Kathryn S. Richards, William L. Miller and Eve Marder J Neurophysiol 82:2006-2009, 1999. You might find this additional information useful... This article cites 21 articles, 7 of which you can access free at: http://jn.physiology.org/cgi/content/full/82/4/2006#BIBL This article has been cited by 10 other HighWire hosted articles, the first 5 are: Developmental Regulation of Neuromodulator Function in the Stomatogastric Ganglion of the Lobster, Homarus americanus K. J. Rehm, K. E. Deeg and E. Marder J. Neurosci., September 24, 2008; 28 (39): 9828-9839. [Abstract] [Full Text] [PDF] Spectral Analyses Reveal the Presence of Adult-Like Activity in the Embryonic Stomatogastric Motor Patterns of the Lobster, Homarus americanus K. J. Rehm, A. L. Taylor, S. R. Pulver and E. Marder J Neurophysiol, June 1, 2008; 99 (6): 3104-3122. [Abstract] [Full Text] [PDF]
Removal of GABA within Adult Modulatory Systems Alters Electrical Coupling and Allows Expression of an Embryonic-Like Network E. Ducret, Y. Le Feuvre, P. Meyrand and V. S. Fenelon J. Neurosci., April 4, 2007; 27 (14): 3626-3638. [Abstract] [Full Text] [PDF] Temperature and acid-base balance in the American lobster Homarus americanus S. A. Qadri, J. Camacho, H. Wang, J. R. Taylor, M. Grosell and M. K. Worden J. Exp. Biol., April 1, 2007; 210 (7): 1245-1254. [Abstract] [Full Text] [PDF] Medline items on this article's topics can be found at http://highwire.stanford.edu/lists/artbytopic.dtl on the following topics: Veterinary Science .. Ganglia Updated information and services including high-resolution figures, can be found at: http://jn.physiology.org/cgi/content/full/82/4/2006 Additional material and information about Journal of Neurophysiology can be found at: http://www.the-aps.org/publications/jn
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Journal of Neurophysiology publishes original articles on the function of the nervous system. It is published 12 times a year (monthly) by the American Physiological Society, 9650 Rockville Pike, Bethesda MD 20814-3991. Copyright Š 2005 by the American Physiological Society. ISSN: 0022-3077, ESSN: 1522-1598. Visit our website at http://www.the-aps.org/.
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The pyloric neural circuit of the herbivorous crab Pugettia producta shows limited sensitivity to several neuromodulators that elicit robust effects in more opportunistically feeding decapods P. S. Dickinson, E. A. Stemmler and A. E. Christie J. Exp. Biol., May 1, 2008; 211 (9): 1434-1447. [Abstract] [Full Text] [PDF]
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Maturation of Lobster Stomatogastric Ganglion Rhythmic Activity KATHRYN S. RICHARDS, WILLIAM L. MILLER, AND EVE MARDER Volen Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454-9110
INTRODUCTION
The pyloric rhythm of the stomatogastric ganglion (STG) of adult lobsters and crabs is a highly regular motor pattern that moves the muscles of the stomach. Casasnovas and Meyrand (1995) showed that the STG is present and active by midembryonic development, before the stomach is functional and connected to the hindgut. Early spontaneous and irregular neural activity without obvious functional or behavioral relevance has been seen in many systems and may be important for tuning developing networks (Bradley and Bekoff 1992; O’Donovan 1999; Sillar 1994; Wong 1999). Understanding the role of embryonic and larval rhythms in the maturation of adult circuits requires their quantitative characterization. Therefore we studied the developmental changes in frequency, regularity, and periodicity of the motor discharge of one of the neurons of the pyloric network of the lobster, Homarus americanus, throughout embryonic and larval life. METHODS
The eggs and larvae of H. americanus were obtained from the lobster-rearing facility located at the New England Aquarium. Embryos and larvae were staged as in Helluy and Beltz (1991). The larvae were either fed live or frozen brine shrimp, Artemia salina. Adult animals of both sexes were purchased from local fishermen. Adult stomatogastric nervous systems were dissected from the stomach, pinned in a dish, and superfused with chilled (9 –13°C) saline. Saline composition was as follows (in mM): 479.12 NaCl, The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 2006
12.74 KCl, 10 MgSO4, 3.91 Na2SO4, 13.67 CaCl2, and 5 HEPES, pH 7.45. Extracellular pin electrodes were used to record from the motor nerves (Fig. 1A). The entire stomachs of embryonic and larval animals were dissected and pinned in the dish. Intracellular electrodes were used to record from the p1 muscle, innervated by the LP neuron (Fig. 1A). The p1 excitatory junctional potentials (EJPs) were used as an assay for rhythmic motor pattern generation in the STG (Casasnovas and Meyrand 1995). The intracellular electrodes were filled with 0.6 M K2SO4 and had a resistance of 50 – 80 MV. Data were recorded continuously for 3–20 min. Action potential and EJP times of occurrence were extracted off-line from digitized electrode recordings, using in-house and commercial software (MiniAnalysis, Jaejin). Due to the irregularity of the LP motor activity in most of the embryonic preparations, extraction and analysis of burst onsets and durations was not generally possible. Instead, cycle frequency of the pyloric rhythm was determined from the principal peak of the power spectrum of the action potential or EJP times (Miller and Sigvardt 1998; Rosenberg et al. 1989). For each trial, the corresponding list of event times was divided into three equal-time sections, and the final power spectrum was obtained as an average of the spectra calculated for each section. Each spectrum was normalized to the power at zero frequency to allow comparison of spectra. The full spectra show peaks at high-frequency (20 – 40 Hz) that correspond to the interspike intervals that occur when EJPs are tightly grouped, as in bursts, and peaks at lower frequency that roughly correspond to the frequency of bursts. In this paper we were concerned with the burst frequency but not with the frequency of firing within the burst, and therefore restricted our analyses to the lower frequency range of the spectra. An estimate of the variability in the activity was computed as the ratio of the area under the principal peak region of the power spectrum to the area under the spectrum from 0 to 3 Hz [including the peak region, and with the spectral value at frequency 0 (DC offset) set to 0]. The resulting “power ratio” can range between 0 and 1, is high (.0.5) for highly periodic activity (i.e., most of the power in the signal is found at the cycle frequency of bursting), and decreases with increasing variability in the motor rhythm (i.e., power is found at frequencies other than the cycle frequency). When bursting is highly regular so that the recorded signal approximates a square wave, “harmonic” peaks are seen in the power spectrum at multiples of the peak frequency. These harmonics are much less evident in the spectra of irregular activity. Although it is relatively straightforward to remove the harmonics from highly regular spectra, identification and removal of the harmonics from the cases of irregular activity is not possible. Therefore all of the power ratio calculations include the harmonics, and the relative power in the main spectral peak is underestimated because of the harmonic peaks. RESULTS
Figure 1 shows a schematic of the recording configurations used in this work. Recordings of the adult motor patterns were made extracellularly from the motor nerves. Figure 1B shows the pyloric rhythm from an adult H. americanus. The traces labeled pdn and llvn show the alternate patterns of lateral pyloric (LP), pyloric (PY), and pyloric dilator (PD) neuron
0022-3077/99 $5.00 Copyright © 1999 The American Physiological Society
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Richards, Kathryn S., William L. Miller, and Eve Marder. Maturation of lobster stomatogastric ganglion rhythmic activity. J. Neurophysiol. 82: 2006 –2009, 1999. The stomatogastric ganglion of the adult lobster, Homarus americanus generates extremely regular pyloric rhythms with a characteristic period of 0.5–1.5 Hz. To study the changes in the pyloric rhythm during embryonic and larval development, we recorded excitatory junctional potentials evoked by lateral pyloric (LP) neuron activity. Early in development the motor discharge of the LP neuron was often irregular, preventing use of conventional analysis methods that rely on extracting burst times to calculate cycle frequency and its variability. Instead, cycle frequency was determined for the LP neuron from the peak of the power spectrum obtained from the occurrence times of excitatory junctional potentials in the p1 muscle. The ratio of the power in the peak to the power from 0 to 3 Hz was used as a relative measure of the regularity of the rhythm. Throughout embryonic and the first larval stage, LP neuron activity is slow, irregular, and only weakly periodic. The regularity of the rhythm increased during midlarval stages, and both the frequency and regularity increased considerably by the postlarval stage LIV.
MATURATION OF RHYTHMIC ACTIVITY
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activity. To measure the period and the regularity of the pyloric rhythm we recorded data and performed a Fourier analysis of the spike times extracted from the raw data (see METHODS). Figure 1, C and D, shows the power spectra of the recordings shown in Fig. 1B calculated from the activity of the PD ( pdn recording) and the LP (largest spike on the llvn) neurons, respectively. Both spectra show peaks at the same frequency: 0.46 Hz. The activity of this preparation was highly regular (power ratio of 0.82 for calculated for the PD neuron activity and 0.71 for LP neuron activity). The average cycle frequency in six adult preparations was 0.64 6 0.05 (SE) Hz as measured with both the LP and PD traces. The power ratio was 0.72 6 0.064 measured from the PD activity and 0.61 6 0.031 as measured from the LP neuron activity. These values are not statistically different (P . 0.07), although in all cases the regularity was slightly lower when measured with LP neuron activity than with PD neuron activity, possibly because there are two PD neurons contributing to the PD spectra, thus increasing the signal-to-noise ratio of the power spectrum.
Figure 2 shows raw data and power spectra from individual representative preparations at embryonic and at each larval stage, as monitored by an intracellular recording from the p1 muscle. Unlike the data shown in Fig. 1, in all of these recordings there were episodes in which the LP neuron fired in relatively regular bursts, and other stretches in which it fired quite irregularly. The E75% recording shows an example of a preparation that had several single EJPs, making burst characterization difficult by conventional means. In the recordings shown from the larval times, bursts of EJPs were common, but the bursts were of considerably different durations and had largely variable interburst intervals. The recordings shown for the LIV preparation are considerably more regular than those seen at earlier times. Despite the irregularity of the early rhythms, all of the spectra showed one peak with more power than all the others (preferred peak frequency; Fig. 2). Table 1 presents pooled data for peak frequency and percent power in the peak for embryos, LI, LII, LIII, LIV, and adult animals. Both the frequency and regularity of the rhythm are low during embryonic and the early larval stages and increase as
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FIG. 1. Measuring the frequency of the pyloric rhythm with the power spectrum. A: schematic diagram of the adult stomatogastric nervous system showing the position of the recordings in this paper. The stomatogastric ganglion (STG), was left attached to inputs from the esophageal ganglion (OG) and commissural ganglia (CoGs) via the stomatogastric nerve (stn). The motor nerves indicated include the llvn (low lateral ventricular nerve), the pyn (pyloric nerve), and pdn (pyloric dilator nerve). The position of the p1 muscle, innervated by the lateral pyloric (LP) neuron is indicated. B: simultaneous extracellular recordings made in an adult preparation from the motor nerves at the positions indicated in A. Activity of the LP, pyloric (PY), and pyloric dilator (PD) neurons are indicated. C and D: power spectra calculated from pdn and llvn recordings, respectively, from an adult preparation. F is the frequency of the peak. PR indicates the fraction of the power in the peak region defined by the horizontal bar.
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K. S. RICHARDS, W. L. MILLER, AND E. MARDER
development proceeds. The mean frequency increased by ;2.5fold between embryonic and adult times, whereas the power ratio more than doubled. The actual regularity of adult was most likely greater because the power ratio calculation is preferentially underestimated in the more regular preparations (METHODS). DISCUSSION
Spontaneous rhythmic activity may function to tune both sensory and motor circuits during development (O’Donovan TABLE
1.
Frequency and regularity of the LP neuron discharge
Stage
n
Peak Frequency, Hz
Power Ratio
Embryo LI LII LIII LIV Adult
26 10 7 11 10 9
0.30 6 0.02* 0.38 6 0.04* 0.38 6 0.08* 0.41 6 0.03* 0.50 6 0.07 0.72 6 0.08
0.236 6 0.016* 0.211 6 0.023* 0.319 6 0.029 0.318 6 0.033* 0.410 6 0.041 0.511 6 0.047
Values are means 6 SE; n is number of preparations. The embryonic and larval stage measurements were made using intracellular recordings from the lateral pyloric (LP)–innervated p1 muscle. The adult measurements were made from extracellular recordings of the LP neuron on the llvn. The means were determined to be statistically different by a one-way ANOVA (P , 0.001). Pairwise multiple comparisons (modified Bonferroni test) showed that the embryo, LI, LII, LIII were significantly different from the adult (P , 0.003) and the embryo, LI, and LIII were different from the adult in power ratio (P , 0.003). Additionally, E and LI are significantly different from LIV in power ratio (P , 0.003). * Values significantly different from the adult.
FIG. 3. Possible causes for irregular motor patterns seen early in development. Top panel: left shows the circuit responsible for regular motor patterns in the adult. The commissural pyloric oscillator (CPO) in each commissural ganglion (CoG) drives the pacemaker ensemble (ƒ), which inhibits the LP neuron (●). The LP therefore fires regularly in alternation with the PD neurons. Bottom panel: shows 3 cases, each of which can produce irregular LP neuron activity (see text). AB, anterior burster.
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FIG. 2. LP neuron evoked excitatory junction potentials (EJPs) recorded in the p1 muscle during embryonic and larval times. The left side of each panel shows an intracellular recording from a muscle fiber in the p1 muscle from an animal of the stage indicated. The right side of each panel shows the power spectrum of the event times of the experiment represented to the left. F and PR are as in Fig. 1. Vertical bars, 10 mV. Horizontal bars, 5 s. The resting membrane potentials were 266, 256, 270, 266, and 269 mV, respectively.
1989; Wong et al. 1993, 1995). In particular, irregular activity that precedes functional behavior could be important in the formation of synaptic connections, and in modifying the strengths of synapses and the intrinsic properties of the neurons within a circuit (O’Donovan 1999). Casasnovas and Meyrand (1995) showed that the embryonic STG is rhythmically active, well before the stomach is processing food. Here we demonstrate that this activity is quite irregular, only slightly periodic, and is slower than the adult pyloric rhythm. It is possible that these early and irregular activity patterns may provide maturational signals that allow the STG to coordinately tune its synaptic and intrinsic properties. In this study we used the activity in the muscle innervated by the LP neuron as an assay of the rhythmic activity in the pyloric region of the stomach. In our adult recordings, the frequency of the rhythm obtained with the LP and PD neurons was always identical, and the regularity of the rhythm seen with the LP neuron activity was statistically indistinguishable from that seen with the PD neurons. Figure 3 outlines a number of possibilities that could account for the lower frequency and more variable rhythms seen in the early developmental stages. Figure 3, top panel, illustrates the circuit that is responsible for the adult pyloric rhythm in H. gammarus and almost certainly as well in H. americanus (Cardi and Nagy 1994; Nagy and Cardi 1994; Nagy et al. 1994; Robertson and Moulins 1981a,b). The commissural pyloric oscillator (CPO) network is found in each commissural ganglion (CoG; Fig. 1) and projects to the STG where it rhythmically drives and entrains the pyloric network (Cardi and Nagy 1994; Nagy and Cardi 1994; Nagy et al. 1994; Robertson and Moulins 1981a,b). The rhythmically active PD and anterior burster (AB) neurons inhibit the LP neuron, which is thus entrained to fire in tight pyloric-timed bursts (Fig. 3, adult). Therefore in the adult, the frequency (measured either from the LP or the PD neurons) will be a consequence of the frequency of the entraining CPO. The three cases shown in Fig. 3 illustrate that the lower frequencies and lower regularity seen early in development can result from variability occurring at a number of different sites in the circuit. In Case 1, the CPO and the pacemaker ensemble may or may not be regular, but the LP neuron fails to follow its rhythmic drive, either because of its intrinsic properties or
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We thank C. Soto-Trevin˜o for help early on in this project and L. F. Abbott, X. J. Wang, M. Goldman, and K. Kemptes for useful discussions on data analysis and statistics. This research was supported by National Institute of Neurological Disorders and Stroke Grant NS-17813 to E. Marder, Individual National Research Service Award NS-10770 to K. S. Richards, and the W. M. Keck Foundation. Address for reprint requests: E. Marder, Volen Center, MS 013, Brandeis University, 415 South St., Waltham, MA 02454-9110. Received 29 April 1999; accepted in final form 22 June 1999. REFERENCES BRADLEY, N. S. AND BEKOFF, A. Development of coordinated movement in chicks. II. Temporal analysis of hindlimb muscle synergies at embryonic day 10 in embryos with spinal gap transections. J. Neurobiol. 23: 420 – 432, 1992.
CARDI, P. AND NAGY, F. A rhythmic modulator gating system in the stomatogastric nervous system of Homarus gammarus. III. Rhythmic control of the pyloric CPG. J. Neurophysiol. 71: 2503–2516, 1994. CASASNOVAS, B. AND MEYRAND, P. Functional differentiation of adult neural circuits from a single embryonic network. J. Neurosci. 15: 5703–5718, 1995. FACTOR, J. R. Biology of the Lobster, Homarus americanus. San Diego, CA: Academic, 1995, p. 528. F´ENELON, V., CASASNOVAS, B., FAUMONT, S., AND MEYRAND, P. Ontogenetic alteration in peptidergic expression within a stable neuronal population in lobster stomatogastric nervous system. J. Comp. Neurol. 399: 289 –305, 1998. F´ENELON, V., KILMAN, V. L., MEYRAND, P., AND MARDER, E. Sequential developmental acquisition of neuromodulatory inputs to a central pattern generating network. J. Comp. Neurol. 408: 335–351, 1999. HARRIS-WARRICK, R. M. AND MARDER, E. Modulation of neural networks for behavior. Annu. Rev. Neurosci. 14: 39 –57, 1991. HELLUY, S. M. AND BELTZ, B. S. Embryonic development of the American lobster: quantitative staging and characerization of an embryonic molt cycle. Biol. Bull. 180: 355–371, 1991. KILMAN, V. L., F´ENELON, V., RICHARDS, K. S., THIRUMALAI, V., MEYRAND, P. S., AND MARDER, E. Sequential developmental acquisition of cotransmitters in identified sensory neurons of the stomatogastric nervous system of the lobsters Homarus americanus and Homarus gammarus. J. Comp. Neurol. 408: 318 –334, 1999. MARDER, E. AND CALABRESE, R. L. Principles of rhythmic motor pattern generation. Physiol. Rev. 76: 687–717, 1996. MILLER, W. L. AND SIGVARDT, K. A. Spectral analysis of oscillatory neural circuits. J. Neurosci. Methods 80: 113–128, 1998. NAGY, F. AND CARDI, P. A rhythmic modulatory gating system in the stomatogastric nervous system of Homarus gammarus. II. Modulatory control of the pyloric CPG. J. Neurophysiol. 71: 2490 –2502, 1994. NAGY, F., CARDI, P., AND COURNIL, I. A rhythmic modulatory gating system in the stomatogastric nervous system of Homarus gammarus. I. Pyloric-related neurons in the commissural ganglia. J. Neurophysiol. 71: 2477–2489, 1994. O’DONOVAN, M. J. Motor activity in the isolated spinal cord of the chick embryo: synaptic drive and firing pattern of single motorneurons. J. Neurosci. 9: 943–958, 1989. O’DONOVAN, M. J. The origin of spontaneous activity in developing networks of the vertebrate nervous system. Curr. Opin. Neurobiol. 9: 94 –104, 1999. ROBERTSON, R. M. AND MOULINS, M. Control of rhythmic behaviour by a hierarchy of linked oscillators in crustacea. Neurosci. Lett. 21: 111–116, 1981a. ROBERTSON, R. M. AND MOULINS, M. Oscillatory command input to the motor pattern generators of the crustacean stomatogastric ganglion. I. The pyloric rhythm. J. Comp. Physiol. 143: 453– 463, 1981b. ROSENBERG, J. R., AMJAD, A. M., BREEZE, P., BRILLINGER, D. R., AND HALLIDAY, D. M. The Fourier approach to the identification of functional coupling between neuronal spike trains. Prog. Biophys. Mol. Biol. 53: 1–31, 1989. SILLAR, K. T. Synaptic specificity: development of locomotor rhythmicity. Curr. Opin. Neurobiol. 4: 101–107, 1994. WONG, R. O., CHERNJAVSKY, A., SMITH, S. J., AND SHATZ, C. J. Early functional neural networks in the developing retina. Nature 374: 716 –718, 1995. WONG, R. O., MEISTER, M., AND SHATZ, C. J. Transient period of correlated bursting activity during development of the mammalian retina. Neuron 11: 923–938, 1993. WONG, R.O.L. Retinal waves and visual system development. Annu. Rev. Neurosci. 22: 29 – 47, 1999.
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because the synapses from the PD and AB neurons to the LP neuron are not properly tuned. In this case, the frequency extracted from the LP neuron activity could be different from that of the CPO and the PD neurons. In Case 2, the CPO may be strongly rhythmic, but unable to drive reliably the PD/AB ensemble, either because the projection to the STG is not present, the synapses are not properly tuned or because the AB/PD neurons do not have the appropriate intrinsic properties. In this case, the PD neurons (and therefore the LP neuron) may have a lower frequency than that of the CPO and may appear irregular. In Case 3, the CPO itself may not be regular or may be absent. The slow and irregular patterns produced by the LP neuron would therefore be indicative of lack of strong rhythmicity in the entire circuit. In addition to the CPO, there are a large number of other modulatory inputs to the STG, which may modify both the intrinsic properties of neurons and the synaptic connections among them (Harris-Warrick and Marder 1991; Marder and Calabrese 1996). Although some of the neuromodulatory inputs are already present by mid-embryonic development, others are not present until late in larval life (Fe´nelon et al. 1998, 1999; Kilman et al. 1999). Therefore the irregularity and low frequency of the early rhythms may be a consequence of the modulatory environment seen early in development. Although the stomach starts to process food at LI, its full mechanical and structural properties develop slowly between LI and LIV (Factor 1995), during which time the remaining modulatory inputs to the STG first become apparent (Fe´nelon et al. 1999; Kilman et al. 1999). The increase in frequency and regularity in late larval and postlarval animals may occur because this is the time at which the activating modulatory inputs are fully present and active. Alternatively, the major changes in both frequency and regularity reported here could reflect developmental processes that tune both the intrinsic properties of circuit neurons and the synaptic connections among them.
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