Efficacy of triclabendazole and prevalence of Fasciola hepatica in dairy cattle raised 3200 meters above the sea level in the provinces of Cajamarca, Hualgayoc and Celendín, Cajamarca-Perú. 1 E. ;
Jierson Mendoza A2173
Pedro Ortiz O.²
1Fondo de Crédito para el Desarrollo Agroforestal-FONCREAGRO,Cajamarca, Perú. 2 Facultad de Ciencias Veterinarias, Universidad Nacional de Cajamarca, Perú. Email: Jierson_mendoza@yahoo.com Background
Bovine fasciolosis caused by Fasciola hepatica is one of the greatest health problems facing dairy farmers in the provinces of Cajamarca, Hualgayoc and Celendín in the northern highlands of Peru, 3200 meters above the sea level. Most of them are small producers with six animals on average. The operation performed by these producers is characterized by using fewer fasciolicides treatments per year and the lack of technical assistance compared with producers in the valley, Drug control is done mostly using triclabendazole (TCBZ). The objectives of the present research were: To evaluate the efficacy of TCBZ against F. hepatica in dairy cattle raised 3200 meters above the sea level in the Provinces of Cajamarca, Celendín and Hualgayoc and to determin the prevalence of F. hepatica in these animals.
Materials and methods Study area The study was conducted in 23 villages of the Provinces of Cajamarca, Celendín y Hualgayoc, located between 3370 to 3964 meters above the sea level.
Fig 2. Calculation of the body weight
Fig 1. Location of the study area
Methodology The efficacy of TCBZ was carried out by faecal egg count reduction test (FECT). Parasitological stool examination was perfomed by the sedimentation technique. To evalute the efficacy of TCBZ 120 cattle naturally infected with F. hepatica were treated with TCBZ at 12% of at a dose of 12mg/ Kg of body weight. Samples were collected 3 days before treatment and 28 days after treatment. The percentage of efficacy was calculated using the following formula:
Fig 3. Dosing the animals
The prevalence was determinated by a study conducted between september 2012 to March 2014. A total 0f 1231 stool samples from the animals in the research were analized by the sedimentation technique. The percentage of prevalence was calculated using the following formula :
Results Table 1. Efficacy of TCBZ against Fasciola hepatica in dairy cattle raised 3200 meters above the sea level in the Provinces of Cajamarca, Celendín y Hualgayoc.
Provinces
Cajamarca
N° Farmers
8
N° Positive animals
38
FEC/gr Pre treatment X ± DS
ƩFEC
3.97 ± 2.77
151
Range
FEC/gr Post treatment (28 days) X ± DS
1 a 17 1.13 ± 1.26
Table 2. Prevalence of Fasciola hepatica in dairy cattle raised 3200 meters above the sea level in the Provinces of Cajamarca, Celendín y Hualgayoc.
% Range Efficacy
ƩFEC 43
0a6
Provinces
Total positive animals
Total sampled Prevalence (%) ± IC animals
Cajamarca
438
645
67.91 ± 3.60
Celendín
261
394
66.24 ± 4.67
Hualgayoc
128
192
66.67 ± 6.67
General Prevalence
827
1231
67.18 ± 2.62
71.52
Celendín
12
59
2.18 ± 1.41
129
1 a 8 0.37 ± 0.92
22
0a4
82.95
Hualgayoc
9
23
2.06 ± 1.75
60
1 a 8 0.34 ± 0.71
8
0a3
86.67
General efficacy
29
120
2.83 ± 2.53
340
1 a 17 0.61 ± 1.15
73
0a6
78.53
Conclusions The clinical efficacy of Triclabendazole in dairy cattle raised 3,200 meters above the sea level is 78.53% The prevalence of F. hepatica in this area is 67.18 %.
References
Brockwell, YM, Elliot, TP, Anderson, GR, Stanton, R, Spithill, TW & Sangster, NC, 2014. ´Confirmation of Fasciola hepatica resistant to triclabendazole in naturally infected Australian beef and dairy cattle´, International Journal for Parasitology: Drugs and Drugs Resistance, 4, pp, 48-54. Ortiz, P, Scarcella, S, Cerna, C, Rosales, C, Cabrera, M, Guzmán, M, Lamenza, P, & Solana, H 2013. `Resiatance of Fasciola hepatica against Triclabendazole in cattle in Cajamarca (Perú): A clinical trial and an in vivo efficacy test in sheep.´, Veterinary Parasitology, 195, pp, 118-121. Olaechea, F, Lovera, V, Larroza, M, Raffo, F & Cabrera, R 2011. `Resistance of Fasciola hepatica against triclabendazole in cattle in Patagonia (Argentina)´, Veterinary Parasitology, 178, pp, 364-366.