15

MEF2A

B

POLR2A

MADS box seq known

5

IRX4

T

EZH2 + POLR2A

Homeobox

19

MEF2B

B

POLR2A

MADS box

16

IRX5

T

EZH2 > POLR2A

Homeobox

5

MEF2C

B

POLR2A

MADS box

16

IRX6

T

EZH2 > POLR2A

Homeobox

1

MEF2D

B

POLR2A

MADS box

5

ISL1

T

EZH2

LIM homeobox

2

MEIS1

B

EZH2 + POLR2A

Homeobox TALE

15

ISL2

T

EZH2 + POLR2A

LIM homeobox

15

MEIS2

B

POLR2A > EZH2

Homeobox TALE

Homeobox

Homeobox TALE

22

ISX

T

POLR2A-

19

MEIS3

T

EZH2 + POLR2A

1

JUN

B

POLR2A

1

MIER1

B

POLR2A

19

JUNB

B

POLR2A

19

MIER2

B

POLR2A

19

JUND

B

POLR2A

5

MIER3

B

POLR2A

17

KLF

B

POLR2A

ZNF

19

MLLT1

B

POLR2A

YEATS domain

19

KLF1

T

POLR2A

ZNF

9

MLLT3

B

POLR2A + EZH2

YEATS domain

8

KLF10

B

POLR2A

ZNF

17

MLX

B

POLR2A

bHLH-ZIP

2

KLF11

B

POLR2A

ZNF

12

MLXIP

B

POLR2A

bHLH-ZIP

13

KLF12

B

POLR2A

ZNF

7

MLXIPL

B

EZH2=POLR2A

bHLH-ZIP

15

KLF13

B

POLR2A

ZNF

17

MNT

B

POLR2A

bHLH-ZIP

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 24 of29 •

7

MNX1

T

EZH2

Homeobox

1

NHLH2

T

EZH2

bHLH

8

MSC

B

4

MSX1

B

EZH2 + POLR2A

bHLH

14

NKX2-1

T

EZH2

Homeobox

EZH2 + POLR2A

Homeobox

20

NKX2-2

T

EZH2

5

MSX2

Homeobox

T

EZH2

Homeobox

10

NKX2-3

T

EZH2

2

Homeobox

MXD1

B

POLR2A

bHLH-ZIP

20

NKX2-4

T

EZH2

Homeobox

5

MXD3

B

POLR2A

bHLH-ZIP

5

NKX2-5

T

EZH2

Homeobox Homeobox

4

MXD4

B

POLR2A

bHLH-ZIP

8

NKX2-6

T

EZH2

10

MXI1

B

POLR2A

bHLH-ZIP

14

NKX2-8

T

EZH2

Homeobox

6

MYB

T

POLR2A + EZH2

HTH seq known

14

NKX3-1

T

EZH2 > POLR2A

Homeobox

8

MYBL1

B

POLR2A

HTH

4

NKX3-2

T

EZH2

Homeobox

20

MYBL2

T

POLR2A

HTH

4

NKX6-1

T

EZH2

Homeobox

8

MYC

B

POLR2A

bHLH

10

NKX6-2

T

EZH2

Homeobox

1

MYCL1

T

POLR2A > EZH2

bHLH

8

NKX6-3

T

EZH2

Homeobox

2

MYCN

T

EZH2 > POLR2A

bHLH

7

NOBOX

T

neither

Homeobox

12

MYF5

T

neither

bHLH

2

NOTO

T

EZH2

Homeobox

12

MYF6

T

POLR2A

bHLH

19

NPAS1

T

EZH2

bHLH

3

MYNN

B

POLR2A

ZNF-BTB

2

NPAS2

B

POLR2A

bHLH

11

MYOD1

T

EZH2

bHLH

11

NPAS4

B

EZH2 + POLR2A

bHLH

1

MYOG

T

neither

bHLH

23

NR0B1

T

EZH2

NR family

20

MYT1

T

EZH2

ZNF

17

NR1D1

B

POLR2A

NR family

2

MYT1L

T

neither

ZNF

3

NR1D2

B

POLR2A

NR family

2

NCOA1

B

POLR2A

bHLH

19

NR1H2

B

POLR2A

NR family

20

NCOA3

B

POLR2A

bHLH

11

NR1H3

B

POLR2A

NR family NR family

2

NEUROD1

T

EZH2

bHLH

12

NR1H4

T

POLR2A

12

NEUROD4

T

POLR2A

bHLH

3

NR1I2

T

POLR2A

NR family

7

NEUROD6

T

EZH2

bHLH

1

NR1I3

T

neither

NR family

5

NEUROG1

T

EZH2

bHLH

12

NR2C1

B

POLR2A

NR family

4

NEUROG2

T

EZH2

bHLH

3

NR2C2

B

POLR2A

NR family

10

NEUROG3

T

EZH2

bHLH

16

NFAT5

B

POLR2A

18

NFATC1

B

POLR2A + EZH2

16

NFATC3

B

14

NFATC4

12

6

NR2E1

T

EZH2

NR family

15

NR2E3

T

neither

NR family

5

NR2F1

B

EZH2

NR family

POLR2A

15

NR2F2

B

EZH2

NR family

B

POLR2A + EZH2

19

NR2F6

B

POLR2A

NR family

NFE2

T

POLR2A

Seq known

5

NR3C1

B

POLR2A

NR family

17

NFE2L1

B

POLR2A

4

NR3C2

B

POLR2A + EZH2

NR family

2

NFE2L2

B

POLR2A

bZIP

12

NR4A1

B

POLR2A

NR family

7

NFE2L3

B

POLR2A

bZIP

2

NR4A2

B

POLR2A > EZH2

NR family

1

NFIA

B

POLR2A

9

NR4A3

B/T

POLR2A + EZH2

NR family

9

NFIB

B

POLR2A + EZH2

9

NR5A1

T

EZH2

NR family

19

NFIC

B

POLR2A

1

NR5A2

T

EZH2 + POLR2A

NR family

9

NR6A1

B

POLR2A

NR family

7

NRF1

B

POLR2A

9

NFIL3

B

POLR2A

19

NFIX

B

EZH2>POLR2A

NFAT

NF-1 family

Seq known

4

NFKB1

B

POLR2A

14

NRL

B

POLR2A + EZH2

bZIP

10

NFKB2

B

POLR2A

21

OLIG2

T

EZH2

bHLH

11

NFRKB

B

POLR2A

6

OLIG3

T

EZH2

bHLH

9

NFX1

B

POLR2A

X box binding

15

ONECUT1

T

EZH2

Homeobox

4

NFXL1

B

POLR2A

X box binding

18

ONECUT2

T

EZH2

Homeobox

6

NFYA

B

POLR2A-

Binds CCAAT

19

ONECUT3

T

EZH2

Homeobox

12

NFYB

B

POLR2A

Binds CCAAT

2

OSR1

T

EZH2

1

NFYC

B

POLR2A

Binds CCAAT

8

OSR2

T

EZH2

1

NHLH1

T

POLR2A-

bHLH

5

OTP

T

EZH2

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Homeobox

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 25 of29 •

2

OTX1

T

EZH2 + POLR2A

Homeobox

8

PRDM14

T

EZH2

ZNF

14

OTX2

T

EZH2

Homeobox

21

PRDM15

B

POLR2A

ZNF

11

OVOL1

T

EZH2

ZNF

1

PRDM16

T

EZH2

ZNF

20

OVOL2

T

EZH2

ZNF

1

PRDM2

B

POLR2A

ZNF

22

PATZ1

B

POLR2A

POZ-AT hook-ZNF

12

PRDM4

B

POLR2A

ZNF

12

PAWR

B

POLR2A

bZIP

4

PRDM5

B

POLR2A

ZNF

20

PAX1

T

EZH2

Paired box

5

PRDM6

T

EZH2

PR domain

10

PAX2

T

EZH2

Paired box

16

PRDM7

T

POLR2A

ZNF

2

PAX3

T

EZH2

Paired box

4

PRDM8

T/B

EZH2

ZNF

7

PAX4

T

EZH2

Paired box

5

PRDM9

T

POLR2A-

ZNF

9

PAX5

T

EZH2

Paired box

2

PREB

B

POLR2A

11

PAX6

T

EZH2

Paired box

5

PROP1

T

EZH2

Homeobox

1

PAX7

T

EZH2

Paired box

1

PROX1

T

EZH2 + POLR2A

Homeobox

2

PAX8

T

POLR2A > EZH2

Paired box

14

PROX2

B

neither

Homeobox

14

PAX9

T

EZH2

Paired box

1

PRRX1

T

EZH2

Homeobox

1

PBX1

B

POLR2A

Homeobox

9

PRRX2

T

EZH2

Homeobox

6

PBX2

B

POLR2A

Homeobox

17

RARA

B

POLR2A

NR family

9

PBX3

B

POLR2A

Homeobox

3

RARB

B

POLR2A

NR family

19

PBX4

B

POLR2A

Homeobox

12

RARG

B

POLR2A

NR family

12

PHB2

B

POLR2A

18

RAX

T

EZH2

Homeobox

20

PHF20

B

POLR2A-

PHD finger ZNF?

19

RAX2

T

neither

Homeobox

11

PHOX2A

T

EZH2 > POLR2A

Homeobox

7

RBAK

B

POLR2A

ZNF

4

PHOX2B

T

EZH2

Homeobox

4

RBPJ

B

POLR2A

Binds NOTCH

5

PITX1

T

EZH2

Paired-like homeodomain

20

RBPJL

T

EZH2

Binds NOTCH

4

PITX2

T

EZH2

Paired-like homeodomain

9

RC3H2

B

POLR2A

ZNF

10

PITX3

T

EZH2

Paired-like homeodomain

14

RCOR1

B

POLR2A

Binds REST

21

PKNOX1

B

POLR2A

Homeobox

11

RCOR2

T

POLR2A + EZH2

Binds REST

11

PKNOX2

B

POLR2A + EZH2

Homeobox

1

RCOR3

B

POLR2A

Binds REST

8

PLAG1

B/T

EZH2

ZNF

2

REL

B

POLR2A

REL homology domain

6

PLAGL1

B

POLR2A + EZH2

ZNF

11

RELA

B

POLR2A

REL homology domain

20

PLAGL2

B

POLR2A

ZNF

19

RELB

B

POLR2A

REL homology domain

3

POU1F1

T

neither

POU homeobox

1

RERE

B

POLR2A

RE repeats

1

POU2F1

B

POLR2A

POU homeobox

4

REST

B

POLR2A

ZNF brain

19

POU2F2

B

POLR2A

POU homeobox

19

RFX1

B

POLR2A

RFX motif winged helix

11

POU2F3

T

POLR2A > EZH2

POU homeobox

19

RFX2

B

POLR2A

RFX motif

1

POU3F1

T

EZH2

POU homeobox

9

RFX3

B

POLR2A

RFX motif

6

POU3F2

T

EZH2

POU homeobox

12

RFX4

T

EZH2

RFX motif

2

POU3F3

T

EZH2

POU homeobox

1

RFX5

B

POLR2A

RFX motif

23

POU3F4

T

EZH2

POU homeobox

6

RFX6

T

EZH2

RFX motif

13

POU4F1

T

EZH2

POU homeobox

19

RFXANK

B

POLR2A

4

POU4F2

T

EZH2

POU homeobox

23

RHOXF1

B

neither

5

POU4F3

T

EZH2

POU homeobox

23

RHOXF2

T

neither

Homeobox

6

POU5F1

B

POLR2A

POU homeobox

15

RORA

B

neither

NR1 family

12

POU6F1

B

POLR2A

POU homeobox

9

RORB

T

EZH2

NR1 family

7

POU6F2

T

EZH2

POU homeobox

1

RORC

T

POLR2A

NR1 family

6

PPARD

B

POLR2A

NR family

21

RUNX1

B

POLR2A

CBF subunit TGTGGT

3

PPARG

B

POLR2A

NR family

6

RUNX2

B

POLR2A

CBF subunit

Homeobox

6

PRDM1

B

POLR2A

ZNF

1

RUNX3

B

POLR2A

CBF subunit

11

PRDM10

B

POLR2A

ZNF

6

RXRB

B

POLR2A

NR family

9

PRDM12

T

EZH2

ZNF

1

RXRG

T

EZH2

NR family

6

PRDM13

T

EZH2

ZNF

16

SALL1

T

EZH2

ZNF

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 26 of29 •

14

SALL2

T

POLR2A

ZNF

7

SP4

B

POLR2A

Sp family

18

SALL3

T

EZH2

ZNF

2

SP5

T

POLR2A + EZH2

Sp family

20

SALL4

T

POLR2A

ZNF

17

SP6

T

EZH2

Sp family

3

SATB1

B

EZH2 + POLR2A

Homeobox

12

SP7

T

EZH2

Sp family

2

SATB2

T

EZH2 + POLR2A

Homeobox

7

SP8

T

EZH2

Sp family

8

SCRT1

T

EZH2

ZNF

11

SPI1

B

POLR2A

ETS family

20

SCRT2

T

EZH2

ZNF

19

SPIB

T

POLR2A + EZH2

ETS family

6

SIM1

T

EZH2

bHLH

12

SPIC

T

neither

ETS family

21

SIM2

T

EZH2

bHLH

17

SREBF1

B

POLR2A

bHLH

14

SIX1

T

EZH2

Homeobox

22

SREBF2

B

POLR2A

bHLH

2

SIX2

T

EZH2 + POLR2A

Homeobox

6

SRF

B

POLR2A

MADS box

2

SIX3

T

EZH2

Homeobox

24

SRY

T

neither

HMG box

14

SIX4

B

POLR2A

Homeobox

2

STAT1

B

POLR2A

STAT family

19

SIX5

B

POLR2A

Homeobox

12

STAT2

B

POLR2A

STAT family

14

SIX6

T

EZH2

Homeobox

17

STAT3

B

POLR2A

STAT family

1

SKI

B

POLR2A

2

STAT4

B

POLR2A-

STAT family

3

SKIL

B

POLR2A

17

STAT5A

B

POLR2A

STAT family

4

SMAD1

B

POLR2A

SMAD family

17

STAT5B

B

POLR2A

STAT family

18

SMAD2

B

POLR2A

SMAD family

12

STAT6

B

POLR2A

STAT family

15

SMAD3

B

POLR2A

SMAD family

1

TAL1

B

EZH2

bHLH

18

SMAD4

B

POLR2A

SMAD family

9

TAL2

T

neither

bHLH

5

SMAD5

B

POLR2A

SMAD family

11

TBX10

T

POLR2A

T box TCACACCT

13

SMAD9

B

EZH2 + POLR2A

SMAD family

1

TBX15

T

EZH2

T box early embryo

20

SNAI1

B

POLR2A

ZNF

6

TBX18

T

EZH2

T box

8

SNAI2

B

POLR2A>EZH2

ZNF

1

TBX19

B

POLR2A-

T box

16

SNAI3

B

POLR2A

ZNF

17

TBX2

B

EZH2 >> POLR2A

T box

9

SNAPC4

B

POLR2A

SNAP family

7

TBX20

T

EZH2

T box

13

SOX1

T

EZH2

SOX family

17

TBX21

T

EZH2+POLR2A

T box

22

SOX10

T

POLR2A + EZH2

SOX family

23

TBX22

T

EZH2

T box

2

SOX11

T

EZH2

SOX family

12

TBX3

B

EZH2 > POLR2A

T box

20

SOX12

B

POLR2A

SOX family

17

TBX4

T

EZH2

T box

1

SOX13

B

POLR2A

SOX family

12

TBX5

T

EZH2

T box

3

SOX14

T

EZH2

SOX family

16

TBX6

B

POLR2A

T box

17

SOX15

B

POLR2A

SOX family

6

TBXT

T

EZH2

T box

8

SOX17

T

EZH2 > POLR2A

SOX family

15

TCF12

B

POLR2A

bHLH

20

SOX18

B

EZH2 + POLR2A

SOX family

20

TCF15

T

EZH2 > POLR2A

bHLH

3

SOX2

T

EZH2 + POLR2A

SOX family

6

TCF19

B

POLR2A

ZNF

13

SOX21

T

EZH2

SOX family

22

TCF20

B

neither

NR?

23

SOX3

T

EZH2 + POLR2A

SOX family

6

TCF21

T

EZH2

bHLH

5

SOX30

T

POLR2A-

SOX family

2

TCF23

T

POLR2A

bHLH

6

SOX4

B

POLR2A > EZH2

SOX family

8

TCF24

T

EZH2

bHLH

12

SOX5

B

POLR2A

SOX family

16

TCF25

B

POLR2A

bHLH

11

SOX6

B

POLR2A

SOX family

19

TCF3

B

POLR2A

bHLH

8

SOX7

B

EZH2 + POLR2A

SOX family

18

TCF4

B

POLR2A

bHLH

16

SOX8

T

EZH2

SOX family

5

TCF7

B

POLR2A

HMG box

17

SOX9

B

POLR2A > EZH2

SOX family

2

TCF7L1

B

POL + EZH2

HMG box

12

SP1

B

POLR2A

ZNF

10

TCF7L2

B

POLR2A

HMG box

2

SP100

B

POLR2A

? not TF?

20

TCFL5

B

POLR2A

bHLH

2

SP110

B

POLR2A

Nuclear body protein

17

SP2

B

POLR2A

Sp family

2

SP3

B

POLR2A

Sp family

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

11

TEAD1

B

POLR2A

TEA domain CATTCC[AT]

19

TEAD2

B

POLR2A

TEA domain

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 27 of29 •

6

TEAD3

B

POLR2A

TEA domain

1

YBX2

B

POLR2A

Y-box

12

TEAD4

B

POLR2A

TEA domain

12

YBX3

B

POLR2A

Y-box

6

TFAP2A

T

EZH2 + POLR2A

AP-2 group

14

YY1

B

POLR2A

ZNF

6

TFAP2B

T

EZH2

AP-2 group

23

YY2

B

neither

ZNF

20

TFAP2C

T

EZH2>POLR2A

AP-2 group

23

ZBED1

B

POLR2A

ZNF

6

TFAP2D

T

EZH2

AP-2 group

22

ZBED4

B

POLR2A > EZH2

ZNF

1

TFAP2E

B

POLR2A + EZH2

AP-2 group

14

ZBTB1

B

POLR2A-

ZNF-BTB

bHLH

16

TFAP4

B

POLR2A

12

TFCP2

B

POLR2A

3

ZBTB11

B

POLR2A

ZNF-BTB

18

ZBTB14

B

POLR2A

ZNF-BTB

23

TFCP2L1

B/T

POLR2A/EZH2

11

ZBTB16

B

EZH2 > POLR2A

ZNF-BTB

13

TFDP1

B

POLR2A

E2F dimerization

1

ZBTB17

B

POLR2A

ZNF-BTB

3

TFDP2

B

POLR2A

E2F dimerization

1

ZBTB18

B

POLR2A

ZNF

23

TFDP3

T

neither

E2F dimerization

6

ZBTB2

B

POLR2A

ZNF-BTB

23

TFE3

B

POLR2A

bHLH

3

ZBTB20

B

POLR2A

ZNF-BTB

6

TFEB

B

POLR2A

bHLH

21

ZBTB21

B

POLR2A

ZNF

7

TFEC

T

neither

bHLH

6

ZBTB24

B

POLR2A

ZNF-BTB

18

TGIF1

B

POLR2A

Homeobox (TALE)

19

ZBTB32

T

POLR2A

ZNF-BTB

23

TGIF2LX

T

POLR2A-

Homeobox

23

ZBTB33

B

POLR2A

ZNF-BTB

24

TGIF2LY

T

neither

Homeobox

9

ZBTB34

B

POLR2A

ZNF-BTB

15

THAP10

B

POLR2A

THAP domain ZNF

3

ZBTB38

B

POLR2A-

ZNF-BTB

1

THAP3

B

POLR2A

THAP domain ZNF

12

ZBTB39

B

POLR2A

ZNF-BTB

17

THRA

B

POLR2A

NR family

17

ZBTB4

B

POLR2A

ZNF-BTB

3

THRB

B

POLR2A + EZH2

NR family

1

ZBTB41

B

POLR2A

ZNF-BTB

10

TLX1

T

EZH2

Homeobox (NK-like)

9

ZBTB43

B

POLR2A

ZNF-BTB

2

TLX2

T

EZH2

Homeobox

19

ZBTB45

B

POLR2A

ZNF-BTB

5

TLX3

T

EZH2

Homeobox

20

ZBTB46

B

EZH2 + POLR2A

ZNF-BTB

8

TOX

T

POLR2A + EZH2

HMG box

3

ZBTB47

B

POLR2A

ZNF-BTB

20

TOX2

B

EZH2 + POLR2A

HMG box

1

ZBTB48

B

POLR2A

ZNF-BTB

16

TOX3

T

POLR2A + EZH2

HMG box

9

ZBTB5

B

POLR2A

ZNF-BTB

14

TOX4

B

POLR2A

HMG box

19

ZBTB7A

B

POLR2A

ZNF-BTB

17

TP53

B

POLR2A

1

ZBTB7B

B

POLR2A

ZNF-BTB

3

TP63

T

POLR2A

1

ZC3H11A

B

POLR2A

ZNF E-box homeobox

1

TP73

T

POLR2A

2

ZC3H15

B

POLR2A

ZNF E-box homeobox

13

TSC22D1

B

POLR2A

L-ZIP TSC22 domain

8

ZC3H3

B

POLR2A

ZNF E-box homeobox

23

TSC22D3

B

POLR2A

L-ZIP TSC22 domain

10

ZEB1

B

POLR2A

ZNF + homeobox

7

TSC22D4

B

POLR2A

L-ZIP TSC22 domain

2

ZEB2

B

EZH2 + POLR2A

ZNF + homeobox

18

TSHZ1

B

POLR2A

ZNF homeobox

8

ZFAT

B

POLR2A

ZNF AT-Hook domain

20

TSHZ2

T

POLR2A

ZNF homeobox

14

ZFHX2

B

POLR2A

ZNF homeobox

19

TSHZ3

T

POLR2A = EZH2

ZNF homeobox

14

ZFHX2

B

POLR2A

ZNF

3

UBP1

B

POLR2A

16

ZFHX3

B

EZH2 + POLR2A

ZNF homeobox

17

UBTF

B

POLR2A

HMG box

9

ZFP37

B

EZH2 > POLR2A

ZNF

7

UNCX

T

EZH2

Homeobox

20

ZFP64

B

POLR2A

ZNF

16

UNKL

B

POLR2A

ZNF

16

ZFPM1

B

POLR2A

ZNF FOG

1

USF1

B

POLR2A

8

ZFPM2

B

POLR2A > EZH2

ZNF FOG

19

USF2

B

POLR2A

23

ZFX

B

POLR2A

ZNF X-linked

10

VAX1

T

EZH2

Homeobox

24

ZFY

B

POLR2A

ZNF Y linked

2

VAX2

T/B

EZH2 > POLR2A

Homeobox

14

ZFYVE26

B

POLR2A

ZNF FYVE type

20

VSX1

T

EZH2

Homeobox

8

ZHX1

B

POLR2A

ZNF + homeobox

14

VSX2

T

EZH2

Homeobox

8

ZHX2

B

EZH2 + POLR2A

ZNF + homeobox

1

YBX1

B

POLR2A

Y-box

20

ZHX3

B

POLR2A

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 28 of29 •

3

ZIC1

T

EZH2

ZNF

1

ZNF362

B

POLR2A

ZNF

13

ZIC2

T

POLR2A+ EZH2+

ZNF

10

ZNF365

T

EZH2

ZNF

23

ZIC3

T

EZH2

ZNF

5

ZNF366

B

POLR2A

ZNF

3

ZIC4

T

EZH2

ZNF

9

ZNF367

B

POLR2A

ZNF

13

ZIC5

T

EZH2

ZNF

10

ZNF37A

B

POLR2A

ZNF

19

ZKSCAN1

T

POLR2A

ZNF + KRAB +SCAN

19

ZNF382

B

POLR2A

ZNF

16

ZKSCAN2

B

POLR2A

ZNF + KRAB +SCAN

12

ZNF384

B

POLR2A

ZNF

6

ZKSCAN3

B

POLR2A

ZNF + KRAB +SCAN

7

ZNF394

B

POLR2A

ZNF

11

ZKSCAN4

B

POLR2A

ZNF + KRAB +SCAN

7

ZNF398

B

POLR2A

ZNF

7

ZKSCAN5

B

POLR2A

ZNF + KRAB +SCAN

14

ZNF410

B

POLR2A

ZNF

23

ZMAT1

B

POLR2A

ZNF Martin type

19

ZNF415

B

POLR2A

ZNF

3

ZMAT3

B

POLR2A

ZNF Martin type

19

ZNF418

B

POLR2A

ZNF

12

ZNF10

B

POLR2A

ZNF

16

ZNF423

B

POLR2A

ZNF

7

ZNF12

B

POLR2A

ZNF

7

ZNF425

B

POLR2A

ZNF

5

ZNF131

B

POLR2A

ZNF

19

ZNF426

B

POLR2A

ZNF

19

ZNF134

B

POLR2A

ZNF

19

ZNF430

B

POLR2A

ZNF

19

ZNF136

B

POLR2A

ZNF

19

ZNF431

B

POLR2A

ZNF

11

ZNF143

B

POLR2A

ZNF

10

ZNF438

B

POLR2A

ZNF

19

ZNF146

B

POLR2A

ZNF

19

ZNF444

B

POLR2A

ZNF

3

ZNF148

B

POLR2A

ZNF

3

ZNF445

B

POLR2A

ZNF

23

ZNF157

T

neither

ZNF

7

ZNF467

B

POLR2A

ZNF

8

ZNF16

B

POLR2A

ZNF

19

ZNF480

B

POLR2A

ZNF

16

ZNF174

B

POLR2A

ZNF

10

ZNF488

T

POLR2A

ZNF

19

ZNF175

B

POLR2A

ZNF

1

ZNF496

B

POLR2A

ZNF

6

ZNF184

B

POLR2A

ZNF

4

ZNF509

B

POLR2A

ZNF

11

ZNF202

B

POLR2A

ZNF

9

ZNF510

B

POLR2A

ZNF

20

ZNF217

B

POLR2A

ZNF

2

ZNF513

B

POLR2A

ZNF

14

ZNF219

B

POLR2A

ZNF

18

ZNF516

B

POLR2A

ZNF ZNF

19

ZNF224

B

POLR2A

ZNF

18

ZNF521

B

EZH2

18

ZNF24

B

POLR2A

ZNF

19

ZNF536

T

POLR2A

ZNF

8

ZNF251

B

POLR2A

ZNF

19

ZNF551

B

POLR2A

ZNF ZNF

19

ZNF256

B

POLR2A

ZNF

19

ZNF568

B

POLR2A

19

ZNF260

B

POLR2A

ZNF

1

ZNF593

B

POLR2A

ZNF

16

ZNF276

B

POLR2A

ZNF

16

ZNF598

B

POLR2A

ZNF

22

ZNF280A

T

neither

ZNF

19

ZNF606

B

POLR2A

ZNF

1

ZNF281

B

POLR2A

ZNF

5

ZNF608

B

POLR2A

ZNF

7

ZNF282

B

POLR2A

ZNF

15

ZNF609

B

POLR2A

ZNF

6

ZNF292

B

POLR2A

ZNF

5

ZNF622

B

POLR2A

ZNF

19

ZNF296

B

POLR2A

ZNF

19

ZNF628

B

POLR2A

ZNF

5

ZNF300

B

POLR2A

ZNF

2

ZNF638

B

POLR2A

ZNF

19

ZNF304

B

POLR2A

ZNF

12

ZNF641

B

POLR2A

ZNF

16

ZNF319

B

POLR2A

ZNF

1

ZNF642

B

POLR2A

ZNF

10

ZNF32

B

POLR2A

ZNF

1

ZNF644

B

POLR2A

ZNF

6

ZNF322A

B

POLR2A

ZNF

19

ZNF649

B

POLR2A

ZNF

19

ZNF333

B

POLR2A

ZNF

17

ZNF652

B

POLR2A

ZNF ZNF

20

ZNF335

B

POLR2A

ZNF

19

ZNF653

B

POLR2A

20

ZNF341

B

POLR2A

ZNF

9

ZNF658

B

POLR2A

ZNF

19

ZNF350

B

POLR2A

ZNF

1

ZNF683

T

neither

ZNF

5

ZNF354A

B

POLR2A

ZNF

1

ZNF691

B

POLR2A

ZNF

5

ZNF354B

B

POLR2A

ZNF

19

ZNF699

B

POLR2A

ZNF

5

ZNF354C

B

POLR2A

ZNF

8

ZNF703

B

POLR2A

ZNF

J Genet Genomic Sci ISSN: 2574-2485, Open Access Journal DOI: 10.24966/GGS-2485/100033

Volume 7 • Issue 1 • 100034

Citation: Brown JC (2022) Role of Polycomb Repressive Complex 2 in Regulation of Human Transcription Factor Gene Expression. J Genet Genomic Sci 7: 034.

• Page 29 of29 •

8

ZNF704

B

POLR2A

ZNF

8

ZNF706

B

POLR2A

ZNF

15

ZNF710

B

POLR2A

ZNF

23

ZNF711

B

POLR2A

ZNF

12

ZNF740

B

POLR2A

ZNF

7

ZNF746

B

POLR2A

ZNF

17

ZNF750

T

POLR2A

ZNF

7

ZNF777

B

POLR2A

ZNF

9

ZNF782

B

POLR2A

ZNF

7

ZNF786

B

POLR2A

ZNF

19

ZNF8

B

POLR2A

ZNF

16

ZNF821

B

POLR2A

ZNF

19

ZNF829

B

POLR2A

ZNF ZNF

17

ZNF830

B

POLR2A

20

ZNF831

T

POLR2A

ZNF

19

ZNF837

B

POLR2A

ZNF

19

ZNF845

B

POLR2A

ZNF

19

ZNF91

B

POLR2A

ZNF

19

ZNF93

B

POLR2A

ZNF

19

ZSCAN1

T

POLR2A

ZNF + SCAN

16

ZSCAN10

T

POLR2A

ZNF + SCAN

6

ZSCAN12

B

neither

ZNF + SCAN

6

ZSCAN16

B

POLR2A

ZNF + SCAN

15

ZSCAN2

B

POLR2A-

ZNF + SCAN

19

ZSCAN20

B

POLR2A

ZNF + SCAN

7

ZSCAN21

B

POLR2A

ZNF + SCAN

19

ZSCAN22

B

POLR2A

ZNF + SCAN

6

ZSCAN23

B

POLR2A

ZNF + SCAN

15

ZSCAN29

B

POLR2A

ZNF + SCAN

19

ZSCAN4

T

neither

ZNF + SCAN

19

ZSCAN5A

T

POLR2A

ZNF + SCAN

23

ZXDA

B

POLR2A

ZNF X-linked 10X ZNF

23

ZXDB

B

POLR2A

ZNF X-linked

Supplementary Table 4: All database human transcription factor genes with annotated protein structural elements (1020 genes).

7. Lewis EB (1978) A gene complex controlling segmentation in Drosophila. Nature 276: 565-570. 8. Cao R, Tsukada Y, Zhang Y (2005) Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing. Mol Cell 20: 845-854. 9. Cao R, Wang L, Wang H, Xia L, Bromage HE, et al. (2002) Role of histone H3 lysine 27 methylation in Polycomb-group silencing. Science 298: 1039-1043. 10. Czermin B, Melfi R, McCabe D, Seitz V, Imhof A, et al. (2002) Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites. Cell 111: 185-196. 11. Kuzmichev A, Nishioka K, Bromage HE, Tempst P, Reinberg D (2002) Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein. Genes Dev 16: 28932905. 12. Muller J, Hart CM, Francis NJ, Vargas ML, Sengupta A, et al. (2002) Histone methyltransferase activity of a Drosophila Polycomb group repressor complex. Cell 111: 197-208. 13. Vaquerizas JM, Kummerfeld SK, Teichmann SA, Luscombe NM (2009) A census of human transcription factors: function, expression and evolution. Nat Rev Genet 10: 252-263. 14. Brown JC (2019) Control of human testis-specific gene expression. PLoS One 14: 0215184. 15. Hounkpe BW, Chenou F, Lima F, Paula EVD (2021) HRT Atlas v1.0 database: redefining human and mouse housekeeping genes and candidate reference transcripts by mining massive RNA-seq datasets. Nucleic Acids Res 49: 947-55. 16. Eisenberg E, Levanon EY (2013) Human housekeeping genes, revisited. Trends in Genetics 29: 569-574. 17. Sonawane AR, Platig J, Fagny M, Kuijjer ML (2017) Understanding Tissue-Specific Gene Regulation. Cell Rep 21: 1077. 18. Liu X, Yu X, Zack DJ, Zhu H, Qian J (2008) TiGER: a databbase for tissue-specific gene expression and regulation. BMC Bioinformatics 9: 271. 19. Lee TI, Jenner RG, Boyer LA, Guenther MG (2006) Control of development regulators by Polycomb in human embryonic stem cells. Cell 125: 301-313. 20. Kohlmaier A, Savarese F, Lachner M, Martens J, Jenuwein T, et al. (2004) A chromosomal memory triggered by Xist regulates histone methylation in X inactivation. PLoS Biol 2: 171.

References

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1. Lambert SA, Jolma A, Campitelli LF, Das PK, Yin Y, et al. (2018) The Human Transcription Factors. Cell 172: 650-665.

22. Plath K, Fang J, Evans SKM, Cao R, Worringer KA, et al. (2003) Role of histone H3 lysine 27 methylation in X inactivation. Science 300: 131-135.

2. Schubeler D (2015) Function and information content of DNA methylation. Nature 517: 321-326.

23. Okamoto I, Otte AP, Allis CD, Reinberg D, Heard E (2004) Epigenetic dynamics of imprinted X inactivation during early mouse development. Science 303: 644-649.

3. Szalaj P, Plewczynski D (2018) Three-dimensional organization and dynamics of the genome. Cell Biol Toxicol 34: 381-404. 4. Cavalli G, Heard E (2019) Advances in epigenetics link genetics to the environment and disease. Nature 571: 489-99.

24. Brookes E, Santiago I, Hebenstreit D, Morris KJ, Carroll T, et al. (2012) Polycomb associates genome-wide with a specific RNA polymerase II variant, and regulates metabolic genes in ESCs. Cell Stem Cell 10: 157170.

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