Florida Department of Law Enforcement STR SUMMARY SHEET
(Ft,LE
Case Number:
20120406772
S amp l e ID
3
swab casings
Description Run
0851179 021511
.
.
31-Jan-13 nsd nsd
075820
nsd
0351358
nsd
C5F1PO
nsd
TH01
nsd
0165539
nsd
0135317 0251338
nsd nsd
0195433
nsd
TPOX
nsd
vWA
nsd
018551
nsd
055818
nsd
AMEL FGA
Date:
nsd nsd
Sample ID Run
0851179 021511
075820
C5F1PO
0351358 TH01
0135317
0165539 0251338
0195433 vWA
TPOX
018551 AMEL
055818 FGA
Approved 01 May 2012
02/11/13
-:-··
Initials:
FDLE#:
D2te:J
I
Date:
--
spot I tube I
overiayf
a-napt
�-
\�
FDLE Case #:
�o \d-. oL-io l_p T--:=t �
Area Cleaned
P3n I HEMATRACE
sf
Po
Oitho-di'l.: 1 .
.,
I
I$$Uet! 'from 8WN10 March 25, 20i0
Date:/
Fcr.P30:
Ex!;. Da:e: .
I
.
.
PHADEBAS.
1:50j
K! Lot[
Ser.le!i
:
.
O.SM ..
... . .. · -·
. .. . -
Florida Department of Law Enforcement Biology Section
FD ·.
Date:
.
C;i
SAMPLE PREPARATION WORKSHEET DNA IQ™ LYSIS BUFFER METHOD- NON-DIFFERENTIAL
...
.
/
Area:
Analyst:
/
K
Cleaned:
-Vol. Case # I Comments
Sample I D I Description
lsw20120406772_Ex3
lsw20120406772_RBQ
1t
(IJL)
Sample I D I Description
Case # I Comments
-Vol. (�L)
lsw20120406045 Ex1 lsw20120406045 RBQ
lsw20120406801_Ex1
lsw20110404779_Ex9.1
lsw20120406801_Ex2
lsw20110404779_Ex9.2
lsw20120406801_Ex5
lsw20110404779_RBQ
J.
lsw20120406801_Ex6 lsw20120406801_Ex7
?.
lsw20120406801_Ex8
-- :·-1 ; '
lsw20120406801_RBQ
Initials 1
Place the sample cutting into a sterile extraction tube.
2
3 4a
. ..;;) Add 1001JL DNA IQ™ Lysis Buffer with DIT . to the sample tube. "7) Vortex and b �iefly centrifuge to force the cutting into the buffer.
I
/
4b
� 6
Temperature: Start Time:
Incubate in a dry heat block at 95°C for approximately 30 minutes.
Stop Time: Temperature:
General
..
----;. 0
'10
Start Time:
Incubate in a dry heat block at 70°C for approximately 30 minutes to overnight.
Stop Time:
.:.:-s
, ·:-1
:
r
.
Allow the sample to reach room temperature and briefly centrifuge to remove any condensation from the cap.
.;!f\
2
Optional: Transfer the substrate into a spin basket. Centrifuge at maximum speed for
Remove the basket from the extract tube and retain the substrate.
Date:
I
Analyst:
Microcon Lot:
I
Area:
·,�0
�
\ � G\l
Cleaned:
DNA IQ™ Lysis Buffer with OTT Lot:
Comments:
I·
Note: Samples can be left in the buffer for a maximum of 48 hours prior to the start of automated extraction by the BIOMEK® 3000.
-
·;
IQ™ Lysis Buffer with OTT Lot:
FTA Cards
Optional: DNA Concentration I Wash
B
DNA
Pipette a maximum of 100�L of the sample lysate into the
. .
.
appropriate wells of a 96 deep-well plate.
BIO PreProcNonDiff2col (14)1
Time I Date Prepared: Time I Date Loaded�
I
I
:
Issued from BEW1 o March 25, 201 o
(-
----
·-
-- -- ---·-- --
CFDLE
--
--
-
-. --
---- -
-
Florida Department of Law Enforcement Biology Section
Biomek® 3000 Extraction Set
Description
Sample ID
-
Case #I Comments
Sample
KIQ
Date:
B3K Plate Name:
ID
Description
30-Jan-13
Case # I Comments
KIQ
1 lsw:20120406772 Ex3
Q
41 edmh 20120407111 3
Q
2 lsw20120406772_RBQ
Q
42 edmh_20120407111 4
Q
3 lsw20120406801_Ex1
Q
43 edmh_20120407111 RBQ
Q
4 lsw20120406801_Ex2
Q
44 edmh_20120407113_1
Q
5 lsw20120406801_Ex5
Q
45 edmh_20120407113_2
Q
6 �w20120406801 Ex6
Q
46 edmh_20120407113_RBQ
Q
7 lsw20120406801_Ex7
Q
47 edmh_20120402713_4
Q
8 lsw20120406801 Ex8
Q
48 edmh_20120402713_RBQ2
Q
9 lsw20120406801 RBQ
Q
49 edmh_20130400029 1
Q
1 0 lsw20120406045 Ex1
Q
5 0 edmh_20130400029_2
Q
1 1 lsw20120406045 RBQ
Q
5 1 edmh 20130400029 3
Q
1 2 lsw20110404779_Ex9.1
Q
5 2 edmh_20130400029_4
Q
1 3 lsw20110404779_Ex9.2
Q
5 3 edmh_20130400029 RBQ
Q
1 4 lsw20110404779 RBQ
Q
54 edmh_20120407111 5 FTA
K
1 5 lsw20090401384_Ex2.3
K
55 edmh_20120407111_RBQ_FTA
K
K
5 6 edmh_20120407111 6
K
K
57 edmh_20120407111_RBK
K
K
5 8 edmh_20120407113_3
K
1 9 lsw20120406801_Ex3
K
5 9 edmh_20120407113_4
K
2 0 lsw20120406801_RBK-FTA
60 edmh_20120407113_5
2 1 lsw20120406801 Ex4
K K
61 edmh 20120407113 RBK
22 lsw20120406801_RBK
K
23. ·lsw20120406045 Ex2
K
63 lsw20130400493 RBQ
24. . lsw20120406045 RBK 25 ·I�W20120406771_Ex1.1
K
64 empty1
K
65 jtm20120404427 2 mn2
plexor only
Q
26 lsw20120406771_RBK
K
66 jtm20120404427_RBQ mn2
plexor only
Q
1 6 lsw20090401384_RBK-3 1 7 lsw20120406645_Ex6
1 8 lsw20120406645_RBK
K
67
28 lsw20120404249_RBK
K
68
29 lsw20120406578_Ex1
K
69
30 lsw20120406578_RBK
K
70
31 lsw20120406998 Ex1
K
71
32 lsw20120406998 RBK
K
72
33 lsw20120407000_Ex1
K
73
34 lsw20120407000 RBK
K
74
Q
76
Q Q
75
36 lsw20120406771_Ex1.2epi 37 lsw20120406771_RBQsp
Q
77
3,8 lsw20120406771_RBQepi
Q
78
·.
39 edmh_20120407111_1
Q
79
40 edmh_20120407111_2
Q
80
/\II II Dill./ n-:rto
K
62 lsw20130400493 Ex2
27 lsw20120404249_Ex5
35 lsw20120406771_Ex1.2sp
K
nrinin�k �rR m�int::�inP.d in the laboratory
�
p
CFDLE
Florida Department of Law Enforcement Biology Section
™ DNA IQ Lot#:
BIOMEK® 3000 Sample Run Worksheet for DNA IQ
™
End Column:
and Plexor HY ®
30-Jan-13
DNA IQ Date Run:
0000036314
1X Wash Buffer lot#:
Sample Plate was centrifuged prior to removal of sealing film
Time Run:
&
orientation on deck verified by:
AM
rf
Operator- DNA IQ:
B-158
1
rf rf
7500A
7500 Instrument#: 10
60
B-158
Operator- Quant:
20300
B3K Robot#:
23 Oct 2 0 15
Exp Date: Elution Vol:
Lysis Buffer w/ OTT lot#:
AR30Jan13
B3K Plate Name:
8
11
12
A
B
c
D
E
F
O«J4779_
G
H
RBQ
Ex6
Ex7 H1
Ex2.3 H2
ExB
edmh 201JJ4071 RBK-3
11_6
1.5ml Rack
1
Comments:
A9
Plexor
&
®
1.5ml Rack 4
1.5ml Rack 3
89-plexor only, to be added by operator
HY Lot#:
TE
�
1 . 5ml Rack 2
empty1
-4
Lot#:
0000042914 8-51 66
Exp Date:
10/22/2013
DNA Standard Lot#:
0000042914
Total Extracted Samples:
1.5ml Tubes for Transfer Method Verified by:
rf
1-
631
CFDLE Autosomal (H)
Florida Department of Law Enforcement
Biomek®
Plexor® HY Results
H- (20 to 21 )
H- (28 to 29}
Exp. Date:
yes
Y- (21 to 22}
Cr value Std. E & E1
yes yes
I D+ Kit Lot #:
yes
Crvalue Std. B & 81 (1 0ng)
yes
Thermal Cycler:
yes
9700 #:
OveraiiiPC Cr Grouped
TmValue
H- (79-81°C)
::.yes *lPG out of range:
TE""' Batch #:
- -
Quant Pit Name:
PHY AR30Jan 13 7500A RF
Quant evaluator:
E DMH Std G 11 omitted
. . . . .. . . .
:
_ __,
.
.
.
:.·
..
..
.
.
deleted black flags
.
Sample Name/ Sample ID
H Quantity (ng/J!l)
Y Quantity (ng/�1)
[Auto]/M
#of
A1
lsw20120406772 Ex3
0.0000
0.0000
0.00
81
lsw20120406772 RBQ
0.0006
0.0000
0.00
2
C1
lsw20120406801 Ex1
1.7800
1.0500
1.70
3
01
lsw20120406801 Ex2
0.4000
0.0390
10.30
4
E1
lsw20120406801 ExS
0.0053
0.0007
7.50
5
0.0170
0.0011
15.40
6
0.0055
0.0004
13.80
7
0.0259
0.0006
41.90
8
0.0000
0.0000
0.00
9
0.0482
2.00
10
F1
lsw20120406801 Ex6
G1
lsw20120406801 Ex?
H1
lsw20120406801 Ex8 lsw20120406801 RBQ
0.0966
A2
.
-
.
....�Rurce Well
. �
A5
Plexor® HY Results
..
82
lsw20120406045 Ex1
C2
lsw20120406045 RBQ
D2
lsw20110404779 Ex9.1
E2 F2 G2
lsw20090401384 Ex2.3
H2
lsw20090401384 RBK-3
. .. ,..
A3
lsw20120406645 Ex6
B3
lsw20120406645 RBK
0.0000
0.0000
0.00
18
.
C3
lsw20120406801 Ex3
2.0400
1.1800
1.70
19
0.0000
0.0000
0.00
20
4.1200
0.0000
0.00
21 22
03 .' . E3
. .'!·� · :
···
.
. -�
-
B-51
Number of Samples to be Amplified:
02,
..
3
N/A
��- Melt (Tml out of N/A . . range: ..
• .·..
1209050 04 Oct 2013
1-15 13
-(79 81°C)
,. -v·- ·
Comments:
AR30Ja n13
Calibration date:
Y- (81-B3°C)
IPC Melt CurveTM
lsw edmh
yes
(0.08ng)
Y- (29 fo 30)
0.0039
0.0033
1.20
11
25.3000
22.9000
1.10
12
lsw20110404779 Ex9.2
0.1250
0.0478
2.60
13
lsw20110404779 RBQ
0.0000
0.0000
0.00
14
9.9900
4.5200
2.20
15
0.0000
0.0004
0.00
lsw20120406801 RBK-FTA lsw20120406801 Ex4
4.8000
2.3300
2.10
16 17
F3
lsw20120406801 RBK
0.0000
0.0000
0.00
G3
lsw20120406045 Ex2
8.8200
6.6200
1.30
23
H3
lsw20120406045 RBK
0.0000
0.0000
0.00
24
A4
lsw20120406771 Ex1.1
13.4000
0.0006
23848.90
25
--·-
30-Jan-13
yes
IPC Verified
yes yes
f.lrr-
yes
R2
yes
83K Plate Name:
rf
-
yes
(H -23-25) Y-lntercept (Y-24-26)
yes
3000 Amplification Worksheet
---- -
Male (Y)
Slope H- {-3 . 3 to -4.0) Y-·(-3.0 to �3.6)
yes yes
j
Biology Section
Operator: Analyst(s): Date Run:
65
· ----
--·-
---·---
Plexor® HY Results
.
. ,
-
·
�
· ·
.
.
.
.
.
.. .. .
27
C4 04
lsw20120404249 RBK
04
lsw20120404249 RBK
0.0000
0.00
28
E4
0.0000
0.0000
lsw20120406578 Ex1
7.5000
.
-- ·- · ·
.
.
·- ·. -. .. ..
..
.
.
.
.
.
5.3300
lsw2012040657B Ex1
7.5000
4.6400
1.60
29
F4
0.0000
0.0000
0.00
30
lsw20120406578 RBK
lsw20120406578 RBK
G4
lsw20120406998 Ex1
G4
lsw20120406998 Ex1
10.1000
0.0000
0.00
31
H4
lsw20120406998 RBK
lsw20120406998 RBK
0.0000
0.0000
0.00
32
0.0000
H4
85
lsw20120407000 RBK
A5
lsw20120407000 Ex1
19.5000
0.0000
0.00
33
0.0000
C5
6.0300
lsw20120407000 RBK
0.0000
0.0000
0.00
34
lsw20120406771
B5
05
lsw20120406771
12.4000
0.0000
10.1000
6.0300
6.0900
1.00
35
E5
lsw20120406771
12.4000
0.1040
119.90
35
F5
0.0000
lsw20120406771
0.0000
E5
lsw20120406771
0.0000
0.0000
0.00
37
G5
0.0000
0.0000
0.00
H5
edmh 20120407111 1
lsw20120406771
38
0.0385
F5
0.0660
G5
edmh 20120407111 1
0.0385
0.0051
7.50
39
edmh 20120407111 2
A6
0.0108
edmh 20120407111 2
0.0660
0.0115
5.70
40
edmh 20120407111 3
H5
B6
0.0846
A6
edmh 20120407111 3
0.0108
0.0055
2.00
edmh 20120407111 4
41
C6
edmh 20120407111 RBQ
0.0000
B6
edmh 20120407111 4
0.0846
0.0502
1.70
42
06
edmh 20120407113 1
0.0135
C6
edmh 20120407111 RBQ
0.0000
0.0000
0.00
43
E6
edmh 20120407113 2
0.0043
06
edmh 20120407113 1
0.0135
0.0003
48.10
44
F6
edmh 20120407113 RBQ
0.0000
E6
edmh 20120407113 2
0.0043
0.0005
8.50
45
G6
edmh 20120402713 4
0.3300
F6
edmh 20120407113 RBQ'
0.0000
0.0000
0.00
46
H6
edmh 20120402713 4
0.3300
0.2610
1.30
47
edmh 20120402713 RBQ2
G6
A7
0.0000
edmh 20130400029 1
2.8900
H6
edmh 20120402713 RBQ2
0.0000
0.0000
0.00
48
B7
edmh 20130400029 2
0.4370
A7
edmh 20130400029 1
2.8900
3.0900
0.90
49
C7
edmh 20130400029 3
0.0207 0.5830
B7
edmh 20130400029 2
0.4370
0.4850
0.90
50
07
edmh 20130400029 3
0.0207
0.0095
2.20
edmh 20130400029 4
C7
51
E7
edmh 20130400029 RBQ
0.0000
07
edmh 20130400029 4
0.5830
0.7660
0.80
52
F7
edmh 20120407111 5 FTA
0.1160
edmh 20120407111 RBQ FTA edmh 20120407111 6
E7
edmh 20130400029 RBQ
0.0000
0.0000
0.00
53
G7
F7
edmh 20120407111 5 FTA
0.1160
0.0241
4.80
54
H7
G7
edmh 20120407111 RBQ FTA
0.0073
0.0078
0.90
55
AB
edmh 20120407111 RBK
0.0000
H7
edmh 20120407111 6
2.9700
2.1500
1.40
56
BB
edmh 20120407113 3
2.3100
0.0073 2.9700
AB
edmh 20120407111 RBK
0.0000
0.0000
0.00
57
CB
edmh 20120407113 4
0.5540
BB
edmh 20120407113 3
2.3100
0.0000
0.00
58
DB
edmh 20120407113 5
4.6300
edmh 20120407113 RBK
CB
edmh 20120407113 4
0.5540
0.0000
0.00
59
EB
DB
edmh 20120407113 5
4.6300
0.0000
0.00
60
FB
EB
edmh 20120407113 RBK
0.0000
0.0000
0.00
61
GB
lsw20130400493 RBQ
0.0000
FB
lsw20130400493 Ex2
0.0005
0.0000
0.00
62
A12
APC AR30Jan13
0.2510
GB
lsw20130400493 RBQ
0.0000
0.0000
0.00
63
B12
ANC AR30Jan13
#N/A
#N/A
0.0000
#N/A
64
HB
.
lsw20120404249 Ex5
F4
lsw20120406771 Ex1.2sp
·
26
0.00
E4
lsw20120406771
..
•
2.70
05
:,.,
.
0.0000
1.9500
C5
, ·
·
0.0000
5.3300
0
··
..
lsw20120406771 RBK lsw20120404249 Ex5
.
• •
·
84 C4
lsw20130400493 Ex2
0.0000 0.0005
A9
2 mn2
0.0170
0.0124
1.40
65
A9
2 mn2
0.0170
B9
RBQ mn2
0.0000
0.0000
0.00
66
B9
RBQ mn2
0.0000
C9
67
09
68
E9
69
F9
70
G9
71
H9
72
A10
73
B10
74
C10
75
010
76
E10 F10
78
77
G10
79
H10
80
A12
APC AR30Jan13
0.2510
n/a
nla
81
B12
ANC AR30Jan13
0.0000
n/a
n/a
82
AR30Jan13
Date:
Paae 2
- -
101-1l lnput
DNA+
--
---
---
--
----
-----
-
-
-
---
路 --
-
15j.11 Master Mix= 251-11 Amps
(ID+-1 0 f.d total input DNA)+
(1 01-11 extra volume for robotic pipetting) = 20J.ll total input DNA
Number of Samples to be Amped:
Normalization End Column #:
65 9
P50 Setup is used if volume is between2.0J.I and 10.9J.I P200 Setup is used if volume is over 11.0f-LI
Verify that TE volumes do not exceed 11 Q.I or 230J.!I Analyst(s):
lsw edmh
83K Plate Name: TE vol
ijl.l)
AR30Jan13 X
1.0 1.0 2.4
1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 10.0 1.0 6.4
1.0 2.7 1.0 5.5
1.0 8.8
1.0 8 .9
1.0 7.1 1.0 10.0 1.0 10.1 1.0 1.0 8.0 8 .3
1.0 1.0
Date: 30-Jan-13
-
---
- --
--
---
-
-
-
-- -
--
---
--
X
1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 3.9 1.0 1.0 1.0 1.0 1.0 1.0 4.0 1.0 3.1 1.0 6.2 1.0 1.0 1.0 1.0 1.0 1.0 1.0
83K Plate Name:
AR30Jan13
Analyst(s}: P�nP.?
lsw edmh
Date: 30-Jan-13
�,dLM0�11-?-
·
-
-
--
- ·
Ext Pit
Source
P1
A1
P1
-
--r--E--
Norm Pit
New Well
lsw201 20406772..;.;Ex3
P3
A1
0.0
0.0
81
lsw201 20406772_RBQ
P2
P3
81
0.0
0.0
P2
P1
A1
C1
lsw201 20406801 _Ex1
P3
C1
2.0
0.0
P2
P1
01
A1
lsw201 20406801 _Ex2
P3
01
3.8
0.0
P1
E1
P2
lsw201 20406801 _Ex5
A1
P3
E1
0.0
0.0
P2
A1
P2
A1
Sample Name
Well
-
·
· ·
-
-
-
-
vol (ul)
TE _vol
Reservoir
-
-
TEwell A1
P1
F1
lsw201 20406801 _Ex6
P3
F1
0.0
0.0
P1
G1
lsw201 20406801 _Ex7
P3
G1
0.0
0.0
P1
H1
lsw201 20406801 _Ex8
P2
A1
P3
H1
0.0
0.0
P2
A1
0.0
0.0
P2
A1
82
0.0
4.5
P2
A1
A2
P1
A2
lsw201 20406801 _R8Q
P3
P1
82
lsw201 20406045_Ex1
P3
P1
C2
lsw201 20406045_R8Q
P3
C2
0.0
0.0
P1
P2
E2
lsw201 1 0404779_Ex9.2
A1
P3
02
0.0
8.0
P2
A1
P1
F2
lsw201 1 0404779_R8Q
P3
E2
0.0
0.0
P1
P2
G2
lsw20090401 38 4_Ex2.3
A1
P3
F2
2.0
0.0
P2
A1
P3
G2
0.0
0.0
P2
A1
P3
H2
2.0
0.0
P2
A1
P1
H2
P1
A3
I sw20090401 384_RBK-3
lsw201 20406645_Ex6
P1
83
lsw201 20406645_RBK
P3
A3
0.0
0.0
P1
C3
lsw201 20406801 _Ex3
P2
A1
P3
83
2.0
0.0
P2
A1
P1
03
lsw201 20406801 _RBK-FTA
P3
C3
0.0
0.0
P2
P1
A1
E3
lsw201 20406801 _Ex4
P3
03
2.0
0.0
P1
P2
A1
F3
lsw201 20406801 _R8K
P3
E3
0.0
0.0
P2
A1
P1
G3
lsw201 20406045_Ex2
P3
F3
2.0
0.0
P2
A1
P1
H3
lsw201 20406045_R8K
P3
G3
0.0
0.0
P1
P2
A4
lsw201 20406771 _Ex1 .1
A1
P3
H3
2.0
0.0
P2
A1
P1
84
lsw201 20406771 _R8K
P3
A4
0.0
0.0
P1
C4
lsw201 20404249_Ex5
P2
A1
P3
84
2.0
0.0
P1
04
P2
lsw201 20404249_R8K
A1
P3
C4
0.0
0.0
P2
P1
E4
A1
lsw201 20406578_Ex1
P3
04
2.0
0.0
P2
A1 A1
P1
F4
lsw201 20406578_R8K
P3
E4
0.0
0.0
P2
P1
G4
lsw201 20406998 _Ex1
P3
F4
2.0
0.0
P2
P1
A1
H4
lsw201 20406998_R8K
P3
G4
0.0
0.0
P1
85
P2
lsw201 20407000_R8K
A1
P3
H4
0.0
0.0
P1
C5
P2
lsw201 20406771 _Ex1 .2sp
A1
P3
A5
2.0
0.0
P2
A1
P1
05
lsw201 20406771 _Ex1 .2epi
P3
85
2.0
0.0
P1
P2
E5
lsw201 20406771 _R8Qsp
A1
P3
C5
0.0
0.0
P2
A1
P1
F5
lsw201 20406771 _R8Qepi
P3
05
0.0
P1
0.0
G5
edmh_201 204071 1 1 _1
P2
A1
P3
E5
0.0
0.0
P1
P2
H5
edmh_201 204071 1 1 _2
A1
P3
F5
0.0
0.0
P1
P2
A6
A1
edmh_201 204071 1 1 _3
P3
G5
0.0
0.0
P2
A1
P1
86
edmh_201 204071 1 1 _4
P3
H5
0.0
2.3
P1
C6
P2
edmh_201 204071 1 1 _R8Q
A1
P3
A6
0.0
0.0
P2
A1
P1
06
edmh_201 204071 1 3_1
P3
86
0.0
0.0
P1
E6
edmh_201 204071 1 3_2
P2
A1
P3
C6
0.0
0.0
P1
P2
F6
edmh_201 204071 1 3_R8Q
A1
P3
06
0.0
0.0
P1
G6
edmh_201 2040271 3_ 4
P2
P3
A1
E6
4.5
0.0
P2
A1
P1
H6
edmh_201 2040271 3_RBQ2
P3
F6
0.0
0.0
P1
P2
A7
edmh_201 30400029_1
A1
P3
G6
2.0
0.0
P2
A1
3.4
00 .
P2
0.0
0.0
P2
H6
A1
P1
87
edmh_201 30400029_2
P3
P1
C7
edmh_201 30400029_3
P3
A7
P1
07
edmh_201 30400029_4
P3
87
2.6
P1
0.0
E7
P2
edmh_201 30400029_RBQ
A1
P3
C7
0.0
0.0
P2
A1
A1
P1
F7
edmh_201 204071 1 1 _5_FTA
P3
07
0.0
G7
7.1
P1
P2
edmh_20120407111_RBQ_FTA
A1
P3
E7
0.0
0.0
P2
A1
P1
H7
edmh_201 204071 1 1 _6
P3
F7
2.0
0.0
P1
AS
P2
edmh_201 204071 1 1 _RBK
A1
P3
G7
0.0
0.0
P2
A
D'?f1
AR �n l!ln1�
AR 30Jan1 3
flV?(
v
...
-
Ext Pit
Source
Sample Name
Well
P1
88
-- - --
edmh_201 204071 1 3_3
-
---
-
·
-
·
--
Norm Pit
New Well
P3
H7
-·-
-
-
Template vol (ul) 2.0
TE_vol 0.0
TE Reservoir P2
TEwell A1
P1
CB
edmh_201 204071 1 3_4
P3
AB
2.7
0.0
P2
A1
P1
DB
edmh_201 204071 1 3_5
P3
88
2.0
0.0
P2
A1
P1
E8
edmh_201 204071 1 3_RBK
P3
P1
F8
lsw201 30400493_Ex2
P3
CB
08
0.0
0.0
P2
A1
0.0
0.0
P2
A1
P1
GB
lsw201 30400493_RBQ
P3
E8
0.0
0.0
P2
A1
P1
A1 2
APC_AR30Jan 1 3
P3
F8
6.0
0.0
P2
A1
ANC_AR30J an 1 3
P1
81 2
P1
empty
P1
A9
P1
89
P3
GB
0.0
0.0
P2
A1
P3
H8
0.0
0.0
P2
A1
jtm201 20404427_2_mn2
P3
A9
0.0
0.0
P2
A1
jtm201 20404427_RBQ_mn2
P3
89
0.0
0.0
P2
A1
P?n AR �n.l::1�
AR 30Jan1 3
�
Ext Pit
-
·
-
- · --
------ - �
Sample Name
Well
-
-
--
·
-
-
--
Norm Pit
New Well
· Template-
Reservoir
-
---
TE well A1
P2
20.0
P2
A1
45.5
P2
A1
1 6.3
P2
0.0
P2
A1
A1
P1
81
1sw201 20406772_R8Q
P3
81
P1
C1
lsw201 2040680 1 _Ex1
P3
C1
0.0
P1
01
lsw201 20406801 _Ex2
P3
01
0.0
P1
E1
lsw201 20406801 _Ex5
P3
E1
20.0
P1
F1
lsw201 20406801 _Ex6
P3
F1
20.0
P1
G1
lsw201 20406801 _Ex7
P3
G1
P1
H1
A2
lsw201 20406801 _Ex8
P3
H1
tsw201 20406801 _R8Q
P3
82
lsw201 20406045_Ex1
P3
P1
C2
lsw201 20406045_R8Q
P3
P1
E2
lsw201 1 0404 779_Ex9.2
P1
F2
lsw201 1 0404779_R8Q
P1
G2
lsw20090401 38 4_Ex2.3
P1
G2
lsw20090401 38 4_Ex2.3
P3
...- P1
H2
lsw20090401 38 4_R8K-3
P3
P1
A3
lsw201 20406645_Ex6
P3
H2
P1
A3
lsw201 20406645_Ex6
P3
P1
83
lsw201 20406645_RBK
P1
C3
P1
-
-
·
A1
0.0
P2
A1
0.0
P2
A1
0.0
P2
A1
20.0
0.0
P2
A1
1 5.5
0.0
P2
A1
20.0 20.0
82
-
0.0
P3
P1
TE
0.0
lsw201 20406772_Ex3
A2
-
TE _vol
20.0
A1
·
-
vol (ul)
P1
P1
..
. - -
-
Source
-
C2
20.0
0.0
02
0.0
P2 P2
A1
12 0
E2
20.0
0.0
P2
A1
0.0
98.9
P2
A1
F2
0.0
98.9
P2
A1
G2
20.0
0.0
P2
A1
0.0
63.0
P2
A1
H2
0.0
63.0
P2
A1
P3
A3
20.0
0.0
P2
A1
lsw201 20406801 _ Ex3
P3
83
0.0
52.4
P2
A1
03
lsw201 20406801 _R8K-FTA
P3
C3
20.0
0.0
P2
A1
P1
E3
lsw201 20406801 _Ex4
P3
03
0.0
1 07.9
P2
A1
P1
F3
lsw201 20406801 _RBK
20.0
0.0
P2
A1
0.0
8 7.2
P2
A1
P2
A1
P2
A1
P2
A1
G3
P1
P3
P3 P3
.
F2
P3
E3
lsw201 20406045_Ex2
P3
F3
A1
P1
G3
lsw201 20406045_Ex2
P3
F3
0.0
87.2
P1
H3
lsw201 20406045_RBK
P3
G3
20.0
0.0
P1
A4
lsw201 20406771 _Ex1 .1
P3
H3
0.0
88.4
P1
A4
lsw201 20406771 _Ex1 .1
P3
H3
0.0
88.4
P2
A1
P1
84
lsw201 20406771 _RBK
P3
A4
20.0
0.0
P2
A1
P1
C4
lsw201 20404249_Ex5
P3
84
0.0
70.1
P2
A1
P1
C4
lsw201 20404249_Ex5
P2
A1
P1
04
, P1
84
0.0
P3
C4
20.0
0.0
P2
A1
E4
lsw 201 20404249_RBK
P3
70.1
lsw201 20406578_Ex1
P3
04
0.0
99.0
P2
A1
P1
E4
lsw201 20406578_Ex1
P3
04
0.0
99.0
P2
A1
P1
F4
lsw201 20406578 _RBK
P3
E4
20.0
0.0
P2
A1
P1
G4
lsw201 20406998_Ex1
P3
F4
0.0
1 00.0
P2
A1
P1
G4
lsw201 20406998_Ex1
P3
F4
0.0
1 00.0
P2
A1
P1
H4
lsw201 20406998 _RBK
P3
G4
20.0
0.0
P2
A1
P1
85
lsw201 20407000_RBK
P3
H4
20.0
0.0
P2
A1
P1
C5
lsw201 20406771 _Ex1 .2sp
P3
0.0
79.4
P2
A1
P1
C5
lsw201 20406771 _Ex1 .2sp
P3
0.0
P2
A1
P1
05
lsw201 20406771 _Ex1 .2epi
P3
0.0
79.4 81 .7
P2
A1
P1
05
lsw201 20406771 _Ex1 .2epi
P3
85
0.0
81 .7
P2
A1
P1
E5
lsw201 20406771 _RBQsp
P3
C5
20.0
0.0
P2
A1
P1
F5
lsw201 20406771 _RBQepi
P3
05
20.0
0.0
P2
A1
P1
G5
edmh_201 204071 1 1 _1
P:3
E5
20.0
0.0
P2
A1
P1
H5
edmh_201 204071 1 1 _2
P3
0.0
P2
A1
P1
A6
edmh_201 204071 1 1 _3
P-3
F5 G5
20.0 20.0
0.0
P2
A1
P1
86
edmh_201 204071 1 1 _ 4
. P3
H5
1 7.7
0.0
P2
A1
P1
C6
edmh_201 204071 1 1_RBQ
P3
A6
20.0
0.0
P2
A1
P1
06
edmh_201 204071 1 3_1
P3
P1
E6
edmh_201 204071 1 3_2
P1
F6
edmh_201 204071 1 3_R8Q
P1
G6
edmh_201 2040271 3_4
P1
H6
edmh_201 2040271 3_RBQ 2
n.-,nn
AD ':U11-:n1'<
AS AS
85
86
20.0
0.0
P2
A1
C6
20.0
0.0
P2
A1
P3
06
20.0
0.0
P2
A1
P3
E6
0.0
1 5.5
P2
A1
P3
F6
20.0
0.0
P2
A
P3
AR 30Jan1 3
iJl
v
·
· -----
Ext Pit
Source Well
Sample Name
-
-·
-
- -
-
Norm Pit
New Welt
----
-
----
Template
)
vel {ul
-
-
TE_vot
-
--
-
-
TE Reservoir
-
- -
· - - --
TEwell
P1
A7
edmh_201 30400029_1
P3
G6
0.0
75.1
P2
A1
P1
87
edmh_201 30400029_2
P3
H6
0.0
1 6.6
P2
A1
P1
C7
edmh_201 30400029_3
P3
A7
20.0
0.0
P2
A1
P1
07
edmh_201 30400029_4
P3
87
0.0
1 7.4
P2
A1
P1
E7
edmh_201 30400029_R8Q
P3
C7
20.0
0.0
P2
A1
P1
F7 G7
edmh_201 204071 1 1 _5_FTA
P3
0.0
edmh_20120407111_RBQ_FTA
P3
07 E7
1 2.9
P1
20.0
0.0
P2
P2
A1 A1
P1
H7
edmh_201 204071 1 1 _6
P3
F7
0.0
77.2
P2
A1
P1
AS
edmh_201 204071 1 1 _RBK
P3
G7
20.0
0.0
P2
A1
P1
88
edmh_201 204071 1 3_3
P3
H7
0.0
59.6
P2
A1
P1
C8
edmh_201 204071 1 3_4
P3
AS
0.0
1 7.3
P2
A1 A1
P1
08
edmh_201 204071 1 3_5
P3
88
0.0
60.8
P2
P1
08
edmh_201 204071 1 3_5
P3
88
0.0
60.8
P2
A1
P1
E8
edmh_201 204071 1 3_R8K
P3
C8
20.0
0.0
P2
A1
P1
F8
lsw201 30400493_Ex2
P3
08
20.0
0.0
P2
A1
P1
G8
lsw201 30400493_RBQ
P3
EB
20.0
0.0
P2
A1
0.0
1 4.0
P2
A1
0.0
P2
A1
P1
A1 2
APC_AR30Jan1 3
P3
F8
P1
81 2
ANC_AR30Jan1 3
P3
GB
20.0 .
P1
empty
P3
H8
0.0
0.0
P2
A1
P1
A9
jtm201 20404427_2_mn2
P3
A9
·0.0
0.0
P2
A1
P1
89
jtm201 20404427_RBQ_mn2
P3
89
0.0
0.0
P2
A1
yJ-(f
AR �n.l�n1�
- --
-
--
·
Florida Department of Law Enforcement
(FDLE
Biology" Section
3130x/ Plate Record Biomek 2000 Operator: Analyst(s): Date: Plate Record Name: Biomek 2000 Method:
Instrument ID: Capillary Array Lot: Array Count or Date: POP4 Lot: Buffer Lot:
�io·s� �W�if� A1 A2 81 82 C1 C2
01
02 E1 E2 F1 F2 G1 G2 H1 H2
A3 A4 83 84 C3 C4 03 04 E3 E4 F3 F4 G3 G4 H3 H4
A5 AS 85 66 C5 05 06 E5
E6 F5 F6 G5 G6 H5 HS
../=Used V- "'1-' I
1--..J
10+ 10+ 10+ 10+ 10+ 10+
10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+
10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+
10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+ 10+
3130xiB 32A06704 7 1209121 1209410
10+ Ladder lsw20120406772 Ex3 lsw20120406772 RBO lsw20120406801 Ex1 lsw20120406801 Ex2 lsw20120406801 Ex5 lsw20120406801 Ex6 lsw20120406801 Ex7 lsw20120406801 Ex8 lsw20120406801 RBQ lsw20120406045 Ex1 lsw20120406045 RBQ lsw20110404779 Ex9 .2 lsw20110404779 RBQ lsw20090401384 Ex2.3 lsw20090401384 RBK-3 10+ Ladder lsw20120406645 Ex6 lsw20120406645 RBK lsw20120406801 Ex3 lsw20120406801 RBK-FTA lsw20120406801 Ex4 lsw20120406801 RBK lsw20120406045 Ex2 lsw20120406045 RBK lsw20120406771 Ex1.1 lsw20120406771 RBK lsw20120404249 ExS lsW20120404249 RBK lsw20120406578 Ex1 lsw20120406578 RBK lsw20120406998 Ex1 10+ Ladder lsw20120406998 RBK lsw20120407000 RBK lsw20120406771 lsw20120406771 Ex1.2epi lsw20120406771 lsw20120406771 RBQepi edmh 20120407111 1 edmh 20120407111 2 edmh 20120407111 3 edmh 20120407111 4 edmh 20120407111 RBQ edmh 20120407113 1 edmh 20120407113 2 edmh 20120407113 RBQ edmh 20120402713 4
Pace 1
rf
lsw edmh
. };_,· :�---�--
31-Jan-13
AR 30Jan13 61-75 ID+ Ladder Lot:
1205041
Yfiler Ladder Lot: Formamide Lot:
GS 600 Lot: Denature:
1202160 1207049 TC#3@ 95d
!?
A7 AS 87 88 C7 CB 07 DB E7 EB F7 FB G7 GB H7 HB A9 A10 89 810 C9 C10 09 010 E9 E10 F9 F10 G9 G10 H9 H10 A11 A12 811 812 C11 C12 011 012 E11 E12 F11 F12 G11 G12 H11 H12
./=Used
ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ ID+ 10+ 10+ ID+ 10+ 10+
ID+ Ladder edmh 20120402713 RBQ2 edmh 20130400029 1 edmh 20130400029 2 edmh 20130400029 3 edmh 20130400029 4 edmh 20130400029 RBQ edmh 20120407111 5 FTA edmh 20120407111 RBQ FTA edmh 20120407111 6 edmh 20120407111 RBK edmh 20120407113 3 edmh 20120407113 4 edmh 20120407113 5 edmh 20120407113 RBK lsw20130400493 Ex2 10+ 10+ Ladder ID+ lsw20130400493 RBQ 10+ APC AR30Jan13 ID+ ANC AR30Jan13 10+ no 10+ 2 mn2 10+ RBQ mn2 10+ no 10+ no sample 10+ no sample 10+ no 10+ no sample 10+ no sample 10+ no sample 10+ no sample 10+ no sample
ID+ 10+ 10+ 10+ 10+ ID+ 10+ 10+ 10+ 10+ 10+ ID+ ID+ 10+ 10+ ID+
Paae 2
plexor plexor
Florida Department of Law Enforcement Biology Section RUN ANALYSIS FORM Analyst
fW.
Run Folder.
Case Number(s) or Sample Set
•
S
!r.:o:> Location:
!-'\.
�
t.SN
GS600 present and correc tly labeled
•
All ladders have correct allelic calls
•
Alleles above threshold/below
•
All controls have correct results (Positive/Negative/Blanks)
max rfu,
peak heights within ratio
Comments:
� 0 \� bL-\ 6 �OL\S
\
�
Date
Original in first case listed
Applied B1osystems
31308 AR 30Jan131sw
GeneMapper10 v3.2.1
Name
File
A02 002 lsw20120406772 Ex3 90
150
Panel
Ex3
0 1.fsa
270
210
Plus Panels v1
0 330
390
90 60
. A02 路ooz lsw20120406772 Ex3 90
Ex3
o 1.fsa 150
210
0
Plus Panels v1
.
270
.
330
390
90 60
A02 002 lsw20120406772 Ex3 90
0 1.fsa 150
lsw20120406772 Ex3 210
0 270
ldentifiler Plus Panels v1 330
390
90 60 30
Ao2 002 lsw20120406772 Ex3 90
801 003 lsw20120406772 RBQ 90
0 1.fsa 150
lsw20120406772 Ex3 210
150
270
RBQ
0 1.fsa
0
210
ldentifiler Plus Panels v1 330
0 270
390
Plus Panels v1 330
390
90 60 30
Mon Feb 11,2013 01:57PM, EST
Printed by: lsw
Page 1 of 2
Applied B1osystems
31308 AR 30Jan13 lsw
GeneMapper ID v3.2.1
Name
File
801 003 lsw20120406772 RBQ
0 1.fsa 150
90
Panel
lsw20120406772 RBQ 210
0 270
ldentifiler Plus Panels v1 330
390
90 60
801 003 lsw20120406772 RBQ 路o 1.fsa 150
90
lsw20120406772 RBQ 210
0 270
ldentifiler Plus Panels v1 330
390
90 60 30
801 003 lsw20120406772 RBQ 90
o
1.fsa
lsw20120406772 RBQ
150
210
0 270
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Page 1 of 1
·FDLE
Florida Department of
Jacksonville Regional Operations Center
Rick Scott, GJvernor
Law Enforcement
921 N. Davis Street, Building E Jacksonville, Florida 32209-683 8
Pam Bond� Attorney General
Gerald M. Bailey
1-800-226-6481
Adam Putnam, Commissioner of Agriculture
Commissioner
www.fdle.s tate.fl.us
Jeff Atwater, Chief Financial Officer
LABORATORY REPORT February12,2013 Sheriff John H. Rutherford
TO:
FDLE NUMBER:
Jacksonville Sheriffs Office
SUBMISSION:
501 East Bay Street
AGENCY NUMBE R:
Jacksonville, FL 32202-2975 ATTN:
M. A. Musser61139
VICTIM (S):
Jordan Russell Davis
SUBJE CT(S):
Michael David Dunn
0 FFENSE(S):
20120406772 1 and2
12-0853093
SUBPOENAS PERTAINING TO THIS CASE SHOULD REFE R TO THE FDLE NUMBER.
Death Investigation
L. SukHan Warf
Duval County 11/23/2012
Crime Laboratory Analyst Biology Section
RE FE RENCE :
This report references evidence submited to the Florida Department of Law Enforcement on December06,
2012 and on December18,2012 by Maurice Lawrence. This report may contain conclusions, opinions, and/ or interpretations made by the author. E VIDENCE : FDLE
Agency
Item#
E xhibit# 1-9
3 8
9 10
6-2 6-3 6-4
Description
DNA swab from shell casings at scene
DNA swabs from shell casings recovered from suspect vehicle DNA swab from top of magazine recovered from suspect vehicle DNA swab from live rounds recovered from suspect vehicle
RE SULTS:
STR DNA analysis was performed on a sample from Exhibit 3 utilizing the AmpF lSTR Identif.tler Plus PCR Amplification Kit.
No DNA results were obtained from Exhibit 3 . STR DNA analysis was not performed on Exhibits 8,9 and10 .
1111111111111111111111111111111111111111
Service
Page 1 of2
•
Integrity
•
Respect
•
Quality
FDLE NUMBER: 20120406772 Submissions: 1 and 2 REMARKS: ·
·· · · ·
The·submited evidence· is available-Jor retrieval orreturatthe earliest opportunity; Thls evidence ·includes DNA extracts. It is recommended that all DNA extracts be stored frozen. Abbreviations that may appear in this report are: CODIS Combined DNA Index System; DNA
deoxy ri bonuc leic acid; PCRPolymerase Chain Reac tion; STR short tandem repeat; Y STR male spe cific short tandem repeat; FBI F e de ral Bureau ofInvestigation; and JFS Journal ofForensi c Sciences. -
Questions regarding this report should be addressed to: jrocbiology@fdle. state.fl.us.
Page� of2
ASCLDILAB-International
STATEMENT OF QUALIFICATIONS
Name
Date
Lucretia SukHan Warf
of Law Enforcement I Jacksonville
Florida
Job Title
sno12o13 Center
Crime
Indicate all disciplines in which you do casework:
D D D D D
Drug Chemistry Firearms/Tool marks Trace Evidence Latent Prints
Digital & Multimedia Evidence
D IX] D D
Toxicology Biology Questioned Documents Crime Scene
L ist all category(ies) of testing in which you do casework:
and DNA. Breath Alcohol Calibration Categories
D
D
Toxicology- Breath Alcohol Measuring Instruments (The work of the laboratory MUST include calibration certificatesdo not check the box if work is limited to breath/alcohol testing)
- Breath Alcohol Calibration Reference Material
Education: List all higher academic institutions attended (list high school only if no college degree has been attained) Institution
Dates Attended
ofNorth Florida
8/1998 - 5/2002
of Florida
5/2003 - 1 2/2005
Major
Degree
B. S.
Forensic DNA & Serology
M.S.
Other Training: List continuing education, workshops, in-service and other formal training received. Please include the course title, source and date of the training.
University of North Florida, Biochemisty FDLE Forensic Technologist Serology and DNA training program FDLE Crime Laboratory Analyst Serology and DNA training program Paternity Index DNA Statistics, SAFS, MAFS, MAAFS, CAS Joint meeting Applied Biosystems GeneMapper ID Workshop, SAFS, MAFS, MAAFS, CAS Joint meeting GeneMapper ID Training, FDLE 3130xl Genetic Analyzer training, FDLE Statistics in the Courtroom, 5th Annual Advanced DNA Technology Workshop, The Bode Technology Group, Inc. Beckman Biomek 3000 and 2000 rpbotics training with Promega's DNA IQ extraction system, FDLE HID University 3130xl and Genemapper ID training, Applied Biosystems FBI DNA Auditor Workshop DNA Identifications for Missing Persons and Disasters SAFS, MAFS, MAAFS, SWAFS Joint meeting YSTRs: Science and Statistics SAFS, MAFS, MAAFS, SWAFS Joint meeting ASCLD/LAB International Preparation Course, ASCLD/LAB Plexor HY
and PowerPlex Y training, Promega
ASCLD/LAB-lnternational Statement of Qualifications
Approval Date: August 3, 2012 Approved By: Executive Director
Page 1 of2
Effective Date: August 3, 2012 AL-PD-3018-Ver 3.0
Plexor HY quantitation training, FDLE
Yfiler training, FDLE
Identifiler Plus training, FDLE Courtroom Experience: List the discipline/category(ies) of testing in which you have qualified to testify and indicate over what period of time and approximately how many times you have testified in each.
as
an expert witness
Serology (since 2005):25 DNA (since 2005) :35 Professional Affiliations: List any professional organizations of which you are or have been a member. Indicate any offices or other positions held and the date(s) of these activities.
Employment History: List all scientific or technical positions held, particularly those related to forensic science. List current position first. Be sure to indicate employer and give a brief summary of principal duties and tenure in each position.
Job Title
Tenure
Crime
2/2005 -
FDLE Provide a brief
Job Title
duties:
of
STR-DNA
evidence
of evidence and courtroom
Tenure
Crime
Crime
FDLE of
Provide a brief In
duties : of evidence and courtroom
to learn STR-DNA
Job Title
Tenure
Forensic
8/2002 - 5/2004
FDLE of
Provide a brief
duties :
General lab support, instrument maintenance and operation, analyst support, sweep cases for trace evidence, general serology evidence
Job Title
Tenure
Volunteer
3/2002 - 8/2002
FDLE duties:
Provide a brief description of Evidence
office
and
Tenure
Job Title Provide a brief
of
duties :
Other Qualifications: List below any scientific publication and/or presentation you have authored o r co-authored, research in which you are or have been involved, academic or other teaching positions you have held, and any other information which you consider relevant to your qualification as a forensic scientist. (Use additional sheets if necessary. )
FDLE Plexor HY and Y-filer internal validation team
ASCLD/LAB-lnternational Statement of Qualifications
Approval Date: August 3 , 20 1 2
Approved By: Executive D irector
Page 2 of 2
Effec-tive Date: August 3, 20 1 2 AL-PD-3 0 1 8-Ver 3 .0
tj_
Form
Full Member
IF Associate Member, Name of Full Member Sponsor
Badge Name
tY'-:C. \ ·L-£.
Drivers License No . Newsletter by Email? Email Address :
.
.;:' ) Yes
4- -1
D 6 <;
.J
-4 ... Vice President.
No
I
NRA Membership Number
�- Cbc"'
.
Treasurer
Executive Officer
Sign me up for the NRA
I wil email my NRA number
Initiation F ee Yearly Dues NRA Fee Total
I certify that I
1, () 6 �s
?>�1
of good repute; that I am not a member of any organization or group having as its purpose the overthrow or
disruption by force or viol ence the Gov ernment of the United States of America or any of the poli tical subdivisions; that I have am
never been convicted of a crime of violence; that if admitted to membership, I will fulfill the obligations of good sportsmanship and good citizenship . I hereby attest the above statements are true, and any falsehood will deprive me of membership. I understand that I must remain an NRA member for the duration of my club membership. I also understand that by signing this application I am consenting to a possible background check.
Applicant Signature
FOR CLUB USE ONLY Orientation Attended:
�
Date Orientation Lead Signature
Page �
Revised 1 0/2 1 109
RELEASE AND HOLD HARMLESS AGREEMENT In consideration for participating in club activity sponsored or organized by the Port Malabar Rifle and Pistol Clu b, Inc., the undersigned Participant HEREBY AGREES TO WAIVE, RELEASE, DISCHARGE, AND HOLD HARMLESS the Port Malabar Rifle and Pistol Club, Inc . from any and all claims for damages for death , personal injury, or property damage which they may have or which they may hereafter accrue as a result of participation in any Port Malab ar Rifle and Pistol Club, Inc. program or related activity and events including, but not limited to unorganized practice, organized practice, training and instruction and organized shooting events. By signing this Release and Hold Harmless Agreement, the undersigned recognizes that the sport of rifle, pistol, and shotgun shooting or related activities present certain inherent dangers that cannot be eliminated even by the exercise of reasonable care. As such, the undersigned Participant hereby expressly assumes the risk that is inherent to the sport of rifle, pistol, and shotgun shooting. As a requisite condition to the Participant being allowed to attend and participate in any Port Malabar Rifle and Pistol Club, Inc. sponsored event or trip, the undersigned Participant hereby acknowledges that the State of F lorida has the most significant relationship with the p arties to this Agreement rather then the destination state or providence. As such, the parties hereto expressly agree to F lorida
as
the Choice of Law and Choice of Forum to any claim, suit, or controversy that m ay arise by and between the
p arties or any of its related affiliates, and further agree that any action, suit, or claim made by the Particip ant
must
be brought in the
State of Florida as the proper Venue having exclusive subj ect matter jurisdiction to -any such claim, demand, or suit. To effectuate this expressed Choice of Law, Choice of Forum and V enue provision, the undersigned hereby agrees that the state or providence where the injury or cause of action arose shall hereto .
not have
either subj ect matter jurisdiction or
in personam jurisdiction
over the p arties
By signing this Release and Hold Harmless Agreement, the undersigned Participant assumes the risk inherent to rifle, pistol, and shotgun shooting, and thereby consents to the Participant's p articipation in Port Malabar Rifle and Pistol Club, Inc. programs and related activities, and further acknowledge that he/she understands that all risks, whether known or unknown, are expressly assumed by the undersigned, and that all claims, whether known or unknown, are expressly waived in advance. In addition to the foregoing, the undersigned Particip ant hereby expressly agrees and recognizes that the Port Malabar Rifle and Pistol Club, Inc., through its individual Match Directors, Match Chairmen, Range Officers, judges and referees, officers, directors, agents, individuals or entities perform
volunteer services,
and as such, said entities and/or individuals are immune from
liability as volunteer leaders, assistants, and organizers.
Arbitration :
I n further consideration for being allowed t o participate i n any activities sponsored b y the Port Malabar Rifle and Pistol Club, Inc. the undersigned P articipant recognizes and agrees that this contract involves interstate commerce which is necessarily
ยง 2), and therefore, pursuant to the Federal "Arbitration Act (FAA) as well as (Florida Statutes ยง 682.02) hereby agree to submit to binding arbitration any and all claims, demands,
controlled by the Federal Arbitration Act (9 USC the Florida Arbitration Act
. suits, or other disputes that may arise out of this contract, or that may be presented by or on beh alf of the Participant for resolution
under the Commercial Rules of the American Arbitration Association with the initial filing fees thereof to be paid by the party filing the Demand for Arbitration subject to the schedule of fees then in effect at the time of the filing of a Demand with the American Arbitration Association. I HAVE READ THE FOREGOING TERMS AND CONDITIONS AND AGREE TO BE BOUND BY ALL OF THE CONDITIO
S S ET FORTH ABOVE.
Page 1
Revised 1 0/2 1/09
Detective Detective Detective Detective
T. A. Stapp #7725 M . A. M usser #61 1 39 (Lead Detective) E. M. Cayenne #6060 T. D. O l iver #7779
WITNESSES INTERVIEWED BY DETECTIVE CAYENNE
D ETAILS OF I NVESTIGATION F riday/1 1 -23-1 2/2000 hours : Detective Cayenne received a phone call from Sergeant Paul advising our team was bei n g called to 8251 Southside Boulevard (Gate Gas Station) in reference to a shooting in the parking lot. Sergeant Pau l advised that when we are done with our other crime scene we could meet at the Gate Gas Statio n . Note: Let i t be n oted that Detectives Stapp and Cayenne was handling another death investigation a n d never made it to the crime scene. F riday/1 1 -23-1 2/231 0 hours: Detective Cayenne spoke with Sergeant Paul and advised him that Detectives Cayenne and Sta pp were done with our death i nvestigation where did he need us to respond. Sergeant Pau l advised Detective Cayenne to respond to t h e Homicide Office. F riday/1 1 -23-1 2/230 hou rs: Detective Cayenne arrived at the Homicide Office. Tuesday/1 1 -27 -1 2/1 805 hours : Detectives Caye n ne and Stapp responded to the strip mall located at 8221 Southside Boulevard to cond uct a canvass of the businesses. Detective Cayenne spoke with owners or employees of the following businesses: U n it 3- Kalu by's Dance Club advised they had video surveillance i nside the business and outside on the back side. There was no video outside the front of the business. U n it 5 - The Loop Restaurant advised the business has s u rveillance inside and views outside the rear of the business. There was no video outside the front of the business. U n it 1 1 - Tropical Smoothie advised the business has surveillance inside and views outside the rear of the business. There was no video outside the front of the business. Detective Cayenne spoke with employee who stated he was working on the n i g ht of the shooting. He advised he d id not hear any g u nshots. remem bered a police car had pulled over a veh icle in the Publix parking lot. The next th ing he remem bered were m u ltiple police cars responding to the scene. He did not learn of the shooting until later that night. Detective Cayenne spoke with witness the incident. did not hea r any g u nshots.
SUP
Page 2 of 4
Date Printed: 1 013/2013
who was working on the night of
J O H N H . RUTH E RF O RD, S H E RI F F
2012 853093-9
SUP
Case Cleared CLEARED
BY ARREST (DETECTIVE)
CRIME ANAL YSIS Aggravated Assault/Murder: ARG U M ENT Type of Weapon: HAN DGUN ( DISCHARGED) Forced Entry: NOT APPLICABLE Structure Occupancy Code: NOT APPLICABLE Number ofPremises Entered: Location Type: Gas/Service Station(business that services vehicles) Incident Occurred Outside th is Location Incident Occurred In the Parking Lot at this Location: Number ofArrested: 1 Number of Vehicles Recovered: School Name: School Number
Yes
MISCELLANEOUS: Is Offense Related to Domestic Violence: No Ifyes, were Children under 18 Present: No IfNo is it Domestic Related: NO Is there additional information included on a continuation report: No Are there other Pertinent Reports: Yes In your opinion is there sign(ficant reason to believe that the crime can be solved by a patrolfollow-up investigation? No Neighborhood Canvass Conducted: Yes Case Information Card Left With: N.A. If Other (Name Address): Address: Apt./Lot #: Sub-Sector: City: State: Zip: Taz: Crossstreet: Home Phone # Bus. Phone # Ext. Cell Phone # Cell Phone Provider E-mail Is Vagrant?
NO
Investigative Time: #1 0 Hours: 1 0 Minutes: 0
Cost Amount:
Detective Called To the Scene: M .A . M USSER
Evidence Technician Called to the Scene: Reporting Officers:
SUP
#61 1 39
#0
E.M.CAYEN N E
Approving Supervisor M .A. PAU L Division: DETECTIVE Unit:
Page 4 of 4
$482.40
( 1 [ 1 ID #6060 1D #0 ID #6097
NA:
Not(fied: [ X
NA:
Assigned By HQ: [ X
1
8/1 5/201 3
1 1 3 :05
HOMICIDE
Date Printed: 1 01312013
J O H N H . RUTH E RF O R D , S H E RI F F
2012 853093-9
SUP
at the B u rger King located near the i ntersection of Beach Boulevard and St. Johns Bluff. Th is report documents all ind ivid ual investigative efforts in this case that were conducted by Detective Oliver. Refer to Detective M usser's report for more detai ls of the investigation.
Clearance Status: CLEARED BY ARREST Clearance Code: Case Cleared C LEARED BY ARREST (DETECTIVE)
ADULT Date case was cleared: 1 1 /24/201 2
Number ofCases Cleared
CRIME ANAL YSIS Aggravated Assault/Murder: ARG U MENT Type of Weapon: HANDGUN (DISCHARGED) Forced Entry: NOT APPLICABLE Structure Occupancy Code: NOT APPLICABLE Number ofPremises Entered: Location Type: Gas/Service Station(business that services veh icles) Incident Occurred Outside th is Location Incident Occurred In the Parhng Lot at this Location: Number of Vehicles Recovered: Number ofArrested: 1 School Name: School Number
Yes
MISCELLANEOUS: Is Offense Related to Domestic Violence: No Jfyes, were Children under 18 Present: No IfNo is it Domestic Related: NO Is there additional information included on a continuation report: No Are there other Pertinent Reports: Yes In your opinion is there significant reason to believe that the crime can be solved by a patrolfollow-up investigation? No Case Information Card Left With: N .A. Neighborhood Canvass Conducted: Yes If Other (Name Address): Address: Apt./Lot #: Sub-Sector: City: State: Zip: Taz: Crossstreet: Home Phone # Bus. Phone # Ext. Cell Phone # Cell Phone Provider E-mail Is Vagrant?
NO
Investigative Time: #1 1 Hours: 0 Minutes: 0
Cost Amount:
Detective Called To the Scene: M .A.M USSER Evidence Technician Called to the Scene: Reporting Officers:
T. D. 0 LIVER
Approving Supervisor M .A. PAUL Division: DETECTIVE Unit:
SUP
Page 3 of 3
#61 1 39
#0
NA:
[ 1 1 #7779 #0 #6097
NA: [ ID ID ID
Notified:
[X1
AssignedBy HQ: [
X1
8/1 9/201 3
1 4:23
HOMICIDE
Date Printed: 1 0/312013
J O H N H . RUTH E RF O RD , S H E RI F F
2012 853093 1 0
SUP
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enough filler, on to the real news. Thought of you yesterday after all of this and said man Mike probably really enj oy this
article and the talk back section.
e Are Watching You Act, propo sed by
Democratic Massachuset Rep . Michael Capuano and
Rep lican North Carolina Rep. Walter Jones, will keep corporations from using digiW vide<>.t" recor ers (DVRs) to collect and share your personal information.
Kine info
Polygon reports. The bill would require the consumer to provide prior consent before
ation is collected, and the operator of the
data s collected, said a press release on the House's website. Infr
info offer
would have to provide access to how the
installed in the DVRs would be used to collect the surveillance ed cameras and would be forc�d to ation. If not given a consumer ' s permission to be filmed, the
other option that does not film their consumers but that is "otherwise identical in all aspects."
If a c nsumer does allow the information to be collected, a bright red message that reads "WE ARE WAT HING YOU" must be flashed across the screen at all times while recording. The
ouse 's press release explains the teci.Jx.• companies ' interest in this technology: Essentially, they
want to better and more quickly figure out what to sell you- a way to "super-target ads," Capuano said .
"If s meone is watching a favorite program while cleaning the kitchen, ads for products that will make appli ces shine may show up. If a couple is talking about where to dine over the weekend, or enjoying
ac
chy snack while watching the Stanley Cup finals, their television may serve up food suggestions.
The atent application even goes so far as to suggest that if a couple is fighting, an adverti semen.tt"'
asso iated with relationship counseling might
be selected for them," it said.
Jone and Capuano both offered quotes in their press release.
In li ht of the government's "unfortunate history of secretly collecting private citizens ' information we must ensure
from Jone said. Cap and
that safeguards are in place to protect Americans ' rights,"
o stressed that though "this may sound preposterous ," it is "neither a j oke nor
an
exaggeration,"
ged consumers to consider the impact this sort of information- sharing will have on their personal about what you do in the privacy of your own home and then think about how· you would feel
g that information with your cable company, their advertisers and your
ress release concluded with the message that this is · a matter of awareness , and having a choice iri
atter.
minimum, consumers should have the option of saying no to being watched. " d it interesting . At first I thought it was another over reach of govenunent then I looked at the
Dire TV DVR box and re-read the article.
I realized this wasn't written specifically for the Xbox One.
This was written and aimed at Cable companies with DVR's. I had already read the comments section
at
s point and most of the comments were along the lines of. . . . er solution, don't buy an,
an Xbox. There i s n o need for a law. "
this is so over the top"
I.
I
Then
e post I replied to . . .
"Micr soft and other c omp anie s will make it al-o r-none . Either you enable the earnerďż˝ to transmit back
to the orne office, or you don't use the camera If
t
for anything. I still don't see the need for a federal law.
don't want to be survei lled, don't buy an Xbox. Sony or Nintendo will come out with a
co mp titive product that is not so intrusive. Fre My I too
this until
l
market
win."
I looked at my cable box. What if cable companies decided to do thisl We're
very I mited in what cable companies you can use. Without any oversight and very little effort, the
cable �ompanies could work together and push that out. Most people will do without a gaming
conso�es, how many would actually say no to cable?
I
f
Some ne else rep lyi ng to me
You :must admit tho ugh, the princ iple is the exact same no matter how much you want the product.
1/ I
I
I
I
My Reply back
While I do agree the federal government should be very limited in its interference with
anything, this is not one of those cases. The government has a j ob of protecting your right to
privacy. It is one of the few things the government is supposed to do. I have no prob lem with
the government telling co rporations that they us.
can not punish us if we don't allow them to spy o n
The same guy replying back to n1e
The government has no such duty to protect your right of privacy in voluntary transactions I between private citizens/entities. You have no more right to an xbox without a camera than a I right to vegetables without genetically modified organis ms . Nor do yo u have a ri ght to cable.
And what if you did? Take it to the logical conclusion, what other freely produced/purchased items would you then have the right to force (by the State, legislature) producers to make
according to your whim? I don't like being spied on, so I won't buy the pro duct . That's where
the analysis stops. My reply back
It does when it is the one using said corporations to spy on the citizens. Cable companies are
also not entirely private entities. They are highly subsidized by big government. If they were
truly priv ate entities many non..; spying
ones would pop up as soon all the maj or ones started spying. Ev ery home would have a choice of 2 or more cable providers but that isn't the case for
most people.
I don't eat GMO's but would agree the government has no right to make companies label GMO products .
Fre e Market works just fine because non-GMO foods make sure it is written al over.
As a co nsumer you're left with many choices.
Cabl e companies are comp letely different. It's about the control of information. If cable
companies tomorrow could tell you that you either put in a camera or they stop pro v iding you
TV/Internet access, you're at a serious disadvantage in life. �th the way things are going, that isn't completely far-fetched.
In a truly free society we wouldn't need this, but that's not where we are in history.
His Final Reply back to me. exists through State action. So maybe it is a different analysis with cable. But that is one of a
very few industries where that is the case. (Thankfully) It
as an interesting back and forth. I think I would actually argue that even in a case like the Xbox
One, as a consumer you should have the option of buying that hardware without the spy cam on it. I thi at some point, a conversation has to take place about the true role of the constitution and the fed ral government. I'm one of those that believes the federal governments j ob is to protect the bill of Sometimes the few need to be protected from the masses when it comes to the bill of rights. If rig 8 0° o of the population decided tomorrow that they don't care about their right to privacy and every
co pany out there started to put a c amera, microphone, and internet connection on their devices then req ·red you to have those parts operational in order to use the device, what happens to the 20% of pe le that don't want their privacy stolen from them? I'm talking about a stove, a microwave, a
· gerator, your cable box, your AC unit. This seems far fetched for now, but 20-3 0 years from now, I
do 't know that it will be as far fetched. I completely understand that in a truly free republic, this
wo ld never happen. But that's not what we have. We have a society filled with idiots that don't care
ab t any of their rights and will give them up for the latest gadget and a government that is loving the r new abilities to spy on the people. I think people need to be cautious when it comes to the kind of
}
leg slation. As I pointed out earlier in my replies, I don't think foods that contain GMO's should have to e labeled. I'm more concerned about a person's right to privacy in their home.
Fe eral Nullification efforts mounting in states . . . . Im gine the scenario: A federal agent attempts to arrest someone for illegally selling a machine gun. Ins ead, the federal agent is arested - charged in a state court with the crime of enforcing federal gun la s . Farfetched? Not as much as you might think.
Th scenario would become conceivable if legislation passed by Missouri's Republican-led Legislature � is s gned into law by Democratic Gov. Jay Nixon. be
Missouri legislation is perhaps the most extreme example of a states ' rights movement that has spreading across the nation. States are increasingly adopting laws that purport to nullify federal
s -. setting up intentional legal conflicts, directing local police not to enforce federal laws and, in rar cases, even threatening criminal charges for federat agents who dare to do their j obs. An Associated Press analysis found that about four-fifths of the states now have enacted local laws that
dir ctly rej ect or ignore federal laws on m arij uana use, gun control, health insurance requirements and
standards for driver 's licenses. The trend began in Democratic leaning California with a 1 9 6 medical marijuana law and has proliferated lately in Republican strongholds like Kansas, where Go . Sam Brownback this spring became the frrst to sign a measure threatening felony charges against ide
fed ral agents who enforce certain firearms laws in his state. So
e states, such as Montana and Arizona, have said "no" to the feds again and again
es' rights measures on all four subjects examined by the AP
"n " caries any legal significance.
-
-
passing
despite questions about whether their
"It see ¡s that there has been an uptick in nullification efforts from both the left and the right," said a professor at the University of California at Los Angeles who
Adam consti
Yet '
law.
specializes in
e law is clear - the supremacy clause (of the U.S. C onstitution) says specifically that the
laws are supreme over contrary state laws, even if the state doesn't like those laws," Wm.k.ler
feder
added
over some tates from flouting those federal laws - sometimes successfully.
state ones hasn't stopped
20 states now have medical marijuana laws allowing people to use pot to treat chronic pain and
other 'lments - despite a federal law that still criminalizes marijuana distribution and possession.
C edin ground to the states, President B arack Obama's administration has mad e it known to federal
prose utors that it wasn't worth their time to target those people.
authorities have repe atedly delayed implementation of the 2005 Real ID Act,
law or
r
an
anti-terrorism
set stringent requirements for photo identification cards to be used to board commercial flights
federal buildings. The law has been stymied, in part, because about half the state legislatures
have pposed its implementation, according to the National Conference of State Legislatures .
enacted measures challenging Obama's 20 1 0 health care laws, many of which sp e ci tcally rej ect the provision mandating that most people have health insurance or face tax penaltie s b e gi ing in 20 1 4 . Abou 20 states have
After Montana passed a
2009 law decl aring that federal fireans regulations don't apply to guns made
pt in that state, e i ght other states have enacted similar laws.
and The feder
upreme Court ruled in
1 997 that local police could not be compelled to carry out provisions of a
gun control law. But some states are now attempting to take that a step further by asserting that
certa n fede ral laws can't even be enforced by federal authorities .
Kans as law makes it a felony fo r a federal agent to attempt to enforce l aws on guns made and own d in Kansas. A similar Wyoming law, passed in 20 1 0, made it a misdeme anor. The Missouri bill
A ne
ould declare it a misdemeanor crime but would apply more broadly to all federal gun laws and reg ations - past, present, or future - that "infringe on the people's ri ght to keep and bear arms."
also
U. S .
General Eric Ho lder
sent a letter in late April to the Kansas governor warning that the al government is willing to go to court over the nevv lav.'. " Kansas may not prevent federal
emp oyees and officials from caring out their official responsibilities," Holder wrote. So "
woulďż˝ happen if a local pro sec utor actually charges a federal agent for doing his or her job?
Th y're going to have problems if they do it - there's no doubt about it," said Michael Boldin, tive director of the Tenth Amendment Center, a Los Angeles-based entity that promotes states'
exe
righ s . "There ' s no federal court in the country that's going to say that a state can pull this off. "
Yet tates may never need t o prosecute federal agents i n order t o make their point. If eno ug h states resi , "it's going to be very difficult for the federal government to force their l aws down our throats,"
Bol in said.
bill nullifying federal gun laws. whi le , thousands of people have sent online messages to the governor 's office about the legi lation.
Mis ouri 's governor has not said whether he will sign or veto the
Me
Signi g the measure "will show other states how to resist the tyrany of federal bureaucrats who want to ro you of your right to self-defense," said one message, si gned by Tim and Arlena Sowash, who gun shop in rural Stover, Mo. 路
e? dang time! People need to realize that the federal government does not have our best interest in If we can't take our country back with elections, maybe we can take parts back with nullification