2021/9/17 下午2:25
Calcium Indicators
Applications of Calcium Imaging Technology in Nervous System Research - Creative Biolabs
Features
Applications The sensitivity is more than five times that of
jGCaMP7s
Sensitive and slow
GCaMP6s, which is suitable for the detection of single action potentials. The sensitivity is more than five times that of
jGCaMP7f
Fast kinetics
GCaMP6f, which is suitable for the detection of single action potentials. The sensitivity is 3 times that of GCaMP6s, and
jGCaMP7b
Brighter baseline
the fluorescence brightness is increased by
fluorescence
50%. It is suitable for detecting neuronal processes or nerve fibers.
jGCaMP7c
High contrast with low
Clear signal, enhanced contrast, suitable for
baseline fluorescence
wide-zone imaging
0
3. XCaMP Series XCaMP is obtained through a series of mutation screening using CaMKK as the backbone. XCaMP includes four different colors: blue XCaMP-B, green XCaMP-G, orange XCaMP-O, and red XCaMP-R. Among them, the fluorescence intensity of XCaMP-G is stronger than that of GCaMP6, and the performance of calcium response induced by action potential stimulation is also significantly improved than that of GCaMP6. Combined with specific neuron-specific expression methods, the activities of three different neuron types in specific behaviors can be simultaneously monitored in the state of free activity; combined with two-photon microscope, microstructure functional imaging can be realized to achieve the Two-color imaging of the rear structure at the same time [7]. 4. CaMPARI A new type of calcium imaging technology that can take into account both the global and the micro, including CaMPARI and CaMPARI2 (second generation) Principle: CaMPARI protein emits green fluorescence under normal conditions, and if this protein is treated with high-concentration calcium and ultraviolet light at the same time, it will irreversibly and permanently transform into another confirmation that emits red fluorescence. The researchers introduced this new type of protein into the nervous system of experimental animals through genetic modification and then irradiated the animal’s brain with high-intensity
https://neuros.creative-biolabs.com/applications-of-calcium-imaging-technology-in-nervous-system-research.htm
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