Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post-

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Biological Control 62 (2012) 53–63

Contents lists available at SciVerse ScienceDirect

Biological Control journal homepage: www.elsevier.com/locate/ybcon

Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post-harvest strawberry fruits R. Huang a,b, H.J. Che a, J. Zhang a, L. Yang a, D.H. Jiang a, G.Q. Li a,⇑ a b

State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China Plant Protection Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, Jiangxi Province, China

h i g h l i g h t s

g r a p h i c a l a b s t r a c t

" We evaluate Sporidiobolus pararoseus as biocontrol agent of Botrytis cinerea. " The yeast could not inhibit growth of B. cinerea in dual cultures on agar medium. " The yeast cells effectively suppressed B. cinerea in infection of strawberry fruits. " The yeast produces volatiles highly effective in suppression of B. cinerea. " We conclude that S. pararoseus is a promising biocontrol agent against B. cinerea.

a r t i c l e

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Article history: Received 6 December 2011 Accepted 23 February 2012 Available online 3 March 2012 Keywords: Sporidiobolus pararoseus (strain YCXT3) Botrytis cinerea Strawberry Volatile organic compounds Biological control

a b s t r a c t This study evaluated Sporidiobolus pararoseus (Sp) strain YCXT3 as biocontrol agent of Botrytis cinerea (Bc), the causal agent of strawberry gray mold disease. Efficacy of live yeast cells and volatile organic compounds (VOCs) of Sp in suppression of Bc on strawberry fruits was determined. Results showed that in dual cultures of Sp and Bc on potato dextrose agar at 20 °C, Sp did not inhibit mycelial growth of Bc. However, inoculation of the yeast cell suspensions of Sp (1 105 or 1 106 yeast cells ml 1) on strawberry fruits resulted in reducing the disease incidence from 96–100% in the control treatment to 39–50% in the Sp treatment and the disease severity index from 5.1–7.0 in the control treatment to 1.1–1.9 in the Sp treatment. We found that the VOCs from the Sp cultures on yeast extract peptone dextrose agar were highly effective in inhibiting both the conidial germination and the mycelial growth of Bc. A total of 39 VOCs, including 2-ethyl-1-hexanol, were identified in cultures of Sp using GC–MS. Authentic 2-ethyl-1hexanol was found to have strong anti-fungal activity against Bc with the IC50 values of 1.5 and 5.4 ll l 1 for conidial germination and mycelial growth, respectively. The VOCs from the Sp cultures were effective in suppression of gray mold disease under the air-tight conditions. This study suggests that the strain YCXT3 of Sp is a promising agent for control of Bc and production of VOCs is a valid biocontrol mechanism for this yeast strain. Ó 2012 Elsevier Inc. All rights reserved.

1. Introduction Botrytis cinerea Pers.: Fr. (teleomorph: Botryotinia fuckeliana (de Bary) Whetzel) is a is a plant pathogenic fungus distributed

⇑ Corresponding author. E-mail address: guoqingli@mail.hzau.edu.cn (G.Q. Li). 1049-9644/$ - see front matter Ó 2012 Elsevier Inc. All rights reserved. doi:10.1016/j.biocontrol.2012.02.010

world-wide and causes gray mold disease on both pre-harvest and post-harvest plant tissues (Williamson et al., 2007). It infects more than 200 species of plants comprising many economically important crops such as cucumber (Cucumis sativus L.), table grape (Vitis vinifera L.), tomato (Lycopersicon esculentum Mill.) and strawberry (Fragaria ananassa Duch.) (Williamson et al., 2007). Yield loss caused by B. cinerea on these crops can be severe under cool and humid conditions (Yu et al., 2006; Droby and Lichter, 2007).


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Evaluation of Sporidiobolus pararoseus strain YCXT3 as biocontrol agent of Botrytis cinerea on post- by Eduardo Santiago - Issuu