11 minute read
New Products
Sequencing
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Illumina Inc. announces the launch of the TruSight® Tumor 15, a nextgeneration sequencing (NGS) panel designed to identify sequence variants in 15 genes commonly associated with marketed therapeutics. The panels are optimized for low input DNA from tissue preserved in formalin and paraffin, as well as for damaged and degraded formalin-fixed, paraffin embedded (FFPE) tumour samples. This enables low frequency somatic variant detection from limited nucleic acid inputs. When paired with the Illumina MiSeq® system, the panel delivers high-quality sequencing with key content and features needed for tumour analysis in translational research. The panel is intended for research use only and not for use in diagnostic procedures. Web: www.illumina.com/OncologyPanel
Spectrophotometers
The Thermo Scientific NanoDrop One and NanoDrop OneC UV-Vis microvolume spectrophotometers are designed to help life science researchers gain a more complete understanding of sample quality. These new spectrophotometers introduce the Thermo Scientific Acclaro Sample Intelligence technology, which enables researchers to identify sample contaminants and obtain corrected concentration results; receive instant feedback about sample quality with on-demand technical support and guided troubleshooting; and confidently measure samples with embedded sensor and digital image analysis. Moreover, the NanoDrop One instrument with its high-resolution touchscreen interface is simple to use, while its powerful auto-range path length technology facilitates accurate measurements for concentrated samples with no need for dilutions. The patented NanoDrop sample-retention system measures 1-2 µL of sample in seconds without the need for cuvettes, making it a cost- and time-effective solution for busy labs. Workflows can be streamlined on the NanoDrop One instrument with the Auto-Measure functionality, while modern connectivity allows results to be shared and archived via Wi-Fi, Ethernet or USB. The NanoDrop OneC instrument contains both pedestal and cuvette measuring positions, increasing dynamic range and assay flexibility. Web: www.thermoscientific.com/nanodrop
Mass Spectrometry
Bruker has released its new rapifleX MALDI-TOF/TOF mass spectrometer. With its redesigned, next-generation TOF/TOF ion optics, the rapifleX system now offers significantly higher speed, mass resolution and mass accuracy. The rapifleX integrates a novel, 10 kHz smartbeam™ 3D scanning laser design for breakthrough mass-spec imaging capabilities. It also offers dramatically improved spatial resolution, image contrast and quality, and a new ion source for superior robustness and throughput in pathology research. Tissue- and cell-type specific proteomics is a major advance for relevant biomarker discovery and identification, and requires MALDI tissue imaging studies which include the identification of detected biomarker candidates for validation and the subsequent development of targeted assays. The rapifleX MALDI-TOF/ TOF extends the MALDI Tissuetyper® imaging capabilities with rapid, spatially resolved MS/MS data. It supports the patented ImageID™ workflow for biomarker discovery in MALDI imaging cohort studies by obtaining matching identifications of tryptic peptides in bottom-up imaging studies. In addition to next-generation tissue imaging performance, the rapifleX MALDI-TOF/TOF system is well suited for detailed protein characterization in life science research and biopharmaceutical laboratories. The rapifleX™ MALDI-TOF/TOF system is for research use only. Web: www.bruker.com
Flow Meters
Sierra Instruments introduces its new InnovaMass 240i and 241i vortex mass flow meters. The new iSeries has been specifically designed for precise flow energy management in steam, compressed air, natural gas and water applications. Capable of measuring five process variables with one instrument, the InnovaMass flow meters feature a Raptor II operating system which runs apps like FloPro and Dial-A-Fluid, and a robust Smart Interface Portal (SIP) software providing end-users with flow measurement precision. Forms of energy in flow include heat energy (BTU or joules) in steam or water, the potential combustive energy in natural gas, and the potential expansion energy in compressed air, to name a few. Web: www.sierrainstruments.com
Software
Bio-Rad Laboratories, Inc. announces the release of its KnowItAll® spectroscopy software. One of the key enhancements of the new release is optimized corrections, capable of performing a computationally complex set of corrections on all query and reference spectra in a search to find the optimal match between the query and each reference spectrum. Multiple corrections are applied automatically to compensate for differences between spectra caused by the variability of different instruments and accessories and other factors including human error. This feature is critical as the inflexible mathematical algorithms traditionally employed in spectral searching cannot compensate for errors in spectra that are flawed. Moreover, while manual corrections can be made by expert spectroscopists, those less experienced in spectroscopy are often unaware of how to perform the necessary corrections on their sample spectrum to achieve the best result. KnowItAll 2015 also features two new software applications as well as the addition of spectra to its collection of Bio-Rad spectral databases that include: KnowItAll QC Expert™, KnowItAll Deformulation Expert™ and New Spectral Data. Web: www.bio-rad.com
new Products
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Well plates
The ExoComplete™ 96-Well Plate and ExoComplete™ Tube kits from Hitachi Chemical Diagnostics Inc. are an integrated, fast and easy system for exosome collection and mRNA purification. Both products are intended for research use only of molecular biology applications. Neither are intended for the diagnosis, prevention, or treatment of a disease, nor has either been validated for such use either alone or in combination with other products.The ExoComplete™ system isolates exosomal mRNA from biological samples such as plasma in a 96-well filter plate format, or largevolume samples such as urine in a single collection tube format. Exosomes and microvesicles (EMVs) in body fluids are captured with Hitachi Chemical’s Exosome Isolation Plate or Exosome Collection Tube with a proprietary filter material. The highly porous material allows fast filtration of biological samples without clogging, and reproducible isolation of EMVs without the use of conventional ultracentrifugation. After lysing the captured EMVs, mRNA can be isolated through hybridization with single strand oligo (dT) immobilized in the wells of the 96-well mRNA Capture Plate. A wide variety of downstream applications become available following isolation of mRNA, including mRNA quantification, single and double-stranded cDNA synthesis, RT-PCR, real time quantitative RT-PCR (qPCR), and next-generation RNA sequencing. Web: www.hcdiagnostics.com
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EchoTherm™
Torrey Pines Scientific announces the release of its EchoTherm™ Model IC50, peltier driven, chilling/heating dry bath with exact sample temperature control. The unit is supplied with a temperature probe to insert directly into the sample or into the sample block. The probe senses the sample temperature or sample block temperature directly and sends that information to the unit to drive and control the temperature exactly where set. There is also a sensor in the heater plate for allowing the user to set the plate temperature and use the probe to monitor the sample temperature. The Model IC50 displays and controls temperature to ±0.1°C. It can freeze, chill or heat samples from -10.0°C to 110.0°C in assay plates, centrifuge tubes of all sizes, vials, test tubes and other sample containers. It is particularly well suited to the molecular biology lab for doing hybridizations, sample prep for PCR, ligations, enzyme reactions, deactivations and much more. The Model IC50 is UL, CSA, and CE compliant. Web: www.torreypinesscientific.com
new Products
Assays
EMD Millipore announces the launch of two new assays kits, the MILLIPLEX® MAP Amyloid Beta and Tau Magnetic Bead Panel, and the MILLIPLEX® MAP Myokine Panel. Together these two kits enable the measurement of four key neurodegeneration biomarkers and 15 myokine proteins. The United States Food and Drug Administration (U.S. FDA) recently encouraged the exploration of amyloid beta 1-42, tau and phospho tau as biomarkers of Alzheimer’s disease and EMD Millipore’s MILLIPLEX® MAP Amyloid Beta and Tau Panel is the first commercially available multiplex kit that enables simultaneous measurement of amyloid beta 1-40, amyloid beta 1-42, tau and phospho tau. Secreted by skeletal muscle, myokines act as endocrine hormones that not only mediate skeletal muscle cell growth, but also affect immunity, neurological signaling and insulin response. The 15 myokines detected by EMD Millipore’s assay kit include brain-derived neurotrophic factor (BDNF) and irisin, whose once-debated function has been recently supported by proteomics studies. Web: www.emdmillipore.com
Mass Spectrometry
SCIEX introduces its next generation CESI-MS platform, the CESI 8000 Plus. This new platform further enables multiple experiments to be generated from as little as 5 microlitres (µL) of starting material. Combining this capability with the multi-segment injection methodology, one can process ten experiments in the time it would normally take to process one, with less than 1 µL consumed. This system is ideal for experiments where samples are limited, including microdialysates, needle biopsies, FFPE archives, rare circulating tumour cells or where samples are highly toxic. In addition, the innovation of neutral capillary surfaces further reduces system flow to less than 10 nanolitres/min (nL/min). The CESI 8000 Plus delivers high resolution separations, improves ionization efficiency and reduces ion suppression. Optional Laser-induced Fluorescence, Diode-Array and Selectable Wavelength UV detector modules are available, enabling off-line method development capability on this platform. The CESI 8000 Plus enables the separation of intact protein isoforms followed by mass spectrometry for researchers who need to understand the critical characteristics of biotherapeutics in development. CESI-MS provides a liquid-phase ion mobility separation, which now makes it possible to resolve closely related proteoforms, glycoforms, phosphorylated species and other post translational modifications (PTMs) on proteins. Web: www.sciex.com
Sample Management
Ziath has launched the DataPaq™ Cube Rack Reader, a new camerabased scanner for more effective sample management. The DataPaq™ Cube utilizes a dual camera set-up and unique cuboid design providing a flexible system for scanning 2D datamatrix coded tubes housed in all currently available rack formats.The reader works with all currently available rack types and can scan and decode a 96 tube rack in less than two seconds using standard computer hardware. Additional features include the easy saving of images for troubleshooting and diagnosis, and a fully integrated design within a single chassis. New templates can be easily created to match less common rack formats. All data is exportable into native Excel, CSV, text, XML and JSON formats to allow for advanced LIMS and robotic integrations. Advanced users can also send their results directly into a database. Web: www.ziath.com
Protein Purification Kits
Norgen Biotek has released new exosomal RNA kits for isolation of exosomes from plasma/serum, urine and cell culture media. The new kits are based on the use of Norgen’s proprietary resin to purify and concentrate the exosomes. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, RT-PCR, Northern blotting, RNase protection, primer extension, expression array assays and NGS. Additionally, the kits are simple, rapid and scalable and are designed to isolate all sizes of extracellular vesicle RNA, including microRNA. They do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/ chloroform or protease treatments. Web: www.norgenbiotek.com
Fermentation
Sartorius Stedim Biotech (SSB) announces the launch of its new ambr® 15 fermentation system, an automated micro bioreactor system designed to enhance microbial strain screening with advanced capabilities supporting fed-batch microbial cultures. It comprises 24 single-use stirred micro bioreactors (each with an 8-12mL working volume) integrated to a user-friendly, automated workstation. Delivering high oxygen transfer rates, rapid pH and feed additions, the systemis ideally suited for assessing the effects of different strains with microbial cultures. Additionally, it measures pH and DO every 12 seconds and there are pumped liquid lines for base and feed addition in each micro bioreactor, enabling tight pH control and a semi-continuous feed supply. Furthermore, the 10mL working volume allows multiple samplings throughout the fermentation run to assess culture growth and protein production, thus providing more detailed process understanding. Vessels are single use which eliminates cleaning and sterilization time. Web: www.sartorius.com
