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GENOMIC INSTABILITY ASSESSMENT CORE
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UNIVERSITY OF HOUSTON
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mechanisms associated with genetic instability in various experimental conditions. UN
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Texas Children’s Neurological Research Institute
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Texas Children’s Texas Hospital Heart St. Luke’s Institute Hospital
BATES ST. HMRI Communications & External Relations | RICORE-025 | PA | DH | 250 | 09.2017
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houstonmethodist.org/genomicinstabilityassessment
Houston, TX 77030
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Houston Methodist
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Administrative Support Office:713.441.0733
6670 Bertner Avenue
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Hilton Houston Plaza/ Medical Center
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Genomic Instability Assessment Core
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Houston Methodist Research Institute
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to assist researchers in identifying the underlying
Core Director: Randa El-Zein, MBChB, PhD Office: 713.441.6062 rel-zein2@houstonmethodist.org
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instruments and qualified staff with the expertise
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Texas Children’s Neurological Research Institute
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Methodist is equipped with quality laboratory
Ben Taub General Hospital
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Texas Children’s Texas Hospital Heart St. Luke’s Institute Hospital
The Genomic Instability Assessment Core at Houston
HOUSTON METHODIST RESEARCH INSTITUTE
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Baylor College of Medicine
MO
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Research Institute
E.
Mary Gibb Jones Building
M.D. Anderson Cancer Center
M.D. ANDERSON BLVD.
Houston Methodist
Marriot
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CENTROSOME AMPLIFICATIONS
Genomic Instability Assessment Core
Centrosome
Merge
CENTROSOME DISENGAGEMENT Pericentrin
ASSESSMENT OF DNA DAMAGE
Merge
Houston Methodist and the Texas Medical Center
10+
Abnormal
Our Genomic Instability Assessment Core provides
5+
-H2AX
Normal
Control
community with access to advanced chromosomal imaging and analysis. The core is staffed by
CHROMOSOME SEGREGATION DEFECTS Metaphase Misalignment
experienced professionals who provide assistance with experimental design, specimen preparation
DNA
Merge
analysis of biological samples. We will help troubleshoot and optimize experiments to ensure
Normal
and microscopy imaging techniques for data
The Core provides services for the assessment of mitotic spindle apparatus dynamics.
Misaligned
the highest quality data is acquired.
MITOTIC SPINDLE ORIENTATION 5.37
Normal
5.37
Assessment includes: • M itotic spindle orientation and spindle angle measurement Telophase Bridging
7.76
DNA
Abnormal
7.76
• Centrosome disengagement
Normal a (direct)
c (rotated)
1 cosφ
=
• A ssessment of spindle assembly and chromosome separation pathways • A ssessment of consequences of mitotic alterations on DNA damage
a c
Bridge
Z-stacks
Merge
• Centrosome amplifications
• Chromosome segregation defects
SPINDLE ANGLE MEASUREMENT (13µm-thick sections)
Our Specialized Services
• Telomere length assessment using: - RT-PCR - Q-FISH