Enhancing Motor Function in Multiple Sclerosis

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Acaseseriesinindividualswithmultiple sclerosisusingdirectcurrentelectrical stimulationtoinhibitspasticityandimprove functionaloutcomes

Abstract

BackgroundandPurpose: Multiplesclerosis(MS)hasahighincidenceofdebilitatingspasticity.Central NervousSystem(CNS)intrafusalsettingshaveanimpactonspasticitylevel.Mechanoreceptorsofthe PeripheralNervousSystem(PNS)communicatemonosynapticallywiththecentralnervoussystem (CNS).ThiscaseseriesassessesfeasibilityofmultimodaltreatmentofindividualswithMSusinga directcurrentelectricalstimulation(DC)toinfluencemechanoreceptors.

CaseDescriptionandIntervention: SevenMSdiagnosedparticipantswithExpandedDisabilityStatus Scale(EDSS) = 6.0–8.0completed18visitsover6weeksofusingDCcombinedwithneuromuscular reeducation.Designincludedpre-,post-outcomemeasuresofEDSS,12-itemMSWalkingScale (MSWS-12),RangeofMotion(ROM),ManualMuscleTesting(MMT),ModifiedAshworthTest (MAT),Timed25-Footwalk(T25WT),TimedUpandGo(TUG)andtheMultipleSclerosisImpact Scale-29(MSIS-29).

Outcome: 125outofapossible126visitswerecompleted,demonstratingahighleveloftolerance. Individualresultsincludedtrendstowardsimprovementinspasticityandagonists.

Discussion: ThiscaseseriesdesignofsevenheterogenoussubjectswithMSisalowsamplesizeforstatisticalanalysisandshouldbeconsideredapilot.Thestudydemonstratesahighleveloffeasibilityand possiblecorrelationstoconsider.Furtherresearchiswarranted.

Keywords: multiplesclerosis,spasticity

Datereceived:30March2023;accepted:20June2023

Introduction

Approximately66%1–84%2,3 ofindividualswith multiplesclerosis(MS)experiencespasticity. 1 The pathophysiologyofspasticitybestunderstood, demonstratesanimbalancebetweeninhibitory dorsalreticulospinaltract(RST) fibersandexcitatory bulbopontinetegmentum.RSTneuronsreceivedirect somatic,vestibular,tectal,cerebellarandmotorexcitatoryinputandarescatteredintheventraland lateralspinalcordcolumnsintermingledwithpropriospinal fi bers. 2 TheperipheralGolgitendon organofmuscletendonandmusclespindleintrafusal fi bersubiquitousthroughoutthemusculoskeletal systemhavemonosynapticconnectiontothiscentral

nervoussystem(CNS)propriospinal fi bersandin fl uencethedegreeofspasticityandoveractivestretch re fl exes. 2,4

Whenmechanoreceptorsfunctionnormallywithina fullyintactneurologicalsystem,theyareprotective againststretchinmuscle,tendonand/orboneinjury causingreflexivecounterforcesuchasdroppinga weightthatistooheavy.2 InanindividualwithMS theCNScausesthethresholdforstretchreflexand muscletightnesstobesetlowerthannormal.2 This inhibitsnormalmovementandcausesabnormalities inambulation,posture,stiffnessandattimesjoint contractures.

MultipleSclerosisJournal Experimental,Translational andClinical

July-September2023,1–12

DOI:10.1177/ 20552173231186512

©TheAuthor(s),2023. Articlereuseguidelines: sagepub.com/journalspermissions

Correspondenceto: CourtneyLEllerbusch,PT, DPT,CenturaHealthat Home. courtneyellerbusch@ centura.org

CourtneyLEllerbusch, AmbulatoryDepartment, CenturaHealthat Home,Denver,Colorado, USA

KristinaMChapple, DepartmentofSurgery, UniversityofArizonaSchool ofMedicine,Phoenix, Arizona,USA

JulieBSeibert, MultipleSclerosis,

permissionprovidedtheoriginalworkisattributedasspecifiedontheSAGEandOpenAccesspage(https://us.sagepub.com/en-us/nam/open-access-at-sage).

OverseeingNeurologist, Littleton,Colorado,USA

Ifdirectcurrentelectricalstimulation(DC)isableto altertheproprioceptionoftheperipheralnervous system(PNS)withinputtomechanoreceptors,it maybepossibleto findmeasurablechangeinCNS drivenspasticityduringtreatmentandaftertreatment.5 IfindividualswithMScanexperienceanormalizedstretchresponse,itmaybepossibleto createCNSlevelchangeswithinthebulbopontine tegmentum.Thispilotstudycaseseriesisdesigned withmeasuresofspasticity,rangeofmotion,muscle strength,gaitspeedandbalancetoassessfeasibility. ThepurposeofthisPilotcaseseriesistoassessif treatmentofindividualswithprogressiveMSatthe PNSlevelthroughtargetingmechanoreceptorsusing theNeuBie(NeurologicalFitnessEquipmentand EducationLLC,AustinTXmanufacturedbyJohari ElectroTechCompanyinJodhpurIndia),aDCelectricalstimulationdevice,hasfeasibilitytoinhibit spasticityandimprovefunctionalmobilityinclinicallysignificantmeasures. 6

Patientinformation

CourtneyEllerbusch,PT,DPTdesignedandimplementedarepeatablestudydesignofhomehealth visitsforprogressiveMSinpartnershipwithDrJulie Seibert,MD.DrEllerbuschcompletedallassessments andinterventions.Acaseseriesof7participantswith progressiveMSwithmobilityimpairment(Expanded DisabilityStatusScale = 6.0–8.0)completed18visits over6weeksofhomehealth-deliveredphysical therapyfromJuly22,2021throughJune3,2022. ThedesignutilizedDCcombinedwithneuromuscular reeducationinmanualactivations,7 flexibilitytraining, neuromuscularreeducationandfunctionaltraining.Dr Seibertcompletedeachsubject’sofficevisitpriorto participation,consentsandEDSS;thenrepeated EDSSuponinterventiontimelinecompletion.(See Table1fordemographics).

Assessmentandinterventiontimeline

Thestudyassessmentandinterventiontimeline (Table2)detailstheassessmentsandinterventionparametersprovidedforallsevensubjectsoveraperiodof 6weeksofhomehealthphysicaltherapy.Theonly exceptionissubject7whodevelopedurinarytract infection(UTI)symptomsonthe18thvisitandwas notabletotolerate finalambulatoryassessments.

InitialPTvisitincludedmedicalhistory,goals, assessmentoffunctionalimpairmentsandoutcomemeasures.Subjects1–7wereaheterogenousgroupofnonambulatoryandambulatory.Afour-partintervention wasusedwitheachsubject.Part1***targetedneuropathywithaDCfootbathprotocol,whenneuropathy

wasreported.Part2**targetedareasofmeasuredspasticitythrough flexibilitycombinedwithDCtoarange thatelicitedastretchwithoutcreatingaspasmorspasticityresponse.Part3**combinedDCwithagonist strengtheningthroughtoleratedrangeswithoutidentified compensatorymovements.Subjectsweregivenworkto restratioof1:2upto1:4dependingonfatigueresponse withinvisitorreportedatthenextappointment.Part4 appliedDCwhiletraining subjectstothehighest degreeoffunctionaltrainingsafelytoleratedwhilereducingoreliminatingcompensatorypatterns.

Groupclinical findings

APhDlevelstatisticianprovidedquantitativeanalysis MATandMMT(seeTable3).Individualcases showedavarietyofquantitativechanges,withtrends towardsimprovementinplantar flexor(PF)spasticity perMAT(71.4%ofsubjectsimprovedinPFMAT). SubjectstrendedtowardimprovementinMMTofbilateralpsoas,bilateralquadratuslumborum,bilateral vastuslateralisandmedialis,bilateralgluteusmedius, bilateralhipadductorgroup,bilateraltibialisanterior (TA)andbilateralbicepsfemoris.Therearenotrends acrosssubjectsdemonstratingworseningmeasurements inspasticity,MMTorROM.Therearenocleartrends identifiedfromsubjectivemeasuresMSIS-29or MSWS-12acrosssubjects.Outofapossible126total visitsforsevensubjectsatotalof125visitswerecompleted,demonstratingahighleveloftolerance.One missedvisitoccurredduetopre-existingcomorbidities insubject7withUTIsymptoms.Allsubjectswereeducatedtothepotentialforneurologicalfatiguewith carefulassessmentofresponse.8 Subjects1and5 requiredinterventionmodificationindosingandintensityduetofatigueresponse,butabletotoleratemodifiedintensity.Subject7hadhypertensionattimesand UTIsymptomsrequiringmodifications.Noothersubjectsrequiredinterventionmodificationindosingor exerciseintensity.Therewerenoknownadverse responsestotheinterventionsprovided.

Individualclinical findings

Subject1(non-ambulatorypowerchairdependent) demonstratedimprovementsinrightsidehip flexor lengthimprovingfrominitialassessment(IA)of four fingersinpronefromASIStomatsurfaceand 0degreeshipinternalrotationassociatedwithright 0/5psoasMMTtoa finalassessment(FA)ofthree fingersinpronefromASIStomatsurface,5 degreeshipinternalrotationand1+/5rightpsoas MMT.Subject1alsodemonstratedimprovement from3/4hipextensionMATatinitialassessmentto finalassessmentof0/4hipextensionMATwith abilitytocontractpsoasmusclesforthe firsttime

Table1. Demographics.

Subjectmain impairmentsandgoalsMedicationListComorbidities

Supportat home

Assistivedevicefor mobilityandrelated impairments

Type ofMS

Age(age ofMS diagnosis)Sex

SN

depression,backpain, continuousbilateral lowerlegclonusin weightbearing; musclespasms

Ambien;Baclofen10mg (40mgdaily); clonazepam;Gilenya; Inhaler;Lipitor; oxycodone;paxil; valium;vitaminD3; wellbutrin

Impairments: hyposensationT10 levelanddistally, clonus,spasticityand spasms,backpainand weaknesslimiting transfers.Goals: Reducetone manisfestations, reducebackpainand improvestrengthfor transfers

Liveswith spousewho worksfull time availableto supportin morningand evening.

150(39)MSPMSCustomlightweight manualwheelchair. Unabletoambulate duetoweakness, andclonus.

acetaminophen; amlodipine;amoxicillin potassium;baclofen 10mg(40mgdaily); cholecalciferol; fl uticasone;furosemide; gabapentin;ibuprofen; lorazepam;rosuvastatin; stoolsoftner;sertraline; spironolactone;tylenol PM bilateralknee fl exion contracture; hypertension; hyperlipidemia;neck pain;backpain;knee pain;formersmoker; ataxia

Impairments:bilateral knee fl exion contractures, spasticity,weakness limitingtransfersand bedmobilityGoals: reducebilateralknee fl exioncontractures, improvestrengthfor bedmobilityand transfers

Liveswith daughter whois primary caregiver andworking fulltime fromhome

269(40)FPPMScustompowerchair. Unabletoambulate duetobilateralknee contracturesand weakness.

formersmoker, depression (untreated),anxiety (untreated)

Impairments:spasticity, severebilateral neuropathy; depression,bilateral legweaknessworseon leftsidewithfoot drop.Goals:Improve balance,reduced spasticity,reduce neuropathy,improve walkingqualityand distance baclofen10mg(30mg daily);cyclobenzaprin; dalfampridine; ergocalciferol;Ocrevus

Liveswith parentswho supportas needed.

330(23)MPPMSSinglePointCanewith ambulationdistance andqualitylimited byataxia, neuropathyand spasticity.

Subjectmain impairmentsandgoalsMedicationListComorbidities

Supportat home

Assistivedevicefor mobilityandrelated impairments

Type ofMS

Age(age ofMS diagnosis)Sex

Table1. Continued. SN

Myrbetriqseizures,tornLACL, shorttermmemory loss

Impairments:cognitive de fi cit,leftleg weaknessand spasticity,fatigue Goals:Improvemental clarity,improve energy,improveleft legstrengthand walkingdistanceas wellasquality

liveswith daughters takingturns between theirhomes; parttime paid caregiver support

464(27)FPPMSSinglePointCanewith ambulationdistance limitedbyLside footdropand spasticity.

overactivebladder; musclespasms

atorvastatin;baclofen 20mg(80mgtotal); B-complex;benadryl; magnesium; fi shoil; ibuprofen;juiceplus supplement;niacin; Ocrevus;omeprazole; ropinirole;saw palmento;tadala fi l; tamsulosin;tizanidine; valium;vitaminD

Impairments:bilateralleg weaknessand hypertonicitywith greaterweaknessleft legandpainfulspasms rightleg,limited walkingqualityand distanceGoals:restore anymobilityor strengthtolegs,reduce footdrop,reduce spasms

Liveswithwife andsome childrenat home; spouseis primary caregiver andworking fulltime

559(44)MSPMSThreeWheeledwalker indoormobilityand powerchairfor community distances. Limitationin bilaterallower extremityweakness, spasticityandspasm impacting ambulation.

2009RTibialSpiral Fracture;ataxic movements;severe hyperre fl exia bilaterally;sustained bilateralclonus (continued)

baclofen10mg(30mg daily);dalfampridine; vitaminD3;medical marijuana;Ocrevus

Impairments:Rleg weakness,severe hyperre fl exia, sustainedbilateral clonus,lowerlegand footneuropathy, spasticgaitwithRfoot drop.Goals:Improve walkingtoleranceand quality,improveR anklemovement, reduceneuropathy

Liveswithout caregiver support; familyin areasupport asneeded butdonot knowhehas MS

639(35)MPPMSBilateralcaneswith intermittentRAFO usewithweakness andspasticity limitingambulation.

Subjectmain impairmentsandgoalsMedicationListComorbidities

Supportat home

Assistivedevicefor mobilityandrelated impairments

Age(age ofMS diagnosis)Sex Type ofMS

anxiety/depression; frequentUTIs; hypertension; dizziness;dysarthria; urinaryfrequency/ urgency;diploplia; ataxicrapid alternating movements

atoravastatin;carvedilol; chlorthalidone; hydralazine;lexapro; losartan

Impairments:Rleg spasticity,weakness andfootdrop;fatigue, limitedwalking distanceanddizziness Goals:ImproveRleg strength,improve walkingqualityand distance

Liveswith spousewho isprimary caregiver

775(53)FSPMSFourWheeledwalker withRAFOdueto footdropand spasticityforall walkinginhome andmanual wheelchairfor community distances.

sinceinitialassessmentat1+/5MMT.TheseimprovementscombinedwithabilitytoplaceelectricalstimulationonbilateralPFtopreventclonusinweightbearing allowedthissubjecttostandwithoutclonus,requiring maximumassistancefromtheinvestigator,while holdingthesink.Priortostudyparticipation,subject 1wasunabletostandduetoweakness,clonusand spasticity.Subject1reportsdisappointmentinhis results,expressinghopetohaveimprovedhisstanding andcontrolofspasmsmoresubstantially.

Subject2(non-ambulatorypowerchairdependent withbilateralknee flexioncontractures)demonstrated reductioninbilateralPFspasticityfrom3/4MAT bilaterallyatIAreducedto1+/4atFAandabletotoleraterollingfromsupinetoprone.Pronepositioning wasnottoleratedpriortostudyparticipationdueto weaknessandbilateralknee flexioncontractures puttinghip flexorsintoashortenedposition.Subject 2improvedknee flexioncontracturesfrombilateral IA0-70-130improvedtoFA0-20-130passive rangeofmotion.Duetoimprovedkneerangeof motionandeliminationofclonusinweight-bearing duringapplicationofelectricalstimulationtobilateral gastrocnemiusandsoleusregion,Subject2wasable toinitiatestandingtrainingforthe firsttimein10 years(perself-reportofthesubject)atsinkwithbilateralupperextremitysupportandmaximumassistance fromprimaryinvestigator.Subject2reportsfeeling looserinherlegsandstrongerfortransfers.

Subject3(bilateralspasticgaitpatternwithunilateral cane)demonstratedmixedresultsinspasticity, strength,ambulationtestsandsubjectivereporting onMSWS-12andMSIS-29withadiagnosisof untreateddepression.Thissubjectdemonstrated ninthvisitreassessment(RA)ofspasticityasworse insomecategoriesandimprovedinothersbutsignificantimprovementinmusclelengthandstrengthin mostcategorieswhichisassociatedwithimprovementinT25WTfrom15.86sandTUG15.38sat IAtoT25WT14.52sandTUG14.5satRA. However,atFA,hedemonstratesincreasedPFspasticityandstrengthastrendingworseassociatedwith slowerT25WTtimeof17.8sandTUGtimeof 16.06s.AtFA,Subject3experiencedanincreasein bilateralpedaledema,spasticityinlowerlegsand feetseeninincreasedPFspasticityanddecreased gaittestingspeedbutwasabletogenerallymaintain progressinspasticityandstrengthofhipsandknees atFA.Subject3reportsfrustrationwithhisdiagnosis anddisappointmentthattheinterventiondidnot improvehisneuropathyspasticityandwalking beyonda24-hperiodofimprovement. Table1. Continued.

Table2. Studyassessmentandinterventiontimeline.

JS: study wrapup visit

CE:PTdischarge fi nal visit18

CE: PTvisits10 –17

CE:PTre-assessment visit9

3.EDSS

6.ObjectiveOutcome measures: MSWS-12;ROM; MMT;MAT;T25WT; TUG; 7.SubjectiveOutcome measures:MSIS-29; MSWS12 (afterdatacollection performedtreatmentas detailedbelow)

9.duplicateofstep9 (manualactivations) 10.**duplicateofstep 10(DCcombined with fl exibility,ROM andfunctional training) 11.duplicateofstep11 (DCwithfootbath)

6.ObjectiveOutcome measures: MSWS-12;ROM; MMT;MAT;T25WT; TUG

9.duplicateofstep9 (manualactivations)

JS:InitialvisitCE:InitialPTvisit1CE:PTvisit2CE:PTvisits3 –8

9.duplicateofstep9 (manualactivations)

10.**duplicateofstep10 (DCcombinedwith fl exibility,ROMand functionaltraining) 11.***duplicateofstep 11(DCwithfootbath)

8.*NeuBieelectrical stimulationre-mapping (seeappendix) 10.**duplicateofstep10 (DCcombinedwith fl exibility,ROMand functionaltraining)

11.***duplicateofstep 11(DCwithfootbath)

9.duplicateofstep9 (manualactivations)

8.*NeuBieelectricalstimulation mapping(seeappendix)

4.HomeHealth admission

10.**duplicateofstep 10(DCcombined with fl exibility,ROM andfunctional training) 11.duplicateofstep11 (DCwithfootbath)

9.Manualactivationstoimpaired musclesandareasof hypertonicity

10.**Electrodeplacements × 8 based onmappingcombinedwith fl exibilityopposingspasticity, agonistROMcorrecting compensatorypatternsrepeated forqualityastolerated; functionaltrainingbasedon subject ’ sability(bedmobility, transfers,ambulation)

11.***Incasesofneuropathy providedelectricalstimulation footbathwithmanualactivations andagonistROMtraining × 10 –15min

5.PTdetailedstudy explanation 6.Objective Outcome measures: MSWS-12; ROM;MMT; MAT;T25WT; TUG; 7.Subjective Outcome measures: MSIS-29; MSWS12

1.NeuBiepilot study explanation

2.NeuBiepilot study consent form 3.EDSS

Legend:JS = DrJulieSeibert;CE = DrCourtneyEllerbusch.

*Seeappendix1forNeuBiemappingexplanation. **Seeappendix2fordetailsonDCparametersanddosing. ***Seeappendix3fordetailsonDCfootbathprotocol.

Table3. QuantitativechangesinMATandMMT.

MATRhip flexionchange0520.0%71.4%28.6% MATLhip flexionchange0610.0%85.7%14.3% MATRhipextensionchange12414.3%28.6%57.1% MATLhipextensionchange24128.6%57.1%14.3% MATRknee flexionchange14214.3%57.1%28.6% MATLknee flexionchange0340.0%42.9%57.1% MATRkneeextensionchange15114.3%71.4%14.3% MATLkneeextensionchange0340.0%42.9%57.1% MATRankledorsiflexionchange16014.3%85.7%0.0% MATLankledorsiflexionchange0700.0%100.0%0.0% MATRAnkleplantarflexionchange11514.3%14.3%71.4% MATLankleplantarflexionchange20528.6%0.0%71.4% MMTRpsoasChange0340.0%42.9%57.1% MMTLpsoasChange0250.0%28.6%71.4% MMTRectusabdominuschange0330.0%50.0%50.0% MMTRquadratuslumborumchange0250.0%28.6%71.4% MMTLquadratuslumborumchange0340.0%42.9%57.1% MMTRRectusfemorischange12414.3%28.6%57.1% MMTLrectusfemorischange13314.3%42.9%42.9% MMTRvastuslateralis&medialischange11514.3%14.3%71.4% MMTRvastuslateralis&medialischange0070.0%0.0%100.0% MMTRgluteusmediuschange0070.0%0.0%100.0% MMTLgluteusmediuschange0340.0%42.9%57.1% MMTRhipadductorchange11514.3%14.3%71.4% MMTLhipadductorchange0250.0%28.6%71.4% MMTR fibulariichange32242.9%28.6%28.6% MMTL fibulariichange23228.6%42.9%28.6% MMTRtibialisanteriorchange0250.0%28.6%71.4% MMTLtibialisanteriorchange11514.3%14.3%71.4% MMTRtibialisposteriorchange32242.9%28.6%28.6% MMTLtibialisposteriorchange23228.6%42.9%28.6% MMTRtoeextensionchange12414.3%28.6%57.1% MMTLtoeextensionchange23228.6%42.9%28.6% MMTRtoe flexionchange22328.6%28.6%42.9% MMTLtoe flexionchange14214.3%57.1%28.6% MMTlumbarextensionbilateralchange10420.0%0.0%80.0% MMTRgluteusmaximuschange0340.0%42.9%57.1% MMTLgluteusmaximuschange22328.6%28.6%42.9% MMTRbicepsfemorischange0240.0%33.3%66.7% MMTLbicepsfemorischange0150.0%16.7%83.3% MMTRsemitendinosissemimembranosischange0250.0%28.6%71.4% MMTLsemitendinosissemimembranosischange14214.3%57.1%28.6%

Legend:MAT = ModifiedAshworthTest;MMT = ManualMuscleTest.

Subject4(spasticleftlowerextremityimpactinggait withunilateralcane)demonstratedimprovementin leftlowerextremityMAT,MMT,T25WTandTUG testsfromIAtoFA.Subject4improvedTUGIAof

19.34sandT25WT17.64stoFATUG15.6sand T25WT16.36s.Shealsodemonstratedimprovement inanklespasticityfrom3/4MATPFtonewithTA MMTof3+/5atIAtoFAof2/4LankleMAT

associatedwithimprovedFA4/5TAMMT.Subject4 reportswalkingfurtherthanshehadbeenabletoin years,correctingherleftanklefootdropattimes andfeelingencouraged.

Subject5(Leftlowerextremityspasticityandright lowerextremityspasmimpactedgaitusing3WW indoorhouseholddistancesforambulation)demonstratedimprovementbilaterallyinMAT,MMT, T25WTandTUGtestsfromIAtoFA.Subject5 improvedfromIATUG19.5sandT25WT15.34s toFATUG17.37sandT25WT13.53s.Healso demonstratedimprovementinanklespasticityfrom 3/4MATPFtonewithTAMMTof2/5atIAtoFA of2/4LankleMATassociatedwith2+/5TAMMT strength.Subject5alsodemonstratedreductionin allcategoriesofmeasuredspasticityatIAdownto 0/4exceptresidualbutreducedspasticityofbilateral PF(right1+/4andleft2/4)MAT.Subject5reports feelinghisleftlegimprovedslightlyinabilityto loosenhisleftkneeduringthetoe-offportionof walkingwhichallowedhimtousehishipbetterand walkfurther.Subject5statesplantoinvestina versionofelectricalstimulationduetohisresponse.

Subject6(Bilateralcaneswithintermittentrightankle footorthoticusewithweaknessandspasticitylimitingambulation)demonstratedsignificantimprovementinPFMAT,TAMMT,T25WTandTUG testsfrominitialassessmentto finalassessment. Subject6improvedTUGfrom54.75satinitial assessmentto16.81sat finalassessmentand T25WTfrominitialassessmentof36.88sto12.15s at finalassessment.Healsodemonstratedimprovementinanklespasticityfrom3/4MATPFtone withTAMMTof1+/5atinitialassessmentto final assessmentof2/4LankleMATassociatedwith improved2/5TAMMTstrength.Subject6reports feelingverysupportedbythevisitsandlearning howtoworkwithhisbodyaswellasimproved walkingespeciallyfrom10pmto11pmwithsignificantlyreducedspasticity.

Subject7(Rightlowerextremityspasticityassociated footdropcorrectedwithRAFOand4WWforhouseholdambulation)demonstratedimprovementinMAT inPF,MMTandmusclelengthbutwasunabletotolerateambulationtestingat finalassessmentdueto intoleranceinprobableurinarytractinfection.At RAsubject7demonstratesimprovementinspasticity, strengthandmusclelengthhoweveradeclinewith worsenedambulationspeed.AtIATUG46.15s andT25WT27.94swithRATUG47.5sand T25WT47.05s.AtIA,rightlowerextremityPF

spasticity3/4withassociatedrightTAstrengthof2/ 5.AtRArightPFspasticitydownto1+/4withassociated3/5TAMMT.AtFArightPFspasticity remains1+/4associatedwithtrendingimprovement rightankledorsiflexionof3+/5MMT.Subject7 reportsdespitethe finalappointmentbeingdisappointingduetohernewurinarytractinfectionsymptoms,thatshefeelsstrongerinherrightlegandmore comfortablewithwalkinginherhomeoverall.

InTable3,MATimprovementisdefinedasatleast1 gradedifferencefromIAtoFAonascaleof1+/4,2/ 4,3/4,and4/4.MMTimprovementisdefinedasat least1/2gradeimprovement.FromIAtoFAona scaleof0/5,1+/5,2/5,2+/5,3/5,3+/5,4/5,4+/5, 5/5.PertheAmericanPhysicalTherapyAssociation MultipleSclerosisOutcomeMeasureTaskforce, intraraterreliabilityforlowerextremityassessment insubjectswithMSis71.1%butneitherofthese assessmentshaveMCIDorMDCdatatoreference.9

Table3containsassessmentsforcategoricalmeasures shownbythecountandpercentageofpatientswho improved,stayedthesame,andworsenedovertime (frompretopost).

Discussion

Thiscaseseriesofsevenheterogenoussubjectswith MSisalowsamplesizeforstrongstatisticalanalysis ofresultsandshouldbeconsideredapilotstudy.The designisamulti-modaltreatmentapproachwhich demonstratesahighleveloffeasibilityandtolerance butcannotconcludecauseandeffectrelationships, onlypossiblecorrelationstofurtherinvestigate. Possibleotherexplanationsforresultsmeasured includeantispasmodic(baclofen)medicationin5 outof7subjects,thehighfrequencyofPTvisits, rapportwiththeprimaryinvestigatorandmultimodal interventions.Therearenoknownadverseresponses ofDCandantispasmodics.Baclofencombinedwith therapyinterventionsincludingelectricalstimulation improvefunctionaloutcomesinneurological patients.10

Trendsacrosssubjectsinspasticityandagonist strengthshowapossiblecorrelationbetweenmechanoreceptorinputfromDCallowinggreaterstretch andimprovedagoniststrength.71.4%ofsubjects demonstratedreductioninbilateralPFspasticity. 71.4%ofsubjectsimprovedMMTofbilateralTA. Twosubjectsstoodforthe firsttimeinmorethan3 yearswitheliminationofclonustheynormallyexperience.Subject6demonstratedsignificantimprovementinrightlowerextremityneuropathyand spasticityallowingsigni ficantimprovementingait

mechanicsandvelocityover25to50feet.Subject4 and5alsodemonstratedimprovementingait mechanicsandgaitvelocitywithreductioninspasticityandimprovementinagoniststrength.Subject3 experiencedFAworseningsymptomswhichcaused adeclineingaitmechanicsandvelocity.Impacton gaitisinconclusiveduetosamplesizeandtimeline.

Per findingsbyEtoometal.(SystematicReviewand MetaAnalysis)11 spasticitymaybebeneficialfunctionallybycounteractingmuscleweakness.11 The goalinspasticityreductioninterventionsneedstobe focusedonpreservingsafelevelsoffunction,while simultaneouslyimprovinganindividuals’ accessto normalizedanti-gravitysagittalandfrontalplane functionalmuscles.Thearticleindicatesthatthe bestavailablequalityofevidenceisforrobotassisted gaittrainingandoutpatientexerciseprogramson hypertonicity.This findingisinlinewithprevious research.12 Robotassistedgaittrainingcombines functionaltrainingwithelectricalstimulationdemonstratingreductioninspasticity.Thiselucidatesthe benefitofcombiningfunctionaltrainingwithelectricalstimulationtraining.1,6,11,13 Thereviewedarticlesdemonstratebenefitsinmuscletone,EMG muscleactivity,biomechanicalpropertiesofmovementandself-reportedspasmbutnoeffecton clonusordeeptendonreflex. 11 Per findingsinthis pilot,subjects1and2demonstratedeliminationof clonusduringandtemporarilyaftertreatment.This findingmaydistinguishthisversionofelectrical stimulation(DC)fromtheTENS(AC)assessedin thesystematicreview.Clonusisaphasicjerk responseasaresultofastretchattheextrafusal muscle fibersdetectedbythemusclespindleand transmittedtotheCNSbyIaafferentsthatconnect topropriospinal fibers.2 Inorderforclonusto reduce,themechanoreceptorsand/orCNSIa pathwayneedstohaveinputthatallowsnormalized stretch.

Summary

Basedonliterature findings,theuseofelectricalstimulationintheformofFESbikeandNMESisfeasiblefor personswithprogressiveMS.Thesetreatmentsmay reducefalls,improvemobility,reducephysiological deconditioningandreducespasticity.Inalloftheseoutcomes,furtherstudiesareindicatedtomakemoredefinitiveconclusions.3,14–17 Inthispilotwecanconclude thatinindividualswithprogressiveMS,DCisfeasible andwelltoleratedwhencloselymonitoredandmodified.ThisformofDCdemonstratesthepotentialto reducehypertonicityinformsofspasticityandclonus. Insomecases,thistreatmentalsodemonstrates

improvementinagonistmusclestrengthwithtrends towardimprovementinfunctionaloutcomemeasures.

TraditionalTENSapplicationmayreducespasticity throughthe1binhibitorypathwayshort-term1,18,19 and theformofDC5 usedinthiscaseseriesshowspotential toreducespasticityduringtreatmentandcarryoverafter treatments.Inthispilot,thisisdemonstratedintrendsof improvingagoniststrengthespeciallydorsiflexionwhile simultaneouslyreducingspasticity,especiallyatPF.Due tosubjects1and2beingnon-ambulatoryandsubject7 nottoleratingFAambulatorygaitspeedtesting,inconclusiveevidenceforgaitspeedimprovementisavailable. However,3/5ofambulatorysubjectsshowedtrends towardsimprovinggaitbiomechanicsandspeed.Most subjectsshowtrendstowardsimprovementinspasticity, strengthandROM.Subjects1and2withEDSS7.5–8.0 wereunabletoambulateorchangetheirdependenceona chairformobility,however,abletoinitiatefunctional standingduringtheirstudyparticipation.Thecase series findingssuggestfurtherresearchiswarranted.

Limitations

Theprimarylimitationsofthiscaseseriespilotstudy includeasmallsamplesizeandtreatmentscope limitedto18visitsover6weeks,whichlimitsgeneralizabilityofthe findingsandpotentialtherapeutic effectofthestudydesignonsubjectswithprogressive MS.Thestudyincludedaheterogenousmixofnonambulatoryandambulatoryindividualswithmultiple comorbiditieslimitingtoleranceoftreatmentand impactingassessments.Inherenttothestudyisa multi-modaltreatmentapproachwhichdemonstrates ahighleveloffeasibilityandtolerancebutcannot concludedirectcauseandeffectrelationships,only possiblecorrelationstofurtherinvestigate.Inherit biasmayoccurwiththestudydesignoftheprimary investigatorasprimaryauthorwithinacaseseries.

Futureresearchwouldbenefitfromisolatingtheeffect ofDConspasticityusingasingleordoubleblindrandomizedcontroltrialwithDCasthemaintherapyand alargersamplesizelimitingallsubjectstoeither ambulatoryornon-ambulatoryandperformedovera longerdurationtoincreasegeneralizabilityandvalidityof findings.Thisunfundedstudywaslimitedin scopetooneprimaryinvestigatorandone Neurologist.AllexpensesinInstitutionalReview Boardapprovalfeesandpublicationfeeshavebeen coveredbyNeurologicalFitnessEquipmentand EducationLLC,AustinTX.

Futurestudydesignsofelectricalstimulationorthis specificversionofDCshouldconsidercontinued

emphasisonfunctionalapplicationperformedsimultaneouslywithelectricalcurrentassessedwithobjectivemeasurementsinspinalreflex,spasticityand biomechanicalpropertiesofthegaitcycleinorder tobestunderstandquantityanddurationofreduced spasticitywithcorrelationintofunctionalchanges.1,6

Theauthorsdeclarenopotentialconflictsofinterest withrespecttotheresearch,authorship,and/orpublicationofthisarticle.TheuseoftheNeuBiewas approvedbyCenturaHealthbiomedicaldepartment. ThisDCunitisanFDAapprovedunit(AP439; HILLIFCInterferentialUnit;Neubie;Neuro-M TrainerModelA).CommonSpiritHealthResearch InstituteInstitutionalReviewBoardprovided approvalofthisstudydesignandconsentform (FWANumber:FWA00019514OHRPIRB Number:IRB00009715).

Declarationofconflictinginterests

Theauthorsdeclarednopotentialconflictsofinterestwith respecttotheresearch,authorship,and/orpublicationof thisarticle.

Funding

Theauthorsreceivedno financialsupportfortheresearch, authorship,and/orpublicationofthisarticle.

Disclosurestatement

NeuFithascoveredtheexpensesrequiredforinstitutional reviewboardapprovalprocessaswellasthefeesassociated withathird-partystatistician.Theinvestigatorshavenot receivedanyreimbursementforthisstudy.Theauthors declarenopotentialconflictsofinterestwithrespecttothe research,authorshiporpublicationofthisarticle.

NeuBiebyNeurologicalFitnessEquipmentandEducation LLC,AustinTX.

Manufacturer:JohariElectroTechCo.(Jodhpur,India).

ORCIDiD

CourtneyLEllerbusch https://orcid.org/0009-00071702-3574

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3.MillerL,McFadyenA,etal.Functionalelectrical stimulationforfootdropinmultiplesclerosis:asystematicreviewandmeta-analysisoftheeffectongait speed. AmCongrRehabilMed 2017;98:1435–1452.

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5.SalpeterG. Neubie:ANewParadigminElectrical Stimulation. https://neu.fit/reports/neubie-a-newparadigm-in-electrical-stimulation/.

6.WahlsTLandAdamsonE. TheWahlsProtocol:HowI BeatProgressiveMSUsingPaleoPrinciplesand FunctionalMedicine.NewYork:Avery,amemberof PenguinGroup(USA),2014.

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8.BuckmireAJ,ArakeriTJ,ReinhardJP,etal.Mitigation ofexcessivefatigueassociatedwithfunctionalelectricalstimulation. JNeuralEng 2018;15:066004.

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Appendix1(NeuBiedirectcurrentmapping)

AuniqueapplicationofNeuBiedirectcurrentis mapping.Thepractitionerplacesaredelectrode onthedistallumbosacralregionofthesubjectand usestheblackelectrodeleadattachedtoacarbon fi berelectrodewithwetspongeandscansacross thesurfaceoftheskinatcontinuous500Hz current.Thegoalistolocateareasofinterestindicatingorthopedichotspots,locationsthatallow increasedrangeofmotionfrombaseline,locations thatdemonstrateincreasedspasticityorspasm responseandlocationsthathavelowersensation thantheestablishedbaseline.Oncetheselocations areidenti fi ed,thepractitionercanplaceelectrodes tospeci fi callytargetorthopedicconcerns,range ofmotionimprovements,hypertonicregionsand neuropathy.

Appendix2(NeuBiedirectcurrentelectrical stimulationparametersusedinstudy interventions)

NeuBiedirectcurrentelectricalstimulationutilized forneuromuscularre-education,spasticityreduction andstrengtheningoflowerextremityandtrunk regionbasedonmappingandmotorpoints.Direct Currenttreatmentisalwayscombinedwith PT-basedinterventionsandfunctionaltraining lasting15–45mindependingontolerancewiththe followingparameters:

• Frequencyisadjustedbasedonpatientresponse from35–500Hzwithgoalofincreasingtime with500Hz.Inmid-rangefrequencyof35–75Hzthesubjectwasgivena1:4–1:2ratioof currentontocurrentofftimetoallowforrepolarization.Inhighfrequenciesof100–500Hzthe currentwasusedcontinuously.

• Intensityistobeadjustedtoremain70%–80% clientreportedperceivedintensityand/ortobe underthresholdofanyspasticityorpain.With electrodesinplaceemphasizingreductionin

spasticityandimprovementinrangeofmotion outofhypertonicpatterns,subjectsweregiven fl exibilitytreatmentforgastrocnemius,soleus, hamstrings,adductors ,quadratuslumborum, quadricepsandhip fl exors.Following fl exibility trainingsubjectsgivenAROM,AAROMand manualcuestoguidenon-compensatory agonistmovementtogreatestdegreepossible inmovementssuchasdorsi fl exionandeversion, kneeextension,knee fl exion,hip fl exion,hip bridging,hipexternalrotation.Next,subjects trainedathighestfunctionalmobilitychallenge theywereabletoperformwithcontrolledcompensationpossibleinbedmobility,sitting posture,sittostand,ambulationandstanding balance.

Appendix3(NeuBiedirectcurrentfootbath neuropathyprotocolusedinstudyinterventions) FootBathformultiplesclerosisperformedfor8–15minfortheaffectedside(ifbothaffectedthen treatedbothsimultaneouslywithdifferentchannels).Use500Hzcontinuouscurrent.**Assessed withtheclientoncomfortablerangeoftemperature forwaterandadjustedtemperatureaccordinglyfor comfort**

• Immersedtheentirefootandankleandhighankle inthewater.

• Providedthefollowingelectrodeplacementstarget thelocationsnerve fibersareclosesttothesurface. Thelumbarnerverootsto filloutasmuchofthe sciaticandfemoralnervepathwaysandmany bifurcationsaspossible

• channel1:Lumbarnerverootstotheaffected sideverticalelectrodeplacement(red)

• channel2:Midadductorverticallyplacedelectrode(red)

• channel3:Poplitealfossahorizontallyplaced electrode(red)

• channel4:Distalquadhorizontallyplacedelectrode(red)(allblackelectrodeswere floating carbon fibersinthewater,keptoutoftheway ofthetreatedfoot/feet.)

Duringfootbathprovidedmanualactivationsfor lowerlegmuscles;talocruralglides(toencourage thecalcaneousremainincontactwith floorsurface) grade2–4dependingontolerance,metatarsalglides, proximalphalanxglidesandbilateraltendon massagejustlateraltoachilles.Withlocationsthat allowedforgreaterdesiredankleorfootROMcontinuedtoworkandhaveclientmovethroughimproved rangeastolerated.Withareasthatcausereflexive response,decreasedtheintensityofmanualwork belowathresholdthatelicitedthereflexand workedwithclientondiaphragmaticbreathingwith

thework.Aftermanualworkassessforqualityof movementespeciallyintoankledorsiflexionand eversion.Whennoticingcompensationintoinversion orweaknesswaspresent,thenprovidedeither AAROM,isometricholdoreccentriccontrolinto

dorsiflexionandeversionandhadtheclientmove withcorrectionorsustainaposition.Throughout thisprocessensuredthecurrentwasatjustbelow thelevelthatwouldcausespasticityand/or7/10 intensity.

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