1 2Table 1. Characteristics of Vibrio paraheamolyticus isolates. 3
Strain no.
Serotype
Year of
Place of isolation tox
Gene profile tdh trh GS-
Allelic profile/ Sequence Type gyrB recA dnaE gnd
isolation AQ 4093 AQ 4901 AQ 4235 AQ 3794 AQ 4037 AQ 3810 AQ 4019
O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6
1986 1993 1987 1983 1985 1983 1985
Maldive Islands Thailand Thailand Singapore Maldive Islands Singapore Maldive Islands
R + + + + + + +
+ -
+ + + + + +
PCR -
1 1 1 1 1 1 1
2 8 2 2 2 2 2
11 2 2 2 2 7 2
1 1 1 1 1 1 1
VP1 VP3 VP4 VP5 VP6 VP8 VP11 VP20
O1:K38 O2:K3 O3:K29 O4:K8 O1:KUT O5:KUT O8:K41 O8:K21
1994 1994 1994 1994 1994 1994 1994 1994
Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta
+ + + + + + + +
+ + + + + + +
+ -
-
9 11 ND 6 10 7 3 2
ND ND 3 ND 3 ND 3 5
12 ND 1 ND 9 7 4 3
8 11 5 2 6 4 2 3
VP23 VP24 VP25 VP27 VP31 VP32 VP33 VP34
O4:K8 O1:K56 O8:K21 O3:K29 O8:K41 O4:K63 O1:K1 O5:KUT
1995 1995 1995 1995 1995 1995 1995 1995
Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta
+ + + + + + + +
+ + + + + + + +
+ -
-
6 1 2 4 3 5 12 7
1 11 ND 3 3 12 10 ND
5 6 3 14 4 10 1 7
2 12 3 5 2 9 13 4
146 2 AN- 13938 OP 411 OP 419 1(1926) FIHES98V1
O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6
1997 1997 1998 1998 1998 1998 1998
Wakayama, Japan Laos ICDDR, B Osaka, Japan Osaka, Japan Kyoto, Japan Fukuoka, Japan
+ + + + + + +
+ + + + + + -
-
+ + + + + + +
1 1 1 1 1 1 1
1 1 1 1 1 1 1
1 1 1 1 1 1 1
1 1 1 1 1 1 1
- 32- 4 VP 26 22/3 AN- 8373 VP 81 DOH 958 15 VP 2 BE98-2062 VP 53
O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6
1998 1998 1998 1996 1997 1998 1998 1996
Thailand Thailand (environment) ICDDR,B Calcutta Taiwan Korea United States Calcutta
+ + + + + + + +
+ + + + + + + +
-
+ + + + + + + +
1 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1
1
1
VP 92 VP 161 VP 162 VP 239 VP 245 VP 266 VP 294 VP 321 VP 362 VP366
O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6 O3:K6
1996 1997 1997 1998 1998 1998 1999 1999 2000 2000
Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta Calcutta
+ + + + + + + + + +
+ + + + + + + + + +
-
+ + + + + + + + + +
1 1 1 1 1 1 1 1 1 1
1 1 4 4 1 1 1 1 1 9
1 1 1 1 1 1 1 1 1 1
1 1 1 1 1 1 1 1 1 1
AN-5034 KX-V483
O4:K68 O4:K68
1998 1997
ICDDR,B Traveler from Thailand,
+ +
+ +
-
+ +
1 1
1 1
1 1
1 1
KX-V508
O4:K68
1997
Kansai Traveler from Nepal,
+
+
-
+
1
1
1
1
KX-V522
O4:K68
1997
Kansai Traveler from
+
+
-
+
1
1
1
1
KX-V532
O4:K68
1997
Singapore, Kansai Traveler from Thailand,
+
+
-
+
1
1
1
1
KX-V563
O4:K68
1998
Kansai Traveler from Thailand,
+
+
-
+
1
1
1
1
KX-V568
O4:K68
1998
Kansai Traveler from Vietnam,
+
+
-
+
1
1
1
1
VP 232 OP 424 KX- V740
O4:K68 O4:K68 O4:K68
1998 1998 1999
Kansai Calcutta Osaka, Japan Traveler from Thailand,
+ + +
+ + +
-
+ + +
1 1 1
1 1 1
1 1 1
1 1 1
VP 237 VP 262 VP293 VP 301
O4:K68 O4:K68 O4:K68 O4:K68
1998 1998 1999 1999
Kansai Calcutta Calcutta Calcutta Calcutta
+ + + +
+ + + +
-
+ + + +
1 1 1 1
1 1 1 1
1 1 1 1
1 1 1 1
VP 185 AN- 16000 VP 250 KX- V737
O1:KUT O1:KUT O1:KUT O1:KUT
1997 1998 1998 1999
Calcutta ICDDR,B Calcutta Traveler from Thailand,
+ + + +
+ + + +
-
+ + + +
1 1 1 1
1 1 1 1
1 1 1 1
1 1 1 1
VP 217 VP 356 VP 378
O1:KUT O1:KUT O1:KUT
1997 2000 2000
Kansai Calcutta Calcutta Calcutta
+ + +
+ + +
-
+ + +
1 1 1
1 1 1
1 1 1
1 1 1
VP 343 VP 367 VP 338 VP339
O1:K25 O1:K25 O1:K25 O1:K25
2000 2000 2000 2000
Calcutta Calcutta Calcutta Calcutta
+ + + +
+ + + +
-
+ + + +
1 1 1 1
1 1 1 1
1 1 1 1
1 1 1 1
1
2
VP331 VP342 VP351 VP381
O1:K25 O1:K25 O1:K25 O1:K25
2000 2000 2000 2000
Calcutta Calcutta Calcutta Calcutta
+ + + +
+ + + +
-
+ + + +
1 1 1 1
1 1 1 1
1 1 1 1
1 1 1 1
VP 105 VP 166 VP 230 VP 333
O1:KUT O1:KUT O1:KUT O1:KUT
1996 1997 1998 2000
Calcutta Calcutta Calcutta Calcutta
+ + + +
+ + +
+ + + +
-
2 5 13 8
5 8 7 6
3 11 13 6
3 10 7 14
1 2Footnotes: GS-PCR refers to group-specific PCR for toxRS. Allelic profile or sequence type is defined as the
3arbitrarily labeled alleles in order (gyrB, recA, dnaE and gnd) following the MLST literature (10, 11, 15, 16, 18, 20, 422, 29). 5
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