2021/9/17 下午2:25
Applications of Calcium Imaging Technology in Nervous System Research - Creative Biolabs
Neurons will burst out a short calcium concentration peak when they are firing, which means that the calcium concentration of neurons can represent synaptic transmission and neuronal activity [1].
Fig.1 Neuron signal transmission.
Therefore, the principle of neuronal calcium imaging technology is to rely on the strict
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correspondence between calcium concentration and neuronal activity. By using special fluorescent dyes or protein fluorescent probes (calcium indicator), the concentration of calcium in neurons is displayed by fluorescence intensity and captured by a microscope to achieve the purpose of monitoring neuronal activity. The commonly used gene-encoded calcium indicator is to combine the fluorescent protein derived from green fluorescent protein (GFP) and its variants (such as cyclic array GFP, YFP, CFP) with calmodulin (CaM) and myosin light chain Kinase M13 domain fusion. When the calcium concentration increases, it will cause M13 to bind to CaM, thereby changing the conformation of cpEGFP, changing it from a non-fluorescent state to green fluorescence.
https://neuros.creative-biolabs.com/applications-of-calcium-imaging-technology-in-nervous-system-research.htm
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