The Laboratory Report Summer 2011 by Houston Methodist Professional Publications

The

Summer 2011

Laboratory Report A Publication of the Department of Pathology and Laboratory Medicine

Dr. Wayne Chandler Joins Department as Vice-Chair of Clinical Innovation

Wayne Chandler, M.D.

r. Wayne Chandler joined the Department in April as the Vice -Chair of Clinical Innovation and the medical director of the Coagulation Laboratory. He comes to Methodist from the University of Washington in Seattle, where he was chief of service for Laboratory Medicine at Harborview Medical Center.

“The discipline of Pathology is at an important crossroad, with the new health care laws and the advent of molecular medicine,” said Dr. Chandler. “This is an opportune time to expand laboratory automation and leverage our tremendous assets to further develop our molecular diagnostics and outreach programs.” While at the University of Washington, Dr. Chandler was responsible for automating several clinical service laboratories and developing new hemostasis and thrombosis assays. He was also codirector of their reference laboratory business. “Wayne has remarkable knowledge and experience. He is an incredible addition to our Department and the hospital system,” said Dr. James Musser, chair of the Department of Pathology and Laboratory Medicine. “His leadership in laboratory automation will greatly assist us in our ongoing efforts to bring enhanced diagnostic innovation to the Department and our Methodist patients.” Dr. Chandler has also made further development of the Department’s research outreach a priority. “We do a

substantial amount of research here, which provides us with another opportunity for considerable growth,” said Dr. Chandler. “We are well-positioned to provide our expertise to investigators in the Texas Medical Center and beyond, whether as a full collaborator or a provider of novel or customized research assays.” Dr. Chandler is board-certified in clinical pathology, a member of the Academy of Clinical Laboratory Physicians and Scientists, and a fellow of the College of American Pathologists. For more information on Dr. Chandler, please visit methodisthealth.com/Chandler. 

Table of Contents

Dr. Awalt to Lead Medical Staff at Willowbrook

Foster Donation Expands Blood Center Services Methodist Pathologists Chair HSCP Spring Symposium

Of Note… Archives of Pathology and Laboratory Medicine is the most highly read journal/periodical title among U.S. practicing pathologists. The June 2011 issue featured several contributions from our esteemed department faculty! If you haven't already, please see the important editorial on third-track pathology by our department chair, Dr. James Musser, as well as a special section organized by Dr. Jae Ro, and an original research article by Dr. Gustavo de la Roza. Congratulations to our department faculty members who joined the editorial board in June: Drs. Donna Coffey, Yimin Ge, Ping Wang, Aleksandar Babic, and Andreana Rivera. The June issue can be found at archivesofpathology.org. 

Dr. Leveque Receives Prestigious Overstreet Award

Hospital Auxiliary Donates New Blood Drive Van New Diagnostic Test Offerings

Methodist Laboratory Science Students Win TACLS Bowl Competition Trainee Spotlight

6 7

New Trainees Start on July 1st Dr. Zu Leads Hematopathology Research Effort for Department

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Dr. Leveque Receives Prestigious Overstreet Award

r. Christopher Leveque, medical director of the Blood Bank and Transfusion Medicine, received the John W. Overstreet, M.D. Award on Wednesday, March 30th during the Doctors’ Day 2011 celebrations in Crain Garden. The Overstreet Award was established in 1997 by The Methodist Hospital to honor and acknowledge a physician on its medical staff who exemplifies the best of the medical profession, demonstrates respect, empathy, and caring in their interactions with patients, family, and staff, and supports the mission of the hospital. “I am honored and humbled to receive this prestigious award named for such an esteemed doctor,” said Dr. Leveque. “Methodist is a great place to practice medicine. I am truly proud to be of service to my patients and their families in such a remarkable environment.” Dr. Leveque joined The Methodist Hospital in 1990 and became medical director of the Blood Bank in 2004. He is also the director of the Blood Banking and Transfusion Medicine Fellowship Program.

“Dr. Leveque is an outstanding physician. The care he provides to our patients in the Blood Donor Center and Apheresis Clinic is second to none,” said Dr. James Musser, chair of the Department of Pathology and Laboratory Medicine. “We also appreciate his contributions to the hospital’s teaching mission as a very capable and enthusiastic mentor to our residents and Dr. Leveque in the Apheresis Unit with a grateful patient. fellows.” Dr. Leveque has subspecialty thereby reducing the demand for blood certification in transfusion medicine. He products. To this end, he is currently inreceived his medical degree from the vestigating the use of various drugs and Louisiana State University School of growth factors, including basic pancreatic Medicine in New Orleans in 1981, and trypsin inhibitors (BPTIs) and plateletcompleted a residency in pathology and a rich plasma (PRP), in surgery. fellowship in transfusion medicine at The For more information on Dr. Leveque, University of Texas Medical Branch in please visit methodisthealth.com/Leveque.  Galveston, Texas. Dr. Leveque’s research is focused on outcomes studies that assess strategies to reduce bleeding and initiate healing after injury or surgery,

Dr. Awalt to Lead Medical Staff at Willowbrook

Hazel Awalt, M.D.

r. Hazel Awalt recently became the president-elect of the medical staff at Methodist Willowbrook Hospital and will become president in 2013. She is currently medical

director of laboratory services and oversees all laboratory operations and pathology services at Methodist Willowbrook. “I look forward to working closely with the medical staff, administration, and the board during my term as president-elect and subsequent term as president of the medical staff,” said Dr. Awalt. “It is a very exciting time to be participating in the growth of our hospital and the services we offer the community in North Houston.” Elected by the active medical staff of the hospital, the president has many leadership responsibilities in the areas of medical staff governance and education, quality assurance activities, safety initiatives, and maintaining accreditation standards such as those from The Joint Commission or Det Norske Veritas. The president also serves as a liaison between the medical staff, the hospital administration, and Board of Trustees. As president, Dr. Awalt will chair the Medical Executive

Committee and serve as a member of the Board of Trustees. Dr. Awalt received her MD degree from Baylor College of Medicine in Houston, Texas and completed a pathology residency at St. Luke's Episcopal Hospital, where she served as chief resident in her final year. She then completed her surgical pathology fellowship at The University of Texas M.D. Anderson Cancer Center. She joined The Methodist Hospital Physician Organization in 2005, and received her faculty appointment at the Weill Cornell Medical College in 2007. Dr. Awalt is board certified in anatomic and clinical pathology by the American Board of Pathology. For more information on Dr. Awalt, please visit methodisthealth.com/Awalt. 

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Donation from Mr. & Mrs. Joseph Foster Expands Blood Center Services

hree new apheresis services in our Donor Center,” instruments became said Dr. James Musser, chair of the operational in the Department of Pathology and Methodist Blood Donor Center Laboratory Medicine. in May as the result of a generApheresis donation is an autoous donation from Mr. and mated process in which a donor’s Mrs. Joseph Foster. One Trima blood is drawn and centrifuged to Accel Automated Blood Colremove a specific component(s), lection System and two Fenwal then the remaining blood is reAutopheresis-C Systems were turned to the donor. This allows added to the donor apheresis for the donation of blood compoarea of the Blood Donor Center nents that are needed most, such and will allow the Center to as double red cells, platelets, or keep up with the ever-growing plasma. Collection of stem cells From left, Dr. James Musser, Mr. John Hagale, Mrs. Harriet Foster, Mr. Joe demand for blood products at from the peripheral blood can also Foster, and Dr. Christopher Leveque. the hospital. be performed using these ma“Mr. and Mrs. Foster have chines. been extremely kind. Their generous gift For more information on the Blood Transfusion Medicine Service. “These mahas allowed us to significantly increase Donor Center at The Methodist Hospital, chines have had a tremendous impact on the number of patients that can be served please visit methodistbloodcenter.com.  patient care at Methodist.” by the Methodist Blood Donor Center,” “I thank Mr. and Mrs. Foster for their said Dr. Christopher Leveque, medical extraordinarily kind gift and their leaderdirector of the Blood Bank and ship in helping us to expand patient

Methodist Pathologists Chair HSCP Spring Symposiums

he 51st Annual Spring Symposium of the Houston Society of Clinical Pathologists (HSCP) was held on April 16, 2011 at the Omni Houston Hotel. For the past three years, faculty members from the Methodist Department of Pathology and Laboratory Medicine have contributed to the academic organization of this important annual event. This year’s symposium was co-chaired by Dr. Mary Schwartz, the medical director of Anatomic Pathology at The Methodist Hospital, and Dr. Adel El-Naggar, Professor of Pathology and Head and Neck Surgery at The University of Texas M.D. Anderson Cancer Center. The theme of this year’s lectures and exhibits was current advances in the pathology and management of head and neck neoplasia. Renowned thyroid pathologist from the University of Pennsylvania, Dr. Virginia LiVolsi, presented findings from her time served on the pathology panel of the Chernobyl Tumor Bank. Her research revealed a correlation between molecular changes in thyroid

tumors and exposure to radioactive iodide in young individuals subjected to the fallout from the Chernobyl disaster. Another well-received lecture, given by Dr. William Westra of Johns Hopkins University, presented data on the changing epidemiology of oral cancer. A disease that was once mostly caused by drinking and smoking in older men from lower socio-economic backgrounds is now largely caused by the presence of human papillomavirus in younger men from a higher socio-economic level. Knowledge of this subject is rapidly emerging and new guidelines for testing, treatment, and vaccination will likely follow. Dr. Philip Cagle, the medical director of Pulmonary Pathology at Methodist, will chair the 52nd Annual Spring Symposium in 2012. It will be held on April 28 th and 29th in the main auditorium of The Methodist Hospital Research Institute, and will combine the HSCP Spring Symposium (April 28th) with the Methodist Department of Pathology and Laboratory

Dr. Mary Schwartz convenes the 51st Annual Spring Symposium for the Houston Society of Clinical Pathologists.

Medicine seminar on advances in lung cancer (April 29th). For more information on the HSCP, please visit houstonpathologists.org. 

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Hospital Auxiliary Donates New Blood Drive Van

he Methodist Hospital Auxiliary and Gift Shop has purchased a new van for the Blood Donor Center to be used for mobile blood drives. The very generous gift will allow the Center to perform larger blood drives in the community and, therefore, provide more blood and blood components for patient care at the hospital. “The van will allow us to transport more staff and equipment to and from the blood drives in community locations all over Houston,” said Dr. Christopher Leveque, medical director of the Blood Bank and Transfusion Medicine at Methodist. “We are so grateful to Ms. Sophie Mize and the auxiliary staff at the Gift Shop for their generosity.” The Supreme StarTrans Senator Series II van was purchased in May and is currently being retrofitted with an electric

lift and special railing system to secure equipment. The van is expected to be in operation for the Center by the end of August. Steeped in a long history of service excellence, The Methodist Hospital Auxiliary and the Methodist Hospital Service Corps provide many unique volunteer opportunities. The Auxiliary assists the hospital in raising funds to The Supreme StarTrans Senator Series II van was purpurchase new medical equipment chased in May and is currently being retrofitted with an electric lift and special railing system to secure equipthrough the Gift Shop, Thrift Shop, ment. and vendor sales. Members of the Service Corps support patients, famiFor more information on The Methodlies, and visitors in a variety of important ist Hospital Auxiliary, please visit methservice areas including the information odisthealth.com/auxiliary.  desks, surgical waiting rooms, doctors’ coffee bar, and book cart. For more information on the Methodist Blood Donor Center, please visit methodistbloodcenter.com.

New Diagnostic Test Offerings: Freelite™ Serum Free Light Chain Assay The FreeLite assay from The Binding Site, Ltd. is used for the screening and monitoring of multiple myeloma and other B-cell proliferative disorders. It is a light-based assay that quantifies free kappa and lambda immunoglobulin light chains in serum. The measurement of light chain concentration in serum has recently been recommended by the International Multiple Myeloma Working Group for the diagnosis, prognosis, and monitoring of patients with multiple myeloma and certain other B-cell proliferative disorders, and by the National Comprehensive Cancer Network Clinical Practice Guidelines for the initial diagnosis of multiple myeloma. This test, which was previously performed at our reference lab, was brought in-house in May. It will provide Methodist physicians faster access to laboratory results and allow more timely decisions to be made regarding patient treatment.

H&E section of lymphoma.

For more information on the assay at TMHS, please contact Dr. Ping Wang at 713-441-3294 or pwang@tmhs.org. For more information on serum free light chain assays developed by The Binding Site, please visit bindingsite.co.uk/. 

InVivoScribe Gene Clonality Assay In April, The Methodist Hospital System implemented a new immunoglobulin gene rearrangement assay from InVivoScribe Technologies, Inc. called the Gene Clonality Assay. The assay uses a PCRbased amplification strategy to detect gene rearrangements in both IgH and IgK that account for 99% of all B-cell malignancies. Because of the improved sensitivity and specificity of the BIOMED-2 primers, this test has a much lower falsenegative rate compared to previous molecular tests. Validation of a similar upgrade to the T-cell clonality assay is currently underway. For more information on the assay at TMHS, please contact Dr. Randall Olsen at 713-441-6802 or rjolsen@tmhs.org. For more information on molecular diagnostics assays developed by InVivoScribe Technologies, please visit invivoscribe.com . 

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Methodist Laboratory Science Students Win TACLS Bowl Competition

he team of four students currently enrolled in The Methodist Hospital Medical Laboratory Science (MLS) Program won first place at the Texas Association for Clinical Laboratory Science (TACLS) Student Bowl competition on April 7th as part of the Sounds of Science TACLS Annual Meeting in Austin, Texas. A Methodist team has won the competition 11 times in the past 23 years. Methodist MLS students from left, Cindy Ly, Kendra Orth, “It’s a fun competition and a great Andre’lyn Williams, and Zoya Khan. way to prepare for the certification exam,” said Tatia Feltman, program director for the Methodist MLS Program. representing our program on a state“We are so proud of our students for wide level with such acclaim.” The Meththeir accomplishment and for odist MLS Program is a twelve-month

professional course of study that is fully accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS). Upon successful completion of the program, students will qualify for the examination and certification by the Board of Certification of the American Society of Clinical Pathology (ASCP). Many of the graduates of the program go on to work at Methodist in one of our many pathology laboratories. The Methodist MLS Program has been in existence since 1947. For more information on the Methodist MLS Program, please visit methodisthealth.com/mlsprogram. 

Trainee Spotlight: Kyle Eskue, M.D. Surgical Pathology Fellow

r. Kyle Eskue was the recipient of the fourth Department of Pathology and Laboratory Medicine Trainee Leadership and Innovation Award. He received this award for his overall initiative, leadership, and commitment to excellence in patient care. Dr. Eskue was recently invited to chair the College of American Pathologists (CAP) NewsPath Editorial Board. NewsPath is an educational resource for pathologists in training and practice. Dr. Eskue has been very active with CAP for many years. He is currently a junior member of the CAP Member and Public Communications Committee, the CAP Spokespersons Network, and the CAP Advocacy Network. He has previously held several leadership positions in the CAP Residents Forum. Dr. Eskue received his M.D. degree from The University of Texas Medical Branch (UTMB) in Galveston, Texas in 2006. He completed his AP/CP residency at UTMB in 2010, and then completed a fellowship in surgical pathology at Methodist in June of this year. 

Dr. Eskue in the histology laboratory.

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New Trainees Start on July 1st

he Department welcomed 6 new residents and 8 new fellows on July 1st during a half-day trainee orientation session led by Dr. Suzanne Powell, vice-chair of education for the Department. The session followed a fourday orientation provided by the hospital’s GME office. Dr. Powell and the education manager and coordinators greeted the incoming trainees and apprised them of the various support services in the Department, including Graphics Services and the Office of Academic Development. The Department will also welcome its first Clinical Chemistry Fellow on August 1st. “We have a large department that services a multi-site hospital system, and we want to make sure our new residents and fellows transition into their new roles quickly and relatively seamlessly,” said Dr. Powell. “We are preparing the next generation of practicing pathologists and want to ensure they have an outstanding training experience.” The Department currently has 37 existing trainees, with 19 of them completing their residency or fellowship program and graduated as of June 30th. With the addition of the 22 new trainees, the Department will have a total of 41 residents and fellows for the academic year 201112. The Department of Pathology and Laboratory Medicine has a residency program, a resident research program, and 11 fellowship programs – the most training programs of any department at Methodist.

Incoming Trainees PGY1 Residents Eunice Choi, M.D. Indiana University School of Medicine Suzanne M. Crumley, M.D. The University of Iowa Carver College of Medicine

Yu Helen Zhang, M.D. AP/CP Residency – Temple University Hospital Jose Gonzalez-Berjon, M.D. AP/CP Residency – The Methodist Hospital

Rachel Donohue, M.D. University of New Mexico School of Medicine

Molecular Genetic Pathology Amanda Peterson, M.D. Surgical Pathology Fellowship – The Methodist Hospital

Jaclyn L. Jerz, M.D. The University of Texas Southwestern Medical School

Neuropathology Adriana Olar, M.D. AP Residency – Baylor College of Medicine

Nathan E. Lee, M.D. The University of Texas Medical Branch

Jennifer Ross, M.D. Forensic Pathology Fellowship – Harris County Institute of Forensic Sciences

Haijun (Steve) Zhou, M.D., Ph.D. Tongji Medical University/Peking Union Medical University Fellows Blood Bank/Transfusion Medicine Jian Chen, M.D., Ph.D. CP Residency – The Methodist Hospital Clinical Chemistry Irene Shu, Ph.D. University of Washington

Surgical Pathology Natalia Golardi, M.D. AP/CP Residency – The University of Texas Medical Branch Susan Haley, M.D. AP/CP Residency – The University of Texas Medical Branch Ekene Uzoigwe Okoye, M.D. AP/CP Residency – The University of Texas Medical School, Houston

Cytopathology Kelly Khan, M.D. Surgical Pathology Fellowship – The Methodist Hospital

Annisa Lewis, M.D. AP/CP Residency – The Methodist Hospital

Aaron Harvey, M.D. AP/CP Residency – The Methodist Hospital Adria Hartman, M.D. AP/CP Residency – University of Kentucky Hematopathology Mark Podberezin, M.D. AP/CP Residency – University of Illinois at Chicago

Byron Moore, M.D. Blood Bank/Transfusion Medicine Fellowship – The Methodist Hospital

For more information on training programs in the Department, please visit methodisthealth.com/pathologytraining. 

In Our Next Issue… Department Acquires Transmission Electron Microscope

First Trainee in Resident Research Program

Digital Pathology at Methodist

New Clinical Chemistry Fellowship

Laboratory Outreach Program Expands

… and more!

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Dr. Zu Leads Hematopathology Research Effort for Department

ith funding from the National Institutes of Health and other sources, Dr. Youli Zu is developing novel diagnostic and therapeutic methods specific for lymphomas. His research laboratory was the first to demonstrate the use of oligonucleotide aptamer probes for both in vitro diagnosis and in vivo imaging of lymphoma tumors. Dr. Zu is the principal investigator on a five-year grant from Dr. Zu at TMHRI. the National Cancer Institute (NCI) that is validating a new on a grant from the National Library of bifunctional nanomedicine to selectively Medicine that aims to develop a systems target anaplastic large cell lymphoma biology software package capable of (ALCL) tumors for in vivo imaging and identifying signal pathways and pathway specifically silence the lymphoma oncosignatures in myelodysplastic syndromes gene to induce tumor cell death. In addiand other diseases with complex phenotion, Dr. Zu is co-principal investigator types. Dr. Zu also leads the pathology (with Dr. Xiaobo Zhou, also at Methodist)

tissue core of an NCI-funded lymphoma SPORE program. Dr. Jianguo Wen, an instructor in Dr. Zu’s TMHRI laboratory, also has funding from NCI. His 2-year grant aims to use cytotoxic T-cells to develop an effective immunotherapy that targets myeloma stem cells. Dr. Wen joined the laboratory in 2005 as a postdoctoral fellow under the mentorship of then laboratory director, Dr. Chung-Che “Jeff” Chang. Dr. Zu serves as the co-director of hematopathology for The Methodist Hospital. For more information about Dr. Zu, please visit methodisthealth.com/Zu. 

RECENT PUBLICATIONS Blieden LS, Chévez-Barrios P, Yen MT. Herpes simplex vegetans presenting as an eyelid mass. Ophthal Plast Reconstr Surg. 2011 MayJun;27(3):e58-9. Bul M, Delongchamps NB, Steyerberg EW, de la Roza G, van Leeuwen PJ, Zhu X, van Vugt HA, Haas GP, Schröder FH, Roobol MJ. Updating the prostate cancer risk indicator for contemporary biopsy schemes. Can J Urol. 2011 Apr;18(2):5625-9. Cagle PT, Allen TC. Pathology of the pleura: what the pulmonologists need to know. Respirology. 2011 Apr;16(3):430-8. Cagle PT, Dacic S. Lung cancer and the future of pathology. Arch Pathol Lab Med. 2011 Mar;135(3):293-5. Carroll RK, Musser JM. From transcription to activation: how group A streptococcus, the flesh-eating pathogen, regulates SpeB cysteine protease production. Mol Microbiol. 2011 Jun 24. Carroll RK, Shelburne SA 3rd, Olsen RJ, Suber B, Sahasrabhojane P, Kumaraswami M, Beres SB, Shea PR, Flores AR, Musser JM. Naturally occurring single amino acid replacements in a regulatory protein alter streptococcal gene expression and virulence in mice. J Clin Invest. 2011 May 2;121(5):1956-68. Chandler WL, Peerschke EI, Castellone DD, Meijer P; on behalf of the NASCOLA Proficiency Testing Committee. von Willebrand Factor Assay Proficiency Testing: The North American Specialized Coagulation Laboratory Association Experience. Am J Clin Pathol. 2011 Jun;135(6):862-869. Chandler WL, Yeung W, Tait JF. A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer. J Thromb Haemost. 2011 Jun;9(6):1216-24. de la Roza G. p63 Expression in Giant Cell-Containing Lesions of Bone and Soft Tissue. Arch Pathol Lab Med. 2011 Jun;135(6):776-9. Deng Y, Wang Y, Wang J, Jing H, Yu C, Wang H, Liu Z, Graviss EA, Ma X. Laboratory-based surveillance of extensively drugresistant tuberculosis, China. Emerg Infect Dis. 2011 Mar;17(3):495-7. Continued page 8

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RECENT PUBLICATIONS continued El Sahly H, Teeter LD, Jost KC Jr, Dunbar D, Lew J, Graviss EA. Incidence of Moxifloxacin Resistance in Clinical Mycobacterium tuberculosis Isolates in Houston, Texas. J Clin Microbiol. 2011 Jun 8. [Epub ahead of print] Fudala R, Allen TC, Krupa A, Cagle PT, Nash S, Gryczynski Z, Gryczynski I, Kurdowska AK. Increased levels of nuclear factor ÎşB and Fos-related antigen 1 in lung tissues from patients with acute respiratory distress syndrome. Arch Pathol Lab Med. 2011 May;135 (5):647-54. Gao L, Zhou H, Thrall MJ, Li F, Yang Y, Wang Z, Luo P, Wong KK, Palapattu GS, Wong ST. Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy. Biomed Opt Express. 2011 Mar 18;2 (4):915-26. Garcia GE, Truong LD, Chen JF, Johnson RJ, Feng L. Adenosine A(2A) receptor activation prevents progressive kidney fibrosis in a model of immune-associated chronic inflammation. Kidney Int. 2011 Apr 20. [Epub ahead of print] Ge Y, Ro JY, Kim D, Kim CH, Reardon MJ, Blackmon S, Zhai J, Coffey D, Benjamin RS, Ayala AG. Clinicopathologic and immunohistochemical characteristics of adult primary cardiac angiosarcomas: analysis of 10 cases. Ann Diagn Pathol. 2011 May 3. [Epub ahead of print] Gomez P, Yorke R, Ayala AG, Ro JY. Solid-pseudopapillary neoplasm of pancreas with long delayed liver metastasis. Ann Diagn Pathol. 2011 Jun 3. [Epub ahead of print] Harvey AM, Mody DR, Amrikachi M. Disseminated blastomycosis diagnosed by fine-needle aspiration of the thyroid. Diagn Cytopathol. 2011 Jun;39(6):446-50. Ito M, Zhao N, Zeng Z, Chang CC, Zu Y. Interleukin-2 functions in anaplastic large cell lymphoma cells through augmentation of extracellular signal-regulated kinases 1/2 activation. Int J Biomed Sci. In press. Jang SJ, Gardner JM, Ro JY. Diagnostic approach and prognostic factors of cancers. Adv Anat Pathol. 2011 Mar;18(2):165-72. Kelly JD, Teeter LD, Graviss EA, Tweardy DJ. Intracranial tuberculomas in adults: A report of twelve consecutive patients in Houston, Texas. Scand J Infect Dis. 2011 Jun 22. [Epub ahead of print] Kim DY, Hong GU, Ro JY. Signal pathways in astrocytes activated by cross-talk between of astrocytes and mast cells through CD40CD40L. J Neuroinflammation. 2011 Mar 16;8:25. Kim DY, Kwon EY, Hong GU, Lee YS, Lee SH, Ro JY. Cigarette smoke exacerbates mouse allergic asthma through Smad proteins expressed in mast cells. Respir Res. 2011 Apr 18;12:49. Kim HJ, Kim MH, Kwon J, Kim JY, Park K, Ro JY. Proximal-type epithelioid sarcoma of the vulva with INI1 diagnostic utility. Ann Diagn Pathol. 2011 Jul 1. [Epub ahead of print] Kim MJ, Cho KJ, Ayala AG, Ro JY. Chondrosarcoma: with updates on molecular genetics. Sarcoma. 2011;2011:405437. Kim MP, Correa AM, Blackmon S, Quiroga-Garza G, Weilbaecher D, Bruckner B, Ramlawi B, Rice DC, Vaporciyan AA, Reardon MJ. Outcomes after right-side heart sarcoma resection. Ann Thorac Surg. 2011 Mar;91(3):770-6. Kwon Y, Ro J, Kang HS, Kim SK, Hong EK, Khang SK, Gong G, Ro JY. Clinicopathological parameters and biological markers predicting non-sentinel node metastasis in sentinel node-positive breast cancer patients. Oncol Rep. 2011 Apr;25(4):1063-71 Lassman AB, Iwamoto FM, Cloughesy TF, Aldape KD, Rivera AL, Eichler AF, Louis DN, Paleologos NA, Fisher BJ, Ashby LS, Ciarncross JG, Roldan GB, Wen PY, Ligon KL, Schiff D, Robins HI, Rocque BG, Chamberlain MC, Mason WP, Weaver SA, Green RM, Kamar FG, Abrey LE, Deangelis LM, Jhanwar SC, Rosenblum MK, Panageas KS. International retrospective study of over 1000 adults with anaplastic oligodendroglial tumors. Neuro Oncol. 2011 Jun;13(6):649-59. Lee EY, Ro JY. The 9th Spring Seminar of the Korean Pathologists Association of North America. Arch Pathol Lab Med. 2011 Jun;135 (6):696-7. Lewis AL, Truong LD, Zhai QJ. Benign Salivary Gland Tissue Inclusion in a Pulmonary Hilar Lymph Node in a Patient with Invasive Well-Differentiated Adenocarcinoma of the Lung. International Journal of Surgical Pathology. 2011 Jun;19(3):382-5. Massilamany C, Gangaplara A, Gardner DJ, Musser JM, Steffen D, Somerville GA, Reddy J. TCA cycle inactivation in Staphylococcus aureus alters nitric oxide production in RAW 264.7 cells. Mol Cell Biochem. 2011 Apr 26. [Epub ahead of print] Continued page 9

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RECENT PUBLICATIONS continued Musser JM.Third-track pathology: in unambiguous support of the Banbury conference report. Arch Pathol Lab Med. 2011 Jun;135 (6):687-8. Nese N, Martignoni G, Fletcher CD, Gupta R, Pan CC, Kim H, Ro JY, Hwang IS, Sato K, Bonetti F, Pea M, Amin MB, Hes O, Svec A, Kida M, Vankalakunti M, Berel D, Rogatko A, Gown AM, Amin MB. Pure epithelioid PEComas (so-called epithelioid angiomyolipoma) of the kidney: A clinicopathologic study of 41 cases: detailed assessment of morphology and risk stratification. Am J Surg Pathol. 2011;35:161-76. Olsen RJ, Watkins ME, Cantu CC, Beres SB, Musser JM. Virulence of serotype M3 Group A Streptococcus strains in wax worms (Galleria mellonella larvae). Virulence. 2011 Mar-Apr;2(2):111-9. Paner GP, McKenney JK, Barkan GA, Yao JL, Frankel WL, Sebo TJ, Shen SS, Jimenez RE. Immunohistochemical Analysis in a Morphologic Spectrum of Urachal Epithelial Neoplasms: Diagnostic Implications and Pitfalls. Am J Surg Pathol. 2011 Jun;35(6):787-798. Plata KB, Rosato RR, Rosato AE. Fate of Mutation Rate Depends on agr Locus Expression during Oxacillin-Mediated HeterogeneousHomogeneous Selection in Methicillin-Resistant Staphylococcus aureus Clinical Strains. Antimicrob Agents Chemother. 2011 Jul;55 (7):3176-86. Powers MP, Alvarez K, Kim HJ, Monzon FA. Molecular Classification of Adult Renal Epithelial Neoplasms Using MicroRNA Expression and Virtual Karyotyping. Diagn Mol Pathol. 2011 Jun;20(2):63-70. Ren X, Zhou X, Chang CC.The network properties of myelodysplastic syndromes pathogenesis revealed by an integrative systems biological method. Mol Biosyst. 2011 Jun;7(6):2048-54. Saloman JL, Niu KY, Ro JY. Activation of peripheral delta-opioid receptors leads to anti-hyperalgesic responses in the masseter muscle of male and female rats. Neuroscience. 2011 Jun 6. [Epub ahead of print] Schaaf CP, Blazo M, Lewis RA, Tonini RE, Takei H, Wang J, Wong LJ, Scaglia F. Early-onset severe neuromuscular phenotype associated with compound heterozygosity for OPA1 mutations. Mol Genet Metab. 2011 May 7. [Epub ahead of print] Shelburne III SA, Sahasrobhajane P, Suber B, Keith DB, Davenport MT, Horstmann N, Kumaraswami M, Olsen RJ, Brennan RG, Musser JM. Niche-specific contribution to streptococcal virulence of a MalR-regulated carbohydrate binding protein. Mol Microbiol. 2011 Jun 5. [Epub ahead of print] Sitkiewicz I, Green NM, Guo N, Mereghetti L, Musser JM. Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins. BMC Microbiol. 2011 Apr 1;11:65. Song C, Kang T, Yoo S, Jeong IG, Ro JY, Hong JH, Kim CS, Ahn H. Tumor volume, surgical margin, and the risk of biochemical recurrence in men with organ-confined prostate cancer. Urol Oncol. 2011 Jun 28. [Epub ahead of print] Wang EH, Truong LD, Mendoza L, Jung ES, Choi YJ. 28S-ribosomal RNA is superior to glyceraldehyde-3-phosphate dehydrogenase as a RNA reference gene in p53-deficient mice with unilateral ureteral obstruction. Exp Mol Pathol. 2011 May 2;91(1):368-372. Wang P, Hudspeth E. Increased body mass index but not common vitamin D receptor, PPARg and cytokine polymorphisms confer predisposition to post-transplant diabetes. Arch Pathol Lab Med. In Press. You D, Shim M, Jeong IG, Song C, Kim JK, Ro JY, Hong JH, Ahn H, Kim CS. Multilocular cystic renal cell carcinoma: clinicopathological features and preoperative prediction using multiphase computed tomography. BJU Int. 2011 Jul 1. [Epub ahead of print] Xia Z, Wen J, Chang CC, Zhou X. NSMAP: A method for spliced isoforms identification and quantification from RNA-Seq. BMC Bioinformatics. 2011 May 16;12:162. ď Žď&#x20AC;

The Laboratory Report The Laboratory Report is a publication of The Methodist Hospital System Department of Pathology and Laboratory Medicine.

Editor-in-Chief April A. Ewton, M.D. Editorial Committee Hazel L. Awalt, M.D. Christopher Leveque, M.D.

Claudia P. Molina, M.D. Seema Mullick, M.D. Thu Ngo, M.D. Steven Shen, M.D., Ph.D. Paul Sumby, Ph.D.

Ping Wang, Ph.D. Editorial Coordinator The Methodist Hospital Clare Rose, M.B.A. Philip Randall Manuel Hinojosa, M.H.A.