io B tech r o f s t Optical Producstrumentation & Analytical In
Fluorescence Filters Edge Filters Narrowband Laser-line Filters Notch Filters High-Performance Mirror Lasers Optical Instrumentation Optics & Optomechanics
Bulletin No. 3011
www.laser2000.de
What’s Inside
Filters Made with Only Hard Coatings
NEW – UV Optical Filters!
Raman filters Laser-line filters
MaxLine®
Laser-diode filters
MaxDiode™
Notch filters
StopLine®
Laser mirror
MaxMirror®
Clinical Filter Sets . . . . . . . . . .22 Bandpass Filters . . . . . . . . . . .24 Dichroic Beamsplitters (45°) .28
RazorEdge®
λ
Single-band Filter Sets . .6 Multiband Filter Sets . . . .6 UV Filters . . . . . . . . . . . .10 Multiphoton Filters . . . .20
BrightLine®
For the fastest fluorescence measurements and “no burn-out”
Fluorescence filters
Fluorescence Filters and Sets . . . . . . . . . . . . . . . .4
T
Raman Edge Filters . . . . . . . . . . . . . . . . . . . . . . .32
T
Razor sharp – for the most discriminating Raman measurements 248 nm – 1064 nm
λ
Laser-line Filters . . . . . . . . . . . . . . . . . . . . . . . . . .36
T
Clean up your laser with ultra-fast roll-off and the highest peak transmission – don’t waste expensive laser light! λ
266 nm – 1064 nm
Laser Diode Clean-up Filters . . . . . . . . . . . . . . .41
T
High transmission, low ripple, and exceptional noise blocking for the cleanest diode laser output λ
375 nm – 635 nm
Laser Notch Filters . . . . . . . . . . . . . . . . . . . . . . .42
T
Deep Laser-blocking Filters Single-notch Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42 Multi-notch Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
λ
405 nm – 830 nm
Ultra-broadband High-performance Mirror . . .47
R
The most versatile high-performance mirror on the market – there is nothing else like it! λ
350 nm – 1100 nm in just one mirror
For more innovative filters and pioneering OEM solutions, see www.semrock.com.
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Lasers
Optics & Optomechanics
Femtosecond Lasers.............................................................................49
Optics and Optomechanics..................................................................60
Diode pumped solid state lasers.........................................................50
Laser Safety Goggles............................................................................60
Deep UV Gas Lasers..............................................................................52 Digital Detector Controllers..................................................................53 Spectrally Programmable Light Engine..............................................58
Service Free Catalogue Order............................................................................61
Optical Instrumentation Silicon Solutions for Low Light Detection..........................................54 Spectroscopy Cameras.........................................................................54 NIR InGaAs Spectrometers..................................................................56 UV-VIS Spectrometers..........................................................................57
Welcome to the latest LASER 2000 catalogue for Biotechnology and Analytical Instrumentation... In this issue we focus on the industry-leading filters from Semrock, which are brighter and spectrally more sophisticated than any other filter. In addition we give you a short selection of other superior and very useful products for these applications like lasers, sensors, optics and optomechanics.
When dealing with Laser 2000, you will receive advice and information from trained engineers and physicists, whose technical expertise is of the highest level. With products in service at thousands of locations all over the world, you can be sure that LASER 2000 will provide you with the performance and reliability that you need. Contact us today to find out how you can benefit from our technology.
Product Specialists Bernhard Dauner
Dr. Georg Draude
Mark Drechsler
+49 (0) 8153-405-17 b.dauner@laser2000.de
+49 8153 405-83 g.draude@laser2000.de
+49 (0) 8153-405-54 m.drechsler@laser2000.de
Dr. Stefan Kremser
Dr. Helge Br端ggemann
+49 (0) 8153-405-16 s.kremser@laser2000.de
+49 (0) 30 962 778-12 h.brueggemann@laser2000.de
Product Specialist
Product Specialist
Product Specialist
Product Specialist
Product Specialist
Sales Assistance Victoria Benedikt
Gabriela Thunig
Anette Hartl
+49 (0) 8153-405-61 v.benedikt@laser2000.de
+49 (0) 8153-405-43 g.thunig@laser2000.de
+49 8153 405-58 a.hartl@laser2000.de
Sales Assistance
Sales Assistance
www.laser2000.de
Sales Assistance
Introduction Semrock Hard-coated Ion Beam Sputtered Optical Filters The Standard in Optical Filters for Biotech & Analytical Instrumentation
Transmission (%)
Semrock introduced a revolutionary optical filter technology to Biotech and Analytical Instrumentation. For more than six years, Semrock has been successfully combining the most sophisticated and modern ion-beam sputtering deposition systems, renowned for their stability, with its own proprietary deposition control technology, unique predictive algorithms, process Four different batches; identical results! improvements and volume manufacturing capability. The result is 100 optical filters of unsurpassed performance and reliability that have 90 80 set the standard for the biotech and analytical instrumentation 70 industries.These filters are so exceptional that they are award60 winning and so innovative that they are patented. And Semrock 50 never stops improving. 40 That is why Semrock consistently delivers:
Batch 1 Batch 2 Batch 3 Batch 4
30 20
The brightest filters with the most discriminating spectra for the fastest measurements
10 0
400
440
Proven reliability for permanent performance; see page 3
480
520
560
600
Wavelength (nm)
Unsurpassed batch-to-batch reproducibility for assured supply
We are your Biotech & Analytical Instrumention Optics Experts Semrock knows optics! We routinely do optical ray tracing, run Monte Carlo simulations, perform stray light analysis, and of course craft sophisticated optical filter designs. Why is all this necessary? We believe that in order to design the best optical filters, great filter technology is not enough – we must also thoroughly understand your applications. And because we have long-focused all of our resources on the biotech and analytical instrument markets, we have a profound understanding of our customers’ needs. We thoroughly understand optics, we bring you great filters, and we also speak your language!
Product lines: Fluorescence Filters Fluorescence microscopy, DNA microarray scanners, high-throughput and high-content screening, flow cytometry, real-time PCR, microplate readers, electrophoresis scanners, fluorescence imagers, chemical process monitoring... Raman Spectroscopy Filters Raman microscopes, laboratory spectrometers, Raman microprobe analyzers (including optical fiber probes)... Biomedical Laser Optics Ophthalmological, dermatological, and other medical laser systems, laser-based scanners, multiphoton fluorescence systems and microscopes...
Product Specialists Bernhard Dauner +49 (0) 8153-405-17 b.dauner@laser2000.de
Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
Call us: +49 (0)8153 405-0
Introduction Semrock Hard-coated Ion Beam Sputtered Optical Filters Proven Reliability All Semrock filters demonstrate exceptional reliability. The simple all-glass structure and hard dielectric glass coatings (as hard as the glass on which they are coated!) mean they are virtually impervious to humidity-induced shifting and can be cleaned and handled like any standard glass optics. All Semrock coatings are hard – never soft. All Semrock filters are capable of withstanding high optical intensities. Semrock filters either have laser-damage threshold specifications (see page 46) or, depending on the application, have been tested with intense broadband sources for prolonged periods of time with no noticeable degradation. Semrock filters do not burn out under normal conditions, even with prolonged use and with ultraviolet (UV) irradiation. You will never find any adhesives in the optical path of any Semrock filter. Epoxies and cements can absorb water vapor and swell or shift, and they can photo-darken or be optically damaged. Semrock filters are built for longevity. Semrock filters have been tested to meet or exceed requirements for environmental and physical durability set forth in specifications such as MIL-STD-810F, MIL-C-48497A, MIL-C-675C, and ISO 9022-2. The table below shows some of the key standards against which our filters are regularly tested. Environmental Durability Testing
Standard / Procedure
Test Description
Humidity High Temperature Low Temperature
MIL-STD-810F (507.4) MIL-STD-810F (501.4) MIL-STD-810F (502.4)
Aggravated Humidity (> 10 x 24 hr cycles) Induced Hot (> 7 x 24 hr cycles) Cold (C2) (24 hr cycles)
Physical Durability Testing
Standard / Procedure
Test Description
Adhesion Humidity Moderate Abrasion Solubility/Cleanability Water Solubility
MIL-C-48497A (4.5.3.1) MIL-C-48497A (4.5.3.2) MIL-C-48497A (4.5.3.3) MIL-C-48497A (4.5.4.2) MIL-C-48497A (4.5.5.3)
“Tape Test” Damp Heat “Cheesecloth Test” (> 50 cycles) Sequential immersion in acetone and alcohol Immersion in distilled water (> 24 hrs)
Semrock’s dedicated reliability laboratory with state-of-the-art environmental test chambers is in regular use.
Example of bandpass filter spectra measured before and after ten 24-hour cycles of Aggravated Humidity testing according to MIL-STD-810F, demonstrating negligible change.
No measurable change! 100 90
Transmission (%)
80 70 60 50 Before After
40 30 20 10 0 400
420
440
460
480
500
Wavelength (nm)
RoHS Compliance: Semrock products are compliant, without exemptions, with EU Directive 2002/96/EC, Restrictive Use of Hazardous Substances (RoHS). Semrock has instituted controls to ensure our suppliers provide only RoHS compliant materials for our products.
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Fluorescence Filters BrightLine® Fluorescence Filters When You Want the Best! The award-winning BrightLine series of optical fluorescence filters has revolutionized fluorescence instruments – from microscopes to microarrays. That’s why leading OEMs around the world have designed BrightLine filters into their fluorescence systems. Semrock adapted and improved the premier optical coating technology in the industry, Ion Beam Sputtering (IBS), to solve the toughest fluorescence problems. By combining this state-of-the-art technology with our deep understanding of fluorescence applications we have produced the brightest, most durable fluorescence filters available anywhere. In fact BrightLine filter technology is so innovative that it is patented (U.S. Patent No. 6,809,859 and pending).
Spectacular Spectra Typical GFP-3035B Filter Set for Green Fluorescent Protein 100
BrightLine filters are perfectly optimized for fluorescence applications. Semrock’s state-of-the-art ion-beam sputtering technology combined with its patented single-substrate filter construction result in the highest transmission on the market. And steeper edges, precise wavelength accuracy and carefully optimized blocking mean better contrast and faster measurements – plus affordable “ZERO pixel shift” performance is available from stock!
90
Transmission (%)
80 Exciter Dichroic Emitter
70 60 50 40
Emission Spectrum
Absorption Spectrum
30 20 10 0 400
450
500
550
600
Wavelength (nm)
BrightLine Filters are Bright “I got the filters, but you forgot to include the sunglasses! These are the brightest filters I have ever seen!! Kudos.” Michael W. Davidson Molecular Expressions™ National High Magnetic Field Laboratory The Florida State University
Photos courtesy Mike Davidson at Molecular Expressions, using BrightLine® fluorescence filter sets.
The Standard in Optical Filters for Biotech & Analytical Instrumentation > 100,000 Ion Beam Sputtered filters delivered – extensive inventory now!
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Fluorescence • New ultraviolet (UV) fluorescence filters (see pages 6 Filters
efficiently access wavelengths below 300 nm!
BrightLine® Fluorescence Filters
BrightLine Durability (see page 3) • Proven Amazing multiband filters sets (see page 15) – see wha you’ve been missing! Product Specialists
Sales Assistance
X Impervious to humidity, insensitive to temperature
Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
X No soft coatings – no exceptions
BrightLine®
X Can be cleaned and handled, even with acetone!
Fluorescence filters 100
Bernhard Dauner +49 (0) 8153-405-17 b.dauner@laser2000.de
All BrightLine filters: hard dense glass coatings on hard glass substrates
• Multiphoton filters (see page 20) that will astonish eve All BrightLine filters: no “burn-out,” no annual replacement
X Stand up to intense Xenon and Mercury arc-lamp and halogen light sources X No adhesives in the optical path to darken or degrade X Made with the same refractory materials as our high “laser damage threshold” laser optics
Superior Clinical Offerings Cutting-edge Research Offerings
Semrock is continuously improving its already cutting edge Ion Beam Sputtering technology, regularly introducing new state-of-the-art filters for the most discriminating researcher.
• New ultraviolet (UV) fluorescence filters (see pages 6 & 10) – efficiently access wavelengths below 300 nm! • Amazing multiband filters sets (see page 15) – see what you’ve been missing! • Multiphoton filters (see page 20) that will astonish even the most discerning researchers
Superior Clinical Offerings 100 90
60
100
90
50 80
Transmission (%)
Transmission (%)
80 70
70 60
40 50
BrightLine FF01-280/20 UV filter Traditional metal-dielectric filter
40
30 30
BrightLine FF01-280/20 UV filter Traditional metal-dielectric filter
20 10
20 0 200
300
400
500
600
700
Wavelength (nm)
10 0 200
300
400
500
Wavelength (nm)
600
700
With Ion Beam Sputtering manufacturing prowess second-to-none, and after years of continuous improvement, Semrock recently introduced the famous durability and consistency of its hard-coated BrightLine technology for the mainstream clinical market. Our spectacular BrightLine Clinical™ series of fluorescence filters (see page 22) are optimized specifically for the costsensitive clinical user. In field trials by independent clinical laboratory personnel, BrightLine Clinical filter sets were found to have significantly better performance than even the best conventional soft-coated filter sets, yet BrightLine Clinical filters do not burn out or degrade with use, and are fully cost-competitive!
BrightLine Clinical™ Fluorescence Filter Sets Hard-coated performance at a soft-coated price™
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5
Fluorescence Filters BrightLine® Fluorescence Filters Research Microscopy Filter Sets (For stunning, yet affordable, Clinical Microscopy Filter Sets, see page 22.) Semrock carries a broad range of high-performance no “burn-out” fluorescence microscopy filter sets that will satisfy even the most demanding researcher. Single-band sets are available with an affordable “zero pixel shift” option that is ideal for making precise multi-color composite images (see page 13). Or choose from our amazing multiband filter sets for simultaneous and high-speed sequential imaging of samples with multiple fluorophores (see page 15).
Emission Color
Full Set Prefix
Type of Set [1]
Primary Fluorophores [2]
TRP-A-
Single-band
Tryptophan – New UV set
10
DAPI-1160A-
Single-band
DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350 – Highest contrast set
10
Page
Single-band Filter Sets NEW!
DAPI-5060B-
Single-band
DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350 – Highest brightness set
10
CFP-2432A-
Single-band
CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1
10
FURA2-B-
Single-band
Fura-2 Ca2+ indicator, LysoSensor Yellow/Blue
11
GFP-3035B-
Single-band
GFP, EGFP, DiO, Cy2, YOYO-1, YO-PRO-1
11
FITC-3540B-
Single-band
FITC, rsGFP, Bodipy, FAM, Fluo-4, Alexa Fluor 488
11
YFP-2427A-
Single-band
YFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123
11
TRITC-A-
Single-band
TRITC, Rhodamine, DiI, 5-TAMRA, Alexa Fluor 532
12
Cy3-4040B-
Single-band
Cy3, DsRed, PE, TAMRA, Calcium Orange, Alexa Fluor 546 & 555
12
TXRED-4040B-
Single-band
Texas Red®, Cy3.5, 5-ROX, Mitotracker Red, Alexa Fluor 568 & 594
12
Cy5-4040A-
Single-band
Cy5™, APC, DiD, Alexa Fluor 647 & 660
12
“Full Multiband”
Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 Yellow: YFP, Calcium Green-1, Eosin, Rhodamine 123
16
Green: GFP, rsGFP, FITC, Alexa Fluor 488 Red: DsRed, TRITC, Cy3, Texas Red®, Alexa Fluor 568 & 594
17
Dual-band Filter Sets CFP/YFP-A-
NEW!
CFP/YFP-2X-A-
“Pinkel”
CFP/YFP-2X2M-A-
“Sedat”
GFP/DsRed-2X-A-
“Pinkel”
GFP/DsRed-2X2M-B-
“Sedat”
GFP/HcRed-2X-A-
“Pinkel”
Green: GFP, rsGFP, FITC, Alexa Fluor 488 Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594
17
BFP/GFP/HcRed-3X-A-
“Pinkel”
Blue: BFP, DAPI, Hoechst, AMCA, Alexa Fluor 350 Green: GFP, rsGFP, FITC, Alexa Fluor 488 Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594
17
CFP/YFP/HcRed-3X-A-
“Pinkel”
16
CFP/YFP/HcRed-3X3M-A-
“Sedat”
Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 Yellow: YFP, Calcium Green-1, Fluo-3, Rhodamine 123 Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594
DA/FI/TX-B-
“Full Multiband”
15
DA/FI/TX-3X-A-
“Pinkel”
DA/FI/TX-3X3M-A-
“Sedat”
Blue: DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350 Green: FITC, GFP, rsGFP, Bodipy, Alexa Fluor 488 Red: Texas Red®, MitoTracker Red, Alexa Fluor 568 & 594
Blue: DAPI, Hoechst, AMCA, Alexa Fluor 350 Green: FITC, GFP, rsGFP, BODIPY, Alexa Fluor 488 Orange: TRITC, Cy3, Texas Red®, MitoTracker Red, Alexa Fluor 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor 647 & 660
18
Triple-band Filter Sets
Quadruple-band Filter Sets DA/FI/TR/Cy5-4X-A-
“Pinkel”
DA/FI/TR/Cy5-4X4M-B-
“Sedat”
[1]
Single-band Set consists of a single-band exciter, single-band emitter, and single-edge dichroic beamsplitter; “Full Multiband” Set consists of multiband exciter, multiband emitter, and multiband dichroic beamsplitter; “Pinkel” Set consists of single-band exciters, multiband emitter and multiband dichroic beamsplitter; “Sedat” Set consists of single-band exciters, single-band emitters, and multiband dichroic beamsplitter. See the Technical Note on page 19 for more information.
[2]
For a complete list of fluorophores and filter compatibility, go to www.semrock.com.
All in Stock – Only at Semrock!
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Fluorescence Filters BrightLine® Fluorescence Filters Semrock Cube Designation
Part Number for Cube
Part Number [1] for Filter Set Mounted in Cube
TE2000
NTE
<Full set prefix>-NTE
Quadfluor
NQF
<Full set prefix>-NQF
Nikon TE2000, 80i, 90i, 50i, and any using the Epi-fluor Illuminator E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, ME600L, L150A, and some Labophot, Optiphot, and Diaphot series
BrightLine®
Microscope Brand/ Compatible Microscopes
Fluorescence filters 100
Microscope Cubes and Sets Mounted in Cubes (also known as holders)
Olympus AX, BX, and IX series MVX10 (and other large optical path microscopes)
U-MF2
OMF
<Full set prefix>-OMF
U-MF/XL
Contact Semrock
Contact Semrock
FL CUBE EC P&C
ZHE
<Full set prefix>-ZHE
ZAT
<Full set prefix>-ZAT
<Full set prefix>-LDMK-ZERO
Zeiss Axio Imager, Axioplan2i, Axioplan2ie, Axiovert200, and Axioskop2 (post-2001) Axioplan (pre-version 2), Axiovert100, and Axioskop2 (pre-2002)
(Push-and-Click)
Threaded Filter Cube
Leica DM-4000, DM-5000, and DM-6000
DM-K
LDMK
DM-LB, DM-LM, DM-LP, DM-RB, and DM-R HCRF4
DM-R
LLC
<Full set prefix>-LLC-ZERO
DM-IRB
LSC
<Full set prefix>-LSC-ZERO
DM-IL, DM-IRB, DM-LS, DM-LSP, DM-R HCRF8, and DM-R XARF8 [1]
See page 6 and page 23 for <Full set prefix>.
Nikon, NTE
Nikon, NQF
Olympus, OMF
Zeiss, ZHE
Zeiss, ZAT
Leica, LDMK
Leica, LLC
Leica, LSC
For a nominal amount more you can experience certified “zero-pixel-shift” performance. BrightLine ZERO™ single-band fluorescence filter sets are affordable and in stock. Just append –ZERO. For example,TRITC-A-NTEZERO is the TRITC set in “ZERO” form mounted in a Nikon TE2000 cube. Leica single-band sets are only available as –ZERO sets. See page 13 for more information on BrightLine ZERO™ filters.
Product Specialists Bernhard Dauner +49 (0) 8153-405-17 b.dauner@laser2000.de
Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
www.semrock.com
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7
Fluorescence Filters BrightLine® Fluorescence Filters Common Specifications (for Single and Multiband Sets) Property
Exciter
Emitter
Dichroic
Comment
> 93% > 97% N/A 0° ± 5°
> 90% > 93% > 98% 45° ± 1.5°
Averaged over passband (emitter band only for dichroic)
Dichroic Reflection Angle of Incidence
> 93% > 97% N/A 0° ± 5°
Cone Half Angle [2] Autofluorescence Transverse Dimensions
7° Low 25.0 mm
7° Low 25.0 mm
2° Ultra-low 25.2 x 35.6 mm
Transverse Dimension Tolerance Thickness Thickness Tolerance Clear Aperture
+ 0.0 / – 0.1 mm 5.0 mm 3.5 mm ± 0.1 mm > 21 mm > 22 mm
± 0.1 mm 1.05 mm ± 0.05 mm > 80%
Passband Transmission [1]
Guaranteed Typical
Edge Chipping Ring Housing Material
< 0.1 mm Aluminum, black-anodized
Surface Quality Coating Type Blocking
Filter Orientation Microscope Compatibility [2]
Filter sizes shown are standard for most Nikon, Olympus, and Zeiss microscopes. For Leica , see www.semrock.com. FF01-357/44-25 is 5.0 mm thick Area over which all optical specifications are met Measured from substrate edge Exciter and emitter only; Leica filters are not mounted in aluminum rings Measured within clear aperture
60-40 scratch-dig “Hard” ion-beam-sputtered BrightLine filters have blocking far exceeding OD 6 wherever needed to ensure the blackest background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ filter design software. Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrate construction for unrivaled filter life. BrightLine filters are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards; see page 3 for details Arrow on ring indicates direction of propagation of light; see diagram below BrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes.
Reliability and Durability
[1]
Averaged over exciter band(s) Range of angles over which optical specifications are guaranteed for collimated light For uniformly distributed non-collimated light
For transmission specifications for UV and multiband filters, go to www.semrock.com. Filter performance is likely to remain satisfactory for Cone Half Angles as large as 10° for exciters and emitters, and 3° for dichroics.
T E C H N I C A L N OT E Orientation of Filters in a Microscope Because BrightLine filters are so durable, you can easily populate your own cubes, sliders, and filter wheels without having to worry about damaging the filters. To obtain the most optimal performance from the filters, they should be oriented properly. The diagrams below explain the proper orientation.
emitter dichroic
to eye or camera BrightLine®
BrightLine®
exciter
light source sample
reflective coating side
The exciter and emitter should be oriented so that the arrow on the side of the aluminum ring points in the direction of propagation of the desired light – from the light source to dichroic for the exciter and from the dichroic to eye or camera for the emitter.The dichroic must be oriented such that the reflective coating side faces toward the exciter or light source and the sample.
reflective coating side
reflective coating side
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When viewing the dichroic with the reflective coating side down, you can see a doublereflection of a bright object and the thickness of the filter at the far edge is apparent. When viewing the dichroic with the reflective coating side up, you can see a predominantly single reflection of a bright object and the thickness of the filter at the far edge is not visible.
Fluorescence Filters BrightLine® Fluorescence Filters
detector
Optical fluorescence occurs when a molecule absorbs light at wavelengths within its absorption band, and then nearly instantaneously emits light at longer wavelengths within its emission band. For analytical purposes, strongly fluorescing molecules known as fluorophores are specifically attached to biological molecules and other targets of interest to enable quantification, identification, and even real-time observation of biological and chemical activity. Fluorescence is widely used in biotechnology and analytical applications due to its extraordinary sensitivity, high specificity, and simplicity.
BrightLine®
Introduction to Fluorescence filters
Fluorescence filters 100
T E C H N I C A L N OT E
Most fluorescence instruments, including fluorescence microscopes, are based on optical filters. A typical system has three basic filters: an excitation filter (or exciter), a dichroic beamsplitter, and an emission filter (or emitter). The exciter is typically a bandpass filter that passes only the wavelengths absorbed by the fluorophore, emission filter dichroic beamsplitter thus minimizing excitation of other sources of fluorescence and blocking light in the fluorescence excitation filter emission band.The dichroic is an edge filter used at an oblique angle of incidence (typically 45°) to efficiently reflect light in the excitation band and to transmit light in the emission band.The emitter is typically a bandpass filter that passes only the wavelengths emitted by the fluorophore and blocks all undesired light outside this band – especially the excitation light. broadband light source
Filter Transmission
dichroic beamsplitter excitation filter
emission filter
sample absorption
sample fluorescence
Wavelength of Light
In most fluorescence instruments, the best performance is obtained with thin-film filters, which sample are comprised of multiple alternating thin layers of transparent materials with different indexes of refraction on a glass substrate.The complex layer structure determines the spectrum of light transmission by a filter.Thin-film filters are simple to use, inexpensive, and provide excellent optical performance: high transmission over an arbitrarily determined bandwidth, steep edges, and high blocking of undesired light over the widest possible wavelength range. Recent advances in thin-film filter technology, unique to BrightLine filters, permit even higher performance while resolving the longevity and handling issues that can affect filters made with older soft-coating technology.
T E C H N I C A L N OT E 100
Ultraviolet (UV) Fluorescence Applications
90 80
Transmission (%)
Many biological molecules of interest naturally fluoresce when excited by shorterwavelength UV light. This “intrinsic fluorescence” can be a powerful tool. One important application is the direct fluorescence imaging of aromatic amino acids including tryptophan, tyrosine, and phenylalanine, which are building blocks for proteins. The aromatic rings in these molecules give rise to strong fluorescence excitation peaks in the 260 to 280 nm range. Because the fluorescence is intrinsic, samples can be observed without the added chemistry and limitations associated with “indirect” labeling by extrinsic fluorophores. Another important application is DNA quantitation. Purines and pyrimidines – bases for nucleic acids like DNA and RNA – have strong absorption bands in the 260 to 280 nm range.The neurotransmitter serotonin also exhibits strong absorption at these wavelengths and associated intrinsic fluorescence.
70 60 50 BrightLine FF01-280/20 UV filter Traditional metal-dielectric filter
40 30 20 10 0 200
300
400
500
700
600
Wavelength (nm)
New BrightLine FF01-280/20-25 filter (see page 24).
100 90 Exciter Dichroic Emitter
80
Transmission (%)
Semrock’s UV BrightLine fluorescence filters offer a new and powerful tool for practicing direct fluorescence imaging. These new UV filters are both reliable (no “burn-out”) and offer performance nearly comparable to that of visible and near-IR filters. The top figure shows the spectrum of a high-reliability 280 nm BrightLine excitation filter with the highest commercially available transmission (> 70%), remarkably steep edges, and wideband blocking across the entire UV and visible spectrum. This spectrum is directly compared to that of a traditional and inferior metal-dielectric filter. In one example system this filter difference was shown to provide well over 100x improvement in signal-to-noise ratio. The bottom figure shows the spectra from a new UV filter set designed for imaging tryptophan, overlaid on the absorption and emission spectra of that amino acid. Note the nearly ideal overlap and high transmission of all three filters in this set.
70 60 50 40 30 20 10 0 250
275
300
325
350
375
400
425
450
Wavelength (nm)
TRP-2044A single-band fluorescence filter set is ideal for imaging tryptophan (see page 10).
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9
Fluorescence Filters BrightLine® Fluorescence Filters Single-band Fluorescence Filter Sets – Made With ONLY Hard Coatings DAPI-1160A – Highest Contrast
TRP-A Exciter Part Number . . . FF01-280/20-25 Emitter Part Number. . . FF01-357/44-25-5.0 Dichroic Part Number. . FF310-Di01-25x36 Full Set Prefix . . . . . . . . . TRP-A-_ _ _
NEW!
Exciter Part Number . . .FF01-387/11-25 Emitter Part Number . . .FF02-447/60-25 Dichroic Part Number . .FF409-Di02-25x36 Full Set Prefix . . . . . . . . .DAPI-1160A-_ _ _
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CFP-2432A
Exciter Part Number . . .FF01-377/50-25 Emitter Part Number . . .FF02-447/60-25 Dichroic Part Number . .FF409-Di02-25x36 Full Set Prefix . . . . . . . . .DAPI-5060B-_ _ _
Exciter Part Number . . .FF01-438/24-25 Emitter Part Number . . .FF01-483/32-25 Dichroic Part Number . .FF458-Di01-25x36 Full Set Prefix . . . . . . . . .CFP-2432A-_ _ _ 100
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Wavelength (nm) Brightest DAPI set on the market. This filter set is recommended for those special cases in which it is critical to achieve the highest possible brightness for the shortest possible exposure times, or for very low fluorophore concentrations. For shorter exposure times, the set exhibits up to 5 times more brightness, but has lower contrast than the DAPI-1160A set.
This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) filter set available for measuring CFP and its variants – no more compromising or exchanging filter sets for different CFP experiments. Filters from this set are ideal for CFPYFP FRET measurements when used in conjunction with filters from our YFP-2427A filter set (see page 11 and page 14).
See page 6 for a list of primary fluorophores. Go to www.semrock.com for ASCII data and a complete fluorophore listing.
10
450
Highest Contrast DAPI set on the market. The preferred DAPI set for most applications, this filter set is optimized for achieving very low noise while maintaining excellent brightness, resulting in unsurpassed contrast. The signal-to-background ratio achieved with this DAPI set for imaging typical nuclear-stain samples is more than twice that of a leading competitor’s.
DAPI-5060B – Highest Brightness
Transmission (%)
400
Wavelength (nm)
New UV fluorescence filter set! Only Semrock offers true UV fluorescence filters with high transmission, edge steepness, and blocking that rival high-performance visible filters. The filters in this set are ideal for imaging the intrinsic fluorescence from aromatic amino acids (like tryptophan) and other UV-excited fluorescent species. See page 9 for a Technical Note on UV Fluorescence.
0 300
Dichroic
Emitter
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20
0 250
Exciter
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Emitter
Transmission (%)
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Dichroic
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Fluorescence Filters BrightLine® Fluorescence Filters Single-band Fluorescence Filter Sets – Highest Performance & Durability
Exciter Part Number . . .FF01-340/26-25 Exciter Part Number . . .FF01-387/11-25 Emitter Part Number . . .FF01-510/84-25 Dichroic Part Number . .FF409-Di02-25x36 Full Set Prefix . . . . . . . . .FURA2-B-_ _ _
Exciter Part Number . . .FF01-472/30-25 Emitter Part Number . . .FF01-520/35-25 Dichroic Part Number . .FF495-Di02-25x36 Full Set Prefix . . . . . . . . .GFP-3035B-_ _ _ 100
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Exciter 1 (saturated)
50 40
Exciter 2 (free)
30
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YFP-2427A
Exciter Part Number . . .FF01-482/35-25 Emitter Part Number . . .FF01-536/40-25 Dichroic Part Number . .FF506-Di02-25x36 Full Set Prefix . . . . . . . . .FITC-3540B-_ _ _
Exciter Part Number . . . FF01-500/24-25 Emitter Part Number. . . FF01-542/27-25 Dichroic Part Number. . FF520-Di01-25x36 Full Set Prefix . . . . . . . . . YFP-2427A-_ _ _ 100
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550
This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) filter set available for measuring GFP and its variants – no more compromising or exchanging filter sets for different GFP experiments. Filters from this set are excellent for GFP-DsRed FRET measurements when used in conjunction with the FF01-593/40 filter. See page 14 for a description of FRET.
FITC-3540B
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This four-filter set achieves high-performance ratiometric imaging of Ca2+ using Fura-2 as an indicator. The carefully optimized excitation filters provide the best balance of free and saturated Ca2+ signals while minimizing the crosstalk and noise, even when using light sources of relatively limited ultraviolet output. See Technical Note on page 14.
80
Dichroic
Emitter
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Exciter
BrightLine®
Fluorescence filters 100
GFP-3035B
FURA2-B
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Wavelength (nm)
Wavelength (nm) This set is optimized for the most popular green fluorophores like FITC (fluorescein isothiocyanate) and FAM. It provides the highest brightness and signal-to-noise ratio. It is also ideal for similar fluorophores but with narrower, more closely spaced excitation and emission peaks, like Bodipy. For FITC filter sets optimized for clinical applications, see page 23.
This filter set is optimized to be simultaneously the brightest and the highest signal-to-noise ratio (contrast) filter set available for measuring YFP and its variants – no more compromising or exchanging filter sets for different YFP experiments. Filters from this set are ideal for CFP-YFP FRET measurements when used in conjunction with filters from our CFP-2432A filter set (see page 10 and page 14).
See page 6 for a list of primary fluorophores. Go to www.semrock.com for ASCII data and a complete fluorophore listing.
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11
Fluorescence Filters BrightLine® Fluorescence Filters Single-band Fluorescence Filter Sets – Highest Performance & Durability TRITC-A
Cy3-4040B
Exciter Part Number . . . FF01-543/22-25 Emitter Part Number. . . FF01-593/40-25 Dichroic Part Number. . FF562-Di02-25x36 Full Set Prefix . . . . . . . . . TRITC-A-_ _ _
Exciter Part Number . . .FF01-531/40-25 Emitter Part Number . . .FF01-593/40-25 Dichroic Part Number . .FF562-Di02-25x36 Full Set Prefix . . . . . . . . .Cy3-4040B-_ _ _
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This filter set is ideal for use with TRITC and a number of Rhodamine dyes, as well as other orange fluorophores.This improved set offers high brightness, and yet with its narrow exciter filter, it also provides a high signal-to-noise ratio and minimizes the bleedthrough in co-labeling applications typical with shorter-wavelength yellow and green fluorescent markers such as YFP, GFP, and FITC.
700
Exciter Part Number . . .FF01-628/40-25 Emitter Part Number . . .FF01-692/40-25 Dichroic Part Number . .FF660-Di01-25x36 Full Set Prefix . . . . . . . . .Cy5-4040A-_ _ _
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This filter set is ideal for use with Texas Red® and other red fluorophores. It also works well for broader orange-to-red fluorophores like MitoTracker Red. It is optimized to provide the highest possible brightness, while maintaining a high signal-to-noise ratio.
This filter set is ideal for use with Cy5™ and other deep red fluorophores. It is optimized to provide the highest brightness, while maintaining a high signal-to-noise ratio.
See page 6 for a list of primary fluorophores. Go to www.semrock.com for ASCII data and a complete fluorophore listing.
12
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Cy5-4040A
Exciter Part Number . . .FF01-562/40-25 Emitter Part Number . . .FF01-624/40-25 Dichroic Part Number . .FF593-Di02-25x36 Full Set Prefix . . . . . . . . .TXRED-4040B-_ _ _
Transmission (%)
600
This filter set is ideal for use with Cy3™ and a variety of other orange fluorophores (including DsRed fluorescent protein). It is optimized to provide the highest brightness, while maintaining a very high signal-tonoise ratio. When co-labeling with YFP, GFP, or FITC, we recommend using our TRITC-A set.
TXRED-4040B
0 500
550
Wavelength (nm)
Wavelength (nm)
80
Dichroic
60
10 500
Emitter
70
20
0 450
Exciter
80
Dichroic
Transmission (%)
Transmission (%)
80
Call us: +49 (0)8153 405-0
Fluorescence Filters BrightLine® Fluorescence Filters BrightLine ZERO™ Fluorescence Filters – for Exact Image Registration
X Ideal for demanding applications like: Co-localization fluorescence measurements Fluorescence In Situ Hibridization (FISH)
BrightLine®
X Allows you to create perfect multi-color composite images
Fluorescence filters 100
Semrock solves your image registration problem – just choose BrightLine ZERO fluorescence filter sets. All of the standard BrightLine single-band filter sets – from our DAPI sets through our Cy5 filter set – are optionally available with certified “zero-pixel-shift” performance (measured relative to other BrightLine ZERO sets).
Comparative Genomic Hybridization (CGH) Property
Set-to-set Image Shift
[1]
Value
< ± 1 pixel
Comment
Worst case image shift when interchanging BrightLine ZERO filter sets, as measured relative to the mean image position for a large sample of filter sets. Analysis assumes collimated light in a standard microscope with a 200 mm focal length tube lens and 6.7 micron pixel size. [1]
Tested in popular microscope cubes.
BrightLine ZERO fluorescence filter sets are very affordable – for a nominal amount more than the price of a BrightLine standard single-band set you can ensure exact image registration when making multi-color composite images. Not sure if you will need this? Keep in mind that BrightLine filters never burn out, and the ZERO option requires no calibration or special alignment – so why not future-proof your system? Our patented design is so manufacturable that BrightLine ZERO filters are in stock for quick delivery. Join your many colleagues and demand the “ZERO option” for certified image registration.
T E C H N I C A L N OT E What is Pixel Shift?
How do we do it?
Pixel shift results when a filter in an imaging path (the emitter and/or dichroic beamsplitter in a fluorescence microscope) with a non-zero wedge angle deviates the light rays so as to cause a shift of the image detected on a high-resolution CCD camera. When two or more images of the same object acquired using different filter sets are overlaid (in order to simultaneously view fluorescence from multiple fluorophores), any significant non-zero filter wedge angle means that the images will not be registered to identical pixels on the CCD camera. Hence, images produced by different fluorophores will not be accurately correlated or combined. On the other hand, BrightLine ZERO filter sets are manufactured, tested and certified to very tight tolerances so as to ensure accurate image registration every time.
Poor image registration, or pixel shift, results from the almost inevitable non-zero filter wedge angle. But low pixel shift is critical to obtain the best imaging performance when exchanging filters during any measurements that involve multiple exposures.
Composite images produced from conventional filter sets (above left), which typically have significant pixel shift, are distorted, whereas BrightLine ZERO pixel shift filter sets (above right) yield precise multi-color images.
For more information go to www.semrock.com to view our article from the August 2005 issue of BioPhotonics International.
Semrock’s advanced ion-beam sputtering (IBS) coating technology makes it possible for all BrightLine filters to be uniquely constructed from a single piece of glass, with the permanent hard coatings applied directly to the outside.This patented[1] lower-loss and high-reliability construction inherently offers superior imaging performance. BrightLine ZERO filter substrates are further manufactured and tested to the most exacting tolerances for certified “zero-pixel-shift” performance.
BrightLine ZERO™ Hard Coating Glass Hard Coating
Conventional Approach Uncoated Adhesive Soft coating Adhesive Soft coating
With older soft-coated fluorescence Uncoated filters, one is forced to use multiple substrates that are typically bonded together with adhesive, generally resulting in significant wedge angle and therefore pixel shift.To improve the imaging registration, extra processing steps, alignment steps, and/or compensating optics are required, resulting in added cost. By contrast, BrightLine ZERO filters are inherently manufacturable and thus very affordable. [1]
U.S. Patent No. 6,809,859 and pending.
www.laser2000.de
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Fluorescence Filters BrightLine® Fluorescence Filters T E C H N I C A L N OT E Fluorescence Resonance Energy Transfer (FRET)
100 90 CFP Exciter CFP Dichroic CFP Emitter
80
Fluorescence Resonance Energy Transfer (FRET) is a powerful technique for characterizing distancedependent interactions on a molecular scale. FRET starts with the excitation of a donor fluorophore molecule by incident light within its absorption spectrum. Normally, the fluorophore would decay radiatively with a characteristic emission spectrum; however, if another fluorophore molecule (the acceptor) exists in close proximity to the donor and with its energy state characterized by an absorption spectrum that overlaps the emission spectrum of the donor, then non-radiative energy transfer may occur between donor and acceptor. As an example, cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) support a strong FRET interaction. FRET is suited to measuring changes in distance on the order of the “Förster distance,” which is typically 20 to 90 Å.This length scale is far below the Rayleigh-criterion resolution limit of an optical microscope (typically 2500 Å for visible light at high numerical aperture), thus illustrating the power of FRET for measuring extremely small distance interactions.
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Figure 1: CFP exciter, dichroic, and emitter filters (CFP-2432A set) for quantitative measurement of donor emission. 100
Application conditions for observing FRET are often characterized by extremely low fluorophore concentrations thus requiring detection of very faint emission levels. Semrock BrightLine® fluorescence filters offer the highest possible transmission for maximizing the FRET emission signal, as well as carefully optimized deep blocking out of the transmission passbands, for maximum possible signal-to-background ratios (highest contrast).
Filter Transmission (%)
90 CFP Exciter
80 70 60 50 40 30 20 10
Figure 1 shows the CFP absorption and emission spectra along with the transmission spectra for a filter set optimized for measuring CFP (CFP-2432A). Figure 2 shows the same set but with a different emitter filter (FF01-536/40) and the emission spectrum for YFP. The first set is used to quantify the fluorescence from the donor alone, while the second set is used to quantify the FRET fluorescence from the acceptor.
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Figure 2: CFP exciter and dichroic filters with an emitter filter (FF01-536/40) for quantitative measurement of the YFP (acceptor) emission.
T E C H N I C A L N OT E Using Fura-2 to Track Ca 2+ Ca -free exciter 2+
The fluorophore Fura-2 has an absorption spectrum that varies markedly depending on the concentration of calcium (Ca2+) that is present near the fluorophore molecule. By measuring the ratio of intensities on digital images captured with two different excitation filters, the variation of calcium concentration at various locations can be tracked. Compared to the highest performance competitive filter sets, the BrightLine FURA2-B set provides 4 times the brightness yet with substantially higher contrast, providing exceptional high-speed imaging performance. Furthermore, the BrightLine set achieves superb balance of the saturated-to-free signal intensities, thus allowing the most accurate ratio calculations with minimal adjustment of camera settings.
Low (Ca2+
2+
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14
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350
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Fluorescence Filters BrightLine® Fluorescence Filters Multiband Fluorescence Filter Sets (pages 15-19) 100 90
Transmission (%)
80 70 60 50 40 30
Measured
20
X Nearly perfect blocking for striking contrast – visually and digitally
10 0 300
X ALL hard dielectric coatings, including blue and UV filters, for long-lasting no “burn-out” performance
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Wavelength (nm) Graph above shows typical measured transmission of the FF01-425/527/685-25 filter
All in Stock – Only at Semrock!
DAPI/FITC/Texas Red in “Full Multiband,” “Pinkel” and “Sedat” Filter Sets Typical measured spectra of the DA/FI/TX “Full Multiband” filter set
Typical measured spectra of the DA/FI/TX “Pinkel” filter set
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3-band Exciter
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3-band Emitter
50 40
3-band Dichroic
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Wavelength (nm)
Filters and Part Numbers for the “Full Multiband” configuration: Exciter Part No. . . . .FF01-407/494/576-25 Emitter Part No. . . . .FF01-457/530/628-25 Dichroic Part No. . .FF436/514/604-Di01-25x36 Full Set Prefix . . . . . .DA/FI/TX-B-_ _ _
Transmission (%)
Transmission (%)
80
70
Exciters 3-band Emitter 3-band Dichroic
60 50 40 30
Filters and Part Numbers for the “Sedat” configuration: Exciter 1 Part No. . .FF01-387/11-25 Exciter 2 Part No. . .FF01-494/20-25 Exciter 3 Part No. . .FF01-575/25-25 Emitter 1 Part No. . .FF02-447/60-25 Emitter 2 Part No. . .FF01-531/22-25 Emitter 3 Part No. . .FF01-624/40-25 Dichroic Part No. . .FF436/514/604-Di01-25x36 Full Set Prefix . . . . .DA/FI/TX-3X3M-A-___
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Wavelength (nm)
Filters and Part Numbers for the “Pinkel” configuration: Exciter 1 Part No. . .FF01-387/11-25 Exciter 2 Part No. . .FF01-494/20-25 Exciter 3 Part No. . .FF01-575/25-25 Emitter Part No. . . .FF01-457/530/628-25 Dichroic Part No. . .FF436/514/604-Di01-25x36 Full Set Prefix . . . . .DA/FI/TX-3X-A-___
This full line of filter sets is optimized for imaging the most popular blue, green, and red fluorophores. The “Full Multiband” set allows simultaneous direct viewing or imaging with a color camera. The “Pinkel” set, with single-band exciters, and the “Sedat” set, with single-band exciters and emitters, are ideal for three-color high-speed imaging using a low-noise monochrome camera and a filter wheel or wheels.
Semrock’s award-winning multiband filter sets are uniquely optimized to provide brilliant colors and a very black background. In this example, the relative signal, noise, and signal-to-noise ratio achieved by a BrightLine DA/FI/TX-B filter set was compared side-by-side with the performance of the premium Full Multiband set of a leading competitor. The BrightLine filters are both 50% brighter and provide a stunning 2.4 times higher contrast.
SNR Signal Noise 1.5
0.6
Go to www.semrock.com for ASCII data and a complete fluorophore listing.
www.laser2000.de
2.4
15
BrightLine®
X The highest transmission and steepest edges for dazzling brightness – visually and digitally
Fluorescence filters 100
Only Semrock manufactures multiband fluorescence filters with passband, edge steepness, and blocking performance that rival the best single-band filters, and all with the superior, no “burn-out” durability of hard coatings. In fact, every filter in every BrightLine filter set, including these multiband sets, is made with the same, durable hard-coating technology (see page 3). So you will always see…
Fluorescence Filters BrightLine® Fluorescence Filters Multiband Sets for Fluorescent Proteins CFP/YFP “Full Multiband,” “Pinkel” and “Sedat” Filter Sets Typical measured spectra of the CFP/YFP “Full Multiband” filter set
NEW!
Typical measured spectra of the CFP/YFP “Pinkel” filter set 100 90
80 2-band Exciter
70 60
2-band Emitter
50
2-band Dichroic
40 30
Transmission (%)
Transmission (%)
100 90
80 70
Exciters
60
2-band Emitter
50
2-band Dichroic
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20
10 0 350 400 450 500 550 600 650 700 750
10 0 350 400 450 500 550 600 650 700 750
Wavelength (nm)
Filters and Part Numbers for the “Full Multiband” configuration: Exciter Part Number . . . . .FF01-416/501-25 Emitter Part Number . . . .FF01-464/547-25 Dichroic Part Number . . .FF440/520-Di01-25x36 Full Set Prefix . . . . . . . . . . .CFP/YFP-A-___
Wavelength (nm)
Filters and Part Numbers for the “Pinkel” configuration: Exciter 1 Part Number . . .FF01-427/10-25 Exciter 2 Part Number . . .FF01-504/12-25 Emitter Part Number . . . .FF01-464/547-25 Dichroic Part Number . . .FF440/520-Di01-25x36 Full Set Prefix . . . . . . . . . . .CFP/YFP-2X-A-___
Filters and Part Numbers for the “Sedat” configuration: Exciter 1 Part Number . .FF01-427/10-25 Exciter 2 Part Number . .FF01-504/12-25 Emitter 1 Part Number . .FF01-472/30-25 Emitter 2 Part Number . .FF01-542/27-25 Dichroic Part Number . .FF440/520-Di01-25x36 Full Set Prefix . . . . . . . . . .CFP/YFP-2X2M-A-___ This full line of filter sets is optimized for imaging samples dual-labeled with cyan and yellow fluorescent proteins (CFP and YFP). The “Full Multiband” set allows simultaneous direct viewing or imaging with a color camera. The “Pinkel” set, with single-band exciters, and the “Sedat” set, with singleband exciters and emitters, are ideal for two-color high-speed imaging using a lownoise monochrome camera and a filter wheel or wheels.
CFP/YFP/HcRed “Pinkel” and “Sedat” Filter Sets Typical measured spectra of the CFP/YFP/HcRed “Pinkel” filter set 100 90
Transmission (%)
80 70
Exciters
60 50
3-band Emitter
40
3-band Dichroic
30 20
10 0 350 400 450 500 550 600 650 700 750
Wavelength (nm)
Filters and Part Numbers for the “Pinkel” configuration: Exciter 1 Part Number . . . . .FF01-427/10-25 Exciter 2 Part Number . . . . .FF01-504/12-25 Exciter 3 Part Number . . . . .FF01-589/15-25 Emitter Part Number . . . . . .FF01-464/542/639-25 Dichroic Part Number . . . . .FF444/521/608-Di01-25x36 Full Set Prefix . . . . . . . . . . . .CFP/YFP/HcRed-3X-A-___ Filters and Part Numbers for the “Sedat” configuration: Exciter 1 Part Number . . . . .FF01-427/10-25 Exciter 2 Part Number . . . . .FF01-504/12-25 Exciter 3 Part Number . . . . .FF01-589/15-25 Emitter 1 Part Number . . . . .FF01-472/30-25 Emitter 2 Part Number . . . . .FF01-542/27-25 Emitter 3 Part Number . . . . .FF01-632/22-25 Dichroic Part Number . . . . .FF444/521/608-Di01-25x36 Full Set Prefix . . . . . . . . . . . .CFP/YFP/HcRed-3X3M-A-___
This composite image was created from separate monochrome images captured using a Pinkel filter set. The sample is a Hela cell expressing SECFP, Venus and mRFP targeted to the endoplasmic reticulum, the mitochondria and the nucleus, respectively. The wide-field image was taken on a Nikon TE2000E inverted microscope with a 60X, PlanApo, 1.4-NA, oil-immersion objective, and a cooled monochrome CCD camera (Orca-ER, Hamamatsu Photonics) using a BrightLine CFP/YFP/HcRed-3X-A filter set.
These filter sets are ideal for imaging samples triple-labeled with cyan and yellow fluorescent proteins (CFP and YFP) and HcRed with high brightness, extremely low crosstalk, and superb signal-tonoise ratio. They are also excellent for samples triple-labeled with CFP, YFP, and an orange or red dye, like Texas Red. The “Pinkel” configuration has three single-band exciters, a triple-band beamsplitter and a triple-band emitter. The “Sedat” configuration has single-band exciters and emitters with a tripleband beamsplitter. The single-band filters are meant to go in a filter wheel or slider.
Go to www.semrock.com for ASCII data and a complete fluorophore listing.
Courtesy of Takeharu Nagai and Kenta Saito, Laboratory for Nanosystems Physiology, REIS, Hokkaido University.
All BrightLine single-band bandpass filters in “Pinkel” and “Sedat” sets, including for Leica microscopes, come with standard 25 mm (32 mm optional) exciters and 25 mm emitters, and are packaged separately for convenient mounting in standard filter wheels. For part numbers for Leica microscopes, see www.semrock.com.
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Call us: +49 (0)8153 405-0
Fluorescence Filters BrightLine® Fluorescence Filters Multiband Sets for Fluorescent Proteins Typical measured spectra of the GFP/DsRed “Pinkel” filter set
Typical measured spectra of the GFP/DsRed “Sedat” filter set
100 90
100 90 80
70 50 40 30 20
Transmission (%)
Transmission (%)
80 60
Improved
Exciters 2-band Emitter 2-band Dichroic
10 0 350 400 450 500 550 600 650 700 750
70 60 50
Exciters
40
Emitters
30
BrightLine®
Fluorescence filters 100
GFP/DsRed “Pinkel” and “Sedat” Filter Sets
2-band Dichroic
20 10 0 350 400 450 500 550 600 650 700 750
Wavelength (nm)
Wavelength (nm)
Exciter 1 Part Number . . . . .FF01-470/22-25 Exciter 2 Part Number . . . . .FF01-556/20-25 Emitter Part Number . . . . . .FF01-512/630-25 Dichroic Part Number . . . . .FF493/574-Di01-25x36 Full Set Prefix . . . . . . . . . . . .GFP/DsRed-2X-A-___
Exciter 1 Part Number . . . . .FF01-470/22-25 Exciter 2 Part Number . . . . .FF01-556/20-25 Emitter 1 Part Number . . . . .FF01-514/30-25 Emitter 2 Part Number . . . . .FF01-617/73-25 Dichroic Part Number . . . . .FF493/574-Di01-25x36 Full Set Prefix . . . . . . . . . . . .GFP/DsRed-2X2M-B-___
These filter sets are ideal for imaging samples dual-labeled with GFP and DsRed with high brightness, extemely low crosstalk, and superb signal-tonoise ratio. They are also ideal for samples dual-labeled with FITC and TRITC, or GFP and Cy3, among other fluorophore combinations. The “Pinkel” set has single-band exciters with a dual-band beamsplitter and dual-band emitter. The “Sedat” set has single-band exciters and emitters with a dualband beamsplitter. The single-band filters are meant to go in a filter wheel or wheels.
GFP/HcRed “Pinkel” Filter Set
BFP/GFP/HcRed “Pinkel” Filter Set
Typical measured spectra of the GFP/HcRed “Pinkel” filter set
Typical measured spectra of the BFP/GFP/HcRed “Pinkel” filter set 100 90
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Transmission (%)
Transmission (%)
100 90
Exciters 2-band Emitter 2-band Dichroic
80 70
Exciters
60
3-band Emitter
50
3-band Dichroic
40 30
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20
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Wavelength (nm)
Wavelength (nm)
Exciter 1 Part Number . . . . .FF01-474/23-25 Exciter 2 Part Number . . . . .FF01-585/29-25 Emitter Part Number . . . . . .FF01-527/645-25 Dichroic Part Number . . . . .FF495/605-Di01-25x36 Full Set Prefix . . . . . . . . . . . .GFP/HcRed-2X-A-___
Exciter 1 Part Number . . . . .FF01-370/36-25 Exciter 2 Part Number . . . . .FF01-474/23-25 Exciter 3 Part Number . . . . .FF01-585/29-25 Emitter Part Number . . . . . .FF01-425/527/685-25 Dichroic Part Number . . . . .FF395/495/610-Di01-25x36 Full Set Prefix . . . . . . . . . . . .BFP/GFP/HcRed-3X-A-___
This filter set is ideal for imaging samples that are dual-labeled with green fluorescent protein (GFP) and HcRed with excellent brightness and minimal crosstalk. It is also excellent for samples dual-labeled with FITC and Texas Red, for example.The green channel is brighter and the red channel is substantially red-shifted compared to the GFP/DsRed-2X-A filter set.
This filter set is ideal for imaging samples that are triple-labeled with blue, green, and red fluorescent proteins (BFP, GFP, and HcRed in particular). It is also excellent for samples with a DAPI (or Hoechst) stain in the blue channel, and other green or red fluorophores like FITC or Texas Red in the green and red channels, respectively. The red band is very wide to allow maximum brightness in this channel.
All BrightLine single-band bandpass filters in “Pinkel” and “Sedat” sets, including for Leica microscopes, come with standard 25 mm (32 mm optional) exciters and 25 mm emitters, and are packaged separately for convenient mounting in standard filter wheels. For part numbers for Leica microscopes, see www.semrock.com. Go to www.semrock.com for ASCII data and a complete fluorophore listing.
www.semrock.com
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17
Fluorescence Filters BrightLine® Fluorescence Filters Quad-band Filter Set – the Flagship of Multiband Sets! Semrock's state-of-the-art DAPI-FITC-TRITC-Cy5 filter sets are ideal for creating the brightest 4-color composite images with superior contrast. The six-filter “Pinkel” set includes exceptionally efficient quad-band beamsplitter and quad-band emission filters mated with four single-band exciters, all of which utilize Semrock’s no “burn-out” hard-coating technology. With its exceptionally low crosstalk, performance rivals the fidelity achieved by single-band sets. The nine-filter “Sedat” set provides the ultimate in highspeed, low-crosstalk imaging performance, with all no “burn-out” hard-coated filters – including the blue and UV filters.
X Revolutionary performance X Most versatile multiband sets on the market X All no “burn-out” exciters
Quad-band “Pinkel” and “Sedat” Filter Sets Typical measured spectra for the Quad-band DA/FI/TR/Cy5 “Pinkel” filter set
400
450
500
550
600
650
700
750
Wavelength (nm)
800
100 90 80 70 60 50 40 30 20 10 0 350
Improved Exciters Emitters 4-band Dichroic
Transmission (%)
Exciters 4-band Emitter 4-band Dichroic
Transmission (%)
100 90 80 70 60 50 40 30 20 10 0 350
Typical measured spectra for the Quad-band DA/FI/TR/Cy5 “Sedat” filter set
400
450
500
550
600
650
700
750
800
Wavelength (nm)
Exciter 1 Part Number . . . . .FF01-387/11-25 Exciter 2 Part Number . . . . .FF01-485/20-25 Exciter 3 Part Number . . . . .FF01-560/25-25 Exciter 4 Part Number . . . . .FF01-650/13-25 Emitter Part Number . . . . . .FF01-440/521/607/700-25 Dichroic Part Number . . . . .FF410/504/582/669-Di01-25x36 Full Set Prefix . . . . . . . . . . . .DA/FI/TR/Cy5-4X-A-___
Leading Competitor’s Quad-band “Pinkel” Filter Set
Exciter 1 Part Number . . . . .FF01-387/11-25 Exciter 2 Part Number . . . . .FF01-485/20-25 Exciter 3 Part Number . . . . .FF01-560/25-25 Exciter 4 Part Number . . . . .FF01-650/13-25 Emitter 1 Part Number . . . . .FF01-440/40-25 Emitter 2 Part Number . . . . .FF01-525/30-25 Emitter 3 Part Number . . . . .FF01-607/36-25 Emitter 4 Part Number . . . . .FF01-684/24-25 Dichroic Part Number . . . . .FF410/504/582/669-Di01-25x36 Full Set Prefix . . . . . . . . . . . .DA/FI/TR/Cy5-4X4M-B-___
Semrock BrightLine Quad-band “Pinkel” Filter Set
Independently tested: 4 times brighter... and twice the contrast! Comparisons done under identical imaging conditions using an Olympus BX61WI microscope outfitted with DSU spinning-disk confocal unit and Hamamatsu ORCA-ER monochrome CCD camera. Sample of Rat Kidney Mesangial Cells courtesy of Mike Davidson, Molecular Expressions™, using: Hoechst 33258, Alexa Fluor 488 – Phalloidin, MitoTracker Red CMXRos, and Vimentin (Ms) – Cy5. Semrock DA/FI/TR/Cy5-4X-A filter set.
Go to www.semrock.com for ASCII data and a complete fluorophore listing.
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Fluorescence Filters BrightLine® Fluorescence Filters T E C H N I C A L N OT E
“Full Multiband” Configuration (Multiband exciter, multiband emitter, & multiband dichroic)
“Full Multiband” Image
Multi-color image captured with a color CCD camera
There are three types of multiband filter sets for simultaneous multicolor imaging. The “full-multiband” configuration uses all multiband filters – exciter, emitter, and dichroic beamsplitter – and is ideal for direct visualization, such as when locating areas of interest on a sample. This approach is quick and easy to implement and is compatible with all standard fluorescence microscopes, though it requires a color camera for electronic imaging and cannot eliminate fluorophore bleedthrough. The “Pinkel” configuration uses singleband exciters in a filter wheel with multiband emitter and dichroic filters, and offers an economical way to achieve very high-speed, high-contrast, simultaneous multi-color imaging. This approach is based on a monochrome CCD camera, which is less expensive and offers better noise performance than color cameras. While bleedthrough is reduced relative to the full-multiband approach, some bleedthrough is still possible since all emission bands are imaged simultaneously. The “Sedat” configuration uses single-band exciters and single-band emitters in synchronized filter wheels, with a multiband dichroic beamsplitter. This approach provides the best image fidelity for high-speed simultaneous multi-color imaging, though it requires a larger investment in system hardware. See www.semrock.com for our 2006 BioPhotonics International article.
“Pinkel” Configuration (Multiband emitter, multiband dichroic, & single-band exciters)
BrightLine®
The ability to label multiple, distinct objects of interest in a single sample greatly enhances the power of fluorescence imaging. For a long time the only way to achieve high-quality images of such samples was to take multiple photographs while switching whole single-band filter cubes between photographs, and then later combine these photographs electronically. Limitations to this approach included “pixel shift” among the multiple, single-color images and the speed with which a complete multi-color image could be captured. Semrock solved the problem of “pixel shift” with its BrightLine ZERO™ technology (see page 13 for a complete explanation), and the single-band filter cube approach remains the best technique for achieving images with the highest contrast and lowest bleedthrough possible. But with increasing demand for high-speed imaging, especially for live-cell real-time analysis using fluorescent protein labels, there is a need for an alternative to the single-band filter cube approach without sacrificing too much image fidelity. Now Semrock’s recent advances in multiband optical filter technology have brought simultaneous multi-color imaging to a new level.
Fluorescence filters 100
Multiband Filter Set Terminology
“Sedat” Configuration
(Multiband dichroic, single-band exciters, & single-band emitters)
“Pinkel” and “Sedat” Composite Image
Single-color images are combined electronically to produce one high-fidelity, multi-color image.
T-Cell and Antigen Presenting Cell (APC) conjugates demonstrating an immunologic synapse. Samples courtesy Beth Graf and Dr. Jim Miller at the University of Rochester Medical Center.
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Fluorescence Filters BrightLine® Fluorescence Filters Multiphoton Filters – Our Hard Coatings are Laser Friendly These BrightLine multiphoton ultrahigh-performance fluorescence filters serve a full range of applications with just two sets of filters, thus conveniently accommodating the wide range of fluorescent dyes that are the essential tools of the modern researcher. The transmission bands of the emitters are so wide that they appear clear at normal incidence, and the reflection bands of the dichroics are so wide that they look like mirrors (at 45° incidence).
Near-UV & Visible Filters
Full Visible Filters
Emitter Short Wave Pass . . . .FF01-680/SP-25 Dichroic Long Wave Pass . . . .FF665-Di01-25x36
Emitter Short Wave Pass . . . .FF01-750/SP-25 Dichroic Long Wave Pass . . . .FF735-Di01-25x36
90
80
80
70
70
Transmission (%)
100
90
60 Multiphoton Fluorescence
50
Ti:Sapphire Laser
40 30 20 10 0 300
NEAR UV!
Transmission (%)
100
400
Emitter Dichroic
60 Multiphoton Fluorescence
50
Ti:Sapphire Laser
40 30 Emitter Dichroic
20 10
500
600
700
800
900
1000
0 300
1100
400
500
Wavelength (nm)
600
700
800
900
1000
1100
Wavelength (nm)
Laser Blocking Emission Filter – FF01-680/SP-25
Laser Blocking Emission Filter – FF01-750/SP-25
Transmission Range (Tavg > 90%) . . . . . . . . . . . . . .350-650 nm Laser Blocking Range . . . . . . . . . . . . . . . . . . . . . . .OD > 8: 680-1040 nm OD > 6: 1040-1080 nm
Transmission Range (Tavg > 90%) . . . . . . . . . . . . . .380-720 nm Laser Blocking Range . . . . . . . . . . . . . . . . . . . . . . .OD > 6: 750-1100 nm
Dichroic Beamsplitter – FF665-Di01-25x36
Transmitted Laser Wavelengths (Tavg > 90%) . . .750-1100 nm Reflection Bands (Ravg > 98%) . . . . . . . . . . . . . . . .350-720 nm
Dichroic Beamsplitter – FF735-Di01-25x36
Transmitted Laser Wavelengths (Tavg > 90%) . . .680-1100 nm Reflection Bands (Ravg > 98%) . . . . . . . . . . . . . . . .350-650 nm
These filters provide exceptional OD > 8 blocking for detecting the lowest fluorophore concentrations, with transmission even down to near-UV wavelengths.
These filters provide excellent detection of fluorescence throughout the full visible wavelength range, including red fluorophores like Cy5™.
All in Stock – Only at Semrock!
Common Specifications Property
Emitter
Dichroic
Comment
Passband Transmission Guaranteed Typical
> 90% > 95%
> 90% > 95%
Averaged over passband
N/A Ultra-low
> 98% Ultra-low
Dichroic Reflection Autofluorescence
Averaged over exciter band(s) Fused silica substrate
Blocking
Emitter filters have exceptional blocking over the Ti:Sapphire laser range as needed to achieve superb signal-to-noise ratios even when using an extended-response PMT or a CCD camera or other silicon-based detector; see www.semrock.com for detailed specifications.
Pulse Dispersion Emitter Orientation
Dichroic beamsplitters are suitable for use with 100 femtosecond gaussian laser pulses. The emitter orientation does not affect its performance; therefore there is no arrow on the ring to denote a preferred orientation. "Reflective coating side" should face toward detector and sample; see diagram on page 8
Dichroic Orientation Microscope Compatibility
These filters fit most standard-sized microscope cubes from Nikon, Olympus, and Zeiss and may also be mounted in optical-bench mounts. Contact Semrock for special filter sizes.
All other specifications are the same as standard BrightLine specifications on page 8.
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Fluorescence Filters BrightLine® Fluorescence Filters T E C H N I C A L N OT E
Multiphoton fluorescence microscopy is similar to traditional fluorescence microscopy in that fluorescent molecules that tag targets of interest in a cell or other specimen are excited and subsequently emit fluorescent photons that are collected to form an image. However, in a two-photon microscope, for example, the molecule is not excited with a single photon, as it is in traditional fluorescence, but instead two photons – each with twice the wavelength – are absorbed simultaneously to excite the molecule (Figure 1). As shown in Figure 2, a typical system is comprised of an excitation laser, scanning and imaging optics, a sensitive detector (usually a photomultiplier tube), and optical filters for separating the fluorescence from the laser (dichroic beamsplitter) and blocking the laser light from reaching the detector (emission filter).
Ti:Sapphire Laser Source
Laser Beam Scan Head
The advantages offered by multiphoton imaging systems include: true three-dimensional imaging, or optical sectioning, like confocal microscopy; the ability to image deep inside of live tissue; elimination of out-of-plane fluorescence; and reduction of photobleaching away Detector from the focal plane to increase sample longevity. In addition, with this method it is possible to Sample image fluorescent dyes with very short Stokes shifts and/or very low efficiencies, and even Figure 2: Typical configuration of a inherently fluorescent molecules native to the multiphoton fluorescence microscope. sample or tissue. Disadvantages of multiphoton imaging include the need for a high-peak-power, pulsed laser, such as a mode-locked Ti:Sapphire laser, and, until now, the lack of high-performance optical filters that provide sufficient throughput across the whole emission range of interest and sufficient blocking across the full laser tuning range (Figure 3). Dichroic Beamsplitter Emitter Filter
Now Semrock has brought enhanced performance to multiphoton users by introducing optical filters with ultra-high transmission in the passbands, very steep transitions, and guaranteed deep blocking everywhere it is needed. Given how much investment is typically required for the excitation laser and other complex elements of multiphoton imaging systems, these new filters represent a simple and inexpensive upgrade to substantially boost system performance. 100 90 70 60 Multiphoton Fluorescence
50 40 30 20
NEAR UV!
Transmission (%)
80
Ti:Sapphire Laser
Measured Emitter Measured Dichroic
10 0 300 400 500 600 700 800 900 1000 1100
Wavelength (nm)
Figure 4: BrightLine multiphoton filters provide nearly ideal performance, as shown in these typical measured spectra of the “Near-UV & Visible” emitter FF01-680/SP and dichroic FF665-Di01.
See www.semrock.com for our recent BioPhotonics International article on multiphoton microscopy.
BrightLine®
Fluorescence filters 100
Multiphoton filters
Figure 1: Two-color in-vivo two-photon imaging from the exposed mouse cortex. NADH fluorescence (red) and sulforhodamine-labeled astrocytes (green) taken using BrightLine FF01-680/SP emitter and FF665-Di01 dichroic. Image courtesy of Karl A. Kasischke and Nikhil Mutyal, Dept. of Neurosurgery, University of Rochester Medical Center.
Ti:Sapphire Laser Tuning Range 2-Photon Excitation
2-Photon Fluorescence 3-Photon Excitation
3-Photon Fluorescence
300 400 500 600 700 800 900 1000 1100
Wavelength (nm) Figure 3: Multiphoton microscopes require control of light over a very wide spectrum: from the near-UV all the way through the near-IR.
The new BrightLine emission filters provide crystal-clear transmission from the near-UV to the near-IR (Figure 4). In fact, by eye the filters look as clear as window glass (Figure 5), in contrast to the brownish tint of traditional filters. At the same time, the dichroic beamsplitters are designed to reflect the precious fluorescence signal with exceptionally high efficiency. The emission filters also provide deep blocking across the Ti:Sapphire laser tuning range, which is critical to achieving high signal-to-noise ratio and measurement sensitivity. Sometimes it is desirable to restrict the spectral band of fluorescence emission detected at any given time, especially when multiple fluorophores are used to label different targets in a sample. Narrower bandpass emission filters are ideal for this purpose, and Semrock provides a wide variety of these bandpass filters (see page 24) that may be comFigure 5: BrightLine multiphoton filters are crystal clear! bined with a multiphoton emitter with almost no loss of fluorescence signal.
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Fluorescence Filters BrightLine® Fluorescence Filters BrightLine Clinical™ Fluorescence Filter Sets – Stunning Value A Dramatic Advance in Clinical Microscopy! First, BrightLine fluorescence filters established the standard in optical filters for research microscopy. Now, after years of continuous development, Semrock has made it possible to adapt the advanced hard-coating Ion Beam Sputtering technology used for BrightLine research filters to the more cost-conscious clinical market. The new BrightLine Clinical fluorescence filter sets are carefully cost-reduced, and yet their optical performance exceeds that of even the premium traditional soft-coated fluorescence filters, while retaining all the proven durability of BrightLine research-grade filters. These all-new patent pending BrightLine Clinical hard-coated fluorescence filter sets are poised to set the standard in affordable performance for clinical laboratories! X Proven reliability and durability – for identical measurements year after year X Dazzling brightness and stunning contrast – for faster, surer diagnoses X Fully cost-competitive – plus no “burn out” so you never have to replace them
Take your laboratory to the next level – ask your microscope dealer to equip your microscope with Semrock’s amazing BrightLine Clinical fluorescence filters.
Hard-coated performance at a soft-coated price™
Color photograph of toxoplasma gondii sample tagged with FITC and Evans Blue and taken using a FITC-LP01-Clinical filter set.
Color photograph of fungal yeast cell sample tagged with Calcofluor White and taken using a CFW-LP01-Clinical filter set.
Performance (Scale of 1 to 5)
5
Comments from independent clinical laboratory personnel
Competitive Filters BrightLine Clinical Filters 4
“Very impressed.” 3
“Dark background makes the fluorescence look more intense.”
2
“Spectacular!” 1
Brightness
Background Appearance
Contrast
Comparison of the performance of FITC-LP01-Clinical filter set to competitive filter sets in widespread use today. Tests were performed at three different hospital clinical laboratories with at least two experts from each laboratory. Testers were asked to rank the performance on a scale of 1 to 5, with 5 representing the best possible performance.
22
“I like this better than the usual milky-white background.”
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Fluorescence Filters BrightLine® Fluorescence Filters CFW-BP01-Clinical
Long Pass Calcofluor White Filter Set
Bandpass Calcofluor White Filter Set
100
100
90
90
Dichroic
Dichroic
80
70
Exciter
60
Transmission (%)
Transmission (%)
80 Emitter
50 40 30
70
40 30 20
10
10 350
400
450
0 300
500
Emitter
50
20
0 300
Exciter
60
BrightLine®
CFW-LP01-Clinical
Fluorescence filters 100
BrightLine Clinical™ Fluorescence Filter Sets (continued)
350
Wavelength (nm)
400
450
500
Wavelength (nm)
These filter sets are optimized for the popular Calcofluor White fluorochrome used in a wide variety of mycological and fungal staining tests and kits. Samples will exhibit amazingly low background and high contrast for easy and accurate identification in test after test. The long-pass (LP01) set offers higher brightness and a dim “blue-white” background to provide clear color contrast (in addition to light-level contrast). The bandpass (BP01) set offers the lowest background and highest light-level contrast in exchange for some brightness.
FITC-LP01-Clinical
FITC-BP01-Clinical
Long Pass FITC Filter Set
Bandpass FITC Filter Set
100
100
90
60
Exciter
Emitter
50 40 30
70 60
30
10
10 500
550
600
0 400
650
Emitter
40
20
450
Exciter
50
20
0 400
Dichroic
80
Transmission (%)
Transmission (%)
70
90
Dichroic
80
450
Wavelength (nm)
500
550
600
650
Wavelength (nm)
These filter sets are optimized for all popular tests based on blue excitation and green fluorescence. These include both direct (DFA) and indirect (IFA) immunofluorescent tests utilizing the FITC fluorochrome. Samples will exhibit amazing brightness and superior contrast for easy and accurate identification in test after test. The long-pass (LP01) set offers higher brightness and the ability to observe longer-wavelength (e.g. yellow, orange, and red) fluorescence from counter-stains such as Rhodamine and Evans Blue. The bandpass (BP01) set is ideal for seeing only green fluorescence and offers higher contrast in exchange for some brightness.
All in Stock – Only at Semrock! Part Number Prefix
Primary Fluorophores
Emitter Type
Emitter Center or Edge Wavelength
Emitter Bandwidth
Exciter Center Wavelength
Exciter Bandwidth
CFW-LP01-Clinical-
Calcofluor White, DAPI
Long Pass
416 nm
N/A
387 nm
11 nm
CFW-BP01-Clinical-
Calcofluor White, DAPI
Bandpass
442 nm
46 nm
387 nm
11 nm
FITC-LP01-Clinical-
FITC, Acridine Orange
Long Pass
515 nm
N/A
475 nm
28 nm
FITC-BP01-Clinical-
FITC, Acridine Orange
Bandpass
529 nm
28 nm
475 nm
28 nm
For full sets mounted in cubes, or filter holders, simply append the cube part number on page 7. For example, CFW-LP01-Clinical-ZHE is a long-pass Calcofluor White set mounted in a Zeiss ZHE cube.
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23
Fluorescence Filters BrightLine® Fluorescence Filters Single-band Bandpass Filters Semrock stocks a wide selection of individual fluorscence bandpass filters that have been optimized for use in a variety of fluorescence instruments. For complete data describing these filters and the most up-to-date listing of available filters please visit www.semrock.com. These single-band bandpass filters all utilize Semrock’s patented single-substrate construction.The substrate is high-optical-quality, low-autofluorescence glass. Unless otherwise noted, all filters are housed in a standard 25 mm round black-anodized aluminum ring with thickness as indicated, and a clear aperture of at least 21 mm. Parts denoted with a “-D” are unmounted. Parts with a “/LP” in the part number are long-pass filters and parts with a “/SP” are short-pass filters. Emission Color
Filter
Center Wavelength
Average Transmission & Bandwidth [1]
Size (Diameter x Thickness)
NEW!
FF01-280/20-25
280 nm
> 65% over 20 nm
25 mm x 5 mm
NEW!
FF01-300/LP-25
306 nm (edge)
> 85% 308-420 nm
25 mm x 5 mm
NEW!
FF01-310/SP-25
293 nm (edge)
> 70% 270-290 nm
25 mm x 3.5 mm
FF01-340/26-25
340 nm
> 75% over 26 nm
25 mm x 5 mm
NEW!
FF01-341/LP-25
347 nm (edge)
> 90% 350-500 nm
25 mm x 3.5 mm
FF01-355/40-25
355 nm
> 80% over 40 nm
25 mm x 3.5 mm
FF01-357/44-25
357 nm
> 75% over 44 nm
25 mm x 5 mm
FF01-370/36-25
370 nm
> 90% over 36 nm
25 mm x 5 mm
FF01-377/50-25
377 nm
> 85% over 50 nm
25 mm x 5 mm
FF01-379/34-25
379 nm
> 90% over 34 nm
25 mm x 5 mm
FF01-386/23-25
386 nm
> 90% over 23 nm
25 mm x 5 mm
FF01-387/11-25
387 nm
> 85% over 11 nm
25 mm x 5 mm
FF01-406/15-25
406 nm
> 85% over 15 nm
25 mm x 3.5 mm
FF01-417/60-25
417 nm
> 90% over 60 nm
25 mm x 5 mm
FF01-427/10-25
427 nm
> 90% over 10 nm
25 mm x 5 mm 25 mm x 5 mm
NEW!
[1]
FF01-438/24-25
438 nm
> 90% over 24 nm
FF01-439/154-25
439 nm
> 90% over 154 nm
25 mm x 5 mm
FF01-440/40-25
440 nm
> 90% over 40 nm
25 mm x 3.5 mm
FF01-445/20-25
445 nm
> 90% over 20 nm
25 mm x 5 mm
FF02 -447/60-25
447 nm
> 90% over 60 nm
25 mm x 3.5 mm
FF01-457/50-25
457 nm
> 90% over 50 nm
25 mm x 5 mm
FF01-460/80-25
460 nm
> 90% over 80 nm
25 mm x 5 mm
FF01-465/30-25
465 nm
> 90% over 30 nm
25 mm x 5 mm
FF01-470/22-25
470 nm
> 90% over 22 nm
25 mm x 5 mm
FF01-472/30-25
472 nm
> 90% over 30 nm
25 mm x 5 mm
FF01-474/23-25
474 nm
> 90% over 23 nm
25 mm x 5 mm
FF01-475/20-25
475 nm
> 90% over 20 nm
25 mm x 3.5 mm
FF01-475/42-25
475 nm
> 90% over 42 nm
25 mm x 5 mm
FF01-475/50-25
475 nm
> 90% over 50 nm
25 mm x 5 mm
FF01-475/64-25
475 nm
> 90% over 64 nm
25 mm x 3.5 mm
FF01-482/21-25
482 nm
> 90% over 21 nm
25 mm x 5 mm
FF01-482/35-25
482 nm
> 90% over 35 nm
25 mm x 5 mm
FF01-483/32-25
483 nm
> 90% over 32 nm
25 mm x 3.5 mm
FF01-485/20-25
485 nm
> 90% over 20 nm
25 mm x 5 mm
FF01-485/70-25
485 nm
> 90% over 70 nm
25 mm x 5 mm
FF01-488/6-25
488 nm
> 90% over 6 nm
25 mm x 3.5 mm
FF01-494/20-25
494 nm
> 90% over 20 nm
25 mm x 5 mm
Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.
(continued)
Blocking varies for different filters. For graphs, ASCII data, and blocking information, go to www.semrock.com.
24
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Fluorescence Filters BrightLine速 Fluorescence Filters Single-band Bandpass Filters (continued) Filter
Center Wavelength
Average Transmission & Bandwidth [1]
Size (Diameter x Thickness)
FF01-494/41-25
494 nm
> 90% over 41 nm
25 mm x 5 mm
FF01-500/15-25
500 nm
> 90% over 15 nm
25 mm x 5 mm
FF01-500/24-25
500 nm
> 90% over 24 nm
25 mm x 5 mm
FF01-504/12-25
504 nm
> 90% over 12 nm
25 mm x 5 mm
FF01-510/10-25
510 nm
> 90% over 10 nm
25 mm x 5 mm
FF01-510/84-25
510 nm
> 90% over 84 nm
25 mm x 3.5 mm
FF01-513/17-25
513 nm
> 90% over 17 nm
25 mm x 3.5 nm
FF01-514/30-25
514 nm
> 90% over 30 nm
25 mm x 3.5 mm
FF01-514/LP-25
527 nm (edge)
> 90% 530-800 nm
25 mm x 5 mm
FF01-520/15-25
520 nm
> 90% over 15 nm
25 mm x 5 mm
FF01-520/35-25
520 nm
> 90% over 35 nm
25 mm x 3.5 mm
FF01-525/20-25
525 nm
> 90% over 20 nm
25 mm x 3.5 mm
FF01-525/30-25
525 nm
> 90 % over 30 nm
25 mm x 3.5 mm
FF01-525/50-25
525 nm
> 90% over 50 nm
25 mm x 5 mm
FF01-528/38-25
528 nm
> 90% over 38 nm
25 mm x 5 mm
FF01-529/24-25
529 nm
> 90% over 24 nm
25 mm x 5 mm
FF01-530/40-25
530 nm
> 90% over 40 nm
25 mm x 3.5 mm
FF01-530/200-25-D
530 nm
> 90% over 200 nm (UV/IR blocking filter)
25 mm x 3 mm (no cell)
FF01-531/22-25
531 nm
> 90% over 22 nm
25 mm x 5 mm
FF01-531/40-25
531 nm
> 90% over 40 nm
25 mm x 5 mm
FF01-534/30-25
534 nm
> 90% over 30 nm
25 mm x 5 mm
FF01-534/42-25
534 nm
> 90% over 42 nm
25 mm x 3.5 mm
FF01-536/40-25
536 nm
>90% over 40 nm
25 mm x 3.5 mm
NEW!
FF01-537/26-25
537 nm
>90% over 26 nm
25 mm x 5 mm
NEW!
FF01-540/15-25
540 nm
> 90% over 15 nm
25 mm x 5 mm
FF01-542/27-25
542 nm
> 90% over 27 nm
25 mm x 3.5 mm
FF01-542/50-25
542 nm
> 90% over 50 nm
25 mm x 5 mm
NEW!
NEW!
NEW!
NEW!
NEW!
[1]
FF01-543/22-25
543 nm
>90% over 22 nm
25 mm x 5 mm
FF01-550/220-25
550 nm
> 90% over 220 nm (UV/IR blocking filter)
25 mm x 5 mm
FF01-554/211-25
554 nm
> 90% over 211 nm (UV/IR blocking filter)
25 mm x 5 mm
FF01-556/20-25
556 nm
> 90% over 20 nm
25 mm x 5 mm
FF01-560/14-25
560 nm
> 90% over 14 nm
25 mm x 5 mm
FF01-560/25-25
560 nm
> 90% over 25 nm
25 mm x 5 mm
FF01-562/40-25
562 nm
> 90% over 40 nm
25 mm x 5 mm
FF01-567/15-25
567 nm
> 90% over 15 nm
25 mm x 3.5 mm
FF01-575/15-25
575 nm
> 90% over 15 nm
25 mm x 5 mm
FF01-575/25-25
575 nm
> 90% over 25 nm
25 mm x 5 mm
FF01-579/34-25
579 nm
> 90% over 34 nm
25 mm x 3.5 mm
FF01-580/14-25
580 nm
> 90% over 14 nm
25 mm x 5 mm
FF01-580/60-25-D
580 nm
> 80% over 60 nm
25 mm x 4 mm (no cell)
FF01-582/15-25
582 nm
> 90% over 15 nm
25 mm x 3.5 mm
FF01-582/75-25
582 nm
> 90% over 75 nm
25 mm x 5 mm
FF01-583/120-25
583 nm
> 90% over 120 nm
25 mm x 3.5 mm
Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.
BrightLine速
Fluorescence filters 100
Emission Color
(continued)
Blocking varies for different filters. For graphs, ASCII data, and blocking information, go to www.semrock.com.
www.laser2000.de
25
Fluorescence Filters BrightLine速 Fluorescence Filters Single-band Bandpass Filters (continued) Emission Color
Filter
Center Wavelength
Average Transmission & Bandwidth [1]
FF01-585/29-25
585 nm
> 90% over 29 nm
25 mm x 5 mm
FF01-585/40-25
585 nm
> 90% over 40 nm
25 mm x 3.5 mm
FF01-587/11-25
587 nm
> 90% over 11 nm
25 mm x 5 mm
FF01-588/21-25
588 nm
> 90% over 21 nm
25 mm x 5 mm
FF01-589/15-25
589 nm
> 90% over 15 nm
25 mm x 5 mm
FF01-590/10-25
590 nm
> 90% over 10 nm
25 mm x 3.5 mm
FF01-590/20-25
590 nm
> 90% over 20 nm
25 mm x 5 mm
FF01-593/40-25
593 nm
> 90% over 40 nm
25 mm x 3.5 mm
NEW!
FF01-600/14-25
600 nm
> 90% over 14 nm
25 mm x 5 mm
NEW!
FF01-607/36-25
607 nm
> 90% over 36 nm
25 mm x 3.5 mm
FF01-609/152-25
609 nm
> 90% over 152 nm
25 mm x 5 mm
FF01-617/73-25
617 nm
> 90% over 73 nm
25 mm x 5 mm
FF01-620/14-25
620 nm
> 90% over 14 nm
25 mm x 5 mm
FF01-624/40-25
624 nm
> 90% over 40 nm
25 mm x 3.5 mm
FF01-625/26-25
625 nm
> 90% over 26 nm
25 mm x 5 mm
FF01-628/40-25
628 nm
> 90% over 40 nm
25 mm x 5 mm
FF01-629/53-25
629 nm
> 90% over 53 nm
25 mm x 5 mm
FF01-630/20-25
630 nm
> 90% over 20 nm
25 mm x 3.5 mm
FF01-632/22-25
632 nm
> 90% over 22 nm
25 mm x 5 mm
FF01-640/14-25
640 nm
> 90% over 14 nm
25 mm x 5 mm
FF01-650/13-25
650 nm
> 90% over 13 nm
25 mm x 5 mm
FF01-660/13-25
660 nm
> 90% over 13 nm
25 mm x 5 mm
FF01-670/30-25
670 nm
> 90% over 30 nm
25 mm x 3.5 mm
FF01-676/29-25
676 nm
> 90% over 29 nm
25 mm x 3.5 mm
> 90% over 13 nm
25 mm x 5 mm
NEW!
NEW!
NEW!
Size (Diameter x Thickness)
FF01-680/13-25
680 nm
FF01-680/SP-25
654 nm (edge)
FF01-684/24-25
684 nm
> 90% over 24 nm
FF01-685/40-25
685 nm
> 90% over 40 nm
25 mm x 5 mm
FF01-692/40-25
692 nm
> 90% over 40 nm
25 mm x 3.5 mm
FF01-697/75-25-D
697 nm
> 90% over 75 nm
25 mm x 4 mm (no cell)
NEW!
FF01-700/13-25
700 nm
> 90% over 13 nm
25 mm x 5 mm
NEW!
FF01-720/13-25
720 nm
> 90% over 13 nm
25 mm x 5 mm
FF01-725/150-25
725 nm
> 90% over 150 nm
25 mm x 5 mm
> 90% over 13 nm
25 mm x 5 mm
NEW!
See Multiphoton Filters, page 20 25 mm x 5 mm
FF01-740/13-25
740 nm
FF01-750/SP-25
727 nm (edge)
NEW!
FF01-760/12-25
760 nm
> 90% over 12 nm
NEW!
FF01-780/12-25
780 nm
> 90% over 12 nm
25 mm x 5 mm
NEW!
FF01-785/62-25
785 nm
> 90% over 62 nm
25 mm x 3.5 mm
FF01-794/160-25
794 nm
> 90% over 160 nm
25 mm x 5 mm
NEW!
FF01-800/12-25
800 nm
> 90% over 12 nm
25 mm x 5 mm
NEW!
FF01-820/12-25
820 nm
> 90% over 12 nm
25 mm x 5 mm
FF01-839/270-25
839 nm
> 90% over 270 nm
25 mm x 5 mm
FF01-840/12-25
840 nm
> 90% over 12 nm
25 mm x 5 mm
NEW!
NEW! [1]
See Multiphoton Filters, page 20 25 mm x 5 mm
Bandwidth is the minimum width over which the average transmission exceeds the specified passband transmission; the nominal full-width-at-half-maximum (FWHM) is approximately the Bandwidth + 1% of the Center Wavelength.
Blocking varies for different filters. For graphs, ASCII data, and blocking information, go to www.semrock.com.
26
Call us: +49 (0)8153 405-0
Fluorescence Filters BrightLine® Fluorescence Filters Multiband Bandpass Filters
0 Measured
Optical Density
Transmission (%)
400
500
600
Wavelength (nm)
700
Measured
1
800
2
See page 42 for a Technical Note on optical density.
3 4 5 6 300
BrightLine®
100 90 80 70 60 50 40 30 20 10 0 300
Fluorescence filters 100
Semrock’s unique ion-beam sputtered filter technology allows us to manufacture the highest-performance multiband filters on the market. Note the extremely high transmission, steep and well-defined edges, and outstanding blocking between the passbands of this triple-band filter (FF01-464/542/639-25) – just one example.
Instrument Noise Limit
400
500
600
700
800
Wavelength (nm)
State-of-the-art Multiband Filters Semrock offers a selection of individual multiband fluorescence bandpass filters that have been optimized for use in a variety of fluorescence instruments. These filters all utilize Semrock’s patented single-substrate construction.The substrate is high-optical-quality, low-autofluorescence glass. All filters are housed in a standard 25 mm round blackanodized aluminum ring with thickness as indicated, and a clear aperture of at least 21 mm. Emission Color
Center Wavelength
Average Transmission & Bandwidth
Size (Diameter x Thickness)
FF01-416/501-25
416 nm 501 nm
> 90% over 25 nm > 90% over 18 nm
25 mm x 5 mm
FF01-448/523-25
448 nm 523 nm
> 90% over 55 nm > 90% over 47 nm
25 mm x 5 mm
FF01-464/547-25
464 nm 547 nm
> 90% over 23 nm > 90% over 31 nm
25 mm x 3.5 mm
FF01-468/624-25
468 nm 624 nm
> 90% over 50 nm > 90% over 35 nm
25 mm x 3.5 mm
FF01-480/593-25
480 nm 593 nm
> 90% over 10 nm > 90% over 120 nm
25 mm x 3.5 mm
FF01-508/585-25
508 nm 585 nm
> 90% over 26 nm > 90% over 72 nm
25 mm x 5 mm
FF01-512/630-25
512 nm 630 nm
> 90% over 23 nm > 90% over 91 nm
25 mm x 3.5 mm
FF01-527/645-25
527 nm 645 nm
> 90% over 42 nm > 90% over 49 nm
25 mm x 3.5 mm
FF01-538/685-25
538 nm 685 nm
> 90% over 50 nm > 90% over 45 nm
25 mm x 3.5 mm
FF01-594/730-25
594 nm 730 nm
> 90% over 42 nm > 90% over 140 nm
25 mm x 5 mm
FF01-407/494/576-25
407 nm 494 nm 576 nm
> 80% over 14 nm > 85% over 20 nm > 85% over 20 nm
25 mm x 5 mm
FF01-425/527/685-25
425 nm 527 nm 685 nm
> 90% over 35 nm > 90% over 42 nm > 90% over 130 nm
25 mm x 3.5 mm
FF01-457/530/628-25
457 nm 530 nm 628 nm
> 80% over 22 nm > 85% over 20 nm > 85% over 28 nm
25 mm x 3.5 mm
FF01-464/542/639-25
464 nm 542 nm 639 nm
> 90% over 23 nm > 90% over 27 nm > 90% over 42 nm
25 mm x 3.5 mm
FF01-480/546/685-25
480 nm 546 nm 685 nm
> 90% over 10 nm > 90% over 22 nm > 90% over 130 nm
25 mm x 3.5 mm
440 nm 521 nm 607 nm 700 nm
> 90% over 40 nm > 90% over 21 nm > 90% over 34 nm > 90% over 45 nm
25 mm x 3.5 mm
Filter
Dual-band Filters NEW! NEW!
NEW!
NEW!
Triple-band Filters
Quadruple-band Filters FF01-440/521/607/700-25
www.laser2000.de
27
Fluorescence Filters BrightLine® Fluorescence Filters Dichroic Beamsplitters – ONLY Hard Coatings Semrock offers a selection of polarization-insensitive dichroic beamsplitters for 45° angle-of-incidence that exhibit steep edges with very high and flat reflection and transmission bands. More complete reflection and transmission mean less stray light for lower background and improved signal-to-noise ratio. These filters are optimized for fluorescence microscopes and instrumentation, and may also be used for a variety of other applications that require beam combining and separation based on wavelength. All Semrock filters are made with our reliable hard-coating technology. Our dichroics utilize high-optical-quality, ultralow-autofluorescence glass substrates. Go to www.semrock.com for ASCII data and spectra graphs.
Single-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°)
Filter
Nominal Edge Wavelength
Reflection Band [1]
Transmission Band [1]
FF310-Di01-25x36
310 nm
255-295 nm
315-600 nm
25.2 mm x 35.6 mm x 1.1 mm
FF409-Di02-25x36
409 nm
344-404 nm
415-570 nm
25.2 mm x 35.6 mm x 1.1 mm
FF458-Di01-25x36
458 nm
426-450 nm
467-600 nm
25.2 mm x 35.6 mm x 1.1 mm
FF495-Di02-25x36
495 nm
442-488 nm
502-730 nm
FF506-Di02-25x36
506 nm
446-500 nm
513-725 nm
FF520-Di01-25x36
520 nm
488-512 nm
528-655 nm
FF555-Di02-25x36
555 nm
493-548 nm
562-745 nm
FF562-Di02-25x36
562 nm
499-555 nm
569-730 nm
25.2 mm x 35.6 mm x 1.1 mm
FF593-Di02-25x36
593 nm
530-585 nm
601-800 nm
25.2 mm x 35.6 mm x 1.1 mm
Emission Color NEW!
[1]
Special Features
Size
25.2 mm x 35.6 mm x 1.1 mm Ideal for separating CFP & YFP emission paths in a 2-detector system
25.2 mm x 35.6 mm x 1.1 mm 25.2 mm x 35.6 mm x 1.1 mm
Ideal for separating GFP & RFP emission paths in a 2-detector system
25.2 mm x 35.6 mm x 1.1 mm
FF655-Di01-25x36
655 nm
470-645 nm
665-726 nm
25.2 mm x 35.6 mm x 1.1 mm
FF660-Di01-25x36
660 nm
594-651 nm
669-726 nm
25.2 mm x 35.6 mm x 1.1 mm
350-660 nm
677-800 nm
FF665-Di01-25x36
665 nm
FF669-Di01-25x36x3.0
669 nm
FF735-Di01-25x36
735 nm
FF740-Di01-25x36
740 nm
See Multiphoton Filters, page 20 25.2 mm x 35.6 mm x 3.0 mm
See Multiphoton Filters, page 20 480-720 nm
750-825 nm
25.2 mm x 35.6 mm x 1.1 mm
Wavelength ranges over which average reflection and transmission are guaranteed to be above 98% and 90%, respectively.
The examples below show high-peformance single-edge dichroic beamsplitters. They are long-wave-pass filters used at 45 degrees and are ideal for epi-fluorescence illumination applications, in which the beamsplitter reflects the excitation light and transmits the fluorescence.The new FF310-Di01 is an ultraviolet (UV) filter that provides very high reflection of light in the 250-300 nm range.
FF310-Di01
100 90
90 80
NEW!
70 60 50 40 30 20
300
350
400
450
Wavelength (nm)
28
500
550
70 60 50 40 30 20
Measured (unpolarized)
10
Transmission (%)
Transmission (%)
80
0 250
FF506-Di02
100
Measured (unpolarized)
10 600
0 400
450
500
550
600
Wavelength (nm)
Call us: +49 (0)8153 405-0
650
700
750
Fluorescence Filters BrightLine® Fluorescence Filters Dichroic Beamsplitters (continued) Nominal Edge Wavelength
Reflection Band [1]
Transmission Band [1]
440 nm 520 nm 462 nm 523 nm 493 nm 574 nm 495 nm 605 nm
415-432 nm 493-511 nm 430-453 nm 508-512 nm 456-480 nm 541-565 nm 454-485 nm 570-598 nm
449-483 nm 530-569 nm 471-489 nm 534-650 nm 500-529 nm 584-679 nm 505-550 nm 620-675 nm
FF497/554-Di01-25x36
497 nm 554 nm
486-490 nm 542-544 nm
420-471 nm 505-525 nm 561-700 nm
Designed for blocking laser wavelengths 488 nm and 543 nm
FF497/661-Di01-25x36
497 nm 661 nm
486-490 nm 646-648 nm
420-471 nm 505-626 nm 668-750 nm
Designed for blocking laser wavelengths 488 nm and 647 nm
FF498/581-Di01-25x36
498 nm 581 nm
486-490 nm 567-569 nm
FF500/646-Di01-25x36
500 nm 646 nm
486-490 nm 632-634 nm
FF502/670-Di01-25x36
502 nm 670 nm 545 nm 650 nm 553 nm 659 nm
350-494 nm 641-660 nm 532.0 nm 632.8 nm 542-544 nm 646-648 nm
FF555/646-Di01-25x36
555 nm 646 nm
542-544 nm 632-634 nm
FF576/661-Di01-25x36
576 nm 661 nm
567-569 nm 646-648 nm
FF579/644-Di01-25x36
579 nm 644 nm
567-569 nm 632-634 nm
420-471 nm 505-549 nm 587-700 nm 420-471 nm 505-613 nm 653-750 nm 505-621 nm 677-800 nm 554-613 nm 658-742 nm 420-525 nm 561-626 nm 668-750 nm 420-525 nm 561-613 nm 653-750 nm 420-549 nm 587-626 nm 668-750 nm 420-549 nm 587-613 nm 653-750 nm
Filter
FF440/520-Di01-25x36 FF462/523-Di01-25x36 FF493/574-Di01-25x36 FF495/605-Di01-25x36
FF545/650-Di01-25x36 FF553/659-Di01-25x36
[1]
Special Features
Size
25.2 mm x 35.6 mm x 1.1 mm 25.2 mm x 35.6 mm x 1.1 mm 25.2 mm x 35.6 mm x 1.1 mm
BrightLine®
Emission Color
Fluorescence filters 100
Dual-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°)
25.2 mm x 35.6 mm x 1.1 mm 25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 1.1 mm
Designed for blocking laser wavelengths 488 nm and 568 nm
25.2 mm x 35.6 mm x 1.1 mm
Designed for reflecting laser wavelengths 488 nm and 633 nm
25.2 mm x 35.6 mm x 1.1 mm Designed for dual-laser excitation (532 and 633 nm) of Cy3 and Cy5 Designed for reflecting laser wavelengths 543 nm and 647 nm Designed for reflecting laser wavelengths 543 nm and 633 nm Designed for reflecting laser wavelengths 568 nm and 647 nm
25.2 mm x 35.6 mm x 1.1 mm 25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 1.1 mm
Wavelength ranges over which average reflection and transmission are guaranteed to be above 95% and 90%, respectively.
Triple-edge Dichroic Beamsplitters (polarization-insensitive; for use at 45°) Emission Color
Filter
FF395/495/610-Di01-25x36
FF436/514/604-Di01-25x36
FF444/521/608-Di01-25x36
FF462/522/607-Di0125x36x3.5 FF494/540/650-Di0125x36x3.5
[1]
Nominal Edge Wavelength
Reflection Band [1]
Transmission Band [1]
395 nm 495 nm 610 nm 436 nm 514 nm 604 nm 444 nm 521 nm 608 nm 462 nm 522 nm 607 nm 494 nm
354-385 nm 465-483 nm 570-596 nm 394-414 nm 484-504 nm 566-586 nm 420-430 nm 496-510 nm 579-596 nm 430-453 nm 507-512 nm 580-595 nm 488 nm (s polarization only) 532 nm 633-642 nm
403-446 nm 502-552 nm 620-750 nm 446-468 nm 520-540 nm 614-642 nm 451-480 nm 530-561 nm 618-664 nm 471-489 nm 532-560 nm 619-750 nm 500-519 nm
540 nm 650 nm
545-610 nm 655-700 nm
Size
25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 1.1 mm
25.2 mm x 35.6 mm x 3.5 mm
25.2 mm x 35.6 mm x 3.5 mm (NBK7 substrate)
Wavelength ranges over which average reflection and transmission are guaranteed to be above 95% and 90%, respectively.
www.laser2000.de
29
Fluorescence Filters
® ® LineBrightLine Fluorescence Filters Fluorescence Filters
oic Beamsplitters (continued) Dichroic Beamsplitters (continued)
ple-edge Dichroic Beamsplitters (polarization-insensitive; use at 45°) Quadruple-edge Dichroic Beamsplitters for (polarization-insensitive; for use at 45°) Filter
Emission Color
FF410/504/582/669Di01-25x36
Nominal Edge Wavelength Filter
Reflection Nominal Edge Band [1] Wavelength
410 nm FF410/504/582/669504 nm Di01-25x36 582 nm 669 nm
381-392 nm 410 nm 475-495 nm 504 nm 547-572 nm 582 nm 643-656 nm 669 nm
Transmission Reflection [1] [1] BandBand
420-460 nm nm 381-392 510-531 nm nm 475-495 589-623 nm nm 547-572 677-722 nm nm 643-656
Transmission Size Band [1]
Size
25.2 420-460 mm x 35.6 nmmm x 1.1 mm 25.2 mm x 35.6 mm x 1.1 mm 510-531 nm 589-623 nm 677-722 nm
gth ranges over which average reflection and which transmission guaranteed be above 95% 90%, respectively. [1] Wavelength ranges over averageare reflection and to transmission are and guaranteed to be above 95% and 90%, respectively.
[1] (polarization-insensitive; ransmittingLaser-transmitting Dichroic Beamsplitters use at 45°) Dichroic Beamsplitters [1] for (polarization-insensitive; for use at 45°)
Transmission Reflection [2] Band [2] Band
Transmission Band [2] Size
499-521 nm Di01-T488/532/638543-623 nm 25x36x5.0 653-755 nm
488 nm 499-521 nm 532 nm 543-623 nm 636-640 nm nm 653-755
Di01-T488/532/568/63825x36x5.0
499-521 nm Di01-T488/532/568/638543-556 nm 25x36x5.0 580-622 nm 652-755 nm
Di01-T488/532/594/63825x36x5.0
499-521 nm Di01-T488/532/594/638543-580 nm 25x36x5.0 608-623 nm 653-755 nm
488 nm 499-521 nm 532 nm 543-556 nm 568 nm 580-622 nm 636-640 nm 652-755 nm 488 nm 499-521 nm 532 nm 543-580 nm 594 nm 608-623 nm 636-640 nm nm 653-755
499-556 nm Di01-T488/568/638580-622 nm 25x36x5.0 652-755 nm
488 nm 499-556 nm 568 nm 580-622 nm 636-640 nm nm 652-755
25.2 mm 488 nmx 35.6 mm x 5.0 mm 25.2 mm x 35.6 mm x 5.0 mm 532 nm 636-640 nm 25.2 mm 488 nmx 35.6 mm x 5.0 mm 25.2 mm x 35.6 mm x 5.0 mm 532 nm 568 nm 636-640 nm 25.2 mm 488 nmx 35.6 mm x 5.0 mm 25.2 mm x 35.6 mm x 5.0 mm 532 nm 594 nm 636-640 nm 25.2 mm 488 nmx 35.6 mm x 5.0 mm 25.2 mm x 35.6 mm x 5.0 mm 568 nm 636-640 nm
Filter
Emission Color
Di01-T488/532/63825x36x5.0
Di01-T488/568/63825x36x5.0
Filter
Reflection Band [2]
Size
amsplitters have [1] highThese and narrow transmission bands on popular laser lines while reflecting fluorescence over the broad reflection bands. beamsplitters have high andcentered narrow transmission bands centered on popular laser lines while reflecting fluorescence over the broad reflection bands. ideal for high-qualityThey imaging with better than 0.75λimaging flatnesswith overbetter the full aperture. are ideal for high-quality than 0.75λ flatness over the full aperture. gth ranges over which the transmission reflection are guaranteed to be above 90%. [2] Wavelength rangesand overaverage which the transmission and average reflection are guaranteed to be above 90%.
Transmission (%)
Transmission (%)
Transmission (%)
Transmission (%)
Di01-T488/568/638 FF410/504/582/669-Di01 FF410/504/582/669-Di01 Di01-T488/568/638 100 100 at the right es at the right The examples 100 100 gh performance show two high 90 90 performance 90 90 eamsplitters. multiband 80 80 beamsplitters. 80 80 488/568/638 The Di01-T488/568/638 70 70 70 70 ree different 60 60 transmits three different 60 60 nd reflects the laser lines 50 and reflects the 50 50 50 uorescence. The generated 40 fluorescence. The 40 40 40 582/669-Di01 FF410/504/582/669-Di01 30 30 30 30 eflecting four is ideal for reflecting four 20 20 20 20 Measured (unpolarized) Measured (unpolarized) citation light andbands of excitation light and Measured (unpolarized) Measured (unpolarized) 10 10 10 10 fluorescence from transmitting0fluorescence from 0 0 t fluorophores four different fluorophores 350700 400 750450 500 550 500 550 600 0 650 700 750 350 600 400 650 450 700 500 750 550 600 650 700 750 500 550 600 650 Wavelength (nm) Wavelength (nm) sly. Wavelength (nm) Wavelength (nm) simultaneously.
Product Specialists Bernhard Dauner +49 (0) 8153-405-17 b.dauner@laser2000.de
Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
30
Call us: +49 (0)8153 405-0
Fluorescence Filters BrightLine速 Fluorescence Filters For the Yokogawa CSU10 & CSU22 Confocal Scanners
Transmitted Laser Wavelengths
Reflection Bands
Semrock Part Number
405 nm, 488 nm, 561-568 nm, 638-647 nm
422-473 nm, 503-545 nm, 586-620 nm, 665-750 nm
Di01-T405/488/568/647-13x15x0.5
405-442 nm, 514 nm, 638-647 nm
458-497 nm, 533-620 nm, 665-750 nm
Di01-T442/514/647-13x15x0.5
405-442 nm, 502-508 nm, 630-641 nm
458-484 nm, 527-607 nm, 664-750 nm
Di01-T442/505/635-13x15x0.5
488 nm, 532 nm
442-473 nm, 503-510 nm, 534-750 nm
Di01-T488/532-13x15x0.5
488 nm, 568 nm
422-473 nm, 503-545 nm, 586-750 nm
Di01-T488/568-13x15x0.5
405-488 nm
508-700 nm
Di01-T488-13x15x0.5
BrightLine速
Dichroic Beamsplitters for the Yokogawa CSU10 and CSU22 confocal scanners These beamsplitters transmit the excitation laser light and reflect the fluorescence from the sample. Because the filters are precisely positioned between the spinning microlens array and pinhole array discs, they have been manufactured to exacting physical and spectral tolerances.The filter dimensions are 13.0 mm x 15.0 mm x 0.5 mm. (Installation in the CSU22 may only be performed by certain Yokogawa-authorized personnel.)
Fluorescence filters 100
Semrock offers fluorescence filters that enable you to achieve superior performance from your real-time confocal microscope system based on the Yokogawa CSU10 or CSU22 scanner. Like all BrightLine filters, they are made exclusively with hard, ion-beam-sputtered coatings to provide unsurpassed brightness and durability.These filters are compatible with all scan head system configurations, regardless of the microscope, camera, and software platforms you have chosen.
Emission Filters for the Yokogawa CSU10 and CSU22 confocal scanners These filters mount outside the CSU head, typically in a filter wheel, and provide the utmost in transmission of the desired fluorescence while blocking the undesired scattered laser light and autofluorescence.The filters are 25.0 mm in diameter and are housed in black-anodized aluminum rings. Blocked Laser Wavelengths
Transmission Bands
Semrock Part Number
405 nm, 488 nm, 561-568 nm
418-472 nm, 583-650 nm
Em01-R405/568-25
405 nm, 442 nm, 561-568 nm, 638-647 nm
458-512 nm, 663-750 nm
Em01-R442/647-25
405 nm, 488 nm
503-552 nm
Em01-R488-25
514 nm
528-650 nm
Em01-R514-25
Laser-blocking Emission Filters for the Yokogawa CSU22 confocal scanner (inside the scan head) These filters go inside the CSU22 head in the motorized emission-filter slider.The purpose is primarily to block undesired laser light, preventing it from exiting the scan head to the camera.The filters are 15.0 mm in diameter and are housed in black anodized aluminum rings. (Installation in the CSU22 may be performed only by certain Yokogawa-authorized personnel.) Blocked Laser Wavelengths
Transmission Bands
Semrock Part Number
405 nm, 442 nm, 514 nm, 638-647 nm
458-497 nm, 529-620 nm, 667-750 nm
Em01-R442/514/647-15
405 nm, 442 nm, 488 nm, 561-568 nm
503-546 nm, 583-700 nm
Em01-R488/568-15
Examples of Popular Fluorophores Used with Common Laser Wavelengths Laser Wavelength(s)
Popular Fluorophores
405 nm
DAPI, Hoechst, Alexa Fluor 405
440, 442 nm
CFP, Alexa Fluor 430
488 nm
GFP, FITC, Alexa Fluor 488
505, 514 nm
YFP
561, 568 nm
DsRed, Rhodamine, Cy3, Texas Red, Alexa Fluor 568
635, 638, 647 nm
Cy5, Alexa Fluor 633 & 647
For graphs of the filter spectra and ASCII data, go to www.semrock.com.
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31
Edge Filters RazorEdge® Raman Filters Semrock RazorEdge thin-film filter technology offers dramatically improved long-wave-pass filters that provide better Stokes-edge steepness and higher transmission than even the leading holographic notch filters, yet are less than half the price! RazorEdge filter technology is so unique that it is patented (U.S. patent No. 7,068,430). X Ultra-steep edges to measure even the smallest Raman shifts X Deep laser-line blocking for maximum laser rejection X High signal transmission to detect the weakest signals X High laser damage threshold and proven reliability X Rejected light is reflected, for convenient alignment and best stray-light control X Ultra-wide, low-ripple passband
RazorEdge Long Wave Pass Edge Filters (25 mm Diameter) Laser Line NEW! NEW!
Transition Width [1]
nm[2]
248.6 < 805 cm 266.0 nm[2] < 372 cm–1 < 737 cm–1 325.0 nm < 305 cm–1 < 603 cm–1 355.0 nm < 279 cm–1 < 552 cm–1 363.8 nm < 272 cm–1 < 539 cm–1 441.6 nm < 224 cm–1 < 444 cm–1 457.9 nm < 216 cm–1 < 428 cm–1 473.0 nm < 209 cm–1 < 415 cm–1 488.0 nm < 203 cm–1 < 402 cm–1 514.5 nm < 192 cm–1 < 381cm–1 532.0 nm < 186 cm–1 < 369 cm–1 [1] [2]
–1
Passband
Part Number
261.0-560.8 nm 272.4-600.0 nm 275.0-600.0 nm 329.2-432.5 nm 332.5-432.5 nm 359.6-800.8 nm 363.2-800.8 nm 368.5-820.6 nm 372.2-820.6 nm 447.3-996.1 nm 451.8-996.1 nm 463.9-668.4 nm 468.4-668.4 nm 479.1-1066.9 nm 483.9-1066.9 nm 494.3-1100.8 nm 499.2-1100.8 nm 521.2-1160.5 nm 526.3-726.3 nm 538.9-1200.0 nm 544.2-1200.0 nm
LP02-248RS-25 LP02-266RU-25 LP02-266RS-25 LP02-325RU-25 LP02-325RS-25 LP02-355RU-25 LP02-355RS-25 LP02-364RU-25 LP02-364RS-25 LP02-442RU-25 LP02-442RS-25 LP02-458RU-25 LP02-458RS-25 LP02-473RU-25 LP02-473RS-25 LP02-488RU-25 LP02-488RS-25 LP02-514RU-25 LP01-514RS-25 LP03-532RU-25 LP03-532RS-25
Laser Line
Transition Width [1]
568.2 nm
< 174 cm < 345 cm–1 632.8 nm < 156 cm–1 < 310 cm–1 647.1 nm < 153 cm–1 < 303 cm–1 664.0 nm < 149 cm–1 < 295 cm–1 780.0 nm < 127 cm–1 < 251 cm–1 785.0 nm < 126 cm–1 < 250 cm–1 808.0 nm < 123 cm–1 < 243 cm–1 830.0 nm < 119 cm–1 < 236 cm–1 980.0 nm < 101 cm–1 < 200 cm–1 1064.0 nm < 93 cm–1 < 184 cm–1
See pages 34 and 38 for more on Transition Width and wavenumbers See additional information on UV RazorEdge filters on page 35
–1
Passband
575.6-1281.7 nm 581.3-1281.7 nm 641.0-1427.4 nm 647.4-1427.4 nm 655.5-1459.6 nm 662.0-1459.6 nm 672.6-1497.7 nm 679.3-1497.7 nm 790.1-1008.0 nm 797.9-1008.0 nm 795.2-1770.7 nm 803.1-1770.7 nm 818.5-1822.6 nm 826.6-1822.6 nm 840.8-1872.2 nm 849.1-1872.2 nm 992.7-2000.0 nm 1002.5-2000.0 nm 1077.8-2000.0 nm 1088.5-2000.0 nm
Part Number
LP02-568RU-25 LP02-568RS-25 LP02-633RU-25 LP02-633RS-25 LP02-647RU-25 LP02-647RS-25 LP02-664RU-25 LP02-664RS-25 LP01-780RU-25 LP01-780RS-25 LP02-785RU-25 LP02-785RS-25 LP02-808RU-25 LP02-808RS-25 LP02-830RU-25 LP02-830RS-25 LP02-980RU-25 LP02-980RS-25 LP02-1064RU-25 LP02-1064RS-25
All in Stock – Only at Semrock!
RazorEdge Long Wave Pass Edge Filters (50 mm Diameter) Laser Line
Long Wave Pass Edge Filters All standard RazorEdge wavelengths available[1] [1]
Part Number
LP0_-___RU-50 LP0_-___RS-50
The “-25” in the part numbers above indicates these filters are 25 mm in diameter. See table on left for 50 mm diameter filters.
Except 248.6 and 266 nm filters – call for availability.
NEW! UV Long Wave Pass RazorEdge filters – see page 35 For a perfectly-matched MaxLine® filter see page 36.
32
www.semrock.com 32
Call us: +49 (0)8153 405-0
Notch Edge Filters Filters RazorEdge® Raman Filters Spectra Examples of RazorEdge Raman Filters Actual measured data from a typical filter is shown! 100
90
90
80
80
70
70
Transmission (%)
Transmission (%)
Transmission spectra of 532 nm RazorEdge filters 100
60 50 40 30 Measured Laser Line
20
60 50 40 30 20
Measured
0 520
525
530
535
540
545
0 450
550
550
650
Wavelength (nm)
Instrument Noise Limit
Optical Density
5 4 3 Measured Laser Line
2 1 0 520
525
530
535
540
850
950
1050
1150
1250
Wavelength (nm)
7 6
750
545
550
Wavelength (nm)
The graph to the left shows a typical measured spectrum of a 532 nm RazorEdge filter plotted on a logarithmic (OD) scale. Note that because RazorEdge filters are so steep (the steepness measured between the OD 6 and 50% transmission points is typically below 0.5% of the edge wavelength!), standard laboratory spectrophotometers are insufficient for measuring the data accurately. Various instrument artifacts appear, including: noise above a certain OD level due to the instrument noise floor; rounded “corners” of the spectrum due to the non-zero bandwidth of monochromators; and edges that appear less steep than they actually are, especially above OD levels of about 3, due to imperfections in the diffraction gratings and other optics that lead to side bands in the monochromator spectrum. Fortunately precise measurements of specific features of the spectrum near the edge (such as OD at the laser wavelength) can be made more painstakingly with special equipment.
Raman filters
10
RazorEdge®
10
RazorEdge Short Wave Pass Edge Filters (25 mm Diameter) These unique filters (U.S. patent No. 7,068,430) are ideal for Anti-Stokes Raman applications. The latest addition to the popular high-performance RazorEdge family of steep edge filters, these new short-wave-pass filters are designed to attenuate a designated laser-line by six orders of magnitude, and yet maintain a typical edge steepness of only 0.5% of the laser wavelength. Both shortand long-wave-pass RazorEdge filters are perfectly matched to Semrock’s popular MaxLine® laser-line cleanup filters.
NEW! NEW!
532.0 nm 632.8 nm 785.0 nm
Transition Width
Passband
< 186 cm–1 < 160 cm–1 < 129 cm–1
350.0-525.2 nm 350.0-624.6 nm 400.0-774.8 nm
Part Number
RazorEdge Short Wave Pass Edge Filters (50 mm Diameter) The “-25” in the part numbers above indicates these filters are 25 mm in diameter. All wavelengths are also available in 50 mm diameter. See the table below for changes to the part numbers. Laser Line
Short Wave Pass Edge Filters All standard RazorEdge wavelengths available
632.8 nm RazorEdge Short Wave Pass Filter
SP01-532RU-25 SP01-633RU-25 SP01-785RU-25
100 90 80
Transmission (%)
Laser Line
70 60 50 40 30 Measured Laser Line
20 10 0 400
450
500
550
600
650
700
750
Wavelength (nm)
Part Number
SP01-___RU-50
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33
Edge Filters RazorEdge® Raman Filters Common Specifications Property
Value
Comment
Edge Steepness (typical)
0.5% of laser wavelength
Blocking at Laser Wavelength Transition Width “U” grade
> 6 OD < 1% of laser wavelength
Measured from OD 6 to 50%; Up to 0.8% for filters below 300 nm OD = – log10 (transmission)
“S” grade Average Passband Transmission Minimum Passband Transmission Passband Wavelength Range Angle of Incidence Cone Half Angle Angle Tuning Range [1] Temperature Dependence Laser Damage Threshold Substrate Material Coating Type Clear Aperture Outer Diameter Overall Thickness Transmitted Wavefront Error Beam Deviation Surface Quality Reliability and Durability
[1]
(2.5 nm or 90 cm–1 for 532 nm filter)
(< 5 nm or 180 cm–1 for 532 nm filter)
Measured from laser wavelength to 50% transmission wavelength
< 2% of laser wavelength
(< 10 nm or 360 cm–1 for 532 nm filter)
> 93% > 90% for 248, 266, and 325 nm filter > 90% > 80% for 248, 266, and 325 nm filter See tables on page 32 0.0 ± 2.0° See technical note on page 40 < 5° Rays uniformly distributed about 0° – 0.3% of laser wavelength Wavelength “blue-shift” attained (– 1.6 nm or + 60 cm–1 for 532 nm filter) by increasing angle from 0° to 8° < 5 ppm / °C < 0.003 nm / °C for 532 nm filter 1 J/cm2 @ 532 nm 10 ns pulse width 0.5 J/cm2 @ 266 nm Tested for 266 and 532 nm filters only Fused silica NBK7 or equivalent for LP01 filters “Hard” ion-beam-sputtered ≥ 22 mm (or ≥ 45 mm) ≥ 21 mm for LP01 filters 25.0 + 0.0 / – 0.1 mm (or 50.0 + 0.0 / – 0.1 mm) Black-anodized aluminum ring 3.5 ± 0.1 mm < λ / 4 RMS at λ = 633 nm (per inch) Peak-to-valley error < 5 x RMS ≤ 30 arc seconds 60-40 scratch-dig 80-50 scratch-dig for LP01 filters Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrate construction for unrivaled filter life. RazorEdge filters are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards. See page 3 for details.
For small angles (in degrees), the wavelength shift near the laser wavelength is Δλ (nm) = – 5.0 × 10 –5 × λL × θ2 and the wavenumber shift is Δ(wavenumbers) (cm–1) = 500 × θ2 / λL, where λL (in nm) is the laser wavelength. See technical note on page 40.
T E C H N I C A L N OT E 100
RazorEdge® and MaxLine® are a Perfect Match
90
Typical Measured Data
80
Transmission (%)
The MaxLine (see page 36) and RazorEdge filters make an ideal filter pair for applications like Raman spectroscopy – they fit together like hand-inglove.The MaxLine filter spectrally “cleans up” the excitation laser light before it reaches the sample under test – allowing only the desired laser line to reach the sample – and then the RazorEdge filter removes the laser line from the light scattered off of the sample, while efficiently transmitting desired light at wavelengths very close to the laser line.
70 60 50 40 30
785 MaxLine 785 RazorEdge
20 10
Typical measured spectral curves of 785 nm filters on a linear transmission plot demonstrate how the incredibly steep edges and high transmission exhibited by both of these filters allow them to be spectrally positioned very close together, while still maintaining complementary transmission and blocking characteristics.
720
740
760
780
800
820
840
880
900
0 1 2
Guaranteed high transmission for RazorEdge and high blocking for MaxLine filter
1%
3 4 5 6
8
785 MaxLine 785 RazorEdge
Laser Line
7 777
781
785
789
793
Wavelength (nm)
34
860
Wavelength (nm)
Optical Density
The optical density plot (see page 42) illustrates the complementary nature of these filters on a logarithmic scale using the theoretical design spectral curves.The MaxLine filter provides very high transmission (> 90%) of light immediately in the vicinity of the laser line, and then rapidly rolls off to achieve very high blocking (> OD 5) at wavelengths within 1% of the laser line.The RazorEdge filter provides extremely high blocking (> OD 6) of the laser line itself, and then rapidly climbs to achieve very high transmission (> 90%) of the desired signal light at wavelengths only 1% away from the laser line.
0 700
Call us: +49 (0)8153 405-0
797
801
805
Edge Filters RazorEdge® Raman Filters NEW! UV Long Wave Pass Edge Filters Semrock’s renowned and patented (U.S. Patent No. 7,068,430) RazorEdge thin-film filters are now available matched to popular ultraviolet (UV) lasers. Due to near-band-edge resonance enhancement effects, and the absence of autofluorescence in the UV, Raman spectroscopy at UV wavelengths can be substantially more sensitive than at visible and near-infrared wavelengths. With the emergence of commercially available UV lasers of sufficient performance, convenience, and price for widespread use, UV Raman spectroscopy is growing rapidly. These unique new UV filters have: X The same ultrasteep edges and deep laser-line blocking of our visible and near-IR RazorEdge filters
Raman filters
X High laser damage threshold and proven reliability
266.0 nm Long Wave Pass Edge Filter Ideal for 4th Harmonic of Nd:YAG lasers
100
100
90
90
80
80
Transmission (%)
Transmission (%)
248.6 nm Long Wave Pass Edge Filter Ideal for new, compact NeCu lasers
70 60 50
Measured Laser Line
40 30
70 60 50 30 20
10
10 225
250
275
300
325
350
Measured Laser Line
40
20 0 200
RazorEdge®
X Superb UV transmission for detecting the weakest Raman signals
0 200
225
250
275
Wavelength (nm)
300
325
350
375
400
Wavelength (nm)
See page 32 for part numbers and page 34 for specifications.
T E C H N I C A L N OT E Ultraviolet (UV) Raman Spectroscopy Raman spectroscopy measurements generally face two limitations: (1) Raman scattering cross sections are tiny, requiring intense lasers and sensitive detection systems just to achieve enough signal; and (2) the signal-to-noise ratio is further limited by fundamental, intrinsic noise sources like sample autofluorescence. Raman measurements are most commonly performed with green, red, or near-infrared (IR) lasers, largely because of the availability of established lasers and detectors at these wavelengths. However, by measuring Raman spectra in the ultraviolet (UV) wavelength range, both of the above limitations can be substantially alleviated.
Raman-active vibrations can increase by many orders of magnitude – this effect is called “resonance-enhanced Raman scattering.”
Visible and near-IR lasers have photon energies below the first electronic transitions of most molecules. However, when the photon energy of the laser lies within the electronic spectrum of a molecule, as is the case for UV lasers and most molecules, the intensity of
Recently an increasing number of compact, affordable, and highpower UV lasers have become widely available, such as quadrupled, diode-pumped Nd:YAG lasers at 266 nm and NeCu hollow-cathode metal-ion lasers at 248.6 nm, making ultra-sensitive UV Raman spectroscopy a now widely accessible technique.
Further, although UV lasers tend to excite strong autofluorescence, it typically occurs only at wavelengths above about 300 nm, independent of the UV laser wavelength. Since even a 4000 cm –1 (very large) Stokes shift leads to Raman emission below 300 nm when excited by a common 266 nm laser, autofluorescence simply does not interfere with the Raman signal making high signal-to-noise ratio measurements possible.
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35
35
Laser-line Filters MaxLine® Laser-line Filters Semrock MaxLine Laser-line Filters have an unprecedented high transmission exceeding 90% at the laser line, while rapidly rolling off to an optical density (OD) > 5 at wavelengths differing by only 1% from the laser wavelength, and OD > 6 at wavelengths differing by only 1.5% from the laser wavelength. This performance is so exclusive that MaxLine filter technology is patented (U.S. patent No. 7,119,960). X Highest laser-line transmission – don’t waste expensive laser light! X Steepest edges – the perfect match to Semrock’s ultra-sharp RazorEdge® filters (see page 32) X Ideal complement to Semrock’s StopLine® deep notch filters (see page 42) for fluorescence and other applications X Hard dielectric coatings for proven reliability and durability
NEW!
Wavelength
Guaranteed Transmission
Typical Bandwidth
OD 5 Blue Range
OD 5 Red Range
12.5 mm Diameter Part Number
25 mm Diameter Part Number
266.0 nm 325.0 nm 355.0 nm 363.8 nm 441.6 nm 457.9 nm 488.0 nm 514.5 nm 532.0 nm 543.5 nm 561.0 nm 568.2 nm 632.8 nm 647.1 nm 780.0 nm 785.0 nm 808.0 nm 830.0 nm 976.0 nm 980.0 nm 1047.1 nm 1064.0 nm
> 55% > 80% > 80% > 85% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90% > 90%
1.9 nm 1.2 nm 1.3 nm 1.4 nm 1.7 nm 1.7 nm 1.9 nm 2.0 nm 2.0 nm 2.1 nm 2.1 nm 2.2 nm 2.4 nm 2.5 nm 3.0 nm 3.0 nm 3.1 nm 3.2 nm 3.7 nm 3.7 nm 4.0 nm 4.0 nm
242.8-263.3 nm 291.0-321.8 nm 314.8-351.5 nm 321.7-360.2 nm 381.0-437.2 nm 393.1-453.3 nm 415.1-483.1 nm 434.1-509.4 nm 446.5-526.7 nm 454.6-538.1 nm 466.7-555.4 nm 471.7-562.5 nm 515.4-626.5 nm 524.8-640.6 nm 609.0-772.2 nm 612.0-777.2 nm 625.9-799.9 nm 639.1-821.7 nm 722.2-966.2 nm 724.4-970.2 nm 963.3-1036.6 nm 978.9-1053.4 nm
268.7-302.2 nm 328.3-380.7 nm 358.6-422.5 nm 367.4-435.0 nm 446.0-551.1 nm 462.5-576.7 nm 492.9-625.3 nm 519.6-669.5 nm 537.3-699.4 nm 548.9-719.5 nm 566.6-750.5 nm 573.9-763.4 nm 639.1-884.7 nm 653.6-912.9 nm 787.8-1201.8 nm 792.9-1213.8 nm 816.1-1139.4 nm 838.3-1183.7 nm 985.8-1325.2 nm 989.8-1332.6 nm 1057.6-1398.6 nm 1074.6-1428.9 nm
LL01-266-12.5 LL01-325-12.5 LL01-355-12.5 LL01-364-12.5 LL01-442-12.5 LL01-458-12.5 LL01-488-12.5 LL01-514-12.5 LL01-532-12.5 LL01-543-12.5 LL01-561-12.5 LL01-568-12.5 LL01-633-12.5 LL01-647-12.5 LL01-780-12.5 LL01-785-12.5 LL01-808-12.5 LL01-830-12.5 LL01-976-12.5 LL01-980-12.5 LL01-1047-12.5 LL01-1064-12.5
LL01-266-25 LL01-325-25 LL01-355-25 LL01-364-25 LL01-442-25 LL01-458-25 LL01-488-25 LL01-514-25 LL01-532-25 LL01-543-25 LL01-561-25 LL01-568-25 LL01-633-25 LL01-647-25 LL01-780-25 LL01-785-25 LL01-808-25 LL01-830-25 LL01-976-25 LL01-980-25 LL01-1047-25 LL01-1064-25
All in Stock – Only at Semrock!
Spectra of MaxLine Filters
Actual measured data from a typical filter is shown! 100
0
90 70
Optical Density
Transmission (%)
80
Design Measured
1
Design Measured
60 50 40 30 20
2 3 4 5
Instrument Noise Limit
10 0 735
745
755
765
775
785
795
Wavelength (nm)
36
805
815
825
835
6 735
745
755
765
775
785
795
805
815
825
Wavelength (nm)
Call us: +49 (0)8153 405-0
835
These graphs demonstrate the outstanding performance of the 785 nm MaxLine laser-line filter, which has transmission guaranteed to exceed 90% at the laser line and OD > 5 blocking less than 1% away from the laser line. Note the excellent agreement with the design curves.
Laser-line Filters MaxLine® Laser-line Filters Spectra of MaxLine Filters
Transmission (%)
80
1064.0
1047.1
980.0
830.0
808.0
785.0
632.8 647.1
532.0 543.5 561.0 568.2
514.5
488.0
441.6 457.9
355.0 363.8
325.0
100
266.0
Actual measured data from typical filters is shown!
Limited wavelength range shown for each filter.
60 40 20 0 250
300
400
500
600
700
800
900
1100
1000
Wavelength (nm)
Illustration of MaxLine filter blocking performance 532 nm filter shown as an example 0
3
OD 5 Red R Rangee OD 6 Blue O Range
4 5
OD 6 Red Range
6
Design
532 nm Laser Line
7 425
450
475
500
525
550
575
600
625
650
675
700
725
MaxLine®
OD 5 B Blue lue Rangee
2
Laser-line filters
Optical Density
1
Wavelength (nm)
Common Specifications Property
Value
Comment
Laser Wavelength λL Transmission at Laser Line
Standard laser wavelengths available > 90%
Bandwidth
See page 36 and www.semrock.com Except λL < 400 nm; see page 36 Will typically be even higher Full Width at Half Maximum (FWHM) Typical 0.7% and Maximum 0.9% for 266 nm filter
0.38% of λL 0.7% of λL OD > 5 from λL ± 1% to 4500 cm –1 OD = – log10 (Transmission) (red shift) and 3600 cm –1 (blue shift); OD > 6 from λL ± 1.5% to 0.92 and 1.10 × λL 0.0 ± 2.0° See technical note on page 40 < 5 ppm / °C < 0.003 nm / °C for 532 nm filter 0.1 J/cm2 @ 532 nm (10 ns pulse width) Tested for 532 nm filter only Low autofluorescence NBK7 or better Fused silica for 266 nm filter “Hard” ion-beam-sputtered ≥ 10 mm (or ≥ 22 mm) For all optical specifications 12.5 + 0.0 / – 0.1 mm Black-anodized aluminum ring (or 25.0 + 0.0 / – 0.1 mm) 3.5 ± 0.1 mm < λ / 4 RMS at = λ 633 nm Peak-to-valley error < 5 × RMS ≤ 11 arc seconds 60-40 scratch-dig Measured within clear aperture Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrate construction for unrivaled filter life. MaxLine filters are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards. See page 3 for details.
Typical Maximum
Blocking[1] Angle of Incidence Temperature Dependence Laser Damage Threshold Substrate Material Coating Type Clear Aperture Outer Diameter Overall Thickness Transmitted Wavefront Error Beam Deviation Surface Quality Reliability and Durability
[1]
The wavelengths associated with these red and blue shifts are given by λ = 1/(1/λL – red shift × 10 –7) and λ = 1/(1/λL + blue shift × 10 –7), respectively, where λ and λL are in nm, and the shifts are in cm –1. Note that the red shifts are 3600 cm–1 for the 808 and 830 nm filters and 2700 cm–1 for the 980 nm filter, and the red and blue shifts are 2400 and 800 cm–1, respectively, for the 1047 and 1064 nm filters. See Technical Note on wavenumbers on page 38.
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37
37
Laser-line Filters MaxLine® Laser-line Filters NEW! High Efficiency Narrowband Laser Clean-up Filters for 266 nm Semrock’s highly acclaimed MaxLine thin-film filters (U.S. Patent No. 7,119,960) are now available for the popular 266 nm quadrupled Nd:YAG laser. These unique new UV filters have: X Unprecedented UV transmission – stop wasting expensive UV laser light! X The same rapid roll-off and deep blocking of our visible and near-IR MaxLine filters X A perfect match to our new 266 nm RazorEdge filters (page 35) X Proven reliability 266.0 nm Narrowband Laser-line Filter Efficiently clean up your quadrupled Nd:YAG Laser 100 90 80 70
Transmission (%)
See page 36 for part numbers and page 37 for specifications.
60 50 40 30 20
Measured
10 0 240
245
250
255
260
265
270
275
280
285
290
Wavelength (nm)
T E C H N I C A L N OT E Measuring Light with Wavelengths and Wavenumbers The “color” of light is generally identified by the distribution of power or intensity as a function of wavelength λ. For example, visible light has a wavelength that ranges from about 400 nm to just over 700 nm. However, sometimes it is convenient to describe light in terms of units called “wavenumbers,” where the wavenumber w is typically measured in units of cm−1 (“inverse centimeters”) and is simply equal to the inverse of the wavelength:
When speaking of a “shift” from a first known wavelength λ1 to a second known wavelength λ2 , the resulting wavelength shift Δλ is given by Δλ = λ2 − λ1 whereas the resulting wavenumber shift Δw is given by Δw =
( λ1
− 1 λ1
2
w(cm−1) = 10 λ(nm) 7
) × 10
7
= − Δλ × 107 λ1λ2
When speaking of a known wavenumber shift Δw from a first known wavelength λ1, the resulting second wavelength λ2 is given by
In applications like Raman spectroscopy, often both wavelength and wavenumber units are used together, leading to potential confusion. For example, laser lines are generally identified by wavelength, but the separation of a particular Raman line from the laser line is generally given by a “wavenumber shift” Δw, since this quantity is fixed by the molecular properties of the material and independent of which laser wavelength is used to excite the line.
λ2 =
1 1/λ1 + Δw × 10−7
Note that when the final wavelength λ2 is longer than the initial wavelength λ1, which corresponds to a “red shift,” in the above equations Δw < 0, consistent with a shift toward smaller values of w. However, when the final wavelength λ2 is shorter than the initial wavelength λ1, which corresponds to a “blue shift,” Δw > 0, consistent with a shift toward larger values of w.
Wavenumbers (cm −1 ) 50,000
33,333
25,000
20,000
16,667
200
300
400
500
600
14,286
12,500
11, 111
10,000
9,091
8,333
700
800
900
1000
1100
1200
Wavelength (nm) UV
38
Near-UV
Visible
Near-IR
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Laser-line Filters T E C H N I C A L N OT E Filter Types for Raman Spectroscopy Applications Raman spectroscopy is widely used today for applications ranging from industrial process control to laboratory research to bio/chemical defense measures. Industries that benefit from this highly specific analysis technique include the chemical, polymer, pharmaceutical, semiconductor, gemology, computer hard disk, and medical Sample fields. In Raman spectroscopy, an intense laser beam is used to create Raman (inelastic) scattered light from a sample under test. The Raman “finger print” is measured by a dispersive or Fourier Transform spectrometer. There are three basic types of Raman Laser Laser Transmitting Blocking instrumentation. Raman microscopes, also called microFilter Filter Raman spectrophotometers, are larger-scale laboratory analytical instruments for making fast, high-accuracy Raman measurements on very small, specific sample areas. Traditional laboratory Raman spectrometers are primarily used for R&D applications, and range from Laser Spectrometer “home-built” to flexible commercial systems that offer a variety of laser sources, means for holding solid and liquid samples, and different filter and spectrometer types. Finally, a rapidly emerging class of Raman instrumentation is the Raman micro-probe analyzer. These complete, compact and often portable systems are ideal for use in the field or in tight manufacturing and process environments. They utilize a remote probe tip that contains optical filters and lenses, connected to the main unit via optical fiber. Optical filters are critical components in Raman spectroscopy systems to prevent all undesired light from reaching the spectrometer and swamping the relatively weak Raman signal. Laser Transmitting Filters inserted between the laser and the sample block all undesired light from the laser (such as broadband spontaneous emission or plasma lines) as well as any Raman scattering or fluorescence generated between the laser and the sample (as in a fiber micro-probe system). Laser Blocking Filters inserted between the sample and the spectrometer block the Rayleigh (elastic) scattered light at the laser wavelength. There are three basic types of filters to choose from: Laser-line Filters, Edge Filters, and Notch Filters. The examples below show how the various filters are used. In these graphs the blue lines represent the filter transmission spectra, the green lines represent the laser spectrum, and the red lines represent the Raman signal. LWP Edge Filter
Notch Filter
Transmission
Transmission
Transmission
Laser-line Filter
Wavelength
Wavelength
Laser-transmitting filter for both Stokes and Anti-Stokes measurements
Laser-blocking steep edge filter for superior Stokes measurements
Wavelength
Versatile laser-blocking notch filter for both Stokes and Anti-Stokes measurements
Laser-Line Filters are ideal for use as Laser Transmitting Filters, and Notch Filters are an obvious choice for Laser Blocking Filters. In systems using these two filter types, both Stokes and Anti-Stokes Raman scattering can be measured simultaneously. However, in many cases Edge Filters provide a superior alternative. For example, a long-wave-pass (LWP) Edge Filter used as a Laser Blocking Filter for measuring Stokes scattering offers better transmission, higher laser-line blocking, and the steepest edge performance to see Raman signals extremely close to the laser line. For more details on choosing between edge filters and notch filters, see the Technical Note “Edge Filters vs. Notch Filters for Raman Instrumentation” on page 44. Semrock stocks high-performance MaxLine® Laser-line Filters, RazorEdge® long-wave-pass and short-wave-pass Edge Filters, and StopLine® Notch Filters as standard catalog products. Non-standard wavelengths and specifications for these filters are routinely manufactured for volume OEM applications.
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39
T E C H N I C A L N OT E Filter Spectra at Non-normal Angles of Incidence Many of the filters in this catalog (with the exception of dichroic beamsplitters and the MaxMirror®) are optimized for use with light at or near normal incidence. However, for some applications it is desirable to understand how the spectral properties change for a non-zero angle of incidence (AOI). There are two main effects exhibited by the filter spectrum as the angle is increased from normal: 1. the features of the spectrum shift to shorter wavelengths; 2. two distinct spectra emerge – one for s-polarized light and one for p-polarized light. RazorEdge® Design Spectra vs. AOI 100 90 80
0˚ 10˚ s
70
Transmission (%)
As an example, the graph at the right shows a series of spectra derived from a typical RazorEdge long-wave-pass (LWP) filter design. Because the designs are so similar for all of the RazorEdge filters in the series, the set of curves in the graph can be applied approximately to any of the filters. Here the wavelength λ is compared to the wavelength λ0 of a particular spectral feature (in this case the edge location) at normal incidence. As can be seen from the spectral curves, as the angle is increased from normal incidence the filter edge shifts toward shorter wavelengths and the edges associated with s- and p-polarized light shift by different amounts. For LWP filters, the edge associated with p-polarized light shifts more than the edge associated with s-polarized light, whereas for short-wave-pass (SWP) filters the opposite is true. Because of this polarization splitting, the spectrum for unpolarized light demonstrates a “shelf” near the 50% transmission point when the splitting significantly exceeds the edge steepness. However, the edge steepness for polarized light remains very high.
10˚ avg 60
10˚ p 30˚ s
50
30˚ avg
40
30˚ p
30
45˚ avg
45˚ s 45˚ p
20 10 0 0.88
0.90
0.92
0.94
0.96
0.98
1.00
1.02
1.04
1.06
1.08
Relative Wavelength (λ/λ0)
The shift of almost any spectral feature can be approximately quantified by a simple model of the wavelength λ of the feature vs. angle of incidence θ, given by the equation:
MaxLine® Design Spectra vs. AOI 100
λ(θ) = λ 0
90
1 – (sinθ/neff)2
80
0˚ 10˚ s
where neff is called the effective index of refraction, and λ0 is the wavelength of the spectral feature of interest at normal incidence. Different shifts that occur for different spectral features and different filters are described by a different effective index. For the RazorEdge example above, the shift of the 90% transmission point on the edge is described by this equation with neff = 2.08 and 1.62 for s- and p-polarized light, respectively.
Transmission (%)
70
10˚ avg 60
10˚ p 30˚ s
50
30˚ avg
40
30˚ p
30
45˚ avg
45˚ s 45˚ p
20 10
Other types of filters don’t necessarily exhibit such a marked difference in the shift of features for s- and p-polarized light. For example, the middle graph shows a series of spectra derived from a typical MaxLine laser-line filter design curve. As the angle is increased from normal incidence, the center wavelength shifts toward shorter wavelengths and the bandwidth broadens slightly for p-polarized light while narrowing for s-polarized light.The center wavelength shifts are described by the above equation with neff = 2.19 and 2.13 for s- and p-polarized light, respectively.The most striking feature is the decrease in transmission for s-polarized light, whereas the transmission remains quite high for p-polarized light.
40
0.90
0.92
0.94
0.96
0.98
1.00
1.02
1.04
1.06
1.08
Relative Wavelength (λ/λ0)
StopLine® Design Spectra vs. AOI 0
1 0˚
2
Optical Density
As another example, the graph at the right shows a series of spectra derived from a typical StopLine notch filter design curve. As the angle is increased from normal incidence, the notch center wavelength shifts to shorter wavelengths, the notch depth decreases, and the notch bandwidth decreases (with a greater decrease for p-polarized light than for s-polarized light).The shift of the notch center wavelength is described by the above equation with neff = 1.76 for both s- and p-polarized light. Note that it is possible to optimize the design of a notch filter to have a very deep notch even at a 45° angle of incidence.
0 0.88
10˚ s 10˚ avg 10˚ p
3
30˚ s 30˚ avg
4
30˚ p 45˚ s 45˚ avg
5
45˚ p 6
7 0.88
0.90
0.92
0.94
0.96
0.98
1.00
1.02
Relative Wavelength (λ/λ0)
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1.04
1.06
1.08
Clean-up Filters MaxDiode™ Laser Diode Clean-up Filters MaxDiode filters have an exceptionally high-transmission, low-ripple passband for consistent high throughput.This remarkable passband is further defined by steep edges for high optical noise discrimination. Keep the desirable laser light while eliminating the noise! The MaxDiode filters are ideal for both volume OEM manufacturers of laser-based fluorescence instrumentation and laboratory researchers who use diode lasers for fluorescence excitation and other types of spectroscopic applications. X Square low-ripple passband for total consistency as the laser ages, over temperature, or when replacing a laser X Highest transmission exceeding 90%, over a carefully tailored range of each diode’s possible laser wavelengths X Extremely steep edges transitioning to very high blocking to filter out the undesired out-of-band noise Laser Diode Wavelength
Center Wavelength
OD 3 Color Range
OD 5 Color Range
12.5 mm Part Number
25 mm Part Number
375 nm 405 nm 440 nm
> 90% over 6 nm > 90% over 10 nm > 90% over 8 nm
375 nm 405 nm 439 nm
212-365 & 385-554 nm 358-389 & 420-466 nm 281-425 & 453-609 nm
337-359 & 393-415 nm 361-384 & 428-457 nm 392-422 & 456-499 nm
LD01-375/6-12.5 LD01-405/10-12.5 LD01-439/8-12.5
LD01-375/6-25 LD01-405/10-25 LD01-439/8-25
470 nm 635 nm
> 90% over 10 nm > 90% over 8 nm
473 nm 640 nm
308-458 & 488-638 nm 400-625 & 655-720 nm
423-455 & 491-537 nm 580-622 & 658-717 nm
LD01-473/10-12.5 LD01-640/8-12.5
LD01-473/10-25 LD01-640/8-25
All in Stock – Only at Semrock!
Spectra of MaxDiode Laser Diode Clean-up Filters Actual measured data from typical filters is shown! 439
473
640
80 60
20 0 300
350
400
450
500
550
600
650
700
Wavelength (nm)
Illustration of MaxDiode filter blocking performance
MaxDiode™
40
Laser-diode filters
Transmission (%)
405
375
100
470 nm filter shown as an example 0 1
Optical Density
NEW!
Transmission & Bandwidth
OD 3 Blue Range g
2 3
OD 3 Red Range g OD 5 Bluee Range g
4 5
OD 5 Red Range g 4700 nm Laser Range n
6 7 300
350
400
450
500
Design 550
600
650
Wavelength (nm)
Common Specifications Property
Value
Transmission over Full Bandwidth Transmission Ripple Blocking Wavelength Ranges Angle of Incidence Performance for Non-collimated Light
> 90% Will typically be even higher < ± 1.5% Measured peak-to-peak across bandwidth Optimized to eliminate spontaneous emission See table above 0.0 ± 5.0° Range for above optical specifications The high-transmission portion of the long-wavelength edge and the low-transmission portion of the short-wavelength edge exhibit a small “blue shift” (shift toward shorter wavelengths). Even for Cone Half Angles as large as 15° at normal incidence, the blue shift is only several nm. Arrow on ring indicates direction of propagation of laser light
Filter Orientation
Comment
All other mechanical specifications are the same as MaxLine® specifications on page 37.
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41
Notch Filters StopLine® Notch Filters StopLine Single-notch Filters Semrock’s StopLine Deep Notch Filters rival the performance of holographic notch filters but in a less expensive, more convenient, and more reliable thin-film filter format.These filters are ideal for applications including Raman spectroscopy, laserbased fluorescence instruments, and biomedical laser systems. Multi-notch filters are also available (see page 45).These filters are so advanced they are patented (U.S. patent No. 7,123,416). X Deep laser-line blocking for maximum laser rejection X Narrowest bandwidth thin-film notch filters X High signal transmission to detect the weakest signals X Negligible temperature dependence for maximum temperature stability X High laser damage threshold and proven reliability X Rejected light is reflected, for convenient alignment and best stray-light control
All in Stock – Only at Semrock!
Spectra of StopLine Single-notch Filters Actual measured data from a typical filter is shown! Wavelength
632.8 nm StopLine Filter
405.0 nm
0
441.6 nm
Optical Density
1 2 NEW!
3 4
568.2 nm 594.1 nm 632.8 nm
5 Design Measured
6 7
500
550
600
650
700
750
800
488.0 nm 514.5 nm 526.5 nm 532.0 nm
785.0 nm 808.0 nm 830.0 nm
850
Wavelength (nm)
Typical 50% Notch Bandwidth
Laser-line Blocking
Part Number
9 nm 12 nm 11 nm 14 nm 14 nm 16 nm 17 nm 17 nm 20 nm 20 nm 22 nm 25 nm 29 nm 39 nm 41 nm 44 nm
OD > 6 OD > 4 OD > 6 OD > 4 OD > 6 OD > 6 OD > 6 OD > 6 OD > 4 OD > 6 OD > 6 OD > 6 OD > 4 OD > 6 OD > 6 OD > 6
NF02-405U-25 NF02-405S-25 NF01-442U-25 NF02-442S-25 NF01-488U-25 NF01-514U-25 NFO1-526U-25 NF01-532U-25 NF02-532S-25 NF01-568U-25 NF01-594U-25 NF01-633U-25 NF02-633S-25 NF01-785U-25 NF01-808U-25 NF01-830U-25
T E C H N I C A L N OT E Working with Optical Density Optical Density – or OD, as it is commonly called – is a convenient tool to describe the transmission of light through a highly blocking optical filter (when the transmission is extremely small). OD is simply defined as the negative of the logarithm (base 10) of the transmission, where the transmission varies between 0 and 1 (OD = – log10(T)). Therefore, the transmission is simply 10 raised to the power of minus the OD (T = 10 – OD). The graph below left demonstrates the power of OD: a variation in transmission of six orders of magnitude (1,000,000 times) is described very simply by OD values ranging between 0 and 6. The table of examples below middle, and the list of “rules” below right, provide some handy tips for quickly converting between OD and transmission. Multiplying and dividing the transmission by two and ten is equivalent to subtracting and adding 0.3 and 1 in OD, respectively. 6
Transmission
Optical Density
5 4 3 2 1 0 1E-6
1E-5
1E-4
1E-3
0.01
Transmission (0-1)
42
0.1
1
OD
1
0
0.5 0.2 0.1
0.3 0.7 1
0.05 0.02 0.01 0.005 0.002 0.001
1.3 1.7 2 2.3 2.7 3
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The “1” Rule T = 1 → OD = 0 The “x 2” Rule T x 2 → OD – 0.3 The “÷ 2” Rule T ÷ 2 → OD + 0.3 The “x 10” Rule T x 10 → OD – 1 The “÷ 10” Rule T ÷ 10 → OD + 1
Notch Filters StopLine® Notch Filters Spectra of StopLine Single-notch Filters 441.6 nm StopLine Filter
1
1
1
2 3 4 5
Design
7 300
350
400
450
500
550
Optical Density
0
6
2 3 4 5
Design
6 7 330
600
380
530
580
1
2 3 4 5
Design
480
530
5
7 360
630
NEW!
580
630
3 4 5
7 400
680
Design
450
500
550
600
650
5
7 400
700
Design
6 625
675
Optical Density
Optical Density
1
2 3 4 5
Design
6 7 450
725
500
550
600
5
650
700
7
750
Design
750
850
950
Optical Density
Optical Density
1
2 3 4 5
Design
6 1050
7 600
700
800
900
600
650
700
750
750
850
1000
2 3 4 5
Design
6 1100
Wavelength (nm)
StopLine®
1
Wavelength (nm)
550
Notch filters
1
650
500
830.0 nm StopLine Filter 0
7 550
Design Measured
Wavelength (nm)
0
4
700
4
808.0 nm StopLine Filter
785.0 nm StopLine Filter
650
3
0
3
600
2
Wavelength (nm)
2
550
6
Wavelength (nm)
6
500
632.8 nm StopLine Filter*
1
5
450
Wavelength (nm)
1
575
Design
6
0
5
660
4
594.1 nm StopLine Filter
4
610
3
0
3
560
2
Wavelength (nm)
2
510
1
2
568.2 nm StopLine Filter
525
460
532.0 nm StopLine Filter
0
475
410
0
Wavelength (nm)
7 425
Design
Wavelength (nm)
6 430
4
6
Optical Density
1
6
Optical Density
480
3
526.5 nm StopLine Filter 0
Optical Density
Optical Density
514.5 nm StopLine Filter 0
7 380
430
2
Wavelength (nm)
Wavelength (nm)
Optical Density
488.0 nm StopLine Filter
0
Optical Density
Optical Density
405.0 nm StopLine Filter 0
7 600
700
800
900
1000
1100
Wavelength (nm)
* Measurement note: Due to the extreme performance of these StopLine notch filters (narrow and very deep notches), it is difficult and time consuming to obtain accurate logarithmic scale measurements, even with the use of a double monochromator. Semrock has carefully measured a typical 633 nm deep notch filter and compared it with the calculated performance (see graph above and on page 42). You can see that the agreement is exceptional – a signature characteristic of Semrock’s highly controlled ion-beam sputtering manufacturing process. Therefore, while we display only theoretical curves for the remaining deep notch filters, we are confident that these are representative curves.
For complete ASCII data and the latest offerings, go to www.semrock.com.
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43
Notch Filters StopLine® Notch Filters Common Specifications for Single-notch Filters Property
Laser Line Blocking:
“U” grade “S” grade “U” grade
Typical 50% Notch Bandwidth
Value
Comment
> 6 OD > 4 OD NBW = 55 × 10 –6 × λL2 + 14 × 10 –3 × λL – 5.9
At the design laser wavelength; OD = – log10 (transmission)
e.g. 17 nm (600 cm–1) for 532.0 nm filter
NBW = 10 × 10 × λL – 29 × 10 × λL + 7.2
“S” grade
–5
2
–3
e.g. 20 nm (700 cm–1) for 532.0 nm filter
Full width at 50% transmission; λL is design laser wavelength (NBW and λL in nm)
Maximum 50% Notch Bandwidth 90% Notch Bandwidth Passband Average Passband Transmission Passband Transmission Ripple Angle of Incidence Angle Tuning Range [2]
< 1.1 × NBW < 1.3 × NBW [1] from 0.75 × λL to λL / 0.75 [1] > 90% < 2.5% 0.0 ± 5.0° – 1% of laser wavelength
Temperature Dependence Laser Damage Threshold Substrate Material Coating Type Clear Aperture Outer Diameter Overall Thickness Transmitted Wavefront Error Surface Quality Reliability and Durability
< 5 ppm / °C 1 J/cm2 @ 532 nm (10 ns pulse width) Ultra-low autofluorescence fused silica “Hard” ion-beam-sputtered ≥ 22 mm For all optical specifications 25.0 + 0.0 / – 0.1 mm Black-anodized aluminum ring 3.5 ± 0.1 mm < λ / 4 RMS at = λ 633 nm Peak-to-valley error < 5 × RMS 60-40 scratch-dig Measured within Clear Aperture Ion-beam-sputtered, hard-coating technology with epoxy-free, single-substrate construction for unrivaled filter life. StopLine filters are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards. See page 3 for details.
Full width at 90% transmission λL is design laser wavelength (nm)
(– 5.3 nm or + 190 cm –1 for 532 nm filter)
Calculated as standard deviation See technical note on page 40 Wavelength “blue-shift” attained by increasing angle from 0° to 14° < 0.003 nm / °C for 532 nm filter Tested for 532 nm filter only
[1]
For NF02-405 filter, 90% bandwidth is < 1.3 × Maximum 50% Bandwidth, and Passband short wavelength is 330 nm.
[2]
For small angles θ (in degrees), the wavelength shift near the laser wavelength is Δλ (nm) = – 5.0 × 10–5 × λL × θ2 and the wavenumber shift is Δ(wavenumbers) (cm–1) = 500 × θ2 / λL, where λL (in nm) is the laser wavelength.
T E C H N I C A L N OT E Edge Filters vs. Notch Filters for Raman Instrumentation RazorEdge Filter Advantages (page 32): • Steepest possible edge for looking at the smallest Stokes shifts • Largest blocking of the laser line for maximum laser rejection
StopLine Notch Filter Advantages (page 42): • Measure Stokes and Anti-Stokes signals simultaneously • Greater angle-tunability and bandwidth for use with variable laser lines
The graph below left illustrates the ability of a long-wave-pass (LWP) filter to get extremely close to the laser line. The graph in the center compares the steepness of an edge filter to that of a notch filter. A steeper edge means a narrower transition width from the laser line to the high-transmission region of the filter. With transition widths guaranteed to be below 1% of the laser wavelength (on Semrock U-grade edge filters), these filters don't need to be angle-tuned! The graph on the right shows the relative tuning ranges that can be achieved for edge filters and notch filters. Semrock edge filters can be tuned up to 0.3% of the laser wavelength.The filters shift toward shorter wavelengths as the angle of incidence is increased from 0° to about 8°. Semrock notch filters can be tuned up to 1.0% of the laser wavelength.These filters also shift toward shorter wavelengths as the angle of incidence is increased from 0° up to about 14°. 0
0
90
1
1
2
2
70
Optical Density
Transmission (%)
80 60 50 40 30 Edge Measured Notch Measured Laser Line
20 10 0
550
600
650
700
Wavelength (nm)
44
750
800
Optical Density
100
3 4 5
3 4 5 6
6
Edge Design Notch Design Laser Line
7
Edge Design Notch Design Laser Line
7
8 610 615 620 625 630 635 640 645 650 655 660
8 610 615 620 625 630 635 640 645 650 655 660
Wavelength (nm)
Wavelength (nm)
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Notch Filters StopLine® Notch Filters StopLine Multi-notch Filters Semrock’s unique multi-notch filters meet or exceed even the most demanding requirements of our OEM customers. These include dual-, triple-, and even quadruple-notch filters for a variety of multi-laser instruments. Applications include: X Laser-based fluorescence instruments X Confocal and multi-photon fluorescence microscopes X Analytical and medical laser systems Semrock’s advanced manufacturing process means that we can make these filters with notch wavelengths that are not integer multiples of each other!
All in Stock – Only at Semrock!
StopLine multi-notch catalog filters include: Laser Colors
Laser Wavelengths
Laser-line Blocking
Part Number
Dimensions
488 & 532 nm
OD > 6
NF01-488/532-25x5.0
25 x 5.0 mm
488 & 543 nm
OD > 6
NF01-488/543-25x5.0
25 x 5.0 mm
486-490 & 631-640 nm
OD > 4
NF01-488/635-25.4x6.0-D
25.4 x 6.0 mm (Note: no housing)
488 & 647 nm
OD > 6
NF01-488/647-25x5.0
25 x 5.0 mm
543 & 647 nm
OD > 6
NF01-543/647-25x5.0
25 x 5.0 mm
568 & 638 nm
OD > 6
NF01-568/638-25x5.0
25 x 5.0 mm
568 & 647 nm
OD > 6
NF01-568/647-25x5.0
25 x 5.0 mm
594 & 638 nm
OD > 6
NF01-594/638-25x5.0
25 x 5.0 mm
Dual-notch Filters
Triple-notch Filters 405, 488, & 568 nm
OD > 6
NF01-405/488/568-25x3.5
25 x 3.5 mm
488, 532, & 631-640 nm
OD > 4
NF01-488/532/635-25x5.0
25 x 5.0 mm
400-410, 488, 532, & 631-640 nm
OD > 4
NF01-405/488/532/635-25x5.0
25 x 5.0 mm
400-410, 488, 561, & 631-640 nm
OD > 4
NF01-405/488/561/635-25x5.0
25 x 5.0 mm
Quadruple-notch Filters
StopLine filters are housed in a 25 mm black-anodized aluminum ring unless indicated otherwise. All filters are on low-autofluorescence NBK7 or ultra-low-autofluorescence fused silica substrates.
Examples of StopLine Multi-notch Filters – Actual measured data from typical filters is shown! 405 & 488 & 568 nm Multi-notch Filter
400-410, 488, 532, & 631-640 nm Multi-notch Filter
1
2
Optical Density
Optical Density
1
3 4 5
7 400
450
500
550
600
650
3 4 5
Design Measured
6
2
700
StopLine®
0
Notch filters
0
6 400
Wavelength (nm)
Design Measured 450
500
550
600
650
700
Wavelength (nm)
For complete graphs, ASCII data, and the latest offerings, go to www.semrock.com.
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45
T E C H N I C A L N OT E Laser Damage Threshold Laser damage to optical filters is strongly dependent on many factors, and thus it is difficult to guarantee the performance of a filter in all possible circumstances. Nevertheless, it is useful to identify a Laser Damage Threshold (LDT) of pulse fluence or intensity below which no damage is likely to occur. Pulsed vs. continuous-wave lasers: Pulsed lasers emit light in a series of pulses of duration τ at a repetition rate R, with peak power Ppeak. Continuous-wave (cw) lasers emit a steady beam of light with a constant power. Pulsed-laser average power Pavg and cw laser constant power typically range from several milli-Watts (mW) to Watts (W) for most lasers. The diagram and table below illustrate and summarize the key parameters that are used to characterize the output of pulsed lasers.
Symbol τ R
P
Definition Pulse duration Repetition rate
Units sec Hz = sec–1
Key Relationships τ=D/R R=D/τ
D P E A I
Duty cycle Power Energy per pulse Area of laser spot Intensity
dimensionless Watts = Joules / sec Joules cm2 Watts / cm2
D=Rxτ Ppeak = E / τ; Pavg = Ppeak x D; Pavg = E x R E = Ppeak x τ; E = Pavg / R A = (π / 4) x diameter2 I = P / A; Ipeak = F / τ; Iavg = Ipeak x D; Iavg = F x R
F
Fluence per pulse
Joules / cm2
F = E / A; F = Ipeak x τ; F = Iavg / R
Ppeak 1/R Pavg time
Note that because fluence and intensity on the surface of the component are the critical parameters, the area of the laser spot is also critical. Even very high-power lasers may be transmitted through, or reflected off of, a durable optical filter if the spot size is sufficiently large to minimize the fluence and/or intensity. The diameter of a laser spot with a Gaussian profile is most commonly measured at the 1/e 2 intensity points as shown in the diagram below.
Long-pulse lasers: LDT is perhaps most accurately specified in terms of pulse I(r) fluence for “long-pulse lasers.” Long-pulse lasers have pulse durations τ in the I0 nanosecond (ns) to microsecond (μs) range, with repetition rates R typically ranging from about 1 to 100 Hz. Because the time between pulses is so large (milliseconds), the irradiated material is able to thermally relax – as a result damage is generally not heat-induced, but rather caused by nearly I 0 /e 2 instantaneous optical field effects. Usually damage results from surface diameter or volume imperfections in the material and the associated irregular r optical field properties near these sites, rather than catastrophic destruction of the fundamental material structure. Most Semrock filters have LDT values on the order of 1 J/cm2, and are thus considered “high-power laser quality” components. An important exception is a High-Q laser-line filter in which the internal field strength is strongly magnified, resulting in an LDT that may be an order of magnitude smaller. As an example, suppose a frequency-doubled Nd:YAG laser at 532 nm emits 10 ns pulses at a 10 Hz repetition rate with 1 W of average power. This laser has a duty cycle of 1 x 10 –7, a pulse energy of 100 mJ, and a peak power of 100 MW. If the beam is focused down to a 100 μm diameter spot on the surface of a component, the pulse fluence is 1.3 kJ/cm2, and thus it will almost surely damage a component with a 1 J/cm 2 LDT. However, if the spot diameter is 5 mm, the pulse fluence is only 0.5 J/cm2, and thus the component should not be damaged. cw lasers: The LDT for cw lasers is more difficult to measure, and therefore is not specified as often as the long-pulse laser LDT. Damage from cw lasers tends to result from thermal (heating) effects. At this time Semrock does not test nor specify cw LDT for its filters. As a very rough rule of thumb, many all-glass components like dielectric thin-film mirrors and filters have a cw LDT (specified as intensity in kW/cm2) that is 10 – 100 times the long-pulse laser LDT (specified as fluence in J/cm2). Quasi-cw lasers: Quasi-cw lasers are pulsed lasers with pulse durations τ in the femtosecond (fs) to picosecond (ps) range, and with repetition rates R typically ranging from about 10 – 100 MHz for high-power lasers. These lasers are typically mode-locked, which means that R is determined by the round-trip time for light within the laser cavity. With such high repetition rates, the time between pulses is so short that thermal relaxation cannot occur. Thus quasi-cw lasers are often treated approximately like cw lasers with respect to LDT, using the average intensity in place of the cw intensity.
46
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MaxMirror® MaxMirror® Ultra-broadband High-Performance Mirror
Winner of the 2003 Photonics Circle of Excellence award!
The MaxMirror is a unique high-performance laser mirror that covers an ultra-broad range of wavelengths – it can replace three or more conventional laser mirrors. In fact, it is so unique that it is patented (U.S. patent No. 6,894,838). The MaxMirror is a winner of the prestigious Photonics Circle of Excellence award, reserved for the most innovative new products of the year. And there is still nothing else like it on the market! X Very highly reflecting over: Near-UV, all Visible, and Near-IR wavelengths All states of polarization All angles from 0 to 50° inclusive simultaneously! X High laser damage threshold and proven reliability X Low-scattering Property
Value
Wavelength Range Standard Reflectivity
350-1100 nm > 99.0% > 98.5% (> 99% typical) > 98.5% (> 99% typical) 45.0 ± 2.5° 0.0 ± 5.0° R > 98.5% R > 98.0% R > 98.0% 0.0-50.0°
Standard Angle of Incidence Wide Angle Reflectivity
Wide Angle of Incidence Range Laser Damage Threshold
Comment
For unpolarized light For “s” polarization For “p” polarization Range over which Standard Reflectivity specifications are met For unpolarized light For “s” polarization For “p” polarization Range over which Wide Angle Reflectivity specifications are met ~ 10 ns pulse width
1 J/cm2 @ 355 and 532 nm 2 J/cm2 @ 1064 nm NBK7 or equivalent “Hard” ion-beam-sputtered > 80% of Outer Diameter 25.0 or 25.4 or 50.8 mm + 0.0 / – 0.25 mm 9.52 ± 0.25 mm See table below Measured at λ = 633 nm 20-10 scratch-dig Measured within Clear Aperture 0.75 mm maximum The MaxMirror will not introduce appreciable pulse broadening for most laser pulses that are > 1 picosecond; however, pulse distortion is likely for significantly shorter laser pulses, including femtosecond pulses. Ion-beam-sputtered, hard-coating technology with unrivaled filter life. MaxMirror ultra-broadband mirrors are rigorously tested and proven to MIL-STD-810F and MIL-C-48497A environmental standards; see page 3 for details.
Substrate Material Coating Type Clear Aperture Outer Diameter Thickness Mirror Side Surface Flatness Mirror Side Surface Quality Mirror Side Bevel Pulse Dispersion Reliability and Durability
Typical MaxMirror spectrum Actual measured data shown.
Reflectivity (%)
80 70 “s” polarization “p” polarization
60 50 40
Surface Flatness
Mirror Side Part Number
25.0 mm 25.4 mm (1.00”) 50.8 mm (2.00”)
< λ / 10 < λ / 10 <λ/4
MM1-311-25.0 MM1-311-25.4 MM1-311-50.8
Measured at 45˚ Also highly reflecting 0 – 50˚
30 20
All in Stock – Only at Semrock!
10 0 300
Diameter
Laser mirror
90
MaxMirror®
100
400
500
600
700
800
900 1000 1100 1200
Wavelength (nm)
www.laser2000.de
47
Partner in the European Photonics Market Your
Over more than 20 years Laser 2000 is concentrating on the three core product areas of Photonics market. Our experts and experienced account managers ensure the best possible attention to your requirements today for the solutions of tomorrow.
Laser, Lightsources & Accessories
Image Processing & Machine Vision
Fiber Optics & Digital Testing
Whether products for industry or for research, Laser 2000 recognizes and understands the customer’s needs. We supply OEM services and provide all solution related components that can be built around or be integrated into the application of your customer.
To improve productivity and quality in industrial environments we support the increasing demand for photonics products. Our engineers assist customers in selecting the appropriate combination of light source, camera and software.
Customers generate profit from a full range of components and systems. Our sales engineers assist in developing solutions. Our seminars are recognized as a source of leading edge information.
Gas lasers Solid state lasers Laser diodes and laser diode modules Measurement and control equipment Optical, opto-mechanical and safety products
48
Light sources Line and array camera systems Test charts and calibration patterns Software
Call us: +49 (0)8153 405-0
ctive and passive components for A Telecom and Datacom Instrumentation for Telecom and Datacom Field test and measurement equipment for Optical Networking
fs-Lasers Femtosecond Lasers s-Pulse series
The new generation of ultrafast lasers from Amplitude Systemes provides diode-pumped femtosecond lasers setting new standards for performance and easy to use. They take full advantage of new, high quality ytterbium doped materials. Ytterbium lasers can be directly diode-pumped and do not require any additional green pump laser. They also offer a number of attractive properties, such as a very high thermal efficiency, as well as the capability to generate femtosecond pulses with a high average power.
The s-Pulse series of amplified femtosecond lasers offers high pulse energies at high repetition rates. The s-Pulse is built on a compact, small footprint, rugged mechanical base for improved stability. It features simple control electronics and no requirement for external water cooling. Low electrical consumption and long lifetime laser diodes ensure operating costs are at a minimum. The short pulse duration and the high output beam quality makes the s-Pulse series ideal for
Laser ablation Marking and engraving Laser assisted ICP-MS Eye surgery Non linear optics
t-Pulse series
DsRed
GFP & Covalaria
YFP
GFP
Microstructures on metal, ceramics and wafers
Femtosecond laser t-Pulse 20
The t-Pulse series provides compact and reliable femtosecond lasers with high average power, exceptional energy per pulse and excellent pulse-to-pulse stability. With its low electrical consumption and its internal cooling system, the tPulse is designed for simple daily operation, a short warm-up time and an excellent day to day repeatability. It is the laser of choice for applications like
Product Specialists Mark Drechsler +49 (0) 8153-405-54 m.drechsler@laser2000.de Dr. Stefan Kremser +49 (0) 8153-405-16 s.kremser@laser2000.de
Multiphoton spectroscopy and microscopy Photoacoustics Terahertz imaging Photopolymerization Non linear optics
Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de t-Pulse
s-Pulse
Specifications
t-Pulse 20
t-Pulse 200
t-Pulse 500
s-Pulse
s-Pulse HR
Wavelength
1030 nm
1030 nm
1030 nm
1030 nm
1030 nm
s-Pulse HP 1030 nm
20 nJ
200 nJ
500 nJ
100 µJ
10 µJ
30 µJ
Repetition Rate
50 MHz
10 MHz
10 MHz
1…10 kHz
100 kHz
100 kHz
Average Power
1W
2W
5W
0,1…1 W
1W
3W
< 200 fs
< 400 fs
< 500 fs
< 400 fs
< 500 fs
< 500 fs
> 100 kW
> 500 kW
> 1 MW
> 250 MW
> 20 MW
> 60 MW
Pulse Energy
Pulse Duration Peak Power Foot print
60 x 20 cm
60 x 40 cm
75 x 50 cm
Pulse Picker
x
x
x
x
x
x
Second Harmonic Generation (SHG)
x
x
x
x
x
x
Third Harmonic Generation (THG)
x
x
x
x
x
x
Pulse Compression
x
x
Tunability
x
www.laser2000.de
49
DPSS Lasers Diode pumped solid state lasers
SLIM laser head
SLIM laser head and OEM driver
Blue, green and yellow SLIM DPSS lasers
The SLIM platform is a new groundbreaking generation of continuous-wave laser modules featuring outstanding optical performances in a miniaturized package. Based on Oxxius‘ proprietary Alignment-free Monolithic Resonator (AMR) technology, it delivers light beams with characteristics particularly well suited to a wide range of instrumentation and scientific applications: single-frequency spectrum, low noise and long-term power stability.
SLM: Low-noise and single longitudinal mode cw operation Ruggedness: The laser cavity cannot be misaligned by thermal or mechanical stress.
Reliability: Highly reliable GaAs laser diodes are used to pump the lasing medium.
Highest compactness: No micro-mechanical positioning or aligning element is required; the size of the optical laser head can be less than a cubic inch.
Because of its unparalleled compactness, it is a unique yet affordable light source for a large variety of applications including laser induced fluorescence microscopy, flow cytometry, Raman spectroscopy, metrology, holography, display and projection.
Efficiency: Because high internal intensities are achievable, frequency doubling can be efficient with pump powers as low as one Watt.
Scalable power levels: Due to the thermal properties of crystals, Watt-scale operation can be achieved without degradation.
All members of the SLIM family have a market-leading size which makes possible the development of reduced-footprint instruments. In addition, because of their low power consumption, they can be embedded in mobile or portable devices.
High spectral purity: With AMR’s millimeter-scale cavity length, single longitudinal mode (SLM) operation can easily be achieved, with a linewidth smaller than 1 MHz and a high signal-to-noise ratio.
Versatility: With AMR, numerous laser wavelengths can
Lasers based on the SLIM platform are available today at 473 nm, 532 nm and 561 nm. They can be provided in beam-shaping and packaging configurations optimized to their target environment.
be achieved and will be introduced in the near future.
SLIM-473 Parameters
Uncollimated
Wavelength Power Stability
50
Collimated
561 nm up to 25 mW +/- 1% over 24 hours
< 0.2%
< 0.2%
< 0.2%
< 1 MHz
< 1 MHz
< 1 MHz
<1.2
<1.2
<1.2
TEM00
TEM00
TEM00
0.2 mm
0.7 mm
< 10 mrad
< 1.5 mrad
100:1, Linear
Circularity Width x Height
532 nm up to 150 mW
Beam Mode
Length
Uncollimated
+/- 1% over 24 hours
M2
Polarization
SLIM-561
Collimated
20 / 40 mW
Linewidth
Beam Divergence
Uncollimated
+/- 1% over 24 hours
Optical Noise (RMS, 10 Hz - 100 MHz)
Beam Diameter (at window, typ.)
SLIM-532
Collimated
473 nm
CW Power
Collimated SLIM laser head and CE driver
0.2 mm
1 mm
0.2 mm
0.7 mm
< 10 mrad
< 2 mrad
< 10 mrad
< 2 mrad
100:1, Linear > 0.85
50 mm
100:1, Linear > 0.85
80 mm
44 mm x 25 mm
50 mm
> 0.85 80 mm
44 mm x 25 mm
Call us: +49 (0)8153 405-0
50 mm
80 mm
44 mm x 25 mm
DPSS Lasers CW DPSS lasers from Ultraviolet to Infrared
A wide variety of continious wave (CW) lasers is available at the following wavelengths: 355 nm, 375 nm, 405 nm, 430 nm, 440 nm, 457 nm, 473 nm, 501 nm, 523 nm, 532 nm, 543 nm, 556 nm, 561 nm, 589 nm, 593 nm, 612 nm, 633 nm, 635 nm, 671 nm, 690 nm and others.
For full information use the Laser 2000 Web Site www.laser2000.de
Pulsed UV Lasers for Molecular Uncaging, Microdissection, MALDI TOF and Bio-Particle Detection The fast growing fields of bio-science are now taking advantage of the latest developments in DPSS laser technology. The Model DPS-3507-50 offers the precise pulse duration, power stability and UV wavelength parameters needed for photolisys of caged compounds. Molecular uncaging through UV photolisys is providing new avenues of understanding into neurological disorders and drug delivery methods.
DPS-3507-50 complete laser system for Molecular Uncaging, Bio-Particle Detection
LCL-GARNET laser for MALDI TOF, Laser microdissection
Wavelength (nm)
Pulse Energy (µJ at 1 kHz)
Beam diameter (mm)
Beam divergence (half angle, mrad)
Polarization
LCL-LCS-DTL-378QT
351
40
<1
<5
>100:1, horizontal
LCL-LCS-DTL-374QT
355
20
<1
<5
>100:1, horizontal
LCL-GARNET
355
20
0.4
< 1.7
>100:1, horizontal
Model name
Diode pumped solid state lasers for microscopes
Product Specialists Bernhard Dauner +49 8153 405-17 b.dauner@laser2000.de Dr. Stefan Kremser +49 (0) 8153-405-16 s.kremser@laser2000.de
Specifications
DPS-Series 3500
Power at 355nm
0.1W - 2W
Pulse Repetition Rate
20 - 150 kHz
Pulse width (nominal)
20 - 100 nsec
Wavelength
354.7 nm
Mode (M²) Specification
TEMoo(<1.25)
Beam Diameter (1/e²)
1.5 mm
Pulse to Pulse Stability
< 10%
Power Stability (8 hr. drift at constant temp.)
<5%
Beam Pointing Stability at constant temp.
<50 urad
Polarization (Linear, Vertical)
>100:1
Beam Divergence (full angle)
< 0.5 mrad
Input voltage
90-240 VAC
Power consumption, maximum
500 W
Ambient operating temperature (non-condensing)
10-30 °C
Laser head dimensions (LWH)
20.0 x 7.5 x 6.5 in.
Laser head weight
30 lbs.
Laser power supply dimensions (LWH)
12.2 x 13.5 x 5.4 in.
Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
www.laser2000.de
51
Deep UV Gas Lasers Deep UV Gas Lasers Ultra-easy ultraviolet
Series 30 laser with integrated controller Series 70 laser with integrated controller
Make ultra-sensitive measurements of Raleigh, Raman, fluorescence or phosphorescence emissions generated by deep UV excitation. Our “instrument solutions” combine a deep UV laser source with an array of analyzer and detector plugand-play modules. Detection choices include single and multichannel PMT and photodiode detector modules that are gated in synchronism with the laser and offer flexible boxcar integration and averaging for enhanced signal-to-noise data collection. Analyzer options include UV Raman, laser induced native fluorescence, CE and HPLC modules. The combination of plug-and-play source, analyzer and detector modules enables you to rapidly develop breadboard and prototype instruments for a wide range of applications from research to product analysis to environmental monitoring with data sampling rates up to 20 Hz.
Flexible for the lab, made for the real world
224 and 248 nm Wavelengths 224 nm, 248 nm Narrow line width <0.5 pm (3 GHz, 0.1 cm-1) Innovative plug- and play instrument solutions Rugged design for reliability USB or Ethernet interface with LabView Built-in laser power monitor Wide operating environment (-200 – 100 °C) Instant on (< 10 µs from cold start) 90 VAC – 240 VAC input at < 10 W, no water cooling or toxic
An array of accessories such as emission line purity modules and fiberoptic couplers enable you to mate our components with a wide range of devices from third-party suppliers. Communication with the laser and all plug-and-play modules is accomplished via USB or Ethernet using LabView drivers. Our „instrument solutions” provide a seamless fit for many applications such as laser induced native fluorescence or UV resonance Raman analyzers, photoluminescence, capillary electrophoresis, high performance liquid chromatography, phosphorescence and many other types of instruments. Rugged design, reliable performance and low cost make them ideal for the field researcher and the OEM.
chemicals
Deep ultraviolet for less The DUV family of lasers offer 224.3 nm and 248.6 nm for fraction of the cost of the competition. The laser is the size, weight and power consumption of a HeNe laser but with output in the deep UV. The self-contained, integrated, laser controller enables remote computer control for ease of operation and flexible data collection via LabView software. With an input power less than 10 W the need for water cooling and other thermal management issues is eliminated. The lasers reach full power in less than 20 microseconds from a cold start from any ambient temperature from -200 to 100 °C without preheating or temperature regulation. With output over 100mW and linewidths less than 3GHz or 0.0005nm these are great sources for a wide range of applications.
52
Deep UV laser, plasma line filter, cuvette holder, PMT adapter and 1/2” filter adapter all interconnect with standard optics and Hamamatsu PMTs to enable a complete system
Call us: +49 (0)8153 405-0
Deep UV Gas Lasers Digital Detector Controllers The tiny „instruments on a chip” are key to the Deep UV laser systems. They include multichannel digital photo multiplier tube controllers with gated boxcar integrators. This accessory is a direct plug-in unit to the lasers and enables ultra-sensitive detection simultaneously in ten or more channels automatically synchronized with the deep UV laser. We also offer plasma line cleaning optics modules, fiber optic interfaces and many more accessories. Together they form a catalog of options from which a researcher can choose to develop his own tools for sensitive instrument. Digital Detector Controller
HeAg lasers @ 224.3 nm Model Peak power(quasi cw) System dimensions
PSY-HeAg70-224SL
PSY-HeAg30-224SL
> 50 mW
> 10 mW
10 cm x 10 cm x 70 cm
5 cm x 13 cm x 30 cm
System weight
3.6 kg
1.4 kg
Pulse frequency
0 - 20 Hz
0 - 5 Hz
Longitudinal mode spacing
257 MHz
Pulse width
642 MHz 20 - 120 µs, adjustable
Pulse synchronism
internal or external
Beam diameter
3 mm
Beam divergence Oscillation bandwidth Power consumption Line requirements
< 4 mrad < 0.5 pm, < 3 GHz, < 0.10 cm -1 < 10 W 90 - 250 VAC, 47 - 63 Hz oder 24 VDC
NeCu lasers @ 248.6 nm Model Peak power(quasi cw) System dimensions
PSY-NeCu30-248SL
> 250 mW
> 50 mW
10 cm x 10 cm x 70 cm
5 cm x 13 cm x 30 cm
System weight
3.6 kg
1.4 kg
Pulse frequency
0 - 20 Hz
0 -5 Hz
Longitudinal mode spacing
257 MHz
Pulse width Pulse synchronism Beam diameter Beam divergence Oscillation bandwidth
Laser Line Filter System (PLRFS) for Deep UV Laser
PSY-NeCu70-248SL
Power consumption Line requirements
642 MHz 20 - 80 µs, adjustable internal or external 3 mm < 4 mrad
< 0.5 pm, < 3 GHz, < 0.10 cm -1 < 10 W 90 - 250 VAC, 47 - 63 Hz oder 24 VDC
Product Specialists Dr. Stefan Kremser +49 (0) 8153-405-16 s.kremser@laser2000.de
Digital Detector Controller
Bernhard Dauner +49 8153 405-17 b.dauner@laser2000.de
Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
www.laser2000.de
53
l
Photon Counting Silicon Solutions for Low Light Detection We offer a range of innovative silicon-based low-light imaging and photon counting sensors, arrays and modules. Products include photon-counting detectors and large-area high-gain APD.
PCDMini
Miniature Photon Counting Device Version 1 Revision 3.0
APD in Geiger Mode Large Area High Gain APD Time binning 2.5 nanoseconds Highest QE reached by back thinning
This unique silicon photon counter is based on a new shallow junction Geiger-mode avalanche photodiode technology. 1. Quantum Efficiency SensL‘s products and services provide a revolutionary alter45 native to Units the PMT Photomultiplier Tube, the existing standard Maximum Notes for photon counting applications. Compatible and adaptable, are ideal for applications such as30biosensors, 950SensL‘s products nm QE (%) quantum cryptography, medical diagnostics, laboratory instrumentation, environmental monitoring and space exploration. Pm 15
Typical
Pm main Pm
markets applications are: Via lensand attachment
140 PhotonpsCorrelation
0
Spectroscopy
Confocal ps Microscopy 10kcps to 1Mcps Fluorescence Lifetime Measurement 100 cps Min dark count @ -20°C Biological Sensors % Max QE @ -20°C, Microarray Scanning 550nm – 650nm DNA Biochips/Sequencing 0.30 % Bio/Chemical Sensors ns Scientific Instrumentation Version 1 Revision 3.0 Proteomics/Protein Biochips V 50: load Flow Cytometry ns Capillary Electrophoresis Mcps Continuous light Nuclear Medicine (PET/SPECT Scanning)
PCDMini
oton Counting Device
e
Photon counting
V
@50mA without cooling
V V V
@10mA @10mA Dependent on APD breakdown voltage
s
-20°C
1. Quantum Efficiency @200mA with cooling 45
13 16 40
30 QE (%)
400
600
800
Wavelength (nm)
2. Jitter 50k
Count (cps)
130ps
0 20.0
20.5 Time (ns)
21.0
3. Time-walk 50k 1Mcps
Count (cps)
50ps
15
0
High gain sensing
1000
10kcps 0 400
600
800
1000
20.0
20.5 Time (ns)
Wavelength (nm)
2. Maximum Jitter 6. Count Rate
21.0
4. Afterpulsing
50k
0.15
Product Specialist
Count (cps)
130ps
Dr. Helge Brüggemann +49 (0) 30 962 778-12 h.brueggemann@laser2000.de
Afterpulsing Probability (%)
Sales Assistance 0 20.0
3. Time-walk
20.5 Time (ns)
21.0
0 0.1
House, Riverview Business Park, Bessboro Road, Blackrock, Cork, Ireland Call us: 14 350 54 442,50k Fax. +353 214 350 447, sales@SensL.com, www.SensL.com
1
10 100 Time (Ps)
1000
+49 (0)8153 405-0
Gabriela Thunig +49 (0) 8153-405-43 g.thunig@laser2000.de
Spectroscopy Cameras
1024 x 256 imaging pixels; 26 x 26-um pixels; 100% fill factor; 26.6 x 6.7-mm imaging area
rms @ scan rate
Minimum
Typical
CCD Image sensor Maximum
Marconi CCD30-11; scientific grade 1; AIMO; MPP back illuminated, anti-reflecti
7
10
CCD fomat
1024 x 256 imaging pixels; 26 x 26-um pixels; 100% fill factor; 26.6 x 6.7-mm im
20
System read noise e- rms @ scan rate
Minimum
17 25
30
apacity
Typical
Maximum
100 kHz
7
10
1 MHz
17
20
UV/VIS/NIR Image intensified Spectroscopy Cameras 250 ke-
500 ke-
600 ke-
1 Me-
Key Features
2 MHz
single pixel 7k
High QE between 950-1650 nm -40 deg. C -50 deg. C <1 e- rms read noise see QE curve 16 bit dynamic 200 range 1024 X 256 Pixel Format 1.1 10 usec 26 µm pixel pitch 30 Hz -40 °C TE Cooling 4 e-/count, 8 e-/count, 16 e-/count, USB 2.0 data port
18k
ode Cooling Temperature
or
h
30
Spectrometric well capacity
current e-/pixel/sec @ -40C
on
25
250 ke-
500 ke-
600950 ke- is ideal 1 Melogy, the MOSIR for low light spectroscopy and Dark current e-/p/hr imaging applications including Raman spectroscopy, chemical -40 deg C operation 7 18 imaging, astronomy, and photoluminescence. The combinatiMaximum Cooling Temperature on of high QE and low noise gain between 950 and 1650 nm TE Cooling -60 -70 than CCD‘s or InGaAs arrays. provides far superior sensitivity binned
Software-selectable gains
4 e-/count, 8 e-/count, 16 e-/count,
Dynamic range
16 bitsplane focal
The MOSIR array has an ideal 4:1 aspect ratio <2% spectroscopy format (1024X256, 26X26ìm pixel pitch), 16 bit Vertical shift time us 16 bits dynamic range,30USB 2.0 data port and interfaces to leading Spectral rate, Hz, full vertical binning <2% External controller NOT required The MOSIR™ 950 stands spectrometers and microscopes. Dark charge is minimized for 100 kHz digitization 50 l vertical binning alone as the world‘s first and exclusive NIR image intensified all sensors with a proprietary thermo-electric cooling and va1 MHz digitization 180 on 50 spectroscopy camera between 950 and 1650 nm. cuum design. The MOSIR camera is extremely compact and Sensor cooling range +20 deg C to -40 deg C n 180 does not require an external controller. Temperature stability +/-0.1 deg C over entire temperature range; +20 deg C to -40 deg C Based on Intevac‘s patented and exclusive transfer electron +/-0.1 deg C over entire temperature range; (TE) photocathode and electron bombarded (EB) gain technoRate
ains
emperature
Nonlinearity
0 deg C to +30 deg C
MOSIRTM 950 TE Photocathode QE Curve
Typical QE Response for Curve 30-11 (BBAR and UVAR) MOSIRTM 350/150 QE 90 30-11-BBAR 30-11-UVAR
Quantum Efficiency (%)
80 70 60 50 40 30 20 10 0 200
900
300
400
500
600
700
800
900
1000
1100
Wavelength (nm)
MOSIR 950 SPECIFICATIONS TM
Sensor fomat
256 imaging pixels; 26 x 26-um pixels; 100% fill factor; 26.6 x 6.7-mm imaging area INTEVAC, INC. 3560 Bassett Street1024 Santax Clara, CA 95054-2704 Fax 408-727-5739 www.mosir950.com/ System read noise Phone e- rms 408-588-2150 @ scan rate Minimum Typical Maximum 100 kHz 1 MHz
NOTE: This product is under the export control of the Office of Defense Trade Controls, US Department of State, and is subject to the 7 10 the knowledge and consent of the International Traffic in Arms Regulations. Transshipment to any destination outside the United States without Office of Defense Trade Controls is strictly prohibited. The information and specifications included in this datasheet are preliminary17 and subject to change.
2 MHz
25
20 30
Spectrometric well capacity
INTEVAC, INC. 3560 Bassett Street Santa Clara, CA 95054-2704 Phone 408-588-2150 Fax 408-727-5739 www.mosir950.com/ The information and specifications included in this datasheet are preliminary and subject to change.
single pixel
250 ke-
500 ke-
binned
600 ke-
1 Me-
Photocathode Dark current e-/pixel/sec @ -40C -40 deg C operation Minimum Photocathode Cooling Temperature
7k -40 deg. C
Spectral Response
see QE curve
EB Gain
200
Excessive Noise Factor
1.1
Minimum Gate Width
10 usec
Maximum Repetition Rate
30 Hz
18k
-50 deg. C
Software-selectable gains
4 e-/count, 8 e-/count, 16 e-/count,
Dynamic range
16 bits
Nonlinearity
<2%
Spectral rate, Hz, full vertical binning 100 kHz digitization 1 MHz digitization
50 180
Sensor cooling range
+20 deg C to -40 deg C
Temperature stability
+/-0.1 deg C over entire temperature range;
Ambient Operating Temperature
0 deg C to +30 deg C
www.laser2000.de
55
Spectrometer NIR InGaAs Spectrometers Our high performance NIR InGaAs spectrometers use a linear photo diode array detector with 512 pixels (1024 optional) to provide maximum sensitivity. The NIR InGaAs spectrometers use single strand SMA-905 fiber optic input. Several models provide a variety of operational ranges and resolutions suitable for both spectroscopy and optical spectrum analysis. NIR applicawww.StellarNet-Inc.com tions include chemical ID and moisture analysis, SpectroRadiometry and optical power measurements, laser chaSurf microsensor the Newapplications, Wave in Portable Fiber Optic racterization, and thicker thin-film measurements.
Analytical Instrumentation
Spectrometry
EPP2000-NIR-InGaAs Spectrometer - for Near Infrared Applications StellarNets’ high performance InGaAs spectrometers cover the NIR wavelength range from 0.9-2.2um. The units are exceptionally 4000:1 with 5 decades Dynamic Range: robust with no moving parts and are packaged up to < 1 nm resolving res. with 25um slit Optical Resolution: in small rugged metal enclosures (2.75 x4 x6 512 or 1024 pixel InGaAs PDA Detector Type: inch) for portable, process, and lab applications. The InGaAs detector is a Sensors 900 - 1700 nm Detector Range: Unlimited linear photo diode array with 512 25 µm x 500 µm Pixel Size: pixels (1024 optional) 25um by 500um tall to 4000:1 with TEC cooling Signal to Noise: provide maximum sensitivity. The detector 14-bit @ 2.5 MHz rate Digitizer: has an integrated thermo electric cooler (TEC) maintained at –10 qC, stabilized within +/-0.1 qC. The EPP2000-NIR-InGaAs spectrometers use single strand SMA 905 fiber optic input. Several models provide a variety of operational ranges and resolutions suitable for both spectroscopy and optical spectrum analysis.
Dimensions:
150 x 100 x 70 mm
Power Consumption:
2 A @ 5 VDC
Interface:
USB-2 or parallel
Data Transfer Speed:
40x faster than USB-1
Detector Integration:
1 ms to 30 s
Slit Size Options:
25, 50, 100, or 200 µm
Fiber Optic Input:
SMA-905 0.22 NA single fiber
Operating Systems:
Win 9x/NT/00/XP
Software Included:
SpectraWiz program & apps
Also free programs for:
LabView / VC / VBA / Delphi
NIR applications include chemical ID and moisture analysis, and The SpectraWiz softwareSpectroRadiometry is included to optical power measurements, laser accurately measure wavelength emischaracterization, microsensor abapplications, sions, reflectance, transmission, and thicker thin-film measurements. sorption, concentrations, and absolute intensities.
The SpectraWiz software is included for Win9x/Me/NT/2000/XP to accurately measure wavelength emissions, reflectance, transmission, absorption, and absolute intensities. Driver and customizable programs are also included for operation in LabVIEW, Excel+VBA, VC, and Delphi. The system includes high speed plug & play interfaces for notebook and desktop computers. The USB2EPP cable connects to Product Specialist USB-2 ports (40x faster than Dr.USB1). Helge Brüggemann
Specifications
Dynamic range: Resolving resolution: InGaAs Detector: Detector range: Pixel size: Pixel well depth: Selectable well control: 56 Signal to noise: Digitizer:
+49 (0) 30 962 778-12
Zero defect 512 detector EPP2000-NIR-InGaAs Spectrometer $13,125 h.brueggemann@laser2000.de 4000:1 with 5 decades 3.1nm with 25um slit 512 pixel cooled PDA array 0.9-1.7um (900-1700nm) 25um x 500um 130 x10^8 electrons 130 x10^8 or 5 x10^6 el. Call 4000:1 with TEC cooling 14 bit @ 2.5 MHz rate
Dimensions: 150 x 100 x 68.8 mm Power consumption: 2 Amps 5 VDC @ Sales Assistance Interface: USB-2 and Parallel Gabriela Thunig Data transfer speed: 40x faster than USB-1 +49 (0) 8153-405-43 Detector Integration: 1 millisecond to 30 secs g.thunig@laser2000.de Slit size options: 25, 50,100, or 200um Operating systems: Win98/NT/Me/00/XP us: +49 (0)8153 Software included:405-0 SpectraWiz program & apps Also free programs for: LabView,Excel+VBA,Delphi
Spectrometer UV-VIS Spectrometers The EPP2000-C-UV/VIS spectrometers utilize a 40 mm diameter concave grating with aberration correction to provide superb imaging. This significantly improves spectral shapes by eliminating coma and stigmatism found in plane grating spectrograph designs. The flat field spectrograph architecture does not utilize mirrors, and therefore provides the lowest possible stray light in the UV with additional Assistance from the holographic line grating. A multi-band filter is integrated into the spectrograph to provide order sorting and prevent optical aliasing. The optical signal is input via single strand fiber optic cable into a standard SMA-905 connector. The SpectraWiz software is included to accurately measure wavelength emissions, reflectance, transmission, absorption, concentrations, and absolute intensities.
Dynamic Range:
2000:1 with 6 decades
Dimensions:
150 x 100 x 70 mm
Optical Resolution:
< 1 nm Resolving Res. with 25um slit
Power Consumption:
100 mA @ 5 VDC
Detector Type:
2048 pixel CCD, PDA opt.
Interface:
USB-2 or parallel
Detector Range:
190 - 850 nm or 250 - 885 nm
Data Transfer Speed:
3x / 40x faster than USB-1
Pixel Size:
14 µm x 200 µm
Detector Integration:
2 ms / 1 ms [12 / 14-bit] to 65 s
Concave Grating:
Aberration corrected
Slit Size Options:
5, 10, 25, 50, 100, or 200 um
Grating Type:
Holographic, 590 g/mm
Stray Light:
0.02% @ 435 nm; 0.2% @ 200 nm
Spectrograph:
f/2, Flat field - No mirrors
Fiber Optic Input:
SMA-905 0.22 NA single fiber
Order Sorting Filter:
Integrated multi-band
Operating Systems:
Win 9x/NT/00/XP
Signal to Noise:
1000:1 CCD, PDA 2000:1
Software Included:
SpectraWiz program & apps
Digitizer:
12-bit, 14-bit optional
Also free programs for:
LabView / VC / VBA / Delphi
Product Specialist Dr. Helge Brüggemann +49 (0) 30 962 778-12 h.brueggemann@laser2000.de
Sales Assistance Gabriela Thunig +49 (0) 8153-405-43 g.thunig@laser2000.de
www.laser2000.de
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Broad band light source Spectrally Programmable Light Engine If you could instantly control the spectrum, intensity and timing of your light source, what could you achieve? Replacing light created by a frustrating assemblage of mechanical devices, OneLight Spectra improves illumination systems using a patented software-driven light engine – or Digital Light Source – to achieve an entirely new category of illumination. Employing simple-yet-powerful software, precise spectral profiles are created. Illumination, intensity and exposure duration are under your complete control. And the result is nothing short of evolutionary.
THE EVOLUTION OF SPECTRAL CONTROL SPLE technology marks a major departure from conventional light solutions. It allows for any desired mix of wavelengths (color) and intensity of illumination to be dynamically configured and instantly selected under software control to produce currently unattainable contrast, intensity, purity and range of color. Able to respond faster than most cameras capture images, OneLight Spectra can improve the performance of most microscopy or spectroscopy imaging systems. Fully programmable, the range of wavelengths you can use is no longer defined by how many band-pass or neutral density filters your filter wheel can accommodate, or how fast you can change them. Wavelength requirements are defined using an intuitive software application, instead of the coating laboratory. In this sense, OneLight Spectra is like having a filter factory right at your fingertips, with none of the limitations.
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SOFTWARE CONTROL OneLight Spectra comes with an intuitive software interface that provides easy command of the most commonly used functions of the instrument. Users can set up and save their own particular instrument configurations; recalling them quickly and easily to continue an experiment, without having to repeat the setup procedure. You can save and load any spectral profiles you create, as well as those from the libraries of spectral profiles that are provided to get you started. The software is organized around a modular plug-in architecture, making it easy to add new features by simply adding new plug-ins. For example, the Tuner plug-in provides selection of wavelength bands or “filter functions” that are the digital light equivalent to short-pass, long-pass, band-pass or notch filters, and are just a few mouse clicks away. Freeform is another plug-in that provides a graphic equalizertype application for the construction of arbitrary spectral profiles on the fly. You can also construct spectral profiles from data you have measured, created or captured in any other manner. For those who want to add functionality and develop software for their own applications, we provide an optional SDK that gives programmatic access to all the functions available. Simply put, creating light has never been this easy. And once you have experienced the simplicity and flexibility with which you can create light using OneLight Spectra’s software, you will never want to do it any other way.
Call us: +49 (0)8153 405-0
Broad band light source
ď&#x20AC;´Product Specialists Bernhard Dauner +49 (0) 8153-405-17 b.dauner@laser2000.de
ď&#x20AC;´Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
www.laser2000.de
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Optics & Optomechanics Optics and Optomechanics The full range of available products is offered... Optomechanics:
optical mounts stages (translation, rotation, goniometer) motorized mounts and stages mounting systems OEM solutions
Optics:
Please contact Victoria Benedikt for catalogues:
a wide variety of optics (mirror, filter, lenses, etc.) optical coatings
+49 (0) 8153-405-61 v.benedikt@laser2000.de
Product Specialists Bernhard Dauner +49 (0) 8153-405-17 b.dauner@laser2000.de Dr. Andreas Stangassinger +49 (0) 8153-405-40 a.stangassinger@laser2000.de
Sales Assistance Victoria Benedikt +49 (0) 8153-405-61 v.benedikt@laser2000.de
Laser Safety Goggles Full spectrum Polycarbonate laser protection
Isabell Langfellner +49 (0) 8153-405-26 i.langfellner@laser2000.de
Laser goggles are provided by companies with more than 30 years experience in the field of eye safety for medical and industrial applications. Filters for wavelength regions between the UV to IR (190 nm-1700 nm) are available and protect against laser powers up to 100W (cw). Also goggles for short pulse lasers as femtosecond- or picosecond lasers are certified. The light and very robust material together with a variety of frames allows the user to find the right goggle for comfortable usage.
Webcode: 3001
For full information use the Laser 2000 Website (www.laser2000.de). All laser goggles shown are fully CE and EN207 certified. As registered user you will also be able to download a program to easily identify the necessary filter for your application. If you still have questions please contact our product specialists.
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Call us: +49 (0)8153 405-0
Deep UV Gas Lasers Find all these products and many more on our web page www.laser2000.com: • Fiber Optics • Network Technology • Digital Testing • Lasers & Lightsources • Ophthalmic Solutions • Laserprotection • Optics & Optomechanics • UV Technology • Infrared Technology • Optical Instrumentation • Image Processing
Free catalogues! Webcode: Mail order for your catalog Fax +49 8153 405-33
9001
c Fiber Optics, 240 pages, in German c Network Solutions, 16 pages, in German c FO Components & Lab Equipment, 40 pages c Optical Filters for Life Sciences, 62 pages c Lasers and Lightsources, 124 pages, in German c Optical Instrumentation, 104 pages, in German c Laser Safety, 24 pages, in German c Image Processing, 72 pages, in German c Infrared Technology, 48 pages, in German c Ophthalmic Systems, 12 pages c
lease keep me informed by eMail. P I am particularly interested in:
c Fiber Optics c Network Technology c Digital Testing c Lasers & Lightsources c
c Ophthalmic Solutions c Laserprotection c Optics & Optomechanics c UV Technology
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www.laser2000.de
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Find all these products and many more on our web page: • Fiber Optics • Network Technology • Digital Testing • Lasers & Lightsources • Ophthalmic Solutions • Laserprotection • Optics & Optomechanics • UV Technology • Infrared Technology • Optical Instrumentation • Image Processing
www.laser2000.com
Laser 2000 GmbH Argelsrieder Feld 14 DE-82234 Wessling Munich/Germany Tel. +49 8153 405-0 Fax +49 8153 405-33 info@laser2000.de Office Berlin Pasedagplatz 3-4 DE-13088 Berlin Tel. +49 30 962778-0 Fax +49 30 962778-29 info@laser2000.de
Office Dresden Geschwister-Scholl-Str. 47 DE-01877 Bischofswerda Tel. +49 3594 705980 Fax +49 3594 705985 info@laser2000.de
Office Mönchengladbach Ohlerkamp 4 DE-41069 Mönchengladbach Tel. +49 2161 307300 Fax +49 2161 307310 info@laser2000.de
Office Bamberg Hängbergstrasse 18 DE-96199 Zapfendorf Tel. +49 9547 870369 Fax +49 9547 871281 info@laser2000.de
U.K. Belgium Netherlands Sweden
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sales@laser2000.co.uk sales@laser2000.be info@laser2000.nl info@laser2000.se
+44 1933 461666 +32 71 610 640 +31 297 266 191 +46 11 369 681
+44 1933 461699 +32 71 610 649 +31 297 266 134 +46 11 369 689
As part of our continuous program for product improvement, Laser 2000 reserves the right to change specifications without notice. Copyright © 2009 Laser 2000 GmbH. All Trademarks are the registered property of their respective owners.
! New office
Office Wien Christian Schöbel AT-1160 Wien Tel. +43 1 4810498 Fax +43 1 4810548 info@laser2000.at
www.laser2000.com