D-CELL BEROCHURE

Tissue Regeneration Activator

What is

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D+Cell 350 TRA is the dermal care product for the professional use only with PDRN which has treated for wound and ulcer as a medicine. As well, D+Cell 350 TRA is optimized for various skin care and treatment processes, and furthermore the efficacy and safety were approved through clinical studies by dermatological experts.

I II W

: Mesotherapy, Trandermal use, Scar, Recovering dermis & Scalp : Fraxel Laser, IPL, MTS, Iontophoresis, Electroporation : Local Whitening

Cream

: After peeling, Homecare after treatment

Outstanding characteristics of D+CELL 350 TRA is the skin revitalization treatment that other products and treatments up to now cannot do without. That is because D+CELL 350 TRA with cell generation and cell growthpromotion ingredients offers the perfect way to activate excellently fast and comparable skin tissue regeneration. Moreover, as these ingredients were already approved and have been used for the medicals, D+CELL 350 TRA can be safe, and also specifically can be suited for use in more sensitive skin areas.

1. Regernate dermal tissues - Increase the number of fibroblast in dermis - Promote to secrete the substances as like fibroblast, of which skin composed.

- Generate new capillaries in skin and improve the microvascular circulation - Provide scarless wound healing without hypertrophy by feedback system.

- Decrease swelling by anti-inflammatory and anti-edemic effects 2. Maintain the tissue regeneration activation and conditions for longer than any other does 3. Prevent cells from skin aging and recover damaged cells by promoting DNA biosynthesis

Mechanism of action (Tissue regeneration activator) A2 Pur ine receptor agonist Salvage pathway stimulation

Cell growth activation by promoting DNA synthesis PDRN, as tissue regeneration activator (TRA), activates the proliferation of fibroblasts in demis, and this effect is dose-dependent in a certain dose range.

Fibroblast proliferation

Control : Dulbecco’s modified Eagle’s medium(DMEM) with 1% fetal calf serum PDRN

: Dulbecco’s modified Eagle’s medium(DMEM) with 1% fetal calf serum + 10ug/ml PDRN

Dose dependent Fibroblast proliferation

Ref. S. Thellung et al, Polydeoxyribonuleotides enhance the proliferation of human skin fibroblasts, Life science V ol 64. No 18 1661-1674, 1999

Stimulation on various growth factors PDRN, as a tissue regeneration activator (TRA), interacting with A2 purinergic receptor stimulates the secretion of various growth factors as below.

VEGF Vascular endothelial growth factor (VEGF) is known a kind of growth factor as an important signaling protein to stimulate a new blood vessel formation and growth. VEGF is a part of restoration system for oxygen supply when blood circulation is inadequate.

bFGF During wound healing, various enzymes activate basic fibroblast growth factor (bFGF), thus promoting the growth of fibroblasts, mediating the formation of dermal tissues and stimulating capillary generation.

IGF-1 Insulin-like growth factor (IGF-1) through AKT pathway plays an important role in promoting cell growth and proliferation of muscles, cartilage, nerve and skin, and can delay program cell death.

IL-8 IL-8(Interleukin-8) which is a chemokine signals other immune cells to come in to the site of inflammation, and mediates these immune cells can reduce the inflammatory response.

Angiopoietin Angiopoietin is a protein growth factor to promote the formation of blood vessels from pre-existing blood vessels unlike VEGF.

I

- Tissue regeneration solution

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D+CELL 350 TRA I vitalizes the regeneration and growth of tissues in dermis. And these actions induce to fill up the depressed tissues of the dermis and allow to re-fomulate the skin tissues into healthy conditions.

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D+CELL 350 TRA I activates the secretion and proliferation of fibroblast. The increase of fibroblast produces various components of dermis such as collagen, elastin, reticular fiber and GAGs (Glycosaminoglycans), and these components enlarge dermis as a result. Furthermore, its advantageous effects can be maximized when it is treated with proper processes using in cosmetic and plastic operation.

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Also, D+CELL 350 TRA I improves skin elasticity in scalp, and specilly promotes the formation of a blood vessel as well as the growth of hair bulb.

II ■

- Esthetic regeneration solution

A variety of factors such as aging, UV and exposure to many chemical materials occur the reduction of fibroblasts and their activities. This reduction results in the decrease of components for skin and accordingly low skin elasticity. Different from skin cell vitalization by temporally stimulation or moisturizing, D+CELL 350 TRA II gives the proliferation of fibroblast to restore and boost skin elasticity and vitality. This treatment of TRA II can prolong the revitalizating effects due to the proliferation of fibroblast. That is the reason why D+CELL 350 TRA II is the ideal skin boosting treatment which nurtures the skin in a way that many other creams and moisturizers simply cannot do.

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TRA II can be used in conjunction with other aesthetic treatments such as CO2 fraxel laser, NdYag laser, IPL, MTS, FNS, chemo peel, etc. This skin conditioning approaches can provide more adaptable treatment for those special occasions to look the best.

W - Regeneration & Whitening solution ■

UV affects to the skin basal layer to make the keratin layer further thicker and causes more secretion of melanin pigment to make skin tone much darker. Also, as UV lessens the vitalization of skin, it enhances more skin photo-aging. However, as the proliferation of fibroblast caused by D+CELL 350 TRA W induces the regeneration and more vital activities of skin cells and tissues, it can help dead skin cells drop out and keratin layer become thinner, then to recover and improve skin tone.

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Typically, D+CELL 350 TRA W contains arbutin, which was identified for whitening effect from KFDA, and placental extract to inhibit the activity of tyrosinase as a core element for the biosynthesis of melanin pigments.

Cream ■

– Maintenance cream

D+CELL 350 TRA Cream is skin regeneration cream which includes PDRN, tissue regeneration activator. PDRN is reported to assist the production of the components of the extracellular matrix. This considerable property of PDRN makes it the best choice obviously for skin areas that take on a dehydrated and less vital appearance with over exposure to the UV as well as physical and chemical responses.

I

Tissue regeneration solution

II

Esthetic regeneration solution

W Regeneration & Whitening solution ■ STERILE 2.2ml × 5 Vials

Cream

Maintenance cream

■ 30g

References ① P. Sini et al., Effect of polydeoxyribonucleotides(PDRN) on human fibroblasts in primary culture: Cell Biochemistry and Function Vol.17 1999, 107 - 114 ② Thellung S et al., Polydeoxyribonucleotide(PDRN) enhances the proliferation of human skin fibroblasts: Involvement of A2 Purinergic receptor subtypes: Life Sciences Vol.64 No.18, 1999, 1661 - 1674 ③ S. Guizzardi et al., Polydeoxyribonucleotide(PDRN) promotes human osteoblast proliferation: A new proposal for bone tissue repair: Life Sciences Vol.73 2003, 1973 - 1983 ④ A. Bitto et al., Polydeoxyribonucleotide(PDRN) improves angiogenesis and wound healing in experimental thermal injury: Critical Care Vol.36, No.5 2008, 1594 - 1602 ⑤ M. Galeano et al., Polydeoxyribonucleotide(PDRN) stimulates angiogenesis and wound healing in the genetically diabetic mouse: Wound Repair and Regeneration Vol.16 2008, 208 - 217 ⑥ Rubegni P et al., Clinical evaluation of the trophic effect of polydeoxyribonucleotide(PDRN) in patients undergoing skin explants: Current Medical Research and Opinion Vol.17, No.2 2001, 128 - 131 ⑦ G. D. Aloe et al., Skin graft donor site and use of polydeoxyribonucleotide as a treatment for skin regeneration: A randomized, controlled, double-blind, clinical trial: WOUNDS Vol.16, No.8 2004, 1 - 6 ⑧ G. Magi et al., Clinical assessment of the effect of polydeoxyribonucleotide in trophic lesions of the lower limbs in chronic vasculopathic patients: Minerva Angiologica Vol.16, No.2 1991, 437 - 440 ⑨ M. C. Masnada et al., Sull’impiego di un estratto di placenta per via intradermica in lesion acneiche cicartriziali: La Medicina Estetica Vol.11, No.2 1987, 69 - 71 ⑩ M. Vigano., Utilizzo di un polodesossiribonucleotide(PDRN) nelle ustioni di grado intermedio: Giornale Italiano di Ricerche Cliniche e Terapeutiche Vol.11, No.5 1990, 90 - 93 ⑪ L. Valdatta et al., Evaluation of the efficacy of polydeoxyribonucleotides in the healing process of autologous skin graft donor sites: Current Medical Research and Opinion Vol.20, No.3 2004, 403 - 408 ⑫ 김요한 등, 전층피부 결손에서 PDRN의 창상치유 효과, 대한성형외과학회지, 제37권, 제3호,. 220 - 226, 2010

■ 판매원

㈜ 파마리서치

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