Other Popular “Apps”
Other Popular “Apps” In addition to traditional HPLC, over the past several years, there have been advances in technology that have expanded the world of chromatography in several directions. Two of these have become the most prominent — microscale chromatography and UHPLC, or Ultra-High Performance Liquid Chromatography.
Big things come in little packages One of the most popular advances in chromatographic technology has been the development of microscale and even nanoscale chromatographic methods. Where HPLC uses things like 1/16” (1.6 mm) OD tubing, flow rates of 1 mL/min, and sample sizes of 20 µL, this smaller-scale chromatography is known for much different “standards”: 360 µm OD tubing, flow rates in the low microliters per minute or less, and sample sizes in the nanoliter range. Somewhat intuitively, this smaller scale requires an increased attention
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to the quality of the connections that are made, as even small dead volume chambers can have a tremendous impact on your results when operating under these conditions. Working to ensure your tubing is cut and prepared well (see page 44 for a discussion on how to properly prepare tubing) and making sure that you are using the best fittings for the application (see page 39 for a discussion on “pitfalls” to avoid when connecting capillary tubing and tips for making good connections with capillary tubing) will pay back huge dividends in the form of high quality chromatographic results.
FITTINGS 101
