Creative Biolabs| Lipid-based Drug Delivery
GET INSIDE THE BRAIN! Liposomes for brain drug delivery Expert in Lipid Based Drug Delivery Services
Creative Biolabs| Lipid-based Drug Delivery
Liposome Development Services
Contents • Background • Pathways for crossing the blood-brain barrier (BBB) • Liposome-based strategies across the BBB • Creative Biolabs’ liposome development services
Creative Biolabs has more than ten years of experience in liposomal formulation development. We integrate the knowledge of well-known liposome academics into our liposome technology. Our team of liposome experts and support staff has successfully served thousands of customers all over the world. At Creative Biolabs, we provide a full set of liposomes custom services and offer various liposome analysis services to characterize liposome-based products and measure the embedding efficiency. We believe our liposome products and services will meet the highest standards.
Creative Biolabs| Lipid-based Drug Delivery
Background The blood–brain barrier (BBB) is a highly complex multicellular structure that protects the brain from harmful substances and invading organisms from the bloodstream, supplying brain tissue with nutrients and controlling its homeostasis. The BBB is composed of polarized endothelial cells connected by tight junctions of the cerebral capillary endothelium and a variety of transporters, which are responsible for its extremely low permeability, limiting the delivery of drugs to the central nervous system (CNS). BBB functionality is dynamically regulated by an ensemble of different cell types, such as astrocytes, pericytes, and neurons. Endothelial cells are surrounded by a basal lamina, which is generally rich in laminin, fibronectin, type IV collagen, and heparin sulfate, representing an interesting target for drug transport and providing a negatively charged interface. The development of new strategies to treat brain diseases is challenging, considering the low drug accessibility to the brain due to the presence of the BBB. In several high incidence brain pathologies, such as stroke, brain infections, Alzheimer’s disease (AD), and Parkinson’s disease (PD), the BBB is altered. Specialized BBB components are more permeable if failed to maintain, allowing the molecules that cause neuroinflammation and neurodegeneration to enter the brain. However, even when damaged and more permeable, the BBB can still be difficult for drug delivery into the brain. Fig 1. Cross-section of a cerebral capillary
Creative Biolabs| Lipid-based Drug Delivery
Fig 2. Different drug delivery mechanisms across the BBB
CMT
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Carrier Mediated Transport carbohydrates, fatty acids, amino acids, nucleotides, hormones, vitamins, organic cations and anions
AET
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Active Efflux Transport
RMT
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Receptor Mediated Transport peptides and proteins
Creative Biolabs| Lipid-based Drug Delivery
Liposome-based strategies across the BBB Liposomes are a type of nano-sized phospholipid bubbles that have attracted much attention as potential drug carriers. Liposomes are highly biocompatible and easy to control in vivo. Due to their unique physicochemical characteristics, liposomes can incorporate hydrophilic, lipophilic, and hydrophobic therapeutic agents, including a broad variety of drugs, DNA, and diagnostic agents. Hydrophilic compounds may either be entrapped into the aqueous core of the liposomes or located at the interface between the lipid bilayer and the external water phase. Lipophilic or hydrophobic drugs are generally entrapped almost completely in the hydrophobic core of the lipid bilayers of the liposomes.
Advantages of Liposome: • • Fig.3 Structure of liposomes. (Débora B Vieira, 2016)
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Nano- or microsize vesicles Able to incorporate hydrophilic, lipophilic, and hydrophobic therapeutic agents. Good biocompatibility and biodegradability, low toxicity, drug-targeted delivery, and controlled drug release.
Creative Biolabs| Lipid-based Drug Delivery
Cationic Liposome
Ligand-targeted liposome
Liposomes are used to treat:
Administration Routes:
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Alzheimer’s Disease Parkinson’s Disease Stroke Glioma
Intravenous injection Intraperitoneal injection Oral administration Intranasal administration
Stimuli-Responsive Liposome
Creative Biolabs| Lipid-based Drug Delivery
Creative Biolabs’ liposome development and analysis services
Creative Biolabs| Lipid-based Drug Delivery
Our custom liposome services can regulate the solubility of encapsulated small organic molecules, protect the embedded agents from degradation, and achieve site-specific delivery. Our services include liposome formulation design, liposome encapsulation, and liposome-drug complex analysis and characterization. We provide various novel liposome formulations including immunoliposomes, proteoliposomes, magnetic liposomes, fluorescent liposomes, and pH-sensitive liposomes.
Features of Our Custom Liposome Services: • • • • • •
Non-toxicity, biocompatible, and completely biodegradable Increased drug efficacy Site avoidance effect Increased stability Reduced drug toxicity Flexibility for active targeting
As a liposome contract manufacturer, Creative Biolabs is making high-end liposomal products for our customers. We can design and produce a liposomal product that is fully tailored to your requirements. Our groundbreaking research, objectified working process, and state-of-the-art equipment mean that we can develop distinctive liposomal formulations and products. Our customized liposome services offer genuine phospholipid bi-layered vesicles that contain the active substance. We can produce liposomal formulations for any active substance. Besides, our services make every liposome unique and are subject to stringent quality control. We guarantee that our liposomes retain their quality and efficacy during storage. For more details about our services, please contact us.
Creative Biolabs| Lipid-based Drug Delivery
CONTACT SUITE 203, 17 Ramsey Road, Shirley, NY 11967, USA Tel: 1-631-871-5806 (USA)
44-207-097-1828 (Europe) Fax: 1-631-207-8356 Email: info@creative-biolabs.com