SDS-PAGE G e l M atrices for Protein Electrophoresis U l t r a - Pure Buffers V i s u a l ization Products P r o t e i n Standards W e s t e rn Blotting
There’s an Art to this Science REAGENT SYSTEMS FOR LIFE SCIENCE DISCOVERY
Protein Electrophoresis Products Gel Solutions 30% Acrylamide
®
ProtoGel 37.5:1 30% or 40%, 37.5:1 Acrylamide to Bisacrylamide FOR ROUTINE SDS-PAGE AND FOR HIGHEST RESOLUTION SEPARATION OF MEDIUM TO LARGE PROTEINS (> 60KD) TM
0.8% Bisacrylamide
AccuGel 29:1
No Acrylic Acid
30% or 40%, 29:1 Acrylamide to Bisacrylamide FOR ROUTINE SDS-PAGE AND FOR HIGHEST RESOLUTION SEPARATION OF SMALL
Every lot of ProtoGel and AccuGel 29:1 is HPLC certified.
TO MEDIUM PROTEINS (< 20KD) ● Consistently Crystal Clear Gels, Zero Fluorescence ● Stabilized for Two Year Shelf Life ● 0.2 Micron Filtration / 18 MegOhm Water ● Virtually Zero Acrylic Acid Content ProtoGel 37.5:1 30% 450ml 1 liter (1-3) 1 liter (4 +)
EC-890
AccuGel 29:1 30% 450ml 1 liter (1-3) 1 liter (4 +)
EC-851
ProtoGel 37.5:1 40% 450ml 1 liter (1-3) 1 liter (4 +)
EC-891
AccuGel 29:1 40% 450ml 1 liter (1-3) 1 liter (4 +)
EC-852
ProtoGel Quick-Cast 12% Ready-to-Use SDS-PAGE Gel Solution
New!
● Cast a Gel in 20 Minutes ● Ideal for Western Blotting Applications ● Measure, Add Initiators, & Pour Gel - That’s It!
ProtoGel Quick-Cast 12% 100ml 450ml
Pre-Cast Convenience without the Pre-Cast Price
EC-895
Ultra-Pure Buffers ProtoGel Resolving Buffer (4X) EC-892 450 ml 1 Liter (1-3) 1 Liter (4+)
Tris-Glycine-SDS PAGE Buffer (10X) EC-870 1 Liter 4 Liter (1-3) 4 Liter (4+)
ProtoGel Stacking Buffer (4X) EC-893 200 ml
Tris-Tricine-SDS PAGE Buffer (10X) EC-869 1 Liter
Protein Loading Buffer Blue (2X) EC-886 10 x 1 ml
CONTACT NATIONAL DIAGNOSTICS
USA: 1-800-526-3867
EUROPE: 44(0) 1482 646020
www.nationaldiagnostics.com
Gel Stains ProtoBlue Safe TM
Wash gel 3 times for 5 minutes with deionized water on an orbital shaker.
Eco-Friendly, Ultra Sensitive Colloidal Coomassie G-250 Stain
(This wash step is optional. A longer incubation in stain solution compensates for bypassing this step.)
5 ng BSA
● The Best Performing Colloidal Stain ● Less Cost than Regular Coomassie ● Nonhazardous Disposal
Add 1 part ethanol to 9 parts staining solution while stirring. Standard denatured ethanol is fine.*
Samples separated on a 12% standard Laemmli gel, stained overnight with ProtoBlue Safe.
● Fast Protocol ● No Methanol or HOAc
Add enough staining solution to completely cover the gel (20 - 50 ml).
With unmatched advantages in sensitivity, safety and waste disposal, ProtoBlue Safe represents the best all around stain for protein gels. In addition, ProtoBlue Safe is less costly than regular Coomassie staining, which requires large volumes of acetic acid and methanol for destaining. ProtoBlue Safe has the ability to detect less than 5ng denatured BSA, and contains no methanol, acetic acid, or other hazardous components. ProtoBlue Safe 1 liter 4 liter
Bands containing more than 1µg of protein will be detected within 15 minutes. For full sensitivity (up to 5ng), incubate the gel in stain for at least 5 hours.
EC-722
*Adding the ethanol immediately before staining greatly improves the sensitivity, reproducibly and shelf life of ProtoBlue Safe as compared to formulations where the ethanol is added at the point of manufacture.
TM
Sterling Rapid Silver Stain
Insite System
● Easy System with Fewer Reagents
● Staining Occurs During the Run
● Fix, Wash, and Stain in One Hour
● 10ng Sensitivity in 30 Minutes
TM
In-GEL Fluorescent Stain
Fast, High Sensitivity (<5ng) Silver Staining
● Ideal Prep for MALDI Mass Spec Sterling Rapid Silver Stain 1 kit (1-3) 1 kit (4 +)
SDS-PAGE mini-gel prepared following the Insite System protocol.
EC-720 Insite System 1 kit
EC-759
Protein Markers kDa
ProtoMarkers
TM
kDa
Broad Range Prestained Protein Standards ● 17kD to 190kD Range ● Red Protein Band Included for
Easy Orientation
● High Intensity Labeling
190 86 70 47 29 22
ProtoMetrics
TM
Engineered Unstained Protein Standards ● Engineered for Exact Mobilities ● 10, 15, 25 35, 50, 75, 100,
150, and 225 kD ● High Intensity 50kD Reference Band
17
ProtoMarkers 500 µl
225 150 100 75 *50 35 25 15 10 Gel stained with Coomassie blue.
EC-898 ProtoMetrics 500 µl
EC-899
Protein Cleanup and Concentration Sample Preparation ND Protein Precipitation Kit
New!
● Rapid Recovery and Concentration of Proteins from
Dilute Solutions
● Better than 99% Recovery of All Proteins from
Complex Mixtures
● Compatible with 2D Electrophoresis and IEP ● Precipitates as Little as 100ng of BSA @ 0.25µg/ml National Diagnostics’ Protein Precipitation kit makes the cleanup and concentration of proteins from dilute solutions fast, easy and reliable. A unique proprietary system brings down >99% of all proteins, even complex mixtures in dilute solution. Interfering salts and surfactants are left behind in the supernatant. Precipitated proteins are further purified with a rapid and gentle acetonitrile, acetone, or 70% ethanol wash, allowing the concentrated proteins to be recovered in a small volume of whatever buffer is optimal for the next procedure. ND Protein Precipitation Kit 1 Kit - Precipitates 50ml
EC-888
Protein Loading Buffer Blue (2X) National Diagnostics’ Protein Loading Buffer Blue (2X) is a readyto-use buffer solution for the preparation of protein samples to be separated in SDS-PAGE. Loading Buffer Blue (2X) contains Bromophenol Blue as a tracking dye to monitor the sample during the electrophoretic run. Loading Buffer Blue (2X) contains 0.5M Tris-HCl (pH 6.8), 4.4% (w/v) SDS, 20% (v/v) glycerol, 2% (v/v) 2-mercaptoethanol, and bromophenol blue in distilled/ deionized water.
Protein Loading Buffer Blue (2X) 10 x 1ml
EC-886
Western Blotting HRP Visualization and Ultra-Pure Blotting Buffers TM
TM
ProtoBlock System
HRPL Kit
● Enhances Light Production from
HRP Catalyzed Luminol Breakdown
● Rapid, Sensitive, Non-Isotopic Detection ● Reduced Background ● Bulk Size Provides Excellent Savings vs. the Competition National Diagnostics’ HRPL Kit is a nonradioactive chemiluminescence based method for the detection of horseradish peroxidase. The HRPL Kit causes light to be produced when any molecule labeled with horseradish peroxidase is exposed to the reagents in the kit. Light output peaks approximately 2 minutes after introduction of the reagent, and lasts for more than 1 hour.
● Protein Blocking Solution for Immunoassays ● Eliminates Endogenous Background ProtoBlock System 1 system
CL-252
Blotting Buffers Tris-Glycine Electroblotting Buffer (10X)
EC-880
HRPL Kit CL-205 240ml kit (1920 cm2 membrane) 900ml kit (7200 cm2 membrane)
1 Liter 4 Liter (1-3) 4 Liter (4+)
PBS (10X) Phosphate Buffered Saline
CL-253
450 ml 1 Liter 4 Liters
REAGENT SYSTEMS FOR LIFE SCIENCE DISCOVERY © 2007 National Diagnostics EC2007_01 CONTACT NATIONAL DIAGNOSTICS
USA: 1-8OO-526-3867
EUROPE: 44 (O) 1482 646O2O
WWW.NATIONALDIAGNOSTICS.COM