What is cryogenic preservation, and how it works?

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What is cryogenic preservation, and how it works? Cells, when kept under low temperature, stop performing its regular biological chemical reactions. Not only cells but Cryopreservation also helps in the prevention of organelles, tissues, sperm, gametes, embryos, tissues, bone marrow, and organ and other such biological constructs. Biological materials are kept in the liquid phase of the liquid nitrogen to preserve complex biological compounds. This is done using two basic techniques -Slow Cooling and Vitrification. Slow freezing provides stability from non-toxic Cyro-protectants, while Vitrification is a fast procedure offering faster recovery and requires no special equipment.


Use of oocyte collection filter in the process of oocyte retrieval Approaches to oocyte retrieval are many. Oocyte collection filter is being practiced to collect the collection of oocytes basically. Picking up oocytes that are most probable to produce is vital for In Vitro Fertilization and Animal Cloning. Brief description of oocyte collection filter • It is specifically designed for the direct collection of an unfertilized ovum. • It adapts to 3 mils or 2 mil needle tubes. • It has a gridded base to facilitate an easy microscope searching • It is Single packaged in a clear bag • It is Sterile • It is a case which can hold up to 100 units


Results The written report recommends that the most eminent oocyte recovery rate happens by applying immense pressures and thicker needles, but this happens at the expense of breaking the cumulusoocyte collection. It is expected that the absolute stress energies create this damage exerted on the cumulus-oocyte system because of the parabolic forces incorporated with the laminar flow inside the needle. During periods of intense flow happening with insertion into the needle, it is likely worsened by uneven forces. More oversized needles also create more major pain and may be connected with more increased blood loss. More powerful velocity insertion within the follicle, needle rotation to stop premature blockage of the lumen & accurately measured uses of aspiration pressure probably optimize oocyte retrieval technique.


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