Vitronectin - Animal Free Cell Culture Matrix Kit Now is the time to shift from Matrigel™ to Animal Free Vitronectin cell culture surface-coating reagent
Qualified for hESC/iPSC
The extra cellular matrix (ECM) plays and important role in the stem cell niche. In-vitro stem cell cultures have utilized feeder cells and an undefined, animal derived ECM. Current stem cell research needs calls for defined culture conditions, including the use of defined recombinant ECM. Kit Components (Cat# AF-VMB-220): 1. Animal-Free Human Vitronectin Matrix 2. Animal-Free Vitronectin Matrix Buffer (PBS + Kolliphor P 188) for Vitronectin Matrix reconstitution and cell plate/dish dilutions. Kolliphor P 188 is a solubilizer that acts as a dispersing and wetting agent for optimal coating of cell culture vessels with vitronectin. The kit allows the coating of 100 wells of 6-wells plates with vitronectin.
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Defined
Animal Free
Pricing
Using a defined recombinant protein.
Using an Animal-Free protein that avoids hazards connected with reagents from animal origin.
$60 for coating 100 wells of 6-wells plates.
Reference Medium on Matrigel™ 200x magnification
PeproGrow-hESC Medium on Matrigel™ 200x magnification
Reference Medium on Vitronectin 200x magnification
PeproGrow-hESC Medium on Vitronectin 200x magnification
The reference medium produces well-formed tightly packed colonies, on both Matrigel and Vitronectin. Cells grown in the PeproGrow-hESC medium on both matrices retain the same morphological characteristics of the cells grown in the reference medium.
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AF-PeproGrow-hESC
DAPI: Nanog: Oct4: TRA-1-60:
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Reference Medium
V I T R O N E C T I N AF-PeproGrow-hESC
Nuclear counterstain Pluripotency marker, transcription factor expressed in undifferentiated cells Pluripotency marker, transcription factor expressed in undifferentiated cells Pluripotency marker, expressed on the surface undifferentiated cells
HFF IPSCS CULTURED IN LOW PROTEIN AF-PEPROGROW HESC MEDIUM ON AF-VITRONECTIN
DAPI: Oct4: SSEA1: SSEA4:
Reference Medium
H1 HESCS CULTURED IN LOW PROTEIN AF-PEPROGROW HESC MEDIUM ON AF-VITRONECTIN
Nuclear counterstain Pluripotency marker, transcription factor expressed in undifferentiated cells Differentiation marker, expressed on the surface differentiated cells Pluripotency marker, expressed on the surface undifferentiated cells
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