Insight
2
Issue May-June 2019
Parkinson's disease research breakthroughs: Partnering with the Michael J. Fox Foundation Addictive Brain project: platform for clarifying misconceptions about science
Get to the root of Alzheimer's disease with our pathway poster at www.abcam.com/Alzheimer
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Then there’s the new tech use to generate custom antibodies
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Antibodies for Parkinson's disease targets
To provide the tools you need for Parkinson’s disease research, we partnered with The Michael J. Fox Foundation (MJFF) to develop rabbit monoclonal antibodies for leucine-rich repeat kinase 2 (LRRK2), alpha-synuclein, DJ-1, RAB8A, and RAB10. We've put together a video to showcase what the partnership means for the advancement of future Parkinson's disease research breakthroughs. Watch the video here. Featured Parkinson's disease antibodies Antibody
Antibody Name
Species
Clone ID
Applications
Ab no.
LRRK2
LRRK2
Hu, Ms, Rt
MJFF2 (c41-2)
ICC/IF, IHC-FrFl, IHC-P, IP, WB
ab133474
LRRK2
Hu, Ms, Rt
MJFF3 (c69-6)
ICC/IF, IHC-P, IP, WB
ab133475
LRRK2
Hu, Ms, Rt
MJFF4 (c81-8)
ICC/IF, IHC-P, IP, WB
ab133476
LRRK2
Hu, Ms
MJFF5 (68-7)
WB
ab181386
LRRK2
Hu, Ms
UDD3 30(12)
ICC/IF, IHC-P, WB
ab133518
LRRK2 (phospho S910)
Hu
UDD1 15(3)
WB
ab133449
LRRK2 (phospho S935)
Hu, Ms
UDD2 10(12)
ICC/IF, WB
ab133450
LRRK2 (phospho S955)
Hu, Ms
MJF-R11 (75-1)
WB
ab169521
LRRK2 (phospho S973)
Hu
MJF-R12 (37-1)
WB
ab181364
LRRK2 (phospho T1410)
Hu, Ms, Rt
MJFR4-25-5
WB
ab140107
LRRK2 (phospho T1491)
Hu
MJFR5-88-3
WB
ab140106
LRRK2 (phospho T1503)
Hu
MJF-R6 (227-1a)
WB
ab154423
LRRK2 (phospho S1292)
Hu
MJFR-19-7-8
WB
ab203181
LRRK2 (phospho T2483)
Hu
MJF-R8
WB
ab156577
Alpha-synuclein
Hu, Drosophila melanogaster
MJFR1
ELISA, Flow Cyt, ICC/IF, IHC-P, IP, WB
ab138501
Alpha-synuclein (phospho S129)
Hu
MJF-R13 (8-8)
WB
ab168381
Alpha synuclein filament conformation specific
Hu, Ms, Rt
MJFR-14-6-4-2
ICC/IF, IHC-P, dot blot
ab209538
DJ-1
DJ1 (PARK7)
Hu
MJF-R16 (66-5)
WB
ab169520
Rab8A and Rab10
Anti-RAB8A (phospho T72)
Mo, Hu
[MJF-20-25-2]
WB, Dot blot
ab230260
Anti-RAB10 (phospho T73)
Mo, Hu
[MJF-21-108-10]
WB, Dot blot
ab230261
Anti-RAB8A
Mo, Rt, Hu
[MJF-22-74-3]
WB, IP
ab237702
Anti-RAB10
Mo, Rt, Hu
[MJF-23-32-5]
IP, Flo Cyt, ICC/IF, WB
ab237703
Anti-RAB10 (phospho T73)
Mo, Hu
[MJF-R21-22-5]
WB, Dot Blot, ICC/IF
ab241060
Anti-RAB29 (phospho T71)
Hu
[MJF-R24-17-1]
Dot Blot, WB
ab241062
Alpha-synuclein
More information at
www.abcam.com/Parkinson-disease
Conjugated primary antibodies against neuronal and glial markers Advantages of directly conjugated neural markers - Speed up your staining protocol. Directly conjugated antibodies already have the Alexa Fluor® dye, fluorescent protein or enzyme attached, so you can skip that secondary antibody staining step. - Easily design multicolour experiments. Avoid the stress of finding primary antibodies from different host species. Directly conjugated antibodies allow you to side-step secondary antibody cross-reactivity.
Immunohistochemistry of formalin-fixed paraffin-embedded rat cerebellum tissue sections stained with anti-NeuN [EPR12763] – neuronal marker (Alexa Fluor® 647) (ab190565). DAPI-only control is from another section and controls for tissue autofluorescence.
Find out more
Featured conjugated primary antibodies Cell type
Target
Available conjugate(s)
Reference link
Immature neurons
Beta III tubulin
Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 594, Alexa Fluor® 647, FITC, HRP
www.abcam.com/b-tubulin
Stathmin 1
Alexa Fluor® 488, Alexa Fluor® 647, HRP, PE
www.abcam.com/stathmin-1
NeuN
Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 594 Alexa Fluor® 647, biotin
www.abcam.com/NeuNs
MAP2
FITC, biotin
www.abcam.com/MAP2
Synaptophysin
Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 647, FITC, HRP
www.abcam.com/synaptophysin
160 KDa Neurofilament medium/ 200 KDa Neurofilament heavy
Alexa Fluor® 594, Alexa Fluor® 647, HRP
www.abcam.com/neurofilaments
PSD95
Alexa Fluor® 488, Alexa Fluor® 647, FITC, HRP, biotin
www.abcam.com/PSD95
GFAP
Alexa Fluor® 405, Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 594, Alexa Fluor® 647, Cy3, HRP
www.abcam.com/GFAP
S100 beta
Alexa Fluor® 488, Alexa Fluor® 568, Alexa Fluor® 647, FITC, HRP, biotin
www.abcam.com/S100
Glutamine synthetase
HRP, PE, biotin
www.abcam.com/GS
MBP
HRP, biotin
www.abcam.com/MBP
GAP43
Alexa Fluor® 488, Alexa Fluor® 647, HRP, PE
www.abcam.com/GAP43
p75 NGF receptor
Alexa Fluor® 488, Alexa Fluor® 647, FITC, PE, biotin
www.abcam.com/P75-NGF
CD68
Alexa Fluor® 488, Alexa Fluor® 647, FITC, PE, Biotin
www.abcam.com/CD68
Iba1
Alexa Fluor® 488, Alexa Fluor® 647, FITC
www.abcam.com/Iba1
CD11b
FITC, PE, biotin, APC
www.abcam.com/CD11b
Myelin oligodendrocyte glycoprotein (MOG)
Alexa Fluor 647®, HRP
www.abcam.com/MOG
Mature Neurons
Astrocytes Astrocytes
Schwann cells
Microglia
Oligodendrocytes
More information at www.abcam.com/Neural-markers
THE ADDICTIVE BRAIN The Addictive Brain is a project that focuses on science communication. Founded by Dr. Chinmaya Sadangi, a neuroscientist and a passionate science communicator, he aims to bridge the gap between scientists and the public by explaining science in ways that non-scientists can understand, “We aim to spread awareness about how scientists utilize tax payer’s money/ government funding to help develop a better world and encourage women and underrepresented minorities can get interested in studying STEM subjects.”
Why was The Addictive Brain set up? “One of the major factors that led us to create The Addictive Brain was the rising misconceptions about science. The non-scientists we speak with often have myths or misconceptions about science-related topics. “Often, people may read media reports and consider it to be true, whereas it’s far from the truth. For example, many people believe the myth that vaccination causes autism, and it was spread by some media article. And there are not just a few, but many such articles posted by nonreliable sources that spread myths. “We wanted to create a platform where scientists directly share their day to day experiences, studies, and educate people directly. For transparency, we also encourage direct interactions between our contributors and audience. We have been receiving great feedback so far and people wish to see so much more.”
About The Addictive Brain project
The Addictive Brain
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Abcam Tipbox
Faster ELISA with better results One-step sandwich ELISA delivers specific and sensitive results in 90 minutes Superior human PD-L1 quantitative assessment tool - Human PD-L1 SimpleStep ELISA Kit [28-8](ab214565) has an increased sensitivity compared to leading competitor.
Get sensitive quantification with one-step SimpleStep ELISAÂŽ kits - Human CXCL16 SimpleStep ELISA kit (ab187397) has an increased sensitivity compared to a conventional 3-step ELISA kit using a heterogeneous protocol.
Specificity for target proteins in complex biological samples - Only antibody combinations that give the highest overall signal are used for further development.
Featured neuroscience Simplestep ELISA kits Ab no.
Ab no.
www.abcam.com/simplestep
Neuroinflammation and Alzheimer’s disease
Aβ clearance
Release of proinflammatory mediator
Active microglia
IL-1β, IL-6, TNFα, IFNγ, MIP-1α, MIP-1β, RANTES
TREM2
Migrating microglia
Aβ plaque
Reactive astrocytes
Neuronal damage and death
GFAP
Ta MCP-1 gradient
Amyloidogenic APP processing
Aβ
Caspase 3
GSK3β
Caspase 7
β-secretase γ-secretase
Tau fila
P Cdk5
Cdk5
p35
p25 Calpain
Blood brain barrier
AICD C99 APP
P
Proteosomal degradation
P
P
P
P P
CCR2 gradient
P
P
Ubiquitin
Oligomeri Tau
P
P
P
P
P
P
P
P P
P
P P
P
P
P
Autophagy
P
P
P
P
P P
Infiltration of monocytes and macrophages
Tau on microtubules
Tau hyperphosphorylation
Tau
Axon
To find out more, please visit www.abcam.com/neuroinflammation Copyright © 2017 Abcam, All rights reserved. Poster compiled with help from Dr John-loannis Sotiropoulos, ICVS.
Get to the root cause of neurodegeneration with these tools Amyloid beta
rs
S, MCP1, M-CSF
Aβ peptide (1-42)
Quickly induce neurotoxicity and neurodegeneration with this high purity Aβ fragment.
ab120301
CRANAD-58
Image Aβ in real time with this non-toxic, cell-permeable probe suitable for in vitro and in vivo imaging.
ab146926
To find out more about amyloid beta probes, visit www.abcam.com/amyloid-probes
Tau
Synapse loss
Anti-Tau (phospho S396) antibody
Quickly detect phospho S396 tau with this versatile antibody. Available in 10 μl trial format.
ab109390
LMTX
Target tau tangles and facilitate aggregate clearance with this tau aggregation inhibitor.
ab223882
To learn about other products to study tau, visit www.abcam.com/tau
Clq
C3
Glia Distinguish microglia from macrophages and other immune cells with this stable and specific microglial marker.
Anti-TMEM119 antibody
au neurofibrillary tangles (NFTs)
CR3
Anti-GFAP antibody
ab209064
ab7260
marker.
For more help choosing neural markers, visit www.abcam.com/glia
Proinflammatory mediators Mouse TNF alpha ELISA Kit
Detect proinflammatory mediators, such as TNF-alpha, in just 90 minutes with a SimpleStep ELISA® kit.
ab208348
To discover the range of SimpleStep ELISA kits, visit www.abcam.com/simplestep-elisa
Microglial complement activation
paired helical aments (PHFs)
Ca2+ Ca
ic
+
Ca P
+
Axon terminal
+
Glutamate
Soluble Aβ oligomer
NMDA receptor
P
P
ab213392 ab213397
To learn more about Fireplex immunoassays, visit www.abcam.com/fireplex-immunoassays
P
P
P
Ca
Profile multiple cytokines at once with a multiplex panel or design your own.
P
P P
+
Ca + Ca
Mouse and human key cytokine panels
AMPA receptor
P
Ca
P
Tau mislocalization to dendritic spine
Ca P
Ca
+
ROS
Ca
P
P
+
+
Ca Ca +
P
FYN
Membrane damage
+
Ca
PSD95
FYN
+
+
FYN
P
P
P
P
P P
Ca2+ overload
Dendritic spine
Apoptosis
Isotype Control Antibodies Alexa FluorÂŽ dyes, HRP, Biotin, Phycoerythrin, FITC, Agarose, and other formats. Explore our 245 isotype controls with our isotype control selector More information, please visit
www.abcam.com/isotype-control
Featured isotype controls
Just antibodies + PBS Carrier free formulations No azide, no BSA, no glycerol Just antibodies + PBS
The flexibility you need
Build your own ELISA-based assay
Recombinant antibodies
PBS-only antibody pairs
Free from BSA, sodium azide, and glycerol
Recombinant capture and detector antibodies
Supplied at 1 mg/mL concentration
Free form BSA, sodium azide and glycerol
Conjugation ready
Suitable for sandwich immunoassays
Ideal for CyTOFÂŽ and multiplex IHC
Available as 10 x 96 wells plate
Low-endotoxin (<1 EU/mL) options available
Find out how our carrier-free formulations can offer more flexibility for your experiments at
www.abcam.com/carrier-free
Small molecule compounds High purity
Minimal off-target effects
Enzyme inhibitors and activators
– – – – –
Clathrin CaMK AMPK HDAC Rho
– – – –
MAPK Caspase PI3K/Akt ATPase
Consistent experiments
Receptor agonists and antagonists
– – – – –
Glutamate GPCR Muscarinic Adenosine Adrenoceptors
– – – –
GABA Nicotinic Purinergic Serotonin
Ion channel blockers and modulators
– – – –
Sodium Calcium Ionophores Chelators
– Potassium – TRP – Indicators
Highlights Small molecule compound
Target function
Ab no.
AICAR
AMP analog for cell culture
ab120358
Bafilomycin A1
Selective (V)-ATPase inhibitor
ab120497
BrdU
Cell proliferation reagent
ab142567
FK506 (Tacrolimus)
Potent immunosuppressant
ab120223
Forskolin
Adenylyl cyclase activator
ab120058
Fluo-8 AM
Green fluorescent calcium binding dye
ab142773
Ionomycin (calcium salt)
Ca ionophore
ab120116
PMA (phorbol myristate acetate)
Protein kinase C (PKC) activator
ab120297
2+
Inhibitor cocktails Protect your samples from degradation with pre-made inhibitor cocktails.
www.abcam.com/inhibitor-cocktails Abcam exclusive Clathrin inhibitor: Pitstop® 2 (ab120687) Dynamin inhibitor: Dyngo® 4a (ab120689)
Copyright © 2019 Abcam. All rights reserved.
