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Sequencing The SPRIselect from Beckman Coulter Life Sciences utilizes SPRI-based (solid phase reversible immobilization) chemistry to speed and simplify genomic DNA size selection for next generation sequencing fragment library preparation. Following shearing, the library construction process requires size
selection to produce uniform distribution of fragments. SPRIselect allows size distribution to be adjusted between 150 and 800 base pairs to suit the application and sequencing platform. The process can be performed manually or can be automated for high throughput in 96-well plates on platforms such as Beckman Coulter Life Sciences’ Biomek workstations. SPRIselect reagent kits are available in 5, 60 and 450 mL volume, and come with guidelines to assist users in customizing protocols. Gel cartridges, chips and additional instruments are not needed. Web: www.beckmancoulter.com
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Refrigerated incubator The new
Binder Inc. KT 115 refrigerated incubator utilizes Peltier refrigeration technology to provide secure, accurate control. With comprehensive program functions, the unit offers a wide range of capabilities for incubating biological materials. Functional chamber volume is 3.7 cu.ft. (104 litres), and the unit can run at temperatures down to 4°C. The incubator’s APT.lineTM preheating chamber technology ensures homogenous temperature conditions, as well as rapid heating and recovery times. Horizontal airflow from both sides of the chamber provides uniform temperature distribution, even when the unit is fully loaded. Temperature, set digitally, is accurate to a tenth of a degree and ensures effective and gentle incubation. State-of-the-art rotary pushbutton input and graphic LCD display are used to easily set the most demanding programs in English, French or Spanish. The controller accommodates up to 10 different users, each having up to 10 stored programs. The controller’s USB interface can be used to transfer data to a PC for BINDER APT-COM™ software analysis. Web: www.binder-world.com
Chromatography GE Healthcare’s ÄKTA™ pure, is a chromatography system for purification of proteins. ÄKTA pure is easy-to-use and can support both routine and challenging purification requirements, as well as being adaptable to changing research needs. ÄKTA pure is specifically designed to enable researchers - from beginners to experts - to easily isolate their target protein, whether under simple or complex conditions. With the addition of simple drag-and-drop method templates in the UNICORN™ 6 control software it is also possible to electronically track the use of, and data obtained from, individual columns, which is particularly useful as chromatography systems are often placed in shared facilities. Web: www.gelifesciences.com/akta
Counter The Scienceware® Colony Counter is a simple to operate device that can count colonies of bacteria or other microorganisms grown on an agar plate.
It marks, counts, and gives an audible and visual alert each time a count is registered on the digital display. Its lightweight, comfortable to hold, pen-style design reduces the likelihood of error, combining all aspects of counting into one motion. Error is further reduced by the ability to count both up and down to verify sample counts. Able to store up to 35 sample counts in its memory, it can also display the sum total of all colonies counted. The display has a backlight for easy viewing in low light conditions; the audible alert can be turned off if desired; and the counting function can be locked to prevent error during breaks between counting. Web: www.belart.com
Gas controller Warner Instruments introduces its Bold Line CO2 Controller, part of the complete line of gas controllers from Okolab. The Bold Line CO2 controller mixes air or N2 and CO2 and controls the
gas concentration with digital accuracy. Mix CO2 from 0 to 18 per cent concentration with an accuracy ± five per cent of the concentration. Set point resolution is 0.1 per cent. The instrument is easily operated via a touch screen interface, OKO-Control 2.0 software, or Nis Elements software. The controller can be attached to an O2 Unit-BL [1-20] to control both CO2 and O2 within the range 0 to 10 per cent and 0 to 18 per cent respectively. An optional smart box data logger and web server allows operation via any webenabled device. Sensor calibration is made with an external meter or calibration gas. Web: www.warneronline.com
Incubator Sheldon Manufacturing, Inc. introduces the SHEL LAB Model 2428H, the newest addition to its large capacity CO2 incubator line. This 27 cubic foot incubator features active humidity control up to 95 per cent. Compared to water pan humidity generation, the 2428H humidity system provides less evaporation of culture media and eliminates a potential source for contamination. The 2428H also has a heated glass door that minimizes condensation, another potential source for contamination. The triple-paned glass door allows easy viewing of samples without having to open the incubator door, so samples can thrive in the stable environment within the chamber. The incubator also features an antimicrobial copper drain and uses a gentle horizontal air flow heating system to obtain temperature uniformity and quick temperature recovery after door openings. Web: www.shellab.com.
Gas Chromatography Thermo Fisher
Scientific Inc. has added negative and positive ion chemical ionization (CI) capabilities to its new Thermo Scientific TSQ 8000 triple quadrupole GC-MS system. The addition of chemical ionization to the TSQ™ 8000 GC-MS/MS increases analytical options available to the user for addressing the diverse challenges of complex, trace level analysis. Chemical ionization can be used
for applications such as persistent organic pollutants (POPs), and methods that utilize halogenated derivatized reagents including estrogenic compounds or tetrahydrocannabinol (THC) in hair. In addition to the CI capability, Thermo Fisher also introduces direct insertion and direct exposure probe options, which further enhance the user’s ability to analyze samples in the TSQ 8000 mass spectrometer. These options are especially useful in combination with MS/ MS for applications such as the rapid structural characterization of chemical reaction products, which are important in chemical research and academic applications. Web: www.thermoscientific.com
Cell Imaging Andor Technology plc has launched its latest spinning disk confocal device, the Revolution WD for live cell confocal imaging. Taking a leap forward by offering four times the field of view, along with a new design for imaging at low and high power magnifications, the Revolution WD offers confocal images for larger samples as well as small. This new spinning disk confocal device offers a broad user base, appealing to new fields such as neuroscience, developmental biology, stem cell research, embryology and intra-vital imaging.
Sample handling Chemists gain a whole new perspective on sample handling with Vista Vial™ series chromatography vials from J.G. Finneran Associates. The patented, two-part design consists of a glass base and a polyethylene top that snaps together to form a standard 12x32mm auto sampler vial. The glass base provides a wide target for pipettes and is ideal for highly viscous and foaming samples. The polyethylene top is available with 8 to 425mm threaded, 9mm threaded or 11mm Snap Seal™ neck finishes offering chemists a choice of closure systems. Depending on the sample, disposal of vials is easier as the plastic can be incinerated intact without the need for closure removal. The Vista Vial™ 1.0mL volume glass base is constructed of Type 1 borosilicate glass in either clear or amber. The polyethylene top accommodates 8-425mm standard and 9mm R.A.M. screw thread closures and septa. Threaded closures and septa are available individually or preassembled. The Vista Vial™ with 11mm Snap Seal™ finish accepts J.G. Finneran Poly Crimp™ seals, Snap Top Caps™ and standard aluminum seals. Web: www.jgfinneran.com
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