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INSTRUMENTAL ANALYSIS ON PHARMACY
SHIVAM DUBEY BPYN1PY18041 MCQ’S
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1. How is molar concentration of solute in stationary phase related to molar concentration of solute in mobile phase?
a) Directly proportional b) Inversely proportional c) Equal d) Not related
2. If the value of the distribution constant ‘k’ is one, then what could be inferred about the distribution of solute?
a) Its distribution in stationary phase is greater b) Its distribution in mobile phase is greater c) It is equally distributed in stationary and mobile phase d) It is distributed in a random manner
3. The time taken by the analyte after sample injection to reach the detector is called _________
a) Dead time b) Solute migration rate c) Adjusted retention time d) Retention time
4. The time required for a molecule of the mobile phase to pass through the column is called ___________
a) Dead time b) Solute migration rate c) Adjusted retention time
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d) Retention time
5. Adjusted retention time is the remaining retention time after subtracting __________ from ___________
a) Solute migration rate and retention time b) Retention time and solute migration rate c) Dead time and retention time d) Retention time and dead time
6. Which of the following is the volume of mobile phase required to make a solute band move from the point of injection through the column to the detector?
a) Dead volume b) Retention volume c) Void volume d) Adjusted retention volume
7. Adjusted retention volume is the remaining retention volume after subtracting ___________ from _____________
a) Solute migration rate and retention volume b) Retention volume and solute migration rate c) Dead volume and retention volume d) Retention volume and dead volume
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8. Which of the following is defined as the ratio of moles of solute in stationary phase to the moles of solute in mobile phase?
a) Distribution constant b) Volumetric phase ratio c) Retention factor d) Total porosity
9. Which of the following is the ratio of interstitial volume of packing to the volume of its total mass?
a) Distribution constant b) Volumetric phase ratio c) Retention factor d) Total porosity
10. Which of the following is the ratio of length of column packing to dead time?
a) Average linear rate of solute migration b) Average linear rate of mobile migration c) Relative migration rate d) Selectivity factor
11. Which of the following is the ratio of length of column packing to retention time?
a) Average linear rate of solute migration b) Average linear rate of mobile migration c) Relative migration rate
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d) Selectivity factor
12. Retention distance is the distance between point of injection and minimum peak in the recorder or computer generated chart.
a) True b) False
13. Retention volume can be obtained by finding the product of which of the following parameters?
a) Dead time and total porosity b) Retention time and volumetric flow rate c) Adjusted retention time and volumetric flow rate d) Retention time and total porosity.
14. Retention factor is also known as capacitance factor. a) True b) False
15. What must be the value of selectivity factor? a) Equal to 1 b) Less than 1 c) Greater than 1 d) Greater than 0
1. Which of the following is the disadvantage of reciprocating pump used in liquid chromatography?
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a) Produces pulsed flow b) Corrosive components c) Does not have small hold-up value d) Does not have moderate flow rate
16. Which of the following is not a disadvantage of Pneumatic pumps used in liquid chromatography?
a) Pulsed output b) Dependent on solvent viscosity c) Dependent on back pressure d) Inconvenient for solvent gradient elution
17. Which of the following is not a desired characteristic of pulse dampers or flow smootheners used in liquid chromatography?
a) Easy mobile phase change over b) Constant flow must be maintained c) Should be effective at low system pressure d) Maximal dead volume
18. Which of the following will improve the efficiency of separation process in liquid chromatography? a) Increase in sample size, increase in column diameter b) Reduction in sample size, increase in column diameter c) Increase in sample size, reduction in column diameter d) Reduction in sample size, reduction in column diameter
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19. Which of the following are the practical problems that arise due to the decrease in column diameter? a) Requirement of large particle size and high pressure drop b) Requirement of large particle size and low pressure drop c) Requirement of small particle size and high pressure drop d) Requirement of small particle size and low pressure drop
20. Which of the following is not true about guard column used in liquid chromatography?
a) It filters particles that clog the separation column b) It extends the lifetime of separation column c) It allows particles that cause precipitation upon contact with stationary or mobile phase d) The size of packing varies with the type of protection needed
21. Which of the following columns are not used in liquid or high performance liquid chromatography?
a) Analytical column b) Separation column c) Guard column d) Capillary column
22. Which of the following is not a Column-type Liquid chromatography?
a) Gel permeation b) Ion exchange c) Liquid-solid
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d) Paper
23. Which of the following is not true about radial compression column when compared to standard separation column?
a) Internal diameter decreases b) Overall operating pressure decreases c) Analysis time decreases d) Solvent flow increases
24. Which of the following is not true about narrow bore column when compared to standard columns?
a) Internal diameter decreases b) Volumetric flow decreases c) Solvent cost is saved d) Detector response time increases
25. Which of the following types of liquid chromatography uses immobilized biochemical as stationary phase?
a) Ion exchange chromatography b) Exclusion chromatography c) Affinity chromatography d) Gel permeation chromatography
26. What is the drawback that occurs in using ion exchange chromatography on sulphonated polystyrene resin and colourimetry for amino-acid analysis?
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a) Less accuracy b) Low resolution c) Inconvenient to handle many individual samples d) Slow in operation
27. Which one of the following methods is the most suitable for aminoacid analysis?
a) Gas chromatography b) Ion exchange chromatography c) Paper electrophoresis d) Resin column chromatography
28. Which of the following colour reagents are used in Resin column chromatography?
a) Marquis reagent b) Benedict reagent c) Ninhydrin d) Nessler’s reagent
29. Which of the following amino-acids is measured at a wavelength of 440nm using photometric systems?
a) Proline b) Alanine c) Glutamine d) Valine
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30. In Automatic amino-acid analyzer, the sample containing ___________ of each amino compound is introduced at the top of the ion exchange column.
a) 1 to 10 µmoles b) 1 to 10 moles c) 0.05 to 2 moles d) 0.05 to 2 µmoles
31. Which of the following cannot be analysed using resin column chromatography?
a) Peptides b) Amines c) Amino compounds d) Components which are ninhydrin negative
REFERENCES https://instrumentationtools.com/top-1000-analyticalinstrumentation-questions-answers/ https://www.sanfoundry.com/1000-analyticalinstrumentation-questions-answers/ http://mcet.in/wpcontent/uploads/EIE/2020/EIE_QB/Analytical%20Instrume ntation.pdf https://s3.wp.wsu.edu/uploads/sites/9/2015/03/S14_Chem 425.pdf https://www.youtube.com/watch?v=mb4oK1N3Gxk