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ESUO: Metabolic endotoxemia and male infertility

Dr. Alekzander Khelaia ESUO, Board Member National Centre of Urology European University Tbilisi (GE)

alekoxelaia@ gmail.com

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Infertility is a global health problem which affects 10–15% of couples in the reproductive age. There is growing evidence to support the theory that lifestyle factors affect male fertility through alterations in endocrine profiles, spermatogenesis and sperm function. Thus, the identification of factors contributing to infertility may be critical to offer simpler and/or more effective therapeutic options than those currently available. The increasing worldwide prevalence of metabolic syndrome (MetS), especially in younger populations, is a risk factor for fertility disorders. However, it remains unclear whether there is a direct correlation between MetS and male infertility. Obesity and a diet high in fat or calories, which is typically consumed by obese individuals, have been reported to cause a breakdown in the normal gut mucosal barrier function, leading to the passage of gut bacteria membrane remnants into the systemic circulation, initiating a chronic state of systemic inflammation. Inflammation, particularly in adipose tissue, has been implicated in diet and obesity-related insulin resistance in experimental models. [1]

High-fat diet Gómez-Elías et al [2] induced a metabolic syndromelike condition in experimental models. (C57BL/6xBALB/c) F1 male mice were fed a high-fat diet (HFD, 30% fat) for 19 weeks, while controls received a normal-fat diet (NFD, 6% fat). HFD-fed animals exhibited increased body weight, hypercholesterolemia, hyperglycaemia and glucose intolerance. HFD-fed males exhibited a higher amount of gonadal fat, proposed to increase testicular and epididymal temperature, thus affecting sperm production, maturation and storage. However, HFD-fed mice exhibited a decrease in epididymal weight, consistent with the lower epididymal sperm count. Also, sperm analysis showed significant differences between HFD and NFD-fed mice in cauda epididymal sperm count, sperm viability, morphology and progressive motility.

Impairment of sperm motility Ning Ding et al. [3] investigate if HFD-induced gut microbiota dysbiosis can functionally influence spermatogenesis and sperm motility. Faecal microbes derived from the HFD-fed or NFD-fed male mice were transplanted to the mice maintained on NFD. The gut microbes, sperm count and motility were analysed. Transplantation of the HFD gut microbes into the NFD-maintained (HFD-FMT) mice resulted in a significant decrease in spermatogenesis and sperm motility, whereas similar transplantation with the microbes from the NFD-fed mice failed to do so. Transplantation with HFD microbes also led to intestinal infiltration of T-cells and macrophages as well as a significant increase of pro-inflammatory cytokines in the epididymis, suggesting that epididymal inflammation has likely contributed to the impairment of sperm motility. RNA-sequencing revealed significant reduction in the expression of those genes involved in gamete meiosis and testicular mitochondrial functions in the HFD-FMT mice. Ning Ding et al. revealed an intimate linkage between HFD-induced microbiota dysbiosis and defects in spermatogenesis with elevated endotoxin, dysregulation of testicular gene expression and localised epididymal inflammation as potential causes. [3]

Metabolic endotoxemia Obesity and a high fat/high calorie diet are both reported to result in changes in gut bacteria and intestinal wall permeability, leading to the passage of bacterial endotoxin (lipopolysaccharide-LPS) from the gut lumen into the circulation (metabolic endotoxemia), where it initiates systemic inflammation. Endotoxin is known to reduce testosterone production by the testis, both by direct inhibition of Leydig cell steroidogenic pathways and indirectly by reducing pituitary LH drive, thereby also leading to a decline in sperm production. Gramnegative bacteria, which comprise 70% of the total bacterial load in the human gut, contain a potent immune stimulant in their cell wall, referred to as lipopolysaccharide (LPS) or endotoxin.

High fat or high calories Animal experiments and human observational studies have shown that consumption of diets containing either high fat or a high number of calories leads to significant changes in gut bacterial populations. It also increases the circulating levels of plasma endotoxin, implying a breakdown in gut mucosal wall integrity and the passage of Gram-negative bacteria membrane potent immune stimulant into the systemic circulation. Interestingly, the magnitude of this ‘metabolic endotoxemia’ is reported to be more pronounced in mice placed on a high fat diet than on an isocaloric high carbohydrate diet. This suggests that dietary fat is more efficient in transporting bacterial endotoxin from the gut lumen into the circulation, mediated by transfer of endotoxin across the intestinal wall in lipid laden chylomicrons.

Intestinal permeability Furthermore, there are reports that a high fat diet unfavourably alters the gut microbial composition, leading to an increase in intestinal permeability due to disordered tight junction proteins (zonulin, occludin) and a reduction in the colonic mucous barrier. In his study, Kelton Tremellen postulated for the first time that in the gut transmucosal passage of bacterial lipopolysaccharide (LPS) from the lumen into the circulation is a key inflammatory trigger underlying male hypogonadism. [4] High-fat diet has a prominent role in increasing oxidative stress and lowering antioxidant effect.

“The increasing worldwide prevalence of metabolic syndrome (MetS), especially in younger populations, is a risk factor for fertility disorders.”

Diet composition and obesity There is mounting evidence that obesity has negative repercussions for reproductive physiology in males. A large part of this evidence has accumulated from animal studies employing diets high in fat and sugar (‘high fat’ or ‘western’ diets). While excessive fats and carbohydrates are supposed to be major determinants of diet-induced obesity, a growing body of research suggests that the relationships between diet composition and obesity are more complex than originally thought, involving interactions between dietary macronutrients. Elevation of reactive oxygen species (ROS) may have a detrimental effect on sperm quality and hence fertilisation potential. This is undoubtedly an important finding, given the clear negative impact of obesity on male reproduction and the strong relationship between diet and obesity risk.

Healthy reproduction diet However, there is no clear definition of what a ‘healthy diet’ for reproduction is. For most men, switching to a ‘healthy diet’ means reducing the intake of food containing saturated fat and added salt and sugars and eating a wider variety of unprocessed food (as recommended by nutritional dietary guidelines). There is a clear need to further explore how diet impacts male reproductive function in order to develop evidence-based preconception nutritional guidance for men. In their cohort study, Linn B. Hakonsen et al. observed that the altered androgen profile tends to improve following weight loss and that weight loss may potentially lead to improvement in semen quality. [5] In a pilot study of 37 infertile men, Karma L. Pearce et al. confirmed a significant positive correlation between body mass index (BMI), increased intestinal permeability (serum zonulin), metabolic endotoxemia (LBP), sperm DNA oxidative damage (seminal 8 OHdG) and increasing levels of sperm DNA fragmentation. Metabolic endotoxemia was positively correlated with increasing levels of sperm DNA oxidative damage. This relationship remained significant, even after adjustment for relevant confounders such as age, BMI and days of abstinence. These observations suggest that metabolic endotoxemia and its associated oxidative stress may be a key driver of sperm DNA damage in obese men. [6] Probiotics A recent study by Dardmeh et al. [7] demonstrated that probiotics could be an alternative solution in eliminating obesity drawbacks on semen quality. The study was performed on male mice to investigate the effect of probiotics (Lactobacillus rhamnosus) on sperm kinematic parameters, testicular weight, lipid profiles and reproductive hormones such as follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone. Probiotics have a positive effect on male fertility by either a direct or indirect influence. The direct effect improves spermatogenesis and maturation process whereas the indirect effect works out by eliminating the adverse effects of obesity and elevating the total antioxidant capacity. In another study, Amandine Everard et al. [8] demonstrated that prebiotic (oligofructose) treatment restored Akkermansia muciniphila abundance and improved gut barrier/gut permeability and metabolic parameters. A. muciniphila improved metabolic disorders in diet-induced obese mice, normalised diet-induced metabolic endotoxemia, adiposity, and the adipose tissue marker CD11c. Similarly, A. muciniphila treatment reduced body weight and improved body composition (fat mass/lean mass ratio) without changes in food intake. This study clearly demonstrated the lack of a direct relationship between the abundance of Gram-negative bacteria within the gut and metabolic endotoxemia (i.e. caused by serum LPS) because gut colonisation by A. muciniphila decreased metabolic endotoxemia arising on an HF diet. An explanation for this counterintuitive result may be that A. muciniphila regulates gut barrier function at various levels. So, according to previous data, gut microbiota contribute to gut barrier alterations during obesity and are the reason of metabolic endotoxemia.

Anti-oxidant probiotic strains A recent study was carried out by Valcarce et al. [9] to evaluate the effect of two selected anti-oxidant probiotic strains (Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347) on sperm criteria of asthenozoospermic men. Four parameters were evaluated: sperm motility, sperm viability, DNA fragmentation, and level of ROS. Viability was not affected while the other three tested parameters demonstrated a significant improvement after probiotic treatment. These findings support the importance of using probiotics to improve fertility of human males.

Placebo-controlled study Finally the first placebo-controlled study was conducted by Maretti and Cavallini10 and reported a significant augmentation in testosterone level and sperm quality of infertile men after using a combination of prebiotic/probiotic therapy. Data showed a direct impact of prebiotic/probiotic therapy on the function of the pituitary gland in terms of enhancing FSH and LH serum levels.

Since many studies show that prebiotics and probiotics are the key regulators of microbiota improvement, they may have an influential therapeutic impact on the above-mentioned disturbances (metabolic endotoxemia) and in this way may open a new avenue in the treatment of idiopathic male infertility.

References

1. Nehal N Mehta et al. Experimental endotoxemia induces adipose inflammation and insulin resistance in humans.

Diabetes 2010 Jan 59 (1): 172 -81. 2. Gomez-Elias et al. Association between high-fat diet feeding and male fertility in high reproductive performance mice. Scientific Reports | (2019) 9:18546 | https://doi.org/10.1038/s41598-019-54799-3 3. Ning Ding et al. Impairment of spermatogenesis and sperm motility by the high-fat diet – induced dysbiosis of gut microbes. Gut 2020;69:1608–1619. doi:10.1136/ gutjnl-2019-319127 4. Tremellen et al. Gut endotoxin leading to a decline in

Gonadal function (GELDING) – a novel theory for the development of late onset hypogonadism in obese men.

Basic Clin Androl 2016 26:7 5. Linn B. Hakonsen et al. Does weight loss improve semen quality and reproductive hormones? results from a cohort of severely obese men. Reproductive health 2011; 8:24 6. Karma L. Pearce et al. Obesity related metabolic endotoxemia is associated with oxidative stress and impaired sperm DNA integrity. Basic Clin Andrology 2019

May 13;29:6. 7. Dardmeh F. Lactobacillus rhamnosus PB01 (DSM 14870) supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model. 2017,

PLoS One 12(10):1–17 8. Amandine Everard. Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Proc Natl Acad Sci 2013 May 28; 110 (22). 9. Valcarce. Probiotic administration improves sperm quality in asthenozoospermic human donors. Benificial

Microbes 2017 8:2 10. Maretti C, Cavallini G The association of probiotic with a prebiotic (Flortec, Bracco) to improve the quality/quantity of spermatozoa in infertile patients with idiopathic oligoasthenoteratospermia: a pilot study. Andrology 2017 5:439–444

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