Knowing the Chromatography Workflow Consisting of many different and evolving techniques, chromatography is used across multiple branches of science to separate molecules. With a focus on preparation and/or analysis of these molecules, there are two main phases of chromatography used for separating components: the mobile phase and the stationary phase. For glassware, labware and life science benchtop equipment suggestions by chromatography phase, please see the following pages.
Preparation
When preparing the column, careful thought must be given to provide best sample separation. Consider sample polarity, size, and affinity. Preparation is dictated by what type of chromatography is used – analytical or preparative.
Running the Column
Columns must be set up properly for samples to run effectively. Factors like loading the column or making sure it is vertically straight, could make a vast difference in sample collection.
Fraction Collection
Fraction collection varies, depending on how well you can visualize your sample on the column. Best practice dictates to collect many small fractions which can be pooled together once their purity is confirmed.
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Analysis
Thin layer chromatography is a common technique to confirm which fraction contains the desired eluent. Additional techniques like UV/Vis spectrophotometry can be used separately or in concert.
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Concentration
Often, the pure sample must be at a higher concentration than what the pooled fractions permit. Tools such as evaporators are available.
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Clean Up
Properly cleaned glassware and plasticware allows for confidence in future analyses using the same methodology.
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Glassware, Labware, & Benchtop Equipment for
The Chromatography Workf low SP chromatography labware, glassware and life science benchtop equipment are developed with the scientist in mind, helping to find a more efficient solution for individual workflows. The comprehensive and cross-discipline tools available are outlined on the following pages.
Preparation Mobile phase must be mixed and stored properly. Our products offer novel solutions to keep your mobile phase mixed and contained.
Running the Column
Fraction Collection
When setting up your SP Wilmad-LabGlass chromatography columns, tools are needed to ensure your mobile phase is added gently to the column for the tightest sample band.
SP Bel-Art labware carries various rack sizes for any scale of collection – from microcentrifuge tube to beaker.
Analysis
Concentration
Clean Up
SP Wilmad-LabGlass has chambers and reagent atomizers that you need to visualize your eluent.
Using the most advanced proven technology in evaporation science, SP Genevac evaporators are designed for solvent removal for the concentration of samples.
Test tubes can be disposed of after use, but can also easily be washed using SP Bel-Art test tube baskets or SP Hotpack glassware washers.
Running the Column
Preparation
Fraction Collection
HPLC Reservoir Secondary Containers
Chromatography Column Holder
Polypropylene Graduated Griffin Low Form Beakers
Stainless Steel Micro Spoon and Spatula Weighing Set
Lattice Support Stand
Polypropylene Conical Tube Holder; For 15ml and 50ml Tubes
Pickup Adapter w/Valve
Economy Support Stand
Microcentrifuge Tube Ice Rack/Tray
MP Cap 3-Hole Economy
Heavy Duty Support Stand
Polypropylene Wire Rack I.D. Tags
Red GL45 Cap w/PTFE Liner
Polypropylene Compact Support Stand
Write-On Label Tape Multi-Pack
MP Cap 3-Hole GL45
Stainless Steel Bosshead
Poxygrid Test Tube Racks
Glass Frit
Spring Action 9 in. Burette Clamp Clear View Polycarbonate Fume Hoods
Indexed Test Tube Racks
Fabricated Polypropylene Fume Hood
Reversible PCR and Microcentrifuge Tube Racks
Flash Chromatography Reservoir
Microcentrifuge Tube Racks
Flow Controller
Low Form Beaker
MP Cap Filter/Degassing
Polypropylene Drum and Carboy Funnels Polypropylene Micro Funnels Polypropylene Standard Stem Funnels Poxygrid Pasteur Pipette Can Rack Roto-Flo Polystyrene Flow Indicators Clear Polycarbonate Flow Indicator Vikem Vinyl Pipette Filler Flash Chromatography Column w/PTFE Plug Straight Chromatography Columns w/PTFE Plug Straight Chromatography Columns w/PTFE Plug & Fritted Disc Straight Chromatography Columns w/PTFE Plug & Fritted Disc
No-Wire Test Tube Grip Racks
Analysis
Concentration
Clean Up
Complete TLC Developing Tanks
EZ-2 4.0 Benchtop Evaporator
Polyethylene Safety Waste 20 Liter (5 Gallon) Jug
TLC Developing Tanks w/Screw Cap
HT Series 3i High Throughput Evaporator
GHS Labeled Right-to-Know Safety Vented Wash Bottles
TLC Sprayers
Round Bottom Flasks 1N
Polypropylene Wash Bottle Carrier
Clear Mini-Vials w/Screw Cap
Polyethylene Two Bottle Carrier
Standard RotoVap Evaporator Advanced RotoVap Evaporator
Polypropylene Test Tube Baskets & Lids Vakuwash Cuvette Washer Polypropylene Spill Containment Tray Glassware Washers
To contact our dedicated SP brand specialists, see below:
© 2022 Bel-Art Products, a part of SP. All rights reserved. SP Bel-Art, SP Hotpack, SP Genevac and SP Wilmad-LabGlass are brands of SP (scientificproducts.com), provider of biopharma processing equipment, life sciences equipment, scientific glassware and laboratory supplies used in the pharmaceutical, biotechnology, academic, food and beverage and industrial markets. All trademarks referenced in this document are registered or common law trademarks of SP Industries, Inc. or its affiliates in the U.S. and/or other countries.
Contact SP Bel-Art:
Contact SP Hotpack:
Contact SP Genevac:
Contact SP Wilmad-LabGlass:
Bel-Art Products, a part of SP 661 Route 23 South Wayne, New Jersey 07470 USA U.S.: +1-800-423-5278 Intl: +1-973-694-0500 cservice.belart@spindustries.com
SP Corporate Office 935 Mearns Road Warminster, PA 18974 USA U.S.: +1-800-523-2327 Intl: +1-215-672-7800 sales@spscientific.com
Genevac Ltd. Farthing Road, Ipswich Suffolk, IP1 5AP U.K.: +44 (0)1473 240000 Intl: +845-255-5000 sales@genevacusa.com
SP Wilmad-LabGlass, an SP brand 1172 NW Boulevard, Vineland, NJ 08360 USA U.S.: +1-800-220-5171 Intl: +1-856-691-3200 cs@wilmad-labglass.com
www.sp-belart.com
www.sp-hotpack.com
www.sp-genevac.com
www.sp-wilmadlabglass.com SP-05078-RevB