The following data is the detail translation from video taken in Dr.沈湯龍’s class for key points of 2nd molecular biology.
It is strongly suggested taken with textbook – Gene x
Ch-19
RNA-polymerase
Page
contents be aware of
??
transcription bubble
p.510
polymerase subunits 組合
p.511
σ factor 功能 (不是 ơρ 歐)
p.512
polymerase 是用 direct displacement 而非 sliding 移動
p.518
σ factor 的結構和其 domain 的功能
p.519
σ factor 的調控機制 (N-Terminals blocks….)
p.521&522
一些基礎分生技術 (e.g footprinting?? Modification mapping binding site in two ways?)
p.524
RNA polymerase 結構 (DNA 進來碰到結構順序: bridge-wall-rudder , what r they??)
p.526
what should RNA terminator look like?
p.527&528
what should rho protein look like and how it acts ??
p529
what is “antitermination complex” ?
Ch-26 The Operon Page
contents be aware of
p.738
basic concept of regulation
p.739
fig26-4 is important (draw)
p.740
fig26-5 is important (draw),which is about the full structure of lac operon (include the protein it codes?)
p.743
section 26-4, the allosteric control of repressor protein
p.744~745
genetic approach to study lac operon, remember the mutants!!
p.750
about 3 operator (o1,o2,o3) it binds and the repression efficiency縲［echanic to regulate
p.751
three important factors for repressor efficiency 1.genome size, 2.repressor number, 3.affinity (by inducers) How to make the calculation ??
p.753
induced or repressed ? where should u put the CAT (CRP) into the lac operon ??
p.757~759
how E.coli control trp operon (draw) what is leader peptide sequence and how it works
p.760
how is translation by proteins ? what is autoregulation??
Ch-27 Phage strategies Page
contents be aware of
p.772
initial control (change σ factor?) and
termination control
(anti-terminator protein stop transcription termination) p.776~778
section 27-7
lytic cycle
section 27-8
lysogenic cycle
p779
section 27-9
what is immunity region??
p.781
competetion between “cro” and “cI”
P.782
Binding affinity of o1,o2,03.
p.784
competition in lytic cycle and lysogenetic cycle
p.785~787
section 27-15 CII, CIII –establishment of lysogeny CI
-maintenance
Section 27-16 What is CII function ?? (Promoter RE is poor fit (atypical -10 &-35 sequences) -require CII (positive regulatr) to help RNA pol to bind.) p.789
cro repressor for lytic cycle (fig 27.34 (lytic) and cf fig 27.33( lysogenetic ) important , fig 27.35(sum up))
Ch-30 RNA Regulators Page
contents be aware of
p.863
what is ribozyme? What is aptamer ?
p.867-868
noncoding RNA , and make example from bacteria Srna (oxyS RNA)
P.870-871
fig 30.9 and 30.10 is important How miRNA, siRNA, piRNA be synthized and where are they from, what is the enzyme and protein in the process??
P.873
translation repression concepts