Biotechnology

Electrophoresis and DNA Fragment Length Determination

In this activity students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes. Students will not only learn about the process of DNA electrophoresis but learn the techniques associated with the process, such as setting up an agarose gel, loading DNA samples in the agarose gel, and staining the gel in order to visualize the DNA bands. They will also learn how to determine the sizes of unknown DNA fragments after examining their results. The kit includes specially-treated DNA samples that do not require refrigeration or freezing, prepared agarose that may simply be melted in a hot water bath or microwave, TBE electrophoresis buffer, and DNA stain. There is enough DNA to run 10 gels.

Kit Contains: 200mL 0.8% Agarose 500mL 5X Tris Borate ETA Buffer, 60mL 20X DNA Stain LAMBDA DNA ECORI DIGEST With Loading Dye LAMBDA DNA HINDIII DIGEST With Loading Dye LAMBDA DNA ECORI/HIND III DIGEST With Loading Dye

DOT Info: Non-regulated

IS3300 $101.97

DNA Fingerprinting Electrophoresis Lab Activity

DNA fingerprinting is one of the strongest forms of evidence in forensic investigations. In this activity students will perform agarose gel electrophoresis on four DNA samples to determine if DNA from suspects matches that of DNA found at a crime scene. Students will also learn about restriction enzymes and the importance of these enzymes in the DNA fingerprinting process. The kit contains enough materials for 8 groups and includes a Teacher’s Guide and Student Study copymasters.

Kit Includes: 200mL Prepared Agarose 0.8% Solution 500mL Tris-Borate-EDTA Buffer 5X 60mL DNA Stain 20X 10μg Crime Scene DNA 10μg Victim DNA 10μg Suspect #1 DNA 10μg Suspect #2 DNA

DOT Info: Non-regulated

IS3301 $101.97

DNA Paternity Testing Electrophoresis Lab Activity

While over 99% of DNA is the same among all humans, the remaining part is unique to every individual (with the exception of identical twins). These differences are hereditary, with parts of the unique sequence coming from each parent. By using DNA fingerprinting, or DNA profiling, a child’s paternal relationship can be tested by comparing the child’s DNA profile to the profile of the mother and possible father to determine if an individual is the father or not. In this activity students will use electrophoresis to separate DNA samples of a child, mother, and two potential fathers to determine if either father is a possible paternal match. The kit contains enough materials for 8 groups of students and includes a Teacher’s Guide and Student Study Guide copymasters.

Kit Includes: 200mL Prepared Agarose 0.8% Solution 60mL DNA Stain 20X 10μg Child’s DNA Sample 10μg Possible Father #2 DNA Sample 500mL Tris Borate EDTA Buffer 5X 10μg Mother’s DNA Sample 10μg Possible Father #1 DNA Sample

DOT Info: Non-regulated

IS3303 $101.97

Agarose Dye Marker

Agarose dye marker set can be used with a 2% agarose gel to get students accustomed to the electrophoresis process without using expensive DNA. You can run up to 8 gels.

Kit Includes: 1mL 1mL 1mL 1mL 1mL 1mL Bromophenol Blue Xylene Cyanol Orange G Crystal Violet Malachite Green Dye Mixture

DOT Info: Non-Regulated

Prepared Agarose

Simply melt the prepared agarose in a water bath or microwave. Pour into a gel casting tray, allow the molten agarose to solidify and run your gel.

IS5201 $12.20

0.8% Agarose 200mL - Resolves DNA fragments 0.6-35kb long

IS5202 $12.36

1.0% Agarose 200mL - Resolves DNA fragments 0.5-20kb long

IS5203 $14.25

2.0% Agarose 200mL - Resolves DNA fragments 0.1- 5kb long

WARNING: This product can expose you to chemicals including Crystal Violet, which is known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.

IS5007 $10.00

For Biotechnology equipment and Histology Reagents please visit our website!

DOT Info: Non-Regulated

Powdered Agarose

Prepare your own agarose gels at any concentration with the addition of TBE running buffer. Can be stored at room temperature for years.

IS5204 - Agarose 5 gram bottle (Low EEO) $15.50

IS5205 - Agarose 25 gram bottle (Low EEO) $53.95

Agarose Gel Reagents

IS5206 - TBE Buffer 5XConcentrate 500mL. 5X running buffer concentrate makes 2.5L of 1X working concentration

$12.25

IS5207 - Tris-EDTA (TE) Solution 10X concentrate 25mL. Use for diluting DNA samples

$4.95

IS5208 - Loading Dye 10x 5mL. Dye that is used for tracking DNA during agarose electrophoresis. $6.15

IS5209 - DNA20x Stain 60mL bottle. Stain and Destain in 40 Minutes. This highly sensitive stain will stain a gel in about 20 minutes and destain in 20minutes. Once the gels are stained they can be stored for months without fading. Stain up to 20 gels with 60mL. $8.95

IS5250 - Agarose Gel Electrophoresis Reagent Pack. All the necessary reagents to prepare and run agarose gels in one convenient package. Contains: TBE buffer, 5x concentrate 500mL; Agarose, powdered, 5g; DNA Stain 20x concentrate, 60mL; Loading Dye, 10x concentrate, 10mL $41.15

DOT Info: Non-Regulated