Research Pathology Core

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Research Pathology CORE

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Texas Children’s Neurological Research Institute

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UNIVERSITY OF HOUSTON

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Texas Children’s Texas Hospital Heart St. Luke’s Institute Hospital

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6670 Bertner Avenue Houston, TX 77030

houstonmethodist.org/research

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Texas Children’s Texas Hospital Heart St. Luke’s Institute Hospital

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houstonmethodist.org/researchpathology

Houston Methodist Research Institute

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Hilton Houston Plaza/ Medical Center

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Core Director: Andreana Rivera, MD

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Research Pathology Core

6565 Fannin St., M227 Houston, TX 77030 Houston, TX 77030

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Smith Tower

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Rice Bioscience Research Collaborative

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University of Texas Health & Science

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713.441.2662 Email: alrivera@houstonmethodist.org

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DRYDEN/TMC METRORAIL STATION

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RESEARCH PATHOLOGY CORE LAB LOCATED IN ROOM F554, FONDREN BUILDING

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The Houston Methodist Research Institute Research Pathology Core assists with histology techniques such as tissue embedding, tissue block sectioning, and staining. The core handles both preclinical tissue samples and patient samples with required approvals.

Ben Taub General Hospital

Baylor College of Medicine

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HOUSTON METHODIST RESEARCH INSTITUTE

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M.D. Anderson Cancer Center

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University of Texas Health & Science

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Research Pathology Core

The Research Pathology Core assists with routine histology techniques such as embedding tissue, sectioning tissue blocks, and staining sections. Custom tissue blocks can be prepared by the core staff, including multi-tissue arrays. Immunohistochemistry services offered include antibody characterization and optimization, and immunohistochemical and immunofluorescence staining.

SERVICES

• Specimen processing and embedding

- Assist in grossing specimens

- Tissue embedding in a cassette unless otherwise indicated

Immunohistochemistry Beta amyloid

TTF-1

CyclinD1

CD34

Pituitary hormones

WT-1

DBA-44

D2-40

Villin

Fascin

Desmin

AE1/AE3

Glycophorin

Fibronectin

Calretinin

IgA

MSA

Ber-EP4

IgD

MyoD1

Cytokeratins

IgM

Myogenin

CEA

Kappa/ Lambda

SMA

GFAP Neurofilament S-100

• Microtomy

• Design and creation of tissue microarrays

Synuclein

Tau

- Thickness of sections cut can be varied

- Performed using investigator supplied tissue or tissue from approved clinical patient samples

Synaptophysin Chromogranin

A1AT AFP

• Cryostat training and technical support

BRST-1

- Provide technical support in embedding and cutting samples

BRST-2

- Cryostat can also be reserved through iLab for self-service

CA19-9

Mammoglobin

CA125

• Tissue staining (H & E, Gram, GMS, Iron, Von Kossa)

Calponin

• Immunofluorescence (antibodies provided by investigator)

CD117

• Consultation with a pathologist upon request

ER

• Provide supplies e.g. cassettes, formalin, slides, molds, and slide boxes

CD99

CDX2

PR HCG Heppar-1

• Rush service available for an additional fee

• Immunohistochemistry (antibodies provided by core or investigator)

P63

- Titrate/optimize investigational antibodies

PSA

- DAB can provide red staining and double antibody staining

Inhibin

PLAP

RCC

E-Cadherin EMA MOC31 ALK-1 BCL-2 CD1a CD3 CD4 CD5 CD8 CD10 CD20 CD21 CD22 CD30 CD38 CD43 CD45 CD56 CD57 CD68 CD79a

Pax5 Tdt Lysozyme Adenovirus CMV EBV H. Pylori HBcAg HBsAg Herpes HSV I/II HPV HHV8 Toxo VZV BK virus HMB-45 MelanA Tyrosinase Caldesmon CD31

Note: Supply of stains is subject to availability.

Vimentin Myosin fast Myosin slow MLH1 MSH2 MSH6 Calcitonin Gastrin Glucagon Insulin NSE PTH Serotonin Somatostatin Thyroglobulin VIP P16 EGFR MIB-1 Napsin A PAX2 PAX8


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