Metabolism of Fat

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FAT


How fat can be good...... Energy source Transporter for lipid soluble vitamin Cell membrane structure Precursor for Vit D and steroid hormoneÂ


Properties of Fat Water insoluble Can be solid or liquid at room temperature Energy dense (9 kcal/g) Triglyceride is the main form of fat in bodyÂ


Types of Fat Triglyceride Triglyceride = 3Fatty Acid + 1 glycerol Main form of fat in body

Phospholipid Phospholipid = 2Fatty Acid + 1 glycerol + Phosphorus compound Cell membrane Â

Cholesterol Found in animal source food Produced by the liver Produce hormone


Good Fat vs Bad Fat


Bad Fat

Good Fat


Essential Fatty Acid Omega-3

Vege Oil

Omega-6


Fat Digestion & Absorption Mouth

Mix with lingual lipase become lipid droplet

Stomach

Mix with gactric lipase. Trigleceride -> Diglyceride

Small Intestine

Mix with pancreatic lipase + bile. Tri/Diglyceride ->Â Free Fatty acid

Mouth

Esophagus

Stomach

Absorption (Chylomicron)

Large Intestine

Large Intestine


Fat Transport

High Density Lipoprotein (Good Cholesterol)

Low Density Lipoprotein (Bad Cholesterol)

Chylomicron


Fat Metabolism


FAT tRANSPORT Triglyceride = Fatty Acid + GlycerolÂ

Lipolysis

Carnitine

Glycerol Mitochondria

Lipase Enzyme Adipocyte

Free Fatty Acid

Cell

Glycerol Free Fatty Acid

+

Albumin

+

Beta-Oxidation Kreb Cycle ETC


Lipolysis Breakdown of fat stored in adipocyte Triglyceride -> Free fatty acid + Glycerol Produce ketone High ketone -> ketosis Ketone -> Glucose (Gluconeogenesis) Hormones that enhance lipolysis: Cortisol, Growth homone, Epinephrine, Norepinephrine Glycerol Lipase Enzyme Triglyceride Lipolysis

Free Fatty Acid


Beta-Oxidation Catabolic process Fatty acid are degraded by removal of 2-C unit Occurs in the mitochondria Produce Acetyl-CoA + NADH + FADH2 4 steps : Dehydrogenation, hydration, oxydation, thiolysis


ATP Production from BetaOxidation 7n - 6

n = number of carbon of fatty acid ATP C16:0 fatty acids = 7(16) – 6 = 112 – 6 = 106

Every double bond present must deduct 2 ATP. ATP C18:3 fatty acids = 7(18) – 6 – 2 – 2 – 2 = 114


Kreb Cycle Acetyl-CoA combines with oxaloacetate to form citrate Citrate is broken down to oxaloacetate again in 1 cycle Product :Â CO2, NADH, FADH, GTP (ATP) Occur in mitochondria


Electron Transport Chain (ETC) Primary site for ATP synthesis (aerobic) Contain of 3 protein complex and 2 electron carriers Convert NADH & FADH2 -> ATP Product : ATP, H2O Occur in mitochondria


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