Product Catalogue 1st edition March 2011
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Contents Page Introduction to BBI
3
How to Order
3
Gold Colloid
4
Gold Conjugates
5
Silver Colloid
6
Gold Nanorods - Nexus RodsTM
7
Gold Nanobeads - Nexus BeadsTM
8
Microarrays
10
Miscellaneous Products
11
BBI Online
12
a Technical forum
a Order online
2
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Introduction Founded in 1986, BBI is the world leader in the manufacture of high quality gold and silver reagents for research and diagnostic applications. Our policy has always been to provide outstanding products, manufactured and tested with the highest emphasis placed on reliability, sensitivity and reproducibility. In addition, our vast experience enables us to provide our customers with a comprehensive technical support programme. Detailed within this catalogue, our Life Science product range is made up of gold and silver colloid and gold conjugates, as well as our Nexus™ range comprising gold nanorods and gold nanobeads. We also provide a complete creative conjugation service, developing gold conjugates outside of our catalogued range. We welcome discussion with our customers to consider the possibilities of creating any type of gold conjugate to meet your requirements.
How to order
Technical Support
Orders can be made by sending a Purchase Order to:
Take advantage of our technical support programme
+44 (0) 29 2074 7242
info@buybbi.com
Or online:
www.buybbi.com
info@bbigold.com
+44 (0) 29 2074 7232
Ongoing Requirements Contact us to set up a proforma account
+44 (0) 29 2074 7232
info@buybbi.com
Larger volumes are available on request All prices stated are in pounds sterling. All rights reserved BBInternational March 2011.
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3
Gold Colloid BBI’s unique recipe gold colloid has been market leading for over 25 years due to its superior quality, consistency and performance, offering excellent stability and sensitivity characteristics. There are several advantages of choosing BBI’s gold colloid: • Lowest available coefficient of variation (CV) • The choice of particle size makes it possible to study antigens microscopically • Allows multiple labelling of antigens on tissue sections • Labelling is completely unequivocal • Provides a permanent label
• Quantitation under the electron microscope is possible due to its particulate nature • The optical properties mean there is a variation in colour from red to pink to orange depending on particle size, a property that can be successfully exploited in a range of applications • Known to be catalytically active for a range of commercially important reactions
Pricing
20ml
Gold Colloid Array Gold Colloid
£37.00 See page 10
100ml
500ml
£146.00
£487.00
Sizes Code
Size
Code
Size
EMGC2
2nm
EMGC50
50nm
EMGC5
5nm
EMGC60
60nm
EMGC10
10nm
EMGC80
80nm
EMGC15
15nm
EMGC100 100nm
EMGC20
20nm
EMGC150
EMGC30
30nm
EMGC200 200nm
EMGC40
40nm
EMGC250
150nm 250nm
Gold Colloid Starter Packs A wide range of particle sizes can be conjugated to proteins and macromolecules, and first time gold users or those trying out a new process may be unsure of which size to purchase. Our gold colloid starter packs provide a range of particle sizes allowing a comprehensive evaluation.
Gold Colloid 5nm/10nm/20nm/40nm Gold Colloid 20nm/40nm/60nm/80nm
4
Code
Volume
Price
GCIKITLIFE GCIKITDIAG
20 ml of each 20 ml of each
£131.00 £131.00
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Gold Conjugates A colloidal gold conjugate consists of a suspension of gold particles coated with a selected protein or macromolecule (such as an antibody). BBI has a range of EM grade products that combine different antibodies on the range of particles predominantly 2, 5, 10, 15, 20, 30 & 40nm. BBI’s gold conjugates provide the following benefits.
Pricing
• Binding to BBI’s superior quality gold colloid ensures a very low level of clustering while maintaining high level stability and sensitivity • Only highest quality antibodies and proteins are used, meaning consistently reliable products • BBI’s gold conjugates average 95% singlet particles
0.25ml
EM and all Conjugates (except EM. BSA) EM. BSA Conjugates Microarray Conjugates
1ml
£75.00 £53.00 £121.00
£224.00 £149.00 £424.00
Conjugate Key BSA Bovine Serum Albumin
GAT Goat anti Rat IgG
CGC Cationic Colloidal Gold
GFAR Goat F(ab)’2 anti Rabbit IgG
GAF Goat anti Mouse IgG & IgM
GMHL Goat anti Mouse IgG (H+L)
GAG Goat anti Guinea Pig IgG
PAG Protein A
GAM Goat anti Mouse IgG Fc (Gamma) Specific
RAG Rabbit anti Goat IgG
GAR Goat anti Rabbit IgG
STP Streptavidin
Code
Size
Code er EM. GAR5 Best Sell er EM. GAR10 Best Sell er EM. GAR15 Best Sell
Size
Code
Size
5nm
EM. PAG5
5nm
10nm
EM. PAG10
10nm
EM. BSA5
5nm
EM. BSA10
10nm
EM. CGC5
5nm
15nm
EM. PAG15
15nm
EM. CGC10
10nm
EM. GAR20
20nm
EM. PAG20
20nm
EM. CGC15
15nm
EM. GAR30
30nm
EM. RAG5
5nm
EM. CGC20
20nm
EM. GAR40
40nm
EM. RAG10
10nm
EM. GAF10
10nm
EM. GAT10
10nm
EM. RAG15
15nm
EM. GAG5
5nm
EM. GFAR5
5nm
EM. RAG20
20nm
EM. GAG10
10nm
EM. GFAR10
10nm
EM. STP2
2nm
EM. GAM2
2nm
5nm
EM. STP5
5nm
EM. GAM5
5nm
10nm
EM. STP10
10nm
EM. GAM10
10nm
15nm
EM.STP15
15nm
EM. GAR2
2nm
20nm
EM.STP20
20nm
er EM. GMHL5 Best Sell er EM. GMHL10 Best Sell er EM. GMHL15 Best Sell EM. GMHL20
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5
Silver Colloid The use of silver can potentially lead to improved sensitivity where silver nanoparticles demonstrate a stronger, more distinctive SPR spectrum than the corresponding gold particles. Use of silver in place of gold can potentially result in a more defined wavelength shift, and broadens the range of optical properties available – an advantage for applications where multiplexing is needed.
Pricing
20ml
Silver Colloid Array Silver Colloid
£37.00 See page 10
100ml
500ml
£146.00
£487.00
Sizes Code
Size
Code
Size
EMSC20
20nm
EMSC60
60nm
EMSC40
40nm
EMSC80
80nm
CREATIVE CONJUGATION SERVICE BBI are experts in creative conjugation.
Antibody Purification
Determine Antibody Titre
Let us conjugate your antibody to gold colloid. • 3ml finished conjugate • Technical advice and ongoing support
Talk to us about your requirements Tel: +44 (0) 29 2074 7232 info@buybbi.com
6
Manufacture and Concentrate Conjugate
Column Chromatography
Binding Analysis
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Nexus RodsTM Pricing Nexus Rods
TM
50 OD-ml
250 OD-ml
£300.00
£1500.00
Our range of Nexus Rods™ combines the revolutionary optical, photothermal, and mechanical properties inherent in the gold nanorod structure with a well characterized highly customisable surface. This makes them suitable for directing the bioactivity of gold nanorods or for conjugating them to a wide range of molecules. Resulting in better extinction, absorption, and scattering at tunable wavelengths, BBI’s Nexus Rods™ bring many benefits to optical, photothermal, and material methodology.
Amine-Polymer Size
(Volume: 1ml)
Codes
Neutravidin-Polymer Size
(Volume: 1ml)
Codes
550nm
60-R-PA-550-50
60-R-PA-550-250
550nm
60-R-PN-550-50
60-R-PN-550-250
600nm
60-R-PA-600-50
60-R-PA-600-250
600nm
60-R-PN-600-50
60-R-PN-600-250
650nm
60-R-PA-650-50
60-R-PA-650-250
650nm
60-R-PN-650-50
60-R-PN-650-250
700nm
60-R-PA-700-50
60-R-PA-700-250
700nm
60-R-PN-700-50
60-R-PN-700-250
750nm
60-R-PA-750-50
60-R-PA-750-250
750nm
60-R-PN-750-50
60-R-PN-750-250
(Volume: 1ml)
Methyl-Polymer
Biotin-Polymer Size
Codes
Size
(Volume: 1ml)
Codes
550nm
60-R-PB-550-50
60-R-PB-550-250
550nm
60-R-PM-550-50
60-R-PM-550-250
600nm
60-R-PB-600-50
60-R-PB-600-250
600nm
60-R-PM-600-50
60-R-PM-600-250
650nm
60-R-PB-650-50
60-R-PB-650-250
650nm
60-R-PM-650-50
60-R-PM-650-250
700nm
60-R-PB-700-50
60-R-PB-700-250
700nm
60-R-PM-700-50
60-R-PM-700-250
750nm
60-R-PB-750-50
60-R-PB-750-250
750nm
60-R-PM-750-50
60-R-PM-750-250
Carboxyl-Polymer Size
(Volume: 1ml)
Codes
550nm
60-R-PC-550-50
60-R-PC-550-250
600nm
60-R-PC-600-50
60-R-PC-600-250
650nm
60-R-PC-650-50
60-R-PC-650-250
700nm
60-R-PC-700-50
60-R-PC-700-250
750nm
60-R-PC-750-50
60-R-PC-750-250
BBI FACT: Custom Sizes In addition to our catalogue products, BBI are able to manufacture gold particles to custom diameters, up to 600 nm.
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7
Nexus BeadsTM Pricing Nexus Beads
TM
50 OD-ml
250 OD-ml
£300.00
£1500.00
(Volume: 1ml)
Biotin-Polymer
BBI’s range of Nexus Beads™ are manufactured using a proprietary method that results in exact and highly monodisperse sizes and shapes, indcluding a covalent bonded polymer cage with carboxyl, methyl, biotin, or neutravidin terminal groups. This formulation has been optimized for in vitro use.
Amine-Polymer Size
8
Codes
Size
(Volume: 1ml)
Codes
5nm
60-S-PA-5-50
60-S-PA-5-250
5nm
60-S-PB-5-50
60-S-PB-5-250
10nm
60-S-PA-10-50
60-S-PA-10-250
10nm
60-S-PB-10-50
60-S-PB-10-250
20nm
60-S-PA-20-50
60-S-PA-20-250
20nm
60-S-PB-20-50
60-S-PB-20-250
30nm
60-S-PA-30-50
60-S-PA-30-250
30nm
60-S-PB-30-50
60-S-PB-30-250
40nm
60-S-PA-40-50
60-S-PA-40-250
40nm
60-S-PB-40-50
60-S-PB-40-250
50nm
60-S-PA-50-50
60-S-PA-50-250
50nm
60-S-PB-50-50
60-S-PB-50-250
60nm
60-S-PA-60-50
60-S-PA-60-250
60nm
60-S-PB-60-50
60-S-PB-60-250
70nm
60-S-PA-70-50
60-S-PA-70-250
70nm
60-S-PB-70-50
60-S-PB-70-250
80nm
60-S-PA-80-50
60-S-PA-80-250
80nm
60-S-PB-80-50
60-S-PB-80-250
90nm
60-S-PA-90-50
60-S-PA-90-250
90nm
60-S-PB-90-50
60-S-PB-90-250
100nm
60-S-PA-100-50
60-S-PA-100-250
100nm
60-S-PB-100-50
60-S-PB-100-250
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Nexus BeadsTM Carboxyl-Polymer Size
(Volume: 1ml)
Codes
Methyl-Polymer Size
(Volume: 1ml)
Codes
5nm
60-S-PC-5-50
60-S-PC-5-250
5nm
60-S-PM-5-50
60-S-PM-5-250
10nm
60-S-PC-10-50
60-S-PC-10-250
10nm
60-S-PM-10-50
60-S-PM-10-250
20nm
60-S-PC-20-50
60-S-PC-20-250
20nm
60-S-PM-20-50
60-S-PM-20-250
30nm
60-S-PC-30-50
60-S-PC-30-250
30nm
60-S-PM-30-50
60-S-PM-30-250
40nm
60-S-PC-40-50
60-S-PC-40-250
40nm
60-S-PM-40-50
60-S-PM-40-250
50nm
60-S-PC-50-50
60-S-PC-50-250
50nm
60-S-PM-50-50
60-S-PM-50-250
60nm
60-S-PC-60-50
60-S-PC-60-250
60nm
60-S-PM-60-50
60-S-PM-60-250
70nm
60-S-PC-70-50
60-S-PC-70-250
70nm
60-S-PM-70-50
60-S-PM-70-250
80nm
60-S-PC-80-50
60-S-PC-80-250
80nm
60-S-PM-80-50
60-S-PM-80-250
90nm
60-S-PC-90-50
60-S-PC-90-250
90nm
60-S-PM-90-50
60-S-PM-90-250
100nm
60-S-PC-100-50
60-S-PC-100-250
100nm
60-S-PM-100-50
60-S-PM-100-250
Neutravidin-Polymer Size
(Volume: 1ml)
Codes
5nm
60-S-PN-5-50
60-S-PN-5-250
10nm
60-S-PN-10-50
60-S-PN-10-250
20nm
60-S-PN-20-50
60-S-PN-20-250
30nm
60-S-PN-30-50
60-S-PN-30-250
40nm
60-S-PN-40-50
60-S-PN-40-250
50nm
60-S-PN-50-50
60-S-PN-50-250
60nm
60-S-PN-60-50
60-S-PN-60-250
70nm
60-S-PN-70-50
60-S-PN-70-250
80nm
60-S-PN-80-50
60-S-PN-80-250
90nm
60-S-PN-90-50
60-S-PN-90-250
100nm
60-S-PN-100-50
60-S-PN-100-250
BBI FACT: Nexus The absorption and scattering sections of Nexus Beads can be up to two orders of magnitude greater than nanoparticles
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9
Microarray Gold & Silver Colloid In the Microarray market, gold with its unique properties has emerged as an alternative to fluorescence detection.
Key Features
BBInternational’s Microarray gold is the ideal solution for overcoming many of the problems associated with fluorescence detection methods, including low sensitivity, signal intensity, reduction due to photochemical bleaching, quenching and expensive image analysis systems.
• Highly Specific • Reproducible • Stable • Highly Sensitive • Reliable
Code
Size
Volume
ArrayGC2
2nm
20ml
ArrayGC5
5nm
20ml
ArrayGC10
10nm
20ml
ArrayGC15
15nm
20ml
ArrayGC20
20nm
20ml
ArrayGC30
30nm
20ml
ArrayGC40
40nm
20ml
ArrayGC50
50nm
20ml
ArrayGC60
60nm
20ml
ArrayGC80
80nm
20ml
ArrayGC100
100nm
20ml
ArrayGC150
150nm
20ml
ArrayGC200
200nm
20ml
ArrayGC250
250nm
20ml
Code
Size
• Low CV (<8%)
Volume
ArraySC20
20nm
20ml
ArraySC40
40nm
20ml
ArraySC60
60nm
20ml
ArraySC80
80nm
20ml
10
• Perfectly Spherical Shapes (≤95 %)
Pricing Microarray Gold Colloid Microarray Silver Colloid
20ml £110.00 £110.00
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Miscellaneous Products Pricing
20ml Code
100ml Price
Silver Kit EM/LM (15ml)
SEKL15
£78.00
Test Strips (Silver Kits)
SETS10
£20.00
Blotting Silver (250ml/kit)
SEKB250
£107.00
Unicryl (250ml)
BA250
£146.00
Protogold (500ml/kit)
PRO500
£107.00
Test Strips (Protogold)
SETPG10
£20.00
Biobond (20ml)
BB20
£36.00
Biomount (100ml)
BM100
£25.00
BSA 10g (powder)
BSA10
£39.00
Fish Gelatin (10ml)
GEL10
£20.00
Tween 20 (10ml)
T20
£9.00
Raman Spec Gold Colloid (150ul)
GC.150ul
£182.00
Raman Spec Silver Colloid (150ul)
SC.150ul
£182.00
Gold Colloid Starter Pack
save over
10%
on list price
Start your evaluation of varying colloid sizes with this versatile pack First time gold users or those trying out a new process may be unsure of which size to purchase. Our gold colloid starter pack provides a range of particle sizes to allow a comprehensive evaluation, comprising 20ml of each size.
5nm • 10nm • 20nm • 40nm
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11
BBI Online BBI’s Technical Forum
• Technical support
• R&D ideas
• New product ideas
• Interaction with fellow researchers
• Industry knowledge • Technical questions
Visit BBI’s forum and get involved in all the latest Life Science discussions!
• Your feedback
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