SCIENCE PHOTOGRAPHY CAPTIONS CELEBRATING 50 YEARS OF NEUROSCIENCE PROGRESS
A History of the Society for Neuroscience
Cover
Pages 04/05
Page 17 (middle image)
This image shows a retinal ganglion cell that was biolistically labeled in an adult mouse. Cytosolic expression of fluorescent protein tdTomato (blue) reveals cellular morphology, while coexpression of YFP-tagged PSD95 (yellow) labels excitatory postsynaptic sites within the same neuron. The left portion of the image represents the rendered volume of fluorescent signals expressed by the cell, gradually blended with its digitized representation of the dendritic arbor’s skeleton (blue lines) and synaptic loci (pink spheres).
This image shows mature cochlear heminodes beneath hair cells and nodes of Ranvier within osseous spiral lamina in adult mouse auditory nerve. The nodes and their flanking paranodes were immunolabeled for neuronal cell adhesion molecule (NrCAM, green) and contactin 1 (Cntn1, red), respectively. Myelin of the auditory nerve (following the heminodes) was detected by immunolabeling for myelin basic protein (MBP, blue; nuclei were counterstained with DAPI also in blue). The integrity of myelin and nodal structures in the cochlea is needed for fast transfer of sound information from the hair cells to the brain.
These vasoactive intestinal peptide (VIP)-expressing interneurons in mouse somatosensory cortex were targeted with a modified rabies virus system to label their brain-wide monosynaptic inputs. Many VIP-expressing interneurons exhibit a striking bipolar morphology, with primary neurites that run perpendicular to the cortical surface.
Courtesy with permission: Yvonne Ou, Rebecca E. Jo, Erik M. Ullian, Rachel O.L. Wong and Luca Della Santina, 2016, Journal of Neuroscience , 36 (35) 9240–9252
Contents This image shows cone photoreceptors directly contacting microglia within the outer plexiform layer of the human retina. The tissue was immunolabeled with antibodies against calbindin (green) and peanut agglutinin (blue), and microglia were labeled with monocyte marker ionized calcium-binding adapter molecule 1 (red). Microglia, photoreceptor interaction plays an important role in postnatal photoreceptor maturation, with loss of fractalkine-Cx3cr1 signaling leading to an altered distribution of cilium proteins, failure of outer segment elongation, and cone photoreceptor loss. Courtesy with permission: Andrew I. Jobling, Michelle Waugh, Kirstan A. Vessey, Joanna A. Phipps, Lidia Trogrlic, Una Greferath, Samuel A. Mills, Zhi L. Tan, Michelle M. Ward and Erica L. Fletcher, 2018, Journal of Neuroscience 38 (20) 4708–-4723
Pages viii/01 This image shows specialized functional domains along myelinated axons in mouse sciatic nerve. Immunostaining for neurofascin (blue) is present in the axolemma at nodes of Ranvier (strong staining of NF186) and in paranodal glia (weak staining of NF155). Immunolabeling of contactin-associated protein (green) reveals paranodes, and immuolabeling of voltage-gated K+ channels (red) shows juxtaparanodes. Courtesy with permission: Keiichiro Susuki, Daniel R. Zollinger, Kae-Jiun Chang, Chuansheng Zhang, Claire Yu-Mei Huang, Chang-Ru Tsai, Mauricio R. Galiano, Yanhong Liu, Savannah D. Benusa, Leonid M. Yermakov, Ryan B. Griggs, Jeffrey L. Dupree and Matthew N. Rasband, 2018, Journal of Neuroscience , 38 (27) 6063–6075
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Courtesy with permission: Clarisse H. Panganiban, Jeremy L. Barth, Lama Darbelli, Yazhi Xing, Jianning Zhang, Hui Li, Kenyaria V. Noble, Ting Liu, LaShardai N. Brown, Bradley A. Schulte, Stéphane Richard and Hainan Lang, 2018, Journal of Neuroscience , 38 (10) 2551–2568
Page 09 This image shows a cross section of a day 28 human forebrain organoid, showing FOXG1-expressing neural precursors (Red), surrounding a ventricle-like structure outlined by N-Cadherin staining (Green). DAPI staining is blue. Courtesy with permission: Ai Tian, Julien Muffat and Yun Li, 2020, Journal of Neuroscience , 40 (6) 1186–1193
Page 14/15 This live Airyscan image shows a zebrafish neuromast with hair cells expressing the red calcium indicator RGECO1 (magenta) and the innervating afferent process expressing GFP (neurod: EGFP, green). Courtesy with permission: Lavinia Sheets, Xinyi J. He, Jennifer Olt, Mary Schreck, Ronald S. Petralia, Ya-Xian Wang, Qiuxiang Zhang, Alisha Beirl, Teresa Nicolson, Walter Marcotti, Josef G. Trapani and Katie S. Kindt, 2017, Journal of Neuroscience , 37 (26) 6299-6313
Page 17 (top image) This image shows mitochondria (magenta) in the processes of primary oligodendrocytes expressing myelin basic protein (green). The oligodendrocytes were purified via magnetic activated cell separation and cultured for 10 days in vitro. Courtesy with permission: Kelly A. Chamberlain, Kristen S. Chapey, Sonia E. Nanescu and Jeffrey K. Huang, 2017, Journal of Neuroscience , 37 (6) 1479–1492
Courtesy with permission: Nicholas R. Wall, Mauricio De La Parra, Jordan M. Sorokin, Hiroki Taniguchi, Z. Josh Huang and Edward M. Callaway, 2016, Journal of Neuroscience 36 (14) 4000–4009
Page 17 (bottom image) Abundant α-synuclein inclusions (green) localize throughout axons (magenta). Courtesy with permission: Laura A. Volpicelli-Daley, Hisham Abdelmotilib, Zhiyong Liu, Lindsay Stoyka, João Paulo Lima Daher, Austen J. Milnerwood, Vivek K. Unni, Warren D. Hirst, Zhenyu Yue, Hien T. Zhao, Kyle Fraser, Richard E. Kennedy and Andrew B. West, 2016, Journal of Neuroscience , 36 (28) 7415–7427
Page 19 This image acquired with super resolution STED microscopy shows a fixed cortical axonal growth cone stained for F-actin (magenta) in the growth cone periphery and microtubules (cyan) in the center. The entry of single microtubules into filopodia and extension along actin filament bundles is regulated by the microtubule associated protein tau. Courtesy with permission: Sayantanee Biswas and Katherine Kalil, 2018, Journal of Neuroscience , 38 (2) 291–307
Pages 20/21 This image shows a cortical neuron from an embryonic day 14 wild-type mouse grown in culture for 7 days. The cell was immunostained with antibodies against phosphorylated Src/Fyn (pY527, green), Src (red), and the microtubule-associated protein MAP2 (blue) antibodies. The pY527 signal is localized in dendritic growth cones. Sema3A stimulation decreases the pY527 signal in wild-type neurons, but not in those lacking the protein tyrosine phosphatase Ptpδ. Courtesy with permission: Fumio Nakamura, Takako Okada, Maria Shishikura, Noriko Uetani, Masahiko Taniguchi, Takeshi Yagi, Yoichiro Iwakura, Toshio Ohshima, Yoshio Goshima and Stephen M. Strittmatter, 2017, Journal of Neuroscience , 37 (30) 7125–7139
