Cryogenic Preservation of Animal Colonies: Everything You Need To Know
Cryopreservation, as the procedure is known, allows embryos and sperm to be kept at a low temperature while being utilized to alter a mouse or rat genetically. A freezing agent—glycerol is one example, though corporations are already developing their own unique formulas—is required to cryopreserve cells under colder circumstances. Under the right conditions, embryos and sperm, as well as cell lines, can be kept for long periods.
Cryogenic Preservation of Sperm and Embryos in Lab Animals Has Its Benefits Cryopreservation helps to protect biological material from damage caused by low temperatures or freezing. It also assures that animal research can continue uninterrupted in the event of human or natural calamities that threaten animal colonies. Cryopreservation of gametes and embryos, which contain half of the chromosomes required for reproduction, also prevents genetic drift or minor genetic differences that might affect animal stock. It protects the genetic integrity of valuable strains and provides a feasible alternative to maintaining an active breeding colony, which, as the examples above show, is vulnerable during intense storms. Finally, the genetic stock is easier to transport, disease transmission is reduced, and, perhaps most crucially, stock recovery after a natural disaster is significantly accelerated. You have a lower risk of diseases in a research center if you employ cryopreserved material instead of shipping live mice. While the biological material is clean, the live mice could be vectors that spread disease and contaminate colonies. How Do You Cryopreserve Something? Cryogenic preservation can be done in three different ways. One method uses slow, controlled freezing, while the other uses fast freezing. Verification is a third approach that uses high concentrations of a cry protectant to turn the substance into a glasslike condition, avoiding the formation of ice crystals and thereby preventing embryo harm during the freezing process. The most frequent approach for conserving genetic stock is embryo cryopreservation, the quickest means to rehabilitate a line following a calamity. In order to preserve a line, a minimum of 150-300
embryos are typically frozen. A single straw approximately the size of a pen can hold about 40 embryos. Embryos can be preserved for a long time—as far away from the vivarium as possible to reduce risk— and the genetic material can be quickly delivered hundreds of miles to distant partners. The Bottom Line! At the end of the day, all animal research facilities should have a catastrophe plan that describes the procedures that must be taken to protect their facilities and colonies. They should also think about sorting dishes for better preservation. It could help overcome a natural and human calamity in the long run and save laboratories time and money.
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