1 CURRICULUM VITAE Harry Leon June, Ph.D. Professor of Psychiatry Director of Substance Abuse Professor of Pharmacology and Experimental Therapeutics University of Maryland School of Medicine May 10, 2010 OFFICE ADDRESS: University of Maryland School of Medicine Department of Psychiatry 110 S. Paca St, 4th Floor Baltimore, MD 21201 Phone: (443) 242-7125 Cell: (443) 691-3151 Fax: (410) 706-4002 Email: hjune@psych.umaryland.edu http://medschool.umaryland.edu/Psychiatry English (Native) Neuropsychopharmacology Laboratory: (410) 706-4019 EDUCATION: 1975-1979 B.S., Psychology/Biology, South Carolina State College, Orangeburg, SC 1982-1984 Practicum Student, Howard University, Child Development Ctr, Washington, DC 1982-1986 M.A., Clinical Psychology, University of the District of Columbia, Washington, DC 1983-1987 M.S., Physiological Psychology, Howard University, Washington, DC 1989-1990 Consortium Research Fellow in Database Development, Basic Research Laboratory, U.S. Army Research Institute for the Behavioral and Social Sciences, Alexandria, VA 1987-1990 Ph.D., Physiological Psychology/Animal Psychopharmacology, Minor: Personality Psychology, Howard University, Washington, DC POST GRADUATE EDUCATION AND TRAINING: 1989-1992 Postdoctoral Fellowship in Clinical Psychopharmacology, Behavioral Pharmacology Research Unit, Department of Psychiatry, Johns Hopkins University /Francis Scott Key Medical Center, Baltimore, MD Research Experience: 1984-1986 Research Associate, University of the District of Columbia, Agriculture Experimental Station, Mother-Infant Longitudinal Bonding Study. 1985 Research Assistant, University of the District of Columbia, Center for Applied Research and Urban Policy, Teenage Pregnancy Study. 1987-1989 Field Research Coordinator, University of the District of Columbia, Agriculture Experimental Station, District of Columbia Street Homeless Project.
2 1983-1989
1989-1992
Graduate Research Assistant, Howard University, brain stimulation reward, neurobiobehavioral effects of ethanol, conditioned taste aversion paradigms, feeding and drinking behavior, spontaneous activity measures. Postdoctoral Researcher, Behavioral Pharmacology Research Unit, Department of Psychiatry, Johns Hopkins University, responsibile for writing and implementation of research protocols investigating the psychopharmacodynamics of opioid withdrawal and dependence and the assessment of pharmacotherapeutic agents for the treatment of opioid dependence in human drug abusers.
Clinical Experience: 1980-1980 Weekend Relief Counselor, Montgomery County Association for Retarded Citizens, Kensington, MD, adult mental retardation. 1981-1982 Therapeutic Educational/Recreational Specialist, St. Elizabeth’s Hospital, Washington, DC, psychiatric and severe mental retardation 1982-1983 Psychological Assistant, St. Elizabeth’s Hospital, Washington, DC, psychological testing, behavioral and reality oriented therapy. 1982-1984 Practicum Student, Howard University, Child Development Center, Washington, DC, infant and child psychodiagnostics 1984-1985 Mental Health Worker, Alexandria Hospital, Alexandria, VA, psychiatric emergency 1982-1988 Residential Counselor, National Children’s Center, Washington, DC, child and adolescent mental retardation and emotional distrubance. 1983-1989 Grduate Researcher, University of the District of Columbia, evaluation of teacher styles in educationally handicapped students 1989-1992 Postdoctoral Researcher, Behavioral Pharmacology Research Unit, Department of Psychiatry, Johns Hopkins University, responsibile for writing and implementation of research protocols investigating the psychopharmacodynamics of opioid withdrawal and dependence and the assessment of pharmacotherapeutic agents for the treatment of opioid dependence in human drug abusers. EMPLOYMENT HISTORY: 1982-1985 Graduate Teaching Assistant, University of the District of Columbia, Department of Psychology 1983-1990 Graduate Teaching Assistant, Howard Unversity, Department of Psychology 1986-1988 Assistant Professor, University of the District of Columbia, Department of Psychology 1990-1992 Adjunct Lecturer, University of Maryland, Baltimore County, Department of Behavioral Medicine, Catonsville, Maryland 1993-1996 Adjunct Lecturer, Martin University, Department of Psychology Indianapolis, Indiana 1993-1998 Assistant Professor, Indiana University School of Medicine, Medical Neurobiology Program, Indianapolis, Indiana 1992-1998 Assistant Professor, Indiana University-Purdue University, Department of Psychology, Indianapolis, Indiana 1998-2003 Associate Professor, Indiana University School of Medicine, Medical Neurobiology Program, Indianapolis, Indiana 1998-2003 Associate Professor, Indiana University - Purdue University, Department of Psychology, Indianapolis, Indiana 2003-2005 Professor, Indiana University School of Medicine, Medical Neurobiology Program, Indianapolis, Indiana
3 2003-2005
Professor, Indiana University - Purdue University, Department of Psychology, Indianapolis, Indiana 2005-Present Professor, University of Maryland School of Medicine, Department of Psychiatry 2005-Present Director of Substance Abuse, University of Maryland School of Medicine, Department of Psychiatry 2005-Present Professor, University of Maryland School of Medicine, Department of Pharmacology and Experimental Therapeutics
PROFESSIONAL MEMBERSHIPS: 1989 Society for Neuroscience 1990 Research Society on Alcoholism 1992 Behavioral Pharmacology Society 1992 International Society of Biomedical Research on Alcoholism 1998-2003 Associate Member, American College of Neuropsychopharmacology 2003 Member, American College of Neuropsychopharmacology HONORS and AWARDS: 1987-1990 Minority Biomedical Research Support Fellowship [Graduate School] 1988 Patricia Harris Biomedical Research Award [Graduate School] 1991 NIDA Travel Award: Committee on Problems and Drug Dependence [Postdoctoral] 1991-1994 American College of Neuropsychopharmacology Minority Travel Award [Postdoctoral] 1994-1998 American College of Neuropsychopharmacology Mead Johnson Travel Award [Assistant Professor] 1997 Indiana University-Purdue University School of Science Research Award [Assistant Professor] 1998 Chancellor’s Bynum P. Mentorship Award, Indiana University-Purdue University [Associate Professor] 1998 Psychology Mentorship Award, Indiana University-PurdueUniversity [Associate Professor] INSTITUTIONAL SERVICE 1994-2003 Member, Minority Research Scholars Committee 1994-2003 Member, Psychobiology Curriculum Committee 1996 Member, Search Committee for Clinical Rehabilitative Psychology Faculty 1998 Member, Promotion and Tenure Committee, Department of Psychology 1994-2002 Member, Short-Term Training Program for Minority Students Interested In Biomedical Research Committee 1995-2003 Co-Coordinator Psychology Undergraduate Research Committee 2002-2005 NIAAA Training Grant Committee Member (IU School of Medicine) for Selection of Predoctoral and Postdoctoral Applicants 2003 Bepko Chancellor Scholarship Committee OTHER INSTITUTIONAL SERVICE: MASTERS THESES AND PH.D. DOCTORAL THESES COMMITTEE : 1992-94 Chair, Master's Thesis Committee in Psychobiology, Robert L. Hewitt: Tolerance Development to the Ethanol Suppressant Effects of the Benzodiazepine Inverse Agonist RO19-4603 (completed).
4 1992-95
Member, Ph.D. Dissertation Committee in Medical Neurobiology, Simon Kanter: Muscurinic Receptor Involvement in Alcohol Drinking and Behavioral Activity of Alcohol-Preferring (P) and-Nonpreferring (NP) Rats (completed).
1994-1997
Chair, Master's Thesis Committee in Psychobiology, Charity R. Cason: GABAergic Modulation of the Reinforcing Properties of Alcohol (completed).
1999-2002
Chair, Master's Thesis Committee in Psychobiology, Katrina L. Foster: A Systematic Evaluation of GABAA and Opioid Receptors of the Central Nucleus of the Amygdala in Regulating Ethanol and Sucrose-Maintained Responding (completed).
2000-2003
Chair, Master's Thesis Committee in Psychobiology, William JA Eiler II: The D1 Dopamine Receptor Regulates Alcohol-Motivated Behaviors in the Bed Nucleus of the Stria Terminalis in Alcohol-Preferring (P) Rats (completed).
2001-2004
Chair, Master's Thesis Committee in Psychobiology, Hardy, L., Responding for Brain Stimulation Reward (BSR) in the Bed Nucleus of the Stria Terminalis (BST) in High Alcohol Drinking (HAD) and Low Alcohol Drinking (LAD) Rat Lines (April 2004 completed).
1999-2004
Chair, Ph.D. Dissertation Committee in Psychobiology, Foster, K.L: The role of the GABAA Îą1 subtype in mediating the neurobehavioral effects of alcohol: studies using the Îą1 KO and WT mice (July 2004 completed).
2000-2004
Chair, Ph.D. Dissertation Committee in Psychobiology, Woods, J.E. II: The D1, but not the D2 dopamine receptors in the nucleus accumbens regulate the BSR threshold lowering effects of amphetamine in high alcohol drinking (HAD)-1 rats (November 2004 completed).
NATIONAL SERVICE: Member, Merit Review Subcommittee for Alcoholism, Drug Dependence, Anesthesiology and Analgesia (Veterans Administration) March 15, 2002-2003, Grant Reviewer Member, AA-1 Review Committee for National Institute of Alcoholism Abuse and Alcoholism (NIAAA) [R21 Grants] July 20, 2002-2006, Grant Reviewer Adjunct Reviewer, Neurotoxicology and Alcohol Study Section [NAL] Intergrative, ( NIAAA) [RO1 Grants] February 3, 2010, Grant Reviewer Ad Hoc JOURNAL REFEREE: Journal of Neuroscience, Neuropsychopharmacology, Synapse, Alcoholism:Clinical and Experimental Research, Pharmacology, Biochemistry and Behavior, Psychopharmacology, Behavioural Pharmacology, Brain Research-Molecular Section, PNAS
5 TEACHING SERVICE (see below): Behavioral Neuroscience [615] focuses on cellular, systems, and cognitive aspects of behavior. Behavioral Neuroscience is the initial core class that 1st year Ph.D. Psychobiology students are required to enroll in. Following the 615 class, graduate students are expected to enroll in Psychopharmacology [618]. Psychopharmacology is a class that focuses on the study of drugs and behavior in relation to neuropsychiatric disorders and drug dependence. A large focus of the class is on the molecular, neurobiological, and neuropharmacological aspects of neuropsychiatric disorders and drug dependence. The Drug Abuse [590] class represents an advanced class of the Ph.D. Psychobiology of Addictions curriculum. The class focuses on current methodological approaches and theoretical analyses that are used by today’s neuroscientists in understanding drug addiction. While the class was previously divided into 2 semesters, it is now a 1 semester class. Dr. June teaches the class by inviting “esteemed” neuroscientists from neighboring states, IU Medical Neurobiology Program, Eli Lilly and across the country to provide lectures. Drs. June and Goodlett also provide lectures. When notable speakers were brought in from Eli Lilly and across the country, Dr. June coordinated a university wide “Psychobiology Lecture Series”. The Psychobiology of Motivation and Reward [544] class is a specialty class wherein Dr. June teaches about the neurobiology of motivated behaviors. The class focuses on understanding the neuoanatomical substrates of motivated behavior. Equally important, the class provides the students with an understanding of past and present theories of reward and reinforcement. Due to the interdisciplinary nature of many of these classes and their clinical relevance, several are appealing to Clinical Rehabilitation, Medical Neurobiology, and some social work Ph.D. students. Medical school M.D./Ph.D. students are also often enrolled in several of the classes. Finally, Graduate Seminar [591] is a literature topic class wherein both graduate students in Psychobiology and Medical Neurobiology convene to discuss current literature in the drug abuse field. It is also important to note that several of these classes are cross referenced within the Medical Neurobiology Program curriculum in the Dept. of Psychiatry, IU School of Medicine. B320, Behavioral Neuroscience [formerly known as Physiological Psychology] is an upper level undergraduate psychology class designed to teach students about the physiological processes regulating psychological behavior with emphasis placed on both the central and peripheral nervous systems. As with the graduate class, this class focuses on cellular, systems, and cognitive aspects of behavior. The teaching format comprises a lecture-seminar format and students are routinely asked to verbally contribute to the class during lectures. Exams are 70% essay, and a topic paper is written and presented by the students to their peers. The topic paper is 10-15% of the student's total grade. Brain Mechanisms [B452] SP2004 is an advanced undergraduate class which deals with cellular diversity within the CNS, and the role of various second and third messenger systems in regulating motivated behavior as well as learning and memory. Finally, the 105 class as noted below in an introductory class that Dr. June teaches every 3 years.
6 Classes Taught at IUPUI Psychology Department _______________________________________________________________________________ Name of course
Semester
G/U
Course #
N
Time Spent in Classroom during a 16 week semester ________________________________________________________________________________ Psychopharmacol Physio Psy Physio Psy Intro to Psych Physio Psy Physio Psy Psycho Motivation Physio Psy Intro to Psych Drug Abuse Behav Neurosci Physio Psy Behav Neurosci Drug Abuse Brain Mechanisms of Behavior Graduate Seminar Psycho Motivation
Fall 93 Fall98 SP98 Sum98 Sum98 Fall99 SP99 Fall2000 Fall 2000 SP2000 Fall 2001 SP2001 SP2002 Fall2003 SP2004
G U U U U U G U U G G U U G U
B618 B320 B320 B105 B320 B320 B544 B320 B105 B590 B615 B320 B320 B590 B452
11 12 19 19 5 18 6 18 52 6 14 22 22 8 4
2.5 hr 2.5 hr 2.5 hr 4.5 hr 4.5 hr 2.5 hr 2.5 hr 2.5 hr 2.5 hr 2.5 hr 2.5 hr 2.5 hr 2.5 hr 2.5 hr 2.5 hr
SP2004 F2004
G G
B591 B544
9 5
2.0 hr 5 hr
_______________________________________________________________________________ U- Represents an Undergraduate Class G- Represents a Graduate Class N- # of Students Report on School Review of Mentoring: In the mentoring reports given below students are asked to complete a "Faculty Mentoring Report" prior to graduating from IUPUI. Students can identifty faculty members at three levels. In the reports summed over the past 5 years (1997-2001) Dr June was selected as Mentor with the following frequency: Total in Associate Professor Rank [1997 to 2002] Level 1--faculty who have had a remarkable and positive influence on them but are not in their top three list (9 times) Level 2--the top three faculty who have a remarkable and positive influence on them (12 times) Level 3--the faculty member who have influenced the whole course of their lives and whose effects were invaluable (6 times) Total in Full Professor Rank [2002 Spring to present] Level 1--faculty who have had a remarkable and positive influence on them but are not in their top three list (6 times) Level 2--the top three faculty who have a remarkable and positive influence on them (3 times) Level 3--the faculty member who have influenced the whole course of their lives and whose effects were invaluable (2 times)
7 Awards Related to Mentoring 1998, Chancellor’s Bynum P. Mentorship Award, Indiana University-PurdueUniversity. This award is presented to a full-time faculty member in the Purdue, IU, or IU Medical School by the chancellor of IUPUI for outstanding performance in mentoring of graduate, undergraduate, or students in any of the professional schools at the Indianapolis campus 1998, Psychology Mentorship Award, Indiana University-PurdueUniversity. This award is presented to a full-time psychology faculty member in the Purdue School of Science at the Indianapolis campus GRANT SUPPORT: Extramural Prior and Present Research Support A. “Benzodiazepine Actions on Alcohol Reinforcement” Principal Investigator: Harry L. June Agency: National Institute of Alcohol Abuse and Alcoholism Type: R29 (AA10406-01A2) Period April 1, 1997 – March 30, 2002 $350,000 direct cost, $520,000 total cost The overall goal of this project is to systemically evaluate benzodiazepine receptor ligands for their capacity to reduce alcohol-motivated reresponding. B. “GABAa Receptor Mechanisms in Alcohol Reinforcement” Principal Investigator: Harry L. June Agency: National Institute of Alcohol Abuse and Alcoholism Type: R21 (AA11555-01A1) Period Sept 1, 1999 – May 30, 2002 $376, 501 direct cost, $564,000 total cost The overall goal of the project is to systematically identify CNS substrates of GABAergic transmission in the extended amygdala that regulate EtOH-motivated behaviors. C. “GABAa Receptor Subunits Alcohol Reinforcement” Principal Investigator: Harry L. June Agency: National Institute of Alcohol Abuse and Alcoholism Type: R01 (AA12407) Period April 1, 2002 – March 30, 2007 $875,000 direct cost, $1,158,249 total cost The overall goal of the project is to evaluate the GABA Alpha 1 and 5 receptor subunits in EtOH reinforcement employing GABA Alpha 1 and 5 receptor ligands. D. “The Alpha-1 GABAA Receptor Regulates Alcohol-Drinking Behaviors” Principal Investigator: Harry L. June Agency: National Institute of Alcohol Abuse and Alcoholism Type: R21 (AA017461) Period April 1, 2008 – April 30, 2010 $385,000 direct cost, $568, 465 total cost The overall goal of this project is to employ the RNAi procedure to silence the GABA Alpha-1 receptor gene to investigate its effects on alcohol drinking.
8 E. “Efficacy of Novel Triple Uptake Inhibitors in Treating Alcoholism and Depression” Principal Investigator: Harry L. June Agency: National Institute of Alcohol Abuse and Alcoholism Type: R01 (AA17461-2) Period December 30, 2008 – Nov 30, 2013 $1,250,000 direct cost, $1,875,000 total cost The overall goal of this project is to systemically evaluate novel triple uptake inhibitors in treating alcoholism and depression using alcoholic rats. [Active] F. “Anxiety and Alcoholism: Novel Benzodiazepine Treatments” Principal Investigator: Harry L. June Agency: National Institute of Alcohol Abuse and Alcoholism Type: R01 (AA017963-01A1) Period September 30, 2009 – August 31, 2011 $541,000 direct cost, $791,000 total cost The initial objective of this proposal is to synthesize novel α1 GABAA subtype-preferring ligands for further evaluation of clinical efficacy in treating both excessive alcohol drinking (e.g., binge) and the negative affective states (e.g., anxiety, anhedonia) associated with abstinence. The secondary objective is to employ molecular biology procedures [e.g., HEK cell, siRNA, immunocytochemistry] to determine the "precise" GABAA receptor subtype in which the novel α1 ligands inhibit excessive alcohol drinking. [Active] Pending Grants A. GABAA related neuroinflammation regulates binge drinking Principal Investigators: Harry L. June and Laure Aurelian Agency: National Institute of Alcohol Abuse and Alcoholism Type: R01 (AA020080-01) Period August 31, 2010 – July 31, 2015 $1,250,000 direct cost The overall goal of this project is to examine the interaction of GABAA receptors and neuroimmune factors in regulating binge drinking. [Pending review in June 2010] B. “GABAA regulated neuroinflammation in alcoholism therapy” Principal Investigator: Harry L. June and Laure Aurelian Agency: National Institute of Alcohol Abuse and Alcoholism Type: RC4 (AA020128-01) Period September 31, 2010 – August 31, 2013 $1,500,000 direct cost The overall goal of this is to examine the interation of GABAA receptors and neuroimmune factors in regulating binge and relapse drinking [Pending review in July 2010]
Intramural A. IUPUI Summer Research Fellowship Grant "Neurobehavioral Determinants of Ethanol Reward" (Summer 5/93-8/93) $6,000, Faculty Development Office (Completed) B. IUPUI Research Aid Grant "Neurobehavioral Determinants of Ethanol Reward" 5/93-9/93, $3,000, Faculty Development Office (Completed)
9
Student Grant/Fellowship Support 1. †IUPUI Mentorship Grant for Terri Greene "The Novel Benzodiazepine Inverse Agonist RO19-4603 decreases EtOH intake in alcohol preferring (P) rats. 9/94-5/95, $1,500 (Completed) 2. IUPUI Student Undergraduate Research (SUR) Grant for Scott Duemler, "Interactions of RO19-4603 and Ethanol on Tests of Anxiety, Exploration, and Locomotion" 1/945/94, $1,500, Honors Research Program (Completed) 3. †IUPUI Mentorship Grant for Samantha Dejaravu "Effects of the Benzodiazepine Antagonist, CSG 8216 on Ethanol Intake and the Locomotor Depressant Effects of Ethanol" 5/94-8/94, $1,500, Student Research Opportunity Program for Minority Students (SROP), Honors Research Program (Completed) 4. †IUPUI Mentorship Grant for Connease Warren-Reese "Evaluation of novel benzodiazepine receptor ligands in high (HAD) and low (LAD) alcohol-preferring rats" 5/94-8/94, $1,500, Student Research Opportunity Program for Minority Students (SROP), Honors Research Program (Completed) 5. †Short Term Summer Research Fellowship in Biomedical Research for Terri L. Greene, "Differential Sensitivity of Benzodiazepine Inverse Agonists in Alcohol-Preferring (P) and Non-preferring (NP) Rats" 6/94-8/94, $1,600, IUSM, NIH (Completed) 6. IUPUI Student Undergraduate Research Award (SUR) IUPUI for John Williams, "Interactions of RO19-4603 and Ethanol on Tests of Anxiety, Exploration, and Locomotion" 9/94-5/95, $1,500, Honors Research Program (Completed) 7. †IUPUI Mentorship Grant for Lucas Torres , "Evaluation of Novel Benzodiazepine Partial Agonists in Rats With and Without a Predisposition to Alcoholism 9/94-5/96, $1,500 (Completed) 8. †Short Term Summer Research Fellowship in Biomedical Research for Lucas Torres, "Evaluation of the anxiolytic properties of novel benzodiazepine receptor ligands in rats with a genetic predisposition to alcoholism " 5/95-8/95, $1,600, IUSM, NIH (Completed) 9. IUPUI Student Undergraduate Research (SUR) Grant for Mary Becky Eggers "Effects of the Benzodiazepine Antagonist Ethanol Responding " 9/95-5/96, $1,200, Honors Research Program (Completed) 10. IUPUI Mentorship Grant for Dana Zuccarelli, " Effects of Novel Benzodiazepine Antagonist on Ethanol Responding" 12/95-5/96, $1,000, Faculty Development Office (Completed) 11. †Short Term Summer Research Fellowship in Biomedical Research for Connease Warren- Reese "Characterization of nalmefene, the 6- methylene derivative of naltrexone to antagonize ethanol responding in alcohol-preferring rats" 5/96-8/96, $1,600, IUSM, NIH (Completed)
10 12. †Short Term Summer Research Fellowship in Biomedical Research for LaFranze Durr, "Benzodiazepine with different intrinsic efficacies antagonize ethanol responding in alcohol-preferring rats" 5/96-8/96, $1,600, IUSM, NIH (Completed) 13. †IUPUI Mentorship Grant for Anila Ricks-Cord, "Characterization of nalmefene, the 6 methylene derivative of naltrexone to antagonize ethanol responding in alcoholpreferring rats" 5/96-8/96, $1,500, Student Research Opportunity Program for Minority Students (SROP), Honors Research Program (Completed) 14. IUPUI Mentorship Grant for Collette Grey, "Novel benzodiazepine antagonists reduce responding for reinforcement by ethanol. in alcohol-preferring rats" " 1/98-8/98, $1,000, Faculty Development Office (Completed) 15. IUPUI Student Undergraduate Research (SUR) Grant for Pete McKay, "RY 023 reduces EtOH-maintained responding following injection into the CA1 and CA3 hippocampal fields." 12/98-5/99, $1,500, Honors Research Program (Completed) 16. †Short Term Research Fellowship in Biomedical Research for Charity Cason, "R0-194603 reduces EtOH-maintained responding following injection into the Nucleus Accumbens." 1/99-5/99, $3,000, IUSM, NIH (Completed) 17. IUPUI Undergraduate Research Opportunity Program (UROP) Grant for Pete McKay, "RY 023 reduces EtOH-maintained responding following injection into the CA1 and CA3 hippocampal fields." 1/99-5/99, $1,500 (Completed) 18. †Short Term Research Fellowship in Biomedical Research for Katrina Foster, "RY 023 reduces EtOH-maintained responding following injection into the CA1 and CA3 hippocampal fields." 1/99-5/99, $3,000, IUSM, NIH (Completed) 19. IUPUI Undergraduate Research Opportunity Program (UROP) for Michelle Carroll, "Novel benzodiazepine antagonists infused into the ventral pallidal area reduce responding for reinforcement by ethanol. in alcohol-preferring rats" " 12/99-5/00, $1,600 (Completed) 20. IUPUI Undergraduate Research Opportunity Program (UROP) for William Eiler, "Role of the bed nucleus of the stria terminalis in brain stimulation reward in HAD and LAD rats" 1/00-5/00, $1,600 (Completed) 21. †Short Term Research Fellowship in Biomedical Research for Dana Milbourn. GABA and Opioids Within the Central Amygdala (CeA) Regulate Ethanol-Maintained Responding. 5/00-8/00, IUSM, NIH (Completed) 22. †Short Term Research Fellowship in Biomedical Research for Lorie La Duff. Responding for Brain Stimulation within the Medial Forebrain Bundle Before and After Amphetamine Pretreatment in Alcohol-preferring (P) and Nonalcohol-preferring (NP) Rat Lines. 5/00-8/00, IUSM, NIH (Completed) 23. †Short Term Research Fellowship in Biomedical Research for Chaz Mailey. The D1 Dopamine Receptor Regulate Alcohol-Motivated Behaviors in the Bed Nucleus of the Stria Terminalis (BNST) in Alcohol-preferring (P) rats. 5/01-8/01, IUSM, NIH (Completed)
11 24. †Short Term Research Fellowship in Biomedical Research for Lathen Hardy. Responding for Brain Stimulation Reward (BSR) in the the Bed Nucleus of the Stria Terminalis (BST) in High Alcohol Drinking (HAD) and Low Alcohol Drinking (LAD) Rat Lines 5/01-8/01, IUSM, NIH (Completed) 25. †Short Term Research Fellowship in Biomedical Research for Chaz Mailey. Novel Alpha-1 Receptor Ligands Regulate Alcohol-Motivated Behaviors: Comparison with Naltrexone 5/02-8/02, IUSM, NIH (Completed) 26. †Short Term Research Fellowship in Biomedical Research for Lathen Hardy. Responding for Brain Stimulation Reward (BSR) in the the Bed Nucleus of the Stria Terminalis (BST) in Alcohol-preferring (P) and Nonalcohol-preferring (NP) Rat Lines. 5/02-8/02, IUSM, NIH (Completed) 27. †Short Term Research Fellowship in Biomedical Research for Sandra Nobel. GABA/DA Interaction in the Bed Nucleus of the Stria Terminalis (BST) Regulate Alcohol-Motivated Behaviors. 5/02-8/02, IUSM, NIH (Completed) † - Represents student with minority status. PATENTS APPLICATION FILED: [JULY 9th, 2009] “Methods for Reducing Alcohol Craving in Chronic Alcoholics”. Patent proposes to use Alpha 1 selective benzodiazepine antagonists to reduce alcohol drinking via direct blockade of the reinforcing effects. An additional, a novel feature of the invention is that the Alpha 1 agents also reduce the anxiety that alcoholics experience upon the withdrawal from alcohol. PUBLICATIONS: Peer Reviewed Journal Articles: 1. June, H.L., & Lewis, M.J. (1989) Ro15-4513 attenuates and enhances open-field activity in rats. Alcohol 6, 245-248. 2. June, H.L., Johnson, L.T., & Lewis, M.J. (1989) Ro15-4513 attenuates depression of open-field horizontal activity. Alcohol 6, 335-337. 3. June, H.L., Hicks, L.H., Lewis, M.J., & Ross, S. (1990) Denatonium saccharide as an aversive stimulus in a conditioned taste aversion pardigm. Perceptual and Motor Skills 71, 1299-1307. 4. Lewis, M.J., & June, H.L. (1990) Neurobehavioral effects of ethanol on brain stimulation reward and spontaneous measures of activity, Alcohol 7, 213-219. 5. June, H.L., Lummis, G., Colker, R.E., Moore, T.O., & Lewis, M. J. (1991) Ro15-4513 decreases ethanol consumption in deprived rats. Alcoholism: Clinical and Experimental Research 15, 406-411. 6. Stitzer, M.L., Wright, C., Bigelow, G.E., June, H.L., & Felch, L.J. (1992) Time course of naloxone-precipitated withdrawal after acute methadone exposure in humans. Drug & Alcohol Dependence 29, 39-46.
12 7. June, H.L., June, P.L., Domangue, K.R., Lummis, G. L., & Lewis, M.J. (1992) Failure of Ro15-4513 to alter ethanol-induced CTA in rats. Pharmacology, Biochemistry and Behavior 41, 455-460. 8. June, H.L., Colker, R.E., Domangue, K.R., Hicks, L.H., June, P.L., & Lewis, M.J. (1992) Ethanol self-administration in deprived rats: effects of Ro15-4513 alone, and in combination with flumazenil (Ro15-1788). Alcoholism: Clinical and Experimental Research 16, 11-16. 9. Moore, T.O., June, H. L., & Lewis, M.J. (1994) Ethanol induced stimulation and depression on measures of locomotor activity: effects of basal activity levels in rats. Alcohol 10, 537-540. 10. Lewis, M. J., & June, H. L. (1994) Synergistic effects of ethanol and cocaine on brain stimulation reward. Journal of Experimental Analysis of Behavior 61, 223-229. 11. June, H. L., Murphy, J.M., Mellor-Burke, J.J., Lumeng, L. & Li, T-K. (1994) The inverse benzodiazepine agonist RO19-4603 exerts prolonged and selective suppression of ethanol intake in alcohol-preferring (P) rats. Psychopharmacology 115, 325-331. 12. June, H. L., Hughes, R. W., Spurlock, K. R., Domangue, K. R., & Lewis, M. L. (1994) Ethanol self-administration in freely-feeding rats: effects of Ro15-4513 alone, and in combination with flumazenil (Ro15-1788). Psychopharmacology 115, 332-339. 13. June, H. L., & Lewis, M. J. (1994) Interactions of Ro15-4513 and Ro15-1788 (Flumazenil) on measures of exploration and locomotion in rats. Psychopharmacology 116, 309-316. 14. June, H.L., Stitzer, M.L. & Cone, E. (1995). Acute opioid dependence in humans: relation to plasma morphine concentration. Clinical Pharmacology and Therapeutics 57, 270-280. 15. Walsh, S.L., June, H.L., Schuh, K.J., Preston, K.L., Bigelow, G.E., & Stitzer, M.L. (1995) Effects of buprenorphine and methadone in methadone-maintained subjects. Psychopharmacology 119, 268-277. 16. June, H.L., Lin, M., Greene, T.L., Lewis, M.J., & Murphy, J.M. (1995) Effects of negative modulators of GABAergic efficacy on ethanol intake: correlation of biochemical changes with pharmacological effect using behavioral paradigm. Journal of Experimental and Clinical Psychopharmacology 3, 252-260. 17. June, H.L., Duemler, S., Greene, T.L., Williams, J., Lin, M., Chen, S.H.A., Lewis, M.J. & Murphy, J.M. (1995) Effects of RO19-4603 on the maintenance of tolerance to a single dose of ethanol. Journal of Pharmacology and Experimental Therapeutics 247, 11051112. 18. June, H.L., Murphy, J.M., Hewitt, R.L., Greene, T.L., Lin, M., Mellor-Burke, J., Lumeng, L., & Li, T.-K. (1996) Benzodiazepine receptor ligands with different intrinsic efficacies alter ethanol intake in alcohol-nonpreferring (NP) rats. Neuropsychopharmacology 14, 55-66
13 19. Greenwald, M., June, H.L., Marco, A., & Stitzer, M.L. (1996) Clinical pharmacology of fentanyl in drug abusers. Journal of Pharmacology and Experimental Therapeutics 277, 1228-1235. 20. June, H.L., Greene, T.L., Murphy, J.M., Lin, M., Williams, J.A, Cason, C.R., MellorBurke, J.J., Duemler, S. Torres, L., Lumeng, L. & Li, T-.K. (1996) Effects of the benzodiazepine inverse agonist RO19-4603 alone, and in combination with the benzodiazepine receptor antagonists, flumazenil, ZK 93426, and CGS 8216 on ethanol intake in alcohol-preferring (P) rats. Brain Research 734, 19-34. 21. June, H.L., Torres, L., Cason, C.R., Hwang, B.H., Braun, M.R., Murphy, J.M. (1998) The novel benzodiazepine inverse agonist RO19-4603 antagonizes ethanol motivated behaviors: neuropharmacological studies. Brain Research 784, 256-275. 22. June, H.L., Dejaravu, S.L., Williams, J., Cason, C.R., Eggers, M.W., Greene, T.L., Leviege, T., Torres, L. Braun, M.R., Murphy, J.M. (1998) GABAergic modulation of the behavioral actions of ethanol in alcohol-preferring (P) and nonpreferring (NP) rats. European Journal of Pharmacology 342, 139-151. 23. June, H.L., Zucarelli, D., Craig, K.S., DeLong, Jana, Cason, C.R., Torres, L., & Murphy, J.M. (1998) High affinity benzodiazepine antagonists reduce responding maintained by EtOH presentation in ethanol-preferring (P) rats. Journal of Pharmacology and Experimental Therapeutics 284, 1006-1014. 24. June, H. L., Cason, C. R., Cheatham, G., Ruiyan L. Gan T., & Cook, J.M. (1998) GABAA-benzodiazepine receptors in the striatum are involved in the sedation produced by a moderate, but not an intoxicating ethanol dose in outbred Wistar rats. Brain Research 794, 103-118. 25. June, H.L., Eggers, M.W., Warren-Reese, C., Ricks, A., & Cason, C.R. (1998) The effects of the novel benzodiazepine receptor inverse agonist Ru 34000 on ethanolmaintained responding. European Journal of Pharmacology 350, 151-158. 26. June, H. L., Cason, C. R., Chen, S.H.A., & Lewis, M. J. (1998) Dose and timedependent effects of buprenorphine on ethanol self-administration in rat. Psychopharmacology 140, 29-37. 27. June, H.L., Grey, C., Warren-Reese, C., Lawrence, A. Thomas, A., Cummings, R., Williams, L., McCane, S.L., Durr, L.F., Mason, D. (1998) The opioid receptor antagonist nalmefene reduces alcohol-motivated behaviors: preclinical studies in alcohol preferring (P) and outbred Wistar rats. Alcoholism: Clinical and Experimental Research 22, 2174-2185. 28. June, H.L., McCane, S., Zink, R.W., Portoghese, P., Li, T.-K. & Froehlich, J.C. (1999) The δ 2 opioid receptor antagonist naltriben reduces alcohol-motivated behaviors. Psychopharmacology 147, 81-89. 29. June, H.L., Harvey, S.C., Foster, K.L., McKay, P.F., Cummings, R., Garcia M., Mason, D., Grey, C., McCane, S.L Williams, L., Durr, L.F., Johnson, T.B., Xiaohui, He, Rock, S., & Cook, J.M (2001) GABAA-receptors containing ι5 subunits in the CA1 and CA3 hippocampal fields regulate ethanol-motivated behaviors: An extended ethanol reward circuitry. Journal of Neuroscience 21, 2166-2177.
14 30. Harvey S.C., Foster K.L., McKay P.F., Carroll Mm, Seyoum R., Woods J.E. II, Grey C., McCane S.,Cummings R., Mason D., Jones C.M., Ma C., Cook J.M., & June H.L. (2002) The GABAA receptor Îą1 subtype in the ventral pallidum regulates EtOH-seeking behaviors. Journal of Neuroscience 22, 3766-3775. 31. Eiler II, W.J.A., Seyoum, R., Foster, K.L., Mailey, C, and & June, H.L. (2003) The D1 dopamine receptor regulates alcohol-motivated behaviors in the bed nucleus of the stria terminalis in Alcohol-Preferring (P) rats. Synapse 48, 45-56. 32. Woods, J.E. II, McKay, P.F. Seyoum, R., Chen, A., Masters, J., La Duff, L., Lewis, M.J., & June, H.L. (2003) Differential Responding for brain-stimulation reward and sucrose in High Alcohol Drinking (HAD) and Low Alcohol Drinking (LAD) Rats. Alcoholism: Clinical and Experimental Research 27, 926-936. 33. June, H.L., Foster, K.L., McKay, P.F., Carroll, M.R., Seyoum, R., Woods, J.E., Harvey, S.C., Eiler, W.J.A. II, Grey, C., McCane, S., Garcia, M., Jones, C.M., Mason, D., Cummings, R., Yin, W., Cook, J.M., & Skolnick, P. (2003) The reinforcing properties of alcohol are mediated by GABA(A1) receptors in the ventral pallidum. Neuropsychopharmacology 28, 2124-2137. 34. June, H.L, Cummings, R., Eiler, W.J.A. II, Foster, K.L., Garcia, M., Mason, D., McKay, P.F., Hawkins, S., & Mason, M. (2004) Central opioid receptors differentially regulate the nalmefene-induced suppression of ethanol- and saccharin-reinforced behaviors in alcohol-preferring (P) rats. Neuropsychopharmacology 29, 285-299. 35. McKay P.F., Foster K.L., Mason D., Cummings R., Garcia M., Williams. L., Eiler, W.J.A. II, Woods J.E., Harvey S.C., Garcia M., Grey C., Grey C., Mc Cane S., Xiaohui H., Cook, J.M., & June H.L. (2004) A high affinity ligand for GABAA-receptor containing alpha5 subunit antagonizes ethanol's neurobehavioral effects in Long-Evans rats. Psychopharmacology 172, 455-462. 36. Foster K.L., McKay P.F., Seyoum R., Milbourne D., Yin W., Cook J.M., June H.L. (2004) GABA(A) and opioid receptors of the central nucleus of the amygdala selectively regulate ethanol-reinforced behaviors. Neuropsychopharmacology 29, 269-284. 37. Eiler II, W.J.A., Woods II, J.E., Masters, J., McKay, P.F., Hardy III, L., Goergen, J.J., Mensah-Zoe, B., Cook, J.B., Johnson, N.J., & June, H.L. (2005) Brain stimulation reward performance and sucrose maintained behaviors in alcohol preferring (P) and non-preferring (NP) rats. Alcoholism: Clinical and Experimental Research 4, 571-83 38. Cook J.B., Foster K.L., McKay P.F., Harvey S.C., Carroll Mm, Seyoum R., Woods J.E. II, Grey C., McCane S.,Cummings R., Mason D., Jones C.M., Ma C., Cook J.M., & June H.L. (2005) Further studies of the GABAA receptor Îą5 subtype in regulating EtOH-seeking behaviors. Alcoholism: Clinical and Experimental Research 9, 1390-401. 39. Eiler II, W.J.A., Masters, J., McKay, P.F., Hardy III, L., Goergen, J.J., Mensah-Zoe, B., Seyoum, R., Cook, J.B., Johnson, N.J., Neal-Beliveau, B., & June, H.L. (2006) Amphetamine lowers brain stimulation reward (BSR) threshold in alcohol preferring (P) and non-preferring (NP) rats: regulation by D1 and D2 receptors in the nucleus accumbens. Experimental and Clinical Psychopharmacology 14, 361-76.
15 40. Chester, J.A., Rausch, E.J., June, H.L., & Froehlich J.C. (2006) Decreased reward during acute alcohol withdrawal in rats selectively bred for low alcohol drinking. Alcohol. 38, 165-72. 41. Eiler II, W.J.A., & June, H.L. (2007) Blockade of GABA(A) receptors within the extended amygdala attenuates D(2) regulation of alcohol-motivated behaviors in the ventral tegmental area of alcohol-preferring (P) rats. Neuropharmacology. 52, 1570-9. 42. June, H.L. Sr, Foster, K.L., Eiler II, W.J.A., Goergen, J., Cook, J.B., Johnson, N., Mensah-Zoe, B., Simmons, J.O., June, H.L. Jr, Yin, W., Cook, J.M., & Homanics, G.E. (2007) Dopamine and benzodiazepine-dependent mechanisms regulate the EtOHenhanced locomotor stimulation in the GABAA alpha1 subunit null mutant mice. Neuropsychopharmacology. 32, 137-52. 43. Eiler II W.J.A., Hardy, L., Woods, J., Seyoum, R., & June, H.L. (2007) Responding for Brain Stimulation Reward (BSR) in the Bed Nucleus of the Stria Terminalis (BST) in Alcohol Preferring (P) Rats Following EtOH and Amphetamine Pretreatments. Synapse. 61, 912-24. 44. June H.L., Tzeng Yang A.R., Bryant J.L., Jones O., Royal W 3rd. (2009) Vitamin A deficiency and behavioral and motor deficits in the human immunodeficiency virus type 1 transgenic rat. Journal of Neurovirology. 15(5-6):380-9. 45. Yang A.R., Yi H.S., Mamczarz J., June H.L. Jr., Hwang B.H., June H.L. Sr. (2009) Deficits in substance P mRNA levels in the CeA are inversely associated with alcoholmotivated responding. Synapse. 63, 972-81. 46. Wiltgen B.J., Godsil B.P., Peng Z., Saab F., June H.L., Linn M.L., Cook J.M., Houser C.R., O'Dell T.J., Homanics G.E., Fanselow M.S. (2009) The alpha1 subunit of the GABA(A) receptor modulates fear learning and plasticity in the lateral amygdala. Frontiers in Behavioral Neuroscience. 3:37. 47. Yang, A.R.S.T., Mamczarz, J., Yi, H.S., Mallick, N., Krieter, P.A., Skolnick, P., Basile, A.S., June, H.L. (in press) Evaluation of DOV 102,677, the Triple Monoamine Uptake Inhibitor on Alcohol-Motivated Responding and a Rat Model of Antidepressant Activity in Alcohol-Preferring (P) Rats. Alcoholism: Clinical and Experimental Research. 48. Eiler II, W.J.A., Cook, J.B., Johnson, N., Mensah-Zoe, B., June, H.L. Jr, Homanics G.E., June H.L. Sr. (Submitted) Differential responses elicited by EtOH and BDZ treatments alone, and the combination of the two on motor-performance measures in GABA alpha1 subunit null mutant and wild-type mice. Alcoholism: Clinical and Experimental Research 49. Lui, J., Yang, A.R.S.T., Kelly, T., Puche, A.C., Elnabawi, A., Merchenthaler, I., Esoga, C., Sieghart, W., June H.L., Aurelian, L. (Submitted) GABAA Îą2-regulated innate immunity and neuroinflammation in the central amygdala: key players in binge alcohol drinking? Journal of Neuroscience
16 Book Chapters: June, H.L., (2002) “Alcohol Initiation Procedures in Rats: Methods Used in Evaluating Potential Pharmacotherapeutic Agents,” In Current Protocols in Neuroscience (Edited by Crawley, J., Gerfen, C., McKay, R., Rogawski, M., Sibley, D., & Skolnick, P.). John Wiley and Sons, NY, NY, 9:1-23 June, H.L., & Eiler II, W.J.A. (2007). “Dopaminergic and Gabaergic Regulation of AlcoholMotivated Behaviors: Novel Neuroanatomical Substrates,” In Handbook of Contemporary Neuropharmacology ([Editor-in-Chief: Sibley, D.] [Associate Editors: Hanin, I., Kuhar, M., & Skolnick, P.]) John Wiley and Sons, NY, NY, 2:1-72. June H.L., Gilpin N.W. (2010). “Operant self-administration models for testing the neuropharmacological basis of ethanol consumption in rats.” In Current Protocols in Neuroscience. John Wiley and Sons, NY, NY 9: 9.12.1-26. Abstracts: June, H.L., & Lewis, M.J. (1986). Pre-exposure Effects of a Self-Administered Drug on a Non-Self-Administered Unconditioned Stimulus. Society for Neuroscience Abstracts, Vol. 12, Part 1. Johnson, L.T., June, H.L., & Lewis, M.J. (1987). The effects of Ro15-4513 on generalized motor activity. Society for Neuroscience Abstracts, Vol. 13, Part June, H.L., Andrade, J.R., & Lewis, M.J. (1987). Effects of feeding schedule: investigation of morphine-induced feeding. Society for Neuroscience Abstracts, Vol. 13, Part 1. June, H.L., Moore, T.O., Edwards, K.L., & Lewis, M.J. (1988). Differential ethanol and habituation effects on open-field activity measures. Society for Neuroscience Abstracts Vol. 14, Part 2. June, H.L., Hicks, L., Lawson, W.B., & Lewis, M.J. (1988). A comparison of the effects of clozapine and haloperidol on drinking behavior in the rat. Proceedings and Abstracts of the Annual Meeting of the Eastern Psychological Association, Vol. 59, p. 46. June, H.L., Lewis, M.J., Ross, S., Colker, R.E., & Hicks, L.H. (1988). A comparison of the effects of clozapine on a cocaine versus a CuSO4-induced conditioned taste aversion in the rat. Proceedings and Abstracts of the Annual Meeting of the Eastern Psychological Association, Vol. 59, p. 49. Lewis, M.J., Bixler, M. A., & June, H.L. (1988). Similar behavioral effects of benzodiazepine inverse agonists. Alcoholism: Clinical and Experimental Research, Vol.. 12, p. 315. Siegert, F.G., June, H.L., Petty, R.M., & Colker, R.E. (1989). The effects of external rewards and teaching styles on the motor performance of mentally retarded students. Proceedings and Abstracts of the Annual Meeting of the Eastern Psychological Association, Vol. 59, p. 37.
17 June, H.L., Hicks, L.H., Lewis, M.J. & Ross, S. (1989). Denatonium saccharide as an aversive stimulus in a conditioned taste aversion paradigm. Proceedings and Abstracts of the Annual Meeting of the Eastern Psychological Association, Vol. 60, p. 50. Moore, T.O., June, H.L., & Lewis, M.J. (1990). Ro15-4513 antagonizes ethanol-induced stimulant effects on measures of exploration in rats. Society for Neuroscience Abstracts Vol. 16, Part 1, p. 435, 1990. Hicks, L.H., June, H.L., Moore, T.O., & Lewis, M.J. (1990). A comparison of three inverse benzodiazepine agonists on ethanol stimulant effects. Society for Neuroscience Abstracts Vol. 16, Part 1, p. 756, 1990. June, H.L., Domangue, K.R., Hughes, R.W., Spurlock, H.L., & Lewis, M.J. (1991). Differential effects of dopamine and serotonin antagonists on ethanol selfadministration. Poster presented at the Research Society on Alcoholism Annual Meeting, Marco Island, Florida, June. June, H.L., Stitzer, M.L., & Liebson, I.A (1991). Antagonist sensitivity time course in humans: effects of morphine-naloxone interval. National Institute of Drug Abuse, Research Monograph, ed. L.S. Harris. No. 92, In Committee on Problems of Drug Dependence U.S. Government Printing Office, Washington, D.C. Domangue, K.R., June, H.L., Smith, T.D., Dixon, J.T., Norona, A., & Lewis, M.J. (1991). Buprenorphine produces biphasic effects on ethanol self-administration. Society for Neuroscience Abstracts Vol. 17, Part 2, p. 1422. June, H.L., & Lewis, M.J. (1991). Ro15-4513 effects on ethanol-induced locomotor activity in alcohol-preferring and non-preferring rats. Society for Neuroscience Abstracts Vol. 17, Part 2, p. 1425. Hughes, R.W., June, H.L., Spurlock, H.L., Dixon, J.T., Hicks, L.H., & Lewis, M.J. (1991) Effects of cocaethylene on oral ethanol self-administration. Society for Neuroscience Abstracts Vol. 17, Part 2, p. 1423 Bixler, M. A., June, H.L., & Lewis, M.J. (1991). Effects of oral ethanol self-administration and Ro15-4513 on wheel running behavior. Society for Neuroscience Abstracts Vol. 17, Part 2, p. 1423. Lewis, M.J., & June, H.L. (1991). Buprenorphine produces biphasic effects on ethanol selfadministration. M.J. Lewis and H.L. June. Poster presented at the American College of Neuropsychopharmacology, San Juan, Puerto Rico, December. June, H.L., & Lewis, M.J. (1991). Effects of cocaethylene on oral ethanol selfadministration. Poster presented at the American College of Neuropsychopharmacology, San Juan, Puerto Rico, December. June, H.L., Preston, K.L., Bigelow, G.E., & Stitzer, M.L. (1992). Effects of buprenorphine and methadone in methadone-maintained subjects. National Institute of Drug Abuse, Research Monograph, ed. L.S. Harris. No. 93, In Committee on Problems of Drug Dependence U.S. Government Printing Office, Washington, D.C.
18 Lewis, M.J., Smith, T., Domangue, K., Bryant, J., & June, H.L. (1992). Locomotor stimulation by oral alcohol self-administration in alcohol preferring (P) rats: blockade by dopamine (DA) and 5-HT3 receptor antagonists. Paper presented at the American College of Neuropsychopharmacology Annual Meeting, San Juan, Puerto Rico, December. June, H.L., Preston, K.L., Marco, A., & Stitzer, M.L (1992). Acute opioid dependence after a single fentanyl exposure in humans. Paper presented at the American College of Neuropsychopharmacology Annual Meeting, San Juan, Puerto Rico, December. Starasto, A.R., Blakley, G.G., June, H.L., & Lewis, M.J. (1994). The benzodiazepine RO166028 (bretazenil) augments the depressants effects of ethanol. Society for Neuroscience Abstracts Vol. 20, p. 1606 Williams, J., June, H.L., Dejaravu, S., & Murphy, J.M. (1994). The benzodiazepine antagonist CGS-8216 exerts prolonged and selective attenuation of ethanol intake in alcohol-preferring (P) rats. Society for Neuroscience Abstracts Vol. 20, p. 1606 Duemler, S., June, H.L., Williams, J., Breen, T.E., Lewis, M.J., & Murphy, J.M. (1994). The benzodiazepine receptor inverse agonist RO19-4603 exerts prolonged antagonism of the locomotor depressant effects of ethanol in alcohol-preferring (P) rats. Society for Neuroscience Abstracts Vol. 20, p. 1606 June, H.L., Greene, T.L., Lin, M., & Murphy, J.M. (1994). Differential sensitivity of the benzodiazepine inverse agonist RO19-4603 on the locomotor depressant effects of ethanol in alcohol-preferring (P) and -nonpreferring (NP) rats. Society for Neuroscience Abstracts Vol. 20, p. 499 Hewitt, R.L., June, H.L., Murphy, J.M., Greene, T.L., Lin, M., Mellor-Burke, J.J., Lumeng, L., & Li, T.-K. (1994). Does tolerance develop to the ethanol suppressant effects of the benzodiazepine receptor inverse agonist, RO19-4603? Alcoholism: Clinical and Experimental Research, Vol. 18, p. 487. June, H.L., Murphy, J.M., Hewitt, R.L., Greene, T.L., Lin, M., Chen, S.H.A., Mellor-Burke, J.J., Lumeng, L., & Li, T.-K. (1994). Benzodiazepine receptor ligands with different intrinsic efficacies alter ethanol intake in alcohol non-preferring (NP) rats. Alcoholism: Clinical and Experimental Research, Vol. 18, p. 487. June, H.L., Chen, S.H.A., Duemler, S., Lin, M. Greene, T.L., Williams, J., Hewitt, R.L., Mellor-Burke, J.J., Lewis, M.J. & Murphy, J.M. (1994). Effects of RO19-4603 on the maintenance of tolerance to a single dose of ethanol. Alcoholism: Clinical and Experimental Research, Vol. 18, p. 609. June, H.L., Murphy, J.M., Lewis, M.J., Lumeng, L., Li, T-K. (1994). Differential sensitivity of the benzodiazepine inverse agonist RO19-4603 on the locomotor depressant effects of ethanol in low and high drinking F2 rats. Paper presented at the American College of Neuropsychopharmacology, San Juan, Puerto Rico, December. June, H.L., Lewis, M.J., Murphy, J.M., Lumeng, L., Li, T-K. (1994). The benzodiazepine antagonists CGS-8216 and ZK 93426 attenuate ethanol intake in alcohol-preferring (P) rats. Paper presented at the American College of Neuropsychopharmacology, San Juan, Puerto Rico, December.
19 June, H.L., Williams, J.A., Cason, C.R., Braun, M., Torres, L. Allen, Murphy, J.M., Lumeng, L., Li, T-K (1995). Bretazenil exerts anxiolytic effects in alcohol-preferring (P) rats. Alcoholism: Clinical and Experimental, Vol. 19, p.74 June, H.L., Williams, J.A., Cason, C.R., Devaraju, M., Lin, M. Murphy, J.M., Lumeng, L., Li, T-K (1995). Low doses of gamma-hydroxybutyric acid (GHB) attenuate ethanol intake in alcohol-preferring (P) rats. Alcoholism: Clinical and Experimental, Vol. 19, p.52 June, H.L., Murphy, J.M., Lewis, M.J. Lumeng, L. & Li, T-K. (1995). The inverse agonist RO19-4603 antagonizes EtOH intake and EtOH reinforced responding in alcoholpreferring (P) rats. Paper presented at the American College of Neuropsychopharmacology, San Juan, Puerto Rico, December. Zuccarelli, D., June, H.L., Durr, L.F., & Murphy, J.M. (1996) Benzodiazepine receptor ligands with different intrinsic efficacies modulate EtOH-reinforced responding in alcohol-preferring (P) rats. Alcoholism: Clinical and Experimental Research. 20:12A-#50 Craig, K., June, H.L., Torres, L. Braun, M.R. & Murphy, J.M. (1996) Benzodiazepine receptor antagonists modulate the behavioral actions of ethanol. Alcoholism: Clinical and Experimental Research 20:12A-#49 Eggers, M.W., June, H.L., Ricks, A., Cason, C.R., & Murphy, J.M. (1996). The novel benzodiazepine inverse agonist RU 34000 and antagonist RU 40410 antagonizes EtOH-reinforced responding: in alcohol-preferring (P) rats. Alcoholism:Clinical and Experimental Research 20:12A-#52 Cason, C.R., June, H.L., Matecka, D. & Murphy, J.M. (1996). Effects of the selective, high affinity ligand ZG-63 for diazepam insensitive subtype of the benzodiazepine receptor on EtOH-reinforced responding. Alcoholism: Clinical and Experimental Research. 20:12A-#51 June, H.L., Murphy, J.M., Lewis, M.J., Froehlich, J., & Lumeng, L. (1996) Preclinical characterization of the 6-methylene derivative nalmefene in alcohol-preferring rats. Paper presented at the annual meeting of the American College of Neuropsychopharmacology, San Juan, Puerto Rico, Dec. 1996. June, H.L., Murphy, J.M., Lewis, M.J., & Lumeng, L. (1996) GABAergic transmission in the mesoaccumbens is involved in alcohol-self administration in alcohol-preferring rats. Paper presented at the annual meeting of the American College of Neuropsychopharmacology, San Juan, Puerto Rico, Dec. 1996. June, H.L., Hwang, B.H., & Murphy, J.M. (1997) GABAergic transmission in the mesoaccumbens is involved in alcohol motivated behaviors in alcohol-preferring (P) and outbred Wistar rats. Paper presented at the Annual European Society for Biomedical Research on Alcoholism in a symposium entitled "Neurobiology of alcohol reinforcement", sponsored by the 6th Congress and Karolinska Institute, Stockholm, Sweden, June 28-July 1, 1997. Alcohol and Alcoholism 32:337.
20 Cason, C.R., June, H.L., Fredericks M., Cheatham, G., Chen, A., Cook, J., Gan, T., & Murphy, J.M. (1997) GABAergic regulation of the reinforcing properties of alcohol in Wistar rats. Society for Neuroscience Abstracts Vol. 23, p. 959 June, H.L., Cheatham, G., Fredericks M., Cason, C.R., Chen, A., Cook, J., Gan, T., & Murphy, J.M. (1997) GABAergic regulation of the sedative properties of alcohol in the striatum of Wistar rats. Society for Neuroscience Abstracts Vol. 23, p. 958 Lewis, M.J., Starosta A.N., & June, H.L. Bretazenil (RO16-6028) blocks ethanol inducedactivation, but does not potentiate the depressant effects of alcohol. American College Neuropsychopharmacology, Kona, Hawaii, Dec. 1997. June, H.L., Cheatham, G., Fredericks M., Cason, C.R., Chen, A., Cook, J., Gan, T., & Murphy, J.M. (1997) GABAA-benzodiazepine receptors in the striatum are involved in the sedation produced by a moderate, but not an intoxicating ethanol dose in wistar rats. American College of Neuropsychopharmacology, Kona, Hawaii, Dec. 1997. June, H.L., McCane, S.L., Williams, L., Grey, C., Rock, S., & Johnson, T.B. (1998) Microinjections of nalmefene, the 6-methylene derivative of naltrexone in the nucleus accumbens and ventral tegmental area reduces responding maintained by ethanol. Behavioral Pharmacology 9:S48
June, H., Grey, C., Johnson, T.B., Williams, L., McKay, P., Rock, S., He, X., & Cook, J.M. (1998). GABAergic substrates in the nucleus accumbens and dorsal hippocampus mediate responding maintained by EtOH presentation in alcohol-preferring (P) rats. Behavioural Pharmacology 9:S47 McCane, S.L. Rock, S., Williams, L., Grey, C., Johnson, T.B. & June, H.L. (1998) Microinjections of nalmefene, the 6-methylene derivative of naltrexone in the nucleus accumbens and ventral tegmental area reduces responding maintained by ethanol. Alcohol Clin Exp Res 22:45A [Abstract # 250] June H.L., McCane S.L., Zink, R.W., Portoghese, P.S., Li, T.-K., & Froehlich, J.C. (1998). Opioid receptor antagonists reduce responding for ethanol. Alcohol Clin Exp Res 22:45A [Abstract # 248] Grey, C., Williams, L., McCane, S.L., Cook, J.M. & June, H. (1998) The neutral 1 s ubtype specific benzodiazepine (BDZ) ligand reduces responding maintained by ethanol under different schedules of availability. Alcohol Clin Exp Res 22:11A [Abstract # 45] June, H.L. & Hwang, B.H. (1998) Prevalence and distribution of the α4 containing GABAA receptor subunit in alcohol- preferring (P) and nonpreferring (NP) rats. Alcohol Clin Exp Res 22:11A [Abstract # 44]. June, H.L., McKay, P.F., Grey, C., Melendez, R.L., Foster, F., Johnson, T.B., He, X. & Cook, J.M (1998). RY 023: A High-Affinity, Selective Ligand for GABAA-Receptor Containing α5-Subunits Attenuates Responding Maintained by Ethanol Following Microinjection Into the CA1 Hippocampal Area. (1998). Paper presented at the
21 American College of Neuropsychopharmacology, Las Crobas, Puerto Rico, December. Foster, K.L., McKay, P.F., Mason, D., Cummings, R., Garcia, M., He, X. Cook, J.M. & June, H.L (1999). RY 023: A Selective GABAA-5-Subunit Ligand Attenuates Responding Maintained by Ethanol Following Microinjection Into the CA1 and CA3 Hippocampal Areas. Alcohol Clin Exp Res 22:16A [Abstract # 38]. Cummings, R., Garcia, M., Mason, D., McKay, P.F., Foster, K.L., & June, H.L (1999). Differential Effects of Unilateral Versus Bilateral Microinjections of Nalmefene in the Nucleus Accumbens and Ventral Tegmental Area in Alcohol Preferring (P) Rats. Alcohol Clin Exp Res 22:16A [Abstract # 62]. McKay, P.F., Mason, D., Cummings, R., Garcia, M., Foster, K.L., He, X. Cook, J.M. & June, H.L (1999). The GABAA-α5-Subunit Mediate the Sedative and Rewarding Effects of Ethanol: Preclinical Studies in Outbred Rats. Alcohol Clin Exp Res 23:12A [Abstract # 39]. June, H.L., McKay, P.F., Foster, K.L., Mason, D., Cummings, R., Garcia, M., He, X. & Cook, J.M (1999). The α5 GABAA-Receptor Subunit in the CA1 and CA3 Hippocamus Regulate Alcohol Responding in “P” Rats. Alcohol and Alcoholism 34:425 [Abstract # 18]. Carroll, M., Foster, K.L., Harvey, S., McKay, P.F., Cook, J.M., & June, H.L. (2000). Selective GABAA-1 s ubunit ligands (e.g., ßCCt, 3-PBC) attenuate responding maintained by ethanol following microinjection into the ventral pallidum. Alcohol Clin Exp Res Alcohol Clin Exp Res 24:53A [Abstract # 242]. Foster, K.L., McKay, P.F., Harvey, S., Cook, J.M., & June, H.L. (2000). GABAergic in the central amygdaloid nucleus regulate alcohol motivated behaviors in alcoholpreferring (P) Alcohol Responding in “P” Rats. Alcohol and Alcoholism 24:47A [Abstract # 241]. Woods, J., McKay, P.F., Lewis, M. J., & June, H. L. (2000). Basal brain stimulation reward (BSR) performance in high alcohol drinking (HAD) and low alcohol drinking (LAD) rat lines. Alcohol Clin Exp Res 24:53A [Abstract # 280]. Yin W., Carroll M., June H.L., & Cook, J.M., (2001). Alcohol’s euphoric properties are regulated by the GABAA-α1 subtype. Paper to be presented at the Committee on Problem and Drug Dependence annual meeting in June 2001. Lewis, M. J., & June, H. L. (2001). Basal brain stimulation reward (BSR) in alcoholpreferring (P, HAD) and non-preferring (NP, LAD) rat lines. Paper presented at the American College of Neuropsychopharmacology, Waikoloa, Hawaii, Dec. 2001. Woods, J., II, Seyoum R., McKay, P.F., Foster, K.L., & June, H.L. (2001). Differential responding for brain stimulation reward (BSR) and sucrose-maintained responding in alcohol preferring (P) and non-preferring (NP) rats. Paper to be presented at the annual meeting of the Research Society on Alcoholism in Montreal, Canada, June, 2001.
22 Woods, J., II, Seyoum R., McKay, P.F., Foster, K.L., & June, H.L. (2001) Brain stimulation reward (BSR) performance in high alcohol drinking (HAD) and low alcohol drinking (LAD) rat lines: Effects of dopamine (DA), opioid, and benzodiazepine (BDZ) antagonist pretreatments. Paper to be presented at the annual meeting of the Research Society on Alcoholism in Montreal, Canada, June, 2001. Foster, K.L., Seyoum R, McKay, P.F.,Cook, J.M., & June, H.L. (2001) GABA and opioid receptors in the in the central amygdaloid nucleus regulate alcohol and sucrose motivated behaviors. Paper to be presented at the annual meeting of the Research Society on Alcoholism in Montreal, Canada, June, 2001. Carroll, M.R., Woods, J., Seyoum, R.A., Cook, J.M., & June, H.L. (2001) The role of the GABAA Îą1 subtype in mediating the sedative and anxiolytic properties of benzodiazepines in high and low alcohol drinking rats. Paper to be presented at the annual meeting of the Research Society on Alcoholism in Montreal, Canada, June, 2001. Hardy, L., Eiler II, W.J.A., Woods, J., Seyoum, R. & June, H.L. (2002) Responding for Brain Stimulation Reward (BSR) in the Bed Nucleus of the Stria Terminalis (BST) in High Alcohol Drinking (HAD) and Low Alcohol Drinking (LAD) Rat Lines. Paper presented at the annual meeting of the Research Society on Alcoholism in San Francisco, CA, June, 2002 Goergen, J., Seyoum, R.& June, H.L. (2002) Dopamine, But Not GABAA Receptors of the Lateral Hypothalamus (LH) Regulate Alcohol-Maintained Responding. Paper presented at the annual meeting of the Research Society on Alcoholism in San Francisco, CA, June, 2002 Woods, J.E., Mc Kay, P.F. & June, H.L. (2002). Responding for Brain Stimulation within the Ventral Tegmental Area Before and After Amphetamine Pretreatment in High Alcohol Drinking (HAD) and Low Alcohol Drinking (LAD) Rat Lines. Paper presented at the annual meeting of the Research Society on Alcoholism in San Francisco, CA, June, 2002 Eiler II, W.J.A., Mailey, C., & June, H.L. (2002). The D1 Dopamine Receptor Regulate Alcohol-Motivated Behaviors in the Bed Nucleus of the Stria Terminalis (BNST) in Alcohol-preferring (P) rats. Paper presented at the annual meeting of the Research Society on Alcoholism in San Francisco, CA, June, 2002. Foster, K.L., Seyoum, R., Goergen, J., Mensah, B. & June, H.L. (2002). GABA/Dopamine Interaction in the Central Amygdala (CeA) Regulate Ethanol-Maintained Responding. Paper presented at the annual meeting of the Research Society on Alcoholism in San Francisco, CA, June, 2002. Woods, J.E., Mc Kay, P.F. & June, H.L. (2002). Responding for Brain Stimulation within the Ventral Tegmental Area Before and After Amphetamine Pretreatment in High Alcohol Drinking (HAD) and Low Alcohol Drinking (LAD) Rat Lines. Paper presented at the annual meeting of the Society for Neurosciences, Orlando, FL November, 2002. Eiler II, W.J.A., Mailey, C., & June, H.L. (2002). The D1 Dopamine Receptor Regulate Alcohol-Motivated Behaviors in the Bed Nucleus of the Stria Terminalis (BNST) in
23 Alcohol-preferring (P) rats. Paper presented at the annual meeting of the Society for Neurosciences, Orlando, FL November, 2002. Foster, K.L., Seyoum, R., Goergen, J., Mensah-Zoe, B. & June, H.L. (2002). GABA/Dopamine Interaction in the Central Amygdala (CeA) Regulate EthanolMaintained Responding. Paper presented at the annual meeting of the Society for Neurosciences, Orlando, FL November, 2002. Hardy III L., Eiler II, W.J.A, Masters, J., Woods III, J.E, & June H.L. (2003). Responding for brain stimulation reward (BSR) in high alcohol drinking (HAD) and low alcohol (LAD) drinking rats following amphetamine and morphine pretreatments. Alcohol Clin Exp Res 27:92A [Abstract # 517]. Goergen, J., Mensa-Zoe, B., June, H.L. (2003) The D2 dopamine receptor regulates ethanol-motivated behaviors in the lateral hypothalamus of alcohol-preferring (P) rats.Alcohol Clin Exp Res 27:92A [Abstract # 519]. Foster, K.L., Goergen, J., Simmons, J., Cook, J.B., Homanics, G., Ferguson, C., June, H.L. (2003). The role of the Îą1 containing GABAA receptor subunits in ingestive behaviors. Alcohol Clin Exp Res 27:85A [Abstract # 475]. Eiler II, W.J.A., Masters, J., Hardy III, L., Cook, J.B., Woods III, J.E., June, H.L., (2003) Alcohol preference predicts amphetamine abuse liability using the brain stimulation reward (BSR) paradigm. Alcohol Clin Exp Res 27:91A [Abstract # 516]. Eiler II, W.J.A., Cook, J.B., Masters, J., Woods II, J.E., Goergen, J., Hardy III, L., Johnson, N., June, H.L. (2004) Brain stimulation reward performance and sucrose maintained behaviors in alcohol preferring (P) and non-preferring (NP) rats. Paper presented at the annual meeting of the Research Society on Alcoholism Cook, J.B., Goergen, J., Mensah-Zoe, B., Eiler II, W.J.A., Hardy III, L., Johnson, N., June, H.L. (2004) Dopamine D2 and opioid receptors in the lateral hypothalamus regulate ethanol-motivated behaviors in the alcohol-preferring rat. Paper presented at the annual meeting of the Research Society on Alcoholism Liu, J., Yang, A.R.S.T., Yi, H.S., Puche, A.C., Sieghart, W., June, H.L., Aurelian, L.. (2008) Excessive alcohol intake is abolished by HSV amplicon delivered GABAA Îą1 siRNA to the ventral pallidum. Presented at the College of Neuropsychopharmacology Dept in Scottsdale, AZ MAJOR INVITED TALKS: Lewis, M.J., Bixler, M.A., June, H.L., Johnson, L.T., & Salmoiraghi, G.C. (1987). Effects of Ro15-4513 and ethanol on measures of spontaneous activity. Paper presented at the American College of Neuropsychopharmacology, San Juan, Puerto Rico, December. Lewis, M.J., & June, H.L. (1988) Ro15-4513 potentiation of ethanol-induced stimulation of activity. Paper presented at the Fourth Congress of the International Society: Biomedical Research on Alcoholism, Kyoto, Japan, July.
24 Dockett, K.H., Milburn, N., June, H.L., & Booth, J.E. (1989). Dispelling the myths of homeless people in Washington, DC. Roundtable discussion conducted at the Annual Southeastern Psychological Association, DC Hilton Hotel, Washington, DC, April. Lewis, M.J., June, H.L., Perry, L.B., Garnette, M., & Porrino, L.J. (1990). Alteration in specific brain activity with stimulant effects of ethanol. Paper presented at the Research Society on Alcoholism Annual Meeting, Toronto, Canada, June. June, H.L., Perry, L.B., Domangue, K.R., & Lewis, M.J. (1990). Ro15-4513 attenuates and enhances consumption of ethanol in deprived rats. Paper presented at the Behavioral Pharmacology Society Meeting, Annapolis, Maryland, May. Lewis, M.J., June, H.L., Perry, L.B., Garnette, M., & Porrino, L.J. (1990). Alteration in specific brain activity with stimulant and depressant effects of ethanol. Paper presented at the Annual Neuroscience Society Meeting, St. Louis, Missouri, November. Lewis, M.J., June, H.L., Perry, L.B., Garnette, M., & Porrino, L.J. (1991). Changes in regional brain activity with behavioral effects of ethanol and blood ethanol concentration. Paper presented at the ACNP Annual Meeting, San Juan, Puerto Rico, December. June, H.L., Hughes, R.W., Spurlock, H.L., Domangue, K.R., & Lewis, M.J. (1991). Ethanol self-administration in freely feeding rats: effects of Ro15-4513 alone and in combination with flumazenil (Ro15-1788). Paper presented at the Research Society on Alcoholism Annual Meeting, Marco Island, Florida, June. June, H.L., Preston, K.L., Bigelow, G.E., & Stitzer, M.L. Buprenorphine effects in methadone maintained subjects (1992). Paper presented at the Committee on Problems of Drug Dependence Annual Meeting, Keystone, Colorado, June June, H.L., Cook, J., Lewis, M.J., & Murphy, J.M. (1997) GABAergic transmission in the mesoaccumbens is involved in alcohol motivated behaviors. Paper presented at the 41st Annual Behavioral Pharmacology Meeting, Ritz Carlton Hotel, Philadelphia, PA, May 1997 June, H.L., Hwang, B.H., & Murphy, J.M. (1997) GABAergic transmission in the mesoaccumbens is involved in alcohol motivated behaviors in alcohol-preferring (P) and outbred Wistar rats. Paper presented at the Annual European Society for Biomedical Research on Alcoholism in a symposium entitled "Neurobiology of alcohol reinforcement", sponsored by the 6th Congress and Karolinska Institute, Stockholm, Sweden, June 28-July 1, 1997 June, H., Grey, C., Johnson, T.B., Williams, L., McKay, P., Rock, S., He, X., & Cook, J.M. (September 1998). GABAergic substrates in the nucleus accumbens and dorsal hippocampus mediate responding maintained by EtOH presentation in alcoholpreferring (P) rats. European Behavioral Pharmacology Meeting, Brno, Czech Republic June, H.L., McKay, P.F., Foster, K.L., Mason, D., Cummings, R., Garcia, M., He, X. & Cook, J.M (June 1999). Τηε α5 GABAA-Receptor Subunit in the CA1 and CA3
25 Hippocamus Regulate Alcohol Responding in “P” Rats. European Society for Biomedical Research on Alcoholism, Barcelona, Spain June H.L., (June, 2002) Alcohol reward is mediated by the GABAA receptor α1 subtype in the ventral pallidum. Guest Lecturer, Howard University Pharmacology Department, Washington, DC June H.L., (August, 2002) Animal Models: Novel Benzodiazepine Pharmacotherapies to Treat Alcohol Abuse, National Medical Association, Honolula, Hawaii June H.L., (September, 12, 2002) Novel Benzodiazepine Pharmaotherapies to Treat Alcohol Abuse: National Alcohol, Tobacco & Drug Addiction Recovery Month, Larue Carter Memorial Hospital, Indianapolis, IN June H.L., (May 2003) Novel Benzodiazepine Pharmacotherapies to Treat Alcohol Abuse, Guest Lecturer, Howard University Pharmacology Department, Washington, DC June H.L., (April 2003) Novel Benzodiazepine Pharmacotherapies to Treat Alcohol Abuse, American Society for Pharmacology and Experimental Therapeutics, San Diego, CA. June H.L., (March 2008) Triple Reuptake Inhibitors Reduce Binge Alcohol Drinking and Negative Affective States of Withdrawal, Howard University School of Medicine Pharmacology Department, Washington, DC June H.L. (April 2010) Binge Alcohol Drinking: In Search of Its Molecular Target via the GABAA Receptor, 5th Annual Leslie H. Hicks Symposium, Howard University Psychology Department, Washington, DC