33rd Japan Ozone Association, Kyoto, Japan
IOA OZONE NEWS 2024
OZONE NEWS
Volume 52 / No. 4 • July 2024
Editor-in-Chief: Dr. Saad Jasim
Ozone News (ISSN 1065-5905) is a bimonthly publication of the International Ozone Association (IOA). Annual subscription rate: $150.00. For editorial and advertising information, please contact: International Ozone Association/Editorial Office
Attn: Dr. Saad Jasim, P.Eng.
4483 Cherry Hill Road
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Canada
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Email: saadjasim@ioa-pag.org and sjenvcons@gmail.com
Membership and Publication Information:
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As a member of the IOA, you’ll receive bimonthly issues of Ozone News, bimonthly issues of the technical journal Ozone: Science & Engineering (OS&E), and IOA’s Publication Catalog, which includes worldwide conference proceedings, monographs, and special reprints. In addition, members receive discounts on IOA worldwide publications and meetings.
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For membership and publication information, please contact the IOA office nearest you:
Pan American Group (PAG)
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Fax: 33 (0) 5 49 45 40 60
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Nippon Islands Group (NIG)
Mr.. Tetsuya Tamura
Japan Ozone Association
Intelligent Flats 301, 10-10 Nihonbashi Tomizawa-cho
Chuko- Tokyo 103-0006, Japan
Tel: (81) 3 6661 1622
Fax: (81) 3 6661 1623
Email: tamura@j-ozone.org • Web: www.j-ozone.org
WELCOME
4 Index of Advertisers
List of Sponsors
5 Editor’s Note: 33rd Japan Ozone Association Conference
Mark Your Calendars
IOA & INDUSTRY NEWS
6 23rd Anniversary for Launching the Ozone Application for Drinking Water Treatment in Windsor, ON, Canada
8 Research to Address Climate Change Challenges
9 New Presidents Nominated for IOA and EA3G
10 Japan Ozone Association 33rd Annual Conference on Ozone Science & Technology, Kyoto, Japan
12 International Association for Great Lakes Research 67 Annual Conference
14 Middle East and North Africa Ozone Generator Market
17 Ozone in Medicine. Ozone as Redox Bioregulator. III. Virus Diseases
20 Welcome New Members
2024 CONFERENCES
19 American Water Works Association Water Quality Technology Conference
20 Iberoamerican Conference on Advanced Oxidation Technologies
21 IOA–International Ozone Association EA3G2024 Europe, Africa, Asia, Australasia
Group International Conference
22 International Ozone Association–Pan American Group (IOA-PAG) 2024 Conference
INDEX OF ADVERTISERS
Page 2 Air Physics Co., Ltd.
Page 4 ANSEROS Klaus Nonnenmacher GmbH
Page 5 Statiflo
Page 6 Plasma Technics, Inc.
Page 7 Teledyne API
Page 8 Tersano
Page 9 Mazzei
Page 12 BMT
Page 13 BMT
Page 24 AirSep
Copyright ©2024 International Ozone Association. All rights reserved. No part of this publication may be reproduced, stored, transmitted, or disseminated in any form or by any means without prior written permission from the International Ozone Association. The publisher assumes no responsibility for any statements of fact or opinion expressed in the published papers.
33rd Japan Ozone Association Conference
It was a great pleasure to attend the 33 Japan Ozone Association Conference in Kyoto, Japan as Editor-inChief for the Ozone News of the IOA, accompanied by Dr. Keisuke Ikehata, Chair, Publications Committee of the IOA, and Jim Lauria, Mazzei Injector Company. That joint effort will strengthen our professional communications and improve our networks to deliver the information of worldwide success of ozone applications dealing with many new challenges. Thanks to the leadership of the Japan Ozone Association for organizing a successful conference.
Dr. Saad Y. Jasim, P.Eng. Ozone News Editor-in-Chief International Ozone Association
Mark Your Calendar
August 11–15, 2024 — IWA World Water Congress and Exposition, Metro Toronto Convention Center, Toronto. www.worldwatercongress.org
August 25–29, 2024 — IOA-PAG Annual Conference, Palms Resort & Casino, Las Vegas, NV. www.ioapag.org
August/September 2025 — IOA World Congress, Atlanta, GA. www.ioa-pag.org
November 17–21, 2024 — American Water Works Association Water Quality Technology Conference, Schaumburg, IL. www.awwa.org
November 27–29, 2024 — Europe, Africa, Asia, Australasia Group 2024 International Conference (EA3G2024), Porto, Portugal. www.ioa-ea3g.org
Statiflo supply a range of custom designed Ozone contacting systems which continually meet and exceed our guaranteed 95% mass transfer efficiency.
Key benefits include:
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The Statiflo Gas Dispersion System (GDS) is often used by our customers to replace other high-maintenance and low-efficiency technologies, reducing overall costs and reducing your environmental impact.
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Dr. Saad Jasim, P.Eng.
23rd Anniversary for Launching the Ozone Application for Drinking Water Treatment in Windsor, ON, Canada
By Dr. Saad Jasim, P.Eng.
June 15, 2001 was the official start of the Ozone Application in Windsor, Ontario, Canada. I proposed the application in 1999, and it received unanimous approval by the Windsor Utilities Commissioners. That made Windsor the leader in the application of Ozone in Ontario, the Ozone application is an ideal application to have a “ Multi Barrier System”.
In 2000, the Walkerton outbreak of E. coli that caused the death of 7 people and the illness of approximately 2300 residents of the town of Walkerton was an eye opener that we should not take safe drinking water for granted. The Walkerton Inquiry indicated clearly the importance of having a Multi Barrier System.
The Ozone application in Windsor provides multiple advantages: Disinfection, Taste & Odour Control, Reduction of Disinfection By Products (DBPs), degradation and removal of Chemicals of Emerging Concern (Pharmaceuticals, Personal Care Products and Endocrine Disrupting Compounds), reducing of chemicals used, and degradation and removal of Cyanotoxins (dangerous toxins from the Harmful Algal Blooms). The Ozone application was able to reduce more than $247,000 from operation cost compared to the previous conventional application.
Recently, I am proud that ENWIN Utilities Ltd. and Mitacs have confirmed funding to a research project to evaluate the Simultaneous Oxidation and Removal
for 3 Cyanotoxins. The findings of that research will provide important information to optimize the operation of Ozone systems to achieve efficient degradation of these 3 Cyanotoxins.
• Water Treatment
• Semiconductor
• Medical, Pharmaceutical
• Food & Beverage
Providing
Teledyne
Research to Address Climate Change Challenges
A proposed research project titled “Simultaneous Removal/Degradation of Three Cyanotoxins in Drinking Water Source (Detroit River), using Ozone and Ozone Advanced Oxidation Process (AOP)” has been approved for funding by ENWIN Utilities Ltd., Windsor, Ontario and Mitacs.
This project aims to establish an effective operational standard for water treatment plants using ozone and/ or an ozone-based Advanced Oxidation Process (AOP) to oxidize the three cyanotoxins produced from harmful algal blooms.
Cyanotoxins are created by some cyanobacteria blooms, and have become a global concern due to their potential to compromise animal and human health. The increasing occurrences of these harmful algal blooms and their toxins is attributed to heightened nutrient loading (excessive nutrients brought by runoff) and warmer water temperatures owing to climate change. Cyanotoxins pose a significant challenge for all municipalities that treat and distribute drinking water.
Ozone is considered the strongest oxidant that
can degrade these harmful compounds and is also the optimal drinking water treatment strategy for preventing human exposure to cyanotoxincontaminated water.
This research project aims to study the simultaneous oxidation and degradation of Microcystin-LR (MLR), Anatoxin-A (ANA), and Cylindrospermopsin (CYN) in the Detroit River. This will be done by using ozone and/or ozone-based AOP at various pH levels and temperatures, along with different doses of hydrogen peroxide. The goal is to ensure the production of safe, high-quality drinking water.
The Detroit River is a vital source of drinking water for the City of Windsor and the Municipalities of Tecumseh and LaSalle, serving a combined population of over 280,000.
The research findings from this project will provide useful information for water treatment systems in the Great Lakes region, whether they currently use ozone for drinking water treatment or are considering system upgrades to address the threat of cyanotoxins.
This project is led by Dr. Saad Jasim, President of SJ Environmental Consultants (Windsor) Inc., an expert in the field of ozone-based water treatment. He is also Adjunct Professor, University of Windsor, the Editorin-Chief of Ozone News and Past President of the International Ozone Association. Experiments will be conducted at the Civil and Environmental Engineering Department laboratories at the University of Windsor. This project represents a strong collaboration between industry, academia, government, and the private sector.
The project findings will provide government agencies with valuable information to reevaluate existing guidelines.
Dr. Saad Jasim, P.Eng.
New Presidents Nominated for IOA and EA3G
Dr. Renate Viebahn-Haensler has been nominated as the IOA International President (2026–27). Dr. Renate is the President of IOA-EA3G for 2024-2025. Serve as Scientific Consultant in Medical Ozone (Technology and Pharmacology). Board Member of the International Ozone Association IOA. Editorial board member of the scientific journal of the IOA: “Ozone: Science & Engineering”. Contributed many scientific publications on medical ozone applications. Executive Board Member of the “Medical Society for Ozone Application in Prevention and Therapy”
Dr. Cristian Carboni
Dr. Cristian Carboni has been nominated as EA3G President (2025–26). Dr. Cristian Carboni is the IOA-EA3G 20242025 President-elect. Serves as Manager of Innovative Disinfection Systems of Industrie De Nora S.p.A., Italy. He brings more than 25 years of experience in project management, research and technological innovation, author of numerous scientific publications particularly regarding ozone and its applications. Member of the scientific committee of Polo Agrifood, member of the Board of Directors of the International Ozone Association, Leader of the Water Europe Working Group Water and Public Health and board member of the Lombardy Energy Cleantech Cluster (LE2C).
Sidestream Injection & Pipeline Flash Reactor (PFR)...
• uniformly distributes ozone
• minimizes size & cost of ozone contacting system
Dr. Renate ViebahnHaensler
33rd Japan Ozone Association Annual Conference on Ozone Science & Technology, Kyoto, Japan, June 13–14, 2024
The 33rd Japan Ozone Association Annual Conference was held in Kyoto City, Japan at the Ryukoku University, on June 13–14, 2024.
Invited Presentations:
1 — “Food and Water Shaping the KYOTO”, Emeritus Professor ONO Yashiro, Kyoto Institute of Technology, Kyoto, Japan.
2 — “Characteristics and Future Prospects of Electrolytic Advanced Oxidation Processes”, Professor KISHIMOTO Naoyuki, Ryukoku University, Kyoto, Japan.
3 — “Sustainability will be Impossible without Change”, Dr. Saad Y. Jasim, Editor-in-Chief, Past PresidentInternational Ozone Association (2020-2022).
4 — “Case Studies make a Strong Case for Ozone Sidestream Injection”, Jim Lauria, Mazzei Injectors company, President Elect IOA-PAG (2026-2027).
Dr. Keisuke A Ikehata, Texas State University, Chair, Publication Committee of the IOA presented the topic: “Taste and Odor Compounds in Reclaimed Water: Occurrence and Removal by Advanced Water Purification Processes.”
The President of the Japan Ozone Association Dr. Atsushi Masuko, and Mr. Tetsuya (Ted) Tamura, Secretary General of Japan Ozone Association had a very warm welcome to Dr. Jasim, Dr. Ikehata and Mr. Lauria after their arrival
to participate in the 33rd Japan Ozone Association Conference. The Conference took place at the Ryukoku University, Fukakusa Campus. The conference was attended by 123 delegates including 9 students.
Presentations were delivered for the two days of the conference. Some of the presentations included:
• Removal of Residual Antibiotics in Actual Wastewaters using Ozone-based Advanced Oxidation Process: Evaluation and Prediction of Removal Performance
• Examination of Ozone-based Microalgae Domination Technology for the Realization of a New Wastewater Treatment Technology
• Removal of Antibiotic Resistant Bacteria and their Antibiotic Resistance Genes in Hospital Wastewater using Ozone
• Enhancement of Foulant Removal of Ceramic Membrane by Nano-bubble after Ozonation
• Combination of Amino Acids at Target Sites to the inactivation of SARS-CoV-2 (B.1.1.529) Spike Protein by Ozonated Water
• The Comparative evaluation on Removal Characteristics of Membrane Fouling Substances using Ozonated Water and Sodium Hypochlorite
• Evaluation of the Effects of Sludge Ozonation on the Removal of Antibiotic Resistant Bacteria (ARB) and Antibiotic Genes (ARGs) from Excess Sludge
• Decolorization of colored solutions in water purifications technology by water
In addition, there were many other presentations. Poster presentations included:
• Decomposition of Organophosphate Pesticides in Wastewater by using Advanced Oxidation Process
• Study on Efficient Ozone Water Generation Technology Using Fine-Bubbles
• Mineralization of Diclofenac by Advance Oxidation Processes combining Electrolysis and Ozone
The topic of Antibiotic Resistant Bacteria (ARB) and Antibiotic Resistance Genes (ARGs) had more than one presentation. One of the representations indicated that
anaerobic digestion of swage sludge is recognized that it cannot be effective in removing Antibiotic Resistance Bacteria (ARB) and Antibiotic Resistance Genes (ARGs) in the sewage sludge. The study objective was to quantitively evaluate the effect of ozone pretreatment, which is expected to promote anaerobic digestion and removal of both ARBs and ARGs. It was found that E. coli can be inactivated to below quantification limit by ozone consumption of 50-70 mg O3/gTS, while the ozone consumption should be increased to 95 mg O3/ gTS to increase the log removal rate of ARGs from 1.5 to 2. Another study was about fouling control in drinking water treatment with ceramic membrane tested to investigate the effect of Ultra Fine Bubble (UFB, same as nano-bubble) dispersed water in cleaning water during flushing (surface cleaning) after backwashing. Ozone and UFB were applied to fouling on ceramic membranes accumulated by surface water in a short labo test, and ozone and UFB were added to the backwash and flushing water, respectively. Although the dissolved ozone was effective for removing irreversible foulants, the UFB suspended flushing water was found to be additionally able to remove irreversible foulants.
Study was presented regarding the decolorization effects of a colored liquid is investigated using a water purification system that integrates water electrolysis and fuel cells for energy efficient ozone treatment. The primary goal was to evaluate the capability of the system to decompose chemical substances essential for water purification. A blank ink solution was processed with ozone bubbling. The clarity of the treated water was quantified using a luminance-based transparency index. The transparency index achieved value demonstrated the effectiveness of the treatment.
Potable water reuse is becoming increasingly popular in rapidly growing urban areas to secure water supply. However, tertiary effluent contains many taste and odor (T&O) compounds derived from sewage and its treatment. A presentation was delivered on the investigation of occurrence and removal of (T&O) compounds by ozone-biological filtration within a pilot advanced water purification system. Among the T&O compounds detected 2,3,6- tribromoanisole was found to be resistant.
Distinguished Service Prize: Dr. Hiroshi Tsuno
Technical Prize: Mitsubishi Electric Corporation, Mr. Yoshifumi Hayashi et al.
Article Prize for Encouragement: 1.Kanaza Institute of Technology, Mr. Tatsuya Kitayachi et al. 2. Mitsubishi Electric Corporation, Mr. Yokou Matsuura et al. 3. Tada Electric Corporation, Mrs. Mika Nakatouge et al.
Prize for Excellent Article: 1.Hanshin Water Supply Authority, Mr. Kyohei Uchigami et al. 2. Ryukoku University, Dr. Naoyuki Kishimoto et al.
Appreciation Letter: Sendai Umino-Mori Aquarium
Awards were presented during the second day of the conference. The annual General Meeting followed by the end of the conference.
Thank you to the organizers of the conference for their warm and generous welcome to all attendees, especially Mr. Tamura, Secretary General of Japan Ozone Association, for his tireless follow up for all conference details and attendees needs.
Congratulations to the Japan Ozone Association for organizing a successful and well-organized conference.
International Association for Great Lakes Research 67 Annual Conference
With over 800 delegates in attendance representing 18 countries and multiple First Nations and Tribal groups, the 67th Annual Conference on Great Lakes Research provided an opportunity to welcome the global Great Lakes community to Windsor. This was the 5th time this event has been hosted by the University of
Windsor which reinforces the rich history of Great Lakes research at the University where Healthy Great Lakes have long been included as one of our pillars of strategic research activity.
Dr. Saad Jasim, Editorin-Chief, Ozone News, attended the conference which was held in Windsor, ON, Canada on May 20-24. Recognized as one of Canada’s most diverse and multicultural communities, Windsor was developed on land that is the traditional
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Dr. Saad Jasim with a group of scientists from Africa
territory of the Anishnaabeg people of the Three Fires Confederacy of First Nations (Ojibwe, Potawatomi, and Odawa). They refer to this unique location along the Detroit River, or Waawiyaataanong Ziibi, as “where the river bends.” 2024. The conference was hosted by the University of Windsor.
The program included four days of scientific sessions and speakers centered around the theme Shared Lakes: One Water, One Health. This focus offered a unique opportunity for both the science and local communities to share about the health of the Great Lakes and will highlight the intersections between environmental and public health in the context of large lakes, often shared across borders, and the communities impacted by them.
Conference sessions included topics such as; Advances in Large Lakes Science, Technologies & Innovations, Advances in Wetland Science, Cyanotoxins and Off Flavors in Freshwater: Synthesis Mechanism to Toxicology Assessment, Aquatic Invasive Species Research, PFAS Loading to and Discharges from Water Treatment Plants, and many other topics.
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Dr. Saad Jasim with Dr. Mike Macky, University of Windsor, Great Lakes Institute for Environmental Research-Site Chair
Middle East and North Africa Ozone Generator Market
Report by: MarkNtel Advisors
Middle East and North Africa Ozone Generator Market Driver: Burgeoning Water Scarcity in the Region
The significant stress towards rising water scarcity in the region has displayed a positive influence on the Middle East and North Africa Ozone Generator market as the water scarcity induced the demand for water and wastewater treatment facilities in the region. With the growing population and rapid industrialization, the water demand is surging at a dynamic rate. Thus, this has influenced the governments of the UAE, Saudi Arabia, Qatar, Egypt, etc., to supplement their spending on the establishment of various water desalination plants and wastewater treatment plants as well as for the
expansion of numerous wastewater and sewage treatment capacities. For instance:
• In 2021, the government of Egypt planned to invest approximately USD2.5 billion to establish new desalination plants over the next five years to avoid future water stress. Hence, the surging water scarcity in the region has augmented the application of ozone generators for treating wastewater for reuse purposes in the region, therefore positively impacting the market outlook in the following years.
Middle East and North Africa Ozone Generator Market Opportunity: Surging Demand for Sustainable Products in Chemical Industry
Developments in the chemical industry, especially in sectors such as paints and coatings, agrochemicals, and specialty chemicals, would further attribute the demand for ozone generators in the region owing to the growing emphasis on sustainability in these industries. In 2021, at the 4th Gulf Petrochemicals and Chemicals Association (GPCA)
Responsible Care Conference, the GCC chemical industry have reiterated their commitment to advancing sustainability, developing nature-positive solutions, and contributing to climate action. Ozone generators are considered a more environmentally friendly alternative to certain chemicalbased purification methods which would push the demand for ozone generators.
Additionally, the production and establishment of chemical industries have also been growing due to the feedstock advantages and the strategic location of the Middle Eastern and North African countries. The abundant feed stock is attracting various major chemical companies such as Chevron, Bodo Möller Chemie, etc., to establish their chemical production plants in the region which would further drive the market in coming years.
Middle East and North Africa Ozone Generator Market Challenge: Presence of Alternatives Technologies to Restrict the Market Growth in the Region
The presence of alternative technologies for air and water purification, such as activated carbon filters, UV sterilization, and chemical disinfectants has been posing a threat for the growth of the Middle East and North Africa Ozone Generator Market. These technologies are present in the market for long time and are perceived as more familiar and reliable by consumers. In addition, the cost-effectiveness of competing technologies, established supply chains, and applicationspecific requirements has also been restricting the adoption of ozone generators in the market.
Middle East and North Africa Ozone Generator Market Trend: Surging Applications of Ozone Generators as Air Purifiers
Ozone generators have become more popular as air purifiers to combat airborne toxins, smells, and germs. As a result of the rising number of airborne diseases in the area, ozone has been efficient in removing bacteria, fungi, germs, odors, and other pollutants, destroying the cell wall at the molecular level. In this process, the pollutant is removed by converting ozone back to oxygen. Thus, these generators have been used as air purifiers in residential and commercial premises. Consequently, their market demand is anticipated to grow extensively during the forecast period due to the surging expansion of residential and commercial buildings.
The Middle East and North Africa Ozone Generator Market (2023-28): Segmentation Analysis
The Middle East and Africa Ozone Generator Market study of MarkNtel Advisors evaluates and highlights the major trends and influencing factors in each segment and includes predictions for the period 2023–2028 at the national levels. Based on the analysis, the market has been further classified as:
Based on Technology:
• Corona Discharge
• Ultraviolet light
• Electrolysis
• Radiochemical
Corona Discharge held the highest share of the Middle East and North Africa Ozone Generator Market over the historical period owing to its large-scale application in the commercial and industrial sectors. The cost-effective technology and low maintenance expense in comparison to other technologies such as UV light and radiochemical has positively impacted the demand for corona discharge technology.
Thus, with the increasing urbanization and pollution, the market demand for corona discharge ozone generators for water and air treatment is estimated to expand in the forecast years. Besides, the electrolysis market is emerging at a rapid pace as it produces ozone from water rather than gaseous air or oxygen. These types of ozone generators are commonly employed in the pharmaceutical, cosmetic, and semiconductor sectors, leading to the growth in the Middle East and North Africa Ozone Generator Market.
Based on Application:
• Water Purification
• Air Purification
• Food Processing
• Organic Synthesis
• Aquaculture
• Medicine and Aesthetics
• Others (Surface Treatment, Swimming Pool)
Water Purification held a considerable market share, as it is widely used for eliminating bacteria, parasites, and viruses from water that are utilized in various industries. It also includes the application of ozone generator for water disinfection, municipal wastewater treatment, decolorization in water and wastewater treatment plants, bottling plants, etc., contributing to the market growth. Furthermore, the surging government spending of the regional countries on wastewater treatment plants due to growing concerns about water scarcity is expected to push the demand for ozone generators in the coming years. The UAE, Saudi Arabia, and Qatar are some countries heavily investing in water treatment plants. Hence, due to the burgeoning investment by the country’s government in treating wastewater, the market demand for ozone generators would grow substantially in the region.
33rd Japan Ozone Association Annual Conference on Ozone Science & Technology, Kyoto, Japan June 13-14, 2024
Highlights:
Location — Las Vegas, Nevada
Scope of Project:
• Engineering & Project Management to support design
• On-site support & startup
• (2) 13,000 gal LOX storage tanks
• (2) Ambient Air Vaporizers
• Vaporizer Switching Manifold
• Downstream Pressure Manifold
Flow spec: 30,000 SCFH, 24 hour duty
• 316/L stainless steel piping
• 316/L stainless steel valving
• Project Completion: 2013
System Configuration:
Application:
A water treatment plant located in Nevada used to treat the water for Clark County. The oxygen system was used to provide gaseous oxygen to the ozone generators. The design of the system was to accommodate a future, build-out for tanks and vaporizers.
Project Background:
The project was a public bid that had Chart Industries in the specification. The general contractor approached Chart with the specifications. It was recommended by Chart that we procure and fabricate all the large bore piping to reduce field installation. By doing this, Chart took the responsibility of the vendor coordination, reducing the general contractors resource requirements.
Significant Accomplishments:
Providing equipment per the engineering specifications for capacity, material requirement, cleanliness, flow requirements and seismic per Nevada seismic requirements. Taking the responsibility of fabricating the GOX Pressure Manifold greatly reduced the amount of field installation required for this project.
Chart’s two horizontal 13,000 gal vessels with brass/bronze plumbing. Vessels included tank mounted pressure & level transmitters. Chart also included ambient vaporizers rated for 30,000 SCFH each. The GOX Pressure Manifold was 8” SCH40 SMLS piping with butterfly valves mounted to electric actuators as well as temperature transmitters. The GOX manifold included a 316 stainless regulator and was skidded to a stand.
“Just wanted to drop a note and let you know that Chart has been very helpful to McCarthy team at AWT. When my Mechanical Manager was out of town, Chart was more than happy to jump in and help out. I just want you to know that your efforts are recognized by McCarthy. We need more vendors and subcontractors that want to and are willing to help like Chart.“
Brandon Moon
McCarthy Building Companies, Inc.
Ozone in Medicine. Ozone as Redox Bioregulator
III. Virus Diseases
Renate Viebahn-Haensler and Olga Sonia León Fernández
Key words: redox medicine, redox bioregulation, virus diseases, ozone therapy
Abstract: Viral diseases with high oxidative stress, antioxidant deficiency, an unbalanced redox system and immune dysbalance as accompanying symptoms are the domain of systemic ozone application in a complementary therapeutical concept. However, less in the acute state -in which the biological response of the organism is running at full speed- than in the chronic state; as discussed here in the context of Hepatitis C and COVID-19. Restoration of the redox balance is one of the essential results of the low-dose ozone concept.
Introduction
as discussed in part II of these contributions in Ozone News no 2, 2024. An ozone peroxide RCH(OH)OOH, formed through the reaction of O3 with unsaturated acids (having isolated double bonds) in the cell membrane seems to be the active compound: it is immediately reduced by Glutathione GSH and thus starting redox regulation and immunomodulation.
Viruses are an opportunistic species simply waiting for a host with an immune deficit; on the other hand, we live in coevolution and coexistence with viruses.
In fact, they are the oldest biological entity on our planet; we host approximately 1015 viruses in our body (per 1013 human cells) and around 50 % of the DNA is of viral origin with a promoter function in evolution (Moelling 2016: Viruses, more friends than foes).
Hepatitis C
As a human pathogenic virus, hepatitis C, for example, an RNA virus similar to the corona virus, attacks the liver and immediately triggers the body’s own immune response.
HCV-specific CD4+ and CD8+ T cells are activated. and plasma cells produce neutralizing antibodies. After viral clearance, memory cells are maintained. The patient is healthy.
At the turn of the 19th/20th century, sewage and drinking water were recognized as one of the major epidemic causes of typhoid, cholera etc., and appropriate water treatment plants were built. In most countries, this nightmare soon became a thing of the past though, unfortunately, not everywhere in the world even up to the present. As one of the most potent disinfectants, ozone is used in installations making use of its high oxidation potential in the degradation of toxic substances; its germicidal, bactericidal, and antiviral properties guarantee perfect drinking water, the most important basic food. In order to utilize the antiviral and microbicidal effect of ozone, an aqueous environment and direct contact between ozone and microbes is required.
These conditions cannot be transferred to the biological system; when ozone is applied in animals and humans, bacteria, viruses and microorganisms can never be directly attacked. Path length and half-life of the O3molecule are very short in all forms of application. This means that we are dealing with an indirect mechanism
However, in approximately 50 % of patients, the immune system is weakened, the T cells are exhausted and deleted, and chronic hepatitis C develops.
While all biological defense mechanisms are running at full speed during an acute infection , chronic processes are accompanied by immune deficiency and high oxidative stress.
These are the domain of medical ozone, rather than acute events. The redox balance is restored and the immune system is modulated.
The liver is the organ that responds best and fastest to systemic low-dose ozone applications and hepatitis is one of the classic indications for medical ozone; here, biotransformation (detoxification) is activated and regenerated, measurable by changes in specific liver parameters.
Direct antiviral therapy concepts with a high success rate have recently been developed for virus-related diseases such as hepatitis B and C. Ozone can be used as a supplement or if this antiviral therapy is not tolerated or fails.
Olga Sonia León Fernández
Renate ViebahnHaensler
Despite everything, hepatitis C is still a worldwide problem, it usually occurs as a co-infection (HIV-AIDS, blood contact, sharing syringes in drug abuse, piercing …) and is even endemic in some countries, such as genotype 4 hepatitis C in Egypt. Several clinical studies have been published: we would here like to focus on Mawsouf and colleagues from Egypt demonstrating the effectivity of systemic ozone application in 60 genotype 4 patients by measuring the viral load by PCR (polymerase chain reaction): After two months, the viral load had decreased in 91.67% of cases, with negative PCR results in 20% of patients; after a total of 6 months of treatment, 95% of patients had benefited from a decreasing viral load, and 36.67% were virus-negative.
COVID-19
In times of corona pandemics these studies are of special interest: Both viruses are RNA-viruses and similar results can be expected for the chronic corona disease COVID, the post or long COVID syndrome and post vaccination syndrome, all of them accompanied by immune disbalance
(cytokine storm: high IL-6, IL-1 and others) and high oxidative stress. Here we selected malondialdehyde MDA as oxidative stress marker and GSH (glutathione active, reduced form) as antioxidant marker to emphasize restoration of the redox balance by low-dose medical ozone application.
A randomized
controlled clinical study including 32 patients: Post-COVID conditions (comparable to the post-vaccine syndrome) with long-lasting high oxidative stress, low antioxidant capacity, proinflammatory cytokines in excess, and subsequently an imbalanced glutathione system are a typical indication for a low-dose ozone application. The pharmacological mechanisms accounting for the effectiveness against COVID-19 have been summarized in León Fernández et al. (2021). According to an open label, controlled clinical study (Gil-Del-Valle et al. 2020), the convalescence phase is shortened and the clinical symptoms are significantly improved. Here again, we focused on the highly sensitive parameter GSH to emphasize the redox-regulating effect of ozone.
Study Design
The study comprised 32 patients, already 3 weeks virusnegative: the PCR-negative (polymerase chain reaction) patients remained hospitalized for a further 4 weeks, and were treated as follows: group I (n = 16), received a standardized vitamin treatment and group II, i.e. the ozone group (n = 16) received additional ozone treatment in the form of rectal insufflation.
Treatment frequency was twice per day with 12 hours in between for five days per week, in a schedule comprising 40 treatments. The concentrations started with 20 μg/mL during the first week, with an increment of 5 μg/mL
Figure 1. Chronic hepatitis C patients (n=60). a. PCR decline and b. PCR negative patients after 2 and after 6 months of systemic ozone application.
Figure 2. Post COVID patients (n=32). Receiving vitamins or vitamins+ozone for 4 weeks in comparison to healthy controls. a. GSH repair marker: 50 % increase in the ozone group. b. Oxidative stress marker MDA decreases accordingly in the ozone group (Gil-DelValle et al. 2020, Viebahn-Haensler, León Fernández 2024).
per week up to 35 μg/mL in week 4, with the volume of the insufflated ozone/oxygen mixture being increased from 150 mL over 3 weeks (corresponding to 3,000 μg O3 per treatment) up to 200 mL over the last 5 days (7,000 μg O3).
Results
Compared to the healthy control group (n =20), all post COVID patients show dramatically decreased glutathione GSH and thus a decreased antioxidant capacity. Accordingly, these patients are susceptible to infections (viruses, bacteria) and other comorbidities. Four weeks of intensive treatment with a standardized vitamin complex brought only minor improvements, whereas, in the ozone group (vitamin + ozone), a 50% increase in the active form of glutathione GSH led to a substantial improvement in antioxidant capacity and restoration of the balance (2 GSH GSSG).
An overall improvement (here not shown) – clinical parameters included – show that 40% of patients benefited from vitamin administration and 90% from combined application of a vitamin complex plus ozone: this is correspondingly reflected by an increase in GSH and a decrease in MDA as oxidative stress parameter p < 0.05 (Figure 2).
Conclusion
As part of a complementary treatment concept in chronic virus diseases or post-viral syndrome, low-dose ozone application shortens the convalescence and recovery phases by restoring redox balance as a result of antioxidant upregulation, a decrease in oxidative stress and modulation of the immune system.
References
Gil-del-Valle, L., López-Fernández, O.E., Sánchez- Márquez, J.A. Zamora-Rodríguez, Z. Carballo-Reyes. A.L. Fernández-García, L.A. Delgado-Guerra, M.M. Fonseca-Gil-del-Valle, L. LópezFernández, O.E. Sánchez- Márquez, J.A. Zamora-Rodríguez, Z. Carballo-Reyes. A.L. Fernández-García, L.A. Delgado-Guerra, M.M. Fonseca-Betancourt, F. Miyares-Díaz, E. Piloto Orraca, Y. Bermudez-Alfonso Y. Hdez Glez-Abreu, M.C. Mendoza-Castaño, S. De Armas-Aguila, Y.R. (2020) Amelioration of Symptoms and Oxidative Stress in Hospitalized Convalescent Post SARS-COV-2 Patients Treated with Rectal Ozone Therapy and Nutritional Supplementation. IJMPR. 4: 94-107. https://ijmpronline.com/ admin/assets/article_issue/1609153795.pdf
León Fernández, O.S. Takon Oru, G. Vieahn Haensler, R. López Cabreja, G. Serrano Espinosa, I. Polo Vega, J.C. García Fernández, E. (2021) Medical Ozone: The Pharmacological Mechanisms Accounting for its Effectiveness against COVID-19/SARS-COV-2, Clin Res & Rev. 5:3, 1-10. https://scivisionpub.com/pdfs/ medical-ozone-the-pharmacological-mechanisms-accountingfor-its-effectiveness-against-covid19sarscov2-1581.pdf
Mawsouf M.N. Tanbouli,T. Viebahn-Haensler, R. (2012) Ozone Therapy in Patients with Viral Hepatitis C -Ten Years Experience, Ozone: Sci Eng 34: 425-431 https://doi.org/10.1080/01919512.20 12.720161
Moelling K. (2020) Viruses, More Friends than Foes.. Vb World Edition, ISBN: 978-9811224744
Viebahn-Haensler, R. León Fernández, O. (2024) Ozone in Medicine. The Low-Dose Ozone Concept. The RedoxBioregulatory Effect as Prominent Biochemical Mechanism and the Role of Glutathione, Ozone: Science & Engineering, 46:3, 267-279, DOI: 10.1080/01919512.2023.2291756
Welcome, New Members
JAPAN
IOA-PAG NEW MEMBERS
JUNE 2024
BOTSWANA
Kgosiyareng Isaac Tumelo dulletventures@gmail. com
Dullet Ventures PO Box 20638 Gaborone Southeast 00268
CANADA
Michael Doran mike@sublimes.ca
Sublime Environmental 1325 Hilliard Street
Peterborough, ON K9H5S3
Jon Simaitis jon.simaitis@halton.ca
Halton Region 1151 Bronte Road Oakville, ON L6M 3L1
Kai Yamabe
yamabe.kai.37i@st.kyoto-u. ac.jp
Kyoto university 6-1, Kamimachi5 Kochi, Kochi 7800901
SPAIN
Isabella Sieiro isabellasieiro@gmail.com
Universidad Politecnica de Valencia Camí de Vera, s/n, Algirós 46022 Valencia
URUGUAY
Alvaro Irigoyen irigoyen@netgate.com.uy Ing. Alvaro Irigoyen Buenos Aires 618-501 Montevideo 11100
USA
Ronnie Box rbox@springfieldmo.gov
City of Springfield 3301 S State Hwy FF Springfield, MO 65807
Joe Cannavino joe@atavisplasma.com
Atavis Plasma Systems 2590 El Camino Real Atascadero, CA 93422
Jack Davis jdavis@springfieldmo.gov
City of Springfield, Missouri 3301 South State Highway FF Springfield, MO 65807
Lance Kovash lkovash@fargond.gov
City of Fargo 435 14th Ave S Fargo, ND 58103
Mason Mannie mmannie@fargond.gov
City of Fargo 435 14th Ave S Fargo, ND 58103
Clover Stout bcstout1@msn.com
Veolia WTS 11230 N River Road Payette, ID 83661
Craig Thompson cthompson@westyost.com West Yost 1001 Galaxy Way, Ste 310 Concord, MA 95420
Maddie Zimmerman maddie.zimmerman@ denora.com
Bundle (B) - Corporate
Alonso Hurtado alonso.hurtado@toronto.ca
Bundle (A) - Operations
Sarah Wilson sarah.a.wilson@toronto.ca
Bundle (A) - Operations
IOA—International Ozone Association EA3G2024 – Europe, Africa, Asia, Australasia Group International Conference
VILA NOVA DE GAIA
(PORTO)
PORTUGAL, PORTUGAL • WORLD OF WINE NOV. 27–29 2024
Ozone and Advanced Oxidation
Science, technologies and applications for a better world.
EA3G2024 renews with a long series of successful conferences which were organised to provide an international forum for all concerned including fundamental, engineering and applied aspects of oxidation techniques involving ozone and advanced oxidation systems.
SCOPE & OBJECTIVES: Population growth associated with increases in urbanization, in consumption, in industrialization, in pollution emissions already lead to shortages of water, food, raw materials and globally to climate change. However, climate and earth-resilient development is still possible by devoting greater efforts to certain key sectors: reduction of emissions to air, better resources management and preservation of the natural ecosystems. This involves particularly the implementation of advanced technologies. EA3G2024 event thus aims to present an overview of the current state of knowledge and the latest advances regarding the use of Ozone and Advanced Oxidation to provide solutions face to these challenges.
Topics of interest relevant to the Conference theme include but are not limited to the combinations of:
• Reaction mechanisms, kinetics, by-products
• Ozonation
• Advanced oxidation
• Disinfection
• Ozonolysis
• Hydraulics, hydrodynamics, mass transfer, contactors
• Modeling, validation, system design
• Analytical methods
• Agri-Food production, safety and quality
• Waste, Air and Soil treatment
• Wash water, process water
• Wastewater treatment, recycling and reuse
• Ecosystem preservation
• Industrial applications
• In-field full scale applications
• Product / Process R&D
• Hybrid ozone processes
• Innovative applications
• Regulations
REGISTRATION
• Registration will be available early July 2024.
• The registration fee will cover scientific and technical sessions, book of abstracts, electronic proceedings, lunches, coffee breaks, exhibition and technical tour.
LANGUAGE AND CONFERENCE VENUE
• The official language will be English.
WINTER SCHOOL
PROGRAMME:
• Scientific and technical sessions with oral and poster presentations, discussions
• Networking opportunities and Exhibition of ozone related products and services
• Technical visit of ozone plant(s)
• Conference dinner for delegates and their guests
CALL FOR PAPERS — PUBLICATIONS:
• Researchers, scientists, and professionals are invited to propose oral or poster presentations related to the theme topics.
• Authors should submit an extended abstract in English of two pages (with tables and figures) (dates to be announced soon).
• The Programme Committee will notify authors about the acceptance of their papers for oral or poster presentation (dates to be announced soon).
• During the conference, the Programme Committee will select and award a prize to the best paper presented by a PhD student. All accepted papers will be printed in the conference proceedings and handed out to participants at registration. After the conference, the editors of the IOA journal “Ozone: Science & Engineering” will make a final selection for a potential publication as peer-reviewed article.
ASK EXHIBITION AND SPONSORING OPTIONS:
• The exclusiveness of the conference theme allows exhibitors and sponsors an ideal opportunity to meet a unique and targeted audience.
• For detailed information on sponsoring options, please contact the IOA-EA3G secretariat to make the most of this event.
• A Winter School on “Contaminants of Emerging Concern and Disinfection By Products: Occurrence, Impact and Elimination,” will be held in the World of Wine, from 25 to 26th November, just before the EA3G2024.
• Winter School participants will have a reduced fee in EA3G2024 conference, equivalent to IOA members.
• The Winter School will be organized by the Faculty of Engineering of the University of Porto, University of Coimbra, and Sociedade Portuguesa de Inovação (SPI), under the scope of the projects BlueWWater (INTERREG-POCTEP) and H2OforAll (Horizonte Europe), with the support of IOA.
• The Winter School program will include: i) Floor to PhD Students: section specifically devoted to PhD students where they will have the chance to introduce themselves and their work, through a short oral presentation and/or a poster communication, as well as to meet experts from the School; ii) Lectures on complementary skills: European legislation on drinking water, urban wastewater treatment, and wastewater reuse; Advanced analytical methods for CECs and DBPs, microplastics, ARB, ARGs; Risk assessment; Advanced treatment technologies (Ozonation, Advanced Oxidation Processes, Adsorption, Membrane Filtration); Life Cycle Assessment; Life Cycle Costing; Simulation; iii) Workshop on Wastewater Treatment and Reuse; iv) Workshop on Disinfection by Products (DBPs).
• PhD students are invited to submit an extended abstract in English of two pages (with tables and figures) (dates to be announced soon).
• The Programme Committee will notify authors about the acceptance of their papers for short-oral or poster presentation (dates to be announced soon).
International Ozone Association—
Pan American Group (IOA-PAG) 2024 Conference
AUG. 26–29 2024
Palms Casino Resort, Las Vegas, Nevada
Benefits of Attending Include:
• Two days of technical presentations by Ozone experts
• Round-table discussion session hosted by industry leaders
• Workshop for those who are new to Ozone technology
• Exhibit hall with leading manufacturers of Ozone equipment
• Networking opportunities to learn more about Ozone applications
• Technical tour to learn more about other Ozone installations
Technical Program
The Technical Program will feature two days of technical presentations on subjects, such as drinking water treatment, water reuse, industrial applications, etc. Presenting authors may have their work considered for publication in Ozone Science & Engineering (OS&E), the official journal of the International Ozone Association. All conference attendees will receive a complimentary copy of the conference proceedings.
The IOA-PAG Conference will showcase leadingedge technologies for municipal water, municipal wastewater and industrial process applications with special attention to both Ozone and AOP technologies.
Looking Back at 2023 Kansas City Conference
https://ioa-pag.org/page-18204
Conference Registration
Conference
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Early-Bird Rate
Deadline: June 30, 2024
About the Technical Tour
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Technical Tour Waiver
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More information will be added as it becomes available. If you have any questions, please reach out to support@ioa-pag.org or call 916-441-0629.
Utility Registration Bundle!
Book your stay under IOA-PAG’s exclusive room block rate of $99/night by Wednesday, July 31!
Reserve Discounted Room
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About the Palms Casino Resort
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Utilities and other end users of ozone registering three conference attendees will receive a complimentary registration for a fourth attendee to the 2024 IOA-PAG Conference. (Buy 3 Get 1 Free!)
In order to receive the discount, please complete the regular registration process for your first 3 attendees and send their names to IOA Support (support@ioa-pag.org). Once registration has been confirmed, you will receive a coupon code to sign up your complimentary (4th) registration using Registration Category (K).
Buy 3 Get 1 !
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