Enzyme-Linked ImmunoSorbent Assay See more with KPL ELISA products — Get higher signal and lower background. SEE MORE with KPL!
ELISA See more with KPL ELISA Products Sometimes it is difficult to distinguish your protein of interest from background. However, with KPL Protein DetectorTM ELISA products you get strong, clean signal every time. KPL’s ELISA products offer consistent, high quality results and enable sensitive immunodetection of protein in a microwell format. Choose from a broad line of sensitive substrates, high quality kits and stand-alone reagents for detecting and quantifying specific antigens in a complex protein mixture. Combine these products with KPL’s extensive line of secondary antibodies to develop reproducible and sensitive ELISAs. • • • •
Substrates for colorimetric or chemiluminescent detection Secondary antibodies unlabeled or labeled with either horseradish peroxidase (HRP) or alkaline phosphatase (AP) Support reagents – blocking solutions, coating buffers, wash solutions, conjugate stabilizers, stop solutions Universal ELISA kits formatted with HRP- or AP-labeled secondary antibodies
The foundation of KPL’s Protein Detector ELISA offering is a broad line of chemiluminescent and chromogenic substrates for use with HRP and AP conjugates that provide varied levels of sensitivity. For highly sensitive colorimetric peroxidase detection, choose SureBlue Reserve™ Microwell Substrate with long shelf life and convenient 1-component format. New FirePhos™ Microwell Substrate expands our selection of sensitive substrates for phosphatase detection. LumiGLO Reserve™ Chemiluminescent Substrate offers the ultimate in sensitivity via chemiluminescent reaction with HRP. No matter which you choose, KPL’s ELISA substrates are all tested to assure lot-to-lot consistency, reproducibility and long-term stability. An extensive line of high-quality secondary antibody conjugates and labeled streptavidin are also available. Each is affinity purified to assure excellent signal-to-noise results in ELISAs. KPL support reagents are optimized for use in microwell ELISA and tested to verify that they meet strict specifications. They include conjugate stabilizers, diluents, blocking and wash solutions, all formulated to ensure accurate and reproducible results. Protein Detector Kits provide a convenient starting point for the development of ELISA protocols. Each kit contains enzyme-labeled secondary antibody, corresponding substrate, as well as unique diluent/blocking and wash solutions.
KPL ELISA products help you to optimize your assay and SEE MORE!
1.800.638.3167
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Praying Mantis
See more with KPL ELISA products — Get higher signal and lower background.
ELISA ELISA Substrates Substrates are the cornerstones for developing ELISAs with high signal and low background. KPL, one of the first companies to produce stable, liquid substrates, offers a variety of standard and proprietary HRP and AP substrates. See Table 1 for a listing of chromogenic and chemiluminescent substrates.
stability, and consistency, making it the ideal substrate for use in most ELISA applications. Superior Sensitivity with SureBlue Reserve
• •
Chromogenic Peroxidase Substrates
• •
Choose from among three TMB (3,3’,5,5’tetramethylbenzidine) products and two ABTS (2,2”-azino-di-(3-ethylbenzthiazoline -6-sulfonate) formulations. All provide guaranteed performance and reliability. Excellent Signal-to-Noise with SureBlueTM
• • •
High sensitivity Consistent lot-to-lot performance minimizes assay re-optimization 2-year stability
KPL’s SureBlue TMB Microwell Substrate is a 1-component formulation. It develops a deep blue soluble product when reacted with HRP-labeled conjugates. SureBlue TMB provides excellent signal-to-noise,
Highest sensitivity: 50% more sensitive than SureBlue TMB (Figure 1) Consistent lot-to-lot performance minimizes assay re-optimization 2-year stability Ready-to-use 1-component formulation
SureBlue Reserve TMB takes ELISA to a new performance level. It offers maximum sensitivity with exceptionally low background enabling detection of low levels of target protein. SureBlue Reserve TMB provides the same great benefits as KPL’s original SureBlue TMB: low lot-to-lot variation, 2-year stability, ready-to-use 1-component solution, and excellent linearity. TMB Microwell Substrate in a Highly Stable 2-Component Formulation
• •
Longest shelf life due to the 2-component system Higher sensitivity than ABTS
TMB Microwell 2-Component Substrate offers excellent stability and sensitivity. It is the most economical choice when ready-to-use convenience is not required. Like other TMB substrates, it rapidly develops a deep blue color that is measurable at 650 nm. Broad Dynamic Range with ABTS Microwell Substrates
•
• •
Excellent linearity; provides the widest working range of any chromogenic HRP substrate Extremely stable Ideal for kinetic assays
KPL’s ABTS Microwell Substrates offer performance, low background, and a wider linear range than other chromogenic substrates. Supplied either as a ready-to-use formulation or as an extremely stable 2-component product, ABTS substrates develop an intense blue-green color in the presence of peroxidase-labeled conjugates. ABTS is recommended when low background is required and moderate sensitivity is acceptable.
Table 1. KPL Protein Detector ELISA Substrates SureBlue Reserve SureBlue TMB 2-C ABTS 1-C ABTS 2-C BluePhos FirePhos pNPP LumiGLO Reserve LumiGLO PhosphaGLO PhosphaGLO Reserve
Sensitivity ••• •• •• • • •• •• • ••••• •••• •••• •••••
Stability ••• ••• •••• •••• ••••• •• •• ••••• •• •• ••• •••
C = Component
Convenience ••••• ••••• ••• ••••• ••• ••• ••• • ••• ••• ••••• ••••• • = Lowest
Enzyme HRP HRP HRP HRP HRP AP AP AP HRP HRP AP AP
Detection Chromo Chromo Chromo Chromo Chromo Chromo Chromo Chromo Chemi Chemi Chemi Chemi
••••• = Highest
Determination Kinetic or Endpoint Kinetic or Endpoint Kinetic or Endpoint Kinetic or Endpoint Kinetic or Endpoint Kinetic or Endpoint Kinetic or Endpoint Kinetic or Endpoint Endpoint Endpoint Kinetic or Endpoint Kinetic or Endpoint
Comparison of SureBlue and SureBlue Reserve
Comparison of AP Colorimetric Substrates for ELISA
1.0 0.5 0.0 0
0.5
BluePhos FirePhos pNPP
20
SureBlue Reserve SureBlue
1.5
Signal: Background
O.D. at 450 nm
2.0
1
1.5
15 10 5
2 0
HRP Concentration (ng/mL)
For economical chromogenic phosphatase detection that provides sensitivity and convenience, KPL offers three substrates that produce a variety of colors, new FirePhos, BluePhos® and pNPP Substrates. Excellent Overall Performance
• • • •
pNPP Phosphatase Substrate contains para-nitrophenylphosphate. KPL’s formu-
• •
Wide linear range Excellent stability
1000
LumiGLO and LumiGLO Reserve substrates are luminol-based. In the presence
endpoint determinations can be performed. It is available in tablet form with a separate buffer solution for shelf stability.
to an excited intermediate dianion which
Figure 3. A Choice of Colors for Phosphatase Detection
of hydrogen peroxide, they are converted emits light on return to its ground state. Both LumiGLO Reserve and LumiGLO substrates offer rapid and sensitive detection. The working range of primary antibody that yields an acceptable signal-tonoise ratio is much wider with these substrates than with chromogenic substrates. Both substrates are supplied as 2-component liquids. PhosphaGLOTM and PhosphaGLO
RedPhos
Economy and Performance with pNPP
800
600
lation provides one of the most sensitive pNPP substrates available. Both kinetic and
Ideal for applications where sensitivity greater than pNPP is required Stable for 24 months Convenient and quick: mix and use Wider dynamic range than pNPP Lower background than pNPP
FirePhos and BluePhos Microwell Substrates are based on a soluble, proprietary form of 5-bromo-4-chloro-3indolyl phosphate (BCIP) and produce intense colors (Figure 3). Both substrates offer greater sensitivity and linearity of signal than pNPP (Figure 2) and can be used for both kinetic and endpoint ELISAs.
400
Figure 2. Human IgG was serially diluted in an ELISA plate and detected with goat ani-human IgG labeled with alkaline phosphatase. KPL’s substrates, FirePhos, BluePhos and pNPP were added to the plate. The reactions were stopped with APstop Solution and read at the recommended wavelength for each substrate. The results indicate that FirePhos and BluePhos produce a significantly higher signal-to-noise relative to pNPP.
with FirePhosTM and BluePhos®
•
200
Antigen Concentration (ng/mL)
Figure 1. Dilutions of HRP conjugate were added to microplate wells and the conjugate in each well reacted with either SureBlue or SureBlue Reserve. The reactions were stopped with TMB Stop Solution, and optical density readings were taken at 450 nm. Data points represent the average of triplicate samples at each HRP concentration. The data demonstrate greater sensitivity of SureBlue Reserve at low concentrations of HRP.
Chromogenic Phosphatase Substrates
0
BluePhos
pNPP
ReserveTM Chemiluminescent Phosphatase Substrates
Chemiluminescent Substrates for Peroxidase and Phosphatase
• Femtogram sensitivity with PhosphaGLO Reserve • One-component, ready-to-use • Stable at 4o C for two years.
LumiGLO® and LumiGLO Reserve Chemiluminescent Peroxidase
PhosphaGLO Reserve and PhosphaGLO
Substrates
substrates provide great sensitivity and
• Unsurpassed sensitivity with LumiGLO Reserve • Excellent stability • Fast ramp-up of signal • Stable for 24 hours after mixing • Light emission sustained for 1-2 hours
unmatched convenience. No special blockers are required to achieve low background.
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ELISA See More...
ELISA Antibody Conjugates Choose from a wide selection of secondary antibodies to immunoglobulins from over 20 animal species labeled with horseradish peroxidase or alkaline phosphatase, the most widely used enzymes for ELISA signal generation. HRP has been shown to be more sensitive than AP primarily due to its faster catalytic rate. However, HRP reactions may be self-limiting due to substrate inhibition of enzyme. In contrast, AP exhibits a slower catalytic rate, but is not self-limiting. Reaction rates remain linear over long periods of time; therefore, sensitivity can be improved by allowing the reaction to proceed for a longer time. Whatever enzyme conjugate you choose, all antibody conjugates are of the same exceptionally high quality you have come to expect from KPL. As always, you get results with high signal-to-noise.
Universal ELISA Kits KPL’s Protein Detector ELISA Kits are designed to be an excellent and convenient starting point for development of ELISA protocols. Each kit contains all the reagents necessary to establish an ELISA procedure except for primary antibodies. The kits also include a comprehensive manual to facilitate assay development.
HRP ELISA Kits
KPL’s Protein Detector HRP ELISA Kits contain goat anti-mouse IgG and anti-rabbit IgG, or anti-human IgG HRP conjugates along with ABTS substrate, blocking, coating, substrate, stop and wash solutions. AP ELISA Kits
Included in the Protein Detector AP ELISA kits are goat anti-mouse IgG, anti-rabbit IgG, or anti-human IgG AP conjugates as well as BluePhos substrate, blocking, coating, stop and wash solutions.
ELISA Support Reagents
Try KPL’s ELISA Kits and Reagents KPL has extensive experience in antibody purification and the development of stable liquid substrates. This long-standing expertise is the foundation of our line of Protein Detector ELISA systems and ensures reliable product performance from lot-to-lot. Try our kits and reagents and get the results you’re looking for: a strong, clean signal without background, every time. Your eyes aren’t playing tricks on you. You’ve found the finest line of ELISA systems and reagents at KPL.
KPL’s support reagents are optimized for use in microwell ELISA and tested to verify that they meet strict specifications. They include conjugate stabilizers, diluents, blocking and wash solutions, and stop reagents. Blocking and wash solutions have been designed to reduce background and to ensure a clear signal. KPL stabilizers provide convenient storage solutions for your antibody-enzyme conjugates and ensure their performance. Spend your time developing your assay instead of worrying about making reagents. Choose time-tested, stable support reagents from KPL. LumiGLO and BluePhos are registered trademarks and LumiGLO Reserve, PhosphaGLO, PhosphaGLO Reserve, SureBlue Reserve, SureBlue, FirePhos, ReserveAP, BlueSTOP and Protein Detector are trademarks of KPL, Inc.
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HRP ELISA Kits Protein DetectorTM Horseradish Peroxidase (HRP) ELISA Kit • • • •
Antibody Sandwich (Capture) ELISA Indirect Antibody ELISA Direct ELISA Hybridoma Screening
Protein Detector ELISA kits offer the following benefits:
Protein DetectorTM ELISA Kit HRP System offers a convenient starting point for the development of microwell procedures for antigen or antibody detection. The kit provides pretested components including HRP-labeled anti-mouse and anti-rabbit or anti-human secondary antibodies along with blocking, coating and wash solutions which substantially reduce the time and effort needed to develop a reproducible assay.
Excellent linearity: ABTS provides the widest working range of any chromogenic HRP substrate.
This kit also contains ABTS®, a peroxidase
Non-hazardous, no special handling or disposal required. Optimized liquid stystem makes your ELISA experiments faster and easier. Ideal for use in a variety of ELISA techniques.
substrate that provides reliability, convenience and excellent overall performance. A comprehensive manual for developing enzyme immunoassay procedures is also provided. Users only need to provide primary antibody and plates to run an ELISA.
Excellent Dynamic Range
Extremely Stable All kit components are stabilized for ease-ofuse and reliability. Very low background, long shelf life. Convenient
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HRP ELISA Kits Features of ABTS Substrate
Performance of ABTS Substrate 0.500 ABTS unstopped
Substrate
ABTS
Type
Chromogenic
Kit Components
2
Detection Limit
10-13 moles of HRP
Kinetics
Slow
ABTS stopped
Absorbance
0.400
0.300
Stability of Working Solution 24 hours 0.200
0.100
7.8
15.6
31.3
62.5
125
250
500
1. Coating Solution Concentrate 2. BSA Diluent/Blocking Solution Concentrate 3. Wash Solution Concentrate
Hazards
None
Color Before Stopping
Blue-Green
4. 50% glyceral
Absorbance Before Stopping
405-410 nm
5. HRP-labeled antibody, .1 mg
Stop Reagent
ABTS Stop Solution
Color After Stopping
Blue-Green
Absorbance After Stopping
405-410 nm
0.000
Conjugate Concentration (ng/mL)
Each kit contains:
Amplification After Stopping None
6. 2-component ABTS Substrate 7. Stop Solution Concentrate 8. Manual
ABTS offers a broad working range prior to stopping and after stopping.
Storage: Store kit reagents at 2 - 8° C.
Stability: Kit is stable for a minimum of 1 year from date of receipt.
Ordering Information Catalog No.
Description
Size
54-62-10
Protein Detector ELISA Kit, HRP System Anti-Human Antibody
2000 tests
54-62-18
Protein Detector ELISA Kit, HRP System
2000 tests
Anti-Mouse Antibody 54-62-15
Protein Detector ELISA Kit, HRP System
2000 tests
Anti-Rabbit Antibody Related Products 55-81-50
Protein Detector ELISA Kit, AP System Anti-Mouse and Anti-Rabbit Antibodies
2000 tests
55-81-10
Protein Detector ELISA Kit, AP System Anti-Human Antibody
2000 tests
Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169
To order or for more information on KPL’s full line of protein and nucleic acid detection products, contact us at 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com.
www.kpl.com
ISO 9001:2000 Registered
ML285-04
ABTS is a registered trademark of the Roche Group. © 2003 KPL, Inc. All rights reserved.
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AP ELISA Kits Protein DetectorTM Alkaline Phosphatase (AP) ELISA Kits • • • •
Antibody Sandwich (Capture) ELISA Indirect Antibody ELISA Direct ELISA Hybridoma Screening
Protein Detector AP ELISA kits offer the following benefits: Highly Sensitive
Protein Detector AP ELISA Kits offer an excellent and convenient starting point for the development of ELISA protocols. These kits provide pretested goat anti-mouse, antirabbit or anti-human AP conjugates as well as blocking, coating and wash solutions. KPL kits substantially reduce the effort needed to develop a sensitive and reproducible assay. All components needed to establish an ELISA except for primary antibodies are provided.
Detect at least 2 ng of protein and less than 0.5 pg of phosphatase in free solution.
AP ELISA kits utilize BluePhos®, a phosphatase substrate that provides superior performance over pNPP. A comprehensive manual for developing enzyme immunoassay procedures is also provided.
Optimized liquid system makes your ELISA experiments faster and easier. Nonhazardous, no special handling or disposal required. Ideal for use in a variety of ELISA techniques.
Extremely Stable Very low background, long shelf life. Excellent Dynamic Range BluePhos offers improved signal-to-noise ratio and linearity over pNPP. Convenient
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Performance Comparison of BluePhos and pNPP
AP ELISA Kits Methodology
3.0 BluePhos
2.5
pNPP
O.D.
2.0
1.5
1.0
0.5
0 1
2
3
4
5
6
7
8
Natural Log (Dilution)
Ordering Information Catalog No.
Description
Size
55-81-50
Protein Detector ELISA Kit, AP System Anti-Mouse and Anti-Rabbit Antibodies
2000 tests
Protein Detector ELISA Kit, AP System Anti-Human Antibody
2000 tests
54-62-10
Protein Detector ELISA Kit, HRP System Anti-Human Antibody
2000 tests
54-62-18
Protein Detector ELISA Kit, HRP System Anti-Mouse Antibody
2000 tests
54-62-15
Protein Detector ELISA Kit, HRP System
2000 tests
55-81-10
Mouse IgG was coated onto two different ELISA plates. The starting dilution for the BluePhos plate was 1 mg/mL, while the pNPP plate started at 10 μg/mL. The antigen was diluted 2-fold eight times. Phosphatase-labeled goat antimouse IgG was added to each well at a concentration of 500 ng/mL. The plate was washed, and substrate applied. Based on the background readings at time 0, and the final readings at time 15 minutes, the amount of protein was determined by dividing the dilution concentration by 100 μL (the volume used when coating the well). Therefore, the amount of protein detected by BluePhos (point 7) is 1.56 ng, while the amount detected by pNPP (point 5) is 62.4 ng. Conclusion
Related Products
The graph shows the superiority of BluePhos in both increased sensitivity and in producing a wider dynamic range than pNPP.
Anti-Rabbit Antibody Each kit contains: Coating Buffer Concentrate, BSA Diluent/Blocking Solution Concentrate, Wash Solution Concentrate, 50% Glycerol Solution, Enzyme-labeled Secondary Antibody, 2-Component Chromogenic Substrate System (Substrate components may vary depending upon kit.), and Stop Solution.
Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169 www.kpl.com
To order or for more information on KPL’s full line of protein and nucleic acid detection products, contact us at 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com.
ISO 9001:2000 Registered
BluePhos is a registered trademark and Protein Detector is a trademarks of KPL, Inc. © 2003 KPL, Inc. All rights reserved.
ML271-06
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Immunoassay Buffers Save preparation time with KPL’s new line of quality buffers! Lighten your work load with KPL’s new line of quality
registered quality management system. All buffers are con-
buffers. Offered as convenient liquid concentrates, they
ductivity controlled. KPL offers a variety of buffers designed
eliminate the need for time-consuming buffer preparation.
for use in general immunoassay applications such as Western
KPL buffers are manufactured and carefully controlled for
blotting, gel electrophoresis, ELISA and sample preparation.
quality and consistent performance with our ISO 9001:2008Description
1X Composition
Applications
10X Tris-Glycine Transfer Buffer
25 mM Tris, 192 mM glycine. pH 8.3
10X Tris-Glycine-SDS
25 mM Tris, 192 mM glycine, 0.1% SDS. pH 8.3
For preparing a standard Western blot transfer buffer (Towbin) and as a gel electrophoresis buffer for native Tris-glycine gels without SDS. . Running buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins.
10X Phosphate-Buffered Saline (PBS)
10 mM Phosphate,150 mM NaCl. pH 7.4
Phosphate-Buffered Tween 20, (PBST)
10 mM Phosphate, 150 mM NaCl, 0.05% Tween 20. pH 7.4
10X Tris-Buffered Saline (TBS)
50 mM Tris, 150 mM NaCl. pH 7.6
10% Tween 20
10% Tween 20
Wash buffer for general immunoassay applications and sample preparation. Where applicable, Tween 20 is a nonionic detergent that reduces nonspecific binding and protein-protein interaction during wash steps.
Nonionic detergent additive for PBS and Western blotting wash solutions. Reduces nonspecific binding and proteinprotein interactions.
See reverse side.
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Immunoassay Buffers
KPL Quality Buffers (cont’d) Description
1X Composition
Applications
5% SDS
5% Sodium Dodecyl Sulfate
Detergent surfactant used in preparing proteins for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) and as an additive to transfer buffers in Western blotting. SDS increases the elution rate of proteins from the gel.
20X SSC
15 mM sodium citrate, 150 mM NaCl in DEPC water. pH 7.0
Used in a variety of molecular biology applications. Facilitates transfer of nucleic acids to membranes.
10X Dulbecco's PBS (D-PBS)
8.5 mM sodium phosphate, 1.5 mM potassium phosphate, 137 mM NaCl. pH 7.4
For sample preparation and as a wash buffer in general immunoassay, tissue, and cell culture applications. Not formulated with magnesium or calcium salts. Where applicable, Tween 20 is a non-ionic detergent that reduces nonspecific binding.
Other Immunoassay Support Reagents Available from KPL ELISA and Western Blotting Applications: • AP and HRP Conjugate Stabilizers
10X Dulbecco's with Tween 20. (D-PBST)
8.5 mM sodium phosphate, 1.5 mM potassium phosphate, 137 mM NaCl, 2.7 mM KCl. 0.05% Tween 20. pH 7.4
10X Tris-Buffered Saline with 0.5% Tween 20 (TBST)
50 mM Tris, 150 mM NaCl, 0.05% Tween 20. pH 7.6
10X Tris-Buffered Saline with 10% Tween 20 (TBST)
50 mM Tris, 150 mM NaCl, 1.0% Tween 20. pH 7.6
• 10% BSA Diluent/Blocking Solution Concentrate • Milk/Diluent Blocking Solution Concentrate • 20X Wash Solution Concentrate
As a general wash buffer in immunoassay applications. Tween 20 is a non-ionic detergent that reduces nonspecific binding. The 10% Tween 20 formulation provides a more stringent wash than standard TBST formulations.
Western Blotting Applications: • Detector Block • Biotin Wash Kit
Take the time and effort out of your buffer preparation. Choose from our line of popular buffers and enjoy the benefits - convenience, reliability and confidence - that KPL’s pretested, quality buffers bring to your research.
ELISA Applications: • Coating Solution Concentrate • Stop Solutions
Ordering Information Catalog#
Description
Size
51-10-01 51-10-02 51-11-01 51-11-02 51-13-01 51-13-02 51-14-01 51-14-02 51-17-01 51-17-02 51-12-01 51-12-02 51-20-01 50-86-05 51-15-01 51-15-02 51-16-01 51-16-02 51-18-01 51-18-02 51-19-01 51-19-02
10X Tris-Glycine Transfer Buffer
1L 5L 1L 5L 1L 5L 200 mL 1L 1L 5L 200 mL 1L 200 mL 1L 1L 5L 200 mL 1L 200 mL 1L 200 mL 1L
10X Tris-Glycine SDS Buffer 10X Phosphate-Buffered Saline (PBS) 10X Phosphate-Buffered Saline with Tween 20 (PBST) 10X Tris-Buffered Saline (TBS) 10% Tween 20 5% SDS 20X SSC 10X Dulbecco's PBS (D-PBS) 10X Dulbecco's PBS with Tween 20 (D-PBST) 10X Tris Buffered Saline with 0.5% Tween 20 (TBST) 10X Tris Buffered Saline with 10% Tween 20 (TBST)
Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169 www.kpl.com
ISO 9001:2008 Registered
To order or for more information on KPL’s full line of protein detection products, contact us at 800.638.3167 / 301.948.7755, fax 301.948.0169 or visit us at www.kpl.com. ©2009 KPL, Inc. All rights reserved. ML363-03
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HRP ELISA Color Substrates Reliable performance and a range of options The success of an ELISA (enzyme-linked immunosorbent assay) depends on a number of factors, including the performance characteristics of the substrate. KPL’s line of peroxidase (HRP) color substrates provides a range of options to match individual perform-
sulfonate) peroxidase substrates offer reliable performance and low background in ELISA. They turn an intense blue-green in the presence of peroxidase that is measureable between 405 and 415 nm.
Choose the substrate that suits your needs:
ance requirements, including high signal and sensitivity, low background, stability and convenience. As the most preferred peroxidase substrate, TMB (3,3’,5,5’ tetramethylbenzidine) provides high sensitivity and low background in microwell immunoassays. KPL offers a choice of three TMB substrates, starting with SureBlueTM (1-Component), ideal for most ELISA applications. Our TMB Peroxidase
• SureBlue ReserveTM TMB Substrate SureBlue Reserve provides maximum sensitivity while maintaining low background, high consistency and 24-month stability in a convenient 1-component formulation.
• SureBlueTM TMB Microwell Substrate SureBlue TMB offers high sensitivity, consistency and stability in a convenient 1-component solution.
Substrate (2-Component) provides equivalent sensitivity at an economical price. At the high end of sensitivity, SureBlue Reserve
TM
(1-Component ) is the
substrate of choice when ultimate performance is required. All three substrates are recommended for
• TMB Peroxidase Substrate TMB Peroxidase Substrate offers equivalent performance to SureBlue in an easy mix-and-use, 2-component formulation.
both kinetic and endpoint ELISA. Upon reaction
• ABTS Peroxidase Substrate
with peroxidase, they turn blue and are read at 650
ABTS substrates are supplied as stable 1- and 2-component formulations. They are recommended when low background is required and moderate sensitivity is acceptable.
nm. KPL’s ABTS (2,2’-azino-di-(3-ethylbenzthiazoline-6-
SEE MORE with KPL!
TMB Stop Solutions Comparison of KPL’s HRP Microwell Substrates • TMB BlueSTOPTM - formulated to stop color development in microwell assays and to maintain the blue color of TMB. Unlike traditional acid-based TMB stopping solutions, the O.D. value of TMB does not change upon addition of TMB BlueSTOP. The stopped substrate is stable for an extended period for reading at 650 nm. A single 650 nm filter can be used pre- and post-stopping. As the graph above demonstrates, our TMB substrates (SureBlue Reserve, SureBlue and TMB) produce high O.D. values, with SureBlue Reserve exhibiting the highest sensitivity. SureBlue and TMB demonstrate lower O.D. values and are comparable in performance. ABTS 1- and 2-component yield lower O.D. values than TMB while exhibiting similar performance. In summary, KPL’s colorimetric substrates provide a range of sensitivity options to meet a variety of needs.
Ordering Information Catalog# 52-00-01 52-00-02 52-00-03 53-00-01 53-00-02 53-00-03 50-76-00 50-76-03 50-66-18 50-66-00 50-66-06 50-62-00 50-62-01
Description SureBlue TMB Microwell Substrate (1-Component) SureBlue TMB Microwell Substrate (1-Comp) SureBlue TMB Microwell Substrate (1-Comp) SureBlue Reserve TMB Microwell Substrate (1-Comp) SureBlue Reserve TMB Microwell Substrate (1-Comp) SureBlue Reserve TMB Microwell Substrate (1-Comp) TMB Microwell Substrate (2-Comp) TMB Microwell Substrate (2-Comp) ABTS Microwell Substrate (1-Comp) ABTS Microwell Substrate (1-Comp) ABTS Microwell Substrate (1-Comp) ABTS Substrate System (2-Comp) ABTS Substrate System (2-Comp)
50-85-30 50-85-41 50-85-05 50-85-06 50-85-01
Stop Solutions TMB BlueSTOP Solution TMB BlueSTOP Solution TMB Stop Solution TMB Stop Solution ABTS Stop Solution
Size 100 mL 400 mL 1L 100 mL 400 mL 1L 600 mL 2700 mL 100 mL 600 mL 1L 600 mL 2700 mL
400 mL 1L 400 mL 1L 200 mL
• TMB Stop Solution - changes the blue color of TMB substrate to yellow and increases absorbance values 2 - 3 times. Stopped TMB is read at a wavelength of 450 nm.
• ABTS Stop Solution - specifically designed for use with KPL’s ABTS Peroxidase Substrates to stop color development in micro-well assays. ABTS retains the green color after stopping.
Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169 www.kpl.com
ISO 9001:2000 Registered
To order or for more information on KPL’s line of protein detection products, contact us at 800.638.3167 / 301.948.7755, fax 301.948.0169 or visit us at www.kpl.com. SureBlue, SureBlue Reserve and TMB BlueSTOP are trademarks of KPL. ABTS is a registered trademark of Roche Biochemicals. ©2008 KPL, Inc. All rights reserved. ML352-02 SEE MORE with KPL!
AP ELISA Color Substrates See your ELISA results in vivid colors! Prefer vibrant red or brilliant blue? Choose between new FirePhosTM, BluePhos® or pNPP AP Microwell Substrates for sensitive ELISA detection! KPL offers a choice of three sensitive substrates for the detection and quantification of phosphatase (AP) activity in microwell assays. New FirePhosTM AP Microwell Substrate BluePhos® AP Microwell Substrate pNPP Phosphatase Substrate Whichever substrate you choose, enjoy the benefits of excellent signal-to-noise with higher sensitivity and lower background than that of other AP microwell substrates. They produce strong colors that can be read visually. When combined with KPL’s APstopTM Solution, signal development is completely stopped for endpoint determinations. The fast rate of color generation also permits accurate, quantitative measurement in kinetic ELISA.
Extended stability All three AP substrates are stable for one year. Convenience FirePhos and BluePhos are offered as liquid two-component solutions. To prepare mix equal parts and use. Sensitivity FirePhos and BluePhos provide greater sensitivity than pNPP and can detect less than 0.5 pg of phosphatase. (See data on reverse side.) KPL’s pNPP substrate is one of the most sensitive pNPP formulations available, showing enhanced performance over competitive products. The color produced is stable and linear over a wide dynamic range. Reproducibility All KPL AP colorimetric substrates are optimized for consistent results from lot-to-lot, assay-to-assay. SEE MORE with KPL!
ReserveAP Conjugates
Phosphatase Substrates for ELISA
TM
Improve your assay performance even further with high potency ReserveAPTM Antibodies KPL’s new ReserveAPTM Alkaline Phosphatase-labeled Conjugates exhibit high potency and consistent performance in immunoasComparison of AP colorimetric substrates for ELISA Human IgG was serially diluted in an ELISA plate and detected with goat anti-human IgG labeled with alkaline phosphatase. KPL’s substrates, FirePhos, BluePhos, pNPP and a competitor pNPP were added to the plate. The reactions were stopped with APstop Solution and read at the recommended wavelength for each substrate. The results indicate that FirePhos and BluePhos produce a significantly higher signal-to-noise relative to pNPP.
result of advances in our conjugation technology and offer higher signal and lower background than our current line of alkaline phos-
Ordering Information Catalog#
says. These conjugates are the
phatase conjugates while meeting
Description
Size
the same standards for stability and reproducibility. They are ideal
FirePhosTM AP Microwell Substrate
for immunoassays that require
50-87-10
FirePhos Microwell Phosphatase Substrate System
50 mL
50-87-20
FirePhos Microwell Phosphatase Substrate System
200 mL
high detection sensitivity in an AP
50-87-30
FirePhos Microwell Phosphatase Substrate System
600 mL
system, including ELISA, Western
50-87-40
FirePhos Microwell Phosphatase Substrate System
2.7 L
blotting and immunohistology.
BluePhos® AP Microwell Substrate 50-88-02
BluePhos Phosphatase Substrate for ELISA
50 mL
50-88-00
BluePhos Phosphatase Substrate for ELISA
600 mL
50-88-01
BluePhos Phosphatase Substrate for ELISA
2700 mL
pNPP Phosphatase Substrates 50-80-00
pNPP Phosphatase Substrate Kit, 100 tabs & DEA Buffer
500 mL
50-80-01
pNPP Substrate, 5 mg tablets
100 tabs
APstop Solution (10X) (formerly BluePhos Stop Solution)
200 mL
Stop Solutions 50-89-00
Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169 www.kpl.com ISO 9001:2000 Registered
To order or for more information, contact us at 800.638.3167 / 301.948.7755, fax 301.948.0169 or visit us at www.kpl.com. ML347-02 For research use only. BluePhos is a trademark of KPL and is covered by U.S. Patent No. 5,916,746. © 2007 KPL, Inc. All rights reserved.
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ReserveAP Conjugates TM
TM
Spice up your assay with our red hot high potency ReserveAP Conjugates! KPL’s new ReserveAPTM Alkaline Phosphatase
line of AP conjugates in ELISA and outperform
(AP)-labeled antibody conjugates exhibit high
AP conjugates offered by other manufacturers.
potency and consistent performance in
Higher conjugate dilutability is also observed
immunoassays. These conjugates are the result
without loss of linearity, enabling precious
of advances in our conjugation technology and
antigen or primary antibody conservation.
offer higher signal than our current line of AP
Consistent Performance
conjugates while meeting the same standards for low background, stability and reproducibili-
Reproducible antibody conjugation and consis-
ty. They are intended for demanding
tent performance are verified according to our
immunoassays that require high detection sen-
ISO 9001:2000-registered quality assurance
sitivity, including ELISA, Western blotting and
system. Lot consistency studies in which three
immunohistology.
lots were studied by ELISA indicated minimal variability.
Higher Potency Excellent Value ReserveAP Conjugates are affinity purified and conjugated to the highest grade of alkaline
ReserveAP Conjugates provide high perform-
phosphatase. In our studies, they generate two-
ance at an economical price.
to-three fold higher values than our current SEE MORE with KPL!
AP Substrates Chemiluminescent ELISA: A constant amount of antigen (mouse IgG) was coated onto a microtiter plate. The plate was probed with varying concentrations of goat anti-mouse IgG (H+L) AP conjugate, including KPL’s standard AP conjugate and ReserveAP™ conjugate, followed by KPL’s PhosphaGLO™ Chemiluminescent AP Substrate.
KPL offers a range of sensitive substrates for the detection and quantification of phosphatase (AP) activity. They provide a choice of intense colors for ELISA and blotting applications.
ELISA •
Conclusion: The results indicate that ReserveAP™ conjugate offers superior performance to our standard AP conjugate with a ~3-fold lower amount of conjugate required for detection.
ReserveAPTM Conjugates Ordering Information
•
•
Catalog#
Description
Size
0751-1001
Goat Anti-Human IgA (α)
1.0 mg
0751-1002
Goat Anti-Human IgG (γ)
1.0 mg
0751-1003 0751-1004
Goat Anti-Human IgM (µ) Goat Anti-Human IgE (ε)
0751-1006
FirePhosTM AP Microwell Substrate BluePhos® AP Microwell Substrate pNPP Phosphatase Substrate
Blotting •
1.0 mg 1.0 mg
FirePhos AP Membrane Substrate
•
BCIP/NBT Phosphatase Substrate
Goat Anti-Human IgG (H+L)
1.0 mg
•
PhosphaGLO AP Substrate
0751-1007
Goat Anti-Human IgA+IgG+IgM (H+L)
1.0 mg
•
2151-1002
0.5 mg
4751-1002
F(ab’)2 Anti-Human IgG (γ) Goat Anti-Human IgG (γ) liquid
PhosphaGLOTM Reserve AP Substrate
4751-1003
Goat Anti-Human IgM (µ) liquid
1.0 mg
4751-1006
Goat Anti-Human IgG (H+L) liquid
1.0 mg
0751-1802
Goat Anti-Mouse IgG (γ) HSA
1.0 mg
0751-1803
Goat Anti-Mouse IgM (µ) HSA
1.0 mg
0751-1806
Goat Anti-Mouse IgG (H+L) HSA
1.0 mg
0751-1809
Goat Anti-Mouse IgG +IgM (H+L) HSA
1.0 mg
4751-1802
Goat Anti-Mouse IgG (γ) HSA, liquid
1.0 mg
4751-1806
Goat Anti-Mouse IgG (H+L) HSA, liquid
1.0 mg
1.0 mg
151-18-01
Goat Anti-Mouse IgA (α) HSA
0.5 mg
0751-1807
Goat Anti-Mouse IgA+IgG+IgM (H+L) HSA
1.0 mg
0751-1506
Goat Anti-Rabbit IgG (H+L)
1.0 mg
0751-1516
Goat Anti-Rabbit IgG (H+L) HSA
1.0 mg
4751-1506
Goat Anti-Rabbit IgG (H+L), liquid
1.0 mg
4751-1516
Goat Anti-Rabbit IgG (H+L) HSA, liquid
1.0 mg
Whichever substrate you choose, enjoy the benefits of excellent signalto-noise with higher sensitivity and lower background than that of other AP substrates. Visit www.kpl.com for more information.
Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169 www.kpl.com ISO 9001:2000 Registered
HSA=Human Serum Adsorbed
Visit our website at www.kpl.com for a complete listing of ReserveAP conjugates. To order or for more information on KPL’s protein research products, contact us at 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com. ML349-04 For research use only. ©2007 KPL, Inc. All rights reserved.
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BacTrace® Anti-Bacteria Antibodies BacTrace® E.coli O157:H7 Antibody labeled with DyLight 488 (green) directly detects E.coli O157:H7. BacTrace Salmonella (CSA-1) Antibody labeled with DyLight 549 (red) directly detects Salmonella. (DyLight labeling performed on a custom basis.)
BacTrace Antibodies to Food Pathogens and Other Infectious Agents KPL produces a unique line of BacTrace® antibacteria antibodies for the detection of food pathogens and other infectious agents affecting humans and animals. They are purified to offer excellent specificity and sensitivity in high performance immunoassays such as ELISA, Western blotting, lateral flow and microscopic analysis. Many leading diagnostic kit manufacturers use BacTrace antibodies in their diagnostic kits. See our growing line of BacTrace antibodies: • • • • • • • • • •
Anti-Salmonella Anti-Salmonella, Molecular Grade Anti-E.coli O157:H7 Anti-E.coli O157:H7, Molecular Grade Anti-Listeria, genus-specific Anti-Campylobacter, genus specific Anti-Helicobacter pylori Anti-Borrelia species Anti-Borrelia burgdorferi Anti-Renibacterium salmoninarum
BacTrace antibodies offer a range of benefits: • Sensitivity: nanogram detection of bacterial antigens within hours. • Specificity: recognize both cell surface and intracellular antigens of bacterial cells. • Versatility: ideal for screening assays in the research lab, environmental monitoring and for food and beverage analysis in the industrial setting. • Positive controls available for each BacTrace antibody. Anti-Salmonella and E.coli O157:H7, Molecular Grade New molecular grade antibodies to Salmonella and E. coli O157:H7 are recent additions to our BacTrace line. These antibodies are specially processed and QC-tested to ensure that contaminating Salmonella or E. coli DNA is not detectable by real-time PCR or other molecular methods.
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BacTrace
®
KPL has a large production capacity and offers flexible services to meet your specific needs. • • • •
Multi-gram quantities from the same purification lot Labeling options, including peroxidase, phosphatase, fluorescein Customized antibody formulations to tailor specificity and sensitivity Custom conjugation to other reporter molecules (fluorophores, enzymes, etc.)
Ordering Information Catalog#
Description
01-91-99 01-90-99-MG 05-91-99 04-91-99 02-91-99 50-74-01
Unlabeled Unlabeled, molecular grade Phosphatase-labeled Peroxidase-labeled Fluorescein-labeled Salmonella typhimurium Positive Control Antigen
Size
Anti-Salmonella (CSA) •Broadly reactive; detects 23 Salmonella species •Optimized for greatest specificity to Salmonella groups B,C,D and E
Anti-Salmonella (CSA-1) Common Structural Antigen 1.0 mg 1.0 mg 0.1 mg 0.1 mg 0.5 mg 1.0 mL
Anti-E. coli O157:H7 01-95-90 01-95-90-MG 05-95-90 04-95-90 02-95-90 50-95-90
Unlabeled Unlabeled, molecular grade Phosphatase-labeled Peroxidase-labeled Fluorescein-labeled E. coli O157:H7 Positive Control Antigen
1.0 mg 1.0 mg 0.1 mg 0.1 mg 0.5 mg 1.0 mL
Anti-Listeria, genus specific 01-90-90 05-90-90 04-90-90 02-90-90 50-90-90
Unlabeled Phosphatase-labeled Peroxidase-labeled Fluorescein-labeled Listeria, genus specific Positive Control Antigen
1.0 mg 0.1 mg 0.1 mg 0.5 mg 1.0 mL
Anti-Campylobacter, genus specific 01-92-93 05-92-93 04-92-93 02-92-93 50-92-93
Unlabeled Phosphatase-labeled Peroxidase-labeled Fluorescein-labeled Campylobacter jejuni Positive Control Antigen
1.0 mg 0.1 mg 0.1 mg 0.5 mg 1.0 mL
•Molecular grade antibody available for testing by PCR or other molecular methods. •Salmonella typhimurium positive control available
Anti-E.coli O157:H7 •Highly specific; minimal crossreactivity to non-O157 serotypes of E.coli •Molecular grade antibody available for testing by PCR or other molecular methods •E.coli O157:H7 positive control available
Anti-Borrelia burgdorferi 01-97-91 05-97-91 04-97-91 02-97-91
Unlabeled Phosphatase-labeled Peroxidase-labeled Fluorescein-labeled
1.0 mg 0.1 mg 0.1 mg 0.5 mg
Anti-Borrelia species 01-97-92 04-97-92 02-97-92 50-97-91
Unlabeled Peroxidase-labeled Fluorescein-labeled Borrelia burgdorferi Positive Control Antigen
1.0 mg 0.1 mg 0.5 mg 1.0 mL
Anti-Helicobacter pylori 01-93-94 04-93-94 02-93-94 50-93-94
Unlabeled Peroxidase-labeled Fluorescein-labeled Helicobacter pylori Positive Control Antigen
1.0 mg 0.1 mg 0.5 mg 1.0 mL
Anti-Renibacterium salmoninarum 01-96-91 05-96-91 04-96-91 02-96-91 50-96-91
Unlabeled Phosphatase-labeled Peroxidase-labeled Fluorescein-labeled R. salmoninarun Positive Control Antigen
1.0 mg 0.1 mg 0.1 mg 0.5 mg 1.0 mL
All BacTrace antibodies are made in goat.
Gaithersburg, MD 20878 Phone: 800.638.3167 Fax: 301.948.0169 www.kpl.com
ISO 9001:2000 Registered
To order or for more information on KPL’s line of protein detection products, contact us at 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com. ML366-01 For research use only. BacTrace is a registered trademark of KPL © 2008 KPL, Inc. All rights reserved.
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Peroxidase Conjugates KPL Peroxidase Conjugates: Time-tested, Sensitive and Reliable Since 1979 KPL has provided quality affinity
streptavidin. They are affinity purified and in
purified antibodies to researchers worldwide.
some cases further adsorbed to minimize cross-
Over the years we have refined our production
reactivity between animal species or shared
process to provide antibodies with high potency
reactivity with other immunoglobulin classes.
and consistent performance in immunoassays.
HRP-labeled F(ab’)2 fragment antibodies are
From the start KPL gives careful consideration
offered for assays requiring extremely low back-
to immunogen preparation, using a highly
ground and absence of F(c)-mediated binding.
purifed formulation to generate antiserum. KPL pools antiserum from multiple animals to
KPL reacts HRP of the highest quality with
reduce natural animal to animal serum variabili-
affinity purified antibodies and streptavidin
ty. During the purification process our ISO
using the periodate method of Nakane and
9001:2008-certified quality procedures impose
Kawaoi. Special features of HRP include:
rigorous standards for potency and cross-reac-
• faster catalytic rate than alkaline phosphatase • generates more product in shorter incubation
tivity. The result is standardized antibody lots with excellent reproducibility. Our extensive line of peroxidase (HRP) conjugates is available across a range of animal species, including human, mouse, rabbit and rat antibodies, as well as other animal species and
times
• provides maximum sensitivity, low nonspecific binding
• ideal for ELISA, Western blotting and immunohistology applications. SEE MORE with KPL!
Peroxidase Conjugates Peroxidase Conjugates Ordering Information (Partial listing) Catalog#
Description
Size
KPL offers a range of sensitive substrates for use with HRP conjugates. They provide a choice of intense colors for ELISA, blotting and cell staining applications.
04-10-06
HRP-labeled Goat Anti-Human IgG (H+L)
0.1 mg
04-10-17
HRP-labeled Goat Anti-Human IgA+IgG+IgM (H+L), MSA
0.1 mg
04-10-20
HRP-labeled Goat Anti-Human IgG (Fc)
0.1 mg
074-1002
HRP-labeled Goat Anti-Human IgG (γ)
1.0 mg
074-1003
HRP-labeled Goat Anti-Human IgM (μ)
1.0 mg
074-1004
HRP-labeled Goat Anti-Human IgE (ε)
1.0 mg
074-1006
HRP-labeled Goat Anti-Human IgG (H+L)
1.0 mg
074-1007
HRP-labeled Goat Anti-Human IgA+IgG+IgM (H+L)
1.0 mg
14-10-01
HRP-labeled Goat Anti-Human IgA (α)
0.5 mg
214-1002
HRP-labeled F(ab’)2 Goat Anti-Human IgG (γ)
0.5 mg
214-1003
HRP-labeled F(ab’)2 Goat Anti-Human IgM (μ)
0.5 mg
214-1006
HRP-labeled F(ab’)2 Goat Anti-Human IgG (H+L)
0.5 mg
474-1002
HRP-labeled Goat Anti-Human IgG (γ), Liquid
1.0 mL
474-1003
HRP-labeled Goat Anti-Human IgM (μ), Liquid
1.0 mL
474-1006
HRP-labeled Goat Anti-Human IgG (H+L), Liquid
1.0 mL
•
4 CN Peroxidase Substrate
04-18-06
HRP-labeled Goat Anti-Mouse IgG (H+L), HSA
0.1 mg
•
04-18-15
HRP-labeled Goat Anti-Mouse IgG (H+L), RtSA, HSA
0.1 mg
TMB Membrane Peroxidase Substrate
04-18-18
HRP-labeled Goat Anti-Mouse IgG (H+L), RbSA, HSA
0.1 mg
074-1802
HRP-labeled Goat Anti-Mouse IgG (γ), HSA
1.0 mg
074-1803
HRP-labeled Goat Anti-Mouse IgM (μ), HSA
1.0 mg
074-1806
HRP-labeled Goat Anti-Mouse IgG (H+L), HSA
1.0 mg
074-18-061
HRP-labeled Goat Anti-Mouse IgG (H+L), XSA
1.0 mg
074-1807
HRP-labeled Goat Anti-Mouse IgA+IgG+IgM (H+L), HSA
1.0 mg
074-1809
HRP-labeled Goat Anti-Mouse IgG+IgM (H+L), HSA
1.0 mg
14-18-01
HRP-labeled Goat Anti-Mouse IgA (α), HSA
0.5 mg
214-1802
HRP-labeled F(ab’)2 Goat Anti-Mouse IgG (γ), HSA
0.5 mg
214-1806
HRP-labeled F(ab’)2 Goat Anti-Mouse IgG (H+L), HSA
0.5 mg
474-1802
HRP-labeled Goat Anti-Mouse IgG (γ), HSA, Liquid
1.0 mL
474-1806
HRP-labeled Goat Anti-Mouse IgG (H+L), HSA, Liquid
1.0 mL
074-1506
HRP-labeled Goat Anti-Rabbit IgG (H+L)
1.0 mg
074-15-061
HRP-labeled Goat Anti-Rabbit IgG (H+L), XSA
1.0 mg
074-1516
HRP-labeled Goat Anti-Rabbit IgG (H+L), HSA
1.0 mg
214-1516
HRP-labeled F(ab’)2 Goat Anti-Rabbit IgG (H+L), HSA
0.5 mg
474-1506
HRP-labeled Goat Anti-Rabbit IgG (H+L), Liquid
1.0 mL
474-1516
HRP-labeled Goat Anti-Rabbit IgG (H+L), HSA, Liquid
1.0 mL
14-30-00
HRP-labeled Streptavidin
0.5 mg
Phone: 800.638.3167
474-3000
HRP-labeled Streptavidin, Liquid, Molecular Grade
1.0 mL
Fax: 301.948.0169
Visit our website at www.kpl.com for a complete listing of HRP-labeled antibodies. To order or for more information on KPL’s protein research products, contact us at 800.638.3167 / 301.948.7755, FAX 301.948.0169 or visit us at www.kpl.com.
ELISA •
ABTS® 1- and 2-Component Microwell Peroxidase Substrates
•
SureBlueTM TMB Peroxidase Substrate
•
SureBlue ReserveTM TMB Peroxidase Substrate
•
TMB Peroxidase Substrate
Blotting
•
•
LumiGLO®Chemiluminescent Substrate LumiGLO ReserveTM Chemiluminescent Substrate
Whichever substrate you choose, enjoy the benefits of excellent signalto-noise and reproducibility.
Gaithersburg, MD 20878
www.kpl.com
ISO 9001:2008 Registered
For research use only. ©2009 KPL, Inc. All rights reserved. ABTS is a registered trademark of Roche Biochemicals. ML371-01
SEE MORE with KPL!
Ordering Information
Catalog #
Description
Size
Catalog #
Description
Size
Protein Detector ELISA Kits
Phosphatase Chemiluminescent Substrates
Each kit includes an AP- or HRP-labeled Conjugate, Coating Solution, Wash Solution Concentrate, 10% BSA Diluent/Blocking Solution Concentrate, Stop Solution and Substrate.
55-60-03
PhosphaGLO AP Substrate
55-60-04
Same as above
55-60-01
PhosphaGLO Reserve AP Substrate
Phosphatase
55-60-02
Same as above
30 mL 100 mL 30 mL 100 mL
55-81-10
AP ELISA Kit, Anti-Human
20 plates
55-81-50
AP ELISA Kit, Anti-Rabbit and Anti-Mouse
20 plates
54-62-10
HRP ELISA Kit, Anti-Human
20 plates
All antibodies listed below are produced in goat. For a complete antibody listing, visit www.kpl.com or refer to KPL's Product Catalog.
54-62-18
HRP ELISA Kit, Anti-Mouse
20 plates
Phosphatase
54-62-15
HRP ELISA Kit, Anti-Rabbit
20 plates
075-1006
ReserveAP Anti-Human IgG (H+L)
1.0 mg
075-1806
ReserveAP Anti-Mouse IgG (H+L), HSA
1.0 mg
075-1506
ReserveAP Anti-Rabbit IgG (H+L)
1.0 mg
Antibody Conjugates
Peroxidase
Substrates for ELISA Phosphatase Chromogenic Substrates 50-88-02
BluePhos Microwell Substrate Kit
50 mL
50-88-00
Same as above
600 mL
074-1006
Anti-Human IgG (H+L)
1.0 mg
50-88-01
Same as above
2.7 L
074-1806
Anti-Mouse IgG (H+L), HSA
1.0 mg
50-87-10
FirePhos Microwell Substrate Kit
50 mL
074-1506
Anti-Rabbit IgG (H+L)
1.0 mg
50-87-20
Same as above
200 mL
Biotin-Labeled
50-87-30
Same as above
600 mL
16-10-06
Anti-Human IgG (H+L)
0.5 mg
50-87-40
Same as above
2.7 L
16-18-06
Anti-Mouse IgG (H+L), HSA
0.5 mg
50-80-00
pNPP Phosphatase Substrate System
500 mL
16-15-06
Anti-Rabbit IgG (H+L)
0.5 mg
50-80-01
pNPP Phosphatase Tablets
100 tablets
Peroxidase
Labeled Streptavidin
Peroxidase Chromogenic Substrates
15-30-00
AP-labeled
0.5 mg
52-00-01
14-30-00
HRP-labeled
0.5 mg
SureBlue TMB 1-Component Substrate
100 mL
52-00-02
Same as above
400 mL
52-00-03
Same as above
1L
53-00-01
SureBlue Reserve TMB 1-Component Substrate
100 mL
Blocking Solutions
53-00-02
Same as above
400 mL
50-61-00
10% BSA Diluent/Blocking Solution
200 mL
1L
50-61-10
10% BSA Diluent/Blocking Solution
1L
600 mL
50-82-01
Milk Diluent/Blocking Solution
53-00-03
Same as above
50-76-00
TMB 2-Component Substrate Kit
50-84-00
Coating Solution Concentrate
50-63-00
Wash Solution Concentrate
50 mL 800 mL
200 mL
Conjugate Stabilizers
50-76-03
Same as above
50-66-18
ABTS Microwell Substrate 1-Component
55-15-00
APStabilizer
200 mL
100 mL
54-15-01
HRPStabilizer
200 mL
50-66-00
Same as above
600 mL
Stop Solutions
50-66-06
Same as above
1L
50-85-01
ABTS Stop Solution
200 mL
50-62-00
ABTS Substrate System 2-Component
50-89-00
APstop Solution
200 mL
50-85-05
TMB Stop Solution
400 mL
50-85-06
TMB Stop Solution
50-85-30
TMB BlueSTOP Solution
400 mL
50-85-41
TMB BlueSTOP Solution
1L
50-62-01
Same as above
2.7 L
Assay Support Reagents
600 mL 2700 mL
Peroxidase Chemiluminescent Substrates 54-61-00
LumiGLO Chemiluminescent Substrate Kit
240 mL
54-61-01
Same as above
720 mL
54-61-02
Same as above
60 mL
54-71-00
LumiGLO Reserve Chemiluminescent Substrate Kit
54-71-01
ML299-03
Same as above
1L
2400 cm2 600 cm2
HSA: Human serum adsorbed
To order, call 800.638.3167 or 301.948.7755, fax 301.948.0169, online at www.kpl.com or contact your local KPL Sales Partner. ISO 9001:2000 Registered
SEE MORE with KPL!