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Nanobiotechnologies- applications, markets and companies Published on September 2011
Report Summary Nanotechnology is the creation and utilization of materials, devices, and systems through the control of matter on the nanometer-length scale (a nanometer is one billionth of a meter. Nanobiotechnology, an integration of physical sciences, molecular engineering, biology, chemistry and biotechnology holds considerable promise of advances in pharmaceuticals and healthcare. The report starts with an introduction to various techniques and materials that are relevant to nanobiotechnology. It includes some of the physical forms of energy such as nanolasers. Some of the technologies are scaling down such as microfluidics to nanofluidic biochips and others are constructions from bottom up. Application in life sciences research, particularly at the cell level sets the stage for role of nanobiotechnology in healthcare in subsequent chapters. Some of the earliest applications are in molecular diagnostics. Nanoparticles, particularly quantum dots, are playing important roles. In vitro diagnostics, does not have any of the safety concerns associated with the fate of nanoparticles introduced into the human body. Numerous nanodevices and nanosystems for sequencing single molecules of DNA are feasible. Various nanodiagnostics that have been reviewed will improve the sensitivity and extend the present limits of molecular diagnostics. An increasing use of nanobiotechnology by the pharmaceutical and biotechnology industries is anticipated. Nanotechnology will be applied at all stages of drug development - from formulations for optimal delivery to diagnostic applications in clinical trials. Many of the assays based on nanobiotechnology will enable high-throughput screening. Some of nanostructures such as fullerenes are themselves drug candidates as they allow precise grafting of active chemical groups in three-dimensional orientations. The most important pharmaceutical applications are in drug delivery. Apart from offering a solution to solubility problems, nanobiotechnology provides and intracellular delivery possibilities. Skin penetration is improved in transdermal drug delivery. A particularly effective application is as nonviral gene therapy vectors. Nanotechnology has the potential to provide controlled release devices with autonomous operation guided by the needs. Nanomedicine is now within the realm of reality starting with nanodiagnostics and drug delivery facilitated by nanobiotechnology. Miniature devices such as nanorobots could carry out integrated diagnosis and therapy by refined and minimally invasive procedures, nanosurgery, as an alternative to crude surgery. Nanotechnology will markedly improve the implants and tissue engineering approaches as well. There is some concern about the safety of nanoparticles introduced in the human body and released into the environment. Research is underway to address these issues. As yet there are no FDA directives to regulate nanobiotechnology but as products are ready to enter market, these are expected to be in place. Future nanobiotechnology markets are calculated on the basis of the background markets in the areas of application and the share of this market by new technologies and state of development at any given year in the future. This is based on a comprehensive and thorough review of the current status of nanobiotechnology, research work in progress and anticipated progress. There is definite indication of large growth of the market but it will be uneven and cannot be plotted as a steady growth curve. Marketing estimates are given according to areas of application, technologies and geographical distribution starting with 2010. The largest expansion is expected between the years 2015 and 2020. Profiles of 245 companies, out of over 500 involved in this area, are included in the last chapter along with their 183 collaborations.The report is supplemented with 41 Tables, 21 figures and 700 references to the literature.
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Table of Content TABLE OF CONTENTS 0. Executive Summary 19 1. Basics of Nanobiotechnology 21 Introduction 21 Classification of nanobiotechnologies 22 Top-down and bottom-up approaches 23 Landmarks in the evolution of nanobiotechnology 23 Relation of nanobiotechnology to healthcare 24 2. Technologies 27 Introduction 27 Micro- and nano-electromechanical systems 27 BioMEMS 27 Microarrays and nanoarrays 28 Dip Pen Nanolithography for nanoarrays 28 Protein nanoarrays 29 Microfluidics and nanofluidics 30 Nanotechnology on a chip 30 Microfluidic chips for nanoliter volumes 31 Nanogen's NanoChip 32 Use of nanotechnology in microfluidics 33 Construction of nanofluidic channels 33 Nanoscale flow visualization 34 Moving (levitation) of nanofluidic drops with physical forces 34 Electrochemical nanofluid injection 34 Nanofluidics on nanopatterned surfaces 35 Nano-interface in a microfluidic chip 35 Nanofluidic channels for study of DNA 35 Visualization and manipulation on nanoscale 36 4Pi microscope 36 Atomic force microscopy 36 Basic AFM operation 36 Advantages of AFM 36 Force sensing Integrated Readout and Active Tip 37 Cantilever technology 37 CytoViva速 Microscope System 39 Fluorescence Resonance Energy Transfer 39 Magnetic resonance force microscopy and nanoscale MRI 39 Multiple single-molecule fluorescence microscopy 40 Near-field scanning optical microscopy 40 Nano-sized light source for single cell endoscopy 40 Nanoparticle characterization by Halo' LM10 technology 41
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